Cucsat.G20248.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G20248.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDiacylglycerol kinase
Locationctg47: 281181 .. 284625 (-)
RNA-Seq ExpressionCucsat.G20248.T1
SyntenyCucsat.G20248.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATCTAAAAAAAGGAGGATTGAAGGAAGCTCAATGTTTTTCGAATTTTTGTGTAAAGTCAGAATATTTAGTTGACATCAGACGTAAAGATGAGGAATTGTCTGACATCGGCCAGCGCAGTCCCTGTGATGCCAAACCAAAGTCCCCATCACCGACGCCACTCTGTTTACTCCTCACTCACAACCTTCCAACCTCCCGCTTTTATTGTTCCTACTCACCTAAGCTTTACTTCACCCCACAACAACAAAACACCAATCTTCCCCGCTTCTCAAACTCCCCACAAATGGACCATTCAAGCGCCGAACCCGGTGCCGGCGACGAAGTTACTGTAGCAGCTCGCTCCTCCTTCATCGACTCTCTCAAAGGCTGCGGCCTCTCCGGTATCCGAATCGATAAACATGAGCTCAATACAAAGGTTATAATGCCCCAATATCTCCGCTTTGCCTTGCGTGATTCTATCCGCTTGAAAGACCCCATTGCCGGAGCCTCTGCCACTACTCCGCCGCAGAATATGGTATCGCCATTGCCGGAGACCCCTTTGGTGGTCTTCATTAACCCACGTAGCGGTGGCCGCCATGGCCAGAAGCTTAAGCAGCGACTCCAGGAATTAATCAGCGAAGAACAGGTCCACTATTTATTCTGTGAAGAAGTCTCGGGATTCCCATTGTTTTTTCTTCTTTTTACGTGTGTCATCTTTGGAATTGCTGTACTTGTTTTTTGTTTGATTCTTATTTTTGGTGGGTGTAATTGTTGTATTTGAATTTCGTATCTGATTTTTTGTATGTATAATTGAGTGAGGATTTACTTCAGAGTTTGTATTGTACTGTTGTAGGTGTTTGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTATGGACTGCGTTGCCTGGAAATCTTGGCTGATCATGGCGATGTTTGTGCTAAACAAACTCGTCAAAAAATAAGGATCATGGTATTTTGATATTCACACTAACCCCACATAAAAAGATTGTTTCTATTGCTTTACATATTTGTTATCCAATGGAACTGTTTCGTATTGGAAAATTGAGGAAGAGAACTTGAACTGGTGGAGCAGGTTGCAGGAGGTGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGATCGCAACCCTGTTCTTCCTGTTGGGATTATTCCTCTTGGTACTGGGAATGATCTTGCCAGAAGTTTTGGTTGGGTGAGTTTTTGTAGTTTCAACTCGAAATTTTATAGGAAGGCATTATTTTTTCCTTTTCTACTCCAATTCAGAATCATTGCTTTGTTTTTGTTTTTTTTTTTAATCAGGGAGGTTCATTTCCTTTTGCTTGGAAATCTGCTGTTAAAAGATCTCTCCTCAGGGCTACCACTGGTCAAGTTGGTTCTTTGGATAGGTGAGAATTTGATTTTTTTTCTGAACTTCGAAGCTGTTTCTGGAGTTGGTTTTAAATTTGAATTTCTCTTGATGCAGTTGGCATGTTTCATTGTCGATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTTTGAAGCTAACAGAAGAAAGTGATCTGAATGAAGCTGTCCATGTAATTATATACTGTTTCAATTATACTATACCAGTTCCAATCACTTATCCACACAAAATAGAAGAAAATGTTGGAATTTATTTCTCAGTTTGTGATTGGAAATGTTGTCCCATGGCTGCAGAAACTGGATGACATAGAAGATTCACCTACCAAACTGACTTGTTTTGAAGGAGTATTTTATAATTACTTCAGCATAGGTAAGTTGAACTCACTGGTGAAGTTAAACTCTGAAATGTATATGTTTAGAGAAGTGATTAGTTGTAATTTCACTAGCAAAGTTGATTGTGTCTTGAACTTAATAGTCCCATGGAGGATTAGATTAATTAGTCATCATTCTGCTTCTCTTCTTTAAGACGATTGTCTATTTGCTTATCTGCAAAATCATTAGCAGTTGTTAATTAACCAATTCTATTAATGTACAATGCAGGAATGGATGCTCAAGTTGCCTACGGCTTCCACCATTTGCGCAACAAAAGACCGTACTTGGCTCAAGGTCCAGTTACAAATAAGGTTGAACTGACTATTATATTTATCCGTTAAACAAAACATGATTTCTTTTTTGATTAGTGGTTGTTTATAAATCTTTTATTTCTGACAGTTAATTTACTCAAGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAACAATCCAAATTTGAGGTGATAGCTTATACTTATTAAAAACCCAGTAGAATGCATATATATTCTCAGTTTCTAACCACATAAAGCTTTTCTTGTTGTAGTGGGCTTAAGAACATCATTAGAATACATATAAAAAAGATCAACTGTGCAGAATGGGAAAAGGTTCCAGTTCCTTCCAGGTAGAACTGCTCTCTCTACTCTCTTATGTGCGAATCGTGAAACTTGTTTACTTCTTTTTTGGGGGACATCAATTGATGTTGTTTTTTTGGTTGGGCTATAGTGTTAGGTCTATTGTTGCTTTAAACTTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCTAAAGCCAGAGTACTTGGAGAAGGTACTGACGACGAAGTTCTGTTATGCTATTGTGTTTTTTTAGTTAGCATTGTTGTTTTCTCTTCATGCTATACATTGACTGAAATATTTATTTTGTACTAGAGAGGTTTTGTGGAAGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTAAAACAAGGATGGCATACATCATTTGTAATGGTAGATATAATCTCTGCCAAACATATCGCCCAGGTGCTTCATCTTTTTCAGGAAAATTACTACTTGGTTTTCTACTCCAAACACGATTTGTGTTTTTGAACTGTTGAATGTTTATTTACAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGAAAGAAGGCCTATCTGCAAATGGATGGGGAGCCATGGAAACAACCAATAAGCAAGGAAAACTCGACGTTTGTTGAAATCAAGAGGGTCCCTTTTCAATCACTAATGATCAATGGGGACTAAAAGCTTCTTCATCTCTCATCATCTCCTCTTCAGTTTGCTGGCCGAGTTGATATATTCTTTTTTGTATGATATTATCATATATATGTATGTCTAAATTATTCATGTTCAACACAACCCTTAGAACATCCCTGAGGGCCTGAGGCTTAAAAGTATGTCTAGTAGGCAATCTGAAAATGGGAAGGTGAAAGTAAGAGATTTACTTATAACAGGGTTATATTTCATGTTTTTATACCTGTGTAAAGAAACTTAGGTCTCAATCAAAGTTATAATGCTTTTAAATTTGTACCAATTACTTTAGTAAGCAATAGTTCAGTCCTTAAATCTTCAATTTGTGACAATTTCGGTCCTTGTTTTAGAGTATGACAATATTGTTTAGA

Coding sequence (CDS)

TATCTAAAAAAAGGAGGATTGAAGGAAGCTCAATGTTTTTCGAATTTTTGTGTAAAGTCAGAATATTTAGTTGACATCAGACGTAAAGATGAGGAATTGTCTGACATCGGCCAGCGCAGTCCCTGTGATGCCAAACCAAAGTCCCCATCACCGACGCCACTCTGTTTACTCCTCACTCACAACCTTCCAACCTCCCGCTTTTATTGTTCCTACTCACCTAAGCTTTACTTCACCCCACAACAACAAAACACCAATCTTCCCCGCTTCTCAAACTCCCCACAAATGGACCATTCAAGCGCCGAACCCGGTGCCGGCGACGAAGTTACTGTAGCAGCTCGCTCCTCCTTCATCGACTCTCTCAAAGGCTGCGGCCTCTCCGGTATCCGAATCGATAAACATGAGCTCAATACAAAGGTTATAATGCCCCAATATCTCCGCTTTGCCTTGCGTGATTCTATCCGCTTGAAAGACCCCATTGCCGGAGCCTCTGCCACTACTCCGCCGCAGAATATGGTATCGCCATTGCCGGAGACCCCTTTGGTGGTCTTCATTAACCCACGTAGCGGTGGCCGCCATGGCCAGAAGCTTAAGCAGCGACTCCAGGAATTAATCAGCGAAGAACAGGTGTTTGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTATGGACTGCGTTGCCTGGAAATCTTGGCTGATCATGGCGATGTTTGTGCTAAACAAACTCGTCAAAAAATAAGGATCATGGTTGCAGGAGGTGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGATCGCAACCCTGTTCTTCCTGTTGGGATTATTCCTCTTGGTACTGGGAATGATCTTGCCAGAAGTTTTGGTTGGGGAGGTTCATTTCCTTTTGCTTGGAAATCTGCTGTTAAAAGATCTCTCCTCAGGGCTACCACTGGTCAAGTTGGTTCTTTGGATAGTTGGCATGTTTCATTGTCGATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTTTGAAGCTAACAGAAGAAAGTGATCTGAATGAAGCTGTCCATAAACTGGATGACATAGAAGATTCACCTACCAAACTGACTTGTTTTGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCCTACGGCTTCCACCATTTGCGCAACAAAAGACCGTACTTGGCTCAAGGTCCAGTTACAAATAAGTTAATTTACTCAAGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAACAATCCAAATTTGAGTGGGCTTAAGAACATCATTAGAATACATATAAAAAAGATCAACTGTGCAGAATGGGAAAAGGTTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACTTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCTAAAGCCAGAGTACTTGGAGAAGAGAGGTTTTGTGGAAGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTAAAACAAGGATGGCATACATCATTTGTAATGGTAGATATAATCTCTGCCAAACATATCGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGAAAGAAGGCCTATCTGCAAATGGATGGGGAGCCATGGAAACAACCAATAAGCAAGGAAAACTCGACGTTTGTTGAAATCAAGAGGGTCCCTTTTCAATCACTAATGATCAATGGGGACTAA

Protein sequence

YLKKGGLKEAQCFSNFCVKSEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTPQQQNTNLPRFSNSPQMDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSLMINGD
Homology
BLAST of Cucsat.G20248.T1 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.3e-187
Identity = 314/474 (66.24%), Postives = 382/474 (80.59%), Query Frame = 0

Query: 111 AARSSFIDSLKGCG---LSGIRIDKHELNTKVIMPQYLRFALRDSIRLKDPIAGASATTP 170
           +A++    +++GCG   L+ + IDK EL  ++ MP+YLR A+RD I+ KD    +     
Sbjct: 22  SAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLL 81

Query: 171 PQNMVSPL-PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLR 230
           P    + + P  P+VVFINP+SGGRHG  LK+RLQ+L++EEQVFDL +VKPHEFV+YGL 
Sbjct: 82  PGGAAADMAPHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLG 141

Query: 231 CLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTG 290
           CL+ LA  GD CA++ R+KIRIMVAGGDGTVGWVLG L EL+K  ++ + PVG+IPLGTG
Sbjct: 142 CLDTLAAKGDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTG 201

Query: 291 NDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAGEKVEPPHSLKLT 350
           NDL+RSF WGGSFPFAW+SA+KR+L RAT G +  LDSW + +SMP+GE V+PP+SLK T
Sbjct: 202 NDLSRSFSWGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPT 261

Query: 351 -EESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 410
            EE+ L++A   LD   D P K   +EGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGP
Sbjct: 262 IEETALDQA---LDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGP 321

Query: 411 VTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKVPVPSSVRSIVA 470
           VTNK+IYSSYSCTQGWF T C NNP L GL+NI++IHIKK NC+EWE++ VP SVRSIV 
Sbjct: 322 VTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVV 381

Query: 471 LNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 530
           LNL+NY SGR+PWG L+P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHI
Sbjct: 382 LNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHI 441

Query: 531 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSLMINGD 580
           AQAAAIR E+RGG  K A+LQMDGEPWKQP+  + STFVEIK+VPFQSLMING+
Sbjct: 442 AQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Cucsat.G20248.T1 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 9.9e-186
Identity = 311/491 (63.34%), Postives = 386/491 (78.62%), Query Frame = 0

Query: 95  MDHSSAEPGAGDEVTVAARSSFIDS--LKGCGLSG---IRIDKHELNTKVIMPQYLRFAL 154
           MD   ++  A  E  VA+R S  DS  ++GCGL+    + +DK EL  +++MP+YLR A+
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 155 RDSIRLKDPIAGASATTPPQNMVSPL-PETPLVVFINPRSGGRHGQKLKQRLQELISEEQ 214
           RD I+ KD  A       P   V+ + P  P+VVFINP SGGRHG  LK+RLQ+L+SEEQ
Sbjct: 61  RDCIKRKDSSAIPDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQ 120

Query: 215 VFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELY 274
           VFDL +VKPHEFV+YGL CLE +A  GD CAK+ R ++RIMVAGGDGTVGWVLG L EL 
Sbjct: 121 VFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELN 180

Query: 275 KQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVS 334
           K  ++ + PVG+IPLGTGNDL+RSFGWGGSFPFAW+SAVKR+L RA+ G V  LDSW + 
Sbjct: 181 KDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKIL 240

Query: 335 LSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVA 394
           +SMP+GE V+PP+SLK  EE++L++    LD   D+P     +EGVFYNY SIGMDAQVA
Sbjct: 241 VSMPSGEVVDPPYSLKPAEENELDQG---LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVA 300

Query: 395 YGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINC 454
           YGFHHLRN +PYLAQGP++NK+IYSS+ C+QGWF T C N+P L GL+NI++IHIKK+NC
Sbjct: 301 YGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNC 360

Query: 455 AEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQ 514
           ++WE++ VP +VRSIVALNLH+Y SG +PWG LKP+YLEKRGFVEAH DDGL+EIFG KQ
Sbjct: 361 SQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQ 420

Query: 515 GWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKR 574
           GWH SFVM ++ISAKHIAQAAA+R E+RGG  + A+LQMDGEPWKQP+S E STFVEIK+
Sbjct: 421 GWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKK 480

Query: 575 VPFQSLMINGD 580
           VP+QSLMIN +
Sbjct: 481 VPYQSLMINNE 488

BLAST of Cucsat.G20248.T1 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.5e-181
Identity = 304/487 (62.42%), Postives = 378/487 (77.62%), Query Frame = 0

Query: 102 PGAGDEVT--VAARSSFIDSLKG------CGLSGIRIDKHELNTKVIMPQYLRFALRDSI 161
           P  GD +T  +  R S +DS           L+ IR+ K EL  +V++PQYLR A+RD I
Sbjct: 4   PSIGDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIAIRDCI 63

Query: 162 -RLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 221
            R  D    +S+  PP    +  PE PL+VF+NP+SGGR G  +K+RLQ LISEEQV+DL
Sbjct: 64  LRKDDSFDASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDL 123

Query: 222 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 281
            +VKP+EF++YGL CLE  A  GD CAK+ R+K+RI+VAGGDGTVGWVLG L EL  Q+R
Sbjct: 124 TEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLGELNLQNR 183

Query: 282 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 341
            PV PV I+PLGTGNDL+RSFGWGGSFPFAWKSA+KR+L RA+   +  LDSW++ ++MP
Sbjct: 184 LPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSWNILITMP 243

Query: 342 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 401
           +GE V+PP+SLK T+E  +++    L+   + P     +EGVFYNYFSIGMDAQVAYGFH
Sbjct: 244 SGEIVDPPYSLKATQECYIDQ---NLEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFH 303

Query: 402 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 461
           HLRN++PYLA GP+ NK+IYS Y C+QGWF T C N+P L GLKNI+ +HIKK++ +EWE
Sbjct: 304 HLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWE 363

Query: 462 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 521
           KVPVP SVR++VALNLH+Y SGRNPWG LK +YLEKRGFVEA ADDGLLEIFGLKQGWH 
Sbjct: 364 KVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHA 423

Query: 522 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 580
           SFVMV++ISAKHIAQAAAIR+EIRGG  K A++QMDGEPWKQP++++ STFV+IKRVP Q
Sbjct: 424 SFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDYSTFVDIKRVPHQ 483

BLAST of Cucsat.G20248.T1 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 1.1e-70
Identity = 149/406 (36.70%), Postives = 225/406 (55.42%), Query Frame = 0

Query: 169 QNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLRC- 228
           Q +V   P  P+VVFIN RSGG+ G  L +  +EL+++ QVFDL +  P E V + L C 
Sbjct: 35  QVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCN 94

Query: 229 LEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTGN 288
            E L  +GD  A Q +  +R++VAGGDGT  W+LG +++L K    P  P+  +PLGTGN
Sbjct: 95  FEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-KLSHPP--PIATVPLGTGN 154

Query: 289 DLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAGEK--------VEP 348
           +L  SFGWG   P   + AVK  L +    +  ++DSWH+ + M A ++        +E 
Sbjct: 155 NLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQEGPCEPIAPLEL 214

Query: 349 PHSLKLTEESDLNEAVHKLDDIED-SPTKLTCFEGVFYNYFSIGMDAQVAYGFHHLRNKR 408
           PHSL          A H++   +  +      + G F+NYFS+GMDAQV+Y FH  R + 
Sbjct: 215 PHSL---------HAFHRVSGSDSLNMEGYHTYRGGFWNYFSMGMDAQVSYEFHSERKRN 274

Query: 409 PYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKVPVPS 468
           P   +  +TN+  Y+     QGWF  S + +P+   +  + ++ I K    +WE++ +P 
Sbjct: 275 PEKFKNQLTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQLAKVRIMKRPGGQWEELKIPR 334

Query: 469 SVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVD 528
           S+RSIV LNL +++ G NPWG      ++ R       DDGL+E+ G +  WH   ++  
Sbjct: 335 SIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAP 394

Query: 529 IISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFV 565
                 +AQA  IR E   G  +  ++++DGEPWKQP+ K++ T V
Sbjct: 395 NGHGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDDDTVV 426

BLAST of Cucsat.G20248.T1 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.0e-69
Identity = 152/417 (36.45%), Postives = 228/417 (54.68%), Query Frame = 0

Query: 157 DPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVK 216
           +P A  S TT P+N        P++VFIN +SGG+ G +L    + L++++QVFDL    
Sbjct: 33  EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVET 92

Query: 217 PHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVL 276
           P + +Q     LE L D  D  A + R K++I+VAGGDGT GW+LG +++L     NP  
Sbjct: 93  PDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLS--NPP- 152

Query: 277 PVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAGEK 336
           P+  +PLGTGN+L  +FGWG   P   +S+V+  L +    +   +D+W + + M   ++
Sbjct: 153 PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKE 212

Query: 337 ------VEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYG 396
                 ++ PHSL     SD           +++      + G F+NYFS+GMDAQV+Y 
Sbjct: 213 GSCDITLKLPHSLPRIFPSD-----------QENMEGYHTYRGGFWNYFSLGMDAQVSYA 272

Query: 397 FHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAE 456
           FH  R   P   +  + N+  Y   SCTQGWFF S   +P+   +  + +I I   N  +
Sbjct: 273 FHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQ 332

Query: 457 WEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGW 516
           W  + +P S+RSIV LNL +++ G NPWG   P+    R       DDGL+EI G +  W
Sbjct: 333 WNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAW 392

Query: 517 HTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI-SKENSTFVEI 567
           H   ++        +AQA  +R+E + G  K AY+++DGEPWKQP+ S + +  VEI
Sbjct: 393 HGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of Cucsat.G20248.T1 vs. NCBI nr
Match: XP_011656147.1 (diacylglycerol kinase 7 [Cucumis sativus] >KAE8653068.1 hypothetical protein Csa_019575 [Cucumis sativus])

HSP 1 Score: 985 bits (2546), Expect = 0.0
Identity = 484/485 (99.79%), Postives = 484/485 (99.79%), Query Frame = 0

Query: 95  MDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 154
           MDHSSA PGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR
Sbjct: 1   MDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 60

Query: 155 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 214
           LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD
Sbjct: 61  LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 120

Query: 215 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 274
           VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP
Sbjct: 121 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 180

Query: 275 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 334
           VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG
Sbjct: 181 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 240

Query: 335 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 394
           EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL
Sbjct: 241 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 300

Query: 395 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 454
           RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV
Sbjct: 301 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 360

Query: 455 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 514
           PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF
Sbjct: 361 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 420

Query: 515 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 574
           VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL
Sbjct: 421 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 480

Query: 575 MINGD 579
           MINGD
Sbjct: 481 MINGD 485

BLAST of Cucsat.G20248.T1 vs. NCBI nr
Match: XP_008453656.1 (PREDICTED: diacylglycerol kinase 7 [Cucumis melo] >KAA0059727.1 diacylglycerol kinase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 476/487 (97.74%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 95  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDLARSFGWGGSFPF WKSAVKRSLLRATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGWGGSFPFVWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD IEDSPTKL CFEGVFYNYFSIGMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGIEDSPTKLACFEGVFYNYFSIGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMING+
Sbjct: 481 SLMINGE 487

BLAST of Cucsat.G20248.T1 vs. NCBI nr
Match: TYK09464.1 (diacylglycerol kinase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 475/487 (97.54%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 95  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDLARSFGWGGSFPF WKSAVKRSLLRATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGWGGSFPFVWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD IEDSPTKL CFEGVFYNYFS+GMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGIEDSPTKLACFEGVFYNYFSVGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMING+
Sbjct: 481 SLMINGE 487

BLAST of Cucsat.G20248.T1 vs. NCBI nr
Match: XP_038878332.1 (diacylglycerol kinase 7-like isoform X1 [Benincasa hispida])

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 451/487 (92.61%), Postives = 463/487 (95.07%), Query Frame = 0

Query: 95  MDHSSAEPG--AGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHS A PG  AGD+VTVAARSS IDSLKGCGLSGIRIDK ELN KV+MP YLRFALRDS
Sbjct: 1   MDHSRAGPGVGAGDQVTVAARSSLIDSLKGCGLSGIRIDKQELNAKVMMPHYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQN+V   PE PLV+FINPRSGG HGQKLK RLQELIS+EQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNVVPSPPEAPLVIFINPRSGGHHGQKLKLRLQELISDEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           L+V P EFVQYGLRCLEILADHGD CAKQTRQKIRIMVAGGDGTVGWVLG +AELYKQDR
Sbjct: 121 LEVNPREFVQYGLRCLEILADHGDACAKQTRQKIRIMVAGGDGTVGWVLGCIAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDL+RSFGWGGSFPFAWKSAVKRSL RATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD  ED PTKL C+EGVFYNYFSIGMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGKEDLPTKLACYEGVFYNYFSIGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSN+PNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNDPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKE STFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKEFSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMINGD
Sbjct: 481 SLMINGD 487

BLAST of Cucsat.G20248.T1 vs. NCBI nr
Match: XP_038878333.1 (diacylglycerol kinase 7-like isoform X2 [Benincasa hispida])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 440/487 (90.35%), Postives = 451/487 (92.61%), Query Frame = 0

Query: 95  MDHSSAEPG--AGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHS A PG  AGD+VTVAARSS IDSLKGCGLSGIRIDK ELN KV+MP YLRFALRDS
Sbjct: 1   MDHSRAGPGVGAGDQVTVAARSSLIDSLKGCGLSGIRIDKQELNAKVMMPHYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQN+V   PE PLV+FINPRSGG HGQKLK RLQELIS+EQ    
Sbjct: 61  IRLKDPIAGASATTPPQNVVPSPPEAPLVIFINPRSGGHHGQKLKLRLQELISDEQ---- 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
                     YGLRCLEILADHGD CAKQTRQKIRIMVAGGDGTVGWVLG +AELYKQDR
Sbjct: 121 ----------YGLRCLEILADHGDACAKQTRQKIRIMVAGGDGTVGWVLGCIAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDL+RSFGWGGSFPFAWKSAVKRSL RATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD  ED PTKL C+EGVFYNYFSIGMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGKEDLPTKLACYEGVFYNYFSIGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSN+PNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNDPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKE STFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKEFSTFVEIKRVPFQ 473

Query: 575 SLMINGD 579
           SLMINGD
Sbjct: 481 SLMINGD 473

BLAST of Cucsat.G20248.T1 vs. ExPASy TrEMBL
Match: A0A0A0LZ78 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1)

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 559/560 (99.82%), Postives = 559/560 (99.82%), Query Frame = 0

Query: 20  SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP 79
           SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP
Sbjct: 30  SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP 89

Query: 80  QQQNTNLPRFSNSPQMDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV 139
           QQQNTNLPRFSNSPQMDHSSA PGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV
Sbjct: 90  QQQNTNLPRFSNSPQMDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV 149

Query: 140 IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR 199
           IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR
Sbjct: 150 IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR 209

Query: 200 LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW 259
           LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW
Sbjct: 210 LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW 269

Query: 260 VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQV 319
           VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQV
Sbjct: 270 VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQV 329

Query: 320 GSLDSWHVSLSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYF 379
           GSLDSWHVSLSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYF
Sbjct: 330 GSLDSWHVSLSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYF 389

Query: 380 SIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNII 439
           SIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNII
Sbjct: 390 SIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNII 449

Query: 440 RIHIKKINCAEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDG 499
           RIHIKKINCAEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDG
Sbjct: 450 RIHIKKINCAEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDG 509

Query: 500 LLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKE 559
           LLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKE
Sbjct: 510 LLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKE 569

Query: 560 NSTFVEIKRVPFQSLMINGD 579
           NSTFVEIKRVPFQSLMINGD
Sbjct: 570 NSTFVEIKRVPFQSLMINGD 589

BLAST of Cucsat.G20248.T1 vs. ExPASy TrEMBL
Match: A0A5A7UYK3 (diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold551G00030 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 476/487 (97.74%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 95  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDLARSFGWGGSFPF WKSAVKRSLLRATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGWGGSFPFVWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD IEDSPTKL CFEGVFYNYFSIGMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGIEDSPTKLACFEGVFYNYFSIGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMING+
Sbjct: 481 SLMINGE 487

BLAST of Cucsat.G20248.T1 vs. ExPASy TrEMBL
Match: A0A1S3BXZ0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103494309 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 476/487 (97.74%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 95  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDLARSFGWGGSFPF WKSAVKRSLLRATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGWGGSFPFVWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD IEDSPTKL CFEGVFYNYFSIGMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGIEDSPTKLACFEGVFYNYFSIGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMING+
Sbjct: 481 SLMINGE 487

BLAST of Cucsat.G20248.T1 vs. ExPASy TrEMBL
Match: A0A5D3CE44 (diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00590 PE=3 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 475/487 (97.54%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 95  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 154
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 155 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 214
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 215 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 274
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 275 NPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 334
           NPVLPVGIIPLGTGNDLARSFGWGGSFPF WKSAVKRSLLRATTGQVGSLDSWHVSLSMP
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGWGGSFPFVWKSAVKRSLLRATTGQVGSLDSWHVSLSMP 240

Query: 335 AGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFH 394
           AGEKVEPPHSLKLTEESD+NEAVHKLD IEDSPTKL CFEGVFYNYFS+GMDAQVAYGFH
Sbjct: 241 AGEKVEPPHSLKLTEESDMNEAVHKLDGIEDSPTKLACFEGVFYNYFSVGMDAQVAYGFH 300

Query: 395 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWE 454
           HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNI+RIHIKKINCAEWE
Sbjct: 301 HLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNILRIHIKKINCAEWE 360

Query: 455 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 514
           KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT
Sbjct: 361 KVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHT 420

Query: 515 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 574
           SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ
Sbjct: 421 SFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQ 480

Query: 575 SLMINGD 579
           SLMING+
Sbjct: 481 SLMINGE 487

BLAST of Cucsat.G20248.T1 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 427/485 (88.04%), Postives = 454/485 (93.61%), Query Frame = 0

Query: 95  MDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 154
           MDHS      GD+V++AARSS  DSLKGCGLSGI IDK ELNTKV+MPQYLR ALRDSIR
Sbjct: 1   MDHSR---DGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIR 60

Query: 155 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 214
           LKDP  GASA+ P QN+V PLPE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLL+
Sbjct: 61  LKDPTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLE 120

Query: 215 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 274
           VKPHEFVQYGLRCLEILAD GD+CAKQTRQ++R+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 121 VKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKP 180

Query: 275 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 334
           VLPVGIIPLGTGNDL+RSFGWGGSFPFAW+SAVKRSL RATTGQVGSLDSWHV LS+PAG
Sbjct: 181 VLPVGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAG 240

Query: 335 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 394
           EKVEPPHSLKLTEESDLNEAV KLD+  D PTKL  +EGVFYNYFSIGMDAQVAYGFHHL
Sbjct: 241 EKVEPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHL 300

Query: 395 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 454
           RNKRPYLAQGP+TNKLIYSSYSCTQGWFFTSCSN+PNL GLKNI+RIHIKKINC+EWEK+
Sbjct: 301 RNKRPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKI 360

Query: 455 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 514
           P+PSSVRSIV LNLHNYASGRNPWGK KP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSF
Sbjct: 361 PIPSSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 420

Query: 515 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 574
           VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+K+ ST+VEIKRVPFQSL
Sbjct: 421 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSL 480

Query: 575 MINGD 579
           MINGD
Sbjct: 481 MINGD 482

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JQ952.3e-18766.24Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1[more]
Q8VZG19.9e-18663.34Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1[more]
Q1PDI21.5e-18162.42Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1[more]
Q7XQT21.1e-7036.70Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV... [more]
F4JKI32.0e-6936.45Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011656147.10.099.79diacylglycerol kinase 7 [Cucumis sativus] >KAE8653068.1 hypothetical protein Csa... [more]
XP_008453656.10.097.74PREDICTED: diacylglycerol kinase 7 [Cucumis melo] >KAA0059727.1 diacylglycerol k... [more]
TYK09464.10.097.54diacylglycerol kinase 7 [Cucumis melo var. makuwa][more]
XP_038878332.10.092.61diacylglycerol kinase 7-like isoform X1 [Benincasa hispida][more]
XP_038878333.10.090.35diacylglycerol kinase 7-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LZ780.099.82Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1[more]
A0A5A7UYK30.097.74diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55... [more]
A0A1S3BXZ00.097.74diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103494309 PE=3 SV=1[more]
A0A5D3CE440.097.54diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49... [more]
A0A6J1E1C60.088.04diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 179..325
e-value: 2.6E-28
score: 110.1
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 179..319
e-value: 1.4E-30
score: 105.5
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 175..332
score: 30.054396
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 374..551
e-value: 9.5E-9
score: 27.9
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 374..551
e-value: 2.3E-42
score: 145.0
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 177..324
e-value: 3.2E-23
score: 83.7
NoneNo IPR availableGENE3D2.60.200.40coord: 372..559
e-value: 8.0E-7
score: 31.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..98
NoneNo IPR availablePANTHERPTHR11255:SF96DIACYLGLYCEROL KINASEcoord: 104..578
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 104..578
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 179..553

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G20248Cucsat.G20248gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20248.T1.E1Cucsat.G20248.T1.E1exon
Cucsat.G20248.T1.E2Cucsat.G20248.T1.E2exon
Cucsat.G20248.T1.E3Cucsat.G20248.T1.E3exon
Cucsat.G20248.T1.E4Cucsat.G20248.T1.E4exon
Cucsat.G20248.T1.E5Cucsat.G20248.T1.E5exon
Cucsat.G20248.T1.E6Cucsat.G20248.T1.E6exon
Cucsat.G20248.T1.E7Cucsat.G20248.T1.E7exon
Cucsat.G20248.T1.E8Cucsat.G20248.T1.E8exon
Cucsat.G20248.T1.E9Cucsat.G20248.T1.E9exon
Cucsat.G20248.T1.E10Cucsat.G20248.T1.E10exon
Cucsat.G20248.T1.E11Cucsat.G20248.T1.E11exon
Cucsat.G20248.T1.E12Cucsat.G20248.T1.E12exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20248.T1.C12Cucsat.G20248.T1.C12CDS
Cucsat.G20248.T1.C11Cucsat.G20248.T1.C11CDS
Cucsat.G20248.T1.C10Cucsat.G20248.T1.C10CDS
Cucsat.G20248.T1.C9Cucsat.G20248.T1.C9CDS
Cucsat.G20248.T1.C8Cucsat.G20248.T1.C8CDS
Cucsat.G20248.T1.C7Cucsat.G20248.T1.C7CDS
Cucsat.G20248.T1.C6Cucsat.G20248.T1.C6CDS
Cucsat.G20248.T1.C5Cucsat.G20248.T1.C5CDS
Cucsat.G20248.T1.C4Cucsat.G20248.T1.C4CDS
Cucsat.G20248.T1.C3Cucsat.G20248.T1.C3CDS
Cucsat.G20248.T1.C2Cucsat.G20248.T1.C2CDS
Cucsat.G20248.T1.C1Cucsat.G20248.T1.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G20248.T1Cucsat.G20248.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
biological_process GO:0006952 defense response
biological_process GO:0016310 phosphorylation
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0005524 ATP binding