Cucsat.G4359.T20 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G4359.T20 vs. ExPASy Swiss-Prot
Match: P42534 (Putative polyketide hydroxylase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO5321 PE=3 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 2.8e-49
Identity = 198/680 (29.12%), Postives = 290/680 (42.65%), Query Frame = 0

Query: 49  GGDET-MVPVLIVGAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFR 108
           GGD T  VPVL+VG   VGL  ++ L +LGV+  +VE++   S HP+    N RTME+FR
Sbjct: 14  GGDRTHRVPVLVVGGSLVGLSTSVFLGRLGVRHTLVERHAGTSIHPRGRGNNVRTMEIFR 73

Query: 109 KLDGLAEKIQLYQPPVESWRKFIYCTSLNGT----ILGSVDHMQPQDFEHIISPVSVAHF 168
            + G    I+     +      +   +L G     +   +D   P       SP S    
Sbjct: 74  -VAGTEPDIRRAAATLADNHGILQAPTLAGDAGEWLFKQID---PGGGLARFSPSSWCLC 133

Query: 169 SQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYL 228
           SQ  L   LL    NLG                   +  G E +S +A  E V       
Sbjct: 134 SQNDLEPELLTHATNLG-----------------GDLRFGTELLSFEADTEGVTAIVKSR 193

Query: 229 KEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKD 288
           + G+H     I  + LV ADG  S VR  +GI   G  DL   VSI F SR L + ++ D
Sbjct: 194 ETGEHT---TIRADYLVAADGPRSPVREQLGIGQSGPGDLFHNVSITFRSRRLAD-VVGD 253

Query: 289 RPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQ-QNIEDFFPQMCEELIFKLVGRN 348
           R  ++ ++ +  A G L+  D ++  ++   P++P Q + +EDF  + C   I + +G  
Sbjct: 254 RRFIVCYLTDENADGALLPVDNRE-NWVFHAPWHPEQGETVEDFTDERCAAHIRRAIGDP 313

Query: 349 LCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWK 408
             D+++    PW     VA  +  R   VLLAGD+AH   P G FG NTGIQD HNLAWK
Sbjct: 314 DLDVEITGKAPWHAAQRVARSY--RSGRVLLAGDSAHEMSPTGAFGSNTGIQDAHNLAWK 373

Query: 409 LAAVLQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVV 468
           LAAVL+  A  ++L+TY+ ERRP+A    A S +    ++E        P +A       
Sbjct: 374 LAAVLEGWAGEALLDTYDTERRPVA---EATSARAAHRSVEHSHPGFAPPPVAGG----G 433

Query: 469 NHGLGSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKS---- 528
             G G+   +   +    G    G L           P G             G +    
Sbjct: 434 GPGAGTPGGAGRGTGGPGGPGGPGGLGGPGGPGGTGGPGGPGGPGGPDGPRGAGGAPGGG 493

Query: 529 ---------LQLQFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLP-SADPGSRLP 588
                     Q       LG+RY  GA++       G +  T    + L  +  PGSR P
Sbjct: 494 PGGGPGGGGPQRGILNVALGYRYPRGAVV-------GADPATPVVPEGLDLTGAPGSRAP 553

Query: 589 HMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILW 648
           H+ VR    ++ +STLDL      + L++++  ++      AA  VA   +  +K     
Sbjct: 554 HLWVR--RGQDRLSTLDLYE----DSLVLLSDAAQPTGWHEAAAGVAAGMRVPLK----- 613

Query: 649 SANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWRV 708
              + ++  S    L P +                W     +T  GA+LVRPD  +AWR 
Sbjct: 614 ---SYRVGGSPGADLNPDDE------------ETDWARAHGVTRGGAVLVRPDGFVAWR- 624

BLAST of Cucsat.G4359.T20 vs. ExPASy Swiss-Prot
Match: Q05355 (Putative polyketide hydroxylase OS=Streptomyces halstedii OX=1944 GN=schC PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 1.4e-45
Identity = 135/396 (34.09%), Postives = 190/396 (47.98%), Query Frame = 0

Query: 43  NSKIFHGGDET-MVPVLIVGAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNR 102
           N++    GD    VPVL+VG   VGL  ++ L +LGV+  +VE++   S HP+    N R
Sbjct: 2   NARADRAGDTVHRVPVLVVGGSLVGLSTSVFLGRLGVRHMLVERHAGTSVHPRGRGNNVR 61

Query: 103 TMEVFRKLDGLAEKIQLYQPPVESWRKFIYCTSLNGT----ILGSVDHMQPQDFEHIISP 162
           TMEV+R   G+ + I+     +      +   SL G     +L  +D   P       SP
Sbjct: 62  TMEVYRAA-GVEQGIRRAAATLAGNHGILQTPSLVGDEGEWLLRDID---PGGGLARFSP 121

Query: 163 VSVAHFSQYKLNGLLLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVN 222
            S    SQ  L  +LL     LG                  +I    E  S +     V 
Sbjct: 122 SSWCLCSQNDLEPVLLDHAVELG-----------------GEIRFSTELQSFEQDPAGVT 181

Query: 223 MTASYLKEGKHVERRNISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELG 282
                 + G+H     +  + LV ADG  S VR  +GI   G  DL   VS+ F SR L 
Sbjct: 182 AVIKSRRSGEHT---TVRADYLVAADGPRSPVREQLGIGQSGPGDLFHNVSVTFRSRRLA 241

Query: 283 EYLLKDRPGMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYP-PQQNIEDFFPQMCEELIF 342
           E+ + DR  ++ ++ N EA G L+  D ++  ++   P+YP   + +EDF  + C + I 
Sbjct: 242 EF-VGDRHFIVCYLTNPEADGALLPVDNRE-NWVFHAPWYPRAARPLEDFTDERCADHIR 301

Query: 343 KLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDV 402
           + VG    D+++    PW     VA ++  R   V LAGD+AH   P G FG NTGIQD 
Sbjct: 302 RAVGVPDLDVEITGKAPWHAAQRVARQY--RAGRVFLAGDSAHEMSPTGAFGSNTGIQDA 361

Query: 403 HNLAWKLAAVLQDIASPSILNTYEMERRPIALFNTA 433
           HNLAWKLAAVL   A   +L+TY+ ERRP+A   TA
Sbjct: 362 HNLAWKLAAVLGGWAGDGLLDTYDAERRPVAEATTA 369

BLAST of Cucsat.G4359.T20 vs. ExPASy Swiss-Prot
Match: Q8KN28 (2,4-dichlorophenol 6-monooxygenase OS=Delftia acidovorans OX=80866 GN=tfdB PE=1 SV=3)

HSP 1 Score: 181.8 bits (460), Expect = 2.7e-44
Identity = 176/643 (27.37%), Postives = 260/643 (40.44%), Query Frame = 0

Query: 57  VLIVGAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKI 116
           VL+VG+GP G    +LL   GVK   V K  + S+ P++H  N RTMEV R L GL  + 
Sbjct: 12  VLVVGSGPAGAASTLLLATYGVKTLCVSKYATTSRTPRSHITNQRTMEVMRDL-GLELEC 71

Query: 117 QLYQPPVESWRKFIYCTSLNGTILGSV----DHMQPQDFEHIISPVSVAHFSQYKLNGLL 176
           +    P E   + +YCTSL G  LG V     H Q +    + SP  +    Q  L  ++
Sbjct: 72  EAMASPAELMGENVYCTSLVGDELGRVLTWGTHPQRRADYELASPTHMCDLPQNLLEPIM 131

Query: 177 LKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERR 236
           +      G  V                     E VS+   +  V  T   +++     + 
Sbjct: 132 INHAARRGADV-----------------RFHTEFVSLKQDETGVTAT---VRDHLLDRQY 191

Query: 237 NISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIF 296
           +I    L+GADGA S V   VG+ M+G+  +   +++  F  +L +Y +  RP +LY++ 
Sbjct: 192 DIRAKYLIGADGANSQVVDQVGLPMEGKMGVSGSINV-VFEADLTKY-VGHRPSVLYWVI 251

Query: 297 ----NTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDV 356
               +   +G+ V   ++     L +  Y       D       +++  L+G +   + +
Sbjct: 252 QPGSSVGGLGIGVIRMVRPWNKWLCIWGYDIAGGPPDLNEAHARQIVHSLLGDSTIPVKI 311

Query: 357 RDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 416
                W ++   A +     N V   GDA HR PP  G G NT IQD  NL WKL+ VLQ
Sbjct: 312 ESTSTWTVNDMYATRLF--DNRVFCMGDAVHRHPPTNGLGSNTSIQDAFNLCWKLSHVLQ 371

Query: 417 DIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVPAALGL-DPKIANSVHRVVNHGLG 476
             A P +L TY  ER P+A      + K+      + AALGL D K    + R       
Sbjct: 372 GKAGPELLATYNEERAPVARQVVQRANKSLGDFPPILAALGLFDTKDPEQMQR------- 431

Query: 477 SILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAED 536
                            I RL+        ++P    + A LR   D G          +
Sbjct: 432 ----------------NIARLK-------EQSPEAQEQRAALRAAID-GTQYVYNAHGVE 491

Query: 537 LGFRYSEGAIIRDNNLLGGREEPTGRRRQYL---PSADPGSRLPHMNVRVLASEEIISTL 596
           +  RY   AI+ D     G  +P  RR   L    S  PG+ +PH  V V      +STL
Sbjct: 492 MNQRYQSAAIVPD-----GTPDPGFRRDSELYHAHSGRPGAPVPH--VWVTRHGRRVSTL 551

Query: 597 DLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLT 656
           DL    +   L  IA          AA+  AE     + V I+            ++   
Sbjct: 552 DLCGKGRFSLLSGIA----GSPWVEAAVHAAESLGIDLDVHII---------GPGQELED 563

Query: 657 PWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWR 688
            + ++  V+EI +S               GA+LVRPD  I WR
Sbjct: 612 LYGDFARVREIEES---------------GALLVRPDNFICWR 563

BLAST of Cucsat.G4359.T20 vs. ExPASy Swiss-Prot
Match: P27138 (2,4-dichlorophenol 6-monooxygenase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=tfdB PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 1.0e-43
Identity = 175/662 (26.44%), Postives = 278/662 (41.99%), Query Frame = 0

Query: 57  VLIVGAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKI 116
           VL+VG GP G     LL + GV+  ++ K    +  P+AH  N RTME+ R L GL  + 
Sbjct: 9   VLVVGTGPAGASAGALLARYGVRTMLINKYNWTAPTPRAHITNQRTMEILRDL-GLEAEA 68

Query: 117 QLYQPPVESWRKFIYCTSLNGTILGSV-----DHMQPQDFEHIISPVSVAHFSQYKLNGL 176
           +LY  P +   +   C SL G   G +     D  +  D++   SP S+    Q  L  +
Sbjct: 69  RLYAAPNDLMGENTICASLAGEEFGRIRTWGTDVRRRADYDE-CSPTSMCDLPQNYLEPI 128

Query: 177 LLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVER 236
           L+K             +L+G C VR     LGHE        +  +  +S L++  + E 
Sbjct: 129 LVKSA-----------ALDG-CKVRFDTEYLGHE--------QDADGVSSRLRDRLNGEE 188

Query: 237 RNISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIH-FFSRELGEYLLKDRPGMLYF 296
             +    L+GADGA S V  +  +++  E  + K  SI+  F  +L  Y +  RP +LY+
Sbjct: 189 FTVRSKYLIGADGANSRV--VSDLDLPLEGTMGKSGSINLLFEADLDRY-VAHRPSVLYW 248

Query: 297 IF----NTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDI 356
           +     +   +G+ V   ++     L +  Y  +Q   +        ++  L+G +   +
Sbjct: 249 VIQPGSDIGGLGIGVVRMVRPWNKWLAIWGYDVEQGPPEISESFARRIVHNLIGDDSVPL 308

Query: 357 DVRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAV 416
            +  +  W ++   A +   +Q  V  AGDA HR PP  G G NT IQD  NLAWK+A V
Sbjct: 309 KIEGISTWTVNDMYATRL--QQGRVFCAGDAVHRHPPTNGLGSNTSIQDSFNLAWKIAMV 368

Query: 417 LQDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGL 476
           L   A  S+L+TY +ER PIA      + K+ +    +  ALGL P+  ++         
Sbjct: 369 LNGTADESLLDTYTIERAPIAKQVVCRANKSLEDFPPIAMALGL-PQAKSA--------- 428

Query: 477 GSILSSSLQSAVLDGIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAE 536
              + S++      G                  P   ++  +LR     G +        
Sbjct: 429 -DEMKSNMARRKEPG------------------PEAQAQRTRLREAI-AGTNYVYNAHGV 488

Query: 537 DLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDL 596
           ++  RY   AI+ DN+     E        +  S  PG+ +PH+ V        IST DL
Sbjct: 489 EMNQRYDSPAIVADNS---PDEVFRDVELYHQASTRPGAPMPHVWVYASGDGHRISTKDL 548

Query: 597 VSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPW 656
               K  F L       +++ A AA  V+ +   +V V I+      +            
Sbjct: 549 CG--KGNFTLFTGIGGAAWQDAAAA--VSRQLGVAVTVRIIGPGQAYE------------ 581

Query: 657 ENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTT 709
           ++Y +   I + I            D GAILVRPD H+A+R  S +  D   +L+     
Sbjct: 609 DHYGDFARISEII------------DTGAILVRPDFHVAYRATS-LPADAAGDLVSAMRR 581

BLAST of Cucsat.G4359.T20 vs. ExPASy Swiss-Prot
Match: P31020 (Phenol 2-monooxygenase OS=Pseudomonas sp. (strain EST1001) OX=69012 GN=pheA PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.8e-35
Identity = 173/664 (26.05%), Postives = 259/664 (39.01%), Query Frame = 0

Query: 57  VLIVGAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKI 116
           VLIVG+GP G   A+ L+  G+   ++ K +  +  P+AH  N RTME+ R   G+ +++
Sbjct: 38  VLIVGSGPAGSSAAMFLSTQGISNIMITKYRWTANTPRAHITNQRTMEILRDA-GIEDQV 97

Query: 117 QLYQPPVESWRKFIYCTSLNGTILG-----SVDHMQPQDFEHIISPVSVAHFSQYKLNGL 176
                P E     +YC S+ G  +G          +  D+E + SP       Q  L  +
Sbjct: 98  LAEAVPHELMGDTVYCESMAGEEIGRRPTWGTRPDRRADYE-LASPAMPCDIPQTLLEPI 157

Query: 177 LLKQLQNLGFQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVER 236
           +LK                    +R  +     E +S    D+ V++       G   + 
Sbjct: 158 MLKN-----------------ATMRGTQTQFSTEYLSHTQDDKGVSVQVLNRLTG---QE 217

Query: 237 RNISCNILVGADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFI 296
             I    L+GADGA S V   +G  M   N   K    H+    L   L    P  + + 
Sbjct: 218 YTIRAKYLIGADGARSKVAADIGGSM---NITFKADLSHWRPSALDPVL--GLPPRIEYR 277

Query: 297 FNTEAIGVLVAHDLKQGEFILQVPF----YPPQQNIEDFFPQMCEELIFKLVGRNLCDID 356
           +       +V       E+++   F     PP+ N ++       +++  LVG    D++
Sbjct: 278 WPRRWFDRMVR---PWNEWLVVWGFDINQEPPKLNDDEAI-----QIVRNLVGIEDLDVE 337

Query: 357 VRDVKPWIMHAEVAEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVL 416
           +     W  + + A     ++  V  AGDA H+ PP+ G G NT IQD +NL WKLA VL
Sbjct: 338 ILGYSLWGNNDQYATHL--QKGRVCCAGDAIHKHPPSHGLGSNTSIQDSYNLCWKLACVL 397

Query: 417 QDIASPSILNTYEMERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLG 476
           +  A P +L TY  ER PIA                                ++V    G
Sbjct: 398 KGQAGPELLETYSTERAPIA-------------------------------KQIVTRANG 457

Query: 477 SILSSSLQSAVLDGIFKIGRLQLSDTF-----LNVEN-PIGSSRLAKLRHIFDEGKSLQL 536
              SSS    + D +  +     +D F     L  EN P G+ R A LR   D  K  + 
Sbjct: 458 ---SSSEYKPIFDAL-GVTDATTNDEFVEKLALRKENSPEGARRRAALRAALD-NKDYEF 517

Query: 537 QFPAEDLGFRYSEGAIIRDNNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEII 596
                ++G  Y   A+I D        E      Q   S  PG RLPH    +  ++E  
Sbjct: 518 NAQGTEIGQFYDSSAVITDGQKRPAMTEDPMLHHQ--KSTFPGLRLPH--AWLGDAKEKY 577

Query: 597 STLDLVSGDKIEFLLIIAPRSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKD 656
           ST D+  G +      I  ++     A AA++VAE     +K  ++            +D
Sbjct: 578 STHDIAEGTRFTIFTGITGQA----WADAAVRVAERLGIDLKAVVIGEGQPV------QD 595

Query: 657 QLTPWENYIEVQEIRQSITSPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELI 706
               W    EV E                   G ILVRPD+HI WR +S ++ DP T L 
Sbjct: 638 LYGDWLRQREVDE------------------DGVILVRPDKHIGWRAQSMVA-DPETALF 595

BLAST of Cucsat.G4359.T20 vs. NCBI nr
Match: XP_004142293.1 (uncharacterized protein LOC101210766 [Cucumis sativus] >XP_031742115.1 uncharacterized protein LOC101210766 [Cucumis sativus])

HSP 1 Score: 1425 bits (3688), Expect = 0.0
Identity = 709/709 (100.00%), Postives = 709/709 (100.00%), Query Frame = 0

Query: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60
           MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV
Sbjct: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60

Query: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120
           GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ
Sbjct: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120

Query: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180
           PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG
Sbjct: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180

Query: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240
           FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV
Sbjct: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240

Query: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300
           GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL
Sbjct: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300

Query: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360
           VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV
Sbjct: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360

Query: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420
           AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420

Query: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480
           MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD
Sbjct: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480

Query: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540
           GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD
Sbjct: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540

Query: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600
           NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP
Sbjct: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600

Query: 601 RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT 660
           RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT
Sbjct: 601 RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT 660

Query: 661 SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK
Sbjct: 661 SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709

BLAST of Cucsat.G4359.T20 vs. NCBI nr
Match: KAE8648228.1 (hypothetical protein Csa_018353 [Cucumis sativus])

HSP 1 Score: 1414 bits (3659), Expect = 0.0
Identity = 703/705 (99.72%), Postives = 704/705 (99.86%), Query Frame = 0

Query: 5    LNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGP 64
            + RAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGP
Sbjct: 310  VQRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGP 369

Query: 65   VGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVE 124
            VGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVE
Sbjct: 370  VGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVE 429

Query: 125  SWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVC 184
            SWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVC
Sbjct: 430  SWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVC 489

Query: 185  SPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADG 244
            SPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADG
Sbjct: 490  SPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADG 549

Query: 245  AGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHD 304
            AGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHD
Sbjct: 550  AGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHD 609

Query: 305  LKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKF 364
            LKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKF
Sbjct: 610  LKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKF 669

Query: 365  ICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERR 424
            ICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERR
Sbjct: 670  ICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERR 729

Query: 425  PIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFK 484
            PIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFK
Sbjct: 730  PIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFK 789

Query: 485  IGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLL 544
            IGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLL
Sbjct: 790  IGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLL 849

Query: 545  GGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSES 604
            GGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSES
Sbjct: 850  GGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSES 909

Query: 605  YRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSW 664
            YRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSW
Sbjct: 910  YRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSW 969

Query: 665  WDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
            WDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK
Sbjct: 970  WDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 1014

BLAST of Cucsat.G4359.T20 vs. NCBI nr
Match: XP_008454726.1 (PREDICTED: putative polyketide hydroxylase [Cucumis melo] >XP_008454728.1 PREDICTED: putative polyketide hydroxylase [Cucumis melo])

HSP 1 Score: 1347 bits (3485), Expect = 0.0
Identity = 667/709 (94.08%), Postives = 688/709 (97.04%), Query Frame = 0

Query: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60
           M+LFLNRAMGFLGFF+RFNGLQKFDA  R  PL  +QCRGSSNSK+FHGGDETMVPVLIV
Sbjct: 1   MSLFLNRAMGFLGFFRRFNGLQKFDATPRAIPLGYVQCRGSSNSKLFHGGDETMVPVLIV 60

Query: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120
           GAGPVGLVLAILLTKLG+KCAIVEKN+SFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ
Sbjct: 61  GAGPVGLVLAILLTKLGIKCAIVEKNRSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120

Query: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180
           PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLG
Sbjct: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLG 180

Query: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240
           FQV SPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV
Sbjct: 181 FQVGSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240

Query: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300
           GADGAGSTVRRLVG+EMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL
Sbjct: 241 GADGAGSTVRRLVGVEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300

Query: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360
           VAHDLKQGEFILQVPFYPPQQNIEDF P MC ELIFKLVG+NLCDIDV+DVKPWIMHAEV
Sbjct: 301 VAHDLKQGEFILQVPFYPPQQNIEDFCPAMCNELIFKLVGQNLCDIDVQDVKPWIMHAEV 360

Query: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420
           AEKFIC +NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 361 AEKFICCRNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420

Query: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480
           MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVN+GLGSILSSSLQSAVLD
Sbjct: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNNGLGSILSSSLQSAVLD 480

Query: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540
           GIFKIGRLQLSD FLNV+NPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYS+GAII D
Sbjct: 481 GIFKIGRLQLSDIFLNVKNPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSDGAIIPD 540

Query: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600
           N LLGGREEPTGRRRQY+PSADPGSRLPHMNVRVLASE+IISTLDLVSGDKIEFLLIIAP
Sbjct: 541 NTLLGGREEPTGRRRQYIPSADPGSRLPHMNVRVLASEDIISTLDLVSGDKIEFLLIIAP 600

Query: 601 RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT 660
           RSESY LAHA  KVAEEFKTSVKVCILWSA+TTKIESSSKD LTPWENY++V+EIRQS T
Sbjct: 601 RSESYHLAHAGFKVAEEFKTSVKVCILWSASTTKIESSSKDLLTPWENYVDVEEIRQSTT 660

Query: 661 SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           SPSWWD+CKMTDKGAILVRPDEHIAWRVKSGISGDPNTEL+ VFTTLLK
Sbjct: 661 SPSWWDICKMTDKGAILVRPDEHIAWRVKSGISGDPNTELMRVFTTLLK 709

BLAST of Cucsat.G4359.T20 vs. NCBI nr
Match: XP_038892396.1 (2,4-dichlorophenol 6-monooxygenase isoform X1 [Benincasa hispida] >XP_038892397.1 2,4-dichlorophenol 6-monooxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 1278 bits (3306), Expect = 0.0
Identity = 629/701 (89.73%), Postives = 668/701 (95.29%), Query Frame = 0

Query: 9   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 68
           MGFLGFF+RFNGLQKFDA LR  P+  +Q R  SNSK+FHGG ETMVPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFRRFNGLQKFDATLRAIPVGYVQSRCLSNSKLFHGGKETMVPVLIVGAGPVGLV 60

Query: 69  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 128
           LAILLTKLGVKCA+VEKN+SFSKHPQAHFINNR+MEVFRKLDGLAE+IQL+QPPVESWRK
Sbjct: 61  LAILLTKLGVKCAVVEKNRSFSKHPQAHFINNRSMEVFRKLDGLAEEIQLHQPPVESWRK 120

Query: 129 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS 188
           FIYCTSLNGTILGSVDHMQPQDFEHI SPVSVAHFSQYKLN LLLKQLQNLGFQVC+P+S
Sbjct: 121 FIYCTSLNGTILGSVDHMQPQDFEHITSPVSVAHFSQYKLNRLLLKQLQNLGFQVCTPES 180

Query: 189 LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST 248
           LEGPCVVREKKILLGHECVSIDATDE++ MTASYLKEG H++RRNISCN+LVGADGAGS+
Sbjct: 181 LEGPCVVREKKILLGHECVSIDATDETITMTASYLKEGTHIKRRNISCNVLVGADGAGSS 240

Query: 249 VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG 308
           VRRLVGIEMKGENDLQKLVS+HFFSRELGEYLLK+RPGMLYF+FNTEAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGENDLQKLVSVHFFSRELGEYLLKERPGMLYFVFNTEAIGVLVAHDLKQG 300

Query: 309 EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ 368
           EFILQVPFYPPQQNI DF P+MCEELIFKLVG+NLCDIDV+DVKPWIMHAEVAEKFIC Q
Sbjct: 301 EFILQVPFYPPQQNIGDFCPKMCEELIFKLVGQNLCDIDVQDVKPWIMHAEVAEKFICCQ 360

Query: 369 NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 428
           + VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL
Sbjct: 361 SRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 420

Query: 429 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL 488
           FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSS QSAVLDGIFKIGRL
Sbjct: 421 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSQQSAVLDGIFKIGRL 480

Query: 489 QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE 548
           QLSD+FLN  NP+GSSRLAKLR IFDEGKSLQLQFPAEDLGFRYSEGAII D++LLGGRE
Sbjct: 481 QLSDSFLNDGNPVGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGAIIPDDSLLGGRE 540

Query: 549 EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA 608
           EPTGRRRQY+PSADPGSRLPHMNVRVLASEEI STLDLVSGD ++FLLII PR ESY LA
Sbjct: 541 EPTGRRRQYVPSADPGSRLPHMNVRVLASEEIFSTLDLVSGDVVQFLLIIGPRPESYCLA 600

Query: 609 HAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVC 668
           HA LKVAEEFK SV+VCILWSA+TTKI+SSSK++LTPW+NYI+VQEIRQ  TSPSWWDVC
Sbjct: 601 HATLKVAEEFKISVRVCILWSADTTKIQSSSKEELTPWKNYIDVQEIRQWSTSPSWWDVC 660

Query: 669 KMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           +MTDKGAILVRPDEHIAWRVKSGISGDPNTEL  VFTTLLK
Sbjct: 661 QMTDKGAILVRPDEHIAWRVKSGISGDPNTELTRVFTTLLK 701

BLAST of Cucsat.G4359.T20 vs. NCBI nr
Match: KAG7012746.1 (hypothetical protein SDJN02_25499, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1234 bits (3192), Expect = 0.0
Identity = 608/703 (86.49%), Postives = 651/703 (92.60%), Query Frame = 0

Query: 8   AMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGL 67
           AMGFLGFFKRFNGLQKF+A LR  PL  +Q RG SNSK+FHGG+ET VPVLIVGAGPVGL
Sbjct: 33  AMGFLGFFKRFNGLQKFNASLRAIPLGYVQSRGLSNSKLFHGGEETAVPVLIVGAGPVGL 92

Query: 68  VLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWR 127
           VLAILLTKLGVKCAI+EKN SFS HPQAHFINNR+MEVFRKLDGLAE+IQL QPPV+SWR
Sbjct: 93  VLAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLRQPPVDSWR 152

Query: 128 KFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPD 187
           KFIYCTSL GTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLGFQVCSPD
Sbjct: 153 KFIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPD 212

Query: 188 SLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGS 247
           S EGPC+VREK+IL+GHECVSI  TD++V MTASYLKEGKH+ERRNI  NILVGADGAGS
Sbjct: 213 SSEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGS 272

Query: 248 TVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQ 307
           TVRRLVGIEMKGE DLQKLVSIHFFSRELGEYLL +RPGMLYFIFNTEAIGVLVAHDLKQ
Sbjct: 273 TVRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLYFIFNTEAIGVLVAHDLKQ 332

Query: 308 GEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICR 367
           GEFILQVPFYPPQQNIEDF P+MCEE+IF LVG NLCD+DV+DVKPWIMHAEVAEKFI  
Sbjct: 333 GEFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQDVKPWIMHAEVAEKFIFC 392

Query: 368 QNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIA 427
           +  VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIA
Sbjct: 393 EGRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIA 452

Query: 428 LFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGR 487
           L NTALSVKNFKAAME+PAALGLDPKIANSVH+ VNHG+GS+LSSSLQ  VLDGIFKIGR
Sbjct: 453 LLNTALSVKNFKAAMEIPAALGLDPKIANSVHQAVNHGIGSVLSSSLQRTVLDGIFKIGR 512

Query: 488 LQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGR 547
           LQLSDT LN +NP+GSSRLAKL HIFDEGKSLQLQFPAEDLGFRYSEGA++ DNN  GGR
Sbjct: 513 LQLSDTLLNDKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALLPDNNQPGGR 572

Query: 548 EEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRL 607
           EEPTGRRR+Y+PSADPGS+LPHMNVR LAS E+ISTLDLVSGDK+EFLLIIAP  ESYRL
Sbjct: 573 EEPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPESYRL 632

Query: 608 AHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSP-SWWD 667
           A AAL VAEEFKTSV+VCILWSA+ T+IESSSK++LTPWENYI+VQEIRQ  TS  SWWD
Sbjct: 633 ARAALMVAEEFKTSVRVCILWSADITRIESSSKEELTPWENYIDVQEIRQPSTSTASWWD 692

Query: 668 VCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           VC+MTDKGAILVRPDEH+ WRVKSG+SGDPNTEL  VFTTLLK
Sbjct: 693 VCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTLLK 735

BLAST of Cucsat.G4359.T20 vs. ExPASy TrEMBL
Match: A0A0A0KLD9 (FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G056620 PE=4 SV=1)

HSP 1 Score: 1409 bits (3648), Expect = 0.0
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 9   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 68
           MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 60

Query: 69  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 128
           LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK
Sbjct: 61  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 120

Query: 129 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS 188
           FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS
Sbjct: 121 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS 180

Query: 189 LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST 248
           LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST
Sbjct: 181 LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST 240

Query: 249 VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG 308
           VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG 300

Query: 309 EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ 368
           EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ
Sbjct: 301 EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ 360

Query: 369 NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 428
           NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL
Sbjct: 361 NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 420

Query: 429 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL 488
           FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL
Sbjct: 421 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL 480

Query: 489 QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE 548
           QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE
Sbjct: 481 QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE 540

Query: 549 EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA 608
           EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA
Sbjct: 541 EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA 600

Query: 609 HAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVC 668
           HAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVC
Sbjct: 601 HAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDVC 660

Query: 669 KMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           KMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK
Sbjct: 661 KMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 701

BLAST of Cucsat.G4359.T20 vs. ExPASy TrEMBL
Match: A0A1S3BZD5 (putative polyketide hydroxylase OS=Cucumis melo OX=3656 GN=LOC103495067 PE=4 SV=1)

HSP 1 Score: 1347 bits (3485), Expect = 0.0
Identity = 667/709 (94.08%), Postives = 688/709 (97.04%), Query Frame = 0

Query: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60
           M+LFLNRAMGFLGFF+RFNGLQKFDA  R  PL  +QCRGSSNSK+FHGGDETMVPVLIV
Sbjct: 1   MSLFLNRAMGFLGFFRRFNGLQKFDATPRAIPLGYVQCRGSSNSKLFHGGDETMVPVLIV 60

Query: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120
           GAGPVGLVLAILLTKLG+KCAIVEKN+SFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ
Sbjct: 61  GAGPVGLVLAILLTKLGIKCAIVEKNRSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120

Query: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180
           PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLG
Sbjct: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLG 180

Query: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240
           FQV SPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV
Sbjct: 181 FQVGSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240

Query: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300
           GADGAGSTVRRLVG+EMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL
Sbjct: 241 GADGAGSTVRRLVGVEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300

Query: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360
           VAHDLKQGEFILQVPFYPPQQNIEDF P MC ELIFKLVG+NLCDIDV+DVKPWIMHAEV
Sbjct: 301 VAHDLKQGEFILQVPFYPPQQNIEDFCPAMCNELIFKLVGQNLCDIDVQDVKPWIMHAEV 360

Query: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420
           AEKFIC +NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 361 AEKFICCRNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420

Query: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480
           MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVN+GLGSILSSSLQSAVLD
Sbjct: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNNGLGSILSSSLQSAVLD 480

Query: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540
           GIFKIGRLQLSD FLNV+NPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYS+GAII D
Sbjct: 481 GIFKIGRLQLSDIFLNVKNPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSDGAIIPD 540

Query: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600
           N LLGGREEPTGRRRQY+PSADPGSRLPHMNVRVLASE+IISTLDLVSGDKIEFLLIIAP
Sbjct: 541 NTLLGGREEPTGRRRQYIPSADPGSRLPHMNVRVLASEDIISTLDLVSGDKIEFLLIIAP 600

Query: 601 RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT 660
           RSESY LAHA  KVAEEFKTSVKVCILWSA+TTKIESSSKD LTPWENY++V+EIRQS T
Sbjct: 601 RSESYHLAHAGFKVAEEFKTSVKVCILWSASTTKIESSSKDLLTPWENYVDVEEIRQSTT 660

Query: 661 SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           SPSWWD+CKMTDKGAILVRPDEHIAWRVKSGISGDPNTEL+ VFTTLLK
Sbjct: 661 SPSWWDICKMTDKGAILVRPDEHIAWRVKSGISGDPNTELMRVFTTLLK 709

BLAST of Cucsat.G4359.T20 vs. ExPASy TrEMBL
Match: A0A6J1G1R0 (uncharacterized protein LOC111449881 OS=Cucurbita moschata OX=3662 GN=LOC111449881 PE=4 SV=1)

HSP 1 Score: 1233 bits (3190), Expect = 0.0
Identity = 611/710 (86.06%), Postives = 653/710 (91.97%), Query Frame = 0

Query: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60
           ++LF    MGFLGFFKRFNGLQKF+A LR  PL  +Q RG SNSK+FHGG+ET VPVLIV
Sbjct: 26  LSLFSVTDMGFLGFFKRFNGLQKFNASLRAIPLGYVQSRGLSNSKLFHGGEETAVPVLIV 85

Query: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120
           GAGPVGLVLAILLTKLGVKCAI+EKN SFS HPQAHFINNR+MEVFRKLDGLAE+IQL Q
Sbjct: 86  GAGPVGLVLAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLRQ 145

Query: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180
           PPV+SWRKFIYCTSL GTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNL 
Sbjct: 146 PPVDSWRKFIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLE 205

Query: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240
           FQVCSPDS EGPC+VREK+IL+GHECVSI  TD++V MTASYLKEGKH+ERRNI  NILV
Sbjct: 206 FQVCSPDSSEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILV 265

Query: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300
           GADGAGSTVRRLVGIEMKGE DLQKLVSIHFFSRELGEYLL +RPGMLYFIFNTEAIGVL
Sbjct: 266 GADGAGSTVRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLYFIFNTEAIGVL 325

Query: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360
           VAHDLKQGEFILQVPFYPPQQNIEDF P+MCEE+IF LVG NLCD+DV+DVKPWIMHAEV
Sbjct: 326 VAHDLKQGEFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQDVKPWIMHAEV 385

Query: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420
           AEKFI  Q  VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 386 AEKFISCQGRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 445

Query: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480
           MER+PIAL NTALSVKNFKAAMEVPAALGLDPKIANSVH+ VNHGLGS+LSSSLQ  VLD
Sbjct: 446 MERKPIALLNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSVLSSSLQREVLD 505

Query: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540
           GIFKIGRLQLSDT LN +NP+GSSRLAKL HIFDEGKSLQLQFPAEDLGFRYSEGA++ D
Sbjct: 506 GIFKIGRLQLSDTLLNDKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALLPD 565

Query: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600
           NN  GGREEPTGRRR+Y+PSADPGS+LPHMNVR LAS E+ISTLDLVSGDK+EFLLIIAP
Sbjct: 566 NNQPGGREEPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAP 625

Query: 601 RSESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSIT 660
             ESYRLA AAL VAEEFKTSV+VCILWSA+ T+IESSSK++LTPWENYI+VQEIRQ  T
Sbjct: 626 LPESYRLARAALMVAEEFKTSVRVCILWSADITRIESSSKEELTPWENYIDVQEIRQPST 685

Query: 661 SP-SWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           S  SWWDVC+MTDKGAILVRPDEH+ WRVKSG+SGDPNTEL  VFTTLLK
Sbjct: 686 STASWWDVCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTLLK 735

BLAST of Cucsat.G4359.T20 vs. ExPASy TrEMBL
Match: A0A6J1HUM9 (uncharacterized protein LOC111466353 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466353 PE=4 SV=1)

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 606/702 (86.32%), Postives = 650/702 (92.59%), Query Frame = 0

Query: 9   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 68
           MGFLGFFKRFNGL++F+A LR  PL  +Q RG SNSK+FHGG+ET VPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLRRFNASLRAIPLGYVQSRGLSNSKLFHGGEETAVPVLIVGAGPVGLV 60

Query: 69  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 128
           LAILLTKLGVKCAI+EKN SFS HPQAHFINNR+MEVFRKLDGLAE+IQL QPPV+SWRK
Sbjct: 61  LAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLCQPPVDSWRK 120

Query: 129 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS 188
           F+YCTSL GTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN LLLKQLQNLGFQVCSPDS
Sbjct: 121 FLYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPDS 180

Query: 189 LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST 248
            EGPC+VREK+IL+GHECVSI  TD++V MTASYLKEGKH+ERRNI  NILVGADGAGST
Sbjct: 181 SEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGST 240

Query: 249 VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG 308
           VRRLVGIEMKGE +LQKLVSIHFFSRELGEYLL +RPGMLYFIFN EAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGEKNLQKLVSIHFFSRELGEYLLTERPGMLYFIFNIEAIGVLVAHDLKQG 300

Query: 309 EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ 368
           EFILQVPFYPPQQNIEDF P+MCEE+IF LVG NLCD+DV+DVKPWIMHAEVAEKFI  Q
Sbjct: 301 EFILQVPFYPPQQNIEDFCPKMCEEIIFNLVGLNLCDVDVQDVKPWIMHAEVAEKFIACQ 360

Query: 369 NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 428
           N VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIAL
Sbjct: 361 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIAL 420

Query: 429 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL 488
            NTALSVKNFKAAMEVPAALGLDPKIANSVH+ VNHGLGS+LSSSLQ  VLDGIFKIGRL
Sbjct: 421 LNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSVLSSSLQRTVLDGIFKIGRL 480

Query: 489 QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE 548
           QLSDT LN +NP+GSSRLAKL HIFDEGKSLQLQFPAEDLGFRYSEGA+I DNN LGGRE
Sbjct: 481 QLSDTLLNEKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALIPDNNQLGGRE 540

Query: 549 EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA 608
           EPTGRRR+Y+PSADPGS+LPHMNVR LAS E+ISTLDLVSGDK+EFLLIIAP  E YRLA
Sbjct: 541 EPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPEFYRLA 600

Query: 609 HAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSP-SWWDV 668
            AAL VAEEFKTSV+VCILWSA+ T+IESSSK++L PWENYI+VQEIRQ  TS  SWWDV
Sbjct: 601 RAALMVAEEFKTSVRVCILWSADITRIESSSKEELAPWENYIDVQEIRQPSTSTASWWDV 660

Query: 669 CKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           C+MTDKGAILVRPDEH+AWRVKSG+SGDPNTEL  VFT+LLK
Sbjct: 661 CQMTDKGAILVRPDEHVAWRVKSGVSGDPNTELRRVFTSLLK 702

BLAST of Cucsat.G4359.T20 vs. ExPASy TrEMBL
Match: A0A6J1DCK1 (uncharacterized protein LOC111019789 OS=Momordica charantia OX=3673 GN=LOC111019789 PE=4 SV=1)

HSP 1 Score: 1219 bits (3153), Expect = 0.0
Identity = 602/710 (84.79%), Postives = 651/710 (91.69%), Query Frame = 0

Query: 1   MNLFLNRAMGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIV 60
           ++LF  R MG LGF  RFNGL+KFDA LR  PL  +Q RG SNSKIFHGG++TMVPVLIV
Sbjct: 13  LSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIV 72

Query: 61  GAGPVGLVLAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120
           GAGPVGLVLAILLTKLGVKCA+VEKN+SFS HPQAHFINNR+MEVFRKL GLAE+IQL Q
Sbjct: 73  GAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQ 132

Query: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLG 180
           PPV+SWRKFIYCTSLNG ILGSVDHMQPQDF +IISPVSVAHFSQYKLN LLLK+LQNLG
Sbjct: 133 PPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLG 192

Query: 181 FQVCSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240
           FQVCSPDSLE  C+VREK+ILLGHECVSIDATD+SV  TASYLKEGKH ERRNI  NILV
Sbjct: 193 FQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILV 252

Query: 241 GADGAGSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300
           G DGAGSTVRRLVGIE+KGENDLQKLVS+HFFSRELGEYLL +RPGMLYFIFNTEAIGVL
Sbjct: 253 GTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVL 312

Query: 301 VAHDLKQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEV 360
           VAHDLKQGEFILQVPFYPPQQ+IEDF P+MCEELIFKLVG NLCDIDV+DVKPWIMHAEV
Sbjct: 313 VAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEV 372

Query: 361 AEKFICRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420
           AEKFIC  N VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 373 AEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 432

Query: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLD 480
           MERRPIA FNTALSVKNFKAAMEVPAALGLDP+IANSVH+ VNHGLGS+L SSLQS+VLD
Sbjct: 433 MERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLD 492

Query: 481 GIFKIGRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRD 540
           GIFKIGR+QLS++ LN +NPIGSSRLAKLR IFDEGKSLQLQFPAEDLGFRYSEGA+I D
Sbjct: 493 GIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPD 552

Query: 541 NNLLGGREEPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAP 600
           N LL G EEPTGRRRQY+PSADPGSRLPHMNVR LASE++ISTLDLVSGDK+EFLLIIAP
Sbjct: 553 NTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAP 612

Query: 601 RSESYRLAHAALKVA-EEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSI 660
             ESY LA +ALK+A EEFKTSVKVC+LWSA+  ++ES S+ +LTPWENY++VQEIRQS 
Sbjct: 613 LPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSS 672

Query: 661 TSPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 709
           TSPSWWDVC+MTD GAILVRPDEHIAWRVKSG+SGD NT++  VFT LLK
Sbjct: 673 TSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722

BLAST of Cucsat.G4359.T20 vs. TAIR 10
Match: AT1G24340.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 931.0 bits (2405), Expect = 5.6e-271
Identity = 449/708 (63.42%), Postives = 562/708 (79.38%), Query Frame = 0

Query: 9   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 68
           M  LG  KR   +   ++ +R  P+R  Q +  S++ +F+G D   +PVLIVGAGPVGLV
Sbjct: 1   MAILGLIKRVTRITVNNSRVRVYPVRYFQRKDLSSTNLFNGEDAAKLPVLIVGAGPVGLV 60

Query: 69  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 128
           L+ILLTKLGVKCA+V+K  SFSKHPQAHFINNR+ME+FR+LDGLAE+I+  QPPV+ WRK
Sbjct: 61  LSILLTKLGVKCAVVDKATSFSKHPQAHFINNRSMEIFRELDGLAEEIERSQPPVDLWRK 120

Query: 129 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQV---CS 188
           FIYCTSL+G+ LG+VDHMQPQDFE ++SP SVAHFSQYKL  LLLK+L++LGF V     
Sbjct: 121 FIYCTSLSGSTLGTVDHMQPQDFEKVVSPASVAHFSQYKLTNLLLKRLEDLGFHVRGSKE 180

Query: 189 PDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGA 248
            D LE   VV  ++IL+GHECV IDA  +S+  T S+LK GKH+ +RNI C++LVGADGA
Sbjct: 181 SDGLEADSVV-ARQILMGHECVGIDANKDSITATVSFLKGGKHM-KRNIQCSLLVGADGA 240

Query: 249 GSTVRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDL 308
           GS VR+L  IEM+GE DLQKLVS+HF SRELGEYL+ +RPGML+FIFNT+ IGVLVAHDL
Sbjct: 241 GSAVRKLTVIEMRGERDLQKLVSVHFMSRELGEYLISNRPGMLFFIFNTDGIGVLVAHDL 300

Query: 309 KQGEFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFI 368
            QGEF+LQ+P+YPPQQ++ DF P+MC+ LIF LVG  L D+DV D+KPW+MHAEVAEKF+
Sbjct: 301 LQGEFVLQIPYYPPQQSLSDFSPEMCKMLIFNLVGHELSDLDVADIKPWVMHAEVAEKFM 360

Query: 369 CRQNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRP 428
           C +N V+LAGDAAHRFPPAGGFGMNTGIQD HNLAWK+AA++Q  A+ SIL TYE ERRP
Sbjct: 361 CCENRVILAGDAAHRFPPAGGFGMNTGIQDAHNLAWKIAALVQGSANSSILKTYETERRP 420

Query: 429 IALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKI 488
           IAL NT+LSV+NF+AAM VP+ALGLDP +ANSVHR +N  +GSIL + LQ A+LD +F +
Sbjct: 421 IALSNTSLSVQNFRAAMSVPSALGLDPTVANSVHRFINKTVGSILPTGLQKAILDNVFAL 480

Query: 489 GRLQLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDN-NLL 548
           GR QLS++ LN  NP+G+ RL++L+ IF+ GKSLQLQFPAEDLGFRY EGAI+ DN +  
Sbjct: 481 GRAQLSESLLNESNPLGNQRLSRLKSIFEGGKSLQLQFPAEDLGFRYLEGAIVPDNESEA 540

Query: 549 GGREEPTGRRRQYLPSADPGSRLPHMNVRVLAS---EEIISTLDLVSGDKIEFLLIIAPR 608
           G  E P+GRRR Y+P A+PGSRLPHM V++L+    E I+STLDLVS +K+EFLLII+P 
Sbjct: 541 GDPEVPSGRRRDYVPCAEPGSRLPHMYVKILSDSTREVIVSTLDLVSTEKVEFLLIISPL 600

Query: 609 SESYRLAHAALKVAEEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEI-RQSIT 668
            ESY LAHA  KVA+EF  SVKVC++W ++   +E  S   L PWENY++V E+ +Q+  
Sbjct: 601 QESYELAHATFKVAKEFMASVKVCVVWPSSDDGLERKSNSALAPWENYVDVMEVKKQNGE 660

Query: 669 SPSWWDVCKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLL 709
             SWW +CKM+++G+ILVRPD+HIAWR KSGI+ DP   +  VFT +L
Sbjct: 661 GTSWWSICKMSERGSILVRPDQHIAWRAKSGITLDPTLHMRDVFTIIL 706