Cucsat.G4075 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4075
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAB hydrolase-1 domain-containing protein
Locationctg105: 3643811 .. 3646489 (-)
RNA-Seq ExpressionCucsat.G4075
SyntenyCucsat.G4075
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAATAGGAAAAGAAGCCATGCAGTAGAAATTTTTTTGTTCAATTATTTCATCATACATGACACCTGTCCAAACGTGTCTCTCAAAGTTATTTAATGCTAGTAAAACCATTTGTTTTCTTCACAATTCCTCCGATTTTTTCTTCCTTGAATACACAAAAGCTTAAGTTCAAAGCAACTCATCAATGGTCAACATCTTCCACATCTACGACCAGGTTCTTCGTTGGGTAATGAAAGCAGCCGGAATCAAACCGGTTCAGCTTCAAATCAAACCCGGAACCGCCATGAACTTCTGGGTTCCCGCCGGAAAACCAAACAAAAACCACCAAAACCATCCTCCCCTCCTTTTCCTCCATGGGTTCGCCACCAATGCCATTATGACATGGCAATTCCAAGTCCTGAAGTTCGCCAAAAACCACGCCGTTTATGTACCGGACTTCATGTTCTTCGGGGATTCCGTTACGGATCGGCCGGACCGATCGACGGAGTTTCAGGCGGAGTGCGTGGTGGAGGGGTTGAGAAAATTGGGTGTGGACCGAAGGTTTGTTTTGGTGGGGTTTAGTTATGGGGCGATGGTGGGGTTTAGATTGGCGGAGATGTATCCGGAAATGGTGGAGGCGATGGTGGTTACGGCGGCGCCGACGGTGCTGACGGAAAGGATTACCGGAGAGGCAATGGAAAAAATAGGGTATAAGAGTTGGTCTGAATATTTGATACCGGAGACGGTGAAGGGTGCTATGTCTATGCTTGAAATTGCTTCCTTTGAATTTCCACGATTTCCACGTTGGATTTTCAAGCAATATCTGGAGGTACGTCAAAATTATTTTTGTAAAACCATTTCTTTTTTATTTATTTATTTTCCTTACGGAGAATTCAATGGTAAATAACCATAGATTGAAAAAAAAACATTTTTTTTGTGGGATATGGTTTTTAAAAGTTATTCTACTCATTTAACTATTTTATTTAAAATAAAAGTATAAAGTTATTTTTAAAAAACATTTTGAAACTCAAAATTCCCATTTTTAAAACGTATAGATCAAACCTATATATTCATCAAAACTTACAAACTAAAAAGATTAATATTTTTAAATATTTATTAACACTAGACAAGGTTGAATGTCATTAATATCGCAAATATTTAGGTCCTTTTGAAAACAAGTTTTGTGAAAAACTCAAGCTCTTAGTTAAATTCTAAAATCCCAAAACTTGCATTTAAAAAACCAAAACATGCAAACAAATAATTAACAAGTGAGTGCAATCAGATCAAAAATTGGATTCATTATTATTTTCTAGAATATAAAGAGTCATTTACGATAATTATTTGTAATTTTTTAAAAATGTTATTATATATTTAATTATTTTAAAATTAAATTATAAGGTAGAGTAGAGACCCATAAAGTTGCCATGCGTGCAGTTATTAGAGACGACCTCAGCAGATTTATAATATACATTTTTGTACATTATTATTGATGATGGGAAAACTGATTTGAAATTAGTATTTTGTTTTGTTAATAAAAGGAAAAGGATGGTTGTGATTGTAGGCCATGGTTGTTCACAGAAAGGAGAGGGCAGAACTCCTTGAAGCATTGGTTGCTCCAAATGATGTCACCATCTCTCAATATCCACAGGTTTTATAATTCTTTCCTTTTACTAAAAACAAAACAAAAATAGGTTGGAGCCATGCCTAATCTCCAAATGTTTGTGATTGGAAGTCAAAAGGTAACCTCAATTTTGAACACCAAACGTTCTATCCTCACCTTCGTATCAATTGAGCTAGACTCTTATTTACGTATGTCAATGAATAATTTTCTTCTTTTTGTTACTGTTATTTAACTCTTCTTATTTTAAAATTAGTTTTGATGTAACATTATAATGGAATTATAATAATATTCATTCACTTTATTTTTTTTTTTGCTTATTTTTCTTTGCAGAAGTTGCATATCATATGGGGGAGAAATGATAATTTGTTTGATATTCAAATTGCTTACAATATGAAGGAGTAAGTTATTTCTCATTCATTCTTATTTTAGTTTTTTTTTTTTTTCAAATTCAACAATTTTATTATTATTATTTTGAGTGTTGATGAATTATAATTGGTTCTACAATTTTATCAAATATATATTATAATCAAGATTTTGGTTTCTCAAACACGTTAATTCAATAAGAAAAAAATGGGTAGGAATCTCATTATATCAAACCCAAGCTTGAATATACTTATCATATCTACGTGGAAAAGTAATAAATGTGGTCTTACAAATTTCCTTAGCAGTAAAGTACTTTTCTATAATCATAATAATAATTATTATTATTTGGATTGATTGAATTTGAAATAATCATAATTTGGTGCAAAGGAAATTTGGAGAAAAAGCAACAATGGATTGCATAGAAAAAGCAGGCCATATTGTAGCAATGGAGCGCCCATTCATCTATAACAAATGCCTTCAAAAGTTTCTTCATTCACTGGAAGATAACTCCTACGAATGATCCATCTAATTATATAACCCTTCAAGTACTTTTATTTATTATATCTATAGTTGTATTTTAAGTTCAACCGTACTTTTCTAGTCTATATTATGTTTTTTTTCAATCATGAATTTTATTTATTGTCAAAGATATAATCCTGACAATCGATCATAGTTCATGTATAGTTTTTTATATAGTTTTACTTTGTCTCTAAATGCCAAAATATTACCCTTTGTCTCTAAGTTTTTAAATATTACATTATTGCAAGTTTTAACCAAGTTATAAAGTTAATTTCCCACTTAGATCATAGGCTTCA

Coding sequence (CDS)

ATGGTCAACATCTTCCACATCTACGACCAGGTTCTTCGTTGGGTAATGAAAGCAGCCGGAATCAAACCGGTTCAGCTTCAAATCAAACCCGGAACCGCCATGAACTTCTGGGTTCCCGCCGGAAAACCAAACAAAAACCACCAAAACCATCCTCCCCTCCTTTTCCTCCATGGGTTCGCCACCAATGCCATTATGACATGGCAATTCCAAGTCCTGAAGTTCGCCAAAAACCACGCCGTTTATGTACCGGACTTCATGTTCTTCGGGGATTCCGTTACGGATCGGCCGGACCGATCGACGGAGTTTCAGGCGGAGTGCGTGGTGGAGGGGTTGAGAAAATTGGGTGTGGACCGAAGGTTTGTTTTGGTGGGGTTTAGTTATGGGGCGATGGTGGGGTTTAGATTGGCGGAGATGTATCCGGAAATGGTGGAGGCGATGGTGGTTACGGCGGCGCCGACGGTGCTGACGGAAAGGATTACCGGAGAGGCAATGGAAAAAATAGGGTATAAGAGTTGGTCTGAATATTTGATACCGGAGACGGTGAAGGGTGCTATGTCTATGCTTGAAATTGCTTCCTTTGAATTTCCACGATTTCCACGTTGGATTTTCAAGCAATATCTGGAGGCCATGGTTGTTCACAGAAAGGAGAGGGCAGAACTCCTTGAAGCATTGGTTGCTCCAAATGATGTCACCATCTCTCAATATCCACAGGTTTTATAA

Protein sequence

MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQVL
Homology
BLAST of Cucsat.G4075 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 68/291 (23.37%), Postives = 121/291 (41.58%), Query Frame = 0

Query: 26  LQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDF 85
           ++IK       +V AG+  K     P +L LHGF      +W++Q+ +F   + V   D 
Sbjct: 74  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDL 133

Query: 86  MFFGDSVTDRPDRSTEFQAECVVEGLR----KLGVDRRFVLVGFSYGAMVGFRLAEMYPE 145
             +G+  TD P     ++ +C++  ++     LG   + VL+G  +G M+ + +A  YPE
Sbjct: 134 RGYGE--TDAPIHRQNYKLDCLITDIKDILDSLGYS-KCVLIGHDWGGMIAWLIAICYPE 193

Query: 146 MVEAMVVT--AAPTVLTERITGEAMEKIGYKSWSEYLIP---------ETVKGAMSMLEI 205
           MV  ++V     P V TE I     + +    +  + IP            K    +   
Sbjct: 194 MVMKLIVINFPHPNVFTEYILRHPAQLLKSSYYYFFQIPWFPEFMFSINDFKVLKHLFTS 253

Query: 206 ASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH-------IIW 265
            S    R    +  + LEA +    +   L   +    ++  S  P K H       ++W
Sbjct: 254 HSTGIGRKGCQLTTEDLEAYIYVFSQPGALSGPINHYRNI-FSCLPLKHHMVTTPTLLLW 313

Query: 266 GRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFL 295
           G ND   ++++A   K        +  + +A H +  ++P I NK +  FL
Sbjct: 314 GENDAFMEVEMAEVTKIYVKNYFRLTILSEASHWLQQDQPDIVNKLIWTFL 354

BLAST of Cucsat.G4075 vs. ExPASy Swiss-Prot
Match: G5EBI4 (Epoxide hydrolase 1 OS=Caenorhabditis elegans OX=6239 GN=ceeh-1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-10
Identity = 71/290 (24.48%), Postives = 122/290 (42.07%), Query Frame = 0

Query: 51  PPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEG 110
           P +LF+HG+      +W+FQ+ +FA  +     D    G +++D+P     +  + +   
Sbjct: 140 PLMLFIHGY-PEFWYSWRFQLKEFADKYRCVAIDQR--GYNLSDKPKHVDNYSIDELTGD 199

Query: 111 LRK----LGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEK 170
           +R     LG D+  ++V   +G +V ++ AE YPEMV+ ++    P        G   ++
Sbjct: 200 IRDVIEGLGYDKA-IVVAHDWGGLVAWQFAEQYPEMVDKLICCNIPR------PGSFRKR 259

Query: 171 IGYKSWSEY------------LIPETVKGA--MSMLEIA------------SFEFPRFPR 230
           I Y SWS++             IPE +  A  M MLE+             +F       
Sbjct: 260 I-YTSWSQFRKSWYMFFYQNEKIPEMLCSADDMKMLELCFRAKEIGIQNNKNFTDEDLEA 319

Query: 231 WIFK------------QYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLHIIWGRNDN 290
           W +              Y   +   +K++A+L+  L  P             IIWG  D 
Sbjct: 320 WKYSFSMNGASFKYPINYYRNIFNAKKQQADLV--LEMPT-----------LIIWGTADG 379

Query: 291 LFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLE 299
             DI+ A +      ++ TM  IE A H V  + P + N+ ++KFL+  +
Sbjct: 380 ALDIEAAVDSLNTL-KQGTMKKIEGASHWVQQDEPEMVNEHIKKFLNKYQ 404

BLAST of Cucsat.G4075 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 9.3e-10
Identity = 66/291 (22.68%), Postives = 118/291 (40.55%), Query Frame = 0

Query: 26  LQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDF 85
           ++IK       +V AG+  K     P +L LHGF      +W+ Q+ +F   + V   D 
Sbjct: 72  VRIKDSGLRFHYVAAGERGK-----PLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDL 131

Query: 86  MFFGDSVTDRPDRSTEFQAECVVEGLR----KLGVDRRFVLVGFSYGAMVGFRLAEMYPE 145
             +G+S  D P     ++ +C++  ++     LG   + VL+G  +G M+ + +A  YPE
Sbjct: 132 RGYGES--DAPAHQESYKLDCLIADIKDILDSLGYS-KCVLIGHDWGGMIAWLIAVCYPE 191

Query: 146 MVEAMVVT--AAPTVLTERITGEAMEKIGYKSWSEYLIP---------ETVKGAMSMLEI 205
           M+  ++V     P+V TE I     +      +  + IP            K    +   
Sbjct: 192 MIMKLIVINFPHPSVFTEYILRHPAQLFRSSFYYFFQIPRFPEFMFSINDFKALKHLFTS 251

Query: 206 ASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH-------IIW 265
            S    R  R +  + LEA V    +   L   +    ++  S  P K H       ++W
Sbjct: 252 QSTGIGRKGRQLTTEDLEAYVYVFSQPGALSGPINHYRNI-FSCLPLKHHMVTTPTLLLW 311

Query: 266 GRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFL 295
           G  D   ++++A   K        +  + +  H +  ++P I N  +  FL
Sbjct: 312 GEEDAFMEVEMAEVTKIYVKNYFRLTILSEGSHWLQQDQPDIVNGLIWAFL 352

