Cucsat.G7371.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G7371.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCholine monooxygenase, chloroplastic
Locationctg1528: 308340 .. 311655 (+)
RNA-Seq ExpressionCucsat.G7371.T1
SyntenyCucsat.G7371.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAAAAAGAAAATATGTGTGTTAGTTAGCCACCAGAACGGTAGAATAAAACGAGTTGTTATTTGAAGAAGAAGCTTGAGGACTGTTGCAATGGCGATGTTGACGAAGCATATCCAAGTTCACTTCTTCCAACTTCCTTCCATTTCCTTCAATTTCCATTCCTGTAATCATCGTTCTCCCCCACGGATCTCCGCAGCTTTATCCTTTCGAAACCCTGATTCTCGACTCATTGAAGCTCGGAAACTCGTTGATGATTTCGACCCTCAAATTCCATTGGAGAAGGCCCTCACTCCACCCTCCTCCTGGTATATAGACCCTTCATTTTTTGCTCTCGAGCTCAATCATGTCTTCTACAGAGGATGGCAGGCTGTAGGTTCGCTTCCATCTCTAAATTTTTTGTGTTTTTTTTTTTACTCTATTTATGCCATGTATGTTTAGAATCACTTTGAAATATGTTTTTAATGATCGAAAATCAGTTTGGCTATATGAAACTTGTATTTAAAGTGTAGAATTAAATACTAAACTAATTTGGAGGGTTTAAAAATGTGTTTCCAAAATATTTTGAAGATGCCAAAAGTGACATTAGGAATTTTAAAATCACTCTGAAAATGCACTTAATTGTAGCTTCATCGAGAGGAGATTAATTGCTATATTTCAATGTGGCTGCAATTTGCGATCTATGATTATAATGGAAATTTCTTCCATTAACTGAGAACTCGGAAGTTCTTGGTCTTTGACAGAATACAAAGCAGGATAATCCCACTGAATTTGTGTACTCTTCTTTGTTTTACAGCTAATGCAATCTGCTCCTTGTTTTTCCCCTTTAGATTATAACTTTGACAATATTAAGGCATGATCATCAACTTTTTGTGCAATTGGCTTGTCAGGATATGTTGAACAGTTAAAAGATGCCCATGACTTTTTCACAGGCAGGTGTGATTAATTGTCACTTACCATTTGTTGCTGATAGTATAAACACTAGAGAGCCGTAATAAAATTATTTGTATTACCTGTATGTTGAATATATTTTTCACAGGGTAATTGAGCAAATATGGAGTTGAATCACTTGACATTTTAATGCCCTGAGTCTAAGTAATGGGATGCCAGAGATGTGTGTTCTTCCTCACCATGGCTTGTCCTTGAAATTTGTTTGCTCTGTCTCCTTGACTATGGCAGGTTGGGAAATGTAGAGTATGTGGTATGCAAAGATAATAACAGAAAGGTTCGTGCATTTCACAATGTTTGTCGCCATCATGCCTCACTTCTTGCGTCTGGATGTGGGAAGAAGTCGTGCTTTGTATGCCCATATCATGTGAGTTCACTTGTATATCTCCTTATCGGTTCCCTGGAAAGTTTTTAGTTTTCTATTTCCTAGTTATTTCTTGACGGCCAGGTTAAGGTTTTAGTCCATAACTTTACCATCATCGTGTAAGGAACAGTGATTTTCATTGAAGAAAAAAGAGATCATTTGTAAAGGTGAAAAATGGAAATCTCCAAGCTATGCTCTGATCTTTTGAAAAATATGTAAAATGTAACTAGGATGGAATATTATGCGATATTACGAGGTGCATCAATATGGGTTGTTACATATTTTGTGCAGGGATGGACATACGGGTTGGATGGAATACTGCTTAAGGCGACTAGAATAGATGGGATACAGAACTTTGATGAAAATGTATGTAATTATATCATTTTGATACCAGTTTCTACAATCATTTTAATGTAATATTTCTGAAAGGCACTTAGTTTATTTAATGCTGGAGATTAGGATTTTGGGCTTGTACCATTGCCAGTAGCTACGTGGGGGCCTTTCGTTCTTCTCAATTTGGATGGAAAATTATCATCTAAGCCAGATGTTGATGAAAATAAAGTGGCACGTGAATGGCTTGGAACATGTGCAGATGTGCTGAGGCTAAACGGAGTTGATGCTTCCCTAAGTTATGTCTGTCGACGTGAATACACTATTGAATGTAACTGGAAGGTATAGTCCTTGCATCCTAATTCTTAAAAAATGGTCATGTACTGTTACCACAATTCTTGTGGGTTTTGCTAATACGTTTCATTGGTTTTTTGCAGGTTTTTTGTGACAACTATTTAGATGGAGGATATCACGTTCCATATGCACATAAAGGGCTTGCATCTAATCTCAAGCTTGAGTCTTATTCTACAGAAGTATGTTGAATGTTTATTTACCGTGTAGTCAATCATTCACAGTAAATTATCAATGTTATGTGACTATAACTTGTGATGTAGCTATTTGAAACTGTTAGCATTCAAAGTTGTAAGGGTGGGGGAGAATCAAAAGGTGATGATTATGGTCGACTTGGATCAGAAGCACTCTATGCTTTTGTATACCCAAATTTCATGATAAATAGGTAGTTCCTGGCCATATCAATATACCTAGCTCTTTGGCTCTTTATGTTTTGAGATGTTAAGTCGTGATGGTCGTCTTATTATTTTTCGGCTTGTGTAATAGGTATGGACCTTGGATGGACACTAATCTAGTACTCCCACTTGGGCCTCGAAAATGTCTGGTGGTTTTTGATTATTTTCTTGAAGCTTCTTTTAAGGTAATTTCTGGTCCTTTGGCGTAATGGATATGTGATGTGCACAAGTTCTCACTTGTGTTCTTGATTTATTAAAAGGACTGCTCGTGTAACGCGAAAACTCAGCAAAAACCGAGCTTTTGTTGTATTGAAGTCTTTGGTTCATTTGGGTTCTTACCTTTTTTCTTCCTTTTATTTTTTATTTTTTTGAAATTAACTTTTTCGCTTCCTGATTTCATGTTCAGAATGATGACTCCTTTATACAACTAAGTTTAGAAGACAGTGAAAGCGTGCAGGTATTACGAACAAATCCACCCCTTTCCATTTTCATGTGTGCTGTGTCTGTTCTTTTTCTCTTACACATTGGTCCAAATAATATTCAGAATGAAGACATTATTCTGTGTGAAGGAGTTCAAAAGGGTCTCGAGTCACCAGCTTACAAGTTTGGCCGATATGCACCTTCGGTCGAGAATGCCATGCACCATTTCCATCGTCTTCTTCATTGTAACCTCACAAAATAAAAATAATTGAAAGAATGCCATGCACCATTTGCAAGATATTCTTTTAGGTTTTCATAGTTATCTTGGAAATGGTCTGAAATAACTTGCTTTTGTTACAGCACCTTTAGTTGTTGAAGCTGTGATATATACCAAATATGATAATTTTATTAATGTTTAAAAGTTGTGAATACAGTAAGGCAAGTAATTTCACAGCCCATTTGGATGATTTCTTTTATCTTCTCGAGTGACTAAATGTGATTCTTGATGAT

Coding sequence (CDS)

ATGGCGATGTTGACGAAGCATATCCAAGTTCACTTCTTCCAACTTCCTTCCATTTCCTTCAATTTCCATTCCTGTAATCATCGTTCTCCCCCACGGATCTCCGCAGCTTTATCCTTTCGAAACCCTGATTCTCGACTCATTGAAGCTCGGAAACTCGTTGATGATTTCGACCCTCAAATTCCATTGGAGAAGGCCCTCACTCCACCCTCCTCCTGGTATATAGACCCTTCATTTTTTGCTCTCGAGCTCAATCATGTCTTCTACAGAGGATGGCAGGCTGTAGGATATGTTGAACAGTTAAAAGATGCCCATGACTTTTTCACAGGCAGGTTGGGAAATGTAGAGTATGTGGTATGCAAAGATAATAACAGAAAGGTTCGTGCATTTCACAATGTTTGTCGCCATCATGCCTCACTTCTTGCGTCTGGATGTGGGAAGAAGTCGTGCTTTGTATGCCCATATCATGGATGGACATACGGGTTGGATGGAATACTGCTTAAGGCGACTAGAATAGATGGGATACAGAACTTTGATGAAAATGATTTTGGGCTTGTACCATTGCCAGTAGCTACGTGGGGGCCTTTCGTTCTTCTCAATTTGGATGGAAAATTATCATCTAAGCCAGATGTTGATGAAAATAAAGTGGCACGTGAATGGCTTGGAACATGTGCAGATGTGCTGAGGCTAAACGGAGTTGATGCTTCCCTAAGTTATGTCTGTCGACGTGAATACACTATTGAATGTAACTGGAAGGTTTTTTGTGACAACTATTTAGATGGAGGATATCACGTTCCATATGCACATAAAGGGCTTGCATCTAATCTCAAGCTTGAGTCTTATTCTACAGAACTATTTGAAACTGTTAGCATTCAAAGTTGTAAGGGTGGGGGAGAATCAAAAGGTGATGATTATGGTCGACTTGGATCAGAAGCACTCTATGCTTTTGTATACCCAAATTTCATGATAAATAGGTATGGACCTTGGATGGACACTAATCTAGTACTCCCACTTGGGCCTCGAAAATGTCTGGTGGTTTTTGATTATTTTCTTGAAGCTTCTTTTAAGAATGATGACTCCTTTATACAACTAAGTTTAGAAGACAGTGAAAGCGTGCAGAATGAAGACATTATTCTGTGTGAAGGAGTTCAAAAGGGTCTCGAGTCACCAGCTTACAAGTTTGGCCGATATGCACCTTCGGTCGAGAATGCCATGCACCATTTCCATCGTCTTCTTCATTGTAACCTCACAAAATAA

Protein sequence

MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK
Homology
[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match: Q9SZR0 (Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g29890 PE=2 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 2.7e-153
Identity = 260/399 (65.16%), Postives = 306/399 (76.69%), Query Frame = 0 

Query: 20  FNFHSCNHRSPPRISAALSFRNPDS--RLIEARKLVDDFDPQIPLEKALTPPSSWYIDPS 79
           FN HS    S  + S    F NP     + +  KLV +FDP+IPLE+A TPPSSWY DP 
Sbjct: 24  FNSHSEFGVSISKFSRR-RFHNPTRVFAVSDISKLVTEFDPKIPLERASTPPSSWYTDPQ 83

Query: 80  FFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHA 139
           F++ EL+ VFY GWQAVGY +Q+K++ DFFTGRLG+V++VVC+D N K+ AFHNVC HHA
Sbjct: 84  FYSFELDRVFYGGWQAVGYSDQIKESRDFFTGRLGDVDFVVCRDENGKIHAFHNVCSHHA 143

Query: 140 SLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVL 199
           S+LASG G+KSCFVC YHGWTY L G L+KATR+ GIQNF  ++ GL PL VA WGPFVL
Sbjct: 144 SILASGNGRKSCFVCLYHGWTYSLSGSLVKATRMSGIQNFSLSEMGLKPLRVAVWGPFVL 203

Query: 200 LNLDGKLSSKPDVDENK-VAREWLGTCADVLRLNGVDASLSYVCRREYTIECNWKVFCDN 259
           L +    S K +V+ ++ VA EWLGT    L   GVD+ LSY+CRREYTI+CNWKVFCDN
Sbjct: 204 LKVTAATSRKGEVETDELVASEWLGTSVGRLSQGGVDSPLSYICRREYTIDCNWKVFCDN 263

Query: 260 YLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESKGDDYGRLGSEALYAFV 319
           YLDGGYHVPYAHKGL S L LE+YST +FE VSIQ C GG +   D + RLGSEALYAFV
Sbjct: 264 YLDGGYHVPYAHKGLMSGLDLETYSTTIFEKVSIQECGGGSKVGEDGFDRLGSEALYAFV 323

Query: 320 YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDI 379
           YPNFMINRYGPWMDTNLVLPLGPRKC VVFDYFL+ S K+D++FI+ SLE+S+ VQ ED+
Sbjct: 324 YPNFMINRYGPWMDTNLVLPLGPRKCKVVFDYFLDPSLKDDEAFIKRSLEESDRVQMEDV 383

Query: 380 ILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNL 416
           +LCE VQ+GLES AY  GRYA  VE  MHHFH LLH NL
Sbjct: 384 MLCESVQRGLESQAYDKGRYA-LVEKPMHHFHCLLHHNL 420

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match: Q93XE1 (Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 9.4e-122
Identity = 195/385 (50.65%), Postives = 266/385 (69.09%), Query Frame = 0 

Query: 31  PRISAALSFRNPDSRLIEARKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRG 90
           P I ++++  N  +     ++++ +FDP++P E   TPPS+WY DPS ++ EL+ +F +G
Sbjct: 63  PVIPSSINSNNITTTTPNIKRIIHEFDPKVPAEDGFTPPSTWYTDPSLYSHELDRIFSKG 122

Query: 91  WQAVGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCF 150
           WQ  GY +Q+K+ + +FTG LGNVEY+VC+D   KV AFHNVC H AS+LA G GKKSCF
Sbjct: 123 WQVAGYSDQIKEPNQYFTGSLGNVEYLVCRDGQGKVHAFHNVCTHRASILACGTGKKSCF 182

Query: 151 VCPYHGWTYGLDGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDV 210
           VCPYHGW +GLDG L+KAT+ +  Q FD  + GLV L VA WGPFVL++LD   S   + 
Sbjct: 183 VCPYHGWVFGLDGSLMKATKTEN-QVFDPKELGLVTLKVAIWGPFVLISLDRSGSEGTE- 242

Query: 211 DENKVAREWLGTCADVLRLNGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKG 270
               V +EW+G+CA+ ++ +  D SL ++ R E+ +E NWKVFCDNYLD  YHVPYAHK 
Sbjct: 243 ---DVGKEWIGSCAEEVKKHAFDPSLQFINRSEFPMESNWKVFCDNYLDSAYHVPYAHKY 302

Query: 271 LASNLKLESYSTELFETVSIQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMD 330
            A+ L  ++Y T+L E V IQ       +K + + RLGSEA YAF+YPNF + RYGPWM 
Sbjct: 303 YAAELDFDTYKTDLLEKVVIQRV-ASSSNKPNGFDRLGSEAFYAFIYPNFAVERYGPWMT 362

Query: 331 TNLVLPLGPRKCLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPA 390
           T  + PLGPRKC +V DY+LE +  ND  +I+ S+  +++VQ ED++LCE VQ+GLE+PA
Sbjct: 363 TMHIGPLGPRKCKLVVDYYLENAMMNDKPYIEKSIMINDNVQKEDVVLCESVQRGLETPA 422

Query: 391 YKFGRYAPSVENAMHHFHRLLHCNL 416
           Y+ GRY   +E  +HHFH  LH  L
Sbjct: 423 YRSGRYVMPIEKGIHHFHCWLHQTL 441

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match: Q9LKN0 (Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.3e-120
Identity = 188/363 (51.79%), Postives = 257/363 (70.80%), Query Frame = 0 

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           + LV DFDP +P E ALTPPSSWY +P+F+A EL+ +FY+GWQ  GY +Q+K+A+ +FTG
Sbjct: 80  QSLVKDFDPLVPAEDALTPPSSWYTEPAFYAHELDRIFYKGWQVAGYSDQVKEANQYFTG 139

Query: 110 RLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKAT 169
            LGNVEY+VC+D   KV AFHNVC H AS+LA G GKKSCFVCPYHGW YG++G L KA+
Sbjct: 140 TLGNVEYLVCRDGEGKVHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGMNGSLTKAS 199

Query: 170 RIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRL 229
           +    Q+ + ++ GLVPL VA WGPF+L++LD     +   +   V  EWLG+CA+ ++ 
Sbjct: 200 KATPEQSLNPDELGLVPLKVAVWGPFILISLD-----RSSREVGDVGSEWLGSCAEDVKA 259

Query: 230 NGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVS 289
           +  D +L ++ R E+ IE NWK+F DNYLD  YHVPYAHK  A+ L  ++Y T++   V+
Sbjct: 260 HAFDPNLQFINRSEFPIESNWKIFSDNYLDSSYHVPYAHKYYATELDFDTYQTDMVGNVT 319

Query: 290 IQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYF 349
           IQ   G   +  + + RLG++A YAF YPNF + RYGPWM T  ++PLGPRKC +V DY+
Sbjct: 320 IQRVAG---TSNNGFNRLGTQAFYAFAYPNFAVERYGPWMTTMHIVPLGPRKCKLVVDYY 379

