Cucsat.G241.T3 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G241.T3 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-39
Identity = 109/316 (34.49%), Postives = 168/316 (53.16%), Query Frame = 0

Query: 29  PPLSPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPST 88
           P L   +LL       ++ LP  S P +    +  +    +L+P++V  G I+   +PS 
Sbjct: 73  PALRSCQLLCKAEANISSNLPE-SIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSM 132

Query: 89  FSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVL 148
            +W        +++ LG +ML+MGLTL  +D F   M+ P ++  G +AQY I P     
Sbjct: 133 VTWL---ETDLFTVGLGFLMLSMGLTLTFED-FRRCMRNPWTVGVGFLAQYLIKPMLGFA 192

Query: 149 IGKLLGLSQSLLFGLVLLGCCPGGSASNVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLT 208
           I   L LS  L  GL+L+ CCPGG ASNV T I++G+V LS++MT C+T+GA++ TP LT
Sbjct: 193 IAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIVMTPLLT 252

Query: 209 KFLVGAYIPVDAAQLSLSTLQVVVAPILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLA 268
           K L G  +PVDAA L++ST QVV+ P ++G    + FP   + ++   PL+ VL ++LL 
Sbjct: 253 KLLAGQLVPVDAAGLAISTFQVVLLPTIVGVLAHEYFPKFTERIISITPLIGVLLTTLLC 312

Query: 269 SSVFSENVIRIKSSMVSATLASDASLWTVLKSILSGELGVVILSVFCLHFAGFFVGYIAA 328
           +S   +                       +  +L  + G +I+ V  LH A F +GY  +
Sbjct: 313 ASPIGQ-----------------------VSEVLKAQGGQLIIPVALLHVAAFALGYWLS 360

Query: 329 AICGFRERERRTISMQ 345
            +  F E   RTIS++
Sbjct: 373 KVSSFGESTSRTISIE 360

BLAST of Cucsat.G241.T3 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 112/307 (36.48%), Postives = 168/307 (54.72%), Query Frame = 0

Query: 45  NNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSL 104
           +N+LP+         ++      ++ +P++V+ G ++  + PSTF+W     P+   + L
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGL 134

Query: 105 GLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLV 164
            + ML MG+TL + DL    +  P  +  G + QY++MP SA  + KLL L      GL+
Sbjct: 135 TITMLGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLI 194

Query: 165 LLGCCPGGSASNVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLS 224
           L+GCCPGG+ASN+VT IA+G+V LS++MT  +T+ AVI TP LT  L   YI VDA  L 
Sbjct: 195 LVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLL 254

Query: 225 LSTLQVVVAPILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMV 284
           +STLQVV+ P+L G+ L + F  LVK V P  P +AV T ++L      +N   I     
Sbjct: 255 MSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAI----- 314

Query: 285 SATLASDASLWTVLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQ 344
                           ++SG+   V+L+   LH +GF  GY+ + I G      RTIS++
Sbjct: 315 ----------------LMSGK--QVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIE 354

Query: 345 V--QNDI 350
           V  QN +
Sbjct: 375 VGMQNSV 354

BLAST of Cucsat.G241.T3 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.4e-38
Identity = 113/324 (34.88%), Postives = 171/324 (52.78%), Query Frame = 0

Query: 27  PIPPL------SPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGI 86
           PI P+      S  R++   +   + +LP  S P + +  +  +    +L+PL+V  G +
Sbjct: 56  PISPVFALEATSSRRVVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGTL 115

Query: 87  VACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYT 146
           V   +PS  +W        ++L LG +ML+MGLTL  +D F   ++ P ++  G +AQY 
Sbjct: 116 VGIFKPSLVTWL---ETDLFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYM 175

Query: 147 IMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTLIAQGDVPLSIVMTVCTTLGA 206
           I P    LI   L LS  L  GL+L+ CCPGG ASNV T I++G+V LS++MT C+T+GA
Sbjct: 176 IKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGA 235

Query: 207 VIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSCLQKAFPSLVKLVLPFAPLVA 266
           +I TP LTK L G  +PVDAA L+LST QVV+ P ++G    + FP     ++   PL+ 
Sbjct: 236 IIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIG 295

Query: 267 VLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKSILSGELGVVILSVFCLHFAG 326
           V+ ++LL +S   +                       +  +L  +   +IL V  LH A 
Sbjct: 296 VILTTLLCASPIGQ-----------------------VADVLKTQGAQLILPVALLHAAA 350

Query: 327 FFVGYIAAAICGFRERERRTISMQ 345
           F +GY  +    F E   RTIS++
Sbjct: 356 FAIGYWISKF-SFGESTSRTISIE 350

BLAST of Cucsat.G241.T3 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 3.0e-36
Identity = 104/281 (37.01%), Postives = 150/281 (53.38%), Query Frame = 0

Query: 71  YPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLS 130
           +P++V +   VA   P  F W     P +  + +   ML MG+TL + DL    +  P  
Sbjct: 106 FPVWVASACAVALWRPPAFLWV---SPMAQIVGISFTMLGMGMTLTLDDLKTALLM-PKE 165

Query: 131 ILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTLIAQGDVPLSI 190
           +  G + QY++MP S  LI KLL L      GL+L+ CCPGG+ASN+VT +A+G+V LS+
Sbjct: 166 LASGFLLQYSVMPLSGFLISKLLNLPSYYAAGLILVSCCPGGTASNIVTYLARGNVALSV 225

Query: 191 VMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSCLQKAFPSLVK 250
           +MT  +T  A   TP LT  L G Y+ VD   L +ST QVV+AP+LLG+ L +    LV+
Sbjct: 226 LMTAASTFAAAFLTPLLTSKLAGQYVAVDPMGLFVSTSQVVLAPVLLGALLNQYCNGLVQ 285

Query: 251 LVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKSILSGELGVVI 310
           LV P  P +AV T ++L  +  ++N   I SS +                        V+
Sbjct: 286 LVSPLMPFIAVATVAVLCGNAIAQNASAILSSGLQ-----------------------VV 345

Query: 311 LSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV--QNDI 350
           +SV  LH +GFF GY+ +   G      RTIS++V  QN +
Sbjct: 346 MSVCWLHASGFFFGYVLSRTIGIDISSSRTISIEVGMQNSV 359

BLAST of Cucsat.G241.T3 vs. ExPASy Swiss-Prot
Match: Q6K739 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.2e-30
Identity = 98/295 (33.22%), Postives = 148/295 (50.17%), Query Frame = 0

Query: 51  PSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLA 110
           P   P      D     ++L PL V A  + A   P+TFSW        Y+ +LG IML+
Sbjct: 88  PVSSPSSAAAGDPSQALSALLPLVVAATAVAALGNPATFSWV---SKEYYAPALGGIMLS 147

Query: 111 MGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCP 170
           +G+ L I D F L  +RP+ +  G +AQY + P   VLI +  G+  +   G VL  C  
Sbjct: 148 IGIKLSIDD-FALAFKRPVPLTIGYMAQYIVKPLMGVLIARAFGMPSAFFAGFVLTCCVS 207

Query: 171 GGSASNVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQV 230
           G   S+  + +++GDV LSI++T C+T+ +V+ TP LT  L+G+ +PVD   ++ S LQV
Sbjct: 208 GAQLSSYASFLSKGDVALSILLTSCSTISSVVVTPVLTGLLIGSVVPVDGIAMAKSILQV 267

Query: 231 VVAPILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLAS 290
           V+ P+ LG  L     ++V ++ P  P VA+L +SL   S  + N               
Sbjct: 268 VLVPVTLGLLLNTYAKAVVNVIQPVMPFVAMLCTSLCIGSPLAIN--------------- 327

Query: 291 DASLWTVLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERER--RTISM 344
                     ILS E  +++L +   H A F VGY  + +   R+ E   RTIS+
Sbjct: 328 -------RSKILSSEGFLLLLPIVTFHIAAFIVGYWISKLPMLRQEEPVCRTISV 356

BLAST of Cucsat.G241.T3 vs. NCBI nr
Match: XP_004133714.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis sativus] >KGN56248.1 hypothetical protein Csa_010831 [Cucumis sativus])

HSP 1 Score: 634 bits (1635), Expect = 3.50e-227
Identity = 343/345 (99.42%), Postives = 343/345 (99.42%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPR LTVRSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRSLTVRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180
           FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL
Sbjct: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180

Query: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240
           IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC
Sbjct: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240

Query: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300
           LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS
Sbjct: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300

Query: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV
Sbjct: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345

BLAST of Cucsat.G241.T3 vs. NCBI nr
Match: XP_008452255.1 (PREDICTED: probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis melo] >KAA0060620.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa] >TYK02252.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 613 bits (1581), Expect = 5.65e-219
Identity = 329/345 (95.36%), Postives = 338/345 (97.97%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNVVTL
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNVVTL 180

Query: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240
           IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC
Sbjct: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240

Query: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300
           LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVL+S
Sbjct: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLQS 300

Query: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ILSGELGVVILSVFCLHFAGFFVGYIAAAICGF+ERERRTISMQV
Sbjct: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFQERERRTISMQV 345

BLAST of Cucsat.G241.T3 vs. NCBI nr
Match: XP_038903139.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 553 bits (1426), Expect = 2.66e-195
Identity = 308/349 (88.25%), Postives = 319/349 (91.40%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRP-NLRLHRPPIPPLSPPRL---LTVRSVQQNNELPSPSPPPK 60
           M PISLQLTPFISPL+HR  NL L RPPIP LSPP+L   L VRSV +NNE PSPS P K
Sbjct: 1   MAPISLQLTPFISPLLHRRRNLSLRRPPIPSLSPPKLPRSLPVRSVHRNNEHPSPSHPRK 60

Query: 61  PTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLE 120
            TGLDDFLSTAASLYPLYVTAGGIVACL+PSTFSWFVQRGP+SYS SLGLIMLAMGLTLE
Sbjct: 61  STGLDDFLSTAASLYPLYVTAGGIVACLKPSTFSWFVQRGPASYSFSLGLIMLAMGLTLE 120

Query: 121 IKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASN 180
           +KDLFNLFMQRPLSILFGCVAQYTIMPASA LIGK  GLS SLL GL+LLGCCPGG+ASN
Sbjct: 121 LKDLFNLFMQRPLSILFGCVAQYTIMPASAALIGKFFGLSPSLLVGLILLGCCPGGTASN 180

Query: 181 VVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPIL 240
           VVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTK L GAYIPVDAAQLSLSTLQVVVAPIL
Sbjct: 181 VVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKILAGAYIPVDAAQLSLSTLQVVVAPIL 240

Query: 241 LGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWT 300
           LGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLA SVFSENV R KSSMV ATLASDASLWT
Sbjct: 241 LGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLACSVFSENVARFKSSMVIATLASDASLWT 300

Query: 301 VLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           VL+SILSGELGVVILSVFCLHFAGFFVGYI AAI GFRERERR IS++V
Sbjct: 301 VLQSILSGELGVVILSVFCLHFAGFFVGYITAAIGGFRERERRAISIEV 349

BLAST of Cucsat.G241.T3 vs. NCBI nr
Match: XP_022985149.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 527 bits (1357), Expect = 8.63e-185
Identity = 286/348 (82.18%), Postives = 311/348 (89.37%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPPKP 60
           M  ISLQ TPFISPL HR NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  TGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEI 120
           +GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTLE+
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 KDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 180
           KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS  L  GL+LL CCPGG+ASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILL 240
           VTLIAQGDVPLSIVMTVCTTLGAVI TPFLTK L GAYIPVDAA+LSLSTLQVVVAPILL
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPILL 240

Query: 241 GSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTV 300
           GS LQKAFP LVKLV+PFAPLVAVLTSSLLA SVFSENV+R KSSMV+A+LASDAS W +
Sbjct: 241 GSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPWMI 300

Query: 301 LKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ++SILSGELG+V+LSVFCLHFAGFFVGY+AA+I GFRERERR IS++V
Sbjct: 301 IRSILSGELGMVVLSVFCLHFAGFFVGYVAASIGGFRERERRAISIEV 348

BLAST of Cucsat.G241.T3 vs. NCBI nr
Match: XP_022929395.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 525 bits (1351), Expect = 7.57e-184
Identity = 290/350 (82.86%), Postives = 311/350 (88.86%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIH--RPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPP 60
           M  ISLQ TPFISPL H  R NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP 
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 120
           KP+GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSAS 180
           E+KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS SL  GL+LL CCPGG+AS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

Query: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPI 240
           NVVTLIAQGDVPLSIVMTVCTTLGAVI TPFLTK L GAYIPVDAA+LSLSTLQVVVAPI
Sbjct: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPI 240

Query: 241 LLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLW 300
           LLGS LQKAFP LVKLV+PFAPLVAVLTSSLLA SVFSENV+R KSSMV+A+LASDAS W
Sbjct: 241 LLGSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPW 300

Query: 301 TVLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
            V++SILSGELG VILSVFCLHFAGFFVGYIAA+I GFRERERR IS++V
Sbjct: 301 MVIRSILSGELGTVILSVFCLHFAGFFVGYIAASIGGFRERERRAISIEV 350

BLAST of Cucsat.G241.T3 vs. ExPASy TrEMBL
Match: A0A0A0L3A1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G106000 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 1.70e-227
Identity = 343/345 (99.42%), Postives = 343/345 (99.42%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPR LTVRSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRSLTVRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180
           FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL
Sbjct: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180

Query: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240
           IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC
Sbjct: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240

Query: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300
           LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS
Sbjct: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300

Query: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV
Sbjct: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345

BLAST of Cucsat.G241.T3 vs. ExPASy TrEMBL
Match: A0A5D3BTX1 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00520 PE=3 SV=1)

HSP 1 Score: 613 bits (1581), Expect = 2.74e-219
Identity = 329/345 (95.36%), Postives = 338/345 (97.97%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNVVTL
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNVVTL 180

Query: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240
           IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC
Sbjct: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240

Query: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300
           LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVL+S
Sbjct: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLQS 300

Query: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ILSGELGVVILSVFCLHFAGFFVGYIAAAICGF+ERERRTISMQV
Sbjct: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFQERERRTISMQV 345

BLAST of Cucsat.G241.T3 vs. ExPASy TrEMBL
Match: A0A1S3BSU0 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493327 PE=3 SV=1)

HSP 1 Score: 613 bits (1581), Expect = 2.74e-219
Identity = 329/345 (95.36%), Postives = 338/345 (97.97%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 180
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNVVTL
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNVVTL 180

Query: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240
           IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC
Sbjct: 181 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 240

Query: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 300
           LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVL+S
Sbjct: 241 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLQS 300

Query: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ILSGELGVVILSVFCLHFAGFFVGYIAAAICGF+ERERRTISMQV
Sbjct: 301 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFQERERRTISMQV 345

BLAST of Cucsat.G241.T3 vs. ExPASy TrEMBL
Match: A0A6J1JCH6 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483238 PE=3 SV=1)

HSP 1 Score: 527 bits (1357), Expect = 4.18e-185
Identity = 286/348 (82.18%), Postives = 311/348 (89.37%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPPKP 60
           M  ISLQ TPFISPL HR NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  TGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEI 120
           +GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTLE+
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 KDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 180
           KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS  L  GL+LL CCPGG+ASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILL 240
           VTLIAQGDVPLSIVMTVCTTLGAVI TPFLTK L GAYIPVDAA+LSLSTLQVVVAPILL
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPILL 240

Query: 241 GSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTV 300
           GS LQKAFP LVKLV+PFAPLVAVLTSSLLA SVFSENV+R KSSMV+A+LASDAS W +
Sbjct: 241 GSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPWMI 300

Query: 301 LKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
           ++SILSGELG+V+LSVFCLHFAGFFVGY+AA+I GFRERERR IS++V
Sbjct: 301 IRSILSGELGMVVLSVFCLHFAGFFVGYVAASIGGFRERERRAISIEV 348

BLAST of Cucsat.G241.T3 vs. ExPASy TrEMBL
Match: A0A6J1EUA3 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435979 PE=3 SV=1)

HSP 1 Score: 525 bits (1351), Expect = 3.67e-184
Identity = 290/350 (82.86%), Postives = 311/350 (88.86%), Query Frame = 0

Query: 1   MPPISLQLTPFISPLIH--RPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPP 60
           M  ISLQ TPFISPL H  R NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP 
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 120
           KP+GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSAS 180
           E+KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS SL  GL+LL CCPGG+AS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

Query: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPI 240
           NVVTLIAQGDVPLSIVMTVCTTLGAVI TPFLTK L GAYIPVDAA+LSLSTLQVVVAPI
Sbjct: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPI 240

Query: 241 LLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLW 300
           LLGS LQKAFP LVKLV+PFAPLVAVLTSSLLA SVFSENV+R KSSMV+A+LASDAS W
Sbjct: 241 LLGSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPW 300

Query: 301 TVLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQV 345
            V++SILSGELG VILSVFCLHFAGFFVGYIAA+I GFRERERR IS++V
Sbjct: 301 MVIRSILSGELGTVILSVFCLHFAGFFVGYIAASIGGFRERERRAISIEV 350

BLAST of Cucsat.G241.T3 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 161.8 bits (408), Expect = 1.0e-39
Identity = 112/307 (36.48%), Postives = 168/307 (54.72%), Query Frame = 0

Query: 45  NNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSL 104
           +N+LP+         ++      ++ +P++V+ G ++  + PSTF+W     P+   + L
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGL 134

Query: 105 GLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLV 164
            + ML MG+TL + DL    +  P  +  G + QY++MP SA  + KLL L      GL+
Sbjct: 135 TITMLGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLI 194

Query: 165 LLGCCPGGSASNVVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLS 224
           L+GCCPGG+ASN+VT IA+G+V LS++MT  +T+ AVI TP LT  L   YI VDA  L 
Sbjct: 195 LVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLL 254

Query: 225 LSTLQVVVAPILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMV 284
           +STLQVV+ P+L G+ L + F  LVK V P  P +AV T ++L      +N   I     
Sbjct: 255 MSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAI----- 314

Query: 285 SATLASDASLWTVLKSILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQ 344
                           ++SG+   V+L+   LH +GF  GY+ + I G      RTIS++
Sbjct: 315 ----------------LMSGK--QVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIE 354

Query: 345 V--QNDI 350
           V  QN +
Sbjct: 375 VGMQNSV 354

BLAST of Cucsat.G241.T3 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 159.1 bits (401), Expect = 6.7e-39
Identity = 113/324 (34.88%), Postives = 171/324 (52.78%), Query Frame = 0

Query: 27  PIPPL------SPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGI 86
           PI P+      S  R++   +   + +LP  S P + +  +  +    +L+PL+V  G +
Sbjct: 56  PISPVFALEATSSRRVVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGTL 115

Query: 87  VACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYT 146
           V   +PS  +W        ++L LG +ML+MGLTL  +D F   ++ P ++  G +AQY 
Sbjct: 116 VGIFKPSLVTWL---ETDLFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYM 175

Query: 147 IMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTLIAQGDVPLSIVMTVCTTLGA 206
           I P    LI   L LS  L  GL+L+ CCPGG ASNV T I++G+V LS++MT C+T+GA
Sbjct: 176 IKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGA 235

Query: 207 VIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSCLQKAFPSLVKLVLPFAPLVA 266
           +I TP LTK L G  +PVDAA L+LST QVV+ P ++G    + FP     ++   PL+ 
Sbjct: 236 IIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIG 295

Query: 267 VLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKSILSGELGVVILSVFCLHFAG 326
           V+ ++LL +S   +                       +  +L  +   +IL V  LH A 
Sbjct: 296 VILTTLLCASPIGQ-----------------------VADVLKTQGAQLILPVALLHAAA 350

Query: 327 FFVGYIAAAICGFRERERRTISMQ 345
           F +GY  +    F E   RTIS++
Sbjct: 356 FAIGYWISKF-SFGESTSRTISIE 350

BLAST of Cucsat.G241.T3 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 120.6 bits (301), Expect = 2.6e-27
Identity = 92/278 (33.09%), Postives = 140/278 (50.36%), Query Frame = 0

Query: 68  ASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQR 127
           ++L P  V    + A   P +F+W        Y+ +LG IML++G+ L + D F L  +R
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWV---SKDLYAPALGGIMLSIGIQLSVDD-FALAFKR 171

Query: 128 PLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTLIAQGDVP 187
           P+ +  G VAQY + P   VL+    G+ ++   G +L  C  G   S+  + +++ DV 
Sbjct: 172 PVPLSVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVA 231

Query: 188 LSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSCLQKAFPS 247
           +SI++T  TT+ +VIFTP L+  L+G+ +PVDA  +S S LQVV+ PI LG  L      
Sbjct: 232 MSILLTSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKP 291

Query: 248 LVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKSILSGELG 307
           +V L+ P  P VA++ +SL   S  S N                         ILS E  
Sbjct: 292 VVTLLQPVMPFVAMVCTSLCIGSPLSIN----------------------RSQILSAEGL 351

Query: 308 VVILSVFCLHFAGFFVGYIAAAICGFRERER--RTISM 344
            +I+ +   H   F +GY  + I G R+ E   RTIS+
Sbjct: 352 GLIVPIVTFHAVAFALGYWFSKIPGLRQEEEVSRTISL 363

BLAST of Cucsat.G241.T3 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 109.4 bits (272), Expect = 6.1e-24
Identity = 87/273 (31.87%), Postives = 132/273 (48.35%), Query Frame = 0

Query: 62  DFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLF 121
           D +  A S+ P  V A  I+A + P +F+WF  R    +  +LG +M A+G+    KD  
Sbjct: 92  DRMKKANSILPHVVLASTILALIYPPSFTWFTSR---YFVPALGFLMFAVGINSNEKDFL 151

Query: 122 NLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLF--------GLVLLGCCPGGS 181
             F +RP +IL G V QY + P    ++G + GL+   LF        G++L+ C  G  
Sbjct: 152 EAF-KRPKAILLGYVGQYLVKP----VLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQ 211

Query: 182 ASNVVTLIAQGDV-PLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVV 241
            SN  T +    + PLSIVMT  +T  AV+ TP L+  L+G  +PVD   +  S LQVV+
Sbjct: 212 LSNYATFLTDPALAPLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVI 271

Query: 242 APILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDA 301
           API  G  L K FP +   + PF P+++VL ++    +  + N+                
Sbjct: 272 APIAAGLLLNKLFPKVSNAIRPFLPILSVLDTACCVGAPLALNI---------------- 331

Query: 302 SLWTVLKSILSGELGVVILSVFCLHFAGFFVGY 326
                  S++S     ++L V   H + F  GY
Sbjct: 332 ------NSVMSPFGATILLLVTMFHLSAFLAGY 334

BLAST of Cucsat.G241.T3 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 92.0 bits (227), Expect = 1.0e-18
Identity = 81/275 (29.45%), Postives = 121/275 (44.00%), Query Frame = 0

Query: 56  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 115
           K + + + L  A S  P  +    I+A + P +F+WF    P  +   LG +M A+G+  
Sbjct: 87  KVSSIMETLKQAYSFIPHGILLSTILALVYPPSFTWF---KPRYFVPGLGFMMFAVGINS 146

Query: 116 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIG----KLLGLSQSLLFGLVLLGCCPG 175
             +D     ++RP +I  G + QY I P    + G     L  L  S+  G++L+ C  G
Sbjct: 147 NERDFLEA-LKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSG 206

Query: 176 GSASNVVTLIAQGDV-PLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQV 235
              SN  T +    +  LSIVMT  +T  AV+ TP L+  L+G  +PVD   +  S LQV
Sbjct: 207 AQLSNYTTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQV 266

Query: 236 VVAPILLGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLAS 295
           V+ PI  G  L + FP L   + PF P + V+  S    +  + N+              
Sbjct: 267 VITPIAAGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLALNI-------------- 326

Query: 296 DASLWTVLKSILSGELGVVILSVFCLHFAGFFVGY 326
                    SILS     ++  V   H   F  GY
Sbjct: 327 --------DSILSPFGATILFLVITFHLLAFVAGY 335