Cucsat.G2253.T17 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G2253.T17
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Acetamidase/Formamidase family protein
Location	ctg1002: 2167773 .. 2171768 (+)
RNA-Seq Expression	Cucsat.G2253.T17
Synteny	Cucsat.G2253.T17

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAGCTATTTGGTATTTCGAAGGAATCTATGCATACTCTCCTCAAATCCCAGGTAAATGTTACAACTTTAAACAATGCTTCCAATAAGAATGTGTAGCATAGATGCATTAGCAACCATTATATGCCAATACAAAGAACCCCAGATATATTGAGATGTAAAAGATGACGAAACTACAAAATAAATTATGCAATTCAAGGTACTTGGACCCTCCCGATCTTTTGAGATCACTCTCAAGCCATGATTCTCAAGCCAAAATATTACCCCGTTTTTCTCCTTCTCCACTCGATGTATTTGTATCAAAACTCCCCTAACAAACTACTTAACTAATTAATAATATACCCTCAATATCCATCTAATACCACTTCTGTCATACGCCTTCCAGGTGTTCGATTCCCAGGCTTAACTCATCCTGGAGTAATAGGAACTGCTCCATCGGCTGAACTTTTGAAAATTTGGAATGAAAGGGAAAGAGATGTTCAAGAAAATGGCCTTCAAACTCTAAAGCTTTGTGAAGTTGTGCATTCACGACCCTTAGCAAACCTACCATCGGCTAAAGGTTGCGCTCTTGGAAAGGTATGATCTTAAACTAACATTTTCGTTAGATGCATAAAACATAAAACTCCAATAAATTTATGTAAACCTTCCAAATTCCACACAAAATAATGGCCTTGCTGATTTTCCCTCCAATTCAAGTGAAGTGTATTATGTAGTTTTCTAACATTGCATTGCATTCTACATTGCTTCAAACTAGATCAAAGAGGGGACAGAAGAATGGGAAAGAATTGCTAATGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGAAATTGTGATATCAAAAACCTCAGTAGAGGTTCAAAAGTATACCTTCCAGTATTTGTGGAAGGAGCAAACTTAAGCTCAGGTGACATGCACTTCTCCCAAGGGGATGGTGAAGTCTCATTCTGTGGAGCAATTGAAATGAGTGGTTTTCTAGAACTCAAGTAAAGTTCTCAGCTTGGCTTGGTTTCCTTTTCATTTTCTTTTCCTACTTCAACAATTAATTATATACAAATATTACTTGTGTTATATGTTCTTGTTCTTCAGGTGTGATATTATAAGGGGTGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTGAACCCAATTTTTGAGATAGGTCCAGTTGAACCAAGATTCTCAGAGTGGTTGGTTTTTGAAGGCATCAGCGTTGATGAAAGTGGGAGGCAGCATTTTCTTGATGCCACTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATTGACTACCTTCACAAATTTGGGTACTCAAAGGAGCAGGTATGGTGACTTTCTTCGATTGCCATTGGTAGTACGAGAACTCACACAAGCAAATTCACAAACACCTTACACGAGCAAAAAAACTAAATAACAATGGCTGTGTAATACGATTATTGAAAATAAGATGTGTGAAATTCAGGCTTATCTGCTGCTATCGTGCTGCCCATGTGAAGGAAGAATCTCTGGGATTGTTGATACGCCTAATGCCATGGTTACCTTGGCAATTCCCACCGCCATTTTCGACCAGGTACCTACCTTTCTTCGTCTCCTCGTATGGGTTGGTTTAATTTCATCAAGTTATTGCAAAATCACCACCTACATAGTTTTAGTCCTTCAAAATCTATTTCAGTGGTGAGAAAAGTACATTGGAAAACGTAAAATCAAACTGAGTTATTAAATGAATGTGTTGGAAATCATTTTGATTTTGAGAAAAGTGATTTTTTTCAAAATCATTCCTGAACATGTAAAGGATAACAGCATTGTGTTAATCACTATAGCACAAATGAATTACATTAATTGCTGGGTGACTATAACTTGTGGATCACAAATCCTTGAAACAAATCTAGAATGGTTAAAAACATTTGGACATTTTCAAAATCACTTGGGGATATGCTTTTCAACCTTGGAAATCAATTTTGGTAATGTTTAAATTATTAAAATGATATATTGTATCAATCTTAAATATGATAAAAGTGATTTTAACGATTTTAAATATGATAAAAGTGATTTTAACGATTTTAAATTATTCTCACACTAACCAGTACATTACAATCAAATTAAACGTGTGAATTTGACCATTTGCTTTTGTTATTCCCTTTTACTAAAACTACTTTTCTGCTCCCTAGTGATAAGAAAGCCATAGTCCATAGTCCAACATTTGCTTTTAGTATTTTAAAAAACAATCGAATATCATAGATTCGCATGAGAATATTGAAGCGAATATTTATGGATAACATGAAAAGGTGAATGTAATGTGTAGACTTCTAAATCAATTTCAAAATGCTGACCACATCTCAAGTCAATAAACATGTTTTTCATCATATAATATAAATTTCATCATATAATATAAATTAAGGAGGTACTACACATACTTACCTCATAGTATTAAATAACTCAATTACTAATGAAGTTGTTGAAAAACTTCATCATTACACATTTACAAACTTCTTGACTTAGATTATGATTATTTTAGTTTGAATGATAATAGTATGTGTTTATAATGTAAAATATTTTAATTTGATAAGAAAATAGTGAAGGTCATCGCAAATAATAAAAGAAATGATGAAGGTCAAATAATAAAAACGATAGTAGATGAGAGATCTTGAAACAGTGTAGATAGAAATGGTTATTATAGTTTTGGATAATCTTAATCCCATTCGGAGATAGTTAATATTATCTAAAATCAAATGTATTAATCTCTGTCCGTAAGTTTGTTTAATATGTATAAAGAAAAATGTGTGAATGAGCAGGACATTCGCCCAAAACCGAGCAAAGTTCCTACTGGACCTCGACTATTAAGAAGACCAGACGTCCTGAAATGTAGCTACGATGGGAATTTGCCCATCACAAAGAACCCCAGCGCCTCACCATAAAACATGTGTGGTTCTTTTCAGTTTCTCTTTTCTCTTTTCTTTTTTATAATTTCAGAGAGAGAATAACCCAATACTGTTTCAATAAAGTAATTGTCGTTTGACTGTCTGTAACAGTACTAGAGTAAAAGATATACGAATCTAAAATTTGTAATATTGGGTCCTTTGATACTAAACTTCAGAGTTACTAAAAGAGATGCCTCAAGAAATCACCTCGAGCATCATCCGCCAAAATTTTGCCATTCTCTAACTGGCTGGTGCATAACAGAAAGCTATCAGATCAAATGTTGGTTGGAATCAATAGTAAATTGAAAGAGTACGATGGTGTGTCAGTGATGGACACTGGATTCCAAAGGACCAAAGCCAAAGATCTAACTCCACGCTTAGAACCTGCAAAAATCCAAATCTTTTTACTCTATAGTTTGGAAAACCAAAGCATGGGCAGAAGGTAGAGGGCTAGAGATGGGATATGTCATTTTTTCCGGTTTTTGTCAGCTGCCAGAAAGTAAATATACTTACAGACGTAAAATTGAATCCTACAACAGTGGAGTAGAACTATGGAGTGAAAGTCGTCTAAAGATAATTTAATAAGGAAATACCTGTTTTCCTGATTTCATACTAATCAATTCTCTCATTTTTTCAACTGCATATATTGAGCAAGCTCGGAGTTCTACTTCCTCCTCGCTGCCTGGACCAATTTCACTGTTAGCATCGATGATGCTTGCAAGTGTAGAACTGTATTTTAGCACCCCAAGCTGTCGAAGTACAGCAGGAACGATGTAGTCTGCCATAATGGTGATTGAACCAATGTCATTGAACTCTCCATATCCTTGTCCATTGAATGCACCCCAAAGGTCAGCTGCAAATATTTGAGCTCGTTTGTACAGGAATACCTGGTGGCCTTTGTAAAGAGAGTGATCTCGAAAGCCTGATGTAAGGCGATGAACATATCAAACGACCATCTCAATATATTGTCATTAACATTAGCACCTAATTCTAATAAATAACATGTTAAATAAAATTATATAAAAGTTGATACCAGGAAAGTGTTGTGTGATAACAGCAACAAGC

Coding sequence (CDS)

ATGTTCTTGTTCTTCAGGTGTGATATTATAAGGGGTGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTGAACCCAATTTTTGAGATAGGTCCAGTTGAACCAAGATTCTCAGAGTGGTTGGTTTTTGAAGGCATCAGCGTTGATGAAAGTGGGAGGCAGCATTTTCTTGATGCCACTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATTGACTACCTTCACAAATTTGGGTACTCAAAGGAGCAGGCTTATCTGCTGCTATCGTGCTGCCCATGTGAAGGAAGAATCTCTGGGATTGTTGATACGCCTAATGCCATGGTTACCTTGGCAATTCCCACCGCCATTTTCGACCAGGACATTCGCCCAAAACCGAGCAAAGTTCCTACTGGACCTCGACTATTAAGAAGACCAGACGTCCTGAAATGTAGCTACGATGGGAATTTGCCCATCACAAAGAACCCCAGCGCCTCACCATAA

Protein sequence

MFLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFDQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP

Homology

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy Swiss-Prot
Match: Q50228 (Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.9e-32
Identity = 68/143 (47.55%), Postives = 94/143 (65.73%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           +L  +  +I+ G+K+Y           NP+F+  P+ P + ++++FEGISVDE+G+QH+L
Sbjct: 271 YLDIKVGLIKDGVKKYGIK--------NPVFQPSPITPTYRDYMIFEGISVDEAGKQHYL 330

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           D  +AY++A LNAI+YL KFGYS EQA  +L   P EG ISGIVD PNA  TL IPT IF
Sbjct: 331 DVHIAYRQACLNAIEYLKKFGYSGEQAVSILGTAPVEGHISGIVDIPNACATLWIPTEIF 390

Query: 122 DQDIRPKPSKVPTGPRLLRRPDV 145
           + DIRP       GP+++  P V
Sbjct: 391 EFDIRPNAD----GPKIMVPPGV 401

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy Swiss-Prot
Match: Q9URY7 (Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.04 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.5e-26
Identity = 54/124 (43.55%), Postives = 78/124 (62.90%), Query Frame = 0


Query: 6   RCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRF--SEWLVFEGISVDESGRQHFLDA 65
           +C I++ G+ +            +P++  GPVEP F  S +L FEG SVDESG+QH+L  
Sbjct: 278 KCKILKNGISDLAMK--------SPMYLPGPVEPHFSPSRYLTFEGFSVDESGKQHYLCT 337

Query: 66  TVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFDQ 125
           T AY++  L  I+Y  +FGY+  Q YLLLSC P +G ++GIVD PN+  T+ +P  IF+ 
Sbjct: 338 TTAYRQTCLRVIEYFRRFGYNDYQLYLLLSCAPIQGHVAGIVDIPNSCTTIGVPMDIFEF 393

Query: 126 DIRP 128
           D+ P
Sbjct: 398 DVSP 393

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy Swiss-Prot
Match: Q07838 (Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 6.9e-20
Identity = 52/99 (52.53%), Postives = 66/99 (66.67%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDES-GRQHF 61
           F+    D+I+GGM+ Y    G T    NPIF  G VEP +SEWL F GISVD +  R  +
Sbjct: 279 FIDMHVDLIKGGMETY----GVT---TNPIFMPGRVEPLYSEWLTFIGISVDHAENRNAY 338

Query: 62  LDATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEG 100
           +DAT+AY+ A LNAI+YL K+GY+ EQAYL+L   P EG
Sbjct: 339 MDATMAYRNACLNAIEYLKKWGYTGEQAYLILGTSPIEG 370

[Close]

BLAST of Cucsat.G2253.T17 vs. NCBI nr
Match: XP_004141873.1 (uncharacterized protein LOC101206142 isoform X2 [Cucumis sativus])

HSP 1 Score: 335 bits (859), Expect = 7.50e-112
Identity = 159/162 (98.15%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 410

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 163
           DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP
Sbjct: 411 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 452

[Close]

BLAST of Cucsat.G2253.T17 vs. NCBI nr
Match: XP_011657890.1 (uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hypothetical protein Csa_003261 [Cucumis sativus])

HSP 1 Score: 335 bits (859), Expect = 7.97e-112
Identity = 159/162 (98.15%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 412

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 163
           DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP
Sbjct: 413 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 454

[Close]

BLAST of Cucsat.G2253.T17 vs. NCBI nr
Match: XP_008440368.1 (PREDICTED: formamidase-like isoform X3 [Cucumis melo])

HSP 1 Score: 328 bits (840), Expect = 5.64e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 410

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 411 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 451

[Close]

BLAST of Cucsat.G2253.T17 vs. NCBI nr
Match: XP_008440367.1 (PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 328 bits (840), Expect = 6.00e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 413 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 453

[Close]

BLAST of Cucsat.G2253.T17 vs. NCBI nr
Match: XP_008440366.1 (PREDICTED: formamidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 328 bits (840), Expect = 6.19e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 294 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 353

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 354 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 413

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 414 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 454

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL
Match: A0A0A0KGH0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1)

HSP 1 Score: 335 bits (859), Expect = 3.86e-112
Identity = 159/162 (98.15%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 412

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 163
           DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP
Sbjct: 413 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSASP 454

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL
Match: A0A1S3B0Y4 (formamidase-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 328 bits (840), Expect = 2.73e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 410

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 411 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 451

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL
Match: A0A5D3CLP5 (Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004150 PE=4 SV=1)

HSP 1 Score: 328 bits (840), Expect = 2.90e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 413 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 453

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL
Match: A0A1S3B102 (formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 328 bits (840), Expect = 2.90e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 413 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 453

[Close]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL
Match: A0A1S3B0J2 (formamidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 328 bits (840), Expect = 2.99e-109
Identity = 155/161 (96.27%), Postives = 158/161 (98.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 294 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 353

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 354 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 413

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 162
           DQDIRPKPSKVP GPRLLRRPDVLKCSYDGNLPITKNPSA+
Sbjct: 414 DQDIRPKPSKVPIGPRLLRRPDVLKCSYDGNLPITKNPSAT 454

[Close]

BLAST of Cucsat.G2253.T17 vs. TAIR 10
Match: AT4G37550.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-80
Identity = 137/161 (85.09%), Postives = 150/161 (93.17%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVD+PNA+ TLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIF 410

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 163
           DQDIRPK  KVP GPR++R+PDVLK +YDG LPITKNPS+S
Sbjct: 411 DQDIRPKNRKVPVGPRVVRKPDVLKSTYDGKLPITKNPSSS 451

[Close]

BLAST of Cucsat.G2253.T17 vs. TAIR 10
Match: AT4G37550.3 (Acetamidase/Formamidase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-80
Identity = 137/161 (85.09%), Postives = 150/161 (93.17%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 271 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 330

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVD+PNA+ TLAIPTAIF
Sbjct: 331 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIF 390

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 163
           DQDIRPK  KVP GPR++R+PDVLK +YDG LPITKNPS+S
Sbjct: 391 DQDIRPKNRKVPVGPRVVRKPDVLKSTYDGKLPITKNPSSS 431

[Close]

BLAST of Cucsat.G2253.T17 vs. TAIR 10
Match: AT4G37560.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-78
Identity = 135/161 (83.85%), Postives = 148/161 (91.93%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPT LHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTTLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGRISGIVD+PNA+ TLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIF 410

Query: 122 DQDIRPKPSKVPTGPRLLRRPDVLKCSYDGNLPITKNPSAS 163
           DQDIRPK  KVPTG R++++PDV+K +YDG LPITKN S+S
Sbjct: 411 DQDIRPKTRKVPTGARIVKKPDVMKSTYDGKLPITKNSSSS 451

[Close]

BLAST of Cucsat.G2253.T17 vs. TAIR 10
Match: AT4G37550.2 (Acetamidase/Formamidase family protein )

HSP 1 Score: 168.3 bits (425), Expect = 5.1e-42
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQ 88
           DATVAYKRAVLNAIDYL KFGYSKEQ
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQ 376

The following BLAST results are available for this feature:

BLAST of Cucsat.G2253.T17 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50228	7.9e-32	47.55	Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1	[more]
Q9URY7	6.5e-26	43.55	Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]
Q07838	6.9e-20	52.53	Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2	[more]

BLAST of Cucsat.G2253.T17 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141873.1	7.50e-112	98.15	uncharacterized protein LOC101206142 isoform X2 [Cucumis sativus]	[more]
XP_011657890.1	7.97e-112	98.15	uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hy...	[more]
XP_008440368.1	5.64e-109	96.27	PREDICTED: formamidase-like isoform X3 [Cucumis melo]	[more]
XP_008440367.1	6.00e-109	96.27	PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-li...	[more]
XP_008440366.1	6.19e-109	96.27	PREDICTED: formamidase-like isoform X1 [Cucumis melo]	[more]

BLAST of Cucsat.G2253.T17 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGH0	3.86e-112	98.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1	[more]
A0A1S3B0Y4	2.73e-109	96.27	formamidase-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]
A0A5D3CLP5	2.90e-109	96.27	Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3B102	2.90e-109	96.27	formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]
A0A1S3B0J2	2.99e-109	96.27	formamidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]

BLAST of Cucsat.G2253.T17 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37550.1	1.2e-80	85.09	Acetamidase/Formamidase family protein	[more]
AT4G37550.3	1.2e-80	85.09	Acetamidase/Formamidase family protein	[more]
AT4G37560.1	1.2e-78	83.85	Acetamidase/Formamidase family protein	[more]
AT4G37550.2	5.1e-42	89.53	Acetamidase/Formamidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004304	Acetamidase/Formamidase	PFAM	PF03069	FmdA_AmdA	coord: 5..123 e-value: 1.3E-43 score: 149.3
IPR004304	Acetamidase/Formamidase	PANTHER	PTHR31891	FORMAMIDASE C869.04-RELATED	coord: 2..161
None	No IPR available	GENE3D	3.10.28.20		coord: 53..123 e-value: 7.3E-7 score: 31.0
None	No IPR available	PANTHER	PTHR31891:SF8	FORMAMIDASE-RELATED	coord: 2..161
None	No IPR available	SUPERFAMILY	141130	Acetamidase/Formamidase-like	coord: 4..122

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G2253	Cucsat.G2253	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2253.T17.E1	Cucsat.G2253.T17.E1	exon
Cucsat.G2253.T17.E2	Cucsat.G2253.T17.E2	exon
Cucsat.G2253.T17.E3	Cucsat.G2253.T17.E3	exon
Cucsat.G2253.T17.E4	Cucsat.G2253.T17.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2253.T17.C1	Cucsat.G2253.T17.C1	CDS
Cucsat.G2253.T17.C2	Cucsat.G2253.T17.C2	CDS
Cucsat.G2253.T17.C3	Cucsat.G2253.T17.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2253.T17	Cucsat.G2253.T17-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides