Cucsat.G5932.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G5932.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Regulator of Vps4 activity in the MVB pathway protein
Location	ctg1402: 2617136 .. 2618916 (-)
RNA-Seq Expression	Cucsat.G5932.T2
Synteny	Cucsat.G5932.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGTTTCTTCTTCACACTGCCTCTCCCTCTCCGAAACATCTTTCATATATAAACAAAACCTCATTCAAAGTTCCATCCAATAACCCCTTTTAACCCATTTCTATTTCCATTCCCTTTCGCCATGGGCAGAAAGCTTGATGCTCTTCTTGGTAGGAATTTCAGAGCCTCCAAATTTCGCCCTCTTCTCAATCTCTCCCTCTCTCGTCTTTCTATTCTCACCGCCCAACGCCGTGTCGGATGCTCTCAGGCCAATTCCGATGTCCTTCAACTCCTTCAACTCTCCCACCACCACCGTGCTTTACTTCGAGTACGTTTCTCTTCTCTTCTTTTCATTTCCCATTTCAATTTCATTTTATTCATTTCTTTTCTGTTTTTTCATTCATAGGTCGAGAAAGTCATCAAGGATCAGAATGCTCTGGATGCTTATGTTTTAATTGAAGGCTACCTTAATCTCTTGCTTGAAAGAACCACTCTCCTCGAACAACAAAGGTTTGTTTTCTTATTCATCTCTCCTCTACCCTCCTTCTTAATCATTTGATTTGATTTTACTTATTATTATTTGTTTTTATTGCAGTGAGTGCCCTGAAGAATTGAAAGAGGCAGTTGCTGGGTTACTCTTTGCAGCTTCAAGATGTGGGGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCACTCGTTTTGGCAAGGAATTCACTGCACGTGCTGTTGAATTACGCAACAATTGTGGAGTTAATCTTTCGGTAAATTTTGGGTTCTCTTCATTAACTCCTGCTTCATTCCTTTCTTTTATTCATTCTTTGCTCTCTCTCTGTAGTTAATGCAAAAACTTTCCACAAGGCAGCCCACTTTGGAAACTAGAATGGACGCCCTCAAATCCATTGCTTCCGAGAATGGGATTGTATTGCAAATTGACCAACTCCCTTCTTCCAAACAGGTATACAACTTGAACTTTGACATTTTATTTTACTGCTTACAGCTGTGTTTTGGTAGCAGAGTCGAAACCCGAAATCTTCAATCTTTGTAAAACCCAATTTGGGATTCTTTTTCTGATTATCGCAGGAAAAAGTAGGCAGAAACGGGAGGCAAAGCGAGGCAGAGGGACAGAGTGAAGAATTTTCAAATGAAGTGGCATCTGGTTCAAAAACAACGTACAAAGATGTGGCGGATGCAGCACAAGCCGCTTTTGAATCAGCAGCTCAAGCAGCAGCAGCTGCACGAGCTGCCATGGAGCTCTCCCGACCCCATGAGGGTCCAAGCAGCCCGAGTAAGCCTGGGTCTGGAACAACTTCAGATAACAAACAAAAGAAAGAGAAAAGAGAGGTTGAATCTAAAGTAAAACAAGAAATGGAGGAATATGGAAATGGGAGGAAAGGAGAAGGAGAAAGGGAAGAAGAAGAAGGGGATGGAATGGATGAGGAGAGAACTTTAAATGGGTTGAAGATGGAAACAAAGGTAGAGAAAACAGAGGTAAGTGAGAAGGGAAGCTTTCGTTTAAATCTGGAGAAGAAACCAATTTCAGTAAGAACAAGAAGAGTAGGAGGATACTGAAGAAGAACAATATATAAGTGGTTCGTAATTGGGTTTGCTTTTGAATTCTTTCATCATATGTGTAATACATTTTTTGGTTTGTGGGAATATAATGGAAATTGTGTAGTTGTGTGGGTTGGGAATTCTGGTATCGTATGGTAAATACAACATTTTTGTTTGTTAGACAATGGTATATTCACATTAATATTTGACGACCACTTTTTTTGTTTTGAACTCCAAATTATTCA

Coding sequence (CDS)

ATGGGCAGAAAGCTTGATGCTCTTCTTGGTAGGAATTTCAGAGCCTCCAAATTTCGCCCTCTTCTCAATCTCTCCCTCTCTCGTCTTTCTATTCTCACCGCCCAACGCCGTGTCGGATGCTCTCAGGCCAATTCCGATGTCCTTCAACTCCTTCAACTCTCCCACCACCACCGTGCTTTACTTCGAGTCGAGAAAGTCATCAAGGATCAGAATGCTCTGGATGCTTATGTTTTAATTGAAGGCTACCTTAATCTCTTGCTTGAAAGAACCACTCTCCTCGAACAACAAAGTGAGTGCCCTGAAGAATTGAAAGAGGCAGTTGCTGGGTTACTCTTTGCAGCTTCAAGATGTGGGGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCACTCGTTTTGGCAAGGAATTCACTGCACGTGCTGTTGAATTACGCAACAATTGTGGAGTTAATCTTTCGGTAAATTTTGGGTTCTCTTCATTAACTCCTGCTTCATTCCTTTCTTTTATTCATTCTTTGCTCTCTCTCTGTAGTTAA

Protein sequence

MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTPASFLSFIHSLLSLCS

Homology

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.5e-11
Identity = 43/147 (29.25%), Postives = 79/147 (53.74%), Query Frame = 0


Query: 10  GRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVIKD 69
           G ++ + K +  L L++SR+ IL  ++         +V +LL+  +   A +RVE +I+D
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSV 129
           +  ++ + +IE    LL  R  L+   +E P E+KE++  L++++ R    PEL +IK+ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 LTTRFGKEFTARAVELRNNCGVNLSVN 157
           L  ++GK      +E   NC  +  VN
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVN 145

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.3e-10
Identity = 39/132 (29.55%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEF 139
              L  ++ KE+
Sbjct: 121 ADQLCAKYSKEY 132

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.3e-10
Identity = 39/132 (29.55%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEF 139
              L  ++ KE+
Sbjct: 121 ADQLCAKYSKEY 132

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.3e-10
Identity = 39/132 (29.55%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEF 139
              L  ++ KE+
Sbjct: 121 ADQLCAKYSKEY 132

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.6e-09
Identity = 38/132 (28.79%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG   +A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEF 139
              L  ++ KE+
Sbjct: 121 ADQLCAKYSKEY 132

[Close]

BLAST of Cucsat.G5932.T2 vs. NCBI nr
Match: XP_011656519.1 (uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical protein Csa_005294 [Cucumis sativus])

HSP 1 Score: 300 bits (767), Expect = 6.17e-99
Identity = 156/164 (95.12%), Postives = 158/164 (96.34%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLS+    S+  P
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. NCBI nr
Match: XP_008445650.1 (PREDICTED: uncharacterized protein LOC103488606 [Cucumis melo])

HSP 1 Score: 294 bits (753), Expect = 3.68e-97
Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR+ CSQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLS+    S+  P
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. NCBI nr
Match: XP_038884116.1 (uncharacterized protein LOC120075040 [Benincasa hispida])

HSP 1 Score: 286 bits (733), Expect = 1.41e-93
Identity = 148/152 (97.37%), Postives = 150/152 (98.68%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNL+LSRLSILTAQRRV CSQANSDVLQLLQL +HHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLSILTAQRRVRCSQANSDVLQLLQLPNHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVN 152
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVN 152

[Close]

BLAST of Cucsat.G5932.T2 vs. NCBI nr
Match: XP_022139373.1 (uncharacterized protein LOC111010325 [Momordica charantia])

HSP 1 Score: 261 bits (668), Expect = 1.56e-83
Identity = 135/164 (82.32%), Postives = 147/164 (89.63%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNL+LSRL++LT QR V  SQA SD LQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIK+QNALDAYVLIEGYLNLL+ERT LLEQ+ ECPEELKEAV+GL+FAASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PEL EIKSVLTTRFGKEFTARAVELRNNCGV+ S+    S+  P
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. NCBI nr
Match: XP_023538782.1 (uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260 bits (665), Expect = 3.94e-83
Identity = 134/152 (88.16%), Postives = 143/152 (94.08%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL+LSRL+ILT QRR+  SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLRHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ERT+LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVN 152
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVN 152

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 300 bits (767), Expect = 2.99e-99
Identity = 156/164 (95.12%), Postives = 158/164 (96.34%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLS+    S+  P
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL
Match: A0A1S3BD83 (uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=3 SV=1)

HSP 1 Score: 294 bits (753), Expect = 1.78e-97
Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR+ CSQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLS+    S+  P
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL
Match: A0A6J1CFG6 (uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010325 PE=3 SV=1)

HSP 1 Score: 261 bits (668), Expect = 7.57e-84
Identity = 135/164 (82.32%), Postives = 147/164 (89.63%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNL+LSRL++LT QR V  SQA SD LQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIK+QNALDAYVLIEGYLNLL+ERT LLEQ+ ECPEELKEAV+GL+FAASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 164
           PEL EIKSVLTTRFGKEFTARAVELRNNCGV+ S+    S+  P
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 260 bits (665), Expect = 1.91e-83
Identity = 134/152 (88.16%), Postives = 143/152 (94.08%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL+LSRL+ILT QRR+  SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVN 152
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVN 152

[Close]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 256 bits (655), Expect = 6.19e-82
Identity = 131/152 (86.18%), Postives = 142/152 (93.42%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL++SRL+ILT QRR+  +QA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVL+EGYLNLL+ERT+LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVN 152
           PELHEIKSVLTT FGKEFTARAVELRNNCGVN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVN 152

[Close]

BLAST of Cucsat.G5932.T2 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 177.9 bits (450), Expect = 7.0e-45
Identity = 91/164 (55.49%), Postives = 119/164 (72.56%), Query Frame = 0


Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60
           MG+KLDALLGR+F+ +KF+ L+ L+L+RLSIL  QR+   SQA SDV +LL+L  H  A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
            RV++V+KDQN LD    I GY  L L+R  L E   +CPEEL EAV+GLLFAASR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTP 165
           PEL EI++VL +RFGK+  AR++ELR+NCGV+  +    S+  P
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPP 164

[Close]

BLAST of Cucsat.G5932.T2 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 111.7 bits (278), Expect = 6.2e-25
Identity = 53/146 (36.30%), Postives = 90/146 (61.64%), Query Frame = 0


Query: 7   ALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVEKV 66
           +L  R F +SK +    ++++R+ ++  +R V   Q   D+  LLQ      A +RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEI 126
           I++QN   A  +IE +  L++ R T++ +Q +CP +LKE +A L+FAA RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 KSVLTTRFGKEFTARAVELRNNCGVN 153
           + +   ++GK+F + A +LR +CGVN
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVN 165

[Close]

BLAST of Cucsat.G5932.T2 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-24
Identity = 57/161 (35.40%), Postives = 94/161 (58.39%), Query Frame = 0


Query: 5   LDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVE 64
           L+ L  R    +K +  LNL+++R+ +L  +R +       ++   LQ      A +RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  KVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            VI++ N   AY ++E +   +L R  +LE + ECP EL+EA+A ++FAA RC + P+L 
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVNLSVNFGFSSLTPA 166
           +IK++  T++GKEF   A ELR + GVN ++    S  +P+
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPS 164

[Close]

BLAST of Cucsat.G5932.T2 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-23
Identity = 50/148 (33.78%), Postives = 90/148 (60.81%), Query Frame = 0


Query: 5   LDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVE 64
           LD+   + F+A+K + LL L++ R+ ++  +R     Q   ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  KVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            +I+++  + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVN 153
           +++ +  +++GKEF A A EL+ + GVN
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVN 151

[Close]

BLAST of Cucsat.G5932.T2 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-23
Identity = 50/148 (33.78%), Postives = 90/148 (60.81%), Query Frame = 0


Query: 5   LDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRALLRVE 64
           LD+   + F+A+K + LL L++ R+ ++  +R     Q   ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  KVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            +I+++  + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVN 153
           +++ +  +++GKEF A A EL+ + GVN
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVN 151

The following BLAST results are available for this feature:

BLAST of Cucsat.G5932.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54I39	6.5e-11	29.25	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]
P53990	9.3e-10	29.55	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
Q9CX00	9.3e-10	29.55	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
Q568Z6	9.3e-10	29.55	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]
Q3ZBV1	4.6e-09	28.79	IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1	[more]

BLAST of Cucsat.G5932.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011656519.1	6.17e-99	95.12	uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical ...	[more]
XP_008445650.1	3.68e-97	93.29	PREDICTED: uncharacterized protein LOC103488606 [Cucumis melo]	[more]
XP_038884116.1	1.41e-93	97.37	uncharacterized protein LOC120075040 [Benincasa hispida]	[more]
XP_022139373.1	1.56e-83	82.32	uncharacterized protein LOC111010325 [Momordica charantia]	[more]
XP_023538782.1	3.94e-83	88.16	uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G5932.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAU0	2.99e-99	95.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1	[more]
A0A1S3BD83	1.78e-97	93.29	uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=...	[more]
A0A6J1CFG6	7.57e-84	82.32	uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1E0Z0	1.91e-83	88.16	uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC1114298...	[more]
A0A6J1I2V3	6.19e-82	86.18	uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447...	[more]

BLAST of Cucsat.G5932.T2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13340.1	7.0e-45	55.49	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G35730.1	6.2e-25	36.30	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G25420.1	1.1e-24	35.40	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.1	1.5e-23	33.78	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.2	1.5e-23	33.78	Regulator of Vps4 activity in the MVB pathway protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 21..157 e-value: 1.2E-40 score: 139.1
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 8..153
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 6..169 e-value: 2.2E-49 score: 169.5
None	No IPR available	PANTHER	PTHR12161:SF16	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 8..153

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G5932	Cucsat.G5932	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5932.T2.E1	Cucsat.G5932.T2.E1	exon
Cucsat.G5932.T2.E2	Cucsat.G5932.T2.E2	exon
Cucsat.G5932.T2.E3	Cucsat.G5932.T2.E3	exon
Cucsat.G5932.T2.E4	Cucsat.G5932.T2.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5932.T2.C3	Cucsat.G5932.T2.C3	CDS
Cucsat.G5932.T2.C2	Cucsat.G5932.T2.C2	CDS
Cucsat.G5932.T2.C1	Cucsat.G5932.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G5932.T2	Cucsat.G5932.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport