Cucsat.G4075.T2 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G4075.T2
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAB hydrolase-1 domain-containing protein
Locationctg105: 3643730 .. 3646506 (-)
RNA-Seq ExpressionCucsat.G4075.T2
SyntenyCucsat.G4075.T2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAATAGGAAAAGAAGCCATGCAGTAGAAATTTTTTTGTTCAATTATTTCATCATACATGACACCTGTCCAAACGTGTCTCTCAAAGTTATTTAATGCTAGTAAAACCATTTGTTTTCTTCACAATTCCTCCGATTTTTTCTTCCTTGAATACACAAAAGCTTAAGTTCAAAGCAACTCATCAATGGTCAACATCTTCCACATCTACGACCAGGTTCTTCGTTGGGTAATGAAAGCAGCCGGAATCAAACCGGTTCAGCTTCAAATCAAACCCGGAACCGCCATGAACTTCTGGGTTCCCGCCGGAAAACCAAACAAAAACCACCAAAACCATCCTCCCCTCCTTTTCCTCCATGGGTTCGCCACCAATGCCATTATGACATGGCAATTCCAAGTCCTGAAGTTCGCCAAAAACCACGCCGTTTATGTACCGGACTTCATGTTCTTCGGGGATTCCGTTACGGATCGGCCGGACCGATCGACGGAGTTTCAGGCGGAGTGCGTGGTGGAGGGGTTGAGAAAATTGGGTGTGGACCGAAGGTTTGTTTTGGTGGGGTTTAGTTATGGGGCGATGGTGGGGTTTAGATTGGCGGAGATGTATCCGGAAATGGTGGAGGCGATGGTGGTTACGGCGGCGCCGACGGTGCTGACGGAAAGGATTACCGGAGAGGCAATGGAAAAAATAGGGTATAAGAGTTGGTCTGAATATTTGATACCGGAGACGGTGAAGGGTGCTATGTCTATGCTTGAAATTGCTTCCTTTGAATTTCCACGATTTCCACGTTGGATTTTCAAGCAATATCTGGAGGTACGTCAAAATTATTTTTGTAAAACCATTTCTTTTTTATTTATTTATTTTCCTTACGGAGAATTCAATGGTAAATAACCATAGATTGAAAAAAAAACATTTTTTTTGTGGGATATGGTTTTTAAAAGTTATTCTACTCATTTAACTATTTTATTTAAAATAAAAGTATAAAGTTATTTTTAAAAAACATTTTGAAACTCAAAATTCCCATTTTTAAAACGTATAGATCAAACCTATATATTCATCAAAACTTACAAACTAAAAAGATTAATATTTTTAAATATTTATTAACACTAGACAAGGTTGAATGTCATTAATATCGCAAATATTTAGGTCCTTTTGAAAACAAGTTTTGTGAAAAACTCAAGCTCTTAGTTAAATTCTAAAATCCCAAAACTTGCATTTAAAAAACCAAAACATGCAAACAAATAATTAACAAGTGAGTGCAATCAGATCAAAAATTGGATTCATTATTATTTTCTAGAATATAAAGAGTCATTTACGATAATTATTTGTAATTTTTTAAAAATGTTATTATATATTTAATTATTTTAAAATTAAATTATAAGGTAGAGTAGAGACCCATAAAGTTGCCATGCGTGCAGTTATTAGAGACGACCTCAGCAGATTTATAATATACATTTTTGTACATTATTATTGATGATGGGAAAACTGATTTGAAATTAGTATTTTGTTTTGTTAATAAAAGGAAAAGGATGGTTGTGATTGTAGGCCATGGTTGTTCACAGAAAGGAGAGGGCAGAACTCCTTGAAGCATTGGTTGCTCCAAATGATGTCACCATCTCTCAATATCCACAGGTTTTATAATTCTTTCCTTTTACTAAAAACAAAACAAAAATAGGTTGGAGCCATGCCTAATCTCCAAATGTTTGTGATTGGAAGTCAAAAGGTAACCTCAATTTTGAACACCAAACGTTCTATCCTCACCTTCGTATCAATTGAGCTAGACTCTTATTTACGTATGTCAATGAATAATTTTCTTCTTTTTGTTACTGTTATTTAACTCTTCTTATTTTAAAATTAGTTTTGATGTAACATTATAATGGAATTATAATAATATTCATTCACTTTATTTTTTTTTTTGCTTATTTTTCTTTGCAGAAGTTGCATATCATATGGGGGAGAAATGATAATTTGTTTGATATTCAAATTGCTTACAATATGAAGGAGTAAGTTATTTCTCATTCATTCTTATTTTAGTTTTTTTTTTTTTTCAAATTCAACAATTTTATTATTATTATTTTGAGTGTTGATGAATTATAATTGGTTCTACAATTTTATCAAATATATATTATAATCAAGATTTTGGTTTCTCAAACACGTTAATTCAATAAGAAAAAAATGGGTAGGAATCTCATTATATCAAACCCAAGCTTGAATATACTTATCATATCTACGTGGAAAAGTAATAAATGTGGTCTTACAAATTTCCTTAGCAGTAAAGTACTTTTCTATAATCATAATAATAATTATTATTATTTGGATTGATTGAATTTGAAATAATCATAATTTGGTGCAAAGGAAATTTGGAGAAAAAGCAACAATGGATTGCATAGAAAAAGCAGGCCATATTGTAGCAATGGAGCGCCCATTCATCTATAACAAATGCCTTCAAAAGTTTCTTCATTCACTGGAAGATAACTCCTACGAATGATCCATCTAATTATATAACCCTTCAAGTACTTTTATTTATTATATCTATAGTTGTATTTTAAGTTCAACCGTACTTTTCTAGTCTATATTATGTTTTTTTTCAATCATGAATTTTATTTATTGTCAAAGATATAATCCTGACAATCGATCATAGTTCATGTATAGTTTTTTATATAGTTTTACTTTGTCTCTAAATGCCAAAATATTACCCTTTGTCTCTAAGTTTTTAAATATTACATTATTGCAAGTTTTAACCAAGTTATAAAGTTAATTTCCCACTTAGATCATAGGCTTCA

Coding sequence (CDS)

ATGGTCAACATCTTCCACATCTACGACCAGGTTCTTCGTTGGGTAATGAAAGCAGCCGGAATCAAACCGGTTCAGCTTCAAATCAAACCCGGAACCGCCATGAACTTCTGGGTTCCCGCCGGAAAACCAAACAAAAACCACCAAAACCATCCTCCCCTCCTTTTCCTCCATGGGTTCGCCACCAATGCCATTATGACATGGCAATTCCAAGTCCTGAAGTTCGCCAAAAACCACGCCGTTTATGTACCGGACTTCATGTTCTTCGGGGATTCCGTTACGGATCGGCCGGACCGATCGACGGAGTTTCAGGCGGAGTGCGTGGTGGAGGGGTTGAGAAAATTGGGTGTGGACCGAAGGTTTGTTTTGGTGGGGTTTAGTTATGGGGCGATGGTGGGGTTTAGATTGGCGGAGATGTATCCGGAAATGGTGGAGGCGATGGTGGTTACGGCGGCGCCGACGGTGCTGACGGAAAGGATTACCGGAGAGGCAATGGAAAAAATAGGGTATAAGAGTTGGTCTGAATATTTGATACCGGAGACGGTGAAGGGTGCTATGTCTATGCTTGAAATTGCTTCCTTTGAATTTCCACGATTTCCACGTTGGATTTTCAAGCAATATCTGGAGGCCATGGTTGTTCACAGAAAGGAGAGGGCAGAACTCCTTGAAGCATTGGTTGCTCCAAATGATGTCACCATCTCTCAATATCCACAGGTTTTATAA

Protein sequence

MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL
Homology
BLAST of Cucsat.G4075.T2 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 48/184 (26.09%), Postives = 83/184 (45.11%), Query Frame = 0

Query: 26  LQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDF 85
           ++IK       +V AG+  K     P +L LHGF      +W+ Q+ +F   + V   D 
Sbjct: 72  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDL 131

Query: 86  MFFGDSVTDRPDRSTEFQAECVVEGLR----KLGVDRRFVLVGFSYGAMVGFRLAEMYPE 145
             +G+S  D P     ++ +C++  ++     LG   + VL+G  +G M+ + +A  YPE
Sbjct: 132 RGYGES--DAPAHQESYKLDCLIADIKDILDSLGYS-KCVLIGHDWGGMIAWLIAVCYPE 191

Query: 146 MVEAMVVT--AAPTVLTERITGEAMEKIGYKSWSEYLIPETVKGAMSMLEIASFEFPRFP 204
           M+  ++V     P+V TE I     +   ++S   Y                 F+ PRFP
Sbjct: 192 MIMKLIVINFPHPSVFTEYILRHPAQL--FRSSFYYF----------------FQIPRFP 228

BLAST of Cucsat.G4075.T2 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 3.4e-07
Identity = 41/140 (29.29%), Postives = 69/140 (49.29%), Query Frame = 0

Query: 26  LQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDF 85
           ++IK       +V AG+  K     P +L LHGF      +W++Q+ +F   + V   D 
Sbjct: 74  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDL 133

Query: 86  MFFGDSVTDRPDRSTEFQAECVVEGLR----KLGVDRRFVLVGFSYGAMVGFRLAEMYPE 145
             +G+  TD P     ++ +C++  ++     LG   + VL+G  +G M+ + +A  YPE
Sbjct: 134 RGYGE--TDAPIHRQNYKLDCLITDIKDILDSLGYS-KCVLIGHDWGGMIAWLIAICYPE 193

Query: 146 MVEAMVVT--AAPTVLTERI 160
           MV  ++V     P V TE I
Sbjct: 194 MVMKLIVINFPHPNVFTEYI 204

BLAST of Cucsat.G4075.T2 vs. ExPASy Swiss-Prot
Match: G5EBI4 (Epoxide hydrolase 1 OS=Caenorhabditis elegans OX=6239 GN=ceeh-1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.4e-06
Identity = 35/129 (27.13%), Postives = 65/129 (50.39%), Query Frame = 0

Query: 51  PPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEG 110
           P +LF+HG+      +W+FQ+ +FA  +     D    G +++D+P     +  + +   
Sbjct: 140 PLMLFIHGY-PEFWYSWRFQLKEFADKYRCVAIDQR--GYNLSDKPKHVDNYSIDELTGD 199

Query: 111 LRK----LGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEK 170
           +R     LG D+  ++V   +G +V ++ AE YPEMV+ ++    P        G   ++
Sbjct: 200 IRDVIEGLGYDKA-IVVAHDWGGLVAWQFAEQYPEMVDKLICCNIPR------PGSFRKR 257

Query: 171 IGYKSWSEY 176
           I Y SWS++
Sbjct: 260 I-YTSWSQF 257

BLAST of Cucsat.G4075.T2 vs. NCBI nr
Match: XP_004148865.1 (epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_006154 [Cucumis sativus])

HSP 1 Score: 480 bits (1236), Expect = 5.13e-170
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
           TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDR+TEFQAECVVEGLRKLGVDRRF
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           VKGA+SML+IASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQ L
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKL 239

BLAST of Cucsat.G4075.T2 vs. NCBI nr
Match: KAA0045542.1 (epoxide hydrolase 4-like [Cucumis melo var. makuwa] >TYK28850.1 epoxide hydrolase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 440 bits (1132), Expect = 3.71e-155
Identity = 213/238 (89.50%), Postives = 222/238 (93.28%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQVL
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQVL 240

BLAST of Cucsat.G4075.T2 vs. NCBI nr
Match: XP_016902619.1 (PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo])

HSP 1 Score: 438 bits (1126), Expect = 6.50e-154
Identity = 212/238 (89.08%), Postives = 221/238 (92.86%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQ L
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKL 240

BLAST of Cucsat.G4075.T2 vs. NCBI nr
Match: XP_038902558.1 (epoxide hydrolase 4-like [Benincasa hispida])

HSP 1 Score: 400 bits (1028), Expect = 2.36e-138
Identity = 192/239 (80.33%), Postives = 214/239 (89.54%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYD+V+RWVMKAAG++PVQ+QIKPGT MNFWVPAG PNK HQNHPPLL LHGFA
Sbjct: 1   MVNIFHIYDRVVRWVMKAAGVRPVQVQIKPGTTMNFWVPAGNPNKIHQNHPPLLLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            NAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRS EFQAECV EGLRKLGV+R  
Sbjct: 61  ANAIMTWQFQVLNLAKKYAVYVPDFIFFGESVTDRPDRSPEFQAECVAEGLRKLGVERGC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFR+AEMYPE+VEAMVVTAAPTVL E I+ E++EKIG+KSWSEYLIPE+
Sbjct: 121 VLVGFSYGAMVGFRMAEMYPELVEAMVVTAAPTVLMESISRESLEKIGFKSWSEYLIPES 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
            +G  S+LEIASF+FPR PRWIFK YLEAMV+H+KERAELL  LVA +DV ISQYPQ L
Sbjct: 181 AEGVKSLLEIASFKFPRVPRWIFKHYLEAMVMHKKERAELLRELVAADDVIISQYPQKL 239

BLAST of Cucsat.G4075.T2 vs. NCBI nr
Match: XP_023533135.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 340 bits (872), Expect = 1.29e-115
Identity = 162/238 (68.07%), Postives = 195/238 (81.93%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNP--NPPVVLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +RS EFQAECVV+GLRKLGV+R  
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRKLGVERPC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APT LTE ++  A+++IGYKSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDYLIPNT 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQV 238
           V G  S+ EI SF+FP  P WI K YLEAMV +RKE+AELL ALV P+D TISQ+PQV
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQV 236

BLAST of Cucsat.G4075.T2 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 480 bits (1236), Expect = 2.48e-170
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
           TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDR+TEFQAECVVEGLRKLGVDRRF
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           VKGA+SML+IASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQ L
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKL 239

BLAST of Cucsat.G4075.T2 vs. ExPASy TrEMBL
Match: A0A5A7TQB4 (Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00590 PE=4 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 1.79e-155
Identity = 213/238 (89.50%), Postives = 222/238 (93.28%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQVL
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQVL 240

BLAST of Cucsat.G4075.T2 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 3.15e-154
Identity = 212/238 (89.08%), Postives = 221/238 (92.86%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQ L
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKL 240

BLAST of Cucsat.G4075.T2 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 333 bits (854), Expect = 3.11e-112
Identity = 160/237 (67.51%), Postives = 192/237 (81.01%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNR--NPPVVLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +RS EFQAE VV+GLRKLGV+R  
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APT LTE ++  A+ +IGYKSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNT 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQ 237
           V G  S+ EI SF+FP  P WI K YLEAMV +RKE+AELL ALV P+D TISQ+PQ
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQ 235

BLAST of Cucsat.G4075.T2 vs. ExPASy TrEMBL
Match: A0A6J1KWX2 (epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1)

HSP 1 Score: 301 bits (771), Expect = 5.55e-101
Identity = 140/208 (67.31%), Postives = 173/208 (83.17%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNP--NPPVVLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +RS EFQAECVV+GLR+LGV+R  
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APTVLTE ++ EA+++IGYKSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNT 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLE 208
           VKG  S+ EI SF+FP  P WI +QY++
Sbjct: 181 VKGVKSLFEIGSFDFPALPNWIMEQYVK 206

BLAST of Cucsat.G4075.T2 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 2.5e-58
Identity = 115/250 (46.00%), Postives = 166/250 (66.40%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVP---AGKPNKNHQN-------- 60
           MVN   +   +L  +MK AG+ P  ++++PGT MNFW+P     K  K+ +N        
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 61  -HPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVV 120
             P LLF+HGFA   I+TWQFQV   AK ++VY+PD +FFG S +D  DRS  FQA C+V
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 121 EGLRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIG 180
           + LR LG++ +F LVGFSYG MV F++AE YPEMV+AMVV+ +   +T+ I+   + ++G
Sbjct: 121 KSLRILGIE-KFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLG 180

Query: 181 YKSWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPN 238
           +KS ++ L+P +VKG  ++  +A  +   FP+ +FK ++E M+ +RKERAELLEALV  N
Sbjct: 181 FKSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISN 240

BLAST of Cucsat.G4075.T2 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 1.8e-56
Identity = 113/248 (45.56%), Postives = 161/248 (64.92%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVP---------AGKPNK-NHQNH 60
           MVN   +   +L  +MK AG+ P  L+I+PGT +NFWVP          GKP K +    
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 61  PPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEG 120
           P +L +HGFA   I+TWQFQV   +K ++VY+PD +FFG S TD  DRS  FQA+C+V+G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 121 LRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYK 180
           LR LGVD +FV VGFSYG MV F++AE YP+MV A+VV+ +   +T+ I   ++ ++G+ 
Sbjct: 121 LRILGVD-KFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFS 180

Query: 181 SWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPN-D 238
           S ++ L+P +V G  ++  IA  +   FP+ +FK Y+E M  +RKERAELLEA+V  N +
Sbjct: 181 SSTDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKE 240

BLAST of Cucsat.G4075.T2 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 6.5e-30
Identity = 76/229 (33.19%), Postives = 122/229 (53.28%), Query Frame = 0

Query: 17  KAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAK 76
           K +G++PV + +K GT +NFWV   KP    +  P LL +HG    AI  W     + ++
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWVSKTKPESKPK--PNLLLIHGLGATAIWQWYDVARRLSR 79

Query: 77  NHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAMVGFRLA 136
              +Y+PD +FFG S T RP+RS  FQA+ ++  L    V ++F LVG SYG  VG+R+A
Sbjct: 80  YFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSV-KKFSLVGLSYGGFVGYRMA 139

Query: 137 EMYPEMVEAMVVTAAPTVLTERITGEAMEKIG-YKSWSEYLIPETVKGAMSMLEIASFEF 196
            MY + VE +V+  A   + E+     + K+      S+ L+PE+VK    ++    ++ 
Sbjct: 140 SMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYK- 199

Query: 197 PRFPRWIFKQYLEAMVVH------RKERAELLEALVAPNDVTISQYPQV 239
           P   R +    L   + H       +E+ EL++A+  P D  IS+ P++
Sbjct: 200 PALARLVPTCLLHDFIEHALTRDNMEEKRELIKAI--PKDRIISEIPKL 242

BLAST of Cucsat.G4075.T2 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 3.7e-25
Identity = 69/209 (33.01%), Postives = 107/209 (51.20%), Query Frame = 0

Query: 19  AGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNH 78
           AG++     +  GT  + W+P      +    P LL LHG   NA+  W   + +F    
Sbjct: 22  AGLRSSTSDLGDGTVFHCWIPL----THIHTKPTLLLLHGIGANAMWQWDRFIDRFIPRF 81

Query: 79  AVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAMVGFRLAEM 138
            VYVPD +FFGDS T RPDRS  FQA CV++ +   GV R   + G SYG  V + LA  
Sbjct: 82  NVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGV-RTMTVAGLSYGGFVAYSLAAQ 141

Query: 139 YPEMVEAMVVTAAPTVLTERITGEAMEKI-GYKSWSEYLIPETVKGAMSMLEIASFEFPR 198
           + E V+ +V+  A   L E+ + + M K+   +  +  L P++      +L+++ ++ P 
Sbjct: 142 FKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPSMLRRLLQLSFYKPPI 201

Query: 199 F-PRWIFKQYLEAMVV-HRKERAELLEAL 225
           + P      Y+  M   + +ER EL+EAL
Sbjct: 202 WIPSCFAMDYIHVMCKDYLQERKELVEAL 225

BLAST of Cucsat.G4075.T2 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 4.1e-24
Identity = 65/218 (29.82%), Postives = 124/218 (56.88%), Query Frame = 0

Query: 12  LRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQV 71
           LR  ++AAG+    L I   T ++FW P    +++  + P +L LHGF  +++  W+ Q+
Sbjct: 17  LRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQWRRQM 76

Query: 72  LKFAKN-HAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAM 131
             F+ +   VY PD +FFGDS +   +R+  FQAEC+ + + K+G+  ++ + G SYG  
Sbjct: 77  QAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIG-KYNVAGTSYGGF 136

Query: 132 VGFRLAEMYPEMVEAMVVTAAPTVLTERITGEA-MEKIGYKSWSEYLIPETVKGAMSMLE 191
           V + +A+M+PE VE +V+ A+  +   +  GE+ +++   +   + ++P T     +++ 
Sbjct: 137 VAYHMAKMWPEKVEKVVI-ASSGINMRKCDGESLLQRSNCECIEKVMLPSTATEFRTLMA 196

Query: 192 IA-SFEFPR-FPRWIFKQYLEAMV-VHRKERAELLEAL 225
           +A S+   R FP  ++   +  +   +RKE+ ELL+ +
Sbjct: 197 LASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGV 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6IE262.0e-0726.09Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
Q8IUS53.4e-0729.29Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2[more]
G5EBI48.4e-0627.13Epoxide hydrolase 1 OS=Caenorhabditis elegans OX=6239 GN=ceeh-1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004148865.15.13e-17098.33epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_00615... [more]
KAA0045542.13.71e-15589.50epoxide hydrolase 4-like [Cucumis melo var. makuwa] >TYK28850.1 epoxide hydrolas... [more]
XP_016902619.16.50e-15489.08PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo][more]
XP_038902558.12.36e-13880.33epoxide hydrolase 4-like [Benincasa hispida][more]
XP_023533135.11.29e-11568.07epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LLY62.48e-17098.33AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247... [more]
A0A5A7TQB41.79e-15589.50Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S4E3103.15e-15489.08uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=... [more]
A0A6J1G8063.11e-11267.51epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1[more]
A0A6J1KWX25.55e-10167.31epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G18360.12.5e-5846.00alpha/beta-Hydrolases superfamily protein [more]
AT4G36610.11.8e-5645.56alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.16.5e-3033.19alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.13.7e-2533.01alpha/beta-Hydrolases superfamily protein [more]
AT4G33180.14.1e-2429.82alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 51..165
e-value: 3.6E-14
score: 53.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..237
e-value: 7.2E-30
score: 106.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 43..228
NoneNo IPR availablePANTHERPTHR43139:SF22EPOXIDE HYDROLASE 4-LIKEcoord: 2..238
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 2..238

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G4075Cucsat.G4075gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G4075.T2.E1Cucsat.G4075.T2.E1exon
Cucsat.G4075.T2.E2Cucsat.G4075.T2.E2exon
Cucsat.G4075.T2.E3Cucsat.G4075.T2.E3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G4075.T2.C2Cucsat.G4075.T2.C2CDS
Cucsat.G4075.T2.C1Cucsat.G4075.T2.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G4075.T2Cucsat.G4075.T2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity