Cucsat.G7989.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G7989.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Sec14p-like phosphatidylinositol transfer family protein
Location	ctg1556: 1814821 .. 1818418 (-)
RNA-Seq Expression	Cucsat.G7989.T2
Synteny	Cucsat.G7989.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGTTTTTTTGCCTTCAAGAACTTCCATTTCAGCAAAATTCTGGATACCCAAGAATACGATGACAAGTTGAGCTGTTGAATTTACTGACTTTTGAAAGGAAAGGAATCCAAATCACTCGAAAAGGAAAATCCATAAAAAGAAAATAAGAAATAAATTAATGGCATTTTCCAATTTCCCCGCTTTGAACTTTCAAGTGTTGAATTACCCAAATCACCAAACAGTACCGACAAGAACTCGAACCCCCTACACGGTGTCATCAAAATCACTCTTTTTCAAAGCAATTGAATCTGGATTTCAAAGGGTCTCCCCCAACTCCAATCCTTCAACCCTCTCTTCGCTAATTCCTCTCAGCTGATAATTCATTTTATCCATTTTCTTCACACTTACTTACTCTACGCTCTTCACTGATGATACCCTTTTTCTCTCTTCAACTACTTTTGTATGGTTTGAACTTCCAAGAATTCGCCCATCCAACCAATACCCACTTCCATTGATTTGGTATATTTTTTTGTTTTAATTGTTAACCTTTGATTTATCATACTTTTGCCTAGCTTTATGAAGTTGTATGGGTTTCTATGTCTACCTTTCTGAAGTTTCCTTCGTCATTTTTCGATGCTAGATCAAATATCTGAAGGAAGATTGTTTTTTTTTTTTTCATGTATGGTTTAGCTTTACATCCCATGTACTTTGAATGGAGAATTAACTATGTCATTTTGAGGTTGATGGTGGAAAACAGACAACATAGGGCACCCATTTCTCCGTTTAACGACTGAAAGAAAACCTCTTCATGTTGTGGATTGTGAAGACATTATTAGTCAGTTTAATGGGAGTACTAGAGGTGGCACTTTGTCTATATCTAAGTTTAATTTTCTAAGAAAAGGTGGAATTGATGAATTTTCCAACTGTTTAGTGCAATATTGGATATCAGTAGTTGAAAGTATTTGAGTCCTTATGATTTATAATCTTGATCATGATAATAAGTTGCAAGGACTGTTCAAATTGTGATAGTTAACATTTTGTTACTTCATTGATAGTGAAGCTTAAAAAACTAGAAATCAACAGTGTTGTAGTTAACATTTTATTAATTCTTATTATGTGTATAGATCCTTTCATCAGATCTTTCAAGCTTTATAGTTTGTGGGTGAGATCTCTCTTCTTGTGCGTGCCTTTTAGATGCTTCTTTTGTTATCGTTAGTTCATGCATCATCTTTTGCCATATTGGTAAGTGTTCTTGAGATACAGCATATATGCTCTCTTCTCTATCTGAAATAATGAAATTCTCTTTGTTGGGTAGTTTGAATTATAAAAAAATGTGGTACAAACAGGTATTGAGTCTTGGAATTCTGGAGGCTGCTTAAATCTGAAATTTGTTTTTTGGCCAGGTGCACAATTCAAAAACCATGTCTTTAAATTTAGAGGTACCTCCTTCAAATGGTTGTGAGAAAGTTATATTATCTGAAGAACAGCATATGAAGGTATTTTTGACAGTTACAGCATTTTTTAGCCTCTTCATACCAGTGCTAACTGTCGATTGCTGCTAATCTTCTTGTGCGTGACTAAGAAGGTTGAAATGCTGCATTTTTCTAGTTGCTTAACTCTTACAGTTTTCCTTTATTTCATCCTACACAGATTTGTGAGGTGCGAAGGCTTTTAGGACCCTTGTCGGGTACGTCATCAATTTACTGTTCGGATCTATCCATTTTGAGATATCTAAGAGCAAGAAACTGGAATGTGAAGAAGGCAACTAAAATGCTGAAAGCAACTTTGAAGTGGAGGTCAGAATACAAACCCGAAGAGATTCAATGGGTACATTACTGCTTACTTTTGCCAAATCTTTCTGAAGTCATGCCCATAAGCTCAAAACGACTCGGTCTTATTCTTTCTTGTTAGCTACCTGGCTTTTTTTGTTTCAATACGAAGAGGAAGCTATTACTTAATTCAATTTCTGTTGTTTTCTGCTTTTTTATCCTTCTTTTGCCACATTTTTTTTTCAATTCAAAGGGTTCTATATGTTGTAATAGTTCTTGTTTGACAAGGTTTTAATTCTCTACAAGCTATGGTGTTATATGCATTCAATAGTTAGATCTTTATTGCAGGATGAGGTTGCTCATGAAGCTGAAACTGGGAAAGTTTATTGTGCGGACTGTAAGGACCGACATGGAAGAACAGTGATAGTCATGAGACCTTGTCGCCAGGTTTCATTTGCATTATGCAGTCCTAACAGAAATCTACTGGACATTTCTGAATTTTGGAAGTTCTTGTTGCTTGTAGCTATTTTAGAAATAAGTGCTGGGGATGGGGTAGTGAGATTGTCTGGCACATTGACTCTTATTAATGAAGATGTTTTTCATTAAGTGAATGAAATGTTTTGACTTATATATTTTAAATGGGATCTCTAATGAATAAGCAAACTTGGAATCCTTTATATTTAGCTTCCAAACAGCTAGCTTAACAAGTTCGTGTTTTCCGTCTCAACCACAGAACTCGAAGACTGTAAAAGGACAGATTAGATACTTGGTGTATTGCATGGAGAATGCTATTTTGAATCTTCCATCAAACCAGGAGAGTATGATCTGGCTGGTTGATTTCAAGGATTTTGACATGTCAAATATCTCATTAAAAGCAACAAAGGAGACTGCACATATTTTACAAGAACATTATCCCGAACGCCTCGGATTGGCAATTCTGTATAACGCGCCCAAGTTTTTTGAACCCTTCTGGATGGTATAATTTATTTGAAGAACTAACTTTATGGTTTCTTGTCATTCAAATATCTGAATTTACTGTACATCTCACTATGATTTGTGGCCCATAGGTGGGAGTAGTAGTAGTACACTGCCATTTCTGTTTTACTTGCTGCAATTATTTGTTGTTTACACTACTGGACAAACGTACTGTTCCATTTACAGGTTGCAAAGCCTTTCCTGGAGACAAAAACTGCCAACAAAGTGAAATTTGTTTACTCTGACGACGTAAACTCAAAGAGGATAATAGAGGATCTGTTCGATATGGACCAGCTGGAGTCTGCGTTTGGTGGAAACAATAATGTTGGTTTCAATATAACCAAATATGCTGAGATGATGAAAGAAGACGATAAACAGATGCGTTGTTCCTGGTCCGCGGTAAATCCTAATAGCCTAGCCTCGGAAAAAGCTCAGAGTAGCGGTCTAGTCGGTTCTCAAATCACTAACCGTTCTGAATCCTCAGAGAATGAGAGGACAGATGGTGATATTCATATAACTAGAAAAGCAGATGGCAACTGAGGCAGAATTCAAGTTAACTCATATAAGTTTGAGTCTGATTTTTTTTTCTTGGTGTTTGGCATTCTTGCTATGAACAAAACAAAGCGTTTTGTATTTCTTTTATTTAACAATTCCTTGTCCAATTTCTTGTGGACATGATTAGAGCAGGGGTGGGTTCTTGAAATTTGTCCATTATTATCTGTGTGAGTTGACAATGACATGAAGACTACCACCATACAATAATCTAAACATATTTGGTTCTGTTAATATTTTGTTAAAGTCAATAGTTTATTTCAGAAAAAGAAAATGAGATGATTATGAATTGAAAATGAAAAGAAATGACAAAAGTGTAA

Coding sequence (CDS)

ATGGAGAATGCTATTTTGAATCTTCCATCAAACCAGGAGAGTATGATCTGGCTGGTTGATTTCAAGGATTTTGACATGTCAAATATCTCATTAAAAGCAACAAAGGAGACTGCACATATTTTACAAGAACATTATCCCGAACGCCTCGGATTGGCAATTCTGTATAACGCGCCCAAGTTTTTTGAACCCTTCTGGATGGTTGCAAAGCCTTTCCTGGAGACAAAAACTGCCAACAAAGTGAAATTTGTTTACTCTGACGACGTAAACTCAAAGAGGATAATAGAGGATCTGTTCGATATGGACCAGCTGGAGTCTGCGTTTGGTGGAAACAATAATGTTGGTTTCAATATAACCAAATATGCTGAGATGATGAAAGAAGACGATAAACAGATGCGTTGTTCCTGGTCCGCGGTAAATCCTAATAGCCTAGCCTCGGAAAAAGCTCAGAGTAGCGGTCTAGTCGGTTCTCAAATCACTAACCGTTCTGAATCCTCAGAGAATGAGAGGACAGATGGTGATATTCATATAACTAGAAAAGCAGATGGCAACTGA

Protein sequence

MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKFFEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKYAEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKADGN

Homology

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.8e-16
Identity = 41/115 (35.65%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 9   PSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKFFEPFWMVA 68
           P   E   ++VD+KDF   N+ +K   E  H L +H PER+G ++  + P  F   W + 
Sbjct: 172 PKGIEQFCFIVDYKDFGSGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKII 231

Query: 69  KPFLETKTANKVKFVYSDDVNSKRIIEDLF---DMDQLESAFGGNNNVGFNITKY 121
            PFL   T +KV+F+ S  V+ KR   +L    D++ LE   GGN +  +NI +Y
Sbjct: 232 SPFLNEVTLSKVRFINSKKVDGKRTFAELLEYVDIENLEQNLGGNLDYNYNIDEY 286

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 44/140 (31.43%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDF-DMSNI-------SLKATKETAHILQEHYPERLGLA 60
           +E  I  +P+ Q+S+  L+DFKD+ D+  +        +   KE  HILQ HYPERLG A
Sbjct: 181 LERVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHYPERLGKA 240

Query: 61  ILYNAPKFFEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNN 120
           +L N P     F  +  PF++  T  K+ F   D+   K +       ++L+S +GG+  
Sbjct: 241 LLTNIPWLAWTFLKLIHPFIDPLTREKLVF---DEPFVKYV-----PKNELDSLYGGDLK 300

Query: 121 VGFNITKYAEMMKEDDKQMR 133
             +N   Y   + E  ++ R
Sbjct: 301 FKYNHDVYWPALVETAREKR 312

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-08
Identity = 54/178 (30.34%), Postives = 80/178 (44.94%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFK-DFDMSNISLKATKETAHILQEHYPERLGLAILYNAPK 60
           +E AI  +P   E++  L++FK   + SN S+   KE  +ILQ HY ERLG A++ N P 
Sbjct: 721 LECAIDLMPPGVETLALLINFKSSSNRSNPSVGQGKEVLNILQTHYCERLGRALVINIPW 780

Query: 61  FFEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITK 120
               F+ +  PF++  T  K+KF           ++     DQL+S FGG+ +  ++  K
Sbjct: 781 AVWGFFKLISPFIDPITREKLKF--------NEPLDRYVPKDQLDSNFGGSLHFEYHHEK 840

Query: 121 YAEMMKEDDKQMRC----SWSAVNPNSLASEKAQSSG---------LVGSQITNRSES 165
           Y   + E  K  R      W  +      SE     G          V   +TNRS S
Sbjct: 841 YWPQLVELCKSRRLGILEKWRKMGSKIGTSEWDLKGGEEYVELMQQYVRPSLTNRSSS 890

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.4e-05
Identity = 33/115 (28.70%), Postives = 52/115 (45.22%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNI------SLKATKETAHILQEHYPERLGLAIL 60
           ME A    P   E +  LVDFK +    I       +   +   +++Q+HYPERL   +L
Sbjct: 185 METATTVAPQGVEKITVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPERLAKCVL 244

Query: 61  YNAPKFFEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGG 110
            N P F   F  +  PFL+   A K K ++ +        E+  +  QL++ + G
Sbjct: 245 INIPWFAWAFLKMMYPFLD--PATKAKAIFDEP------FENHIEPSQLDALYNG 291

[Close]

BLAST of Cucsat.G7989.T2 vs. NCBI nr
Match: XP_031740700.1 (phosphatidylinositol transfer protein 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 365 bits (938), Expect = 6.14e-126
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF
Sbjct: 100 MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 159

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 160 FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 219

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 180
           AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA
Sbjct: 220 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 279

Query: 181 DGN 183
           DGN
Sbjct: 280 DGN 282

[Close]

BLAST of Cucsat.G7989.T2 vs. NCBI nr
Match: XP_004150523.1 (phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >KGN54617.1 hypothetical protein Csa_012269 [Cucumis sativus])

HSP 1 Score: 365 bits (938), Expect = 1.92e-125
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF
Sbjct: 133 MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 192

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 193 FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 252

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 180
           AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA
Sbjct: 253 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 312

Query: 181 DGN 183
           DGN
Sbjct: 313 DGN 315

[Close]

BLAST of Cucsat.G7989.T2 vs. NCBI nr
Match: TYK18594.1 (random slug protein 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 335 bits (858), Expect = 4.40e-113
Identity = 169/185 (91.35%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYNAPKF
Sbjct: 144 MENAILNLPSNQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNAPKF 203

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDD+NSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 204 FEPFWMVAKPFLETKTANKVKFVYSDDLNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 263

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITN-RSESSENERTDGDIHITR 180
           AEMMKEDDKQMRC WS VNPN LASEKAQSS GLV S+ +N  SESS+NERTDGD+HITR
Sbjct: 264 AEMMKEDDKQMRCFWSGVNPNGLASEKAQSSSGLVDSKTSNFSSESSKNERTDGDVHITR 323

Query: 181 KADGN 183
            ADGN
Sbjct: 324 DADGN 328

[Close]

BLAST of Cucsat.G7989.T2 vs. NCBI nr
Match: XP_008444104.1 (PREDICTED: random slug protein 5-like [Cucumis melo] >KAA0064224.1 random slug protein 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 335 bits (858), Expect = 7.02e-113
Identity = 169/185 (91.35%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYNAPKF
Sbjct: 158 MENAILNLPSNQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNAPKF 217

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDD+NSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 218 FEPFWMVAKPFLETKTANKVKFVYSDDLNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 277

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITN-RSESSENERTDGDIHITR 180
           AEMMKEDDKQMRC WS VNPN LASEKAQSS GLV S+ +N  SESS+NERTDGD+HITR
Sbjct: 278 AEMMKEDDKQMRCFWSGVNPNGLASEKAQSSSGLVDSKTSNFSSESSKNERTDGDVHITR 337

Query: 181 KADGN 183
            ADGN
Sbjct: 338 DADGN 342

[Close]

BLAST of Cucsat.G7989.T2 vs. NCBI nr
Match: XP_038899982.1 (phosphatidylinositol transfer protein 3-like isoform X2 [Benincasa hispida] >XP_038899983.1 phosphatidylinositol transfer protein 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 314 bits (804), Expect = 5.08e-105
Identity = 161/185 (87.03%), Postives = 167/185 (90.27%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLP +QESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYN PKF
Sbjct: 133 MENAILNLPPDQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNPPKF 192

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNN-VGFNITK 120
           FEPFWMVAKPFLETKT NKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNN  GFNITK
Sbjct: 193 FEPFWMVAKPFLETKTTNKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNNAGFNITK 252

Query: 121 YAEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITNRS-ESSENERTDGDIHIT 180
           YAEMMKEDDKQMRC WS VNP   ASEKAQSS GL  S+ +N S ES ENERTDGD+HIT
Sbjct: 253 YAEMMKEDDKQMRCFWSGVNPRGPASEKAQSSSGLADSKTSNSSSESPENERTDGDVHIT 312

Query: 181 RKADG 182
           R ADG
Sbjct: 313 RNADG 317

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL
Match: A0A0A0L1Z8 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G378770 PE=4 SV=1)

HSP 1 Score: 365 bits (938), Expect = 9.32e-126
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF
Sbjct: 133 MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 192

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 193 FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 252

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 180
           AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA
Sbjct: 253 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 312

Query: 181 DGN 183
           DGN
Sbjct: 313 DGN 315

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL
Match: A0A5D3D4Y6 (Random slug protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001230 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 2.13e-113
Identity = 169/185 (91.35%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYNAPKF
Sbjct: 144 MENAILNLPSNQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNAPKF 203

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDD+NSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 204 FEPFWMVAKPFLETKTANKVKFVYSDDLNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 263

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITN-RSESSENERTDGDIHITR 180
           AEMMKEDDKQMRC WS VNPN LASEKAQSS GLV S+ +N  SESS+NERTDGD+HITR
Sbjct: 264 AEMMKEDDKQMRCFWSGVNPNGLASEKAQSSSGLVDSKTSNFSSESSKNERTDGDVHITR 323

Query: 181 KADGN 183
            ADGN
Sbjct: 324 DADGN 328

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL
Match: A0A5A7VAG2 (Random slug protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G001740 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 3.40e-113
Identity = 169/185 (91.35%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYNAPKF
Sbjct: 158 MENAILNLPSNQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNAPKF 217

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDD+NSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 218 FEPFWMVAKPFLETKTANKVKFVYSDDLNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 277

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITN-RSESSENERTDGDIHITR 180
           AEMMKEDDKQMRC WS VNPN LASEKAQSS GLV S+ +N  SESS+NERTDGD+HITR
Sbjct: 278 AEMMKEDDKQMRCFWSGVNPNGLASEKAQSSSGLVDSKTSNFSSESSKNERTDGDVHITR 337

Query: 181 KADGN 183
            ADGN
Sbjct: 338 DADGN 342

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL
Match: A0A1S3BAC1 (random slug protein 5-like OS=Cucumis melo OX=3656 GN=LOC103487543 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 3.40e-113
Identity = 169/185 (91.35%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNLPSNQESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLGLAILYNAPKF
Sbjct: 158 MENAILNLPSNQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGLAILYNAPKF 217

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYSDD+NSKRIIEDLFDMDQLESAFGGNNNVGFNITKY
Sbjct: 218 FEPFWMVAKPFLETKTANKVKFVYSDDLNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 277

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSS-GLVGSQITN-RSESSENERTDGDIHITR 180
           AEMMKEDDKQMRC WS VNPN LASEKAQSS GLV S+ +N  SESS+NERTDGD+HITR
Sbjct: 278 AEMMKEDDKQMRCFWSGVNPNGLASEKAQSSSGLVDSKTSNFSSESSKNERTDGDVHITR 337

Query: 181 KADGN 183
            ADGN
Sbjct: 338 DADGN 342

[Close]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL
Match: A0A6J1FR04 (phosphatidylinositol transfer protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111446132 PE=4 SV=1)

HSP 1 Score: 275 bits (703), Expect = 3.18e-90
Identity = 140/182 (76.92%), Postives = 151/182 (82.97%), Query Frame = 0


Query: 1   MENAILNLPSNQESMIWLVDFKDFDMSNISLKATKETAHILQEHYPERLGLAILYNAPKF 60
           MENAILNL  +QESMIWL+DFKDFDMSNISLKATKETAH+LQEHYPERLG+AILYNAPKF
Sbjct: 133 MENAILNLSPDQESMIWLIDFKDFDMSNISLKATKETAHVLQEHYPERLGVAILYNAPKF 192

Query: 61  FEPFWMVAKPFLETKTANKVKFVYSDDVNSKRIIEDLFDMDQLESAFGGNNNVGFNITKY 120
           FEPFWMVAKPFLETKTANKVKFVYS+DVNSKRIIE LF+MDQLESAFGGNN+V FNITKY
Sbjct: 193 FEPFWMVAKPFLETKTANKVKFVYSNDVNSKRIIESLFNMDQLESAFGGNNHVSFNITKY 252

Query: 121 AEMMKEDDKQMRCSWSAVNPNSLASEKAQSSGLVGSQITNRSESSENERTDGDIHITRKA 180
            EMMKEDDK+MRC WS V+P S  +       LV    T+      N RTD D HITR A
Sbjct: 253 GEMMKEDDKRMRCFWSGVDPTSRCA-------LVTDSKTS------NSRTDDDAHITRGA 301

Query: 181 DG 182
           DG
Sbjct: 313 DG 301

The following BLAST results are available for this feature:

BLAST of Cucsat.G7989.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55CU8	6.8e-16	35.65	Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=...	[more]
P53860	1.2e-09	31.43	Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ...	[more]
Q9UU99	1.8e-08	30.34	CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra...	[more]
P53844	2.4e-05	28.70	Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ...	[more]

BLAST of Cucsat.G7989.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740700.1	6.14e-126	100.00	phosphatidylinositol transfer protein 3 isoform X2 [Cucumis sativus]	[more]
XP_004150523.1	1.92e-125	100.00	phosphatidylinositol transfer protein 3 isoform X1 [Cucumis sativus] >KGN54617.1...	[more]
TYK18594.1	4.40e-113	91.35	random slug protein 5-like [Cucumis melo var. makuwa]	[more]
XP_008444104.1	7.02e-113	91.35	PREDICTED: random slug protein 5-like [Cucumis melo] >KAA0064224.1 random slug p...	[more]
XP_038899982.1	5.08e-105	87.03	phosphatidylinositol transfer protein 3-like isoform X2 [Benincasa hispida] >XP_...	[more]

BLAST of Cucsat.G7989.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1Z8	9.32e-126	100.00	CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G378770 P...	[more]
A0A5D3D4Y6	2.13e-113	91.35	Random slug protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7VAG2	3.40e-113	91.35	Random slug protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BAC1	3.40e-113	91.35	random slug protein 5-like OS=Cucumis melo OX=3656 GN=LOC103487543 PE=4 SV=1	[more]
A0A6J1FR04	3.18e-90	76.92	phosphatidylinositol transfer protein 3-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001251	CRAL-TRIO lipid binding domain	SMART	SM00516	sec14_4	coord: 1..112 e-value: 8.6E-11 score: 51.9
IPR001251	CRAL-TRIO lipid binding domain	PFAM	PF00650	CRAL_TRIO	coord: 1..110 e-value: 4.7E-24 score: 84.9
IPR001251	CRAL-TRIO lipid binding domain	PROSITE	PS50191	CRAL_TRIO	coord: 1..115 score: 15.372032
IPR001251	CRAL-TRIO lipid binding domain	CDD	cd00170	SEC14	coord: 1..110 e-value: 8.07357E-30 score: 105.545
IPR036865	CRAL-TRIO lipid binding domain superfamily	GENE3D	3.40.525.10		coord: 1..152 e-value: 6.1E-38 score: 132.6
IPR036865	CRAL-TRIO lipid binding domain superfamily	SUPERFAMILY	52087	CRAL/TRIO domain	coord: 1..130
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 164..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 144..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 144..163
None	No IPR available	PANTHER	PTHR45824	GH16843P	coord: 1..171
None	No IPR available	PANTHER	PTHR45824:SF24	CRAL-TRIO LIPID BINDING DOMAIN-CONTAINING PROTEIN-RELATED	coord: 1..171

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G7989	Cucsat.G7989	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7989.T2.E1	Cucsat.G7989.T2.E1	exon
Cucsat.G7989.T2.E2	Cucsat.G7989.T2.E2	exon
Cucsat.G7989.T2.E3	Cucsat.G7989.T2.E3	exon
Cucsat.G7989.T2.E4	Cucsat.G7989.T2.E4	exon
Cucsat.G7989.T2.E5	Cucsat.G7989.T2.E5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7989.T2.C2	Cucsat.G7989.T2.C2	CDS
Cucsat.G7989.T2.C1	Cucsat.G7989.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G7989.T2	Cucsat.G7989.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0120009	intermembrane lipid transfer
biological_process	GO:0015914	phospholipid transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0008526	phosphatidylinositol transfer activity