Cucsat.G3013.T8 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G3013.T8
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	E3 ubiquitin ligase BIG BROTHER isoform X1
Location	ctg1041: 6523021 .. 6526933 (-)
RNA-Seq Expression	Cucsat.G3013.T8
Synteny	Cucsat.G3013.T8

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATTTATATACTAAATTCTACAGTATAATTCACGTCAATATGTATATATATATATCTTTATCTTTGTTTTTCTTTTCTGAATTTTATTTTATTTTTCTTGAATAGTGTTTCTGACATAAAAACATGTTTGGCCAGCAAGAAAAATAAAATAAGATAAAATGAGGCCATGAACATAGAGTGGACCACCCTTTTATTTATTTATTTTTTCGAGAAAAATACAATCTTTTTCTTCTTTCTCTTTAGTTTATCGAAATAAATACAAGTAAAAAAAGAGAGAAACTTGTAAAATACCCATTTGCCATTTGAAGAAGATTTCAAAGCCCATTCCAAGCAGCTTAAAAACCTTCATTCATTTTGCAAAGAGATGGAAGAAAATGGATTATAGCCAGAAAACAAATTGAAGAACAAAGAAGGAAAGGAAGGGTACACAAAACCATATAACCCCATTTCCTTTCATTTTCATCTCTCTCTGCTAAGACTCTGCCAGAAATCAAGAGTTTTGGAGAGAGAGAGAAACAGAAAAAAAAAAAAAAAAAGGAGTTTTAGAGAGAGAGTTTAAGGTCATTCTTCCATTTTTCAAGCTTCTCTTGTACCTCATTTCTCAAGCCTTTATTTTTTGTTTGTTTGTTTGTTTGATAAGTTTTAGAAGAAGAAGCCTTTTTTTTTTGTTCTGTTTTTATTGTGGATATTATGAAGATTCTAATGAATACTGGGATCTGTACCTATTTATTTTTTGCTCATTTGTCTGTTTTTATCTGATTCTTAGGATTCTTTAACATCTTTAATACTCATTTGTCTACATTTGTCTAATTCCTTCAGATTTTTCCTCTGTCAGAGTGTGAAGAGTTTCTTGGGGAAGTTTCTGTTTGGCTATCAAGTAAGAAAAATCATAAGATTTTTCTTTGTGTTTTTCGTTTTGGAGACAAAAGAAGTTAAATGTAAGTTTTTCTTTTGGTTTTGTGTAATGTTCATGTTTGTTTTAAAGTTTTTTTTGAATGTTTTTAGGGTCTGTTTCCTATTTCATTGGATAGAAAATTTCAAATCTTCAATTTCCTTTGCTTTCTCATCTTTTCCTCTAAGTTTTATTATCAACCAAACAGATTGTTGTGGATAAGTTCATTCTAAAGCTTTGACAATGTAGGTTTTAAGTTCATTTCATATTTCATTTTGTTTGATATGTTATTTTCCTTTTGGTCTTTTTTGCAAATGTTACTGTGATTGTTGCTTAAGATGAATGAGGATAGACAAATGGAATTGCATTACATGGACACCGGCTTTCCTTACACGAATACAGAGAGCTTCATGGATTTCTTTCAAGGGCTTCACCAACATCAAGTGCCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGTAACAGAGCGGGTCATGAAAAACTGATGCAATTTATTTGTCAATGCTCGAGCTCGAGTTTTGTCATTTGAGAGAATAATATTGGAAATGCACGGTGATTTGTTTCTCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACGTATTACGGCCATTCGGAGGAAAGTCACCATCTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGCGATGAATGTCGAAATCCCCGAACCATCCTATGCGCCATCCGTGGAGGAAGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTAAGTGTTGACAAATTTGATACCGAGTCTGTTTATTTCATACTTTTCAAAGCTAACAATGAGTTTGTCACTATCACATCGTCCTGTTGAATCGTTGGTACGTTTGGATCTTACCAGCATCTTGTTACCTTGTTTCCTGTTTTGCAGGTGATTTAAGCCATCGGGAGGAAACTAGTACGTCTCAAGTATGATGCTTTCTCCTTGATTTCGCAATATGCCTTATTTTTCTGTTATGACCATGATCTACTTAATGCTCCTTACGAACCGTGGCTTAGTAAAAAAATTATTTGGTATCCTTTCACGTAGTTCAGTAAATAATGAACTAGGGTTTCATCTTATTATCATCAAAGGTTGTAGAATGTTCATCAAATGTTATTACTCTTAAAGTCTTAGTTTATGTCAGAAAGTTTCTCTTTCTGATCAGTTTTGTGCCCAACGAAGTTCCATTGCTTTTGAACTATGGGAATAGGTTCCTTTTTTATTTGGTAGGGCTCATTGTATCAGTCTTCTTTTGTTCGTCTCTTCAGGTTACATGGCAAGATGAGATTGATCCTGACCACATGACTTATGAGGTATGATTTCAACTCTATCATTTACAAACCAGATTTGTTTAAGAACATGTTTTAAGAAATGTTCTGTAAATCTTCAATAGATTGCCCTATAGAAGTTGAGGCTGTTCTTCACTTGAAACCAATGTTTTATTGAGCGCATGATTATTTTGGTTTAAAGAAAAAGATTTTTAGCTCAATTATCAACTAGCCGCTAGTTTCTTATGGCCATATGAACTATGGTTGGTTGTCAAATAAATGGGATCCACAGAAGTTTATTGTATAAAACAGAAGAGTATAAAAGGACTGCCTTTAGCCATGACTGTGCAGATGATCTTATATGGTTTTACAGGAACTGTTGGACTTGGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCACGATGCAAGTTAACGAGCTTTTTCTCAAGAAAAAAGCTCGATGAGAGGTCAGTTTTTGACAAATGTTACATTTCAGTTATCTTGCACCATCTTTGTTAATCTGCTTTACTTGGTGGGTATATGAGAAGACTCCTGTCAGAATAACTTTGAATCATCTGTCAATAATCGGTCTGGTGTCAATGTAGATGACAGTTTCTATAAACCGAGATGTTTAATCGGTTTGGTGTCAATGTGTCTACACTGAAGTCATATGGATCCATTTTTCTGTCATAGTACACGTTAATACATTCACTTTGGTTTCTTTAGTTTTTAGTGTAGCAATGTTGAACTCGAACCTCTAACCAATGTTACGAATAAGATAGTCATATGAGACACAAGAAGATGTTTACATCTTGGTACTTTCTCGATACAGTATTCATTACTGGAATATTTGATTGTACTTGTTTTGTTTCAACTGCAGATGTGTGATTTGCCAAATGAGGTACAAGCGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTCTACCACAACAAATGCATAACCAAATGGCTCACCATCAACAAAGTCAGTAATTTTCTTGGCATTCTCATATCATACTTCCACCATTTCTGTTCTAACTTGATGTTCTACCTCGTCACTCACCATCGTTTTTATCAGATATGTCCAATCTGCAACATCGAGGTGTTCGGAGATGGAAGCACGAATGCATCGGCCAGTGATAACTGACAGTAGATTTTGGAAGCTCTAACATTTTGTTCTTGTTCATCTTTTCCCATTTTTGCCTTCTCACTTTCATTTCTCTCCACATTTTGTAAATCTCTACTTAACTAAGCGAATCAAGTACACTCTAATCCTTACTCTCTCCCTCCCCACGCTTGCCTTATGATCAACAGAAAGAAAGAAAATGTAACCCCTCTTCTTAACTTGTTAACTGCTTAGCCTTAAGATTGAAGTCTAGTTATTTTCTTAGATTAAGATTAGATTATTTTGTAGTCATTTTACGGACTGGGGGTCGCCTCGAACTGCTTCAAATTAGGATGTTGTGGAGTTGGGGTGAGTTAGATTAGGTTAATATAGACGTTTTGGATCTAACCCAATTGTTCGAGTTACATATTTGTTCAATCTAAGTGACTCTTGTTAGTGAAAGACCATGAATTCATTTGATCATTTATTTCAAGTTTTGTAAAAGAAATAGTGAGAATGATATACT

Coding sequence (CDS)

ATGAATGAGGATAGACAAATGGAATTGCATTACATGGACACCGGCTTTCCTTACACGAATACAGAGAGCTTCATGGATTTCTTTCAAGGGCTTCACCAACATCAAGTGCCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACGTATTACGGCCATTCGGAGGAAAGTCACCATCTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGCGATGAATGTCGAAATCCCCGAACCATCCTATGCGCCATCCGTGGAGGAAGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTGATTTAAGCCATCGGGAGGAAACTAGTACGTCTCAAGTTACATGGCAAGATGAGATTGATCCTGACCACATGACTTATGAGGAACTGTTGGACTTGGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCACGATGCAAGTTAACGAGCTTTTTCTCAAGAAAAAAGCTCGATGAGAGATGTGTGATTTGCCAAATGAGGTACAAGCGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTCTACCACAACAAATGCATAACCAAATGGCTCACCATCAACAAAGTCAGTAATTTTCTTGGCATTCTCATATCATACTTCCACCATTTCTGTTCTAACTTGATGTTCTACCTCGTCACTCACCATCGTTTTTATCAGATATGTCCAATCTGCAACATCGAGGTGTTCGGAGATGGAAGCACGAATGCATCGGCCAGTGATAACTGA

Protein sequence

MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGILISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN

Homology

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.6e-58
Identity = 124/280 (44.29%), Postives = 166/280 (59.29%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFL 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INKV    
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV---- 240

Query: 241 GILISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGS 278
                                      CP+CN EVFG+ S
Sbjct: 241 ---------------------------CPVCNSEVFGEPS 246

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.4e-20
Identity = 78/226 (34.51%), Postives = 111/226 (49.12%), Query Frame = 0


Query: 45  LLDQGNAYW--SMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS----- 104
           L +Q  AY   +MN +   +G  + G+  Y + E+    P + D  + E EY T      
Sbjct: 104 LQEQERAYMMLTMNSEISDYGSWETGS--YVYDEDEFDDPENEDEDDDEDEYETDDDPQE 163

Query: 105 ---AMNVEIPEPSYAPSVEEDVVD-----------AHSIPEECDLSHR------------ 164
               +NV   E         D+ +           A    EE D++ R            
Sbjct: 164 DGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVV 223

Query: 165 ----EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSF 224
               +E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R K    
Sbjct: 224 EDLEDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD- 283

Query: 225 FSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKV 234
            ++   +E CVIC++ Y+  +  I LPCKH YH++CI  WL INKV
Sbjct: 284 -NQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKV 324

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-09
Identity = 34/89 (38.20%), Postives = 47/89 (52.81%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQMRY 204
           +D D+M+YEELL LGE +G  S GLSEE I  +       +S     +  E C +CQ  Y
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 205 KRGDKQIKLPCKHFYHNKCITKWLTINKV 234
             GD    L C H +H  C+ +WL +  +
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNL 655

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-09
Identity = 36/87 (41.38%), Postives = 51/87 (58.62%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDER-CVICQMR 204
           +D D+M+YEELL L E +G  S GLSEE+++ L   R K +S+     ++E  C ICQ  
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQR-KFSSWRLEASVEEEPCCICQEE 627

Query: 205 YKRGDKQIKLPCKHFYHNKCITKWLTI 231
           Y  GD    L C H +H  C+ +WL +
Sbjct: 628 YVDGDDLGTLDCGHDFHVGCVRQWLVV 653

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-09
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLD---ERCVICQ 204
           +D D+M+YEELL LGE +G  S GLSEE I          +S  S  +L    E C ICQ
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQ 660

Query: 205 MRYKRGDKQIKLPCKHFYHNKCITKWLTINKV 234
             Y  GD    L C H +H  CI +W+ I  +
Sbjct: 661 EEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNL 692

[Close]

BLAST of Cucsat.G3013.T8 vs. NCBI nr
Match: XP_011659453.1 (E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011659454.1 E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >KAE8646405.1 hypothetical protein Csa_015770 [Cucumis sativus])

HSP 1 Score: 516 bits (1330), Expect = 2.59e-184
Identity = 254/285 (89.12%), Postives = 254/285 (89.12%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------------ICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. NCBI nr
Match: XP_008461282.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_008461283.1 PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo])

HSP 1 Score: 515 bits (1326), Expect = 1.06e-183
Identity = 253/285 (88.77%), Postives = 254/285 (89.12%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------------ICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. NCBI nr
Match: TYK10442.1 (E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 513 bits (1320), Expect = 8.67e-183
Identity = 252/285 (88.42%), Postives = 253/285 (88.77%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------------ICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. NCBI nr
Match: XP_008461284.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo])

HSP 1 Score: 503 bits (1294), Expect = 6.39e-179
Identity = 247/279 (88.53%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGILISYFHH 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK              
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------------- 240

Query: 247 FCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                            ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------ICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Cucsat.G3013.T8 vs. NCBI nr
Match: XP_038899262.1 (E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899264.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida])

HSP 1 Score: 489 bits (1259), Expect = 1.85e-173
Identity = 240/285 (84.21%), Postives = 247/285 (86.67%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSH+EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           R KLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGS NAS +DN
Sbjct: 241 -----------------------ICPICNIEVFGDGSMNASGNDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL
Match: A0A1S3CED1 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 515 bits (1326), Expect = 5.11e-184
Identity = 253/285 (88.77%), Postives = 254/285 (89.12%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------------ICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL
Match: A0A5D3CGQ8 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00890 PE=4 SV=1)

HSP 1 Score: 513 bits (1320), Expect = 4.20e-183
Identity = 252/285 (88.42%), Postives = 253/285 (88.77%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGIL 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK        
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------- 240

Query: 241 ISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                  ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------------ICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL
Match: A0A0A0K8C4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 PE=4 SV=1)

HSP 1 Score: 504 bits (1298), Expect = 7.60e-180
Identity = 248/279 (88.89%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGILISYFHH 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK              
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------------- 240

Query: 247 FCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                            ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------ICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL
Match: A0A1S3CES7 (E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 3.10e-179
Identity = 247/279 (88.53%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLGILISYFHH 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK              
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK-------------- 240

Query: 247 FCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                            ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -----------------ICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL
Match: A0A5A7UTY6 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001030 PE=4 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 2.46e-171
Identity = 242/287 (84.32%), Postives = 245/287 (85.37%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECD--LSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP 180
           SIPEEC   +      S+ QV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP
Sbjct: 121 SIPEECKWLIVSVFFCSSLQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP 180

Query: 181 TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFLG 240
           TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK      
Sbjct: 181 TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINK------ 240

Query: 241 ILISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGSTNASASDN 285
                                    ICPICNIEVFGDGSTNASASDN
Sbjct: 241 -------------------------ICPICNIEVFGDGSTNASASDN 256

[Close]

BLAST of Cucsat.G3013.T8 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 225.7 bits (574), Expect = 4.7e-59
Identity = 124/280 (44.29%), Postives = 166/280 (59.29%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFL 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INKV    
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV---- 240

Query: 241 GILISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGS 278
                                      CP+CN EVFG+ S
Sbjct: 241 ---------------------------CPVCNSEVFGEPS 246

[Close]

BLAST of Cucsat.G3013.T8 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 225.7 bits (574), Expect = 4.7e-59
Identity = 124/280 (44.29%), Postives = 166/280 (59.29%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKVSNFL 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INKV    
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV---- 240

Query: 241 GILISYFHHFCSNLMFYLVTHHRFYQICPICNIEVFGDGS 278
                                      CP+CN EVFG+ S
Sbjct: 241 ---------------------------CPVCNSEVFGEPS 246

[Close]

BLAST of Cucsat.G3013.T8 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.9e-21
Identity = 78/226 (34.51%), Postives = 111/226 (49.12%), Query Frame = 0


Query: 45  LLDQGNAYW--SMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS----- 104
           L +Q  AY   +MN +   +G  + G+  Y + E+    P + D  + E EY T      
Sbjct: 104 LQEQERAYMMLTMNSEISDYGSWETGS--YVYDEDEFDDPENEDEDDDEDEYETDDDPQE 163

Query: 105 ---AMNVEIPEPSYAPSVEEDVVD-----------AHSIPEECDLSHR------------ 164
               +NV   E         D+ +           A    EE D++ R            
Sbjct: 164 DGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVV 223

Query: 165 ----EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSF 224
               +E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R K    
Sbjct: 224 EDLEDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD- 283

Query: 225 FSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKV 234
            ++   +E CVIC++ Y+  +  I LPCKH YH++CI  WL INKV
Sbjct: 284 -NQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKV 324

[Close]

BLAST of Cucsat.G3013.T8 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 2.6e-17
Identity = 59/171 (34.50%), Postives = 86/171 (50.29%), Query Frame = 0


Query: 74  HSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV---DAHSIPEECDLSH 133
           H E +++    +        +P       IP+   APS    +    D  +       +H
Sbjct: 101 HDELANNTEDDIQETHHHQYHPMQVNRSYIPQRD-APSSSRAIFHYDDGENFSGRYTHTH 160

Query: 134 REETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTAR--CKLTSFF 193
              + +   T  +  DP++MTYEEL +LG+SVG   +GLS+E+IS L T +   K  S++
Sbjct: 161 SPSSISHNPTDDENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWY 220

Query: 194 ---SRKKL---DERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKV 234
               +KK    D +C IC M Y +GDK   LPCKH YH  CI++WL  NKV
Sbjct: 221 CLHMKKKFVADDSQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKV 270

[Close]

BLAST of Cucsat.G3013.T8 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-16
Identity = 49/124 (39.52%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 112 VEEDVVDAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSE 171
           V+ED ++     +E + +  EE    +   +DEIDPD ++YEEL+ LG+ +G E+RGL+ 
Sbjct: 81  VDEDEINEFLEDQESNSNLEEEDDFLEE--EDEIDPDQLSYEELIALGDFIGVENRGLTP 140

Query: 172 EQISLLPTARCKLTSFFSRKKLD-ERCVICQMRYKRGDKQIKL-PCKHFYHNKCITKWLT 231
            +IS    A    T  FS  K + +RCV+CQM ++  +  + L PC H YH++CITKWL 
Sbjct: 141 IEISTCLNAS---TYVFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLE 199

Query: 232 INKV 234
             K+
Sbjct: 201 TKKI 199

The following BLAST results are available for this feature:

BLAST of Cucsat.G3013.T8 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	6.6e-58	44.29	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	5.4e-20	34.51	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	1.1e-09	38.20	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q7XTV7	1.5e-09	41.38	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9ZQF9	2.5e-09	41.30	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]

BLAST of Cucsat.G3013.T8 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659453.1	2.59e-184	89.12	E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011659454.1 E3 ubi...	[more]
XP_008461282.1	1.06e-183	88.77	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_0084612...	[more]
TYK10442.1	8.67e-183	88.42	E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008461284.1	6.39e-179	88.53	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo]	[more]
XP_038899262.1	1.85e-173	84.21	E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 u...	[more]

BLAST of Cucsat.G3013.T8 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CED1	5.11e-184	88.77	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A5D3CGQ8	4.20e-183	88.42	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0K8C4	7.60e-180	88.89	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 P...	[more]
A0A1S3CES7	3.10e-179	88.53	E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A5A7UTY6	2.46e-171	84.32	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of Cucsat.G3013.T8 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	4.7e-59	44.29	RING/U-box superfamily protein	[more]
AT3G63530.2	4.7e-59	44.29	RING/U-box superfamily protein	[more]
AT3G19910.1	3.9e-21	34.51	RING/U-box superfamily protein	[more]
AT5G52140.1	2.6e-17	34.50	RING/U-box superfamily protein	[more]
AT3G47180.1	1.7e-16	39.52	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 159..235 e-value: 2.8E-13 score: 51.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 196..231 e-value: 8.9E-9 score: 35.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 197..269 score: 9.1551
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..278
None	No IPR available	PANTHER	PTHR46400:SF3	E3 UBIQUITIN-PROTEIN LIGASE BIG BROTHER	coord: 1..278
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 196..233

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G3013	Cucsat.G3013	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G3013.T8.E1	Cucsat.G3013.T8.E1	exon
Cucsat.G3013.T8.E2	Cucsat.G3013.T8.E2	exon
Cucsat.G3013.T8.E3	Cucsat.G3013.T8.E3	exon
Cucsat.G3013.T8.E4	Cucsat.G3013.T8.E4	exon
Cucsat.G3013.T8.E5	Cucsat.G3013.T8.E5	exon
Cucsat.G3013.T8.E6	Cucsat.G3013.T8.E6	exon
Cucsat.G3013.T8.E7	Cucsat.G3013.T8.E7	exon
Cucsat.G3013.T8.E8	Cucsat.G3013.T8.E8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G3013.T8.C6	Cucsat.G3013.T8.C6	CDS
Cucsat.G3013.T8.C5	Cucsat.G3013.T8.C5	CDS
Cucsat.G3013.T8.C4	Cucsat.G3013.T8.C4	CDS
Cucsat.G3013.T8.C3	Cucsat.G3013.T8.C3	CDS
Cucsat.G3013.T8.C2	Cucsat.G3013.T8.C2	CDS
Cucsat.G3013.T8.C1	Cucsat.G3013.T8.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G3013.T8	Cucsat.G3013.T8-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0048437	floral organ development
biological_process	GO:0008285	negative regulation of cell population proliferation
biological_process	GO:1900057	positive regulation of leaf senescence
biological_process	GO:0051865	protein autoubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding