Cucsat.G2253 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G2253
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Acetamidase/Formamidase family protein
Location	ctg1002: 2164431 .. 2170629 (+)
RNA-Seq Expression	Cucsat.G2253
Synteny	Cucsat.G2253

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGCGGATGACGTGGCTGACATCGACTAATGAGGATGCTGAGGTGGCTTTCTTATCTTCTCCCAAAATTTCTACTTCAATTCCCTTCCTACATTACATTACCAATTACCATTCTTCTATCACATCTTCATCTTCCATACACTCATGAATTATCTCACAATACCTATATTTCATTGCTTTACGTTTCTTATATTACCAAACAATATTTACAATATAAAGAAGAAGAAAAACCACCGTTTCAAGTCGTCATCTTCTCAAGTTTTGTCTCAACATTTTGATAATTTGCTACTTCAAAAATAGTTTTTAAAGGAATAGAGCAATTTCACATTTCATCTACACTGTGTCGGCTAAAAGTATGATTATACAAATATCAACTATATACTTGTCAGGAGTTTCACAAGAGATAATTATATTGACCTCTTGAACAACCTCTCCCTTTTATCTATCAATTATCAATTATCAATTTGGTTTCTACATTCCAATACATTGATTTCAATTGGATATGAGTACTGTAGTCAGTACACACCATGTTAGTCCAGGTGTCAACTCTTTTATTAATTATTAATATTGTATTCATTAACCAAAAAACAAATATTATAAGTTAACATATAGGATATATCAGTTGAAAGCTTTCATATTCGACATAATTGACATAAATTTGAAAATGCATGAGCAAAAGAACAAAACAAACCAGTCTATTCTAAACCAACAGTACTCTTCTTTGAAAAAGGAGCATGAAAACTTACTAACTGTTGAGCTCTACACAAAGCAGTCTATTCTAAATGTAAAGAGCTTAAGTAGAAGATTTGACTTTTGTTAATGTTGAGTAATGAAGTAATTATCAGTATGATGGACAACACATGCCACAAATCTCACTGAAATTGCCTTCTTTCGCTCCACAGTGTAAAGAGGTTGATGGACCATTTGGATTTACTTCCCAAAACTGTTTTTTTTTTAAATCCTTTCTTATTTAAACTCTTTTATATATATATAAAAAAATGACTTATGTTTTAGGTTGAGAATATATTCCAAACATACTTTAAGTTTCTATCACTGTCATTTTTGTTTAGTAAATAGTATTTGACGGCTATGAATTTTGCTTGTTCTAAAAATATCCCAATCATTCCTGTTTACGTTCAGTACTTTTTCTTATGAAAAATATTCCTTTGAAAAATGTATAACAATATGGTGAAATTGAGTTTTGTATATTTTCAGGTGTCTTGTTTGTGAGAAGAAGAAAGTAATTACTAGTAATAATCAAATGGATCACATCTTAATTTAAGGACTGTGAATATAGATATCATAAATGAAAACTGAACTTTAATGAGTATGATGTATGATATTTAGATAATTAATAAGCAAATAAAAATAATTCGGTGAAGAAATTAATGTGCAATGGGAAAAATGTCATTGTAATAATATCATAATGTCATGAGTTGATGGTTGTGATCAAAATTTGTAAGGCCAAGCTGATGGGACAGTGGAGATCATAAAATCTTTTATTTCACTGCACCCAAATCCTCTAAAGATTCCAACTATGGTCCATGGATGGATGTGACCTCTTTTTTTCTCTCTCCCTTTCCCTTGTTTCACACAACTTAAAGTTATATATTGGTTCAATGACAACAGGTCTAGTTTAAAATCTCTTTCAGAGAAAATCTGAAAACAAGAAATTCAACCAAAAGTGTTTCATATTTTATTTATAAATGATAAAAGTTGTTGTAGTTATCAACCAAATGTTAAGTTATCTATAAACTAATATTAAGATTCCATTTGCAACCACTTTGTTTTTAATTTTTTGGTTTTTAGTAAAGAAGTTTATTTCATCCTAGCTTCTTGGGATAATTTTTCTATTTCTTGAAGTAAAATAGTTAGAATTTGAGAAAGCAAACTTTTTTCTTATTAGAAAAAAAGTGGTTTCAATATAGTATATTTTGAATCTTTCCTATTAGAATTTAGACAACGACGTATATTTTATAGTATGTTTTTATTAGATTAGATTAATTAGACAAAAACCGAGAGACGCAAGTCGCCAGTAGTCCACATCGTACCAAGGAAAAACTCTCCATTACCTTGTAATTGTACTGTTTTAGTTCCACTAACGAAAACATAGAAAAAAAGGAGGAAGAAGAAGAAGGATATGGGTGATTCTGGTTATGGTCCAAGACTAGTGGTGCCTATAGATTTGAAGAAGAAGCCATGGCAACAGGAATTTCCACTTCACAACCGGTGGCACCCGGAGATACCGCCGGTGGCAGAGGTTAAAGTGGCTGAGTTTTTCAGAGTAGAAATGGTGGATTTTAGCGGCGGAGGAATCACCCAAGACTACTCTGCTGATGACATTAAACATGTTGATAACTCTGTTGTAAGTTCTCTTCCTCTCTCACCCTCATCTTTGTCTCATCCCCAACTTTTGTATTGCTAAATAATCACACCTCAGCTAGTTTACAGTACAAAATAAAAGGAGCGAGATTCTCAACGTAACTCTAGGAGATCGAAAGATTCCATTACTTGGTTGTCAACGTCCATGATGAATAAGAAAGACAAACTAGCGTCTTATCATTTGTCTTATCTTTTTATCTTTTTGTGTACTTTTCAACAATTTCTGCTGCTTAGATTAGAAGGGACTGTTCAATAAAAGAACTTAGTAGAACAAAACTTACGTTCCTGACCATGTTAAGCCCCTAGTTTCTGTCTACTTCTTGTCCAATTTCATGGCTCCTTCTACTCCAAGACTTGTTGTTCCGGTAGACTTGAAAAAGAAGCCATGGGATCAAATTTTACCTCTTCACAACCGTTGGCACCCGCTTATACCCCCAGTTGCTGAGGTTTACACGGATGAGCTCTTTCGGGTTGAGATGGTGGACTGGACTGGAGGAGTTATTGGAGATAATGACTCTGCTAGTGATGTACAGAAAATTGACCTTTCTGTTGTAAGTATAAATATTATTAACCTTATCTATTCTTATGGGAGATAAAAATTTAGGAATAGTTGCCTTTTCGAGCTCCTAATTAGCCTAGTCTAATAATTGGTAAAGCTTTCTACTCAGTACAGTCATAGTTAACTAGTACAATGATGAACAGTGAGTTCTAAAGCTAATACAAAGAATGTAGATTTCAATATACTAATAATGTGCTGTTATTTCTAAAATGTCTAGACCCATTATTTGAGTGGCCCAATTAGGGTTGTCGACAAAGATGGAGTTCCAGCCAAGCCAGGGGATCTACTTGTAGTCGAGATTTGCAACTTGGGTCCTCTCCCAGGAGATGAATGGGGTTATACTGCAACATTTGACAGAGAAAATGGAGGTGGTTTTCTCACCGACCATTTTCCTTGTGCAACCAAAGCTATTTGGTATTTCGAAGGAATCTATGCATACTCTCCTCAAATCCCAGGTAAATGTTACAACTTTAAACAATGCTTCCAATAAGAATGTGTAGCATAGATGCATTAGCAACCATTATATGCCAATACAAAGAACCCCAGATATATTGAGATGTAAAAGATGACGAAACTACAAAATAAATTATGCAATTCAAGGTACTTGGACCCTCCCGATCTTTTGAGATCACTCTCAAGCCATGATTCTCAAGCCAAAATATTACCCCGTTTTTCTCCTTCTCCACTCGATGTATTTGTATCAAAACTCCCCTAACAAACTACTTAACTAATTAATAATATACCCTCAATATCCATCTAATACCACTTCTGTCATACGCCTTCCAGGTGTTCGATTCCCAGGCTTAACTCATCCTGGAGTAATAGGAACTGCTCCATCGGCTGAACTTTTGAAAATTTGGAATGAAAGGGAAAGAGATGTTCAAGAAAATGGCCTTCAAACTCTAAAGCTTTGTGAAGTTGTGCATTCACGACCCTTAGCAAACCTACCATCGGCTAAAGGTTGCGCTCTTGGAAAGGTATGATCTTAAACTAACATTTTCGTTAGATGCATAAAACATAAAACTCCAATAAATTTATGTAAACCTTCCAAATTCCACACAAAATAATGGCCTTGCTGATTTTCCCTCCAATTCAAGTGAAGTGTATTATGTAGTTTTCTAACATTGCATTGCATTCTACATTGCTTCAAACTAGATCAAAGAGGGGACAGAAGAATGGGAAAGAATTGCTAATGAGGCTGCAAGAACAATTCCAGGAAGAGAAAATGGTGGAAATTGTGATATCAAAAACCTCAGTAGAGGTTCAAAAGTATACCTTCCAGTATTTGTGGAAGGAGCAAACTTAAGCTCAGGTGACATGCACTTCTCCCAAGGGGATGGTGAAGTCTCATTCTGTGGAGCAATTGAAATGAGTGGTTTTCTAGAACTCAAGTAAAGTTCTCAGCTTGGCTTGGTTTCCTTTTCATTTTCTTTTCCTACTTCAACAATTAATTATATACAAATATTACTTGTGTTATATGTTCTTGTTCTTCAGGTGTGATATTATAAGGGGTGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTGAACCCAATTTTTGAGATAGGTCCAGTTGAACCAAGATTCTCAGAGTGGTTGGTTTTTGAAGGCATCAGCGTTGATGAAAGTGGGAGGCAGCATTTTCTTGATGCCACTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATTGACTACCTTCACAAATTTGGGTACTCAAAGGAGCAGGTATGGTGACTTTCTTCGATTGCCATTGGTAGTACGAGAACTCACACAAGCAAATTCACAAACACCTTACACGAGCAAAAAAACTAAATAACAATGGCTGTGTAATACGATTATTGAAAATAAGATGTGTGAAATTCAGGCTTATCTGCTGCTATCGTGCTGCCCATGTGAAGGAAGAATCTCTGGGATTGTTGATACGCCTAATGCCATGGTTACCTTGGCAATTCCCACCGCCATTTTCGACCAGGTACCTACCTTTCTTCGTCTCCTCGTATGGGTTGGTTTAATTTCATCAAGTTATTGCAAAATCACCACCTACATAGTTTTAGTCCTTCAAAATCTATTTCAGTGGTGAGAAAAGTACATTGGAAAACGTAAAATCAAACTGAGTTATTAAATGAATGTGTTGGAAATCATTTTGATTTTGAGAAAAGTGATTTTTTTCAAAATCATTCCTGAACATGTAAAGGATAACAGCATTGTGTTAATCACTATAGCACAAATGAATTACATTAATTGCTGGGTGACTATAACTTGTGGATCACAAATCCTTGAAACAAATCTAGAATGGTTAAAAACATTTGGACATTTTCAAAATCACTTGGGGATATGCTTTTCAACCTTGGAAATCAATTTTGGTAATGTTTAAATTATTAAAATGATATATTGTATCAATCTTAAATATGATAAAAGTGATTTTAACGATTTTAAATATGATAAAAGTGATTTTAACGATTTTAAATTATTCTCACACTAACCAGTACATTACAATCAAATTAAACGTGTGAATTTGACCATTTGCTTTTGTTATTCCCTTTTACTAAAACTACTTTTCTGCTCCCTAGTGATAAGAAAGCCATAGTCCATAGTCCAACATTTGCTTTTAGTATTTTAAAAAACAATCGAATATCATAGATTCGCATGAGAATATTGAAGCGAATATTTATGGATAACATGAAAAGGTGAATGTAATGTGTAGACTTCTAAATCAATTTCAAAATGCTGACCACATCTCAAGTCAATAAACATGTTTTTCATCATATAATATAAATTTCATCATATAATATAAATTAAGGAGGTACTACACATACTTACCTCATAGTATTAAATAACTCAATTACTAATGAAGTTGTTGAAAAACTTCATCATTACACATTTACAAACTTCTTGACTTAGATTATGATTATTTTAGTTTGAATGATAATAGTATGTGTTTATAATGTAAAATATTTTAATTTGATAAGAAAATAGTGAAGGTCATCGCAAATAATAAAAGAAATGATGAAGGTCAAATAATAAAAACGATAGTAGATGAGAGATCTTGAAACAGTGTAGATAGAAATGGTTATTATAGTTTTGGATAATCTTAATCCCATTCGGAGATAGTTAATATTATCTAAAATCAAATGTATTAATCTCTGTCCGTAAGTTTGTTTAATATGTATAAAGAAAAATGTGTGAATGAGCAGGACATTCGCCCAAAACCGAGCAAAGTTCCTACTGGACCTCGACTATTAAGAA

Coding sequence (CDS)

ATGTTCTTGTTCTTCAGGTGTGATATTATAAGGGGTGGAATGAAAGAGTACCTGACACCAATGGGGCCGACTCCTCTGCACGTGAACCCAATTTTTGAGATAGGTCCAGTTGAACCAAGATTCTCAGAGTGGTTGGTTTTTGAAGGCATCAGCGTTGATGAAAGTGGGAGGCAGCATTTTCTTGATGCCACTGTAGCTTACAAGCGCGCTGTGCTCAACGCCATTGACTACCTTCACAAATTTGGGTACTCAAAGGAGCAGGCTTATCTGCTGCTATCGTGCTGCCCATGTGAAGGAAGAATCTCTGGGATTGTTGATACGCCTAATGCCATGGTTACCTTGGCAATTCCCACCGCCATTTTCGACCAGGTACCTACCTTTCTTCGTCTCCTCGTATGGGTTGGTTTAATTTCATCAAGTTATTGCAAAATCACCACCTACATAGTTTTAGTCCTTCAAAATCTATTTCAGTGGTGA

Protein sequence

MFLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFLDATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFDQVPTFLRLLVWVGLISSSYCKITTYIVLVLQNLFQW

Homology

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BLAST of Cucsat.G2253 vs. ExPASy Swiss-Prot
Match: Q50228 (Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-28
Identity = 60/121 (49.59%), Postives = 83/121 (68.60%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           +L  +  +I+ G+K+Y           NP+F+  P+ P + ++++FEGISVDE+G+QH+L
Sbjct: 271 YLDIKVGLIKDGVKKYGIK--------NPVFQPSPITPTYRDYMIFEGISVDEAGKQHYL 330

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           D  +AY++A LNAI+YL KFGYS EQA  +L   P EG ISGIVD PNA  TL IPT IF
Sbjct: 331 DVHIAYRQACLNAIEYLKKFGYSGEQAVSILGTAPVEGHISGIVDIPNACATLWIPTEIF 383

Query: 122 D 123
           +
Sbjct: 391 E 383

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BLAST of Cucsat.G2253 vs. ExPASy Swiss-Prot
Match: Q9URY7 (Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.04 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.6e-24
Identity = 52/119 (43.70%), Postives = 75/119 (63.03%), Query Frame = 0


Query: 6   RCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRF--SEWLVFEGISVDESGRQHFLDA 65
           +C I++ G+ +            +P++  GPVEP F  S +L FEG SVDESG+QH+L  
Sbjct: 278 KCKILKNGISDLAMK--------SPMYLPGPVEPHFSPSRYLTFEGFSVDESGKQHYLCT 337

Query: 66  TVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIFD 123
           T AY++  L  I+Y  +FGY+  Q YLLLSC P +G ++GIVD PN+  T+ +P  IF+
Sbjct: 338 TTAYRQTCLRVIEYFRRFGYNDYQLYLLLSCAPIQGHVAGIVDIPNSCTTIGVPMDIFE 388

[Close]

BLAST of Cucsat.G2253 vs. ExPASy Swiss-Prot
Match: Q07838 (Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 6.7e-20
Identity = 52/99 (52.53%), Postives = 66/99 (66.67%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDES-GRQHF 61
           F+    D+I+GGM+ Y    G T    NPIF  G VEP +SEWL F GISVD +  R  +
Sbjct: 279 FIDMHVDLIKGGMETY----GVT---TNPIFMPGRVEPLYSEWLTFIGISVDHAENRNAY 338

Query: 62  LDATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEG 100
           +DAT+AY+ A LNAI+YL K+GY+ EQAYL+L   P EG
Sbjct: 339 MDATMAYRNACLNAIEYLKKWGYTGEQAYLILGTSPIEG 370

[Close]

BLAST of Cucsat.G2253 vs. NCBI nr
Match: XP_004141873.1 (uncharacterized protein LOC101206142 isoform X2 [Cucumis sativus])

HSP 1 Score: 255 bits (651), Expect = 1.58e-80
Identity = 127/148 (85.81%), Postives = 130/148 (87.84%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 410

Query: 122 DQ--------VPTFLRLLVWVGLISSSY 141
           DQ        VPT  RLL    ++  SY
Sbjct: 411 DQDIRPKPSKVPTGPRLLRRPDVLKCSY 438

[Close]

BLAST of Cucsat.G2253 vs. NCBI nr
Match: XP_011657890.1 (uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hypothetical protein Csa_003261 [Cucumis sativus])

HSP 1 Score: 255 bits (651), Expect = 1.66e-80
Identity = 127/148 (85.81%), Postives = 130/148 (87.84%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 412

Query: 122 DQ--------VPTFLRLLVWVGLISSSY 141
           DQ        VPT  RLL    ++  SY
Sbjct: 413 DQDIRPKPSKVPTGPRLLRRPDVLKCSY 440

[Close]

BLAST of Cucsat.G2253 vs. NCBI nr
Match: XP_008440368.1 (PREDICTED: formamidase-like isoform X3 [Cucumis melo])

HSP 1 Score: 252 bits (643), Expect = 2.52e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 410

Query: 122 DQ 123
           DQ
Sbjct: 411 DQ 412

[Close]

BLAST of Cucsat.G2253 vs. NCBI nr
Match: XP_008440367.1 (PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 252 bits (643), Expect = 2.66e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQ 123
           DQ
Sbjct: 413 DQ 414

[Close]

BLAST of Cucsat.G2253 vs. NCBI nr
Match: XP_008440366.1 (PREDICTED: formamidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 252 bits (643), Expect = 2.73e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 294 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 353

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 354 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 413

Query: 122 DQ 123
           DQ
Sbjct: 414 DQ 415

[Close]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL
Match: A0A0A0KGH0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1)

HSP 1 Score: 255 bits (651), Expect = 8.06e-81
Identity = 127/148 (85.81%), Postives = 130/148 (87.84%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 412

Query: 122 DQ--------VPTFLRLLVWVGLISSSY 141
           DQ        VPT  RLL    ++  SY
Sbjct: 413 DQDIRPKPSKVPTGPRLLRRPDVLKCSY 440

[Close]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL
Match: A0A1S3B0Y4 (formamidase-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 1.22e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 291 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 410

Query: 122 DQ 123
           DQ
Sbjct: 411 DQ 412

[Close]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL
Match: A0A5D3CLP5 (Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004150 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 1.29e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQ 123
           DQ
Sbjct: 413 DQ 414

[Close]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL
Match: A0A1S3B102 (formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 1.29e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 293 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 352

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 353 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 412

Query: 122 DQ 123
           DQ
Sbjct: 413 DQ 414

[Close]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL
Match: A0A1S3B0J2 (formamidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 1.32e-79
Identity = 118/122 (96.72%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +CDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL
Sbjct: 294 FLELKCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 353

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVD+PNAMVTLAIPTAIF
Sbjct: 354 DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDSPNAMVTLAIPTAIF 413

Query: 122 DQ 123
           DQ
Sbjct: 414 DQ 415

[Close]

BLAST of Cucsat.G2253 vs. TAIR 10
Match: AT4G37550.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 5.6e-62
Identity = 108/122 (88.52%), Postives = 116/122 (95.08%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVD+PNA+ TLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIF 410

Query: 122 DQ 124
           DQ
Sbjct: 411 DQ 412

[Close]

BLAST of Cucsat.G2253 vs. TAIR 10
Match: AT4G37550.3 (Acetamidase/Formamidase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 5.6e-62
Identity = 108/122 (88.52%), Postives = 116/122 (95.08%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 271 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 330

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGR+SGIVD+PNA+ TLAIPTAIF
Sbjct: 331 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRLSGIVDSPNAVATLAIPTAIF 390

Query: 122 DQ 124
           DQ
Sbjct: 391 DQ 392

[Close]

BLAST of Cucsat.G2253 vs. TAIR 10
Match: AT4G37560.1 (Acetamidase/Formamidase family protein )

HSP 1 Score: 234.2 bits (596), Expect = 7.3e-62
Identity = 115/148 (77.70%), Postives = 126/148 (85.14%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPT LHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTTLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQAYLLLSCCPCEGRISGIVDTPNAMVTLAIPTAIF 121
           DATVAYKRAVLNAIDYL KFGYSKEQ YLLLSCCPCEGRISGIVD+PNA+ TLAIPTAIF
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQVYLLLSCCPCEGRISGIVDSPNAVATLAIPTAIF 410

Query: 122 DQ--------VPTFLRLLVWVGLISSSY 142
           DQ        VPT  R++    ++ S+Y
Sbjct: 411 DQDIRPKTRKVPTGARIVKKPDVMKSTY 438

[Close]

BLAST of Cucsat.G2253 vs. TAIR 10
Match: AT4G37550.2 (Acetamidase/Formamidase family protein )

HSP 1 Score: 168.3 bits (425), Expect = 4.9e-42
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 2   FLFFRCDIIRGGMKEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGRQHFL 61
           FL  +C+IIR GM+EYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESG+QH+L
Sbjct: 291 FLELKCEIIRNGMQEYLTPMGPTPLHVNPIFEIGPVEPRFSEWLVFEGISVDESGKQHYL 350

Query: 62  DATVAYKRAVLNAIDYLHKFGYSKEQ 88
           DATVAYKRAVLNAIDYL KFGYSKEQ
Sbjct: 351 DATVAYKRAVLNAIDYLFKFGYSKEQ 376

The following BLAST results are available for this feature:

BLAST of Cucsat.G2253 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50228	1.8e-28	49.59	Formamidase OS=Methylophilus methylotrophus OX=17 GN=fmdA PE=1 SV=1	[more]
Q9URY7	2.6e-24	43.70	Putative formamidase C869.04 OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]
Q07838	6.7e-20	52.53	Acetamidase OS=Mycolicibacterium smegmatis OX=1772 GN=amdA PE=1 SV=2	[more]

BLAST of Cucsat.G2253 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141873.1	1.58e-80	85.81	uncharacterized protein LOC101206142 isoform X2 [Cucumis sativus]	[more]
XP_011657890.1	1.66e-80	85.81	uncharacterized protein LOC101206142 isoform X1 [Cucumis sativus] >KGN48598.1 hy...	[more]
XP_008440368.1	2.52e-79	96.72	PREDICTED: formamidase-like isoform X3 [Cucumis melo]	[more]
XP_008440367.1	2.66e-79	96.72	PREDICTED: formamidase-like isoform X2 [Cucumis melo] >TYK12823.1 formamidase-li...	[more]
XP_008440366.1	2.73e-79	96.72	PREDICTED: formamidase-like isoform X1 [Cucumis melo]	[more]

BLAST of Cucsat.G2253 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGH0	8.06e-81	85.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G495030 PE=4 SV=1	[more]
A0A1S3B0Y4	1.22e-79	96.72	formamidase-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]
A0A5D3CLP5	1.29e-79	96.72	Formamidase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3B102	1.29e-79	96.72	formamidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]
A0A1S3B0J2	1.32e-79	96.72	formamidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484841 PE=4 SV=1	[more]

BLAST of Cucsat.G2253 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37550.1	5.6e-62	88.52	Acetamidase/Formamidase family protein	[more]
AT4G37550.3	5.6e-62	88.52	Acetamidase/Formamidase family protein	[more]
AT4G37560.1	7.3e-62	77.70	Acetamidase/Formamidase family protein	[more]
AT4G37550.2	4.9e-42	89.53	Acetamidase/Formamidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004304	Acetamidase/Formamidase	PFAM	PF03069	FmdA_AmdA	coord: 12..295 e-value: 6.2E-113 score: 377.4
IPR004304	Acetamidase/Formamidase	PANTHER	PTHR31891	FORMAMIDASE C869.04-RELATED	coord: 1..295
None	No IPR available	GENE3D	2.60.120.580		coord: 150..295 e-value: 9.1E-20 score: 72.7
None	No IPR available	GENE3D	2.60.120.580		coord: 21..140 e-value: 1.0E-18 score: 69.2
None	No IPR available	PANTHER	PTHR31891:SF8	FORMAMIDASE-RELATED	coord: 1..295
None	No IPR available	SUPERFAMILY	141130	Acetamidase/Formamidase-like	coord: 25..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G2253.T11	Cucsat.G2253.T11	mRNA
Cucsat.G2253.T13	Cucsat.G2253.T13	mRNA
Cucsat.G2253.T16	Cucsat.G2253.T16	mRNA
Cucsat.G2253.T9	Cucsat.G2253.T9	mRNA
Cucsat.G2253.T2	Cucsat.G2253.T2	mRNA
Cucsat.G2253.T12	Cucsat.G2253.T12	mRNA
Cucsat.G2253.T14	Cucsat.G2253.T14	mRNA
Cucsat.G2253.T1	Cucsat.G2253.T1	mRNA
Cucsat.G2253.T3	Cucsat.G2253.T3	mRNA
Cucsat.G2253.T4	Cucsat.G2253.T4	mRNA
Cucsat.G2253.T7	Cucsat.G2253.T7	mRNA
Cucsat.G2253.T6	Cucsat.G2253.T6	mRNA
Cucsat.G2253.T10	Cucsat.G2253.T10	mRNA
Cucsat.G2253.T15	Cucsat.G2253.T15	mRNA
Cucsat.G2253.T8	Cucsat.G2253.T8	mRNA
Cucsat.G2253.T5	Cucsat.G2253.T5	mRNA
Cucsat.G2253.T19	Cucsat.G2253.T19	mRNA
Cucsat.G2253.T17	Cucsat.G2253.T17	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides