Cucsat.G20248 (gene) Cucumber (B10) v3

Overview
NameCucsat.G20248
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDiacylglycerol kinase
Locationctg47: 281224 .. 284615 (-)
RNA-Seq ExpressionCucsat.G20248
SyntenyCucsat.G20248
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATCTAAAAAAAGGAGGATTGAAGGAAGCTCAATGTTTTTCGAATTTTTGTGTAAAGTCAGAATATTTAGTTGACATCAGACGTAAAGATGAGGAATTGTCTGACATCGGCCAGCGCAGTCCCTGTGATGCCAAACCAAAGTCCCCATCACCGACGCCACTCTGTTTACTCCTCACTCACAACCTTCCAACCTCCCGCTTTTATTGTTCCTACTCACCTAAGCTTTACTTCACCCCACAACAACAAAACACCAATCTTCCCCGCTTCTCAAACTCCCCACAAATGGACCATTCAAGCGCCGAACCCGGTGCCGGCGACGAAGTTACTGTAGCAGCTCGCTCCTCCTTCATCGACTCTCTCAAAGGCTGCGGCCTCTCCGGTATCCGAATCGATAAACATGAGCTCAATACAAAGGTTATAATGCCCCAATATCTCCGCTTTGCCTTGCGTGATTCTATCCGCTTGAAAGACCCCATTGCCGGAGCCTCTGCCACTACTCCGCCGCAGAATATGGTATCGCCATTGCCGGAGACCCCTTTGGTGGTCTTCATTAACCCACGTAGCGGTGGCCGCCATGGCCAGAAGCTTAAGCAGCGACTCCAGGAATTAATCAGCGAAGAACAGGTCCACTATTTATTCTGTGAAGAAGTCTCGGGATTCCCATTGTTTTTTCTTCTTTTTACGTGTGTCATCTTTGGAATTGCTGTACTTGTTTTTTGTTTGATTCTTATTTTTGGTGGGTGTAATTGTTGTATTTGAATTTCGTATCTGATTTTTTGTATGTATAATTGAGTGAGGATTTACTTCAGAGTTTGTATTGTACTGTTGTAGGTGTTTGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTATGGACTGCGTTGCCTGGAAATCTTGGCTGATCATGGCGATGTTTGTGCTAAACAAACTCGTCAAAAAATAAGGATCATGGTATTTTGATATTCACACTAACCCCACATAAAAAGATTGTTTCTATTGCTTTACATATTTGTTATCCAATGGAACTGTTTCGTATTGGAAAATTGAGGAAGAGAACTTGAACTGGTGGAGCAGGTTGCAGGAGGTGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGATCGCAACCCTGTTCTTCCTGTTGGGATTATTCCTCTTGGTACTGGGAATGATCTTGCCAGAAGTTTTGGTTGGGTGAGTTTTTGTAGTTTCAACTCGAAATTTTATAGGAAGGCATTATTTTTTCCTTTTCTACTCCAATTCAGAATCATTGCTTTGTTTTTGTTTTTTTTTTTAATCAGGGAGGTTCATTTCCTTTTGCTTGGAAATCTGCTGTTAAAAGATCTCTCCTCAGGGCTACCACTGGTCAAGTTGGTTCTTTGGATAGGTGAGAATTTGATTTTTTTTCTGAACTTCGAAGCTGTTTCTGGAGTTGGTTTTAAATTTGAATTTCTCTTGATGCAGTTGGCATGTTTCATTGTCGATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTTTGAAGCTAACAGAAGAAAGTGATCTGAATGAAGCTGTCCATGTAATTATATACTGTTTCAATTATACTATACCAGTTCCAATCACTTATCCACACAAAATAGAAGAAAATGTTGGAATTTATTTCTCAGTTTGTGATTGGAAATGTTGTCCCATGGCTGCAGAAACTGGATGACATAGAAGATTCACCTACCAAACTGACTTGTTTTGAAGGAGTATTTTATAATTACTTCAGCATAGGTAAGTTGAACTCACTGGTGAAGTTAAACTCTGAAATGTATATGTTTAGAGAAGTGATTAGTTGTAATTTCACTAGCAAAGTTGATTGTGTCTTGAACTTAATAGTCCCATGGAGGATTAGATTAATTAGTCATCATTCTGCTTCTCTTCTTTAAGACGATTGTCTATTTGCTTATCTGCAAAATCATTAGCAGTTGTTAATTAACCAATTCTATTAATGTACAATGCAGGAATGGATGCTCAAGTTGCCTACGGCTTCCACCATTTGCGCAACAAAAGACCGTACTTGGCTCAAGGTCCAGTTACAAATAAGGTTGAACTGACTATTATATTTATCCGTTAAACAAAACATGATTTCTTTTTTGATTAGTGGTTGTTTATAAATCTTTTATTTCTGACAGTTAATTTACTCAAGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAACAATCCAAATTTGAGGTGATAGCTTATACTTATTAAAAACCCAGTAGAATGCATATATATTCTCAGTTTCTAACCACATAAAGCTTTTCTTGTTGTAGTGGGCTTAAGAACATCATTAGAATACATATAAAAAAGATCAACTGTGCAGAATGGGAAAAGGTTCCAGTTCCTTCCAGGTAGAACTGCTCTCTCTACTCTCTTATGTGCGAATCGTGAAACTTGTTTACTTCTTTTTTGGGGGACATCAATTGATGTTGTTTTTTTGGTTGGGCTATAGTGTTAGGTCTATTGTTGCTTTAAACTTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCTAAAGCCAGAGTACTTGGAGAAGGTACTGACGACGAAGTTCTGTTATGCTATTGTGTTTTTTTAGTTAGCATTGTTGTTTTCTCTTCATGCTATACATTGACTGAAATATTTATTTTGTACTAGAGAGGTTTTGTGGAAGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTAAAACAAGGATGGCATACATCATTTGTAATGGTAGATATAATCTCTGCCAAACATATCGCCCAGGTGCTTCATCTTTTTCAGGAAAATTACTACTTGGTTTTCTACTCCAAACACGATTTGTGTTTTTGAACTGTTGAATGTTTATTTACAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGAAAGAAGGCCTATCTGCAAATGGATGGGGAGCCATGGAAACAACCAATAAGCAAGGAAAACTCGACGTTTGTTGAAATCAAGAGGGTCCCTTTTCAATCACTAATGATCAATGGGGACTAAAAGCTTCTTCATCTCTCATCATCTCCTCTTCAGTTTGCTGGCCGAGTTGATATATTCTTTTTTGTATGATATTATCATATATATGTATGTCTAAATTATTCATGTTCAACACAACCCTTAGAACATCCCTGAGGGCCTGAGGCTTAAAAGTATGTCTAGTAGGCAATCTGAAAATGGGAAGGTGAAAGTAAGAGATTTACTTATAACAGGGTTATATTTCATGTTTTTATACCTGTGTAAAGAAACTTAGGTCTCAATCAAAGTTATAATGCTTTTAAATTTGTACCAATTACTTTAGTAAGCAATAGTTCAGTCCTTAAATCTTCAATTTGTGACAATTTCGGTCCTTGTTTTAGAGTATGACAATATTGTTTAGA

Coding sequence (CDS)

TATCTAAAAAAAGGAGGATTGAAGGAAGCTCAATGTTTTTCGAATTTTTGTGTAAAGTCAGAATATTTAGTTGACATCAGACGTAAAGATGAGGAATTGTCTGACATCGGCCAGCGCAGTCCCTGTGATGCCAAACCAAAGTCCCCATCACCGACGCCACTCTGTTTACTCCTCACTCACAACCTTCCAACCTCCCGCTTTTATTGTTCCTACTCACCTAAGCTTTACTTCACCCCACAACAACAAAACACCAATCTTCCCCGCTTCTCAAACTCCCCACAAATGGACCATTCAAGCGCCGAACCCGGTGCCGGCGACGAAGTTACTGTAGCAGCTCGCTCCTCCTTCATCGACTCTCTCAAAGGCTGCGGCCTCTCCGGTATCCGAATCGATAAACATGAGCTCAATACAAAGGTTATAATGCCCCAATATCTCCGCTTTGCCTTGCGTGATTCTATCCGCTTGAAAGACCCCATTGCCGGAGCCTCTGCCACTACTCCGCCGCAGAATATGGTATCGCCATTGCCGGAGACCCCTTTGGTGGTCTTCATTAACCCACGTAGCGGTGGCCGCCATGGCCAGAAGCTTAAGCAGCGACTCCAGGAATTAATCAGCGAAGAACAGGTGTTTGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTATGGACTGCGTTGCCTGGAAATCTTGGCTGATCATGGCGATGTTTGTGCTAAACAAACTCGTCAAAAAATAAGGATCATGGTTGCAGGAGGTGATGGTACTGTCGGTTGGGTACTGGGAAGTCTAGCAGAACTTTACAAACAAGATCGCAACCCTGTTCTTCCTGTTGGGATTATTCCTCTTGGTACTGGGAATGATCTTGCCAGAAGTTTTGGTTGGGGAGGTTCATTTCCTTTTGCTTGGAAATCTGCTGTTAAAAGATCTCTCCTCAGGGCTACCACTGGTCAAGTTGGTTCTTTGGATAGTTGGCATGTTTCATTGTCGATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTTTGAAGCTAACAGAAGAAAGTGATCTGAATGAAGCTGTCCATAAACTGGATGACATAGAAGATTCACCTACCAAACTGACTTGTTTTGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCCTACGGCTTCCACCATTTGCGCAACAAAAGACCGTACTTGGCTCAAGGTCCAGTTACAAATAAGTTAATTTACTCAAGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAACAATCCAAATTTGAGTGGGCTTAAGAACATCATTAGAATACATATAAAAAAGATCAACTGTGCAGAATGGGAAAAGGTTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACTTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCTAAAGCCAGAGTACTTGGAGAAGAGAGGTTTTGTGGAAGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGCCTAAAACAAGGATGGCATACATCATTTGTAATGGTAGATATAATCTCTGCCAAACATATCGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGAAAGAAGGCCTATCTGCAAATGGATGGGGAGCCATGGAAACAACCAATAAGCAAGGAAAACTCGACGTTTGTTGAAATCAAGAGGGTCCCTTTTCAATCACTAATGATCAATGGGGACTAA

Protein sequence

YLKKGGLKEAQCFSNFCVKSEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTPQQQNTNLPRFSNSPQMDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAGEKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKVPVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSLMINGD
Homology
[Close]
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 4.6e-59
Identity = 117/207 (56.52%), Postives = 148/207 (71.50%), Query Frame = 0 

Query: 92  MDHSSAEPGAGDEVTVAARSSFIDS--LKGCGLSG---IRIDKHELNTKVIMPQYLRFAL 151
           MD   ++  A  E  VA+R S  DS  ++GCGL+    + +DK EL  +++MP+YLR A+
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 152 RDSIRLKDPIAGASATTPPQNMVSPL-PETPLVVFINPRSGGRHGQKLKQRLQELISEEQ 211
           RD I+ KD  A       P   V+ + P  P+VVFINP SGGRHG  LK+RLQ+L+SEEQ
Sbjct: 61  RDCIKRKDSSAIPDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQ 120

Query: 212 VFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELY 271
           VFDL +VKPHEFV+YGL CLE +A  GD CAK+ R ++RIMVAGGDGTVGWVLG L EL 
Sbjct: 121 VFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELN 180

Query: 272 KQDRNPVLPVGIIPLGTGNDLARSFGW 293
           K  ++ + PVG+IPLGTGNDL+RSFGW
Sbjct: 181 KDGKSQIPPVGVIPLGTGNDLSRSFGW 207

[Close]
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.1e-56
Identity = 112/203 (55.17%), Postives = 141/203 (69.46%), Query Frame = 0 

Query: 99  PGAGDEVT--VAARSSFIDSLKG------CGLSGIRIDKHELNTKVIMPQYLRFALRDSI 158
           P  GD +T  +  R S +DS           L+ IR+ K EL  +V++PQYLR A+RD I
Sbjct: 4   PSIGDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIAIRDCI 63

Query: 159 -RLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 218
            R  D    +S+  PP    +  PE PL+VF+NP+SGGR G  +K+RLQ LISEEQV+DL
Sbjct: 64  LRKDDSFDASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDL 123

Query: 219 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 278
            +VKP+EF++YGL CLE  A  GD CAK+ R+K+RI+VAGGDGTVGWVLG L EL  Q+R
Sbjct: 124 TEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLGELNLQNR 183

Query: 279 NPVLPVGIIPLGTGNDLARSFGW 293
            PV PV I+PLGTGNDL+RSFGW
Sbjct: 184 LPVPPVSIMPLGTGNDLSRSFGW 206

[Close]
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.6e-56
Identity = 106/189 (56.08%), Postives = 140/189 (74.07%), Query Frame = 0 

Query: 108 AARSSFIDSLKGCG---LSGIRIDKHELNTKVIMPQYLRFALRDSIRLKDPIAGASATTP 167
           +A++    +++GCG   L+ + IDK EL  ++ MP+YLR A+RD I+ KD    +     
Sbjct: 22  SAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLL 81

Query: 168 PQNMVSPL-PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLR 227
           P    + + P  P+VVFINP+SGGRHG  LK+RLQ+L++EEQVFDL +VKPHEFV+YGL 
Sbjct: 82  PGGAAADMAPHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLG 141

Query: 228 CLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTG 287
           CL+ LA  GD CA++ R+KIRIMVAGGDGTVGWVLG L EL+K  ++ + PVG+IPLGTG
Sbjct: 142 CLDTLAAKGDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTG 201

Query: 288 NDLARSFGW 293
           NDL+RSF W
Sbjct: 202 NDLSRSFSW 210

[Close]
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 59/128 (46.09%), Postives = 81/128 (63.28%), Query Frame = 0 

Query: 166 QNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVKPHEFVQYGLRC- 225
           Q +V   P  P+VVFIN RSGG+ G  L +  +EL+++ QVFDL +  P E V + L C 
Sbjct: 35  QVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCN 94

Query: 226 LEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVLPVGIIPLGTGN 285
            E L  +GD  A Q +  +R++VAGGDGT  W+LG +++L K    P  P+  +PLGTGN
Sbjct: 95  FEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-KLSHPP--PIATVPLGTGN 154

Query: 286 DLARSFGW 293
           +L  SFGW
Sbjct: 155 NLPFSFGW 158

[Close]
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-20
Identity = 59/139 (42.45%), Postives = 85/139 (61.15%), Query Frame = 0 

Query: 154 DPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLDVK 213
           +P A  S TT P+N        P++VFIN +SGG+ G +L    + L++++QVFDL    
Sbjct: 33  EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVET 92

Query: 214 PHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNPVL 273
           P + +Q     LE L D  D  A + R K++I+VAGGDGT GW+LG +++L     NP  
Sbjct: 93  PDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLS--NPP- 152

Query: 274 PVGIIPLGTGNDLARSFGW 293
           P+  +PLGTGN+L  +FGW
Sbjct: 153 PIATVPLGTGNNLPFAFGW 158

[Close]
BLAST of Cucsat.G20248 vs. NCBI nr
Match: XP_011656147.1 (diacylglycerol kinase 7 [Cucumis sativus] >KAE8653068.1 hypothetical protein Csa_019575 [Cucumis sativus])

HSP 1 Score: 404 bits (1038), Expect = 6.50e-135
Identity = 200/201 (99.50%), Postives = 200/201 (99.50%), Query Frame = 0 

Query: 92  MDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 151
           MDHSSA PGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR
Sbjct: 1   MDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 60

Query: 152 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 211
           LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD
Sbjct: 61  LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 120

Query: 212 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 271
           VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP
Sbjct: 121 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 180

Query: 272 VLPVGIIPLGTGNDLARSFGW 292
           VLPVGIIPLGTGNDLARSFGW
Sbjct: 181 VLPVGIIPLGTGNDLARSFGW 201

[Close]
BLAST of Cucsat.G20248 vs. NCBI nr
Match: XP_008453656.1 (PREDICTED: diacylglycerol kinase 7 [Cucumis melo] >KAA0059727.1 diacylglycerol kinase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 394 bits (1012), Expect = 5.91e-131
Identity = 198/203 (97.54%), Postives = 198/203 (97.54%), Query Frame = 0 

Query: 92  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDLARSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. NCBI nr
Match: TYK09464.1 (diacylglycerol kinase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 394 bits (1012), Expect = 5.91e-131
Identity = 198/203 (97.54%), Postives = 198/203 (97.54%), Query Frame = 0 

Query: 92  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDLARSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. NCBI nr
Match: XP_038878332.1 (diacylglycerol kinase 7-like isoform X1 [Benincasa hispida])

HSP 1 Score: 356 bits (914), Expect = 3.70e-116
Identity = 177/203 (87.19%), Postives = 185/203 (91.13%), Query Frame = 0 

Query: 92  MDHSSAEPG--AGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHS A PG  AGD+VTVAARSS IDSLKGCGLSGIRIDK ELN KV+MP YLRFALRDS
Sbjct: 1   MDHSRAGPGVGAGDQVTVAARSSLIDSLKGCGLSGIRIDKQELNAKVMMPHYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQN+V   PE PLV+FINPRSGG HGQKLK RLQELIS+EQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNVVPSPPEAPLVIFINPRSGGHHGQKLKLRLQELISDEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           L+V P EFVQYGLRCLEILADHGD CAKQTRQKIRIMVAGGDGTVGWVLG +AELYKQDR
Sbjct: 121 LEVNPREFVQYGLRCLEILADHGDACAKQTRQKIRIMVAGGDGTVGWVLGCIAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDL+RSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLSRSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. NCBI nr
Match: KAG7023409.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348 bits (893), Expect = 1.47e-113
Identity = 170/201 (84.58%), Postives = 183/201 (91.04%), Query Frame = 0 

Query: 92  MDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 151
           MDHS      GD+V++AARSS  DSLKGCGLSGI IDK ELNTKV+MPQYLR ALRDSIR
Sbjct: 1   MDHSR---DGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIR 60

Query: 152 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 211
           LKDP  GASA+ PPQN+V PLPE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLL+
Sbjct: 61  LKDPTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLE 120

Query: 212 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 271
           VKPHEFVQYGLRCLEILAD GD+CAKQTRQ++R+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 121 VKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKP 180

Query: 272 VLPVGIIPLGTGNDLARSFGW 292
           VLPVGIIPLGTGNDL+RSFGW
Sbjct: 181 VLPVGIIPLGTGNDLSRSFGW 198

[Close]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match: A0A0A0LZ78 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 3.02e-195
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0 

Query: 17  SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP 76
           SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP
Sbjct: 30  SEYLVDIRRKDEELSDIGQRSPCDAKPKSPSPTPLCLLLTHNLPTSRFYCSYSPKLYFTP 89

Query: 77  QQQNTNLPRFSNSPQMDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV 136
           QQQNTNLPRFSNSPQMDHSSA PGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV
Sbjct: 90  QQQNTNLPRFSNSPQMDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKV 149

Query: 137 IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR 196
           IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR
Sbjct: 150 IMPQYLRFALRDSIRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQR 209

Query: 197 LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW 256
           LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW
Sbjct: 210 LQELISEEQVFDLLDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGW 269

Query: 257 VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGW 292
           VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGW
Sbjct: 270 VLGSLAELYKQDRNPVLPVGIIPLGTGNDLARSFGW 305

[Close]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match: A0A5D3CE44 (diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00590 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 2.86e-131
Identity = 198/203 (97.54%), Postives = 198/203 (97.54%), Query Frame = 0 

Query: 92  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDLARSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match: A0A5A7UYK3 (diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold551G00030 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 2.86e-131
Identity = 198/203 (97.54%), Postives = 198/203 (97.54%), Query Frame = 0 

Query: 92  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDLARSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match: A0A1S3BXZ0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103494309 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 2.86e-131
Identity = 198/203 (97.54%), Postives = 198/203 (97.54%), Query Frame = 0 

Query: 92  MDHSSAEPGA--GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 151
           MDHSSA PGA  GDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS
Sbjct: 1   MDHSSAGPGADTGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDS 60

Query: 152 IRLKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 211
           IRLKDPIAGASATTPPQNMVSP PETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL
Sbjct: 61  IRLKDPIAGASATTPPQNMVSPSPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDL 120

Query: 212 LDVKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 271
           LDVKPHEFVQYGLRCLEILADHGDVCAK TRQKIRIMVAGGDGTVGWVLGSLAELYKQDR
Sbjct: 121 LDVKPHEFVQYGLRCLEILADHGDVCAKLTRQKIRIMVAGGDGTVGWVLGSLAELYKQDR 180

Query: 272 NPVLPVGIIPLGTGNDLARSFGW 292
           NPVLPVGIIPLGTGNDLARSFGW
Sbjct: 181 NPVLPVGIIPLGTGNDLARSFGW 203

[Close]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 344 bits (883), Expect = 6.80e-112
Identity = 169/201 (84.08%), Postives = 182/201 (90.55%), Query Frame = 0 

Query: 92  MDHSSAEPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 151
           MDHS      GD+V++AARSS  DSLKGCGLSGI IDK ELNTKV+MPQYLR ALRDSIR
Sbjct: 1   MDHSR---DGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIR 60

Query: 152 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 211
           LKDP  GASA+ P QN+V PLPE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLL+
Sbjct: 61  LKDPTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLE 120

Query: 212 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 271
           VKPHEFVQYGLRCLEILAD GD+CAKQTRQ++R+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 121 VKPHEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKP 180

Query: 272 VLPVGIIPLGTGNDLARSFGW 292
           VLPVGIIPLGTGNDL+RSFGW
Sbjct: 181 VLPVGIIPLGTGNDLSRSFGW 198

The following BLAST results are available for this feature:
BLAST of Cucsat.G20248 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8VZG14.6e-5956.52Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1[more]
Q1PDI22.1e-5655.17Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1[more]
F4JQ953.6e-5656.08Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1[more]
Q7XQT21.7e-2146.09Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV... [more]
F4JKI31.9e-2042.45Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1[more]
BLAST of Cucsat.G20248 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011656147.16.50e-13599.50diacylglycerol kinase 7 [Cucumis sativus] >KAE8653068.1 hypothetical protein Csa... [more]
XP_008453656.15.91e-13197.54PREDICTED: diacylglycerol kinase 7 [Cucumis melo] >KAA0059727.1 diacylglycerol k... [more]
TYK09464.15.91e-13197.54diacylglycerol kinase 7 [Cucumis melo var. makuwa][more]
XP_038878332.13.70e-11687.19diacylglycerol kinase 7-like isoform X1 [Benincasa hispida][more]
KAG7023409.11.47e-11384.58Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Cucsat.G20248 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LZ783.02e-19599.64Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1[more]
A0A5D3CE442.86e-13197.54diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49... [more]
A0A5A7UYK32.86e-13197.54diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55... [more]
A0A1S3BXZ02.86e-13197.54diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103494309 PE=3 SV=1[more]
A0A6J1E4R56.80e-11284.08diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 179..325
e-value: 2.6E-28
score: 110.1
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 179..319
e-value: 1.4E-30
score: 105.5
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 175..332
score: 30.054396
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 374..551
e-value: 9.5E-9
score: 27.9
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 374..551
e-value: 2.3E-42
score: 145.0
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 177..324
e-value: 3.2E-23
score: 83.7
NoneNo IPR availableGENE3D2.60.200.40coord: 372..559
e-value: 8.0E-7
score: 31.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..98
NoneNo IPR availablePANTHERPTHR11255:SF96DIACYLGLYCEROL KINASEcoord: 104..578
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 104..578
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 179..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G20248.T1Cucsat.G20248.T1mRNA
Cucsat.G20248.T2Cucsat.G20248.T2mRNA
Cucsat.G20248.T3Cucsat.G20248.T3mRNA
Cucsat.G20248.T6Cucsat.G20248.T6mRNA
Cucsat.G20248.T14Cucsat.G20248.T14mRNA
Cucsat.G20248.T9Cucsat.G20248.T9mRNA
Cucsat.G20248.T7Cucsat.G20248.T7mRNA
Cucsat.G20248.T10Cucsat.G20248.T10mRNA
Cucsat.G20248.T12Cucsat.G20248.T12mRNA
Cucsat.G20248.T11Cucsat.G20248.T11mRNA
Cucsat.G20248.T4Cucsat.G20248.T4mRNA
Cucsat.G20248.T16Cucsat.G20248.T16mRNA
Cucsat.G20248.T5Cucsat.G20248.T5mRNA
Cucsat.G20248.T13Cucsat.G20248.T13mRNA
Cucsat.G20248.T8Cucsat.G20248.T8mRNA
Cucsat.G20248.T15Cucsat.G20248.T15mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0016310 phosphorylation
biological_process GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0016301 kinase activity