BLAST of Cucsat.G4075 vs. NCBI nr
Match: XP_004148865.1 (epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_006154 [Cucumis sativus])

HSP 1 Score: 616 bits (1589), Expect = 1.08e-222
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
           TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDR+TEFQAECVVEGLRKLGVDRRF
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240
           VKGA+SML+IASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240

Query: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN 300
           IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN
Sbjct: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN 300

Query: 301 SYE 303
            YE
Sbjct: 301 PYE 303

BLAST of Cucsat.G4075 vs. NCBI nr
Match: XP_038902558.1 (epoxide hydrolase 4-like [Benincasa hispida])

HSP 1 Score: 509 bits (1311), Expect = 2.43e-180
Identity = 241/297 (81.14%), Postives = 268/297 (90.24%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYD+V+RWVMKAAG++PVQ+QIKPGT MNFWVPAG PNK HQNHPPLL LHGFA
Sbjct: 1   MVNIFHIYDRVVRWVMKAAGVRPVQVQIKPGTTMNFWVPAGNPNKIHQNHPPLLLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            NAIMTWQFQVL  AK +AVYVPDF+FFG+SVTDRPDRS EFQAECV EGLRKLGV+R  
Sbjct: 61  ANAIMTWQFQVLNLAKKYAVYVPDFIFFGESVTDRPDRSPEFQAECVAEGLRKLGVERGC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFR+AEMYPE+VEAMVVTAAPTVL E I+ E++EKIG+KSWSEYLIPE+
Sbjct: 121 VLVGFSYGAMVGFRMAEMYPELVEAMVVTAAPTVLMESISRESLEKIGFKSWSEYLIPES 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240
            +G  S+LEIASF+FPR PRWIFK YLEAMV+H+KERAELL  LVA +DV ISQYPQKLH
Sbjct: 181 AEGVKSLLEIASFKFPRVPRWIFKHYLEAMVMHKKERAELLRELVAADDVIISQYPQKLH 240

Query: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSL 297
           +IWGRNDNLFD+QIAYNM E+FG KA +DCIEKAGHIV +ERPFIYNKCLQ FLHSL
Sbjct: 241 VIWGRNDNLFDVQIAYNMIEEFGGKARLDCIEKAGHIVPLERPFIYNKCLQNFLHSL 297

BLAST of Cucsat.G4075 vs. NCBI nr
Match: XP_016902619.1 (PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo])

HSP 1 Score: 486 bits (1251), Expect = 7.53e-172
Identity = 234/259 (90.35%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLHI 241
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQKLHI
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKLHI 242

Query: 242 IWGRNDNLFDIQIAYNMKE 260
           IWGRNDNLFDIQIAYNMKE
Sbjct: 243 IWGRNDNLFDIQIAYNMKE 261

BLAST of Cucsat.G4075 vs. NCBI nr
Match: KAA0045542.1 (epoxide hydrolase 4-like [Cucumis melo var. makuwa] >TYK28850.1 epoxide hydrolase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 438 bits (1126), Expect = 3.85e-153
Identity = 212/238 (89.08%), Postives = 221/238 (92.86%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQ L
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQVL 240

BLAST of Cucsat.G4075 vs. NCBI nr
Match: XP_022947943.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 434 bits (1117), Expect = 7.81e-151
Identity = 207/298 (69.46%), Postives = 247/298 (82.89%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNR--NPPVVLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +RS EFQAE VV+GLRKLGV+R  
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APT LTE ++  A+ +IGYKSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNT 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240
           V G  S+ EI SF+FP  P WI K YLEAMV +RKE+AELL ALV P+D TISQ+PQK+H
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQKMH 240

Query: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLE 298
           IIWGRNDNLF++QIAYNMKE+FG +  +DCIEKAGHIV MERPFIYNK L+KFL SL+
Sbjct: 241 IIWGRNDNLFNMQIAYNMKEQFGGETRLDCIEKAGHIVPMERPFIYNKYLKKFLRSLQ 296

BLAST of Cucsat.G4075 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 616 bits (1589), Expect = 5.23e-223
Identity = 299/303 (98.68%), Postives = 302/303 (99.67%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
           TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDR+TEFQAECVVEGLRKLGVDRRF
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240
           VKGA+SML+IASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH
Sbjct: 181 VKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240

Query: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN 300
           IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN
Sbjct: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLEDN 300

Query: 301 SYE 303
            YE
Sbjct: 301 PYE 303

BLAST of Cucsat.G4075 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 486 bits (1251), Expect = 3.64e-172
Identity = 234/259 (90.35%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLHI 241
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQKLHI
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQKLHI 242

Query: 242 IWGRNDNLFDIQIAYNMKE 260
           IWGRNDNLFDIQIAYNMKE
Sbjct: 243 IWGRNDNLFDIQIAYNMKE 261

BLAST of Cucsat.G4075 vs. ExPASy TrEMBL
Match: A0A5A7TQB4 (Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00590 PE=4 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 1.87e-153
Identity = 212/238 (89.08%), Postives = 221/238 (92.86%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +NIFHIYD VLRWVMKAAGI PVQLQI+PGT+MNFWVPA KPNKNHQNHPPLLFLHGFA 
Sbjct: 3   INIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFAA 62

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           NAIMTWQFQVLKFAK HAVYVPDFMFFGDSVTDRPDRS EFQAECV EGLRKLGV+RRFV
Sbjct: 63  NAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRFV 122

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           LVGFSYGAMVGFRLAE YPE+VEAMVVTAAPTVLTERIT  A+EKIGYKSWSEYLIPETV
Sbjct: 123 LVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPETV 182

Query: 182 KGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKL 239
           KG  SMLEIASFEFPRFPRWIFK YLE M++H KERAELLEALV PNDVTIS+YPQ L
Sbjct: 183 KGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQVL 240

BLAST of Cucsat.G4075 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 434 bits (1117), Expect = 3.78e-151
Identity = 207/298 (69.46%), Postives = 247/298 (82.89%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60
           M NIFH+Y +VLRW+MK AG++   +QI+PGT MNFWVP   P KN   +PP++ LHGFA
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNR--NPPVVLLHGFA 60

Query: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRF 120
            N IMTWQFQV+  A+N+AVYVPDF+FFG+SVTDR +RS EFQAE VV+GLRKLGV+R  
Sbjct: 61  GNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPC 120

Query: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180
           VLVGFSYGAMVGFRLAEMYP++V AMVVT+APT LTE ++  A+ +IGYKSWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNT 180

Query: 181 VKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQKLH 240
           V G  S+ EI SF+FP  P WI K YLEAMV +RKE+AELL ALV P+D TISQ+PQK+H
Sbjct: 181 VNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQKMH 240

Query: 241 IIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSLE 298
           IIWGRNDNLF++QIAYNMKE+FG +  +DCIEKAGHIV MERPFIYNK L+KFL SL+
Sbjct: 241 IIWGRNDNLFNMQIAYNMKEQFGGETRLDCIEKAGHIVPMERPFIYNKYLKKFLRSLQ 296

BLAST of Cucsat.G4075 vs. ExPASy TrEMBL
Match: A0A7N2R8U7 (AB hydrolase-1 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 340 bits (872), Expect = 9.28e-114
Identity = 159/300 (53.00%), Postives = 227/300 (75.67%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKP--NKNHQNHPPLLFLHG 60
           MVNIF +Y  +L WVMK  G++P +++I+PGT MNFWVP   P  +KN  N+  ++F+HG
Sbjct: 1   MVNIFAVYKPLLHWVMKLVGVRPQRVEIEPGTIMNFWVPKETPKKSKNSNNNKAVVFIHG 60

Query: 61  FATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDR 120
           FA + I+TWQ QVL  A+ + VYVPD +FFG S+TDR +RS EFQAECV +GL+KLGV+R
Sbjct: 61  FADDGIITWQSQVLALARKYKVYVPDLVFFGGSITDRSERSPEFQAECVAKGLKKLGVER 120

Query: 121 RFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIP 180
              LVG SYG MVGF++AEMYP++VE+MV+T +   LT+ I+  A+E+IG+KSWS+YL+P
Sbjct: 121 -CTLVGLSYGGMVGFKMAEMYPDLVESMVLTCSVMALTKSISDAALERIGFKSWSDYLLP 180

Query: 181 ETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAP-NDVTISQYPQ 240
           ++V+G   + +IA+F+ PR P +I+K +LE M  +RKER ELLEALV   ND TI  +PQ
Sbjct: 181 DSVEGVKILFDIATFKLPRIPNFIYKHHLEIMFDNRKEREELLEALVIDDNDFTIPSHPQ 240

Query: 241 KLHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSL 297
           ++H++WG ND +F++++  N+K + G++AT+  IEKAGH+V  ERP +YNK L++ L +L
Sbjct: 241 RVHLLWGENDIIFNMEVVNNLKRQLGDRATLQYIEKAGHLVEAERPCVYNKHLKEILTTL 299

BLAST of Cucsat.G4075 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.0e-80
Identity = 145/310 (46.77%), Postives = 214/310 (69.03%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVP---AGKPNKNHQN-------- 60
           MVN   +   +L  +MK AG+ P  ++++PGT MNFW+P     K  K+ +N        
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 61  -HPPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVV 120
             P LLF+HGFA   I+TWQFQV   AK ++VY+PD +FFG S +D  DRS  FQA C+V
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 121 EGLRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIG 180
           + LR LG++ +F LVGFSYG MV F++AE YPEMV+AMVV+ +   +T+ I+   + ++G
Sbjct: 121 KSLRILGIE-KFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLG 180

Query: 181 YKSWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPN 240
           +KS ++ L+P +VKG  ++  +A  +   FP+ +FK ++E M+ +RKERAELLEALV  N
Sbjct: 181 FKSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISN 240

Query: 241 -DVTISQYPQKLHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYN 298
            DVTI ++ QK+H++WG +D +F+++ A +MKE+ GE ATM+ I+KAGH+  +ERP +YN
Sbjct: 241 KDVTIPRFQQKIHLLWGESDQIFNLEFAKSMKEQLGENATMESIKKAGHLAHLERPCVYN 300

BLAST of Cucsat.G4075 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.3e-79
Identity = 144/308 (46.75%), Postives = 210/308 (68.18%), Query Frame = 0

Query: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVP---------AGKPNK-NHQNH 60
           MVN   +   +L  +MK AG+ P  L+I+PGT +NFWVP          GKP K +    
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 61  PPLLFLHGFATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEG 120
           P +L +HGFA   I+TWQFQV   +K ++VY+PD +FFG S TD  DRS  FQA+C+V+G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 121 LRKLGVDRRFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYK 180
           LR LGVD +FV VGFSYG MV F++AE YP+MV A+VV+ +   +T+ I   ++ ++G+ 
Sbjct: 121 LRILGVD-KFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFS 180

Query: 181 SWSEYLIPETVKGAMSMLEIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPN-D 240
           S ++ L+P +V G  ++  IA  +   FP+ +FK Y+E M  +RKERAELLEA+V  N +
Sbjct: 181 SSTDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKE 240

Query: 241 VTISQYPQKLHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKC 298
             I  +P+K+H +WG +D +FD+++A +MKE+ GE AT++ I+KAGH+V +ERP +YN+ 
Sbjct: 241 AQIPHFPRKIHFLWGESDQIFDLELARDMKEQIGENATIESIKKAGHLVQLERPCVYNRR 300

BLAST of Cucsat.G4075 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 6.7e-40
Identity = 94/288 (32.64%), Postives = 153/288 (53.12%), Query Frame = 0

Query: 17  KAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQVLKFAK 76
           K +G++PV + +K GT +NFWV   KP    +  P LL +HG    AI  W     + ++
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWVSKTKPESKPK--PNLLLIHGLGATAIWQWYDVARRLSR 79

Query: 77  NHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAMVGFRLA 136
              +Y+PD +FFG S T RP+RS  FQA+ ++  L    V ++F LVG SYG  VG+R+A
Sbjct: 80  YFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSV-KKFSLVGLSYGGFVGYRMA 139

Query: 137 EMYPEMVEAMVVTAAPTVLTERITGEAMEKIG-YKSWSEYLIPETVKGAMSMLEIASFEF 196
            MY + VE +V+  A   + E+     + K+      S+ L+PE+VK    ++    ++ 
Sbjct: 140 SMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYK- 199

Query: 197 PRFPRWIFKQYLEAMVVH------RKERAELLEALVAPNDVTISQYP---QKLHIIWGRN 256
           P   R +    L   + H       +E+ EL++A+  P D  IS+ P   Q   IIWG +
Sbjct: 200 PALARLVPTCLLHDFIEHALTRDNMEEKRELIKAI--PKDRIISEIPKLKQPTLIIWGEH 259

Query: 257 DNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFL 295
           D +F +++   +++  G+   +  I++ GHI   E+P  + K L+ FL
Sbjct: 260 DQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFL 301

BLAST of Cucsat.G4075 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 9.1e-37
Identity = 90/297 (30.30%), Postives = 148/297 (49.83%), Query Frame = 0

Query: 2   VNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFAT 61
           +++    + +LR    +AG+    L I   T + FW P    +  +   P LL LHGF  
Sbjct: 6   LSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFGP 65

Query: 62  NAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFV 121
           +A+  W  QV   +    +YVPD +FFG S +   +RS  FQA C+ + + KL V+ RF 
Sbjct: 66  SAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVE-RFS 125

Query: 122 LVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPETV 181
           +VG SYG  V + +A+M+PE VE +V+ ++   L        + +       E ++P + 
Sbjct: 126 VVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPASA 185

Query: 182 KGAMSML-EIASFEFPRFPRWIFKQYLEAMVVH-RKERAELLEALVAPND--VTISQYPQ 241
                    ++S      P ++   + + M    R+E+AELLE L    D    +S   Q
Sbjct: 186 TDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSPIQQ 245

Query: 242 KLHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFL 295
            + +IWG  D +F +++A+++KE  G KAT+  I+K  HI   E+   +N  +  FL
Sbjct: 246 DVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFL 301

BLAST of Cucsat.G4075 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.5e-36
Identity = 84/290 (28.97%), Postives = 162/290 (55.86%), Query Frame = 0

Query: 12  LRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFATNAIMTWQFQV 71
           LR  ++AAG+    L I   T ++FW P    +++  + P +L LHGF  +++  W+ Q+
Sbjct: 17  LRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQWRRQM 76

Query: 72  LKFAKN-HAVYVPDFMFFGDSVTDRPDRSTEFQAECVVEGLRKLGVDRRFVLVGFSYGAM 131
             F+ +   VY PD +FFGDS +   +R+  FQAEC+ + + K+G+  ++ + G SYG  
Sbjct: 77  QAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIG-KYNVAGTSYGGF 136

Query: 132 VGFRLAEMYPEMVEAMVVTAAPTVLTERITGEA-MEKIGYKSWSEYLIPETVKGAMSMLE 191
           V + +A+M+PE VE +V+ A+  +   +  GE+ +++   +   + ++P T     +++ 
Sbjct: 137 VAYHMAKMWPEKVEKVVI-ASSGINMRKCDGESLLQRSNCECIEKVMLPSTATEFRTLMA 196

Query: 192 IA-SFEFPR-FPRWIFKQYLEAMV-VHRKERAELLEALV--APNDVTISQYPQKLHIIWG 251
           +A S+   R FP  ++   +  +   +RKE+ ELL+ +      ++ I    Q++ I+WG
Sbjct: 197 LASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVLIVWG 256

Query: 252 RNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFL 295
             D +F +++AY +KE  G+K  ++ I+   H+  +E    +N  + +FL
Sbjct: 257 DKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8IUS51.1e-1023.37Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2[more]
G5EBI41.9e-1024.48Epoxide hydrolase 1 OS=Caenorhabditis elegans OX=6239 GN=ceeh-1 PE=1 SV=1[more]
Q6IE269.3e-1022.68Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_004148865.11.08e-22298.68epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_00615... [more]
XP_038902558.12.43e-18081.14epoxide hydrolase 4-like [Benincasa hispida][more]
XP_016902619.17.53e-17290.35PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo][more]
KAA0045542.13.85e-15389.08epoxide hydrolase 4-like [Cucumis melo var. makuwa] >TYK28850.1 epoxide hydrolas... [more]
XP_022947943.17.81e-15169.46epoxide hydrolase 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LLY65.23e-22398.68AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247... [more]
A0A1S4E3103.64e-17290.35uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=... [more]
A0A5A7TQB41.87e-15389.08Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1G8063.78e-15169.46epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1[more]
A0A7N2R8U79.28e-11453.00AB hydrolase-1 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G18360.13.0e-8046.77alpha/beta-Hydrolases superfamily protein [more]
AT4G36610.13.3e-7946.75alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.16.7e-4032.64alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.19.1e-3730.30alpha/beta-Hydrolases superfamily protein [more]
AT4G33180.14.5e-3628.97alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 51..165
e-value: 3.6E-14
score: 53.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..237
e-value: 7.2E-30
score: 106.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 43..228
NoneNo IPR availablePANTHERPTHR43139:SF22EPOXIDE HYDROLASE 4-LIKEcoord: 2..238
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 2..238

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4075.T2Cucsat.G4075.T2mRNA
Cucsat.G4075.T1Cucsat.G4075.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity