Cucsat.G8004.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8004.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	3-isopropylmalate dehydratase large subunit, chloroplastic isoform B
Location	ctg1556: 2243632 .. 2246600 (-)
RNA-Seq Expression	Cucsat.G8004.T2
Synteny	Cucsat.G8004.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGATCTGTCTTCCTCTTCCTCCTCAAGAAAAACGACGAACCTGAAAAACCTCCTCAGCTTCAAGGTTCCCGGAGCTTCATTGCAGAATGGATTCGGCCTCCAAATTCCTTCGTAGTTTAGCTAATGCCACCAACAAAAACACCGTTATCAACGTCTGTTTAGTCGTTTCCTTCGCCGCACTGACCGCAAGGTCAATCAAGCAGGAGAGACAAATTGAAGCTCTGGAAACCGAGAAAAATTCACTTCTCAATTCCAATAAAGCGTTGAAGAAAACCATGTGGGATTGGAAGCAACAGCTATTCGCCGAAGCCTCAACCGAATCTGCTTTAGTCCCTCTTGCCAGGATCAAAGCTATCTATGGCGAAGCTCCCATTTCCCCTTCCGGTAATATACGCCATGCTTCTCTCTGTTCTGTTCTTTATGTTTTTTTACTTTCTGTTGTTGGAAAAGTTAGTGTTTAGTTGAATGTGTCGAAATGTTAGTGATTCGTGTTAGAAATTGAGTGTCTGAAAATGATGGGTCGTTCCAAGCATTTCAAATTCCTTATTCGTACTTTACAAATTGTTGGGTTTCGTTGCTACTTCAACTATATCATCATTGACCTGATTTTTCTTATCATCCATTTCTGAACTTCAATGTTGTTATGATTAAGTACCATGTTCTTGGTTTTCATGGATTCTAATCTCTCAGAACTATTAGCCAGAACCCAGGAAACAAATAAGGTATGAGATTATGAATCTAAAAAGAATAGTTTTCATCGCACCATTATATGAAAAAAAGTGTGTTTGAGAAGTGAATGTTCTATTATGGGATGCTGGAATTGAAGACTAAAGTTAAGATTGATAAGGTCAACAGAACCTCCACCTATCTTCTACCTTGGCTATAAACTCGAACTACCCAAATGGTCTCGTACTAATTAAGATAATTGTTCTCACTCATGTACTAATGATTGTACTCATGTTTGCCCTAAAGATAATCGATAAAGAAGGAACCATACCCAGCCAAGCCTAAAGCCACTGAGCAATTCTCTACCTTCATCCTTTCCTACATTTCAATTTACGAATCACCTTGACAACTTTCACCCTGATAGACCATGCTGATCATTCCTGTTCTCTGCCTATTAAGTTAACGTTCCTGCCCAGCTGATGCTAAGGTTTTCATTATGGGGATAGTATATGCTGTATTAAACTTTACCGGGAAAGCACTGCTATAGTACTACGATTTTATATGAGAAAGAGACGCTTGAAAAGCTTATATGCGAAAGGAAGCAATTTCTCTTATTGGAAGCTTAAGAAAGGCACTCCAATTGAAGTTGATTGTGAAGGCAGAATAGTCATAGATGATAGGAGGAGTTGGAAGGGGAAGCTAAAAGACCATTGAACTTTTCCAATTCTTGAACGTCTTCCCCATATCTCTCCCTATTATTAGAAATCTGTTAATTTCTTTCTTTCTAGCCACCAGCCACTATACCTAATCCTGGACTGCATTTTGCTATAGGATCTCTCTCCTTTCGTAGTTGGTTCAGCACAGAAGCTGCATCTAACTATCCTTGGTCCGTAAATAAAAAACCGAGAAAATGATGATGTAGAGGAAAACTAGTAAAAGGCTTTGTGTCTTCTTTGGATTCCTACATAGTATGCACCAACTTAACTTGGTTGCAAGATCAGCAATTGTCATCTTCAAATTGGATTTTTGTATCTCCATTGTGTTTAGGTCAAGACACACAAACCAACAATATCTCTCGATTTTTATAAATTGGTTACCTTTCCAATTTGATAGTGATAAGTTTCTAGGTAGTGAGGAAACCAGTCTGCTATCTAGCTGTCATTGATCAACAGAAATGCGTTGCTTTCGTCAACCTTATGATAATATTACCCTGTTGACGATGACTCTAGTATGTATTTCGTAACTTTTGGGGAGTTATGAAGATCTTTGCTCAAGAATTGGTACTAAAATGTCTGCTTTTAGTACAAACGGATTGTAATTTCAGATAGATTGGAAGATAAACAGCACGAGTAGATTTTTTGAAAAAAAAGATTCAAATGCATGTTTGATGCAAAACTTTTACCCTTTTTCCTGGCAGAGTTCTTGCATAACAATGTTGGGAATCTATTAAACGATTGCCTATAAATTAATAATCGTAATGTAATGTGAGAGTTTTAAGTCTCGATTCAGATTATGTCAAATCCTATCCATATTATTTCATTCTCCCATGCATATTATTTAGTTATTGAACACATGAATCAAATACTCTTGTGGATAGTATTTTTTCAAGTTTGATTACAAAAAGAAGTGTTTGTTTAGAAAGAGTTTTAAGTGTCTAAAAAGTCATTTTGAATATGTTCAAGTCTATTATTGTACAGGCTAGCTTTTACAGATTTATCCATTGATCCCTTTTTTATGGACCATACAGGAGCAGTAAATGCCACAACCGAAGATGCAACTTCACGAAGCTCCAAACTTATGGTTTAATGTTAGCTTCACTCAAGTTTTCCAGGTTTTGTTTCCTTTGTTCACAAATTGGCTGAAGAATCGAACTCCATTAAGATTTGCATATTTCTATGCTCAATGCTAAAAAAAGTAGTGCAGAATATAAGGAATTAGATTTTTCCTTCTTCAGGCACGTATTATCAACTTCAAAGTTAGATATCATATTCCCTACCCTCACATTTTCAGAAGGAAAAAAGAAAAAAAAATCTTTTAGTTTGCCATTAAATGCTCTAGAATAAAACAGCTGATGTAGTCAAAAGCTCCATGATGACAAAGCATCATTATACAACCTTTTGCAACTACTTTAAAATAGGTTAAAGTGTGGTTTTAGTCTCGCACCTTGAAGATTGTTTAGGTTTAGTTTCTTTTTAATCTTCGCGCTTTTTATAAATTTTAAAGTTAATTTGTTATTGTTGATAAATACTTGTTATTATCTATTAGCATTTTTACTATAGTTTTGAAAACGCATAAGCATTT

Coding sequence (CDS)

ATGGATTCGGCCTCCAAATTCCTTCGTAGTTTAGCTAATGCCACCAACAAAAACACCGTTATCAACGTCTGTTTAGTCGTTTCCTTCGCCGCACTGACCGCAAGGTCAATCAAGCAGGAGAGACAAATTGAAGCTCTGGAAACCGAGAAAAATTCACTTCTCAATTCCAATAAAGCGTTGAAGAAAACCATGTGGGATTGGAAGCAACAGCTATTCGCCGAAGCCTCAACCGAATCTGCTTTAGTCCCTCTTGCCAGGATCAAAGCTATCTATGGCGAAGCTCCCATTTCCCCTTCCGTAAATGCCACAACCGAAGATGCAACTTCACGAAGCTCCAAACTTATGGTTTAA

Protein sequence

MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKALKKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSVNATTEDATSRSSKLMV

Homology

[Close]

BLAST of Cucsat.G8004.T2 vs. NCBI nr
Match: XP_004151748.1 (uncharacterized protein LOC101219754 [Cucumis sativus] >KGN54811.2 hypothetical protein Csa_012251 [Cucumis sativus])

HSP 1 Score: 213 bits (543), Expect = 2.27e-69
Identity = 116/118 (98.31%), Postives = 116/118 (98.31%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL
Sbjct: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS  VNATTEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. NCBI nr
Match: XP_008444149.1 (PREDICTED: uncharacterized protein LOC103487583 [Cucumis melo])

HSP 1 Score: 192 bits (487), Expect = 7.87e-61
Identity = 104/118 (88.14%), Postives = 109/118 (92.37%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR L+N  NKNT+INVCLV SFAAL+ARSIKQERQIEALE EKNSLL+SNKAL
Sbjct: 1   MDLASKFLRILSNDNNKNTLINVCLVFSFAALSARSIKQERQIEALEAEKNSLLDSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPS  VNA TEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTQSALVPLARIKAIYGEAPISPSGAVNAATEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. NCBI nr
Match: XP_038897052.1 (uncharacterized protein LOC120085226 [Benincasa hispida])

HSP 1 Score: 178 bits (451), Expect = 2.42e-55
Identity = 95/118 (80.51%), Postives = 105/118 (88.98%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR++ NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK SLLNSNKAL
Sbjct: 1   MDLASKFLRTVTNATNNNTLINVCLVLSFGALSARSIKQQREIEALEAEKVSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSV--NATTEDATSRSSKLMV 116
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPS   +  TEDA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTDSALVPLARIKAIYGEAPISPSGVGHVATEDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. NCBI nr
Match: XP_022976887.1 (uncharacterized protein LOC111477117 [Cucurbita maxima])

HSP 1 Score: 175 bits (443), Expect = 4.01e-54
Identity = 91/118 (77.12%), Postives = 104/118 (88.14%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR+L NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDLASKFLRTLTNATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQL++EAST+SAL+PLARIKAIY EAP+SPS    A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSEASTDSALIPLARIKAIYSEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. NCBI nr
Match: XP_022937146.1 (uncharacterized protein LOC111443531 [Cucurbita moschata] >XP_023536544.1 uncharacterized protein LOC111797682 [Cucurbita pepo subsp. pepo] >KAG7024766.1 hypothetical protein SDJN02_13584 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 171 bits (433), Expect = 1.34e-52
Identity = 88/118 (74.58%), Postives = 105/118 (88.98%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR++ +ATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNK+L
Sbjct: 1   MDLATKFLRTVTSATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKSL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQL+++AST+SALVPLARIKAIYGEAP+SPS    A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSDASTDSALVPLARIKAIYGEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL
Match: A0A1S3B9R7 (uncharacterized protein LOC103487583 OS=Cucumis melo OX=3656 GN=LOC103487583 PE=4 SV=1)

HSP 1 Score: 192 bits (487), Expect = 3.81e-61
Identity = 104/118 (88.14%), Postives = 109/118 (92.37%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR L+N  NKNT+INVCLV SFAAL+ARSIKQERQIEALE EKNSLL+SNKAL
Sbjct: 1   MDLASKFLRILSNDNNKNTLINVCLVFSFAALSARSIKQERQIEALEAEKNSLLDSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPS  VNA TEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTQSALVPLARIKAIYGEAPISPSGAVNAATEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL
Match: A0A0A0L380 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G364020 PE=4 SV=1)

HSP 1 Score: 190 bits (483), Expect = 1.88e-60
Identity = 100/101 (99.01%), Postives = 100/101 (99.01%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL
Sbjct: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSVN 101
           KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS N
Sbjct: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGN 101

[Close]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL
Match: A0A6J1IGY3 (uncharacterized protein LOC111477117 OS=Cucurbita maxima OX=3661 GN=LOC111477117 PE=4 SV=1)

HSP 1 Score: 175 bits (443), Expect = 1.94e-54
Identity = 91/118 (77.12%), Postives = 104/118 (88.14%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR+L NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDLASKFLRTLTNATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQL++EAST+SAL+PLARIKAIY EAP+SPS    A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSEASTDSALIPLARIKAIYSEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL
Match: A0A6J1F9J6 (uncharacterized protein LOC111443531 OS=Cucurbita moschata OX=3662 GN=LOC111443531 PE=4 SV=1)

HSP 1 Score: 171 bits (433), Expect = 6.49e-53
Identity = 88/118 (74.58%), Postives = 105/118 (88.98%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR++ +ATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNK+L
Sbjct: 1   MDLATKFLRTVTSATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKSL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPS--VNATTEDATSRSSKLMV 116
           KKTMWDWKQQL+++AST+SALVPLARIKAIYGEAP+SPS    A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSDASTDSALVPLARIKAIYGEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL
Match: A0A6J1CGB8 (uncharacterized protein LOC111011272 OS=Momordica charantia OX=3673 GN=LOC111011272 PE=4 SV=1)

HSP 1 Score: 166 bits (420), Expect = 6.40e-51
Identity = 88/119 (73.95%), Postives = 101/119 (84.87%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR+L NA+N  T+INVCLVVSF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDMATKFLRTLTNASNNKTLINVCLVVSFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSV---NATTEDATSRSSKLMV 116
           KK+MWDWKQQLFAEAS+ESALVPLARIKAIYGE PIS      +A TED  S+ SK +V
Sbjct: 61  KKSMWDWKQQLFAEASSESALVPLARIKAIYGEVPISSPTGAGHAATEDENSQGSKFVV 119

The following BLAST results are available for this feature:

BLAST of Cucsat.G8004.T2 vs.


Match Name	E-value	Identity	Description

BLAST of Cucsat.G8004.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151748.1	2.27e-69	98.31	uncharacterized protein LOC101219754 [Cucumis sativus] >KGN54811.2 hypothetical ...	[more]
XP_008444149.1	7.87e-61	88.14	PREDICTED: uncharacterized protein LOC103487583 [Cucumis melo]	[more]
XP_038897052.1	2.42e-55	80.51	uncharacterized protein LOC120085226 [Benincasa hispida]	[more]
XP_022976887.1	4.01e-54	77.12	uncharacterized protein LOC111477117 [Cucurbita maxima]	[more]
XP_022937146.1	1.34e-52	74.58	uncharacterized protein LOC111443531 [Cucurbita moschata] >XP_023536544.1 unchar...	[more]

BLAST of Cucsat.G8004.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B9R7	3.81e-61	88.14	uncharacterized protein LOC103487583 OS=Cucumis melo OX=3656 GN=LOC103487583 PE=...	[more]
A0A0A0L380	1.88e-60	99.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G364020 PE=4 SV=1	[more]
A0A6J1IGY3	1.94e-54	77.12	uncharacterized protein LOC111477117 OS=Cucurbita maxima OX=3661 GN=LOC111477117...	[more]
A0A6J1F9J6	6.49e-53	74.58	uncharacterized protein LOC111443531 OS=Cucurbita moschata OX=3662 GN=LOC1114435...	[more]
A0A6J1CGB8	6.40e-51	73.95	uncharacterized protein LOC111011272 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 29..63
None	No IPR available	PANTHER	PTHR38355	OS06G0149500 PROTEIN	coord: 1..116

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8004	Cucsat.G8004	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T2.E1	Cucsat.G8004.T2.E1	exon
Cucsat.G8004.T2.E2	Cucsat.G8004.T2.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T2.C2	Cucsat.G8004.T2.C2	CDS
Cucsat.G8004.T2.C1	Cucsat.G8004.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T2	Cucsat.G8004.T2-protein	polypeptide