Query: 350 LEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHR 409
           +E S  +D  +I+  +  +++VQ ED++LCE VQKGLE+PAY+ GRY   +E  +HHFH 
Sbjct: 380 IEKSKLDDKDYIEKGIAINDNVQKEDVVLCESVQKGLETPAYRSGRYVMPIEKGIHHFHC 434

Query: 410 LLH 413
            LH
Sbjct: 440 WLH 434

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match: O22553 (Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.8e-120
Identity = 190/363 (52.34%), Postives = 252/363 (69.42%), Query Frame = 0 

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           R LV +FDP+IP E ALTPPS+WY +P+F++ EL  +FY+GWQ  GY EQ+K+ + +FTG
Sbjct: 86  RSLVHEFDPEIPPEDALTPPSTWYTEPAFYSHELERIFYKGWQVAGYSEQVKEKNQYFTG 145

Query: 110 RLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKAT 169
            LGNVEY+V +D   ++ AFHNVC H AS+LA G GKKSCFVCPYHGW YGLDG L KA+
Sbjct: 146 SLGNVEYLVSRDGQGELHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGLDGSLAKAS 205

Query: 170 RIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRL 229
           +    QN D  + GL PL VA WGPF+L++LD  L +  D     V  EW+G  A+ ++ 
Sbjct: 206 KATETQNLDPKELGLAPLKVAEWGPFILISLDRSLDANAD-----VGTEWIGKSAEDVKA 265

Query: 230 NGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVS 289
           +  D +L +  R E+ +ECNWKVFCDNYLD  YHVPYAHK  A+ L  ++Y+TE+ E   
Sbjct: 266 HAFDPNLKFTHRSEFPMECNWKVFCDNYLDSSYHVPYAHKYYAAELDFDTYNTEMIEKCV 325

Query: 290 IQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYF 349
           IQ   G   +K D + RLG+EA YAF+YPNF + RYG WM T  V+P+G RKC +V DY+
Sbjct: 326 IQRV-GSSSNKPDGFDRLGTEAFYAFIYPNFAVERYGTWMTTMHVVPMGQRKCKLVVDYY 385

Query: 350 LEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHR 409
           LE +  +D ++I   +  +++VQ ED +LCE VQ+GLE+PAY+ GRY   +E  +HHFH 
Sbjct: 386 LEKAMLDDKAYIDKGIAINDNVQKEDKVLCESVQRGLETPAYRSGRYVMPIEKGIHHFHC 442

Query: 410 LLH 413
            LH
Sbjct: 446 WLH 442

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match: O04121 (Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 5.7e-119
Identity = 189/359 (52.65%), Postives = 250/359 (69.64%), Query Frame = 0 

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           + LV +FDPQIP E A TPPSSWY +P+F++ EL  +FY+GWQ  G  +Q+K+ + +FTG
Sbjct: 79  QSLVHEFDPQIPPEDAHTPPSSWYTEPAFYSHELERIFYKGWQVAGISDQIKEPNQYFTG 138

Query: 110 RLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKAT 169
            LGNVEY+V +D   KV AFHNVC H AS+LA G GKKSCFVCPYHGW YG+DG L KA+
Sbjct: 139 SLGNVEYLVSRDGEGKVHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGMDGSLAKAS 198

Query: 170 RIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRL 229
           +    QN D  + GLVPL VA WGPFVL++LD  L    D     V  EWLGT A+ ++ 
Sbjct: 199 KAKPEQNLDPKELGLVPLKVAVWGPFVLISLDRSLEEGGD-----VGTEWLGTSAEDVKA 258

Query: 230 NGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVS 289
           +  D SL ++ R E+ +E NWK+F DNYLD  YHVPYAHK  A+ L  ++Y T++ E V+
Sbjct: 259 HAFDPSLQFIHRSEFPMESNWKIFSDNYLDSSYHVPYAHKYYATELNFDTYDTQMIENVT 318

Query: 290 IQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYF 349
           IQ  + G  +K D + R+G +A YAF YPNF + RYGPWM T  + PLGPRKC +V DY+
Sbjct: 319 IQRVE-GSSNKPDGFDRVGIQAFYAFAYPNFAVERYGPWMTTMHIHPLGPRKCKLVVDYY 378

Query: 350 LEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFH 409
           +E S  +D  +I+  +  +++VQ ED++LCE VQ+GLE+PAY+ GRY   +E  +HHFH
Sbjct: 379 IENSMLDDKDYIEKGIAINDNVQREDVVLCESVQRGLETPAYRSGRYVMPIEKGIHHFH 431

[Close]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match: XP_004139149.1 (choline monooxygenase, chloroplastic isoform X1 [Cucumis sativus] >KGN66608.1 hypothetical protein Csa_007257 [Cucumis sativus])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 414/417 (99.28%), Postives = 415/417 (99.52%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MAMLTKHIQVHFFQLPS SFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI
Sbjct: 1   MAMLTKHIQVHFFQLPSTSFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDD GRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF
Sbjct: 301 GDDIGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK
Sbjct: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match: XP_008443631.1 (PREDICTED: choline monooxygenase, chloroplastic isoform X2 [Cucumis melo] >KAA0060178.1 choline monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 838 bits (2164), Expect = 1.77e-306
Identity = 395/417 (94.72%), Postives = 406/417 (97.36%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGLDGILLKATRI+GIQNF+EN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVA WGPFVLLNLDGKLSSKPDVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVAMWGPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF
Sbjct: 301 GDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHRNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match: TYK04233.1 (choline monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 833 bits (2152), Expect = 1.20e-304
Identity = 393/417 (94.24%), Postives = 405/417 (97.12%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGLDGILLKATRI+GIQNF+EN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVA WGPFVLLNLDGKLSSK DVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVAMWGPFVLLNLDGKLSSKVDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLE+SFKNDDSF
Sbjct: 301 GDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLESSFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHRNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match: XP_016899776.1 (PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 826 bits (2133), Expect = 1.94e-301
Identity = 394/436 (90.37%), Postives = 406/436 (93.12%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAV-------------------GYVEQLK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQA+                   GYVEQLK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAIITLTILRHGSSTFCAIGLSGYVEQLK 120

Query: 121 DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGL 180
           DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGL
Sbjct: 121 DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGL 180

Query: 181 DGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLG 240
           DGILLKATRI+GIQNF+ENDFGLVPLPVA WGPFVLLNLDGKLSSKPDVDE+KVAREWLG
Sbjct: 181 DGILLKATRINGIQNFNENDFGLVPLPVAMWGPFVLLNLDGKLSSKPDVDEDKVAREWLG 240

Query: 241 TCADVLRLNGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYS 300
           TCADVLRLNGVDASLSYVCRREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYS
Sbjct: 241 TCADVLRLNGVDASLSYVCRREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYS 300

Query: 301 TELFETVSIQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRK 360
           TELFETVSIQSCKGGGESKGDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRK
Sbjct: 301 TELFETVSIQSCKGGGESKGDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRK 360

Query: 361 CLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE 417
           CLVVFDYFLEASFKNDDSFIQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE
Sbjct: 361 CLVVFDYFLEASFKNDDSFIQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE 420

[Close]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match: XP_038880722.1 (choline monooxygenase, chloroplastic [Benincasa hispida])

HSP 1 Score: 801 bits (2068), Expect = 7.61e-292
Identity = 375/417 (89.93%), Postives = 395/417 (94.72%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHI  HFFQ PSISFN HSCNHRSP RISAALSFRN DS  IEA+KLVD+FDP+I
Sbjct: 1   MATLTKHIHTHFFQPPSISFNLHSCNHRSPSRISAALSFRNSDSHFIEAQKLVDEFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKA+TPPSSWYIDPSF+ALEL+ VFYRGWQAVGYVEQLKD HDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKAVTPPSSWYIDPSFYALELDRVFYRGWQAVGYVEQLKDPHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLASGCGK+SCFVCPYHGWTYGLDG+LLKATRI+GIQNFDEN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLASGCGKRSCFVCPYHGWTYGLDGVLLKATRINGIQNFDEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           +FGL+PLPVATWGPFVLLNLDGKLSS+ DVDE+KV  EWLG+CAD+L LNGVDASLSYVC
Sbjct: 181 NFGLIPLPVATWGPFVLLNLDGKLSSELDVDEDKVPGEWLGSCADLLSLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKL+SYSTE+FETVSIQSCKGGGE+K
Sbjct: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLDSYSTEIFETVSIQSCKGGGEAK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           G+DYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLG RKCLVVFDYFLEASFKND  F
Sbjct: 301 GNDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGARKCLVVFDYFLEASFKNDGPF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQ EDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQRSLEDSESVQKEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHLNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match: A0A0A0LXX3 (Choline monooxygenase, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_1G641020 PE=3 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 414/417 (99.28%), Postives = 415/417 (99.52%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MAMLTKHIQVHFFQLPS SFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI
Sbjct: 1   MAMLTKHIQVHFFQLPSTSFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDD GRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF
Sbjct: 301 GDDIGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK
Sbjct: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match: A0A5A7UY48 (Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold542G00540 PE=3 SV=1)

HSP 1 Score: 838 bits (2164), Expect = 8.59e-307
Identity = 395/417 (94.72%), Postives = 406/417 (97.36%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGLDGILLKATRI+GIQNF+EN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVA WGPFVLLNLDGKLSSKPDVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVAMWGPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF
Sbjct: 301 GDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHRNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match: A0A1S3B9A0 (Choline monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487183 PE=3 SV=1)

HSP 1 Score: 838 bits (2164), Expect = 8.59e-307
Identity = 395/417 (94.72%), Postives = 406/417 (97.36%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGLDGILLKATRI+GIQNF+EN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVA WGPFVLLNLDGKLSSKPDVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVAMWGPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF
Sbjct: 301 GDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHRNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match: A0A5D3BWX2 (Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold758G00070 PE=3 SV=1)

HSP 1 Score: 833 bits (2152), Expect = 5.80e-305
Identity = 393/417 (94.24%), Postives = 405/417 (97.12%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCK 120

Query: 121 DNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDEN 180
           DNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGLDGILLKATRI+GIQNF+EN
Sbjct: 121 DNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNEN 180

Query: 181 DFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVC 240
           DFGLVPLPVA WGPFVLLNLDGKLSSK DVDE+KVAREWLGTCADVLRLNGVDASLSYVC
Sbjct: 181 DFGLVPLPVAMWGPFVLLNLDGKLSSKVDVDEDKVAREWLGTCADVLRLNGVDASLSYVC 240

Query: 241 RREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESK 300
           RREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYSTELFETVSIQSCKGGGESK
Sbjct: 241 RREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYSTELFETVSIQSCKGGGESK 300

Query: 301 GDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSF 360
           GDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLE+SFKNDDSF
Sbjct: 301 GDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLESSFKNDDSF 360

Query: 361 IQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK 417
           IQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLH NLTK
Sbjct: 361 IQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHRNLTK 417

[Close]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match: A0A1S4DUW5 (choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487183 PE=4 SV=1)

HSP 1 Score: 826 bits (2133), Expect = 9.39e-302
Identity = 394/436 (90.37%), Postives = 406/436 (93.12%), Query Frame = 0 

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAV-------------------GYVEQLK 120
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQA+                   GYVEQLK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAIITLTILRHGSSTFCAIGLSGYVEQLK 120

Query: 121 DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGL 180
           DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLA+GCGKKSCFVCPYHGWTYGL
Sbjct: 121 DAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLATGCGKKSCFVCPYHGWTYGL 180

Query: 181 DGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLG 240
           DGILLKATRI+GIQNF+ENDFGLVPLPVA WGPFVLLNLDGKLSSKPDVDE+KVAREWLG
Sbjct: 181 DGILLKATRINGIQNFNENDFGLVPLPVAMWGPFVLLNLDGKLSSKPDVDEDKVAREWLG 240

Query: 241 TCADVLRLNGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYS 300
           TCADVLRLNGVDASLSYVCRREYTI+CNWKVFCDNYLDGGYHVPYAHKGLASNL LESYS
Sbjct: 241 TCADVLRLNGVDASLSYVCRREYTIDCNWKVFCDNYLDGGYHVPYAHKGLASNLNLESYS 300

Query: 301 TELFETVSIQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRK 360
           TELFETVSIQSCKGGGESKGDDYGRLG EALYAF+YPNFMINRYGPWMDTNLVLPLGPRK
Sbjct: 301 TELFETVSIQSCKGGGESKGDDYGRLGPEALYAFIYPNFMINRYGPWMDTNLVLPLGPRK 360

Query: 361 CLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE 417
           CLVVFDYFLEASFKNDDSFIQ SLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE
Sbjct: 361 CLVVFDYFLEASFKNDDSFIQQSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVE 420

The following BLAST results are available for this feature:
BLAST of Cucsat.G7371.T1 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SZR02.7e-15365.16Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g2989... [more]
Q93XE19.4e-12250.65Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2... [more]
Q9LKN02.3e-12051.79Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 ... [more]
O225536.8e-12052.34Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1[more]
O041215.7e-11952.65Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV... [more]
BLAST of Cucsat.G7371.T1 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004139149.10.099.28choline monooxygenase, chloroplastic isoform X1 [Cucumis sativus] >KGN66608.1 hy... [more]
XP_008443631.11.77e-30694.72PREDICTED: choline monooxygenase, chloroplastic isoform X2 [Cucumis melo] >KAA00... [more]
TYK04233.11.20e-30494.24choline monooxygenase [Cucumis melo var. makuwa][more]
XP_016899776.11.94e-30190.37PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo][more]
XP_038880722.17.61e-29289.93choline monooxygenase, chloroplastic [Benincasa hispida][more]
BLAST of Cucsat.G7371.T1 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LXX30.099.28Choline monooxygenase, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_1G641020 ... [more]
A0A5A7UY488.59e-30794.72Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B9A08.59e-30794.72Choline monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487183 PE=... [more]
A0A5D3BWX25.80e-30594.24Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S4DUW59.39e-30290.37choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001663Aromatic-ring-hydroxylating dioxygenase, alpha subunitPRINTSPR00090RNGDIOXGNASEcoord: 234..256
score: 23.1
coord: 75..100
score: 29.73
coord: 146..166
score: 44.74
coord: 105..120
score: 26.69
IPR015879Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domainPFAMPF00848Ring_hydroxyl_Acoord: 240..278
e-value: 6.4E-9
score: 36.1
coord: 311..409
e-value: 2.5E-15
score: 57.0
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilyGENE3D2.102.10.10coord: 88..207
e-value: 1.5E-30
score: 107.2
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 52..201
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 91..168
e-value: 1.7E-15
score: 56.7
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROSITEPS51296RIESKEcoord: 91..198
score: 26.232903
NoneNo IPR availableGENE3D3.90.380.10coord: 292..417
e-value: 3.1E-25
score: 91.1
coord: 209..291
e-value: 9.6E-13
score: 50.1
NoneNo IPR availablePANTHERPTHR43756:SF5CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 48..417
NoneNo IPR availableCDDcd08883RHO_alpha_C_CMO-likecoord: 239..416
e-value: 6.69853E-92
score: 272.682
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 218..411
IPR044637Aromatic-ring-hydroxylating dioxygenasePANTHERPTHR43756CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 48..417

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G7371Cucsat.G7371gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7371.T1.E1Cucsat.G7371.T1.E1exon
Cucsat.G7371.T1.E2Cucsat.G7371.T1.E2exon
Cucsat.G7371.T1.E3Cucsat.G7371.T1.E3exon
Cucsat.G7371.T1.E4Cucsat.G7371.T1.E4exon
Cucsat.G7371.T1.E5Cucsat.G7371.T1.E5exon
Cucsat.G7371.T1.E6Cucsat.G7371.T1.E6exon
Cucsat.G7371.T1.E7Cucsat.G7371.T1.E7exon
Cucsat.G7371.T1.E8Cucsat.G7371.T1.E8exon
Cucsat.G7371.T1.E9Cucsat.G7371.T1.E9exon
Cucsat.G7371.T1.E10Cucsat.G7371.T1.E10exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7371.T1.C1Cucsat.G7371.T1.C1CDS
Cucsat.G7371.T1.C2Cucsat.G7371.T1.C2CDS
Cucsat.G7371.T1.C3Cucsat.G7371.T1.C3CDS
Cucsat.G7371.T1.C4Cucsat.G7371.T1.C4CDS
Cucsat.G7371.T1.C5Cucsat.G7371.T1.C5CDS
Cucsat.G7371.T1.C6Cucsat.G7371.T1.C6CDS
Cucsat.G7371.T1.C7Cucsat.G7371.T1.C7CDS
Cucsat.G7371.T1.C8Cucsat.G7371.T1.C8CDS
Cucsat.G7371.T1.C9Cucsat.G7371.T1.C9CDS
Cucsat.G7371.T1.C10Cucsat.G7371.T1.C10CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G7371.T1Cucsat.G7371.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0019285 glycine betaine biosynthetic process from choline
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
cellular_component GO:0031967 organelle envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0019133 choline monooxygenase activity