Cucsat.G21342.T7 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G21342.T7
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmeiotic recombination protein SPO11-2
Locationctg910: 1471471 .. 1475180 (+)
RNA-Seq ExpressionCucsat.G21342.T7
SyntenyCucsat.G21342.T7
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTCACAATTTTTGGATGAAAAACCGTGATTGCTTCACGGTTATTTACCATAGAGATGGTGATCTCTAAGGTGATAGAATGAAACGGGAGAGCCTCGCCAAGGCCAATGCCTTTTGCAACACTCTCCCAACTTTTGTTTGGAGGGAATATAACGATCACTATGGAATGATCGTTATATTTCCAACAACCAACATATATAATCAGCGCCATTTTCACCGGGTTTATGCTCGGTGACCTGCCGTCACAACTTCAACCAACTCCGCCTCTCCGATCCTTCAAACTCCTTCTGTAAGTTCACATCTGCTTTCTCTTGTTGTGTTGTCTTAATTGGAGTTATGATTCTTGGACTTTTGGTATGTACGTTCTTAGTTTATCGGAGTTTGTAGAAGTACAACTGAGTTTCGCATTTTTGGAAATAGTTTTTACATTGAAGCTAGATCATATCAAATTCTCCTCGTTATTGGATATATTACCCATCTTTAAATTGGTCTCATATCCGACACTTCCTGATGGGTGGCCATCCGTTGTTAATTTGCCATGGATTTAGTGAACATGTGAATGGAAGAGCTGCTGTCTGGATCAACTAAAAGGCTTTCGTTAAAAGTGTAACTGCCGTATAATGCAGCTTATTTTTACTAGTGCAGTTGCTAGGTGCAAGAAGTAAAGTAGAACAAGCAAATGAGTTAACTATTTGTCAATGTAATGAATAAGTTGAGATGATGATTTCCTGCAATTCGTCATCCAACCCACAATAGAACCCTTTCCCCGGCAGATTAGGGTAAGCATGAAGCAATTTCTTCATTACTCTGCTGATAAATAAATTTGTTGTTTAAAGCATTTTTTAGAAGAAAAAGGGTTTTGCTTAAATTAGATAGGTAATATGTCCAAAGCATTTTTTATACTAATGTGCAACGTCCTCTTTTAATTTTGTTATTTGTTTAAGAGGCTACAAAAAGAATACAAGAGCAACGAAATTCAAGTTGAATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATTCTCACTCCTCAAGAGGTATGATGAATTCACTTCATCATCCTTATCTCTAATCTTCGATTAGTGTTCCATTATATCTCAAGCATTGATTTCGAATAGGTCGTGGCTAGAATTCAAGTAGCTGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTCTGGTATGCTTCCTAACTAAGAAATGAAATGATATACGATAGATGAACAAAATTTGGATGCACTTAGACGGTATTACACGTTAATGATTGGACAGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACGAGGTCTTTGATGAGACCCAACGCCTCTAAGGCTTTTGTCAGAGGTATTGTAAATGGAATCTAATCTTCGATGGTCAGATTTTTTTAATGATCACTTTGTTAGCCTATTTTTTTGTCTACTTTTGAGCTTTCCTTATTTTGTGATGTAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTCTGATTCAGGATAAGAAAGTGACGCAGAGGGAGCTTTTCTATAAGCTGCTCTGTGATTCACCGGTTTATTTTTCGACTCAGTTGCATGTCAATAGGACAATTCAAGGTGATTTTGTTCAACGAAGACCTGCTTACTAGTTAAGAACTAAAACTATATTAAGCATAATGATCAAAGTGCAGTAGTTTTATTGCAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCCGGCCGTCTACTGTTGCAGGCACATTGCGCTGTTTTTCTTATCGGTTTGGTTATTTACGTTGCACATCACCACATCACATATATACGCATGAAGTGATAACCCGGCTTTTATTGACCCCTATTATTCTACTTTTGCATCACAACTACGTTGTGAATCCTTGAAATCAGGGTATTGATTTGATTATACATTGTTTATTGGTTTCTGGCCAACATGCACTTTTAAGGTATCTTCTACTAAACCAAATTCTTGGACGTTATGGCCTTCCTGAACAATTTTGATTCCTATAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGCAGACGTTGACTCTGAAGACTGACGCTCGTTACATAATTGTAATCGAAAAGGTCATTTTTCTTAAATTATGACCTGCTTTTTAATTATACTTTTGTGAAAACAATAATTCAAAAAATGCAGTCTATCATAATGTGTGCAAATGCATCAAACATTATTGGTTATGCAGCATGCGATATTCCAACGTTTGGCAGATGATCGCGTTTTCAATCGCATTCCTAGTATTCTTATCACAGCCAAAGGATATCCAGACTTAGCCACAAGGTAATTTTGTTCAACAGTTCATAAATCGCCATTCCTTTTGTTGCCTTCTTGGAAGTCGTAACTTAAACATATGAACTGCCTTCTCAGGTACTTTCTTCACAAAATTTGCAAAACGTTTTCGCATCTGCCAATGTTCGGCCTTGTTGATTGGTACGAGATCCTCCTTTACATCAACTCAACAAACTTCATAATTTTTTTCTCACTTTTAGCTTCTGGGTTCTAAAGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGGTAAGTCGCATTCATTTATTTGTTCGATCTGTTTCTAATTGCAAATCATAAAACTGAATAATCTCTTTACATTACCAGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGCTGTCCTCAAAAATATTACAGGTATCGTGCATAATTCTATACCTTTGATGCTATGGCATTTTAGGCTATCAAAATGCTTAAGAATATATCAAACCAACAGGAGAACTATCGACAAGAGCTGACATTAATGGTTGAGAGAGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTACTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGACATTATTTTCAACCTAGAGATGTAGAGGCATCTGCATAGAGTGAATAAAGGGCTATTTGCTAAAAAAACCATACCTTCTTTTTCAAGTCCATCTTTCTTAGAAGAGAAGTTCACAAAAACTGAAGAGACTCGTTCATCAATCAAAGTTTTCAGCTGCTTCAACTTTAAAGATTCCCCCGGTTCCTTTCCCCATGTTGCCATACTACGACGTCACCTGAGTCTCCATTGACATCAAAGGAAGTCTTTCTCCCTTGAAAGCTACTCTTAATATCCAACCCTTGCTGCCCCTAGTTGTGGATTTATCCTATAAAGTTCCAAAGTAGAAAAACTATTAGGTGCTATCTGTGAAAGAAACGGAACAGAAGATGGGAGGTTTTGAACAGCTAGCAAATGATGTAGTTTAAAGTTAAAATTGAAAGATGCATGACTAAAGGGGTTTTTAAGATATGAATGATTTATAATTCCTTTCGATGTGATCAAAGTCAAACGACTTGCTCATT

Coding sequence (CDS)

ATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATTCTCACTCCTCAAGAGGTCGTGGCTAGAATTCAAGTAGCTGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTCTGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACGAGGTCTTTGATGAGACCCAACGCCTCTAAGGCTTTTGTCAGAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTCTGATTCAGGATAAGAAAGTGACGCAGAGGGAGCTTTTCTATAAGCTGCTCTGTGATTCACCGGTTTATTTTTCGACTCAGTTGCATGTCAATAGGACAATTCAAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCCGGCCGTCTACTGTTGCAGGCACATTGCGCTGTTTTTCTTATCGGTTTGGTTATTTACGTTGCACATCACCACATCACATATATACGCATGAAGTGA

Protein sequence

MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCAVFLIGLVIYVAHHHITYIRMK
Homology
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BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match: Q9M4A1 (Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.9e-61
Identity = 124/175 (70.86%), Postives = 146/175 (83.43%), Query Frame = 0 

Query: 2   EPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDRR 61
           E +GL  SSM+FFSDQ L YADIL P E  ARI+V+VLN LRILNS  PAIS+L LI+R+
Sbjct: 3   ESSGL--SSMKFFSDQHLSYADILLPHEARARIEVSVLNLLRILNSPDPAISDLSLINRK 62

Query: 62  SSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQR 121
            SNS +++GILTD S+IFLS SF   SL     +KAFVRVWK+ME+C QIL+Q+K+VTQR
Sbjct: 63  RSNSCINKGILTDVSYIFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQR 122

Query: 122 ELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           ELFYKLLCDSP YFS+Q+ VNR++QDVVALL+CSRYSLGIMASSRGLVAGRL LQ
Sbjct: 123 ELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQ 175

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match: Q5ZPI9 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.3e-46
Identity = 104/178 (58.43%), Postives = 135/178 (75.84%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI+VAVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL+LQ
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVLQ 177

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match: Q6ZD95 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-2 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-45
Identity = 103/178 (57.87%), Postives = 135/178 (75.84%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI+VAVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL++Q
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVVQ 177

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 3.2e-06
Identity = 44/154 (28.57%), Postives = 79/154 (51.30%), Query Frame = 0 

Query: 24  ILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSH 83
           + +  EV+  I+  + + ++ L  +  PA +    ID RSS     + I+ DDS      
Sbjct: 41  VASSSEVLTAIENIIQDIIKSLARNEVPAFT----IDNRSS----WENIMFDDSVGLRMI 100

Query: 84  SFCTRSLMR---PNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQL 143
             CT   +R   P + K F  + K++ M  +++  D   T+R+++Y    DS + F  Q 
Sbjct: 101 PQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYY---TDSQL-FGNQA 160

Query: 144 HVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRL 174
            V+  I D+  +L+  R SL ++++S+GL+AG L
Sbjct: 161 AVDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNL 182

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.2e-06
Identity = 44/154 (28.57%), Postives = 82/154 (53.25%), Query Frame = 0 

Query: 24  ILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSH 83
           + +  EV+A I+  + + +  L  + +PA +    ID RSS     + I  +DS      
Sbjct: 41  LASSSEVLASIENIIQDIITSLARNEAPAFT----IDNRSS----WENIKFEDSVGLQMV 100

Query: 84  SFCTRSLMR---PNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQL 143
           S CT   ++   P +++ F  + K++ M  +++  +   T+R+++Y    DS + F  Q 
Sbjct: 101 SHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYY---TDSQL-FGNQT 160

Query: 144 HVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRL 174
            V+  I D+  +L+ SR SL I+++S+GL+AG L
Sbjct: 161 VVDNIINDISCMLKVSRRSLHILSTSKGLIAGNL 182

[Close]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match: KAA0042721.1 (meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa])

HSP 1 Score: 355 bits (912), Expect = 5.09e-120
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHC 
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 VFLIGL 186
            FLIGL
Sbjct: 181 AFLIGL 186

[Close]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match: XP_004143970.1 (meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654718.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_031741008.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >KGN50057.1 hypothetical protein Csa_000678 [Cucumis sativus])

HSP 1 Score: 342 bits (878), Expect = 5.31e-115
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match: XP_008437337.1 (PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437341.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437342.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo])

HSP 1 Score: 339 bits (870), Expect = 8.70e-114
Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match: XP_038906477.1 (meiotic recombination protein SPO11-2 isoform X2 [Benincasa hispida])

HSP 1 Score: 320 bits (819), Expect = 1.12e-107
Identity = 163/176 (92.61%), Postives = 169/176 (96.02%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEP GLF+SSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPTGLFKSSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRV+QGILTDD WIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVNQGILTDDLWIFLSHSFCTRSLMRSNAAKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALLQCSR SLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLQCSRCSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match: KAG7036015.1 (Meiotic recombination protein SPO11-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321 bits (822), Expect = 1.89e-106
Identity = 160/180 (88.89%), Postives = 174/180 (96.67%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           ME  GLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL+SS+PAISNLPLIDR
Sbjct: 1   METTGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILSSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
            SSNSRV+QGILTD+SWIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  SSSNSRVNQGILTDESWIFLSHSFCTRSLMRANAAKAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSR+SLG+MASSRGLVAGRLLLQAHC+
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRFSLGVMASSRGLVAGRLLLQAHCS 180

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match: A0A5A7TMD5 (Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002190 PE=3 SV=1)

HSP 1 Score: 355 bits (912), Expect = 2.47e-120
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHC 
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 VFLIGL 186
            FLIGL
Sbjct: 181 AFLIGL 186

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match: A0A0A0KNU2 (TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3 SV=1)

HSP 1 Score: 342 bits (878), Expect = 2.57e-115
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match: A0A1S3AUC6 (meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE=3 SV=1)

HSP 1 Score: 339 bits (870), Expect = 4.21e-114
Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match: A0A6J1DX60 (meiotic recombination protein SPO11-2 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 312 bits (799), Expect = 6.00e-105
Identity = 158/176 (89.77%), Postives = 170/176 (96.59%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPAGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNS V++GILTD SWI+LSHSFCTRSLMR NA++AFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  RSSNSGVNRGILTDVSWIYLSHSFCTRSLMRANAAEAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match: A0A6J1DSB8 (meiotic recombination protein SPO11-2 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 312 bits (799), Expect = 6.87e-105
Identity = 158/176 (89.77%), Postives = 170/176 (96.59%), Query Frame = 0 

Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPAGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNS V++GILTD SWI+LSHSFCTRSLMR NA++AFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  RSSNSGVNRGILTDVSWIYLSHSFCTRSLMRANAAEAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLLQ 176

The following BLAST results are available for this feature:
BLAST of Cucsat.G21342.T7 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M4A15.9e-6170.86Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2... [more]
Q5ZPI95.3e-4658.43Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q6ZD951.2e-4557.87Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9WTK83.2e-0628.57Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2[more]
Q9Y5K19.2e-0628.57Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1[more]
BLAST of Cucsat.G21342.T7 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0042721.15.09e-12097.31meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa][more]
XP_004143970.15.31e-115100.00meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic ... [more]
XP_008437337.18.70e-11498.30PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 ... [more]
XP_038906477.11.12e-10792.61meiotic recombination protein SPO11-2 isoform X2 [Benincasa hispida][more]
KAG7036015.11.89e-10688.89Meiotic recombination protein SPO11-2 [Cucurbita argyrosperma subsp. argyrosperm... [more]
BLAST of Cucsat.G21342.T7 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7TMD52.47e-12097.31Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0KNU22.57e-115100.00TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3... [more]
A0A1S3AUC64.21e-11498.30meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE... [more]
A0A6J1DX606.00e-10589.77meiotic recombination protein SPO11-2 isoform X4 OS=Momordica charantia OX=3673 ... [more]
A0A6J1DSB86.87e-10589.77meiotic recombination protein SPO11-2 isoform X3 OS=Momordica charantia OX=3673 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002815Spo11/DNA topoisomerase VI subunit APRINTSPR01550TOP6AFAMILYcoord: 156..171
score: 45.31
coord: 138..155
score: 27.78
IPR002815Spo11/DNA topoisomerase VI subunit APANTHERPTHR10848MEIOTIC RECOMBINATION PROTEIN SPO11coord: 16..177
IPR013049Spo11/DNA topoisomerase VI, subunit A, N-terminalPFAMPF04406TP6A_Ncoord: 96..161
e-value: 5.7E-16
score: 58.3
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..164
e-value: 7.1E-22
score: 79.8
NoneNo IPR availablePANTHERPTHR10848:SF0MEIOTIC RECOMBINATION PROTEIN SPO11coord: 16..177
IPR036078Spo11/DNA topoisomerase VI subunit A superfamilySUPERFAMILY56726DNA topoisomerase IV, alpha subunitcoord: 96..178

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G21342Cucsat.G21342gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21342.T7.E1Cucsat.G21342.T7.E1exon
Cucsat.G21342.T7.E2Cucsat.G21342.T7.E2exon
Cucsat.G21342.T7.E3Cucsat.G21342.T7.E3exon
Cucsat.G21342.T7.E4Cucsat.G21342.T7.E4exon
Cucsat.G21342.T7.E5Cucsat.G21342.T7.E5exon
Cucsat.G21342.T7.E6Cucsat.G21342.T7.E6exon
Cucsat.G21342.T7.E7Cucsat.G21342.T7.E7exon
Cucsat.G21342.T7.E8Cucsat.G21342.T7.E8exon
Cucsat.G21342.T7.E9Cucsat.G21342.T7.E9exon
Cucsat.G21342.T7.E10Cucsat.G21342.T7.E10exon
Cucsat.G21342.T7.E11Cucsat.G21342.T7.E11exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21342.T7.C1Cucsat.G21342.T7.C1CDS
Cucsat.G21342.T7.C2Cucsat.G21342.T7.C2CDS
Cucsat.G21342.T7.C3Cucsat.G21342.T7.C3CDS
Cucsat.G21342.T7.C4Cucsat.G21342.T7.C4CDS
Cucsat.G21342.T7.C5Cucsat.G21342.T7.C5CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G21342.T7Cucsat.G21342.T7-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0051026 chiasma assembly
biological_process GO:0009553 embryo sac development
biological_process GO:0000706 meiotic DNA double-strand break processing
biological_process GO:0009555 pollen development
biological_process GO:0048316 seed development
cellular_component GO:0005694 chromosome
cellular_component GO:0000228 nuclear chromosome
molecular_function GO:0005524 ATP binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding