Cucsat.G2009.T10 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G2009.T10
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	ctg1002: 5450379 .. 5453318 (-)
RNA-Seq Expression	Cucsat.G2009.T10
Synteny	Cucsat.G2009.T10

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAACTTCTTCTTATCTCTGTTTCCCGTTCTGTCTTCTTCTCCTTCCTCAACCTTTTTCATTTCTACATTAACATTCCCTCTATTCTCTGCAACCAGTTTTTTCTCTCTATCTCTGTCCCAGGTTAACCACTGAATTTCAACTTTCATCAATACTTTTCTCTTTGTATTCTTGCTCCCTTTCCCTGTGATTGTGTTCGTTGAGGTGGAAGTTTTCACGCTTCTTTCCATTTGTTGATTAATCCATTTTTGTTCTTAATTTCTTACTATTTGTTTGCGATGTTTCATTGTAGCGATCGCGTTCTCAGATTTTCTGGTAGCATTTTTGTTTTCTGGATCTTTCATTCTGGATAAGTTTTCTGTTTATAGCTCTTTTTCTTCCCACTCTGTGTTTCTTTTTAATTTTTTAAAATTGGGAGTGTTGTATTTGGCTGTTGTATGATAATTTTATGTATGTGAGAATACACCAGTGGTTTCATTAGAAATTCTTTTGAATGTTCGGCCTACCGGTTGTACTGTGTATTTATGATTAGAGTTCTCATTCTATGGTTGGAAAGAGCAGGGTTTCTTATGGATCCTTGCGGCTTTGGTTGATGAATTTTCATCATTGACAGCTTTGTTAGGTAATCGACCACTTTTATCTGTTTCTGGTCGATGGAAAATAATCCGAGCAACACACACTGGGAGTAATTTATTTAGCCTTTGGGTCTGACGATTTGATTCAATTTGCTAACACGATCTGTTCTTATATATCTATCATAAAATTAAAAATTAGAACAGCAGAGATTTTTTTCACGTAGGAGAAAAGTTGTTCCCTCTTAATTCTGTATTTTTTTTTTTTTTTTTCGTGTGAAAGCATTTTGAAGCAGTGAGATATCCTCTTGTTTCTTGTCTGCTGTGTGCAGCATTTATCACATTATATGTCTAGAGTTCAGCAAATTCCTAACCATTTGATTTTGGGCTCTTTAAGGAAAACTAGAAGAGGATTTGGTTTTCTGGAGTTACCACTTGAAGTTCCATTTATGACTTTTTCTTGTGGATGGACCCCTAAATATTGAATGGGGAAGAGAAAGGGATAAGAGGTATCACGATTCTGGAGTGGGTATCTAATAAATGAGTACGTTTCTGCCTGCATATTGTGAATATGGGCACACTGAGGAGGACAATGTGCCAGGAATACACTTTTCTTCTTGGCAAACTAGCTAAACAATGATTTAACATTTAGGTCCTCTTCTTCTGTATTTATTCATTGGGCATATGAGTAAATAATGTAGTCATTTTCTTCAATCATTGGGTATGGGTATTGCTTGCTTTACATGTATTATTACTATGGTTAGCTCTAAGTTGACTGCTTTAATTGCTAATTCATTACAATAGCTGTTTTTTGCCATTGTTTCACCATTGCTATTCTATAGAATTTAAGAGCTAAACACATTCAGTTAATCAATTGAAAGCTCTTTGCATTTGAATTTGCCATGTGGCTGATTGATTTTATGGGGCTGTGAGTATTCTAGAATTTTACCCAACTCAATAATTTGGTTGATCTGTTTGCTATTTTATTGATATTACCCATCCTCTCTTACCTAGTAGTAGCTTGCTTCCTTCCATATCTGTGTTCATTTTGTGGTTGGTCTTGAGTAATTAAACTCTTGCAGGATGTGTTAATTAGGCATCTAACTACTCTGAAATAAAGTCGGTCACATGGTTGGTCACTTCCAATTGGGTTTTAGGTATCTTAAATGTCCCACCAACCTTCTATTTGGGTAGTCCGTAATGATCAATTGTTTTTAACTTTCTTCCCACAAACCATAAACTTAGCTTTAAATTCTGTTTTTAATTACTGTTTACCAATATCTTTTCAGATGGGAAAATTTTCCTTATAATTTGTTATCGTGTGCTTTACCAGGAAGCATCTGCAGTTTCTTGACAAATCCATGGCCTTTGAGCAGTACTTTGCGCAAGGATGGAAACATGTTTCTGGCACAGAGCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATTTGCTTAGACTTTGCCCATGAGCCTGTGGTTACTCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTACACGTCCAGAGTGCCTCTCTTGCCATTGACGAGCACCCGCAGTGCCCTGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTACGGTCGTGGCCAAACGGCAGAGGAAGTCGAGCCTGAAGACAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAATCAGATTCTTGCTACTCCCAGCACACACCAACAGCAACTTCCATATCGAAATCCTTATCAGCGACCGAACCACGACCCCCTTCTTTTTGCCAATTATGAAGAAGATTCAGCATCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTAGTTTTCACCATCCAGTTGTTGGAATGATCGGGGATTTCGTGCATGCGAGGGTCTTCGGGAACTCGGATAACTTATACTCTTACCGTAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGACTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAATGATATTGTATGCGTGGAAACACCCCCATTTTCAAGACAAGAAGAAAGAAAGGAAGAAGAAAGAGCAAAAGGAAAAAAAAGAAGATATTTGTTGTCTTTGTATTCTCTGTATAGGCTTTTGGAAGTTAGCTCATGGACAACTAATATGCTTATATACACACTTTTTTTTTACCTTTTTCTGGCTCGTTTAGTACTTTACATAGGTAATCAATATATGTACATGTTCAACTTATAAAATATAGTAGTAAGATTGAACCAATGGCTATGTATTACCTACATAGTGGTTCTTAT

Coding sequence (CDS)

ATGGCCTTTGAGCAGTACTTTGCGCAAGGATGGAAACATGTTTCTGGCACAGAGCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATTTGCTTAGACTTTGCCCATGAGCCTGTGGTTACTCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTACACGTCCAGAGTGCCTCTCTTGCCATTGACGAGCACCCGCAGTGCCCTGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTACGGTCGTGGCCAAACGGCAGAGGAAGTCGAGCCTGAAGACAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAATCAGATTCTTGCTACTCCCAGCACACACCAACAGCAACTTCCATATCGAAATCCTTATCAGCGACCGAACCACGACCCCCTTCTTTTTGCCAATTATGAAGAAGATTCAGCATCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTAGTTTTCACCATCCAGTTGTTGGAATGATCGGGGATTTCGTGCATGCGAGGGTCTTCGGGAACTCGGATAACTTATACTCTTACCGTAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGACTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAA

Protein sequence

MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF

Homology

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 7.2e-50
Identity = 109/237 (45.99%), Postives = 146/237 (61.60%), Query Frame = 0


Query: 11  WKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASL--AIDEH 70
           WK ++    EN  G FDCNICLD  HEPV+TLCGHLYCWPCIYKW++ QS S   +  + 
Sbjct: 24  WKSMNDEVEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQ 83

Query: 71  PQCPVCKADISHTTMVPLYGRGQTAEEVEP-EDKAMHHDINIPPRPSA--CGNQ-ILATP 130
           PQCPVCKA++S  T++PLYGRG   +  +P E KA +  I IP RP +  CG   +L T 
Sbjct: 84  PQCPVCKAEVSEKTLIPLYGRG--GQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTT 143

Query: 131 STHQQQLPYRNPYQRPN--HDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVH 190
            ++  QL  R  YQ+ +    P    +Y    +SP+L+    + +   H    MIG+  +
Sbjct: 144 DSNPSQLLQRRGYQQQSQTRQPAYQGSY---MSSPMLSPGGATANMLQH---SMIGEVAY 203

Query: 191 ARVFGNSD-NLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           AR+FGNS   +Y+Y NSY L  SS  R+RRQ  Q D+SL RI  FLFCC + C+++F
Sbjct: 204 ARIFGNSSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 7.0e-45
Identity = 102/227 (44.93%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 21  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 80
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 81  HTTMVPLYGRGQTAEEV---EPEDKAMHHDINIPPRPSACGNQILATPS-----THQQQL 140
            T++VPLYGRG ++        +  A+  DI   P PSA  N I +  S      HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 141 PYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDN 200
           P  + +Q   + P  F   E        +LA     SF +PV+GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 201 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-39
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 26  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 85
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 86  VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 145
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 146 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 205
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 206 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

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BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-28
Identity = 76/223 (34.08%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 24  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 83
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 84  DISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNP 143
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 144 YQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFG-NSDNLYSY 203
           Y+ P+                              PV+G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 204 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 8.6e-19
Identity = 45/96 (46.88%), Postives = 58/96 (60.42%), Query Frame = 0


Query: 18  ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKAD 77
           ER      F+CNICL+ A E VV++CGHLYCWPC+++WL  +       E  +CPVCKA 
Sbjct: 17  ERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR------PERQECPVCKAG 76

Query: 78  ISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRP 114
           IS   +VPLYGRG      +P+D      +  PPRP
Sbjct: 77  ISREKVVPLYGRGSQ----KPQDPR----LKTPPRP 98

[Close]

BLAST of Cucsat.G2009.T10 vs. NCBI nr
Match: XP_004134707.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743485.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743486.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743487.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743488.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >KGN49224.1 hypothetical protein Csa_003905 [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 5.66e-181
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009.T10 vs. NCBI nr
Match: XP_004134706.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743483.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 1.14e-180
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI
Sbjct: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257

[Close]

BLAST of Cucsat.G2009.T10 vs. NCBI nr
Match: XP_008439861.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK13135.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 488 bits (1256), Expect = 4.12e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009.T10 vs. NCBI nr
Match: XP_038881993.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881995.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881996.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881997.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881998.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 485 bits (1249), Expect = 5.37e-173
Identity = 229/241 (95.02%), Postives = 234/241 (97.10%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAFEQYFAQGWKHVSGT   +RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE KAMHHDINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120

Query: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEEDSA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240

[Close]

BLAST of Cucsat.G2009.T10 vs. NCBI nr
Match: XP_023003183.1 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003184.1 E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 440 bits (1132), Expect = 3.83e-155
Identity = 210/242 (86.78%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MA+EQ FAQGWKHVSGT   +RE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHD-INIPPRPSAC 120
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PE +A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 121 GNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGM 180
           GNQILAT STHQQQL YRNPYQRPNHDPLLF NYEEDS +P LNLARTSFS FHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 181 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 238
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL
Match: A0A0A0KK60 (Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 2.74e-181
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL
Match: A0A5A7UGP7 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007450 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 1.99e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL
Match: A0A1S3AZB8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 1.99e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL
Match: A0A6J1KVR4 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496874 PE=4 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 1.85e-155
Identity = 210/242 (86.78%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MA+EQ FAQGWKHVSGT   +RE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHD-INIPPRPSAC 120
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PE +A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 121 GNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGM 180
           GNQILAT STHQQQL YRNPYQRPNHDPLLF NYEEDS +P LNLARTSFS FHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 181 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 238
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

[Close]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL
Match: A0A6J1CKD0 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111012249 PE=4 SV=1)

HSP 1 Score: 436 bits (1121), Expect = 9.81e-154
Identity = 211/245 (86.12%), Postives = 224/245 (91.43%), Query Frame = 0


Query: 1   MAFEQYFAQGWK-HVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 60
           MAFEQY+A GWK HVS   GT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEQYYAHGWKQHVSSTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSAC 120
           HVQSASLA DEHPQCPVCKADISHTTMVPLYGRGQ+AEEVEPE KA+H DI+IPPRPSAC
Sbjct: 61  HVQSASLATDEHPQCPVCKADISHTTMVPLYGRGQSAEEVEPEAKAIHRDIDIPPRPSAC 120

Query: 121 GNQILATPST--HQQQLPYRNPYQRPNHDPLLFANYEEDS-ASPLLNLARTSFSSFHHPV 180
           GN +LATPS    QQQLPYRNPYQRPNHDPLLF+NYEEDS A+PLLNLARTSFS+FHHPV
Sbjct: 121 GNHMLATPSIDHQQQQLPYRNPYQRPNHDPLLFSNYEEDSSAAPLLNLARTSFSNFHHPV 180

Query: 181 VGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVIL 238
           VGMIGD ++ RVFGNS+NLYSYRNSY +TGSS NRLRRQEMQ  KSLNRISIFL CCVIL
Sbjct: 181 VGMIGDLMYTRVFGNSENLYSYRNSYHVTGSSSNRLRRQEMQASKSLNRISIFLLCCVIL 240

[Close]

BLAST of Cucsat.G2009.T10 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 182.2 bits (461), Expect = 4.9e-46
Identity = 102/227 (44.93%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 21  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 80
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 81  HTTMVPLYGRGQTAEEV---EPEDKAMHHDINIPPRPSACGNQILATPS-----THQQQL 140
            T++VPLYGRG ++        +  A+  DI   P PSA  N I +  S      HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 141 PYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDN 200
           P  + +Q   + P  F   E        +LA     SF +PV+GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 201 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Cucsat.G2009.T10 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-40
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 26  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 85
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 86  VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 145
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 146 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 205
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 206 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Cucsat.G2009.T10 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-40
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 26  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 85
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 86  VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 145
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 146 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 205
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 206 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Cucsat.G2009.T10 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 128.3 bits (321), Expect = 8.5e-30
Identity = 76/223 (34.08%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 24  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 83
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 84  DISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNP 143
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 144 YQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFG-NSDNLYSY 203
           Y+ P+                              PV+G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 204 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 239
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Cucsat.G2009.T10 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 9.1e-24
Identity = 50/101 (49.50%), Postives = 66/101 (65.35%), Query Frame = 0


Query: 15  SGTERENYKGC-FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPV 74
           SG +  N     F+CNICLD A +P+VTLCGHL+CWPC+YKWLH+ S S        CPV
Sbjct: 9   SGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQS------KDCPV 68

Query: 75  CKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPS 115
           CKA I    +VPLYGRG+++   +P  K++   + +P RPS
Sbjct: 69  CKAVIEEDRLVPLYGRGKSS--ADPRSKSI-PGLEVPNRPS 100

The following BLAST results are available for this feature:

BLAST of Cucsat.G2009.T10 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	7.2e-50	45.99	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
Q8GUK7	7.0e-45	44.93	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
O64425	1.5e-39	40.74	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	1.2e-28	34.08	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q99942	8.6e-19	46.88	E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1	[more]

BLAST of Cucsat.G2009.T10 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134707.1	5.66e-181	100.00	E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 ...	[more]
XP_004134706.1	1.14e-180	100.00	E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 ...	[more]
XP_008439861.1	4.12e-174	97.06	PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 ...	[more]
XP_038881993.1	5.37e-173	95.02	E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 u...	[more]
XP_023003183.1	3.83e-155	86.78	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003...	[more]

BLAST of Cucsat.G2009.T10 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK60	2.74e-181	100.00	Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1	[more]
A0A5A7UGP7	1.99e-174	97.06	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3AZB8	1.99e-174	97.06	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 ...	[more]
A0A6J1KVR4	1.85e-155	86.78	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CKD0	9.81e-154	86.12	E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Cucsat.G2009.T10 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	4.9e-46	44.93	RING membrane-anchor 3	[more]
AT4G03510.1	1.1e-40	40.74	RING membrane-anchor 1	[more]
AT4G03510.2	1.1e-40	40.74	RING membrane-anchor 1	[more]
AT4G28270.1	8.5e-30	34.08	RING membrane-anchor 2	[more]
AT1G19310.1	9.1e-24	49.50	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 28..74 e-value: 1.2E-8 score: 44.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 28..75 score: 12.459772
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 28..74 e-value: 3.7E-8 score: 33.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 9..109 e-value: 9.8E-17 score: 62.8
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..238
None	No IPR available	PANTHER	PTHR12313:SF6	E3 UBIQUITIN-PROTEIN LIGASE RMA3	coord: 2..238
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 26..76 e-value: 3.94495E-25 score: 91.5967
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 23..91
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 43..52

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G2009	Cucsat.G2009	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2009.T10.E1	Cucsat.G2009.T10.E1	exon
Cucsat.G2009.T10.E2	Cucsat.G2009.T10.E2	exon
Cucsat.G2009.T10.E3	Cucsat.G2009.T10.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2009.T10.C1	Cucsat.G2009.T10.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G2009.T10	Cucsat.G2009.T10-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding