Cucsat.G21374.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G21374.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionprotein HAPLESS 2
Locationctg910: 1989181 .. 1993309 (+)
RNA-Seq ExpressionCucsat.G21374.T1
SyntenyCucsat.G21374.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAAGTGGAAGAAAACTCCACAAACAAGATGCAAACTCTGAGAACACCCCCGGTTTTGACTGTCAGCAAATCACCCGCTTATGTTTTGTATGAGCTAACATACATTCGTGTAATTCCCGCTTATGATTCTGTTTCCTTGTATCTAATTTTAATAATATGTTTGTGGTTACTGTGGTGTGGTGAAGTTCTAATCTGAATGTTTCATGTTTATGAAGTTTGATACTTGTATATATTTTTTATGTTTGAACACAATATGCTCAGGATGTTCCATATAAGCCTGAAGAATTTTATGTTCCAACTCGCAAATGCGAGCCGGATGCTAGCGCAAGAGTGGTACAAATATGTGAGAGGTAAGATAAGCCAGTCTTTTTAACTTGTTTTCCACGCAACCCCAAAGGTATTTGATTTACATTATTTTGTTTGATGTTTTCTTGTAGGTTAAGAGATGAAAGTGGACATATAATTCTGAGCACTCAAGTACCAATTTGAGAAAAGCTTCTATTGTCTTTGACTTTCTTTGGTTATATTGATTGATCTTCAGTCTTCACGTTCATTTTGATCTTGTTATTGATTCATTCTTTTTTGTTTCTTGTTAAATCTGTTTAGCCAATATGTTGTCCTTGTGGGGCAAAGCGTCGAATGCCTACGTCGTGTGGGAACTTTTGTAAGTTCGTTTTCCGTTTCTCCGGTTGCTAGTTCCTTCAAACACTGATTTGTGATCTTAACTCTTTTATTTCTAAGTCGACAAGATGATTAAAGGAAAGGCAAACACTGCACATTGTCTACGTTTTCCCGGTGACTGGTATGACTTATGTTTTCAGCTTTGTTTTTTGTCATTTTTTCCTTTTAATACTGATATATAGTAGTACTAGCAACCCCTTCTAAGAACATAACCTTATCATGAGGTCTTGATATTTATACTTTACCACCTTGATCTTAAACCGGTTTAGAAGCTTAACCATCATTATTATTATTCTTTTATTTACATTAAAGAGTGGATGTTTGAACCTCAAACTAAAATCCATTCAGTTAAGTTCTTGATAATTAACTTGTCATTCCAAAAAGCATACTTTTGGAGTTGCATTAATCAACTCATGTGTTACTTTAATGTATGTTGATATATTTAAGTATGTATCATATACATTAATGTAGGATGGTTGGATGAATTTGAAGTTGATGAAACTCAGCTTAGAACCTCCTTAAAGTGTACTACTAGCAAAAGATTCTAACTTAGAGTTTCTATCTCACTTAGGTTTCATGTTTTTAGTATTGGACAATGGACATTGGGATTCAGTGTTCAGATTCATGTGAAGTCAGGATCTAAAGTTTCGGTATGTTTTCCTCGATACCCAATGGAGAAAACTTCAATATTATCACTGTCTCATTCTCAAGTTTTGATCAAAATTTAGTGATTGACATTAAATTTAATTTTTTAGGAAGTGTCTGTGGGTCCTGAAAATAGAACAGTGGTGTCAAATGATAACTTCTTACGGGCTAATCTTATTGGTGACCTTGTTGGATACACAAATATTCCATCATTTGAGGACTTTTACCTTGTTATTCCCAGGCAGGTATATGCTTATTCCACCACAAAAGCATTTGATGGAACATTTTGTAATCGAAGATTTTTAGACATTAATGCGTACAACGGAAAGGCTACCTTGATATATATAACTTTGCTTGTTTGTTCTTATCTTCTGTTGCTAAGCTTAAAATATTCAATTGTGATGGAAGGGTGGTCCTGGTCAACCACAGAATTTAGGTACCAATTTCTCTATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGTAAGCTTTTAATCAAGAAAGGGTACTACTAACATTGATTGCTTTTATATGATCTGATACGTTTTCCCAATTTTCGAAAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAGTTAAGTATTGGAGGATATAGAATTATTCTTCATTCATGAGTATTCATATTGCTAAACGTTTTGTGTTGGGTGGGTTGAAGAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGTGAGTTGTTAGCACTTCTTGTAATTAAACTGCATCCCGAATGTGACTTGGTATGAATACGCTACGATACATGTTTAAGTGTCATTGTGCTAACAAGTGACCAATATATGTCCAACAAGCGTCGAAGTGTCTAAGTGCTTGCCAAACTTAAGTGTCCGTGTTTTGAACCAAATATATATATTTTTTTGAAGATATGAAAAGCGATTAGCAATCACATATGTTCTTCAATAGGCTACATTTTATTTAATGTAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGGTATTTACTAAGCTTTTACTACAATGAGATATATGGTTGGAATACTTGAGTGAAGCTTTTATTGTTTCTTTTGATCTCCTGCACATTATTTCAACTGGATGGATTTTTCTTTCTTGCAGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGTACCGTCTTTCGTGGAGACATTTTGATAGAATTTTGTTCATGTGATGAGTTTTTGATGAAAATCTAGATGATTTCAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGGTAAGATGAGTAACTAATTTGTGCTTCTTTTCTCATTAAATTTATTTAGTTCAGATTCAAGATTCTTTGAGACAAAACTTCTCTTTGTTGATAGTGCAGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGGTTCCTTTTACAGTTCTAGAATCATGGTGAATCAAGTATCCTTCTTCCATTAGTGAGTTTGGCTTTTTTTTTTTTTTTTTAACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGGTATTTGCTATCAAACTCTCATAAACCATTTACCTTCCATTGACATACCATCCCCAATGACACGTTTGCCTCTTTTGAACAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGAGTTGGCTTGTGTTGTTTGGTCTCTTTTTGGCCACTTTCCCCGCAGGTAACTATTATCCAACACTTGAAAAGTGATTTCAAACAAGTCGGCCTAAAATTAAAAATAAGCATCTCATCTTCATAATTTGTTTCAATTACTATGCAGTACTTGTGATACTGTGGGTTTTACATCAGAAAGGCTTATTTGACCCTCTTTATGACTGGTGGGAGGATATGTTTTGTCACAAAAGTGAGCCCACAAGGTCCACTTGGAAGTATAGAGGTGAAAGAAAACATTACCATAGACATGGTAGTAGGCATCACCAAAATCATGGGAGTGGATACAAGAGAAGAAGCCATGAATTACACAAAAAGCACAAACATTCTGAGAGAGACACAGATTACTTTCTTCACCATGTGCACAGGAAAAAAGGTAAGAGGGGACACAATAGAGTGTAGAATGTTTGATACAGGACAAATACTTGTCTTATTATAATTAATCATAGTATATACTTAAGTGTTCAATACAATATATACTATATTGAATATTGATTTTCAATATTACATGTAGAATAAGGAGTTGAGATTGATAGGAGTGGTAATATTTGAAGTTCCAGTCTACATCATTTCTGCTATAATCTTGTAGATTTTATCAGT

Coding sequence (CDS)

GAAGTGGAAGAAAACTCCACAAACAAGATGCAAACTCTGAGAACACCCCCGGTTTTGACTGTCAGCAAATCACCCGCTTATGTTTTGTATGAGCTAACATACATTCGTGATGTTCCATATAAGCCTGAAGAATTTTATGTTCCAACTCGCAAATGCGAGCCGGATGCTAGCGCAAGAGTGGTACAAATATGTGAGAGGTTAAGAGATGAAAGTGGACATATAATTCTGAGCACTCAACCAATATGTTGTCCTTGTGGGGCAAAGCGTCGAATGCCTACGTCGTGTGGGAACTTTTTCGACAAGATGATTAAAGGAAAGGCAAACACTGCACATTGTCTACGTTTTCCCGGTGACTGGTTTCATGTTTTTAGTATTGGACAATGGACATTGGGATTCAGTGTTCAGATTCATGTGAAGTCAGGATCTAAAGTTTCGGAAGTGTCTGTGGGTCCTGAAAATAGAACAGTGGTGTCAAATGATAACTTCTTACGGGCTAATCTTATTGGTGACCTTGTTGGATACACAAATATTCCATCATTTGAGGACTTTTACCTTGTTATTCCCAGGCAGGGTGGTCCTGGTCAACCACAGAATTTAGGTACCAATTTCTCTATGTGGATGCTACTAGAAAGAGTGAGATTTACTTTAGATGGTCTTGAATGCAACAAAATTGGTGTTGGTTATGAGACTTTTAATGGACAGCCTGATTTCTGTACATCCCCATTTTGGAGTTGTTTGCACAACCAATTATGGAATTTCAGGGAGGCCGATTTGAGTCGAATTGGTAGGAAACAATTGCCATTATATGGAGTGGAAGGACGGTTTGAGAGGATTAATCAACATCCAAATGCTGGGACACATTCGTTCTCCATTGGAGTCACTGAAGTTCTCAATACAAATCTTGTAATAGAATTGCGTGCTGATGATGTTGAGTATGTTTACCAAAGGAGTCCTGGGAAAATTATGAGCATCAGCATCCCAACTTTTGAAGCCCTCACACAATTTGGAGTTGCTACAGTTGCAACTAAGAACACTGGAGAAGTGGAAGCATCTTATAGCTTAACGTTTACTTGTTCAAAAGAAGTCAGTCTCATGGAGGAACAATATTATATCATGAAGCCAAATGAAATTGCTAGCCGTTCATTTAAACTCTACCCAACTACTGATCAAGCAGCAAAATATGTTTGTGCTGCCATACTTAAGGATGCTGATTTTAGTGAAGTTGATAGAGCTGAATGCCAATTTGCTACTACTGCTACTGTCCTTGACAATGGATCACAGATTACTCCTTTTGAACTCCCCAAGAAAAAGGAAAATGGTTTCATCCATTCAATCAAGCTCGCCTGGAAGCAGTTTTGGGGAAGCGTCATTGACTTTGTCACTGGGAAATCTTGCAGAAAAGTGTGCTCTGGATTTTTTGACTTCAGCTGTCACATACAATATATATGTTTGAGTTGGCTTGTGTTGTTTGGTCTCTTTTTGGCCACTTTCCCCGCAGTACTTGTGATACTGTGGGTTTTACATCAGAAAGGCTTATTTGACCCTCTTTATGACTGGTGGGAGGATATGTTTTGTCACAAAAGTGAGCCCACAAGGTCCACTTGGAAGTATAGAGGTGAAAGAAAACATTACCATAGACATGGTAGTAGGCATCACCAAAATCATGGGAGTGGATACAAGAGAAGAAGCCATGAATTACACAAAAAGCACAAACATTCTGAGAGAGACACAGATTACTTTCTTCACCATGTGCACAGGAAAAAAGGTAAGAGGGGACACAATAGAGTGTAG

Protein sequence

EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARVVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWFHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSFEDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Homology
BLAST of Cucsat.G21374.T1 vs. ExPASy Swiss-Prot
Match: F4JP36 (Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 2.5e-245
Identity = 404/612 (66.01%), Postives = 487/612 (79.58%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVE+NS++ MQT+R PPV+TV+KS AY LY+LTYIRDVPYKP+E++V TRKCE DA   +
Sbjct: 74  EVEDNSSSNMQTVRIPPVITVNKSAAYALYDLTYIRDVPYKPQEYHVTTRKCEHDAGPDI 133

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDE G+++  TQPICCPCG +RRMP+SCG+ FDKMIKGKANTAHCLRFPGDWF
Sbjct: 134 VQICERLRDEKGNVLEQTQPICCPCGPQRRMPSSCGDIFDKMIKGKANTAHCLRFPGDWF 193

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVF IGQ +LGFSV++ +K+G++VSEV +GPENRT  +NDNFL+ NLIGD  GYT+IPSF
Sbjct: 194 HVFGIGQRSLGFSVRVELKTGTRVSEVIIGPENRTATANDNFLKVNLIGDFGGYTSIPSF 253

Query: 181 EDFYLVIPRQGG-PGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCT 240
           EDFYLVIPR+    GQP +LG N+SMWMLLERVRFTLDGLECNKIGVGYE FN QP+FC+
Sbjct: 254 EDFYLVIPREAAEAGQPGSLGANYSMWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCS 313

Query: 241 SPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNT 300
           SP+WSCLHNQLWNFRE+D++RI R QLPLYG+EGRFERINQHPNAG HSFSIGVTE LNT
Sbjct: 314 SPYWSCLHNQLWNFRESDINRIDRHQLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNT 373

Query: 301 NLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCS 360
           NL+IELRADD+EYV+QRSPGKI++I+IPTFEALTQFGVA V  KNTGEVEASYSLTF CS
Sbjct: 374 NLMIELRADDIEYVFQRSPGKIINIAIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCS 433

Query: 361 KEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTA 420
           K V+ +EEQ++I+KP  + +RSFKLYPT DQAAKY+C AILKD+ FSEVDRAECQF+TTA
Sbjct: 434 KGVAFVEEQFFIIKPKAVTTRSFKLYPTKDQAAKYICTAILKDSQFSEVDRAECQFSTTA 493

Query: 421 TVLDNGSQIT-PFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCH 480
           TVLDNG+Q+T PF++P+ +  GF  SI++ W +    ++DF+TG +CR  CS FFDFSCH
Sbjct: 494 TVLDNGTQVTNPFQIPETQPKGFFDSIRILWTKIINGLVDFITGDTCRNKCSSFFDFSCH 553

Query: 481 IQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYR 540
           IQY+CLSW+V+FGL LA FP   ++LW+LHQKGLFDP YDWWED F           +  
Sbjct: 554 IQYVCLSWMVMFGLLLALFPITCLLLWLLHQKGLFDPCYDWWEDHFDLDHHRRLLPSRAD 613

Query: 541 GERKHYHRHGSRHHQNHGSGYKRRSHELHKKH---------------KHSERDTDYFLHH 596
              +H+H H  RHH NH     RR+H+ HK H                HS+    + LH 
Sbjct: 614 VVNRHHHHHKHRHHHNH----HRRTHQRHKHHHGQDDDVLQKMMLERDHSDSHYYHQLHR 673

BLAST of Cucsat.G21374.T1 vs. ExPASy Swiss-Prot
Match: Q5W6B9 (Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 6.8e-190
Identity = 340/608 (55.92%), Postives = 422/608 (69.41%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYI-RDVPYKPEEFYVPTRKCEPDASAR 60
           E E ++ +  +++R PPV+TVSKS  Y LY LTY+ RDV Y+P+E YV T KCEP A A+
Sbjct: 77  EEENDTPSATKSIRDPPVITVSKSATYALYALTYLDRDVAYRPDEKYVKTHKCEPYAGAK 136

Query: 61  VVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDW 120
           VV  CERL DE G++I  T+PICCPCG   R+ + CG+ + K+ KGKANTAHC+RFPGDW
Sbjct: 137 VVGECERLWDEKGNVIKQTEPICCPCG-PHRVQSKCGDIWSKLTKGKANTAHCVRFPGDW 196

Query: 121 FHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPS 180
           FHVF IG W+L FS+++ VK GS V +V VGPEN+TVVS DNFLR  ++GD  GYT+IPS
Sbjct: 197 FHVFGIGAWSLRFSIRVQVKKGSSVWDVVVGPENKTVVSGDNFLRVKVVGDYTGYTSIPS 256

Query: 181 FEDFYLVIPRQG-GPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFC 240
           FED YLV PR+G G  QPQ+LG   S WM+L+RVRFTLDGLEC+KIGVGYE +  QP+FC
Sbjct: 257 FEDNYLVTPRKGTGSSQPQDLGNEHSKWMILDRVRFTLDGLECDKIGVGYEAYRNQPNFC 316

Query: 241 TSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLN 300
           ++P+ SCL NQLWNF E D  RI   QLPLY VEGRF+RINQHPNAG H+FS+GVTE LN
Sbjct: 317 SAPYGSCLGNQLWNFWEYDKRRIDNSQLPLYIVEGRFQRINQHPNAGAHTFSVGVTEDLN 376

Query: 301 TNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTC 360
           TNL+IEL ADD+EYVYQRSP KI+ I +PTFEAL+Q G+A V TKN G++E+SYSLTF C
Sbjct: 377 TNLLIELMADDIEYVYQRSPAKIIDIRVPTFEALSQVGIANVTTKNIGKLESSYSLTFKC 436

Query: 361 SKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATT 420
           S  +S +EEQ Y MKP+E+ +RSF+L  TTDQAA + C AILK +DFSE+DR   +F+T 
Sbjct: 437 SSGISPVEEQLYTMKPDEVIARSFELRSTTDQAAMHQCEAILKASDFSELDREGYRFSTA 496

Query: 421 ATVLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCH 480
           ATV +NG+QI P      K+ GF  SIK  W+    ++IDF+TG+ C   C   FDF CH
Sbjct: 497 ATVYNNGAQIGPTN--DHKKGGFWDSIKALWR----NLIDFLTGRLCWTKCPRLFDFGCH 556

Query: 481 IQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYR 540
           IQY+C+ W++L    L   PA +V LW+LHQ+GLFDPLYDWW           R   K R
Sbjct: 557 IQYVCIGWILL----LLLIPAAVVFLWLLHQEGLFDPLYDWWGLEPDDDYRARRRHQKGR 616

Query: 541 GERKHY-------HRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHH----VHRK 596
             R H+       H HG  HH  HG  ++RR H       H     D   HH     H  
Sbjct: 617 HHRHHHDHRHRHGHSHGDHHHHYHGGHHQRRRH-------HHPPAWDVEGHHHDRQQHSH 666

BLAST of Cucsat.G21374.T1 vs. ExPASy Swiss-Prot
Match: B9G4M9 (Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.1e-175
Identity = 328/585 (56.07%), Postives = 406/585 (69.40%), Query Frame = 0

Query: 1   EVEENSTNKMQ-TLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASAR 60
           EVEEN T   +  +R P ++T++KS  Y LY+LTY+RDV YKPEE +V TRKCEP+A A 
Sbjct: 82  EVEENGTEAGEMPIRDPLIITINKSEVYALYDLTYLRDVAYKPEEKFVKTRKCEPEAGAN 141

Query: 61  VVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDW 120
           VV+ CERLRDE G II  T+P+CCPCG  RR+P+SCGN  DK+ KGKANTAHCLRFP DW
Sbjct: 142 VVKSCERLRDEKGSIIEHTEPVCCPCGPHRRVPSSCGNILDKVAKGKANTAHCLRFPDDW 201

Query: 121 FHVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPS 180
           FHVF IG+ +L FS+++ VK GS  SEV VGPENRTVVS D+ LR NL+GD  GYT++PS
Sbjct: 202 FHVFDIGRRSLWFSIRVQVKKGSSESEVIVGPENRTVVSEDSSLRVNLVGDFAGYTSLPS 261

Query: 181 FEDFYLVIPRQG-GPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFC 240
            E+FYLV PR+G G GQ + LG +FS WMLLERV FTLDGLECNKIGVGYE F  QP+FC
Sbjct: 262 LENFYLVTPRKGVGGGQLEVLGDDFSRWMLLERVLFTLDGLECNKIGVGYEAFRSQPNFC 321

Query: 241 TSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLN 300
           +SP  SCL +QL  F E D +R+   Q P Y V G+FERINQ+PNAG H+FS+G+ EVLN
Sbjct: 322 SSPLDSCLGDQLSKFWEIDKNRVNNSQPPQYVVLGKFERINQYPNAGVHTFSVGIPEVLN 381

Query: 301 TNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTC 360
           TNL+IEL ADD+EYVYQRS GKI+SI+I +FEAL+Q G A V TKN G +EASYSLTF C
Sbjct: 382 TNLMIELSADDIEYVYQRSSGKIISINISSFEALSQVGSARVKTKNIGRLEASYSLTFDC 441

Query: 361 SKEVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATT 420
              ++ +EEQY+IMKP+E   R+F L  +TDQA+ Y C AILK +DFSE+DR E QF+TT
Sbjct: 442 LSGINPVEEQYFIMKPDEKLIRTFDLRSSTDQASNYTCQAILKASDFSELDRKESQFSTT 501

Query: 421 ATVLDNGSQITPFELPKKKEN-GFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSC 480
           ATVL+NG+QI   E   K    GF  +IK    + W  +I+F TG +C   C  F  F  
Sbjct: 502 ATVLNNGTQIGSSENHTKGGIWGFFEAIKAWCAKMWHMLINFFTGTTCSTRCWSFLKF-- 561

Query: 481 HIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKY 540
                     V+ GL L      + +LW+LH+KGLFDPLY WW+ +   +++      + 
Sbjct: 562 ----------VIHGLLL------VAVLWLLHRKGLFDPLYYWWDGVVGSEAQERA---RR 621

Query: 541 RGERKHYHRHGSRHHQNHGSGYKRRSHEL--HKKHK--HSERDTD 579
           R +R H HRH S HH  H    KR   EL  H++H   H   D D
Sbjct: 622 RHKRAHSHRH-SHHHDAH----KRHKTELAGHRRHHVLHIHDDDD 640

BLAST of Cucsat.G21374.T1 vs. ExPASy Swiss-Prot
Match: A4GRC6 (Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 1.2e-37
Identity = 133/490 (27.14%), Postives = 203/490 (41.43%), Query Frame = 0

Query: 13  LRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYV-PTRKCEPDASARVVQICERLRDES 72
           L  P  ++++KSP +  Y L Y+    +KP E  + P+ K   D        C     + 
Sbjct: 95  LAAPLRVSLTKSPLWASYPLQYLSSFNWKPLEVILRPSNKVCKDGDWEDSPTCGWF-SQG 154

Query: 73  GHIILSTQPICCPCGAKRRMPTSCGNF----------------FDKMIKGKANTAHCLRF 132
           G  +  +Q  CC C + +    + G+                  D +I  K  +AHCL F
Sbjct: 155 GVRVADSQGFCCECSSSQVWDDTFGSSKERTRANLDCDFWSDPLDILIGRKPVSAHCLTF 214

Query: 133 PGDWFHVFSIGQWTLGFSVQIHVKSGSKVSEVS--------------------------- 192
              W+  + +G  +L F + I V+  +  S  +                           
Sbjct: 215 DPQWYSGYELGAASLQFEIAITVEVPTAPSPTTATTSATPRTNNSSSANSTNSTNSPAPQ 274

Query: 193 --------------VGPENRTVVSNDNFLRANLIGDLVGYTNIPSFEDFYLVIPR----Q 252
                         +GP      S    L A L+GDL  YT +P+  +  L++P+     
Sbjct: 275 FLSPPAPSTREVLHLGPSVPLASSASRLLSAKLLGDLAMYTQLPAISNQVLMVPQPPAAA 334

Query: 253 GGPGQP--QNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTSPFWSCLHN 312
              G P    L TN S WMLL++   ++DGL C+K+G G+  F  QP  C     +CL  
Sbjct: 335 AATGSPLDATLATNRSAWMLLDKTMLSMDGLACDKVGTGFSAFRYQPSGCGRAPQACLSG 394

Query: 313 QLWNFREADLSRIGRKQLPLYGV---EGRFERINQHPNAGTHSFSIGVTEVLNTNLVIEL 372
           QL +  EADL+RI   ++PLY +    G  +   Q  + G  SF++ VT    + + + +
Sbjct: 395 QLKDLWEADLARIADGRVPLYMITRFTGGSDTTLQSFSGGPLSFALPVTSHSQSLVTLSV 454

Query: 373 RADDVEYVYQRSPGKIMSISI--------PTFEALTQFGVATVATKNTGEVEASYSLTFT 423
            AD V  V  RSPGKI   ++          FEA+   G   V   NTG +++ Y+LT +
Sbjct: 455 AADGVRLVTNRSPGKITGAAVCRFAGTSCGGFEAVAARGYIYVNITNTGRLDSDYTLTVS 514

BLAST of Cucsat.G21374.T1 vs. ExPASy Swiss-Prot
Match: A0A060A682 (Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 3.0e-36
Identity = 143/588 (24.32%), Postives = 244/588 (41.50%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTR--KCEPDASA 60
           ++ ++  NK   L+   +  V+KSP   L+ L Y++D   +P E  + T    C+ D   
Sbjct: 67  QLSDSGVNKQ--LQNSFIFEVTKSPVTALFPLIYLQDFNSQPLEQVIATTLFSCK-DGFY 126

Query: 61  RVVQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDK-------MIKGKANTAH 120
                C+   D  G  IL +Q  CC C     +    GN   +        +   + TAH
Sbjct: 127 DSSPTCKFQYDSKGQKILDSQGYCCYCSLSDIL--GMGNDLSRGKVCYALNLGAGSATAH 186

Query: 121 CLRFPGDWFHVFSIGQWTLGFSVQIHVKSGSKVSE------VSVGPENRTVVSNDNFLRA 180
           CL+F   W+  F I Q+ L F V I++ +    ++      + +   N T+ S+DN   +
Sbjct: 187 CLKFSPLWYSAFKIQQYQLYFEVNINIYTVDSQNQKNLKQTLKLSTSNPTMKSSDNSTIS 246

Query: 181 NLIGDLVGYTNIPSFEDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIG 240
            +IG             +YLV P    P     +    S WM +++  FTLDG +CNKIG
Sbjct: 247 KIIGTFTPTQPPADLSSYYLVKP--SFPATDPRVLQGISSWMFVDKTMFTLDGTQCNKIG 306

Query: 241 VGYETFNGQPDFCTSPFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAG 300
           V Y  F  Q   C+ P  SCL NQL N  ++DL  + + + P Y +E +    NQ    G
Sbjct: 307 VSYSGFRQQSSSCSQPVGSCLQNQLENLYQSDLILLSQNKQPKYLLESQ-GNFNQVQFQG 366

Query: 301 THSFSIGVTEVLNTNLVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNT 360
                 G++   +T + IE+ A  +++V     G I   SI  FE+ +  G      +N 
Sbjct: 367 QTILQQGLSGSASTLITIEIDAAQIKFVTNLGIGCISQCSINNFESHSGNGKLVALVQNQ 426

Query: 361 GEVEASYSLTFTCSKEVSLMEEQYYIMKPNEIASRSFKLYPTTD-QAAKYVCAAILKDAD 420
           G   A + L F CS  V  ++ Q   +  N++ + +  +   +D  A    C   L DA 
Sbjct: 427 GNYSAEFVLGFNCSSNVQPIQGQKLFLTANQLYNFNCSVSVNSDISAINNNCTINLYDAI 486

Query: 421 FSEVDRAECQFATTATVLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKS 480
            +++D     F TT+T   +       +    +E                    + + +S
Sbjct: 487 GNQLDSKNILFNTTSTNHTSNQGNNTGQQQSSQE--------------------YKSSQS 546

Query: 481 CRKVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMF 540
           C   CS F+ F C+    C+         +A   + L ++  L + G   P+  +     
Sbjct: 547 CSDKCSSFWSFWCYFSAGCIKEAFKSIASIAGVASALALVIFLAKNGYLVPIIRFLCCCC 606

Query: 541 CHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKH 573
           C   +      K + ++K      S + ++  S    +S+++  K+K+
Sbjct: 607 CKSKKKENEKNKDKTDKKSIQESCS-YDRSCCSHSISQSYQVENKNKY 625

BLAST of Cucsat.G21374.T1 vs. NCBI nr
Match: XP_004143990.1 (protein HAPLESS 2 [Cucumis sativus])

HSP 1 Score: 1233 bits (3190), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ
Sbjct: 492 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 596
           RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Sbjct: 612 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 667

BLAST of Cucsat.G21374.T1 vs. NCBI nr
Match: XP_008437218.1 (PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo])

HSP 1 Score: 1182 bits (3058), Expect = 0.0
Identity = 573/591 (96.95%), Postives = 578/591 (97.80%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQ
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKR 591
           RKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 612 RKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKVKR 662

BLAST of Cucsat.G21374.T1 vs. NCBI nr
Match: KAA0042793.1 (protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1180 bits (3053), Expect = 0.0
Identity = 578/624 (92.63%), Postives = 584/624 (93.59%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCR-------------- 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCR              
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRYLLSKTIYLPLTYR 551

Query: 481 --------------KVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGL 540
                         K CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVILWVLHQKGL
Sbjct: 552 PLYFQMTCFSLLNRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVILWVLHQKGL 611

Query: 541 FDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKH 596
           FDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSHELHKKHKH
Sbjct: 612 FDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSHELHKKHKH 671

BLAST of Cucsat.G21374.T1 vs. NCBI nr
Match: XP_008437219.1 (PREDICTED: protein HAPLESS 2 isoform X2 [Cucumis melo])

HSP 1 Score: 1152 bits (2980), Expect = 0.0
Identity = 562/591 (95.09%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLT     
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLT----- 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
                 EQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 ------EQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQ
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKR 591
           RKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 612 RKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKVKR 651

BLAST of Cucsat.G21374.T1 vs. NCBI nr
Match: XP_038875720.1 (protein HAPLESS 2 [Benincasa hispida])

HSP 1 Score: 1143 bits (2956), Expect = 0.0
Identity = 548/596 (91.95%), Postives = 572/596 (95.97%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKS AYVLYELTYIRDVPYKPEEFYV TRKCEPDASA V
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSAAYVLYELTYIRDVPYKPEEFYVSTRKCEPDASASV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           V ICERLRD+SGHII ST+PICCPCGAKRRMPTSCGNFFDKMIKGK NTAHCLRFPGDWF
Sbjct: 132 VHICERLRDKSGHIIQSTEPICCPCGAKRRMPTSCGNFFDKMIKGKKNTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQW+LGFS+QIHVKSGSKVSEVS+GPENRT+VSNDNFLR NLIGDLVGYTNIPS 
Sbjct: 192 HVFSIGQWSLGFSIQIHVKSGSKVSEVSLGPENRTMVSNDNFLRVNLIGDLVGYTNIPSL 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQ GPG+PQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFN QPDFC S
Sbjct: 252 EDFYLVIPRQSGPGEPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCKS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PF SCLH+QLWN+READLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFRSCLHDQLWNYREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATV TKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVITKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQY+IMKP E+AS SFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYFIMKPKEVASLSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VLDNGSQITPF+LPKKKENGFI SIKL WK+ W S+++FVTGKSCRK CSGFFDFSCHIQ
Sbjct: 492 VLDNGSQITPFQLPKKKENGFIDSIKLIWKKLWESIVNFVTGKSCRKECSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFL TFPAVLVILWVLHQKGLFDPL+DWWED+FCHK+EPTRSTWK+RGE
Sbjct: 552 YICLSWLVLFGLFLTTFPAVLVILWVLHQKGLFDPLFDWWEDIFCHKNEPTRSTWKHRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 596
           RKH +RHGSRHHQNHGSGYKRRSHELHKKHKHS++DTDYFLHHVHRKKGKRGHNRV
Sbjct: 612 RKHSYRHGSRHHQNHGSGYKRRSHELHKKHKHSDKDTDYFLHHVHRKKGKRGHNRV 667

BLAST of Cucsat.G21374.T1 vs. ExPASy TrEMBL
Match: A0A0A0KQY8 (HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 PE=3 SV=1)

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 590/596 (98.99%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGD  
Sbjct: 132 VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGD-- 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
               IGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF
Sbjct: 192 ---CIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ
Sbjct: 492 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 596
           RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV
Sbjct: 612 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 662

BLAST of Cucsat.G21374.T1 vs. ExPASy TrEMBL
Match: A0A1S3ATM0 (protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 1182 bits (3058), Expect = 0.0
Identity = 573/591 (96.95%), Postives = 578/591 (97.80%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQ
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKR 591
           RKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 612 RKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKVKR 662

BLAST of Cucsat.G21374.T1 vs. ExPASy TrEMBL
Match: A0A5A7THM3 (Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002960 PE=3 SV=1)

HSP 1 Score: 1180 bits (3053), Expect = 0.0
Identity = 578/624 (92.63%), Postives = 584/624 (93.59%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCR-------------- 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCR              
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRYLLSKTIYLPLTYR 551

Query: 481 --------------KVCSGFFDFSCHIQYICLSWLVLFGLFLATFPAVLVILWVLHQKGL 540
                         K CSGFFDFSCHIQYICLSWLVLFGLFLATFP VLVILWVLHQKGL
Sbjct: 552 PLYFQMTCFSLLNRKECSGFFDFSCHIQYICLSWLVLFGLFLATFPIVLVILWVLHQKGL 611

Query: 541 FDPLYDWWEDMFCHKSEPTRSTWKYRGERKHYHRHGSRHHQNHGSGYKRRSHELHKKHKH 596
           FDPLYDWWED FCHKSE TRSTWKYRGERKH HRHGSRHH NHGSGYKRRSHELHKKHKH
Sbjct: 612 FDPLYDWWEDTFCHKSELTRSTWKYRGERKHNHRHGSRHHHNHGSGYKRRSHELHKKHKH 671

BLAST of Cucsat.G21374.T1 vs. ExPASy TrEMBL
Match: A0A1S3ATH0 (protein HAPLESS 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1)

HSP 1 Score: 1152 bits (2980), Expect = 0.0
Identity = 562/591 (95.09%), Postives = 567/591 (95.94%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKSPA+VLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSPAFVLYELTYIRDVPYKPEEFYVTTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           VQICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VQICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQNLG NFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNLGNNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR QLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVATVATKNTGEVEASYSLT     
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATVATKNTGEVEASYSLT----- 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
                 EQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT
Sbjct: 432 ------EQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VL+NGS+ITPF+LPKKKENGFI SIKLAWKQFWGSVIDFVTGKSCRK CSGFFDFSCHIQ
Sbjct: 492 VLENGSEITPFQLPKKKENGFIDSIKLAWKQFWGSVIDFVTGKSCRKECSGFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           YICLSWLVLFGLFLATFP VLVILWVLHQKGLFDPLYDWWED FCHKSE TRSTWKYRGE
Sbjct: 552 YICLSWLVLFGLFLATFPIVLVILWVLHQKGLFDPLYDWWEDTFCHKSELTRSTWKYRGE 611

Query: 541 RKHYHRHGSRHHQNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKGKR 591
           RKH HRHGSRHH NHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKK KR
Sbjct: 612 RKHNHRHGSRHHHNHGSGYKRRSHELHKKHKHSERDTDYFLHHVHRKKVKR 651

BLAST of Cucsat.G21374.T1 vs. ExPASy TrEMBL
Match: A0A6J1I226 (protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1)

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 541/599 (90.32%), Postives = 569/599 (94.99%), Query Frame = 0

Query: 1   EVEENSTNKMQTLRTPPVLTVSKSPAYVLYELTYIRDVPYKPEEFYVPTRKCEPDASARV 60
           EVEENSTNKMQTLRTPPVLTVSKS AYVLYELTYIRDVPYKPEEFYV TRKCEPDASARV
Sbjct: 72  EVEENSTNKMQTLRTPPVLTVSKSAAYVLYELTYIRDVPYKPEEFYVRTRKCEPDASARV 131

Query: 61  VQICERLRDESGHIILSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 120
           V ICERLRDESGHII STQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF
Sbjct: 132 VHICERLRDESGHIIQSTQPICCPCGAKRRMPTSCGNFFDKMIKGKANTAHCLRFPGDWF 191

Query: 121 HVFSIGQWTLGFSVQIHVKSGSKVSEVSVGPENRTVVSNDNFLRANLIGDLVGYTNIPSF 180
           HVFSIGQW+LGFSVQIHVKSGSK SEVSVGPENRTVVSNDNFLR NLIGDLVGYTNIPSF
Sbjct: 192 HVFSIGQWSLGFSVQIHVKSGSKASEVSVGPENRTVVSNDNFLRVNLIGDLVGYTNIPSF 251

Query: 181 EDFYLVIPRQGGPGQPQNLGTNFSMWMLLERVRFTLDGLECNKIGVGYETFNGQPDFCTS 240
           EDFYLVIPRQGGPGQPQN+G NFSMWMLLERVRFTLDGLECNKIGVGYETFN QPDFCTS
Sbjct: 252 EDFYLVIPRQGGPGQPQNMGHNFSMWMLLERVRFTLDGLECNKIGVGYETFNSQPDFCTS 311

Query: 241 PFWSCLHNQLWNFREADLSRIGRKQLPLYGVEGRFERINQHPNAGTHSFSIGVTEVLNTN 300
           PFWSCLHNQLWNFREADLSRIGR Q+PLYGVEGRFERINQHPNAGTHSFSIG+TEVLNTN
Sbjct: 312 PFWSCLHNQLWNFREADLSRIGRNQMPLYGVEGRFERINQHPNAGTHSFSIGITEVLNTN 371

Query: 301 LVIELRADDVEYVYQRSPGKIMSISIPTFEALTQFGVATVATKNTGEVEASYSLTFTCSK 360
           LVIELRADDVEYVYQRSPGKIMSI+IPTFEALTQFGVAT+ TKNTGE+EASYSLTFTCSK
Sbjct: 372 LVIELRADDVEYVYQRSPGKIMSINIPTFEALTQFGVATITTKNTGELEASYSLTFTCSK 431

Query: 361 EVSLMEEQYYIMKPNEIASRSFKLYPTTDQAAKYVCAAILKDADFSEVDRAECQFATTAT 420
           EVSLMEEQY++MKP E+ASRSFKLYPTTDQAAKYVC+AILKDADFSEVDRAECQFATT+T
Sbjct: 432 EVSLMEEQYFLMKPKEVASRSFKLYPTTDQAAKYVCSAILKDADFSEVDRAECQFATTST 491

Query: 421 VLDNGSQITPFELPKKKENGFIHSIKLAWKQFWGSVIDFVTGKSCRKVCSGFFDFSCHIQ 480
           VLDNGSQITPFELPKKKENGF+ SIKL WK+ WG+++DFV GKSCRK C+ FFDFSCHIQ
Sbjct: 492 VLDNGSQITPFELPKKKENGFVDSIKLVWKKLWGNIVDFVIGKSCRKECARFFDFSCHIQ 551

Query: 481 YICLSWLVLFGLFLATFPAVLVILWVLHQKGLFDPLYDWWEDMFCHKSEPTRSTWKYRGE 540
           Y+CLSWLVLFGLFLA FPAV+V++W+LHQKGLFDP+Y+WW DMF  KS PTR TWK+RGE
Sbjct: 552 YLCLSWLVLFGLFLAIFPAVVVVIWILHQKGLFDPVYEWWGDMFGAKSHPTRPTWKHRGE 611

Query: 541 RKHY--HRHGSRHHQNHGSGY-KRRSHELHKKHKHSERDTDYFLHHVHRKKGKRGHNRV 596
           RKH   HRHGSRH+QNHGSGY KRRSHE HKKHKHS+RDTDYFLHHVHRKK K GHNRV
Sbjct: 612 RKHSQSHRHGSRHYQNHGSGYHKRRSHESHKKHKHSDRDTDYFLHHVHRKKDKGGHNRV 670

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JP362.5e-24566.01Protein HAPLESS 2 OS=Arabidopsis thaliana OX=3702 GN=HAP2 PE=1 SV=1[more]
Q5W6B96.8e-19055.92Protein HAPLESS 2-A OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2A PE=2 SV=1[more]
B9G4M92.1e-17556.07Protein HAPLESS 2-B OS=Oryza sativa subsp. japonica OX=39947 GN=HAP2B PE=3 SV=1[more]
A4GRC61.2e-3727.14Hapless 2 OS=Chlamydomonas reinhardtii OX=3055 GN=HAP2 PE=1 SV=2[more]
A0A060A6823.0e-3624.32Hapless 2 OS=Tetrahymena thermophila OX=5911 GN=HAP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143990.10.0100.00protein HAPLESS 2 [Cucumis sativus][more]
XP_008437218.10.096.95PREDICTED: protein HAPLESS 2 isoform X1 [Cucumis melo][more]
KAA0042793.10.092.63protein HAPLESS 2 isoform X1 [Cucumis melo var. makuwa][more]
XP_008437219.10.095.09PREDICTED: protein HAPLESS 2 isoform X2 [Cucumis melo][more]
XP_038875720.10.091.95protein HAPLESS 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KQY80.098.99HAP2-GCS1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155520 P... [more]
A0A1S3ATM00.096.95protein HAPLESS 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A5A7THM30.092.63Protein HAPLESS 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3ATH00.095.09protein HAPLESS 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482714 PE=3 SV=1[more]
A0A6J1I2260.090.32protein HAPLESS 2 OS=Cucurbita maxima OX=3661 GN=LOC111468909 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018928Generative cell specific-1/HAP2 domainPFAMPF10699HAP2-GCS1coord: 2..483
e-value: 2.3E-120
score: 402.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..569
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..575
IPR040326HAP2/GCS1PANTHERPTHR31764PROTEIN HAPLESS 2coord: 1..589

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G21374Cucsat.G21374gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T1.E1Cucsat.G21374.T1.E1exon
Cucsat.G21374.T1.E2Cucsat.G21374.T1.E2exon
Cucsat.G21374.T1.E3Cucsat.G21374.T1.E3exon
Cucsat.G21374.T1.E4Cucsat.G21374.T1.E4exon
Cucsat.G21374.T1.E5Cucsat.G21374.T1.E5exon
Cucsat.G21374.T1.E6Cucsat.G21374.T1.E6exon
Cucsat.G21374.T1.E7Cucsat.G21374.T1.E7exon
Cucsat.G21374.T1.E8Cucsat.G21374.T1.E8exon
Cucsat.G21374.T1.E9Cucsat.G21374.T1.E9exon
Cucsat.G21374.T1.E10Cucsat.G21374.T1.E10exon
Cucsat.G21374.T1.E11Cucsat.G21374.T1.E11exon
Cucsat.G21374.T1.E12Cucsat.G21374.T1.E12exon
Cucsat.G21374.T1.E13Cucsat.G21374.T1.E13exon
Cucsat.G21374.T1.E14Cucsat.G21374.T1.E14exon
Cucsat.G21374.T1.E15Cucsat.G21374.T1.E15exon
Cucsat.G21374.T1.E16Cucsat.G21374.T1.E16exon
Cucsat.G21374.T1.E17Cucsat.G21374.T1.E17exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T1.C1Cucsat.G21374.T1.C1CDS
Cucsat.G21374.T1.C2Cucsat.G21374.T1.C2CDS
Cucsat.G21374.T1.C3Cucsat.G21374.T1.C3CDS
Cucsat.G21374.T1.C4Cucsat.G21374.T1.C4CDS
Cucsat.G21374.T1.C5Cucsat.G21374.T1.C5CDS
Cucsat.G21374.T1.C6Cucsat.G21374.T1.C6CDS
Cucsat.G21374.T1.C7Cucsat.G21374.T1.C7CDS
Cucsat.G21374.T1.C8Cucsat.G21374.T1.C8CDS
Cucsat.G21374.T1.C9Cucsat.G21374.T1.C9CDS
Cucsat.G21374.T1.C10Cucsat.G21374.T1.C10CDS
Cucsat.G21374.T1.C11Cucsat.G21374.T1.C11CDS
Cucsat.G21374.T1.C12Cucsat.G21374.T1.C12CDS
Cucsat.G21374.T1.C13Cucsat.G21374.T1.C13CDS
Cucsat.G21374.T1.C14Cucsat.G21374.T1.C14CDS
Cucsat.G21374.T1.C15Cucsat.G21374.T1.C15CDS
Cucsat.G21374.T1.C16Cucsat.G21374.T1.C16CDS
Cucsat.G21374.T1.C17Cucsat.G21374.T1.C17CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G21374.T1Cucsat.G21374.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061936 fusion of sperm to egg plasma membrane involved in double fertilization forming a zygote and endosperm
biological_process GO:0048235 pollen sperm cell differentiation
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0005886 plasma membrane