Cucsat.G241.T16 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G241.T16
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Bile acid:sodium symporter
Location	ctg1: 5379122 .. 5382243 (-)
RNA-Seq Expression	Cucsat.G241.T16
Synteny	Cucsat.G241.T16

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAAAAATGTTGTTAGAAAAATTATCAAATTCCAACCTCTTGCTTTGGACCAATATTTCTACCAATTTGTTGATAGCGATCTCTTGCTTCCTAATAATCTCCCAACACAATCTTGTCGTTTCACAAAATACAACCCGTGTTTGTATGCATCACTTCACCCAATTTCTATCATCTCCTTCAATTCTCCTTCTTTTCTTCACTAATCGGAGCCACAGAATTCTGCACAATGCCTCCGATTTCTCTGCAACTCACCCCCTTCATTTCTCCACTAATCCACCGCCCTAATCTCCGCCTTCACCGACCCCCAATTCCCCCTCTCTCGCCGCCCAGATTACTCACCGTCCGATCGGTTCAACAAAATAACGAACTCCCATCTCCCTCGCCACCGCCAAAACCCACTGGTTTGGACGATTTTCTTTCCACGGCGGCTAGTCTCTACCCTCTCTATGTGACGGCAGGCGGGATTGTTGCTTGCCTCGAACCCTCGACCTTCTCGTGGTTTGTGCAGAGAGGGCCGAGTTCCTATAGCTTATCTCTTGGTTTGATAATGTTGGCCATGGGGCTTACTCTAGAGATCAAGGATTTATTTAATTTGTTCATGCAAAGACCTCTTTCGGTGAGTTTTTGGAATCTGAATTTGTTTACATTGTGTGTATTTTGGTTATTGATTTGATTTGATGGGATGGAACCGGGGGTTTGTGGTAATGCTTGATGGTTTTGACTCTGTTGCTTTGTTTTAGATATTGTTTGGATGTGTAGCTCAGTACACGATTATGCCAGCTTCTGCAGTGCTGATCGGAAAACTTTTGGGACTTTCCCAGTCCCTTTTGTTCGGTTTGGTCTTGCTTGGATGTTGTCCTGGAGGATCTGCTTCCAATGTGGTAATAATGCCTATGATCTCTGTTTTCTTATTTGTTCTGTTTAATTTCTTATAACTTTTGTTTCTCTCCTAAAAACGGCAAAAAAAAAAAAAAAAAAGAGTAGAGAGTGAAGAGCCGCAACCTACTAGGAAATAAAATAATGTTCAAAAGATAATCCTGTATTTTAAAATTTAGAATTCAAAATCTCGATACAATACAAACATAAAAATGTTTTTTCAGAATATGTTCTTCTTGGATCACATAATTCAAGAACAGTGTTAAAAAAAATGTTGAACTTAGTAAATCTAAAAACATAAAACAAAATTCAGATGGTCAATAAAAAACTTAAAAAGAACAAAAACGGTAAGAAACTAATACCGGACATCATAGTCGCAAAGATAGAAATAGGATTGTATTGTTCTTAATTAATGAAAATTCTTCCCAGAAGATGATGCTTATAAATGTTCTAAAAGTTTATTCAAAGAAATTATACACGAATGTTGGACTAAGAATATATAGCTCAGTTTTGTTGTTGCTGTTGTTATTACAGTTGAAATTGATGATTAATCATCTTGATATTTGACTGCTAATTAATTTATTTATTGCATCTTTGTACTCGATTTGAGAGATGGATGATTCTTGACAGAGTTTTCATCCATAAGGCTGAGTAACACATTTCTGTGTTCAAAGTAGTCGTCTTAGTCAAGCATTATGGAAAACTTTATGAATGCAATCATCAACAATTGAACTACATTTCCTACTTTACAGCCTTGATCACAATAAGTTGATTGATTAAGTTTTCCGTTCACGGTTACTTCAATGAAATAGTATTTCAGGTAACCTTAATTGCTCAAGGCGACGTTCCTTTGTCTATTGTAATGACAGTATGCACAACTTTAGGAGCCGTAATTTTCACTCCCTTTCTGACAAAGTTTCTAGTAGGAGCTTATATTCCAGTCGACGCTGCACAGCTTTCTCTCAGCACCCTTCAGGTAAAGTTATGTCCACATTTGCCGTCCGGCCATAGTCATGTTCTTAAGTCATCGCCATCGTCTTCTTTTTTGGTTAGGTGGTGGTAGCTCCTATCCTCTTAGGTTCTTGCTTGCAAAAGGCATTTCCTTCCCTGGTGAAATTGGTGTTACCATTTGCACCACTTGTTGCTGTTTTAACTTCCTCGCTGCTTGCTAGCAGGTCTCGGTTCTTCAACAATTGTTTTTTTTTTTTTTTTTTGGCTTCAATAAGTTGTGCTTACCATGGAAACTTGATGAAAAGCCTTTTATTGTTTTTCGCTCTCACACACCTTTGATTATTTTTTTTTTATGATTAGTGTCTTCTCAGAGAATGTCATTCGTATCAAATCGTCAATGGTTAGTGCCACATTGGCCTCTGATGCATCTCTATGGACAGTCCTTAAAAGTATATTATCAGGAGAGCTGGGAGTAGTAATACTTTCGGTGTTTTGTTTACACTTTGCTGGTTTCTTCGTCGGGTAAGTTGCATCTCTTTCCTCACCTCAATGGACTTAGTTGCTCGAATGCAGAAGCTAAACAAATTGTTTACAATATAAATCATTTGAGATGTTACTGAAAGTAATGAACCAAAATGAATACTTAGTCACTGATGAACTGCATAAGATGGATGAAATTTTGCGTTTCCTCCCAAAACAGAATCTTCTCTATGTTGTAGATACTGATTCTTGTTTGCAAGGAGAATATGAAGTCAAATATTGTTATAAAAGATGAAATCAGTCGATTAGCTCACTACACACCCTTACGAGCTTCCTCTGATTTGCAGGTATATAGCAGCTGCTATCTGTGGGTTTCGAGAACGAGAACGAAGAACTATATCCATGCAGGTACAAAACGACATTCACTTATCGAGAAATTGAAAAACATAGATATATGAATGAGAGGGTGTGGATTTTGGCTTCAGGTTGGGATGCAGAATTCATCACTGGGAGTTGTTTTGGCAGCCTCACATTTTAGTTCAGCAATGGTGGCATTGCCTCCAGCAATTTCAGCTGTGATAATGAACATGATGGGGAGCACTCTTGGATTTTGTTGGAAATATATTCAACCTTCTGATGAAGTGAAAACCAGTGTTGTTGCCAAGTGAATTTCTTGTCCTTTTTTTCTTTGTATTTAATTTTACATCTTATTTCCAAATGTAAAAAGAATGTACTTTTAATCTTTAAATTCACTATAGAATCTCCATGCCAATTTCTTCACATCTTATTGTCGTACTTAATAGTCTTTTATGAT

Coding sequence (CDS)

ATGCCTCCGATTTCTCTGCAACTCACCCCCTTCATTTCTCCACTAATCCACCGCCCTAATCTCCGCCTTCACCGACCCCCAATTCCCCCTCTCTCGCCGCCCAGATTACTCACCGTCCGATCGGTTCAACAAAATAACGAACTCCCATCTCCCTCGCCACCGCCAAAACCCACTGGTTTGGACGATTTTCTTTCCACGGCGGCTAGTCTCTACCCTCTCTATGTGACGGCAGGCGGGATTGTTGCTTGCCTCGAACCCTCGACCTTCTCGTGGTTTGTGCAGAGAGGGCCGAGTTCCTATAGCTTATCTCTTGGTTTGATAATGTTGGCCATGGGGCTTACTCTAGAGATCAAGGATTTATTTAATTTGTTCATGCAAAGACCTCTTTCGATATTGTTTGGATGTGTAGCTCAGTACACGATTATGCCAGCTTCTGCAGTGCTGATCGGAAAACTTTTGGGACTTTCCCAGTCCCTTTTGTTCGGTTTGGTCTTGCTTGGATGTTGTCCTGGAGGATCTGCTTCCAATGTGTATGCACAACTTTAG

Protein sequence

MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVYAQL

Homology

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.8e-14
Identity = 45/133 (33.83%), Postives = 75/133 (56.39%), Query Frame = 0


Query: 45  NNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSL 104
           +N+LP+         ++      ++ +P++V+ G ++  + PSTF+W     P+   + L
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGL 134

Query: 105 GLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLV 164
            + ML MG+TL + DL    +  P  +  G + QY++MP SA  + KLL L      GL+
Sbjct: 135 TITMLGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLI 194

Query: 165 LLGCCPGGSASNV 178
           L+GCCPGG+ASN+
Sbjct: 195 LVGCCPGGTASNI 203

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.8e-14
Identity = 55/157 (35.03%), Postives = 83/157 (52.87%), Query Frame = 0


Query: 27  PIPPL------SPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGI 86
           PI P+      S  R++   +   + +LP  S P + +  +  +    +L+PL+V  G +
Sbjct: 56  PISPVFALEATSSRRVVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGTL 115

Query: 87  VACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYT 146
           V   +PS  +W        ++L LG +ML+MGLTL  +D F   ++ P ++  G +AQY 
Sbjct: 116 VGIFKPSLVTWL---ETDLFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYM 175

Query: 147 IMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 178
           I P    LI   L LS  L  GL+L+ CCPGG ASNV
Sbjct: 176 IKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNV 207

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 52/149 (34.90%), Postives = 77/149 (51.68%), Query Frame = 0


Query: 29  PPLSPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPST 88
           P L   +LL       ++ LP  S P +    +  +    +L+P++V  G I+   +PS 
Sbjct: 73  PALRSCQLLCKAEANISSNLPE-SIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSM 132

Query: 89  FSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVL 148
            +W        +++ LG +ML+MGLTL  +D F   M+ P ++  G +AQY I P     
Sbjct: 133 VTWL---ETDLFTVGLGFLMLSMGLTLTFED-FRRCMRNPWTVGVGFLAQYLIKPMLGFA 192

Query: 149 IGKLLGLSQSLLFGLVLLGCCPGGSASNV 178
           I   L LS  L  GL+L+ CCPGG ASNV
Sbjct: 193 IAMTLKLSAPLATGLILVSCCPGGQASNV 216

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 44/112 (39.29%), Postives = 66/112 (58.93%), Query Frame = 0


Query: 67  AASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSY-SLSLGLIMLAMGLTLEIKDLFNLFM 126
           A   + ++V    ++    PS F+W      SSY ++ LG+IM  MGLTL+  D F   +
Sbjct: 11  AGQTFGIWVIVFAVLGFSFPSLFTWI-----SSYITIFLGIIMFGMGLTLQADD-FKELV 70

Query: 127 QRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 178
           ++P  ++ G +AQYTIMP  A  +   L L   +  G++L+GCCPGG+ASNV
Sbjct: 71  RKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNV 116

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 5.9e-12
Identity = 41/111 (36.94%), Postives = 59/111 (53.15%), Query Frame = 0


Query: 71  YPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLS 130
           +P++V +   VA   P  F W     P +  + +   ML MG+TL + DL    +  P  
Sbjct: 106 FPVWVASACAVALWRPPAFLWV---SPMAQIVGISFTMLGMGMTLTLDDLKTALLM-PKE 165

Query: 131 ILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVYAQL 182
           +  G + QY++MP S  LI KLL L      GL+L+ CCPGG+ASN+   L
Sbjct: 166 LASGFLLQYSVMPLSGFLISKLLNLPSYYAAGLILVSCCPGGTASNIVTYL 212

[Close]

BLAST of Cucsat.G241.T16 vs. NCBI nr
Match: XP_004133714.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis sativus] >KGN56248.1 hypothetical protein Csa_010831 [Cucumis sativus])

HSP 1 Score: 339 bits (869), Expect = 1.05e-113
Identity = 175/177 (98.87%), Postives = 175/177 (98.87%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPR LTVRSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRSLTVRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV
Sbjct: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177

[Close]

BLAST of Cucsat.G241.T16 vs. NCBI nr
Match: XP_008452255.1 (PREDICTED: probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis melo] >KAA0060620.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa] >TYK02252.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 321 bits (823), Expect = 9.60e-107
Identity = 163/177 (92.09%), Postives = 170/177 (96.05%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNV
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNV 177

[Close]

BLAST of Cucsat.G241.T16 vs. NCBI nr
Match: XP_038903139.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 282 bits (722), Expect = 2.18e-91
Identity = 152/181 (83.98%), Postives = 160/181 (88.40%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRP-NLRLHRPPIPPLSPPRL---LTVRSVQQNNELPSPSPPPK 60
           M PISLQLTPFISPL+HR  NL L RPPIP LSPP+L   L VRSV +NNE PSPS P K
Sbjct: 1   MAPISLQLTPFISPLLHRRRNLSLRRPPIPSLSPPKLPRSLPVRSVHRNNEHPSPSHPRK 60

Query: 61  PTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLE 120
            TGLDDFLSTAASLYPLYVTAGGIVACL+PSTFSWFVQRGP+SYS SLGLIMLAMGLTLE
Sbjct: 61  STGLDDFLSTAASLYPLYVTAGGIVACLKPSTFSWFVQRGPASYSFSLGLIMLAMGLTLE 120

Query: 121 IKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASN 177
           +KDLFNLFMQRPLSILFGCVAQYTIMPASA LIGK  GLS SLL GL+LLGCCPGG+ASN
Sbjct: 121 LKDLFNLFMQRPLSILFGCVAQYTIMPASAALIGKFFGLSPSLLVGLILLGCCPGGTASN 180

[Close]

BLAST of Cucsat.G241.T16 vs. NCBI nr
Match: XP_022985149.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 272 bits (696), Expect = 1.91e-87
Identity = 143/180 (79.44%), Postives = 154/180 (85.56%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPPKP 60
           M  ISLQ TPFISPL HR NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  TGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEI 120
           +GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTLE+
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 KDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS  L  GL+LL CCPGG+ASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

[Close]

BLAST of Cucsat.G241.T16 vs. NCBI nr
Match: XP_022929395.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 269 bits (688), Expect = 3.27e-86
Identity = 144/182 (79.12%), Postives = 155/182 (85.16%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIH--RPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPP 60
           M  ISLQ TPFISPL H  R NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP 
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 120
           KP+GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSAS 177
           E+KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS SL  GL+LL CCPGG+AS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL
Match: A0A0A0L3A1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G106000 PE=3 SV=1)

HSP 1 Score: 339 bits (869), Expect = 5.08e-114
Identity = 175/177 (98.87%), Postives = 175/177 (98.87%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPR LTVRSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRSLTVRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV
Sbjct: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL
Match: A0A5D3BTX1 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00520 PE=3 SV=1)

HSP 1 Score: 321 bits (823), Expect = 4.65e-107
Identity = 163/177 (92.09%), Postives = 170/177 (96.05%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNV
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNV 177

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL
Match: A0A1S3BSU0 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493327 PE=3 SV=1)

HSP 1 Score: 321 bits (823), Expect = 4.65e-107
Identity = 163/177 (92.09%), Postives = 170/177 (96.05%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPPRLLTVRSVQQNNELPSPSPPPKPTGL 60
           MPPISLQLTPFISPL+HRPNL LHRPPIP LSPPR LT+RSVQQNNE PSPSPPPKPTGL
Sbjct: 1   MPPISLQLTPFISPLLHRPNLCLHRPPIPRLSPPRSLTIRSVQQNNEHPSPSPPPKPTGL 60

Query: 61  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120
           DDFLSTAASLYPLYVTAGGIVAC+EPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL
Sbjct: 61  DDFLSTAASLYPLYVTAGGIVACVEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 120

Query: 121 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           FNLFMQRPLSI+FGCVAQYTIMPASA ++GK LGLSQSLL GL+LLGCCPGGSASNV
Sbjct: 121 FNLFMQRPLSIMFGCVAQYTIMPASAAVVGKFLGLSQSLLSGLILLGCCPGGSASNV 177

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL
Match: A0A6J1JCH6 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483238 PE=3 SV=1)

HSP 1 Score: 272 bits (696), Expect = 9.24e-88
Identity = 143/180 (79.44%), Postives = 154/180 (85.56%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIHRPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPPKP 60
           M  ISLQ TPFISPL HR NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  TGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEI 120
           +GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTLE+
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 KDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 177
           KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS  L  GL+LL CCPGG+ASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

[Close]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL
Match: A0A6J1EUA3 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435979 PE=3 SV=1)

HSP 1 Score: 269 bits (688), Expect = 1.58e-86
Identity = 144/182 (79.12%), Postives = 155/182 (85.16%), Query Frame = 0


Query: 1   MPPISLQLTPFISPLIH--RPNLRLHRPPIPPLSPP---RLLTVRSVQQNNELPSPSPPP 60
           M  ISLQ TPFISPL H  R NLRLHRP IP L PP   RLL VRSVQ+NNE PSP PP 
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 120
           KP+GLDDFLSTAASLYPLYVT GG+VACL+PSTFSWFV+RGP+SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSAS 177
           E+KDL NLFMQRPLSILFGCVAQYTIMPA+  LIGK  GLS SL  GL+LL CCPGG+AS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

[Close]

BLAST of Cucsat.G241.T16 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-15
Identity = 45/133 (33.83%), Postives = 75/133 (56.39%), Query Frame = 0


Query: 45  NNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSL 104
           +N+LP+         ++      ++ +P++V+ G ++  + PSTF+W     P+   + L
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGL 134

Query: 105 GLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLV 164
            + ML MG+TL + DL    +  P  +  G + QY++MP SA  + KLL L      GL+
Sbjct: 135 TITMLGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLI 194

Query: 165 LLGCCPGGSASNV 178
           L+GCCPGG+ASN+
Sbjct: 195 LVGCCPGGTASNI 203

[Close]

BLAST of Cucsat.G241.T16 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 79.3 bits (194), Expect = 3.4e-15
Identity = 55/157 (35.03%), Postives = 83/157 (52.87%), Query Frame = 0


Query: 27  PIPPL------SPPRLLTVRSVQQNNELPSPSPPPKPTGLDDFLSTAASLYPLYVTAGGI 86
           PI P+      S  R++   +   + +LP  S P + +  +  +    +L+PL+V  G +
Sbjct: 56  PISPVFALEATSSRRVVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGTL 115

Query: 87  VACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLFNLFMQRPLSILFGCVAQYT 146
           V   +PS  +W        ++L LG +ML+MGLTL  +D F   ++ P ++  G +AQY 
Sbjct: 116 VGIFKPSLVTWL---ETDLFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYM 175

Query: 147 IMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNV 178
           I P    LI   L LS  L  GL+L+ CCPGG ASNV
Sbjct: 176 IKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNV 207

[Close]

BLAST of Cucsat.G241.T16 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-08
Identity = 41/123 (33.33%), Postives = 62/123 (50.41%), Query Frame = 0


Query: 62  DFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDLF 121
           D +  A S+ P  V A  I+A + P +F+WF  R    +  +LG +M A+G+    KD  
Sbjct: 92  DRMKKANSILPHVVLASTILALIYPPSFTWFTSR---YFVPALGFLMFAVGINSNEKDFL 151

Query: 122 NLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLF--------GLVLLGCCPGGS 177
             F +RP +IL G V QY + P    ++G + GL+   LF        G++L+ C  G  
Sbjct: 152 EAF-KRPKAILLGYVGQYLVKP----VLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQ 206

[Close]

BLAST of Cucsat.G241.T16 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 48.5 bits (114), Expect = 6.5e-06
Identity = 36/125 (28.80%), Postives = 58/125 (46.40%), Query Frame = 0


Query: 56  KPTGLDDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTL 115
           K + + + L  A S  P  +    I+A + P +F+WF    P  +   LG +M A+G+  
Sbjct: 87  KVSSIMETLKQAYSFIPHGILLSTILALVYPPSFTWF---KPRYFVPGLGFMMFAVGINS 146

Query: 116 EIKDLFNLFMQRPLSILFGCVAQYTIMPASAVLIG----KLLGLSQSLLFGLVLLGCCPG 175
             +D     ++RP +I  G + QY I P    + G     L  L  S+  G++L+ C  G
Sbjct: 147 NERDFLEA-LKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSG 206

Query: 176 GSASN 177
              SN
Sbjct: 207 AQLSN 207

The following BLAST results are available for this feature:

BLAST of Cucsat.G241.T16 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93YR2	2.8e-14	33.83	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis tha...	[more]
Q1EBV7	4.8e-14	35.03	Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
Q5VRB2	1.8e-13	34.90	Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su...	[more]
O34524	1.6e-12	39.29	Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 1...	[more]
Q7XVB3	5.9e-12	36.94	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa su...	[more]

BLAST of Cucsat.G241.T16 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133714.1	1.05e-113	98.87	probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis sativus] ...	[more]
XP_008452255.1	9.60e-107	92.09	PREDICTED: probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumi...	[more]
XP_038903139.1	2.18e-91	83.98	probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida...	[more]
XP_022985149.1	1.91e-87	79.44	probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita maxima]	[more]
XP_022929395.1	3.27e-86	79.12	probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita moschat...	[more]

BLAST of Cucsat.G241.T16 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3A1	5.08e-114	98.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G106000 PE=3 SV=1	[more]
A0A5D3BTX1	4.65e-107	92.09	Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BSU0	4.65e-107	92.09	probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX...	[more]
A0A6J1JCH6	9.24e-88	79.44	probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita maxim...	[more]
A0A6J1EUA3	1.58e-86	79.12	probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita mosch...	[more]

BLAST of Cucsat.G241.T16 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78560.1	2.0e-15	33.83	Sodium Bile acid symporter family	[more]
AT2G26900.1	3.4e-15	35.03	Sodium Bile acid symporter family	[more]
AT4G22840.1	1.1e-08	33.33	Sodium Bile acid symporter family	[more]
AT4G12030.2	6.5e-06	28.80	bile acid transporter 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038770	Sodium/solute symporter superfamily	GENE3D	1.20.1530.20		coord: 58..181 e-value: 7.3E-28 score: 99.5
IPR002657	Bile acid:sodium symporter/arsenical resistance protein Acr3	PFAM	PF01758	SBF	coord: 102..181 e-value: 1.5E-16 score: 60.7
IPR004710	Bile acid:sodium symporter	PANTHER	PTHR10361	SODIUM-BILE ACID COTRANSPORTER	coord: 15..178
None	No IPR available	PANTHER	PTHR10361:SF58	SODIUM/METABOLITE COTRANSPORTER BASS2, CHLOROPLASTIC-RELATED	coord: 15..178

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G241	Cucsat.G241	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G241.T16.E1	Cucsat.G241.T16.E1	exon
Cucsat.G241.T16.E2	Cucsat.G241.T16.E2	exon
Cucsat.G241.T16.E3	Cucsat.G241.T16.E3	exon
Cucsat.G241.T16.E4	Cucsat.G241.T16.E4	exon
Cucsat.G241.T16.E5	Cucsat.G241.T16.E5	exon
Cucsat.G241.T16.E6	Cucsat.G241.T16.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G241.T16.C3	Cucsat.G241.T16.C3	CDS
Cucsat.G241.T16.C2	Cucsat.G241.T16.C2	CDS
Cucsat.G241.T16.C1	Cucsat.G241.T16.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G241.T16	Cucsat.G241.T16-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane