Cucsat.G7371 (gene) Cucumber (B10) v3

Overview
NameCucsat.G7371
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCholine monooxygenase, chloroplastic
Locationctg1528: 308395 .. 311655 (+)
RNA-Seq ExpressionCucsat.G7371
SyntenyCucsat.G7371
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAAAAAGAAAATATGTGTGTTAGTTAGCCACCAGAACGGTAGAATAAAACGAGTTGTTATTTGAAGAAGAAGCTTGAGGACTGTTGCAATGGCGATGTTGACGAAGCATATCCAAGTTCACTTCTTCCAACTTCCTTCCATTTCCTTCAATTTCCATTCCTGTAATCATCGTTCTCCCCCACGGATCTCCGCAGCTTTATCCTTTCGAAACCCTGATTCTCGACTCATTGAAGCTCGGAAACTCGTTGATGATTTCGACCCTCAAATTCCATTGGAGAAGGCCCTCACTCCACCCTCCTCCTGGTATATAGACCCTTCATTTTTTGCTCTCGAGCTCAATCATGTCTTCTACAGAGGATGGCAGGCTGTAGGTTCGCTTCCATCTCTAAATTTTTTGTGTTTTTTTTTTTACTCTATTTATGCCATGTATGTTTAGAATCACTTTGAAATATGTTTTTAATGATCGAAAATCAGTTTGGCTATATGAAACTTGTATTTAAAGTGTAGAATTAAATACTAAACTAATTTGGAGGGTTTAAAAATGTGTTTCCAAAATATTTTGAAGATGCCAAAAGTGACATTAGGAATTTTAAAATCACTCTGAAAATGCACTTAATTGTAGCTTCATCGAGAGGAGATTAATTGCTATATTTCAATGTGGCTGCAATTTGCGATCTATGATTATAATGGAAATTTCTTCCATTAACTGAGAACTCGGAAGTTCTTGGTCTTTGACAGAATACAAAGCAGGATAATCCCACTGAATTTGTGTACTCTTCTTTGTTTTACAGCTAATGCAATCTGCTCCTTGTTTTTCCCCTTTAGATTATAACTTTGACAATATTAAGGCATGATCATCAACTTTTTGTGCAATTGGCTTGTCAGGATATGTTGAACAGTTAAAAGATGCCCATGACTTTTTCACAGGCAGGTGTGATTAATTGTCACTTACCATTTGTTGCTGATAGTATAAACACTAGAGAGCCGTAATAAAATTATTTGTATTACCTGTATGTTGAATATATTTTTCACAGGGTAATTGAGCAAATATGGAGTTGAATCACTTGACATTTTAATGCCCTGAGTCTAAGTAATGGGATGCCAGAGATGTGTGTTCTTCCTCACCATGGCTTGTCCTTGAAATTTGTTTGCTCTGTCTCCTTGACTATGGCAGGTTGGGAAATGTAGAGTATGTGGTATGCAAAGATAATAACAGAAAGGTTCGTGCATTTCACAATGTTTGTCGCCATCATGCCTCACTTCTTGCGTCTGGATGTGGGAAGAAGTCGTGCTTTGTATGCCCATATCATGTGAGTTCACTTGTATATCTCCTTATCGGTTCCCTGGAAAGTTTTTAGTTTTCTATTTCCTAGTTATTTCTTGACGGCCAGGTTAAGGTTTTAGTCCATAACTTTACCATCATCGTGTAAGGAACAGTGATTTTCATTGAAGAAAAAAGAGATCATTTGTAAAGGTGAAAAATGGAAATCTCCAAGCTATGCTCTGATCTTTTGAAAAATATGTAAAATGTAACTAGGATGGAATATTATGCGATATTACGAGGTGCATCAATATGGGTTGTTACATATTTTGTGCAGGGATGGACATACGGGTTGGATGGAATACTGCTTAAGGCGACTAGAATAGATGGGATACAGAACTTTGATGAAAATGTATGTAATTATATCATTTTGATACCAGTTTCTACAATCATTTTAATGTAATATTTCTGAAAGGCACTTAGTTTATTTAATGCTGGAGATTAGGATTTTGGGCTTGTACCATTGCCAGTAGCTACGTGGGGGCCTTTCGTTCTTCTCAATTTGGATGGAAAATTATCATCTAAGCCAGATGTTGATGAAAATAAAGTGGCACGTGAATGGCTTGGAACATGTGCAGATGTGCTGAGGCTAAACGGAGTTGATGCTTCCCTAAGTTATGTCTGTCGACGTGAATACACTATTGAATGTAACTGGAAGGTATAGTCCTTGCATCCTAATTCTTAAAAAATGGTCATGTACTGTTACCACAATTCTTGTGGGTTTTGCTAATACGTTTCATTGGTTTTTTGCAGGTTTTTTGTGACAACTATTTAGATGGAGGATATCACGTTCCATATGCACATAAAGGGCTTGCATCTAATCTCAAGCTTGAGTCTTATTCTACAGAAGTATGTTGAATGTTTATTTACCGTGTAGTCAATCATTCACAGTAAATTATCAATGTTATGTGACTATAACTTGTGATGTAGCTATTTGAAACTGTTAGCATTCAAAGTTGTAAGGGTGGGGGAGAATCAAAAGGTGATGATTATGGTCGACTTGGATCAGAAGCACTCTATGCTTTTGTATACCCAAATTTCATGATAAATAGGTAGTTCCTGGCCATATCAATATACCTAGCTCTTTGGCTCTTTATGTTTTGAGATGTTAAGTCGTGATGGTCGTCTTATTATTTTTCGGCTTGTGTAATAGGTATGGACCTTGGATGGACACTAATCTAGTACTCCCACTTGGGCCTCGAAAATGTCTGGTGGTTTTTGATTATTTTCTTGAAGCTTCTTTTAAGGTAATTTCTGGTCCTTTGGCGTAATGGATATGTGATGTGCACAAGTTCTCACTTGTGTTCTTGATTTATTAAAAGGACTGCTCGTGTAACGCGAAAACTCAGCAAAAACCGAGCTTTTGTTGTATTGAAGTCTTTGGTTCATTTGGGTTCTTACCTTTTTTCTTCCTTTTATTTTTTATTTTTTTGAAATTAACTTTTTCGCTTCCTGATTTCATGTTCAGAATGATGACTCCTTTATACAACTAAGTTTAGAAGACAGTGAAAGCGTGCAGGTATTACGAACAAATCCACCCCTTTCCATTTTCATGTGTGCTGTGTCTGTTCTTTTTCTCTTACACATTGGTCCAAATAATATTCAGAATGAAGACATTATTCTGTGTGAAGGAGTTCAAAAGGGTCTCGAGTCACCAGCTTACAAGTTTGGCCGATATGCACCTTCGGTCGAGAATGCCATGCACCATTTCCATCGTCTTCTTCATTGTAACCTCACAAAATAAAAATAATTGAAAGAATGCCATGCACCATTTGCAAGATATTCTTTTAGGTTTTCATAGTTATCTTGGAAATGGTCTGAAATAACTTGCTTTTGTTACAGCACCTTTAGTTGTTGAAGCTGTGATATATACCAAATATGATAATTTTATTAATGTTTAAAAGTTGTGAATACAGTAAGGCAAGTAATTTCACAGCCCATTTGGATGATTTCTTTTATCTTCTCGAGTGACTAAATGTGATTCTTGATGAT

Coding sequence (CDS)

ATGGCGATGTTGACGAAGCATATCCAAGTTCACTTCTTCCAACTTCCTTCCATTTCCTTCAATTTCCATTCCTGTAATCATCGTTCTCCCCCACGGATCTCCGCAGCTTTATCCTTTCGAAACCCTGATTCTCGACTCATTGAAGCTCGGAAACTCGTTGATGATTTCGACCCTCAAATTCCATTGGAGAAGGCCCTCACTCCACCCTCCTCCTGGTATATAGACCCTTCATTTTTTGCTCTCGAGCTCAATCATGTCTTCTACAGAGGATGGCAGGCTGTAGGATATGTTGAACAGTTAAAAGATGCCCATGACTTTTTCACAGGCAGGTTGGGAAATGTAGAGTATGTGGTATGCAAAGATAATAACAGAAAGGTTCGTGCATTTCACAATGTTTGTCGCCATCATGCCTCACTTCTTGCGTCTGGATGTGGGAAGAAGTCGTGCTTTGTATGCCCATATCATGGATGGACATACGGGTTGGATGGAATACTGCTTAAGGCGACTAGAATAGATGGGATACAGAACTTTGATGAAAATGATTTTGGGCTTGTACCATTGCCAGTAGCTACGTGGGGGCCTTTCGTTCTTCTCAATTTGGATGGAAAATTATCATCTAAGCCAGATGTTGATGAAAATAAAGTGGCACGTGAATGGCTTGGAACATGTGCAGATGTGCTGAGGCTAAACGGAGTTGATGCTTCCCTAAGTTATGTCTGTCGACGTGAATACACTATTGAATGTAACTGGAAGGTTTTTTGTGACAACTATTTAGATGGAGGATATCACGTTCCATATGCACATAAAGGGCTTGCATCTAATCTCAAGCTTGAGTCTTATTCTACAGAACTATTTGAAACTGTTAGCATTCAAAGTTGTAAGGGTGGGGGAGAATCAAAAGGTGATGATTATGGTCGACTTGGATCAGAAGCACTCTATGCTTTTGTATACCCAAATTTCATGATAAATAGGTATGGACCTTGGATGGACACTAATCTAGTACTCCCACTTGGGCCTCGAAAATGTCTGGTGGTTTTTGATTATTTTCTTGAAGCTTCTTTTAAGAATGATGACTCCTTTATACAACTAAGTTTAGAAGACAGTGAAAGCGTGCAGAATGAAGACATTATTCTGTGTGAAGGAGTTCAAAAGGGTCTCGAGTCACCAGCTTACAAGTTTGGCCGATATGCACCTTCGGTCGAGAATGCCATGCACCATTTCCATCGTCTTCTTCATTGTAACCTCACAAAATAA

Protein sequence

MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNVCRHHASLLASGCGKKSCFVCPYHGWTYGLDGILLKATRIDGIQNFDENDFGLVPLPVATWGPFVLLNLDGKLSSKPDVDENKVAREWLGTCADVLRLNGVDASLSYVCRREYTIECNWKVFCDNYLDGGYHVPYAHKGLASNLKLESYSTELFETVSIQSCKGGGESKGDDYGRLGSEALYAFVYPNFMINRYGPWMDTNLVLPLGPRKCLVVFDYFLEASFKNDDSFIQLSLEDSESVQNEDIILCEGVQKGLESPAYKFGRYAPSVENAMHHFHRLLHCNLTK
Homology
BLAST of Cucsat.G7371 vs. ExPASy Swiss-Prot
Match: Q9SZR0 (Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g29890 PE=2 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 1.4e-19
Identity = 49/92 (53.26%), Postives = 62/92 (67.39%), Query Frame = 0

Query: 20  FNFHSCNHRSPPRISAALSFRNPDS--RLIEARKLVDDFDPQIPLEKALTPPSSWYIDPS 79
           FN HS    S  + S    F NP     + +  KLV +FDP+IPLE+A TPPSSWY DP 
Sbjct: 24  FNSHSEFGVSISKFSRR-RFHNPTRVFAVSDISKLVTEFDPKIPLERASTPPSSWYTDPQ 83

Query: 80  FFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 110
           F++ EL+ VFY GWQAVGY +Q+K++ DFFTG
Sbjct: 84  FYSFELDRVFYGGWQAVGYSDQIKESRDFFTG 114

BLAST of Cucsat.G7371 vs. ExPASy Swiss-Prot
Match: Q9LKN0 (Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.8e-17
Identity = 35/60 (58.33%), Postives = 48/60 (80.00%), Query Frame = 0

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           + LV DFDP +P E ALTPPSSWY +P+F+A EL+ +FY+GWQ  GY +Q+K+A+ +FTG
Sbjct: 80  QSLVKDFDPLVPAEDALTPPSSWYTEPAFYAHELDRIFYKGWQVAGYSDQVKEANQYFTG 139

BLAST of Cucsat.G7371 vs. ExPASy Swiss-Prot
Match: O22553 (Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-16
Identity = 34/60 (56.67%), Postives = 47/60 (78.33%), Query Frame = 0

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           R LV +FDP+IP E ALTPPS+WY +P+F++ EL  +FY+GWQ  GY EQ+K+ + +FTG
Sbjct: 86  RSLVHEFDPEIPPEDALTPPSTWYTEPAFYSHELERIFYKGWQVAGYSEQVKEKNQYFTG 145

BLAST of Cucsat.G7371 vs. ExPASy Swiss-Prot
Match: Q93XE1 (Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 7.9e-15
Identity = 30/79 (37.97%), Postives = 53/79 (67.09%), Query Frame = 0

Query: 31  PRISAALSFRNPDSRLIEARKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRG 90
           P I ++++  N  +     ++++ +FDP++P E   TPPS+WY DPS ++ EL+ +F +G
Sbjct: 63  PVIPSSINSNNITTTTPNIKRIIHEFDPKVPAEDGFTPPSTWYTDPSLYSHELDRIFSKG 122

Query: 91  WQAVGYVEQLKDAHDFFTG 110
           WQ  GY +Q+K+ + +FTG
Sbjct: 123 WQVAGYSDQIKEPNQYFTG 141

BLAST of Cucsat.G7371 vs. ExPASy Swiss-Prot
Match: O04121 (Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-14
Identity = 32/60 (53.33%), Postives = 45/60 (75.00%), Query Frame = 0

Query: 50  RKLVDDFDPQIPLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           + LV +FDPQIP E A TPPSSWY +P+F++ EL  +FY+GWQ  G  +Q+K+ + +FTG
Sbjct: 79  QSLVHEFDPQIPPEDAHTPPSSWYTEPAFYSHELERIFYKGWQVAGISDQIKEPNQYFTG 138

BLAST of Cucsat.G7371 vs. NCBI nr
Match: XP_004139149.1 (choline monooxygenase, chloroplastic isoform X1 [Cucumis sativus] >KGN66608.1 hypothetical protein Csa_007257 [Cucumis sativus])

HSP 1 Score: 226 bits (577), Expect = 1.28e-70
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MAMLTKHIQVHFFQLPS SFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI
Sbjct: 1   MAMLTKHIQVHFFQLPSTSFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. NCBI nr
Match: XP_008443631.1 (PREDICTED: choline monooxygenase, chloroplastic isoform X2 [Cucumis melo] >KAA0060178.1 choline monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 207 bits (526), Expect = 5.83e-63
Identity = 98/109 (89.91%), Postives = 103/109 (94.50%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. NCBI nr
Match: TYK04233.1 (choline monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 207 bits (526), Expect = 5.83e-63
Identity = 98/109 (89.91%), Postives = 103/109 (94.50%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. NCBI nr
Match: XP_038880722.1 (choline monooxygenase, chloroplastic [Benincasa hispida])

HSP 1 Score: 194 bits (494), Expect = 3.61e-58
Identity = 92/109 (84.40%), Postives = 98/109 (89.91%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHI  HFFQ PSISFN HSCNHRSP RISAALSFRN DS  IEA+KLVD+FDP+I
Sbjct: 1   MATLTKHIHTHFFQPPSISFNLHSCNHRSPSRISAALSFRNSDSHFIEAQKLVDEFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKA+TPPSSWYIDPSF+ALEL+ VFYRGWQAVGYVEQLKD HDFFTG
Sbjct: 61  PLEKAVTPPSSWYIDPSFYALELDRVFYRGWQAVGYVEQLKDPHDFFTG 109

BLAST of Cucsat.G7371 vs. NCBI nr
Match: XP_016899776.1 (PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 195 bits (495), Expect = 4.01e-58
Identity = 97/128 (75.78%), Postives = 103/128 (80.47%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAV-------------------GYVEQLK 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQA+                   GYVEQLK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAIITLTILRHGSSTFCAIGLSGYVEQLK 120

BLAST of Cucsat.G7371 vs. ExPASy TrEMBL
Match: A0A0A0LXX3 (Choline monooxygenase, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_1G641020 PE=3 SV=1)

HSP 1 Score: 226 bits (577), Expect = 6.18e-71
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MAMLTKHIQVHFFQLPS SFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI
Sbjct: 1   MAMLTKHIQVHFFQLPSTSFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. ExPASy TrEMBL
Match: A0A5A7UY48 (Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold542G00540 PE=3 SV=1)

HSP 1 Score: 207 bits (526), Expect = 2.82e-63
Identity = 98/109 (89.91%), Postives = 103/109 (94.50%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. ExPASy TrEMBL
Match: A0A5D3BWX2 (Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold758G00070 PE=3 SV=1)

HSP 1 Score: 207 bits (526), Expect = 2.82e-63
Identity = 98/109 (89.91%), Postives = 103/109 (94.50%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. ExPASy TrEMBL
Match: A0A1S3B9A0 (Choline monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487183 PE=3 SV=1)

HSP 1 Score: 207 bits (526), Expect = 2.82e-63
Identity = 98/109 (89.91%), Postives = 103/109 (94.50%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAVGYVEQLKDAHDFFTG 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQAVGYVEQLKDAHDFFTG
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAVGYVEQLKDAHDFFTG 109

BLAST of Cucsat.G7371 vs. ExPASy TrEMBL
Match: A0A1S4DUW5 (choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487183 PE=4 SV=1)

HSP 1 Score: 195 bits (495), Expect = 1.94e-58
Identity = 97/128 (75.78%), Postives = 103/128 (80.47%), Query Frame = 0

Query: 1   MAMLTKHIQVHFFQLPSISFNFHSCNHRSPPRISAALSFRNPDSRLIEARKLVDDFDPQI 60
           MA LTKHIQ+HFFQ PSISFNFH CNHRSP RISA+LSFR+PDSR IEARKLVDDFDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 61  PLEKALTPPSSWYIDPSFFALELNHVFYRGWQAV-------------------GYVEQLK 109
           PLEKALTPPSSWYIDPSFFALEL+ VFYRGWQA+                   GYVEQLK
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQAIITLTILRHGSSTFCAIGLSGYVEQLK 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZR01.4e-1953.26Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g2989... [more]
Q9LKN03.8e-1758.33Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 ... [more]
O225531.9e-1656.67Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1[more]
Q93XE17.9e-1537.97Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2... [more]
O041211.0e-1453.33Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_004139149.11.28e-7099.08choline monooxygenase, chloroplastic isoform X1 [Cucumis sativus] >KGN66608.1 hy... [more]
XP_008443631.15.83e-6389.91PREDICTED: choline monooxygenase, chloroplastic isoform X2 [Cucumis melo] >KAA00... [more]
TYK04233.15.83e-6389.91choline monooxygenase [Cucumis melo var. makuwa][more]
XP_038880722.13.61e-5884.40choline monooxygenase, chloroplastic [Benincasa hispida][more]
XP_016899776.14.01e-5875.78PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0LXX36.18e-7199.08Choline monooxygenase, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_1G641020 ... [more]
A0A5A7UY482.82e-6389.91Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3BWX22.82e-6389.91Choline monooxygenase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B9A02.82e-6389.91Choline monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487183 PE=... [more]
A0A1S4DUW51.94e-5875.78choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015879Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domainPFAMPF00848Ring_hydroxyl_Acoord: 15..97
e-value: 1.8E-12
score: 47.6
NoneNo IPR availableGENE3D3.90.380.10coord: 2..105
e-value: 1.7E-20
score: 75.6
NoneNo IPR availablePANTHERPTHR43756:SF5CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 9..105
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 11..100
IPR044637Aromatic-ring-hydroxylating dioxygenasePANTHERPTHR43756CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 9..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G7371.T1Cucsat.G7371.T1mRNA
Cucsat.G7371.T11Cucsat.G7371.T11mRNA
Cucsat.G7371.T2Cucsat.G7371.T2mRNA
Cucsat.G7371.T8Cucsat.G7371.T8mRNA
Cucsat.G7371.T12Cucsat.G7371.T12mRNA
Cucsat.G7371.T3Cucsat.G7371.T3mRNA
Cucsat.G7371.T13Cucsat.G7371.T13mRNA
Cucsat.G7371.T5Cucsat.G7371.T5mRNA
Cucsat.G7371.T4Cucsat.G7371.T4mRNA
Cucsat.G7371.T9Cucsat.G7371.T9mRNA
Cucsat.G7371.T15Cucsat.G7371.T15mRNA
Cucsat.G7371.T16Cucsat.G7371.T16mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0019285 glycine betaine biosynthetic process from choline
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
cellular_component GO:0031967 organelle envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0019133 choline monooxygenase activity
molecular_function GO:0005506 iron ion binding