Cucsat.G21164.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G21164.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAcyl-coenzyme A oxidase
Locationctg910: 2931618 .. 2934776 (-)
RNA-Seq ExpressionCucsat.G21164.T4
SyntenyCucsat.G21164.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGGAAAAGAAAAAAAAAATCAAAACCGAAGACCTTTTCATTGGGAAACACAACGACCGACAAGTCGTGTGGATCGGAGTATTATCTCTTTTCTTTTCCTAATGGGATATTGAGAATCATTCTTCTTTAAATACCCACAAACCTCCATTCCATTTGGCCGAGATTTTGCAGCTCGTTTTCGATTTCCGACATCGAATATTCAATTTCTGTTATTTTTCGATCTGGGGTCGTCGATTTCACTGATTTAAGCTCTTTTTTGAAGTGGGGTTTTCGAATTCTTTTTGACGAACAAATGGAGGGTGTTGATCATCTTGCCCATGAGAGGAACAGGTCCCAATTCGACGTTAATGACATGAAGATCGTTTGGGCTGGTTCTCGTGAAGCGTTTGAGGTATCTGATCGAATGTCTCGACTGGTTGCTAATGATCCGGTGAGTAATTTCTGTTCCACAGTCTTTAAATTTCTGTACATTCTTTAAATTTTTGTAATTCATTTGATTGAAAGGATTTTTTGTGTGATGTTTTTGGTTTAAAGGCCTTTCGAAAGGACAATAGGGTCCATTTAACAAGGAAGGAATTGTTTAAGAACACTCTTAGGAAAGCAGCTTATGCATGGAAGAAGATTATTGAGCTTAAACTTTCTGGTATGACTACTTGATTTGAGCTCCTTATGTGGATTATGTTGTATTGTATTCTTATTTTCTTGAACATTTAATCACTTCGTGTTGTTATTTCTTTAGAGGAGGAAGCTGGTAGATTAAGGTTCTTTGTGGATGAGCCAGCATATACGGATCTTCACTGGGTCAGTTGACTCTATTCTCTTTGTTGGCATTACAACAACAATTACAGGCTAAATTGTTGGCTGTATGACTTTTGTTTTGTTTGACAACTCTGTGGATAAATATCGGTCTCCATCATCTTGCCTTTTAAACTTTTGGAATTTCGTTTAATGTTCCATTTCTCATTCTCTGTTTTTTTCTTTGTTTTAGGGAATGTTCGTTCCGTTCCTTAAAGGTCAAGGTACTGAGGAGCAGCTACAGAAGTGGTTGCCATTGGCATATAAGATGCAAATAATTGGTTGCTATGCTCAAACCGAACTTGGTCATGGTTCAAATGTTCAAGGGCTTGAGACAACTGCAACGTTTGATCCAAAGACAGACGAATTTGTCATACACAGTCCTACACTAACCTCAAGCAAAGTAAGTACAGTGGTAACTGTATATTCAGGCAGGGGAAGTTAGTTACCTCTTACCTGTATCGGATTGATCTGTATCAAACCCTGTTTCATCTTTCTGTGCCAATAATTAAAGAATTTGTTTCCTTTAGGTGTGTAAATTGAATGGATTAGATTGATAATGAAGAGAAATTTTAATTAAATTTGCTATTTTGGTGCCTTGATCAACTGTAGAGTTGTAATCCAATAAATTGACTGTAGTTTATAATATCTTTTTGGTTGATAGTTGGTATTCACACTCAACATTGATGTTTTAACTTTGGCACCTCTGTATTTAGGTATGAGAATATGCCGCACATTCCTTGTCTCTTACTTTCATTCCTTCTTTCTTGTGAACAGTGGTGGCCTGGTGGTTTGGGTAAGGTTTCAACACATGCAGTTGTATTTGCTCGTCTTATTACCAACGGTCGAGATTATGGAGTGCATGGTATGCACACTATGAATGCTTTCATTCCTCTAGTTCTTGATCCTTATTTATTATTTCTAATCAGAAAGTTTCCATTTTGTAGGTTTCATAGTCCAGATAAGGAGTTTGGATGATCACTCAGTTCTACCAGGCATAACCATTGGAGATATTGGTGTGAAATTCGGGAATGGAGCTTACAATACTATGGATAATGGGGTCATGCACTTTGATCACATCCGCATCCCAAGGAACCAACTGTTGATGAGGTTATTTCTATTTTCTGTTTTATCTTGTTTGTTTTATCTATTCATTTTCTGTCACCTATTCCTTACTATCATATTATATCTCTCTATGTTTTTCTTGTACATTGTTCGTGTCTATCTATTGGGAATAGGAATGGGATCATTTTCCTTGTTGGTTCTTATTGCTTGATTTTTCTTGTTTGAGGGATGAGCCATAGCGAATCTTATTTCTATTATTCAATTTTACAAAAATTTTAAAAACAAAGAAAATACAGAAAGAACGACACTCTAAGTGGTGTGCATATATATCATGCATTCAGTCGATTAAGCCTTCTAAAGTTGGCTCTAAACTTCACAGGTTTTCTCAAGTAACAAGGGAAGGGAAATATGTGCAATCAGATGTTCCTCGACAACTAGTTTATGGCACCATGGTATACGTTAGAAAGACAATTGTAATTGATGCTTCAAATGCCTTATCACGTGCAGTCTGTATTGCAACAAGGTACAGTGCTGTTCGTAGACAATTTGGATCACAGGATGGTGTGGAAACTCAGGTATCAGTTTAGAAGATTCTATGTTAAGGGTATATTTGTAATTACCATTAATTTAGGCTGTTGTAGTCACGTTAACTAGTCTGTGTCAATTTTGTATCTAGTTAGATATATTTGTTTTTCTTTTCAAGAGTTTTATTTATTTTACGGTTTCGACCTGCAATTATTAACATTCTGCTCTGATTAATTCCCCTGGTAATTATTGCTAGTTTCTTGTGTGTAACCAACTCATCACAATTATTTTTTCCATATGGGTTCATGAAATCAAGACCAACATAAATACAAATAGTTGAGTATACAATTACCAATCTGTCTTAATGTCCATGTTAGAAGTAAAACATAGAAAAAATTGCCTCGACGACGTTAGTTTGCTGTGCCAGATTGTTGATGAATAATGTTTTCTCATGTGTTTGATCTTTTAGGTGATCAACTATAAAACGCAACAAAGTAGGCTATTCCCTTTGCTGGCTTCTGCCTATGCTTTCAGATTTGTTGGTGAGTGGTTACAATGGCTATACACGGATGTGACTCAGAGACTCGCTGCCAACGATTTCTCAACCTTGCCTGAGGCTCATGCATGCACTGCAGGGTTGAAGTCAATCACAACGGCTGCAACTGCTGTGAGTGTCTTTCTCTTGCAAGTCCTCTCAAGGACCAACCATTGGGTTGTGAGCATAATGTAGAAATGTTCAACTCCATAGTTCTTTTCTAAGAAAATCCTATACT

Coding sequence (CDS)

ATGGAGGGTGTTGATCATCTTGCCCATGAGAGGAACAGGTCCCAATTCGACGTTAATGACATGAAGATCGTTTGGGCTGGTTCTCGTGAAGCGTTTGAGGTATCTGATCGAATGTCTCGACTGGTTGCTAATGATCCGGCCTTTCGAAAGGACAATAGGGTCCATTTAACAAGGAAGGAATTGTTTAAGAACACTCTTAGGAAAGCAGCTTATGCATGGAAGAAGATTATTGAGCTTAAACTTTCTGAGGAGGAAGCTGGTAGATTAAGGTTCTTTGTGGATGAGCCAGCATATACGGATCTTCACTGGGGAATGTTCGTTCCGTTCCTTAAAGGTCAAGGTACTGAGGAGCAGCTACAGAAGTGGTTGCCATTGGCATATAAGATGCAAATAATTGGTTGCTATGCTCAAACCGAACTTGGTCATGGTTCAAATGTTCAAGGGCTTGAGACAACTGCAACGTTTGATCCAAAGACAGACGAATTTGTCATACACAGTCCTACACTAACCTCAAGCAAATGGTGGCCTGGTGGTTTGGGTAAGGTTTCAACACATGCAGTTGTATTTGCTCGTCTTATTACCAACGGTCGAGATTATGGAGTGCATGGTTTCATAGTCCAGATAAGGAGTTTGGATGATCACTCAGTTCTACCAGGCATAACCATTGGAGATATTGGTGTGAAATTCGGGAATGGAGCTTACAATACTATGGATAATGGGGTCATGCACTTTGATCACATCCGCATCCCAAGGAACCAACTGTTGATGAGGTTTTCTCAAGTAACAAGGGAAGGGAAATATGTGCAATCAGATGTTCCTCGACAACTAGTTTATGGCACCATGGTATACGTTAGAAAGACAATTGTAATTGATGCTTCAAATGCCTTATCACGTGCAGTCTGTATTGCAACAAGGTACAGTGCTGTTCGTAGACAATTTGGATCACAGGATGGTGTGGAAACTCAGGTGATCAACTATAAAACGCAACAAAGTAGGCTATTCCCTTTGCTGGCTTCTGCCTATGCTTTCAGATTTGTTGGTGAGTGGTTACAATGGCTATACACGGATGTGACTCAGAGACTCGCTGCCAACGATTTCTCAACCTTGCCTGAGGCTCATGCATGCACTGCAGGGTTGAAGTCAATCACAACGGCTGCAACTGCTGTGAGTGTCTTTCTCTTGCAAGTCCTCTCAAGGACCAACCATTGGGTTGTGAGCATAATGTAG

Protein sequence

MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKELFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQKWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLGKVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNGVMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAVCIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQRLAANDFSTLPEAHACTAGLKSITTAATAVSVFLLQVLSRTNHWVVSIM
Homology
BLAST of Cucsat.G21164.T4 vs. ExPASy Swiss-Prot
Match: O65202 (Peroxisomal acyl-coenzyme A oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=ACX1 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 1.8e-189
Identity = 316/389 (81.23%), Postives = 361/389 (92.80%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEG+DHLA ERN+++FDV DMKIVWAGSR AFEVSDR++RLVA+DP F K NR  L+RKE
Sbjct: 1   MEGIDHLADERNKAEFDVEDMKIVWAGSRHAFEVSDRIARLVASDPVFEKSNRARLSRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFK+TLRK A+A+K+IIEL+L+EEEAGRLR F+D+PAY DLHWGMFVP +KGQGTEEQ +
Sbjct: 61  LFKSTLRKCAHAFKRIIELRLNEEEAGRLRHFIDQPAYVDLHWGMFVPAIKGQGTEEQQK 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWL LA KMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIH+PT T+SKWWPGGLG
Sbjct: 121 KWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTPTQTASKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVV+ARLITNG+DYG+HGFIVQ+RSL+DHS LP IT+GDIG K GNGAYN+MDNG
Sbjct: 181 KVSTHAVVYARLITNGKDYGIHGFIVQLRSLEDHSPLPNITVGDIGTKMGNGAYNSMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
            + FDH+RIPR+Q+LMR S+VTREG+YV SDVP+QLVYGTMVYVR+TIV DASNALSRAV
Sbjct: 241 FLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVPKQLVYGTMVYVRQTIVADASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQD-GVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQ 360
           CIATRYSAVRRQFG+ + G+ETQVI+YKTQQ+RLFPLLASAYAFRFVGEWL+WLYTDVT+
Sbjct: 301 CIATRYSAVRRQFGAHNGGIETQVIDYKTQQNRLFPLLASAYAFRFVGEWLKWLYTDVTE 360

Query: 361 RLAANDFSTLPEAHACTAGLKSITTAATA 389
           RLAA+DF+TLPEAHACTAGLKS+TT ATA
Sbjct: 361 RLAASDFATLPEAHACTAGLKSLTTTATA 389

BLAST of Cucsat.G21164.T4 vs. ExPASy Swiss-Prot
Match: Q9ZQP2 (Putative peroxisomal acyl-coenzyme A oxidase 1.2 OS=Arabidopsis thaliana OX=3702 GN=ACX1.2 PE=2 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 1.3e-184
Identity = 310/389 (79.69%), Postives = 356/389 (91.52%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           ME VDHLA ERN+++F+V+DMKIVWAGSR AF+VS+RMSRLVANDP F K  R  ++RKE
Sbjct: 1   MERVDHLADERNKAEFNVDDMKIVWAGSRHAFDVSNRMSRLVANDPVFEKSKRAVMSRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRK+ +AWK I EL+LS+EE  +LR F+D+P + DLHWGMFVP +KGQGTE+Q Q
Sbjct: 61  LFKNTLRKSVHAWKLINELRLSDEEGLKLRSFMDQPGFLDLHWGMFVPAIKGQGTEQQQQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWL LA KMQIIGCYAQTELGHGSNVQGLETTATFDPKTD+F+IHSPT TSSKWWPGGLG
Sbjct: 121 KWLSLATKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDQFIIHSPTQTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAV++ARLITNG+D+GVHGFIVQ+RSLDDHS LPGIT+GDIG+KFGNGAYN+MDNG
Sbjct: 181 KVSTHAVIYARLITNGKDHGVHGFIVQLRSLDDHSPLPGITVGDIGMKFGNGAYNSMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
            + FDH RIPR+Q+LMR S+VTREGKYV SDVPRQLVYGTMVYVR++IV +AS AL+RAV
Sbjct: 241 FLMFDHFRIPRDQMLMRLSKVTREGKYVASDVPRQLVYGTMVYVRQSIVSNASTALARAV 300

Query: 301 CIATRYSAVRRQFGSQD-GVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQ 360
           CIATRYSAVRRQFGS D G+ETQVINYKTQQ+RLFPLLASAYAFRFVGEWL+WLYTDVT+
Sbjct: 301 CIATRYSAVRRQFGSHDGGIETQVINYKTQQNRLFPLLASAYAFRFVGEWLKWLYTDVTK 360

Query: 361 RLAANDFSTLPEAHACTAGLKSITTAATA 389
           RL A+DF+TLPEAHACTAGLKS+TT+AT+
Sbjct: 361 RLEASDFATLPEAHACTAGLKSMTTSATS 389

BLAST of Cucsat.G21164.T4 vs. ExPASy Swiss-Prot
Match: P07872 (Peroxisomal acyl-coenzyme A oxidase 1 OS=Rattus norvegicus OX=10116 GN=Acox1 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.9e-90
Identity = 175/382 (45.81%), Postives = 250/382 (65.45%), Query Frame = 0

Query: 7   LAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKELFKNTL 66
           L  ER  + F+   +  +  GS E       +  L+ NDP F+ ++   LTR + ++  +
Sbjct: 5   LRKERASATFNPELITHILDGSPENTRRRREIENLILNDPDFQHEDYNFLTRSQRYEVAV 64

Query: 67  RKAAYAWKKIIELKLSEEEA---GRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQKWL 126
           +K+A   KK+ E  +S+ E     +  +  +      L++ MF+P L  QGT  Q +KW+
Sbjct: 65  KKSATMVKKMREYGISDPEEIMWFKKLYLANFVEPVGLNYSMFIPTLLNQGTTAQQEKWM 124

Query: 127 PLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLGKVS 186
             + ++QIIG YAQTE+GHG++++GLETTAT+DPKT EF+++SPT+TS KWWPGGLGK S
Sbjct: 125 RPSQELQIIGTYAQTEMGHGTHLRGLETTATYDPKTQEFILNSPTVTSIKWWPGGLGKTS 184

Query: 187 THAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNGVMH 246
            HA+V A+LIT G  YG+H F+V IR +  H  LPGIT+GDIG KFG   Y  MDNG + 
Sbjct: 185 NHAIVLAQLITQGECYGLHAFVVPIREIGTHKPLPGITVGDIGPKFG---YEEMDNGYLK 244

Query: 247 FDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAVCIA 306
            D+ RIPR  +LM+++QV  +G YV+  +  +L YGTMV+VR  +V +A+ +LS+A  IA
Sbjct: 245 MDNYRIPRENMLMKYAQVKPDGTYVK-PLSNKLTYGTMVFVRSFLVGNAAQSLSKACTIA 304

Query: 307 TRYSAVRRQFG-SQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQRLA 366
            RYSAVRRQ    Q   E Q+++++TQQ +LFPLLA+AYAF FVG +++  Y  + + + 
Sbjct: 305 IRYSAVRRQSEIKQSEPEPQILDFQTQQYKLFPLLATAYAFHFVGRYMKETYLRINESIG 364

Query: 367 ANDFSTLPEAHACTAGLKSITT 385
             D S LPE HA TAGLK+ TT
Sbjct: 365 QGDLSELPELHALTAGLKAFTT 382

BLAST of Cucsat.G21164.T4 vs. ExPASy Swiss-Prot
Match: Q5RC19 (Peroxisomal acyl-coenzyme A oxidase 1 OS=Pongo abelii OX=9601 GN=ACOX1 PE=2 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 1.2e-89
Identity = 176/389 (45.24%), Postives = 256/389 (65.81%), Query Frame = 0

Query: 7   LAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKELFKNTL 66
           L  ER+ + F+   +  +  GS E       +  ++ NDP F+ ++   LTR + ++  +
Sbjct: 5   LRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAV 64

Query: 67  RKAAYAWKKIIELKLSEEEA---GRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQKWL 126
           RK+A   KK+ E  +++ +     +    V+      L++ MF+P L  QGT  Q +KWL
Sbjct: 65  RKSAIMVKKMREFGIADPDEIMWFKKLHLVNFVEPVGLNYSMFIPTLLNQGTTAQKEKWL 124

Query: 127 PLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLGKVS 186
             +  +QIIG YAQTE+GHG++++GLETTAT+DP+T EF+++SPT+TS KWWPGGLGK S
Sbjct: 125 LSSKGLQIIGTYAQTEMGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTS 184

Query: 187 THAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNGVMH 246
            HA+V A+LIT G+ YG+H FIV IR +  H  LPGIT+GDIG KFG   Y+ +DNG + 
Sbjct: 185 NHAIVLAQLITKGKGYGLHAFIVPIREIGTHKPLPGITVGDIGPKFG---YDEIDNGYLK 244

Query: 247 FDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAVCIA 306
            D+ RIPR  +LM+++QV  +G YV+  +  +L YGTMV+VR  +V +A+ ALS+A  IA
Sbjct: 245 MDNYRIPRENMLMKYAQVKPDGTYVK-PLSNKLTYGTMVFVRSFLVGEAARALSKACTIA 304

Query: 307 TRYSAVRRQFGSQDG-VETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQRLA 366
            RYSAVR Q   + G  E Q+++++TQQ +LFPLLA+AYAF+FVG +++  Y  + + + 
Sbjct: 305 IRYSAVRHQSEIKPGEPEPQILDFQTQQYKLFPLLATAYAFQFVGAYMKETYHRINEGIG 364

Query: 367 ANDFSTLPEAHACTAGLKSIT--TAATAV 390
             D S LPE HA TAGLK+ T  TA T +
Sbjct: 365 QGDLSELPELHALTAGLKAFTSWTANTGI 389

BLAST of Cucsat.G21164.T4 vs. ExPASy Swiss-Prot
Match: Q9Z1N0 (Peroxisomal acyl-coenzyme A oxidase 1 OS=Cavia porcellus OX=10141 GN=ACOX1 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.6e-89
Identity = 181/385 (47.01%), Postives = 251/385 (65.19%), Query Frame = 0

Query: 7   LAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKELFKNTL 66
           L  ER  + F+   +  +  GS E       +   + +DP F+ +N   LTR E ++  +
Sbjct: 5   LRRERAAATFNPELITHLLDGSPEKTRRRREIENKILSDPDFQHENHNFLTRSERYEAAI 64

Query: 67  RKAAYAWKKIIELKLSEEEAGR------LRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 126
           +K+A   KK+ E  +++ +         L  FV EP    L++ MF+P L  QGT  Q +
Sbjct: 65  KKSAVMVKKMREFGIADPDEIMWFKRLLLGNFV-EP--VGLNYSMFIPTLLNQGTTAQQE 124

Query: 127 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 186
           KWL  +  +QIIG YAQTE+GHG++++GLETTAT+DPKT EF+I+SPT+TS KWWPGGLG
Sbjct: 125 KWLHPSTGLQIIGTYAQTEMGHGTHLRGLETTATYDPKTQEFIINSPTVTSIKWWPGGLG 184

Query: 187 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 246
           K S HA+V A+LIT G+ YG+H FIV IR +  H  LPGIT+GDIG KFG   Y  MDNG
Sbjct: 185 KTSNHAIVLAQLITQGKCYGLHAFIVPIREIGTHKPLPGITVGDIGPKFG---YEEMDNG 244

Query: 247 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 306
            +  D+ RIPR  +LM+++QV  +G YV+  V  +L YGTMV+VR  +V  A+ +LS+A 
Sbjct: 245 YLKMDNYRIPRENMLMKYAQVKPDGTYVK-PVSNKLTYGTMVFVRSFLVGAAAQSLSKAC 304

Query: 307 CIATRYSAVRRQFGSQDG-VETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQ 366
            IA RYSAVR Q   + G  E QV++++TQQ +LFP+LA+AYAF+FVG +++  Y  + +
Sbjct: 305 TIAIRYSAVRHQSEIKPGEPEPQVLDFQTQQYKLFPILATAYAFQFVGSYMKDTYHRINE 364

Query: 367 RLAANDFSTLPEAHACTAGLKSITT 385
            +   D S LPE HA TAGLK+ TT
Sbjct: 365 SIGQGDLSELPELHALTAGLKAFTT 382

BLAST of Cucsat.G21164.T4 vs. NCBI nr
Match: XP_004152392.3 (peroxisomal acyl-coenzyme A oxidase 1 [Cucumis sativus] >KGN49757.2 hypothetical protein Csa_000028 [Cucumis sativus])

HSP 1 Score: 783 bits (2023), Expect = 3.30e-281
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. NCBI nr
Match: XP_008437050.1 (PREDICTED: peroxisomal acyl-coenzyme A oxidase 1 [Cucumis melo])

HSP 1 Score: 775 bits (2000), Expect = 1.04e-277
Identity = 382/388 (98.45%), Postives = 386/388 (99.48%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERN++QFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNKAQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQL+
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLK 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDG E QVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGAENQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. NCBI nr
Match: KAA0039733.1 (peroxisomal acyl-coenzyme A oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 771 bits (1991), Expect = 2.43e-276
Identity = 381/388 (98.20%), Postives = 385/388 (99.23%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERN++QFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNKAQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQL+
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLK 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLA KMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLASKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDG E QVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGAENQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. NCBI nr
Match: XP_038907136.1 (peroxisomal acyl-coenzyme A oxidase 1-like [Benincasa hispida])

HSP 1 Score: 766 bits (1977), Expect = 3.27e-274
Identity = 376/388 (96.91%), Postives = 384/388 (98.97%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERN +QFDVNDMKIVWAGSR+AFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNMAQFDVNDMKIVWAGSRQAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWK+IIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ
Sbjct: 61  LFKNTLRKAAYAWKRIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFD KTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDAKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNGRDYGVHGF+VQIRSLDDHS LPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGRDYGVHGFMVQIRSLDDHSALPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VM FDH+RIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIV+DASNALSRAV
Sbjct: 241 VMRFDHVRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVVDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFG+QDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVT+R
Sbjct: 301 CIATRYSAVRRQFGAQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTER 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. NCBI nr
Match: XP_022995810.1 (peroxisomal acyl-coenzyme A oxidase 1-like [Cucurbita maxima])

HSP 1 Score: 748 bits (1931), Expect = 3.22e-267
Identity = 367/388 (94.59%), Postives = 379/388 (97.68%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERNRSQFDV+ MKIVWAGSR AFEVSDRMSRLVANDPAFRKDNR  L RKE
Sbjct: 1   MEGVDHLAHERNRSQFDVDAMKIVWAGSRHAFEVSDRMSRLVANDPAFRKDNRAQLPRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRK AYAWKKI+ELKLSEEEA RLRFFVDEPAYTDLHWGMFVPF+KGQGTEEQLQ
Sbjct: 61  LFKNTLRKTAYAWKKIVELKLSEEEAARLRFFVDEPAYTDLHWGMFVPFVKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKM+IIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMKIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSL+DHSVLPGITIGDIG+KFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLEDHSVLPGITIGDIGMKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDH RIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHFRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQ+GVETQVI+YKTQQSRLFPLLASAYAFRFVGEWL+WLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQNGVETQVIDYKTQQSRLFPLLASAYAFRFVGEWLEWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           L A+DFSTLPEAHACTAGLKS+TTAATA
Sbjct: 361 LGASDFSTLPEAHACTAGLKSVTTAATA 388

BLAST of Cucsat.G21164.T4 vs. ExPASy TrEMBL
Match: A0A1S3ATN1 (Acyl-coenzyme A oxidase OS=Cucumis melo OX=3656 GN=LOC103482592 PE=3 SV=1)

HSP 1 Score: 775 bits (2000), Expect = 5.03e-278
Identity = 382/388 (98.45%), Postives = 386/388 (99.48%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERN++QFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNKAQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQL+
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLK 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDG E QVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGAENQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. ExPASy TrEMBL
Match: A0A5A7T9P3 (Acyl-coenzyme A oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00650 PE=3 SV=1)

HSP 1 Score: 771 bits (1991), Expect = 1.18e-276
Identity = 381/388 (98.20%), Postives = 385/388 (99.23%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERN++QFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNKAQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQL+
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLK 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLA KMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLASKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDG E QVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGAENQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388

BLAST of Cucsat.G21164.T4 vs. ExPASy TrEMBL
Match: A0A0A0KRC5 (Acyl-coenzyme A oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G165210 PE=3 SV=1)

HSP 1 Score: 751 bits (1940), Expect = 4.52e-269
Identity = 376/388 (96.91%), Postives = 376/388 (96.91%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE
Sbjct: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ
Sbjct: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKMQIIGCYAQTELGHGSNVQ LETTATFDPKTDEF           WWPGGLG
Sbjct: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQWLETTATFDPKTDEF-----------WWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           LAANDFSTLPEAHACTAGLKSITTAATA
Sbjct: 361 LAANDFSTLPEAHACTAGLKSITTAATA 377

BLAST of Cucsat.G21164.T4 vs. ExPASy TrEMBL
Match: A0A6J1K4Y9 (Acyl-coenzyme A oxidase OS=Cucurbita maxima OX=3661 GN=LOC111491234 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 1.56e-267
Identity = 367/388 (94.59%), Postives = 379/388 (97.68%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERNRSQFDV+ MKIVWAGSR AFEVSDRMSRLVANDPAFRKDNR  L RKE
Sbjct: 1   MEGVDHLAHERNRSQFDVDAMKIVWAGSRHAFEVSDRMSRLVANDPAFRKDNRAQLPRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRK AYAWKKI+ELKLSEEEA RLRFFVDEPAYTDLHWGMFVPF+KGQGTEEQLQ
Sbjct: 61  LFKNTLRKTAYAWKKIVELKLSEEEAARLRFFVDEPAYTDLHWGMFVPFVKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKM+IIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMKIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSL+DHSVLPGITIGDIG+KFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLEDHSVLPGITIGDIGMKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDH RIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHFRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQ+GVETQVI+YKTQQSRLFPLLASAYAFRFVGEWL+WLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQNGVETQVIDYKTQQSRLFPLLASAYAFRFVGEWLEWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           L A+DFSTLPEAHACTAGLKS+TTAATA
Sbjct: 361 LGASDFSTLPEAHACTAGLKSVTTAATA 388

BLAST of Cucsat.G21164.T4 vs. ExPASy TrEMBL
Match: A0A6J1H1J6 (Acyl-coenzyme A oxidase OS=Cucurbita moschata OX=3662 GN=LOC111459236 PE=3 SV=1)

HSP 1 Score: 746 bits (1925), Expect = 1.27e-266
Identity = 365/388 (94.07%), Postives = 379/388 (97.68%), Query Frame = 0

Query: 1   MEGVDHLAHERNRSQFDVNDMKIVWAGSREAFEVSDRMSRLVANDPAFRKDNRVHLTRKE 60
           MEGVDHLAHERNRSQFDV+ MKIVWAGSR AFEVSDRMSRLVANDPAFRKDNR  L RKE
Sbjct: 1   MEGVDHLAHERNRSQFDVDAMKIVWAGSRHAFEVSDRMSRLVANDPAFRKDNRAQLPRKE 60

Query: 61  LFKNTLRKAAYAWKKIIELKLSEEEAGRLRFFVDEPAYTDLHWGMFVPFLKGQGTEEQLQ 120
           LFKNTLRK AYAWKKI+ELKL+EEEA RLRFFVDEPAYTDLHWGMFVPF+KGQGTEEQLQ
Sbjct: 61  LFKNTLRKTAYAWKKIVELKLTEEEAARLRFFVDEPAYTDLHWGMFVPFVKGQGTEEQLQ 120

Query: 121 KWLPLAYKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180
           KWLPLAYKM+IIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG
Sbjct: 121 KWLPLAYKMKIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHSPTLTSSKWWPGGLG 180

Query: 181 KVSTHAVVFARLITNGRDYGVHGFIVQIRSLDDHSVLPGITIGDIGVKFGNGAYNTMDNG 240
           KVSTHAVVFARLITNG+DYGVHGFIVQIRSL+DH+VLPGITIGDIG+KFGNGAYNTMDNG
Sbjct: 181 KVSTHAVVFARLITNGQDYGVHGFIVQIRSLEDHTVLPGITIGDIGMKFGNGAYNTMDNG 240

Query: 241 VMHFDHIRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300
           VMHFDH RIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV
Sbjct: 241 VMHFDHFRIPRNQLLMRFSQVTREGKYVQSDVPRQLVYGTMVYVRKTIVIDASNALSRAV 300

Query: 301 CIATRYSAVRRQFGSQDGVETQVINYKTQQSRLFPLLASAYAFRFVGEWLQWLYTDVTQR 360
           CIATRYSAVRRQFGSQ+GVETQVI+YKTQQSRLFPLLASAYAFRFVGEWL+WLYTDVTQR
Sbjct: 301 CIATRYSAVRRQFGSQNGVETQVIDYKTQQSRLFPLLASAYAFRFVGEWLEWLYTDVTQR 360

Query: 361 LAANDFSTLPEAHACTAGLKSITTAATA 388
           L A+DFSTLPEAHACTAGLKS+TTAATA
Sbjct: 361 LGASDFSTLPEAHACTAGLKSVTTAATA 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O652021.8e-18981.23Peroxisomal acyl-coenzyme A oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=ACX1 PE... [more]
Q9ZQP21.3e-18479.69Putative peroxisomal acyl-coenzyme A oxidase 1.2 OS=Arabidopsis thaliana OX=3702... [more]
P078721.9e-9045.81Peroxisomal acyl-coenzyme A oxidase 1 OS=Rattus norvegicus OX=10116 GN=Acox1 PE=... [more]
Q5RC191.2e-8945.24Peroxisomal acyl-coenzyme A oxidase 1 OS=Pongo abelii OX=9601 GN=ACOX1 PE=2 SV=2[more]
Q9Z1N01.6e-8947.01Peroxisomal acyl-coenzyme A oxidase 1 OS=Cavia porcellus OX=10141 GN=ACOX1 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_004152392.33.30e-281100.00peroxisomal acyl-coenzyme A oxidase 1 [Cucumis sativus] >KGN49757.2 hypothetical... [more]
XP_008437050.11.04e-27798.45PREDICTED: peroxisomal acyl-coenzyme A oxidase 1 [Cucumis melo][more]
KAA0039733.12.43e-27698.20peroxisomal acyl-coenzyme A oxidase 1 [Cucumis melo var. makuwa][more]
XP_038907136.13.27e-27496.91peroxisomal acyl-coenzyme A oxidase 1-like [Benincasa hispida][more]
XP_022995810.13.22e-26794.59peroxisomal acyl-coenzyme A oxidase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1S3ATN15.03e-27898.45Acyl-coenzyme A oxidase OS=Cucumis melo OX=3656 GN=LOC103482592 PE=3 SV=1[more]
A0A5A7T9P31.18e-27698.20Acyl-coenzyme A oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A0A0KRC54.52e-26996.91Acyl-coenzyme A oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G165210 PE=3 SV=1[more]
A0A6J1K4Y91.56e-26794.59Acyl-coenzyme A oxidase OS=Cucurbita maxima OX=3661 GN=LOC111491234 PE=3 SV=1[more]
A0A6J1H1J61.27e-26694.07Acyl-coenzyme A oxidase OS=Cucurbita moschata OX=3662 GN=LOC111459236 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006091Acyl-CoA oxidase/dehydrogenase, central domainPFAMPF02770Acyl-CoA_dh_Mcoord: 134..237
e-value: 2.5E-12
score: 46.8
NoneNo IPR availableGENE3D1.20.140.10coord: 279..392
e-value: 5.2E-35
score: 122.6
NoneNo IPR availableGENE3D2.40.110.10coord: 133..269
e-value: 3.0E-63
score: 213.9
NoneNo IPR availablePIRSRPIRSR000168-2PIRSR000168-2coord: 11..388
e-value: 1.3E-135
score: 450.9
NoneNo IPR availablePANTHERPTHR10909:SF385PEROXISOMAL ACYL-COENZYME A OXIDASE 1.2-RELATEDcoord: 1..391
IPR037069Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamilyGENE3D1.10.540.10coord: 17..132
e-value: 2.1E-35
score: 123.6
IPR029320Acyl-coenzyme A oxidase, N-terminalPFAMPF14749Acyl-CoA_ox_Ncoord: 17..132
e-value: 3.3E-29
score: 101.9
IPR012258Acyl-CoA oxidasePANTHERPTHR10909ELECTRON TRANSPORT OXIDOREDUCTASEcoord: 1..391
IPR036250Acyl-CoA dehydrogenase-like, C-terminalSUPERFAMILY47203Acyl-CoA dehydrogenase C-terminal domain-likecoord: 276..388
IPR009100Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamilySUPERFAMILY56645Acyl-CoA dehydrogenase NM domain-likecoord: 7..263

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G21164Cucsat.G21164gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21164.T4.E1Cucsat.G21164.T4.E1exon
Cucsat.G21164.T4.E2Cucsat.G21164.T4.E2exon
Cucsat.G21164.T4.E3Cucsat.G21164.T4.E3exon
Cucsat.G21164.T4.E4Cucsat.G21164.T4.E4exon
Cucsat.G21164.T4.E5Cucsat.G21164.T4.E5exon
Cucsat.G21164.T4.E6Cucsat.G21164.T4.E6exon
Cucsat.G21164.T4.E7Cucsat.G21164.T4.E7exon
Cucsat.G21164.T4.E8Cucsat.G21164.T4.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G21164.T4.C8Cucsat.G21164.T4.C8CDS
Cucsat.G21164.T4.C7Cucsat.G21164.T4.C7CDS
Cucsat.G21164.T4.C6Cucsat.G21164.T4.C6CDS
Cucsat.G21164.T4.C5Cucsat.G21164.T4.C5CDS
Cucsat.G21164.T4.C4Cucsat.G21164.T4.C4CDS
Cucsat.G21164.T4.C3Cucsat.G21164.T4.C3CDS
Cucsat.G21164.T4.C2Cucsat.G21164.T4.C2CDS
Cucsat.G21164.T4.C1Cucsat.G21164.T4.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G21164.T4Cucsat.G21164.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033540 fatty acid beta-oxidation using acyl-CoA oxidase
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0055088 lipid homeostasis
biological_process GO:0001676 long-chain fatty acid metabolic process
biological_process GO:0000038 very long-chain fatty acid metabolic process
cellular_component GO:0005777 peroxisome
molecular_function GO:0003997 acyl-CoA oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
molecular_function GO:0005504 fatty acid binding
molecular_function GO:0016401 palmitoyl-CoA oxidase activity