Cucsat.G21342 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21342
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	meiotic recombination protein SPO11-2
Location	ctg910: 1471474 .. 1475162 (+)
RNA-Seq Expression	Cucsat.G21342
Synteny	Cucsat.G21342

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTCACTTTTAGTGTCACAATTTTTGGATGAAAAACCGTGATTGCTTCACGGTTATTTACCATAGAGATGGTGATCTCTAAGGTGATAGAATGAAACGGGAGAGCCTCGCCAAGGCCAATGCCTTTTGCAACACTCTCCCAACTTTTGTTTGGAGGGAATATAACGATCACTATGGAATGATCGTTATATTTCCAACAACCAACATATATAATCAGCGCCATTTTCACCGGGTTTATGCTCGGTGACCTGCCGTCACAACTTCAACCAACTCCGCCTCTCCGATCCTTCAAACTCCTTCTGTAAGTTCACATCTGCTTTCTCTTGTTGTGTTGTCTTAATTGGAGTTATGATTCTTGGACTTTTGGTATGTACGTTCTTAGTTTATCGGAGTTTGTAGAAGTACAACTGAGTTTCGCATTTTTGGAAATAGTTTTTACATTGAAGCTAGATCATATCAAATTCTCCTCGTTATTGGATATATTACCCATCTTTAAATTGGTCTCATATCCGACACTTCCTGATGGGTGGCCATCCGTTGTTAATTTGCCATGGATTTAGTGAACATGTGAATGGAAGAGCTGCTGTCTGGATCAACTAAAAGGCTTTCGTTAAAAGTGTAACTGCCGTATAATGCAGCTTATTTTTACTAGTGCAGTTGCTAGGTGCAAGAAGTAAAGTAGAACAAGCAAATGAGTTAACTATTTGTCAATGTAATGAATAAGTTGAGATGATGATTTCCTGCAATTCGTCATCCAACCCACAATAGAACCCTTTCCCCGGCAGATTAGGGTAAGCATGAAGCAATTTCTTCATTACTCTGCTGATAAATAAATTTGTTGTTTAAAGCATTTTTTAGAAGAAAAAGGGTTTTGCTTAAATTAGATAGGTAATATGTCCAAAGCATTTTTTATACTAATGTGCAACGTCCTCTTTTAATTTTGTTATTTGTTTAAGAGGCTACAAAAAGAATACAAGAGCAACGAAATTCAAGTTGAATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATTCTCACTCCTCAAGAGGTATGATGAATTCACTTCATCATCCTTATCTCTAATCTTCGATTAGTGTTCCATTATATCTCAAGCATTGATTTCGAATAGGTCGTGGCTAGAATTCAAGTAGCTGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTCTGGTATGCTTCCTAACTAAGAAATGAAATGATATACGATAGATGAACAAAATTTGGATGCACTTAGACGGTATTACACGTTAATGATTGGACAGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACGAGGTCTTTGATGAGACCCAACGCCTCTAAGGCTTTTGTCAGAGGTATTGTAAATGGAATCTAATCTTCGATGGTCAGATTTTTTTAATGATCACTTTGTTAGCCTATTTTTTTGTCTACTTTTGAGCTTTCCTTATTTTGTGATGTAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTCTGATTCAGGATAAGAAAGTGACGCAGAGGGAGCTTTTCTATAAGCTGCTCTGTGATTCACCGGTTTATTTTTCGACTCAGTTGCATGTCAATAGGACAATTCAAGGTGATTTTGTTCAACGAAGACCTGCTTACTAGTTAAGAACTAAAACTATATTAAGCATAATGATCAAAGTGCAGTAGTTTTATTGCAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCCGGCCGTCTACTGTTGCAGGCACATTGCGCTGTTTTTCTTATCGGTTTGGTTATTTACGTTGCACATCACCACATCACATATATACGCATGAAGTGATAACCCGGCTTTTATTGACCCCTATTATTCTACTTTTGCATCACAACTACGTTGTGAATCCTTGAAATCAGGGTATTGATTTGATTATACATTGTTTATTGGTTTCTGGCCAACATGCACTTTTAAGGTATCTTCTACTAAACCAAATTCTTGGACGTTATGGCCTTCCTGAACAATTTTGATTCCTATAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGCAGACGTTGACTCTGAAGACTGACGCTCGTTACATAATTGTAATCGAAAAGGTCATTTTTCTTAAATTATGACCTGCTTTTTAATTATACTTTTGTGAAAACAATAATTCAAAAAATGCAGTCTATCATAATGTGTGCAAATGCATCAAACATTATTGGTTATGCAGCATGCGATATTCCAACGTTTGGCAGATGATCGCGTTTTCAATCGCATTCCTAGTATTCTTATCACAGCCAAAGGATATCCAGACTTAGCCACAAGGTAATTTTGTTCAACAGTTCATAAATCGCCATTCCTTTTGTTGCCTTCTTGGAAGTCGTAACTTAAACATATGAACTGCCTTCTCAGGTACTTTCTTCACAAAATTTGCAAAACGTTTTCGCATCTGCCAATGTTCGGCCTTGTTGATTGGTACGAGATCCTCCTTTACATCAACTCAACAAACTTCATAATTTTTTTCTCACTTTTAGCTTCTGGGTTCTAAAGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGGTAAGTCGCATTCATTTATTTGTTCGATCTGTTTCTAATTGCAAATCATAAAACTGAATAATCTCTTTACATTACCAGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGCTGTCCTCAAAAATATTACAGGTATCGTGCATAATTCTATACCTTTGATGCTATGGCATTTTAGGCTATCAAAATGCTTAAGAATATATCAAACCAACAGGAGAACTATCGACAAGAGCTGACATTAATGGTTGAGAGAGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTACTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGACATTATTTTCAACCTAGAGATGTAGAGGCATCTGCATAGAGTGAATAAAGGGCTATTTGCTAAAAAAACCATACCTTCTTTTTCAAGTCCATCTTTCTTAGAAGAGAAGTTCACAAAAACTGAAGAGACTCGTTCATCAATCAAAGTTTTCAGCTGCTTCAACTTTAAAGATTCCCCCGGTTCCTTTCCCCATGTTGCCATACTACGACGTCACCTGAGTCTCCATTGACATCAAAGGAAGTCTTTCTCCCTTGAAAGCTACTCTTAATATCCAACCCTTGCTGCCCCTAGTTGTGGATTTATCCTATAAAGTTCCAAAGTAGAAAAACTATTAGGTGCTATCTGTGAAAGAAACGGAACAGAAGATGGGAGGTTTTGAACAGCTAGCAAATGATGTAGTTTAAAGTTAAAATTGAAAGATGCATGACTAAAGGGGTTTTTAAGATATGAATGATTTATAATTCCTTTCGATGTGATCA

Coding sequence (CDS)

ATGGAGCCGGCAGGTTTATTCGAATCCTCCATGAGATTTTTCTCTGATCAAGAACTCTGCTATGCTGATATTCTCACTCCTCAAGAGGTCGTGGCTAGAATTCAAGTAGCTGTCCTCAATTTTCTCAGAATTCTCAACTCCTCCTCTCCAGCCATCTCAAATCTTCCTCTGATTGATCGAAGATCGAGCAATAGCCGAGTGAGTCAAGGAATTTTGACAGATGATTCGTGGATATTCCTTTCGCATTCCTTTTGTACGAGGTCTTTGATGAGACCCAACGCCTCTAAGGCTTTTGTCAGAGTTTGGAAGCTGATGGAGATGTGCTCTCAGATTCTGATTCAGGATAAGAAAGTGACGCAGAGGGAGCTTTTCTATAAGCTGCTCTGTGATTCACCGGTTTATTTTTCGACTCAGTTGCATGTCAATAGGACAATTCAAGATGTTGTAGCCTTACTTCAATGTAGCCGTTACAGCCTTGGAATTATGGCATCTAGTAGGGGACTTGTCGCCGGCCGTCTACTGTTGCAGGAGCCTGAGCAAGAAGTTGTGGACTGCACTGCCTGTGGGTCTTCTGGATATCCCATTTCAGGTGACTTGGACTTGCTGCAGACGTTGACTCTGAAGACTGACGCTCGTTACATAATTGTAATCGAAAAGCATGCGATATTCCAACGTTTGGCAGATGATCGCGTTTTCAATCGCATTCCTAGTATTCTTATCACAGCCAAAGGATATCCAGACTTAGCCACAAGGTACTTTCTTCACAAAATTTGCAAAACGTTTTCGCATCTGCCAATGTTCGGCCTTGTTGATTGGAACCCTGCGGGATTGGCCATTTTATGCACCTTCAAATACGGAAGTATAGGGATGGGCTTGGAGGCATACAGATATGCTTGCAATGTTAAATGGTTGGGAGTACGAGGAGATGATTTACAACTGATACCACAAGAATCTCTGGTTCCACTCAAGCCCCGGGACCTACAAATTGCCAAAAGCTTGCTGTCCTCAAAAATATTACAGGAGAACTATCGACAAGAGCTGACATTAATGGTTGAGAGAGGGCAAAAGGCTGAACTTGAAGCTTTGTACCACAATGGATTCGATTACTTGGAGAAGTACATAGTCAAAAAGATTATTCAATTTAGTTACATCTGA

Protein sequence

MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQEPEQEVVDCTACGSSGYPISGDLDLLQTLTLKTDARYIIVIEKHAIFQRLADDRVFNRIPSILITAKGYPDLATRYFLHKICKTFSHLPMFGLVDWNPAGLAILCTFKYGSIGMGLEAYRYACNVKWLGVRGDDLQLIPQESLVPLKPRDLQIAKSLLSSKILQENYRQELTLMVERGQKAELEALYHNGFDYLEKYIVKKIIQFSYI

Homology

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BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot
Match: Q9M4A1 (Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.9e-61
Identity = 124/175 (70.86%), Postives = 146/175 (83.43%), Query Frame = 0


Query: 2   EPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDRR 61
           E +GL  SSM+FFSDQ L YADIL P E  ARI+V+VLN LRILNS  PAIS+L LI+R+
Sbjct: 3   ESSGL--SSMKFFSDQHLSYADILLPHEARARIEVSVLNLLRILNSPDPAISDLSLINRK 62

Query: 62  SSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQR 121
            SNS +++GILTD S+IFLS SF   SL     +KAFVRVWK+ME+C QIL+Q+K+VTQR
Sbjct: 63  RSNSCINKGILTDVSYIFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQR 122

Query: 122 ELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           ELFYKLLCDSP YFS+Q+ VNR++QDVVALL+CSRYSLGIMASSRGLVAGRL LQ
Sbjct: 123 ELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQ 175

[Close]

BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot
Match: Q5ZPI9 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-2 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.3e-46
Identity = 104/178 (58.43%), Postives = 135/178 (75.84%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI+VAVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL+LQ
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVLQ 177

[Close]

BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot
Match: Q6ZD95 (Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-2 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-45
Identity = 103/178 (57.87%), Postives = 135/178 (75.84%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLID 60
           M  AG+  +S+ F +D+ LC ADIL P EV ARI+VAVLNFL  L + ++PAIS LPLI 
Sbjct: 1   MAEAGVAAASL-FGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLIS 60

Query: 61  RRSSNSRVSQGILTDD-SWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKV 120
           R ++N  + + +L DD S ++LS++ C RSL R N +KAFVRVWK+MEMC +IL + K V
Sbjct: 61  RGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLV 120

Query: 121 TQRELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 177
           T RELFY LL +SP YF+ Q HVN+T+QDVV+LL+C+R SLGIMASSRG + GRL++Q
Sbjct: 121 TLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVVQ 177

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BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 3.2e-06
Identity = 44/154 (28.57%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 24  ILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSH 83
           + +  EV+  I+  + + ++ L  +  PA +    ID RSS     + I+ DDS      
Sbjct: 41  VASSSEVLTAIENIIQDIIKSLARNEVPAFT----IDNRSS----WENIMFDDSVGLRMI 100

Query: 84  SFCTRSLMR---PNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQL 143
             CT   +R   P + K F  + K++ M  +++  D   T+R+++Y    DS + F  Q 
Sbjct: 101 PQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYY---TDSQL-FGNQA 160

Query: 144 HVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRL 174
            V+  I D+  +L+  R SL ++++S+GL+AG L
Sbjct: 161 AVDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNL 182

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BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.2e-06
Identity = 44/154 (28.57%), Postives = 82/154 (53.25%), Query Frame = 0


Query: 24  ILTPQEVVARIQVAVLNFLRIL-NSSSPAISNLPLIDRRSSNSRVSQGILTDDSWIFLSH 83
           + +  EV+A I+  + + +  L  + +PA +    ID RSS     + I  +DS      
Sbjct: 41  LASSSEVLASIENIIQDIITSLARNEAPAFT----IDNRSS----WENIKFEDSVGLQMV 100

Query: 84  SFCTRSLMR---PNASKAFVRVWKLMEMCSQILIQDKKVTQRELFYKLLCDSPVYFSTQL 143
           S CT   ++   P +++ F  + K++ M  +++  +   T+R+++Y    DS + F  Q 
Sbjct: 101 SHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYY---TDSQL-FGNQT 160

Query: 144 HVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRL 174
            V+  I D+  +L+ SR SL I+++S+GL+AG L
Sbjct: 161 VVDNIINDISCMLKVSRRSLHILSTSKGLIAGNL 182

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BLAST of Cucsat.G21342 vs. NCBI nr
Match: KAA0042721.1 (meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa])

HSP 1 Score: 355 bits (912), Expect = 5.09e-120
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHC 
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 VFLIGL 186
            FLIGL
Sbjct: 181 AFLIGL 186

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BLAST of Cucsat.G21342 vs. NCBI nr
Match: XP_004143970.1 (meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654718.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_031741008.1 meiotic recombination protein SPO11-2 [Cucumis sativus] >KGN50057.1 hypothetical protein Csa_000678 [Cucumis sativus])

HSP 1 Score: 342 bits (878), Expect = 5.31e-115
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. NCBI nr
Match: XP_008437337.1 (PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437341.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437342.1 PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo])

HSP 1 Score: 339 bits (870), Expect = 8.70e-114
Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. NCBI nr
Match: XP_038906477.1 (meiotic recombination protein SPO11-2 isoform X2 [Benincasa hispida])

HSP 1 Score: 320 bits (819), Expect = 1.12e-107
Identity = 163/176 (92.61%), Postives = 169/176 (96.02%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEP GLF+SSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPTGLFKSSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRV+QGILTDD WIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVNQGILTDDLWIFLSHSFCTRSLMRSNAAKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALLQCSR SLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLQCSRCSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. NCBI nr
Match: KAG7036015.1 (Meiotic recombination protein SPO11-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321 bits (822), Expect = 1.89e-106
Identity = 160/180 (88.89%), Postives = 174/180 (96.67%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           ME  GLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL+SS+PAISNLPLIDR
Sbjct: 1   METTGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILSSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
            SSNSRV+QGILTD+SWIFLSHSFCTRSLMR NA+KAFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  SSSNSRVNQGILTDESWIFLSHSFCTRSLMRANAAKAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSR+SLG+MASSRGLVAGRLLLQAHC+
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRFSLGVMASSRGLVAGRLLLQAHCS 180

[Close]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL
Match: A0A5A7TMD5 (Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002190 PE=3 SV=1)

HSP 1 Score: 355 bits (912), Expect = 2.47e-120
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCA 180
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHC 
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQAHCT 180

Query: 181 VFLIGL 186
            FLIGL
Sbjct: 181 AFLIGL 186

[Close]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL
Match: A0A0A0KNU2 (TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3 SV=1)

HSP 1 Score: 342 bits (878), Expect = 2.57e-115
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL
Match: A0A1S3AUC6 (meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE=3 SV=1)

HSP 1 Score: 339 bits (870), Expect = 4.21e-114
Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQ+AVLNFLRILNSSSPAISNLPLIDR
Sbjct: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQIAVLNFLRILNSSSPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDK+VTQ
Sbjct: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL
Match: A0A6J1DX60 (meiotic recombination protein SPO11-2 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 312 bits (799), Expect = 6.00e-105
Identity = 158/176 (89.77%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPAGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNS V++GILTD SWI+LSHSFCTRSLMR NA++AFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  RSSNSGVNRGILTDVSWIYLSHSFCTRSLMRANAAEAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLLQ 176

[Close]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL
Match: A0A6J1DSB8 (meiotic recombination protein SPO11-2 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111023944 PE=3 SV=1)

HSP 1 Score: 312 bits (799), Expect = 6.87e-105
Identity = 158/176 (89.77%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 1   MEPAGLFESSMRFFSDQELCYADILTPQEVVARIQVAVLNFLRILNSSSPAISNLPLIDR 60
           MEPAGLF+SSMRFFSDQELCYADILTPQEV+ARIQVAVLNFLRIL SS+PAISNLPLIDR
Sbjct: 1   MEPAGLFKSSMRFFSDQELCYADILTPQEVLARIQVAVLNFLRILTSSTPAISNLPLIDR 60

Query: 61  RSSNSRVSQGILTDDSWIFLSHSFCTRSLMRPNASKAFVRVWKLMEMCSQILIQDKKVTQ 120
           RSSNS V++GILTD SWI+LSHSFCTRSLMR NA++AFVRVWKLMEMCSQIL+Q+K+VTQ
Sbjct: 61  RSSNSGVNRGILTDVSWIYLSHSFCTRSLMRANAAEAFVRVWKLMEMCSQILLQEKRVTQ 120

Query: 121 RELFYKLLCDSPVYFSTQLHVNRTIQDVVALLQCSRYSLGIMASSRGLVAGRLLLQ 176
           RELFYKLLCDSP YF TQLHVNRTIQDVVALL+CSRYSLGIMASSRGLVAGRLLLQ
Sbjct: 121 RELFYKLLCDSPAYFPTQLHVNRTIQDVVALLRCSRYSLGIMASSRGLVAGRLLLQ 176

The following BLAST results are available for this feature:

BLAST of Cucsat.G21342 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A1	5.9e-61	70.86	Meiotic recombination protein SPO11-2 OS=Arabidopsis thaliana OX=3702 GN=SPO11-2...	[more]
Q5ZPI9	5.3e-46	58.43	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q6ZD95	1.2e-45	57.87	Meiotic recombination protein SPO11-2 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9WTK8	3.2e-06	28.57	Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2	[more]
Q9Y5K1	9.2e-06	28.57	Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1	[more]

BLAST of Cucsat.G21342 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0042721.1	5.09e-120	97.31	meiotic recombination protein SPO11-2 [Cucumis melo var. makuwa]	[more]
XP_004143970.1	5.31e-115	100.00	meiotic recombination protein SPO11-2 [Cucumis sativus] >XP_011654717.1 meiotic ...	[more]
XP_008437337.1	8.70e-114	98.30	PREDICTED: meiotic recombination protein SPO11-2 [Cucumis melo] >XP_008437338.1 ...	[more]
XP_038906477.1	1.12e-107	92.61	meiotic recombination protein SPO11-2 isoform X2 [Benincasa hispida]	[more]
KAG7036015.1	1.89e-106	88.89	Meiotic recombination protein SPO11-2 [Cucurbita argyrosperma subsp. argyrosperm...	[more]

BLAST of Cucsat.G21342 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TMD5	2.47e-120	97.31	Meiotic recombination protein SPO11-2 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0KNU2	2.57e-115	100.00	TP6A_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152220 PE=3...	[more]
A0A1S3AUC6	4.21e-114	98.30	meiotic recombination protein SPO11-2 OS=Cucumis melo OX=3656 GN=LOC103482788 PE...	[more]
A0A6J1DX60	6.00e-105	89.77	meiotic recombination protein SPO11-2 isoform X4 OS=Momordica charantia OX=3673 ...	[more]
A0A6J1DSB8	6.87e-105	89.77	meiotic recombination protein SPO11-2 isoform X3 OS=Momordica charantia OX=3673 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 18..39 score: 45.74 coord: 51..67 score: 48.53
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 8..163
None	No IPR available	GENE3D	3.40.1360.10		coord: 5..164 e-value: 1.0E-47 score: 164.5
None	No IPR available	PANTHER	PTHR10848:SF0	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 8..163
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 8..149 e-value: 2.44088E-50 score: 157.031
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 9..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21342.T2	Cucsat.G21342.T2	mRNA
Cucsat.G21342.T1	Cucsat.G21342.T1	mRNA
Cucsat.G21342.T15	Cucsat.G21342.T15	mRNA
Cucsat.G21342.T11	Cucsat.G21342.T11	mRNA
Cucsat.G21342.T12	Cucsat.G21342.T12	mRNA
Cucsat.G21342.T3	Cucsat.G21342.T3	mRNA
Cucsat.G21342.T6	Cucsat.G21342.T6	mRNA
Cucsat.G21342.T5	Cucsat.G21342.T5	mRNA
Cucsat.G21342.T22	Cucsat.G21342.T22	mRNA
Cucsat.G21342.T21	Cucsat.G21342.T21	mRNA
Cucsat.G21342.T7	Cucsat.G21342.T7	mRNA
Cucsat.G21342.T18	Cucsat.G21342.T18	mRNA
Cucsat.G21342.T14	Cucsat.G21342.T14	mRNA
Cucsat.G21342.T16	Cucsat.G21342.T16	mRNA
Cucsat.G21342.T17	Cucsat.G21342.T17	mRNA
Cucsat.G21342.T9	Cucsat.G21342.T9	mRNA
Cucsat.G21342.T8	Cucsat.G21342.T8	mRNA
Cucsat.G21342.T20	Cucsat.G21342.T20	mRNA
Cucsat.G21342.T26	Cucsat.G21342.T26	mRNA
Cucsat.G21342.T10	Cucsat.G21342.T10	mRNA
Cucsat.G21342.T27	Cucsat.G21342.T27	mRNA
Cucsat.G21342.T23	Cucsat.G21342.T23	mRNA
Cucsat.G21342.T13	Cucsat.G21342.T13	mRNA
Cucsat.G21342.T4	Cucsat.G21342.T4	mRNA
Cucsat.G21342.T19	Cucsat.G21342.T19	mRNA
Cucsat.G21342.T24	Cucsat.G21342.T24	mRNA
Cucsat.G21342.T25	Cucsat.G21342.T25	mRNA
Cucsat.G21342.T28	Cucsat.G21342.T28	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0009553	embryo sac development
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0000706	meiotic DNA double-strand break processing
biological_process	GO:0009555	pollen development
biological_process	GO:0048316	seed development
biological_process	GO:0006259	DNA metabolic process
cellular_component	GO:0000228	nuclear chromosome
cellular_component	GO:0005694	chromosome
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity