Cucsat.G2009 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G2009
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	ctg1002: 5450361 .. 5453316 (-)
RNA-Seq Expression	Cucsat.G2009
Synteny	Cucsat.G2009

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACTTCTTCTTATCTCTGTTTCCCGTTCTGTCTTCTTCTCCTTCCTCAACCTTTTTCATTTCTACATTAACATTCCCTCTATTCTCTGCAACCAGTTTTTTCTCTCTATCTCTGTCCCAGGTTAACCACTGAATTTCAACTTTCATCAATACTTTTCTCTTTGTATTCTTGCTCCCTTTCCCTGTGATTGTGTTCGTTGAGGTGGAAGTTTTCACGCTTCTTTCCATTTGTTGATTAATCCATTTTTGTTCTTAATTTCTTACTATTTGTTTGCGATGTTTCATTGTAGCGATCGCGTTCTCAGATTTTCTGGTAGCATTTTTGTTTTCTGGATCTTTCATTCTGGATAAGTTTTCTGTTTATAGCTCTTTTTCTTCCCACTCTGTGTTTCTTTTTAATTTTTTAAAATTGGGAGTGTTGTATTTGGCTGTTGTATGATAATTTTATGTATGTGAGAATACACCAGTGGTTTCATTAGAAATTCTTTTGAATGTTCGGCCTACCGGTTGTACTGTGTATTTATGATTAGAGTTCTCATTCTATGGTTGGAAAGAGCAGGGTTTCTTATGGATCCTTGCGGCTTTGGTTGATGAATTTTCATCATTGACAGCTTTGTTAGGTAATCGACCACTTTTATCTGTTTCTGGTCGATGGAAAATAATCCGAGCAACACACACTGGGAGTAATTTATTTAGCCTTTGGGTCTGACGATTTGATTCAATTTGCTAACACGATCTGTTCTTATATATCTATCATAAAATTAAAAATTAGAACAGCAGAGATTTTTTTCACGTAGGAGAAAAGTTGTTCCCTCTTAATTCTGTATTTTTTTTTTTTTTTTTCGTGTGAAAGCATTTTGAAGCAGTGAGATATCCTCTTGTTTCTTGTCTGCTGTGTGCAGCATTTATCACATTATATGTCTAGAGTTCAGCAAATTCCTAACCATTTGATTTTGGGCTCTTTAAGGAAAACTAGAAGAGGATTTGGTTTTCTGGAGTTACCACTTGAAGTTCCATTTATGACTTTTTCTTGTGGATGGACCCCTAAATATTGAATGGGGAAGAGAAAGGGATAAGAGGTATCACGATTCTGGAGTGGGTATCTAATAAATGAGTACGTTTCTGCCTGCATATTGTGAATATGGGCACACTGAGGAGGACAATGTGCCAGGAATACACTTTTCTTCTTGGCAAACTAGCTAAACAATGATTTAACATTTAGGTCCTCTTCTTCTGTATTTATTCATTGGGCATATGAGTAAATAATGTAGTCATTTTCTTCAATCATTGGGTATGGGTATTGCTTGCTTTACATGTATTATTACTATGGTTAGCTCTAAGTTGACTGCTTTAATTGCTAATTCATTACAATAGCTGTTTTTTGCCATTGTTTCACCATTGCTATTCTATAGAATTTAAGAGCTAAACACATTCAGTTAATCAATTGAAAGCTCTTTGCATTTGAATTTGCCATGTGGCTGATTGATTTTATGGGGCTGTGAGTATTCTAGAATTTTACCCAACTCAATAATTTGGTTGATCTGTTTGCTATTTTATTGATATTACCCATCCTCTCTTACCTAGTAGTAGCTTGCTTCCTTCCATATCTGTGTTCATTTTGTGGTTGGTCTTGAGTAATTAAACTCTTGCAGGATGTGTTAATTAGGCATCTAACTACTCTGAAATAAAGTCGGTCACATGGTTGGTCACTTCCAATTGGGTTTTAGGTATCTTAAATGTCCCACCAACCTTCTATTTGGGTAGTCCGTAATGATCAATTGTTTTTAACTTTCTTCCCACAAACCATAAACTTAGCTTTAAATTCTGTTTTTAATTACTGTTTACCAATATCTTTTCAGATGGGAAAATTTTCCTTATAATTTGTTATCGTGTGCTTTACCAGGAAGCATCTGCAGTTTCTTGACAAATCCATGGCCTTTGAGCAGTACTTTGCGCAAGGATGGAAACATGTTTCTGGCACAGAGCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATTTGCTTAGACTTTGCCCATGAGCCTGTGGTTACTCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTACACGTCCAGAGTGCCTCTCTTGCCATTGACGAGCACCCGCAGTGCCCTGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTACGGTCGTGGCCAAACGGCAGAGGAAGTCGAGCCTGAAGACAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAATCAGATTCTTGCTACTCCCAGCACACACCAACAGCAACTTCCATATCGAAATCCTTATCAGCGACCGAACCACGACCCCCTTCTTTTTGCCAATTATGAAGAAGATTCAGCATCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTAGTTTTCACCATCCAGTTGTTGGAATGATCGGGGATTTCGTGCATGCGAGGGTCTTCGGGAACTCGGATAACTTATACTCTTACCGTAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGACTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAATGATATTGTATGCGTGGAAACACCCCCATTTTCAAGACAAGAAGAAAGAAAGGAAGAAGAAAGAGCAAAAGGAAAAAAAAGAAGATATTTGTTGTCTTTGTATTCTCTGTATAGGCTTTTGGAAGTTAGCTCATGGACAACTAATATGCTTATATACACACTTTTTTTTTACCTTTTTCTGGCTCGTTTAGTACTTTACATAGGTAATCAATATATGTACATGTTCAACTTATAAAATATAGTAGTAAGATTGAACCAATGGCTATGTATTACCTACATAGTGGTTCTTATTATTCTTATTACTCAAATTAGAAGCCGTTTTCTTTATTTATGTGTTTATCTTATGTTATCGACTAGTTGTTATGTGC

Coding sequence (CDS)

ATGGTTGGTCACTTCCAATTGGGTTTTAGGAAGCATCTGCAGTTTCTTGACAAATCCATGGCCTTTGAGCAGTACTTTGCGCAAGGATGGAAACATGTTTCTGGCACAGAGCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATTTGCTTAGACTTTGCCCATGAGCCTGTGGTTACTCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTACACGTCCAGAGTGCCTCTCTTGCCATTGACGAGCACCCGCAGTGCCCTGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTTTACGGTCGTGGCCAAACGGCAGAGGAAGTCGAGCCTGAAGACAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAATCAGATTCTTGCTACTCCCAGCACACACCAACAGCAACTTCCATATCGAAATCCTTATCAGCGACCGAACCACGACCCCCTTCTTTTTGCCAATTATGAAGAAGATTCAGCATCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTAGTTTTCACCATCCAGTTGTTGGAATGATCGGGGATTTCGTGCATGCGAGGGTCTTCGGGAACTCGGATAACTTATACTCTTACCGTAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGACTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAA

Protein sequence

MVGHFQLGFRKHLQFLDKSMAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF

Homology

[Close]

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.0e-49
Identity = 109/237 (45.99%), Postives = 146/237 (61.60%), Query Frame = 0


Query: 30  WKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASL--AIDEH 89
           WK ++    EN  G FDCNICLD  HEPV+TLCGHLYCWPCIYKW++ QS S   +  + 
Sbjct: 24  WKSMNDEVEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQ 83

Query: 90  PQCPVCKADISHTTMVPLYGRGQTAEEVEP-EDKAMHHDINIPPRPSA--CGNQ-ILATP 149
           PQCPVCKA++S  T++PLYGRG   +  +P E KA +  I IP RP +  CG   +L T 
Sbjct: 84  PQCPVCKAEVSEKTLIPLYGRG--GQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTT 143

Query: 150 STHQQQLPYRNPYQRPN--HDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVH 209
            ++  QL  R  YQ+ +    P    +Y    +SP+L+    + +   H    MIG+  +
Sbjct: 144 DSNPSQLLQRRGYQQQSQTRQPAYQGSY---MSSPMLSPGGATANMLQH---SMIGEVAY 203

Query: 210 ARVFGNSD-NLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           AR+FGNS   +Y+Y NSY L  SS  R+RRQ  Q D+SL RI  FLFCC + C+++F
Sbjct: 204 ARIFGNSSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

[Close]

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 7.5e-45
Identity = 102/227 (44.93%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 40  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 99
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 100 HTTMVPLYGRGQTAEEV---EPEDKAMHHDINIPPRPSACGNQILATPS-----THQQQL 159
            T++VPLYGRG ++        +  A+  DI   P PSA  N I +  S      HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 160 PYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDN 219
           P  + +Q   + P  F   E        +LA     SF +PV+GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 220 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-39
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 45  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 104
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 164
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 165 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 224
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 225 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-28
Identity = 76/223 (34.08%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 43  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 102
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 103 DISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNP 162
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 163 YQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFG-NSDNLYSY 222
           Y+ P+                              PV+G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 223 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.3e-19
Identity = 45/96 (46.88%), Postives = 58/96 (60.42%), Query Frame = 0


Query: 37  ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKAD 96
           ER      F+CNICL+ A E VV++CGHLYCWPC+++WL  +       E  +CPVCKA 
Sbjct: 17  ERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETR------PERQECPVCKAG 76

Query: 97  ISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRP 133
           IS   +VPLYGRG      +P+D      +  PPRP
Sbjct: 77  ISREKVVPLYGRGSQ----KPQDPR----LKTPPRP 98

[Close]

BLAST of Cucsat.G2009 vs. NCBI nr
Match: XP_004134706.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743483.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus])

HSP 1 Score: 544 bits (1401), Expect = 1.39e-195
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0


Query: 1   MVGHFQLGFRKHLQFLDKSMAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVT 60
           MVGHFQLGFRKHLQFLDKSMAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVT
Sbjct: 1   MVGHFQLGFRKHLQFLDKSMAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDK 120
           LCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDK
Sbjct: 61  LCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDK 120

Query: 121 AMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNL 180
           AMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNL
Sbjct: 121 AMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNL 180

Query: 181 ARTSFSSFHHPVVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLN 240
           ARTSFSSFHHPVVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLN
Sbjct: 181 ARTSFSSFHHPVVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLN 240

Query: 241 RISIFLFCCVILCVLVF 257
           RISIFLFCCVILCVLVF
Sbjct: 241 RISIFLFCCVILCVLVF 257

[Close]

BLAST of Cucsat.G2009 vs. NCBI nr
Match: XP_004134707.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743485.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743486.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743487.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743488.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >KGN49224.1 hypothetical protein Csa_003905 [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 1.23e-180
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79
           MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120

Query: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009 vs. NCBI nr
Match: XP_008439861.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK13135.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 488 bits (1256), Expect = 8.91e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009 vs. NCBI nr
Match: XP_038881993.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881995.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881996.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881997.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881998.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 485 bits (1249), Expect = 1.16e-172
Identity = 229/241 (95.02%), Postives = 234/241 (97.10%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 79
           MAFEQYFAQGWKHVSGT   +RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 80  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 139
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE KAMHHDINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120

Query: 140 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 199
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEEDSA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180

Query: 200 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 257
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240

[Close]

BLAST of Cucsat.G2009 vs. NCBI nr
Match: XP_023003183.1 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003184.1 E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 440 bits (1132), Expect = 8.21e-155
Identity = 210/242 (86.78%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 79
           MA+EQ FAQGWKHVSGT   +RE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 80  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHD-INIPPRPSAC 139
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PE +A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 140 GNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGM 199
           GNQILAT STHQQQL YRNPYQRPNHDPLLF NYEEDS +P LNLARTSFS FHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 200 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 257
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

[Close]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL
Match: A0A0A0KK60 (Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 5.94e-181
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79
           MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 120

Query: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL
Match: A0A5A7UGP7 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007450 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 4.32e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL
Match: A0A1S3AZB8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 4.32e-174
Identity = 231/238 (97.06%), Postives = 233/238 (97.90%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 79
           MAF+QYFAQGWKHVSGT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS
Sbjct: 1   MAFDQYFAQGWKHVSGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQS 60

Query: 80  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQI 139
           ASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE E KAMH DINIPPRPSACGNQI
Sbjct: 61  ASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACGNQI 120

Query: 140 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDF 199
           LATPSTHQQQLPYRNPYQRPNHDPLLFANYEE SASPLLNLARTSFSSFHHPVVGMIGDF
Sbjct: 121 LATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMIGDF 180

Query: 200 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 257
           VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF
Sbjct: 181 VHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 238

[Close]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL
Match: A0A6J1KVR4 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496874 PE=4 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 3.98e-155
Identity = 210/242 (86.78%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 20  MAFEQYFAQGWKHVSGT---ERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 79
           MA+EQ FAQGWKHVSGT   +RE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 80  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHD-INIPPRPSAC 139
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PE +A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 140 GNQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGM 199
           GNQILAT STHQQQL YRNPYQRPNHDPLLF NYEEDS +P LNLARTSFS FHHPVVGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 200 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 257
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

[Close]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL
Match: A0A6J1CKD0 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111012249 PE=4 SV=1)

HSP 1 Score: 436 bits (1121), Expect = 2.10e-153
Identity = 211/245 (86.12%), Postives = 224/245 (91.43%), Query Frame = 0


Query: 20  MAFEQYFAQGWK-HVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 79
           MAFEQY+A GWK HVS   GT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEQYYAHGWKQHVSSTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 60

Query: 80  HVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSAC 139
           HVQSASLA DEHPQCPVCKADISHTTMVPLYGRGQ+AEEVEPE KA+H DI+IPPRPSAC
Sbjct: 61  HVQSASLATDEHPQCPVCKADISHTTMVPLYGRGQSAEEVEPEAKAIHRDIDIPPRPSAC 120

Query: 140 GNQILATPST--HQQQLPYRNPYQRPNHDPLLFANYEEDS-ASPLLNLARTSFSSFHHPV 199
           GN +LATPS    QQQLPYRNPYQRPNHDPLLF+NYEEDS A+PLLNLARTSFS+FHHPV
Sbjct: 121 GNHMLATPSIDHQQQQLPYRNPYQRPNHDPLLFSNYEEDSSAAPLLNLARTSFSNFHHPV 180

Query: 200 VGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVIL 257
           VGMIGD ++ RVFGNS+NLYSYRNSY +TGSS NRLRRQEMQ  KSLNRISIFL CCVIL
Sbjct: 181 VGMIGDLMYTRVFGNSENLYSYRNSYHVTGSSSNRLRRQEMQASKSLNRISIFLLCCVIL 240

[Close]

BLAST of Cucsat.G2009 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 182.2 bits (461), Expect = 5.3e-46
Identity = 102/227 (44.93%), Postives = 135/227 (59.47%), Query Frame = 0


Query: 40  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 99
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 100 HTTMVPLYGRGQTAEEV---EPEDKAMHHDINIPPRPSACGNQILATPS-----THQQQL 159
            T++VPLYGRG ++        +  A+  DI   P PSA  N I +  S      HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 160 PYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNSDN 219
           P  + +Q   + P  F   E        +LA     SF +PV+GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 220 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Cucsat.G2009 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 45  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 104
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 164
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 165 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 224
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 225 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Cucsat.G2009 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 88/216 (40.74%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 45  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 104
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNPYQRPNHD 164
           VPLYGRG+   + E ++      +    R     +   +T     Q++ + +P +   + 
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE--GYY 165

Query: 165 PLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 224
           P+          S +++    S+S+   PV+ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 PV----------SGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 225 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Cucsat.G2009 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 128.3 bits (321), Expect = 9.2e-30
Identity = 76/223 (34.08%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 43  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 102
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 103 DISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACGNQILATPSTHQQQLPYRNP 162
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 163 YQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMIGDFVHARVFG-NSDNLYSY 222
           Y+ P+                              PV+G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 223 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 258
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Cucsat.G2009 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 50/101 (49.50%), Postives = 66/101 (65.35%), Query Frame = 0


Query: 34  SGTERENYKGC-FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPV 93
           SG +  N     F+CNICLD A +P+VTLCGHL+CWPC+YKWLH+ S S        CPV
Sbjct: 9   SGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQS------KDCPV 68

Query: 94  CKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPS 134
           CKA I    +VPLYGRG+++   +P  K++   + +P RPS
Sbjct: 69  CKAVIEEDRLVPLYGRGKSS--ADPRSKSI-PGLEVPNRPS 100

The following BLAST results are available for this feature:

BLAST of Cucsat.G2009 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	1.0e-49	45.99	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
Q8GUK7	7.5e-45	44.93	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
O64425	1.6e-39	40.74	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	1.3e-28	34.08	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q99942	9.3e-19	46.88	E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1	[more]

BLAST of Cucsat.G2009 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134706.1	1.39e-195	100.00	E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 ...	[more]
XP_004134707.1	1.23e-180	100.00	E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 ...	[more]
XP_008439861.1	8.91e-174	97.06	PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 ...	[more]
XP_038881993.1	1.16e-172	95.02	E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 u...	[more]
XP_023003183.1	8.21e-155	86.78	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003...	[more]

BLAST of Cucsat.G2009 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK60	5.94e-181	100.00	Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1	[more]
A0A5A7UGP7	4.32e-174	97.06	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3AZB8	4.32e-174	97.06	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 ...	[more]
A0A6J1KVR4	3.98e-155	86.78	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CKD0	2.10e-153	86.12	E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Cucsat.G2009 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	5.3e-46	44.93	RING membrane-anchor 3	[more]
AT4G03510.1	1.2e-40	40.74	RING membrane-anchor 1	[more]
AT4G03510.2	1.2e-40	40.74	RING membrane-anchor 1	[more]
AT4G28270.1	9.2e-30	34.08	RING membrane-anchor 2	[more]
AT1G19310.1	1.3e-23	49.50	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 28..74 e-value: 1.2E-8 score: 44.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 28..75 score: 12.459772
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 28..74 e-value: 3.7E-8 score: 33.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 9..109 e-value: 9.8E-17 score: 62.8
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..238
None	No IPR available	PANTHER	PTHR12313:SF6	E3 UBIQUITIN-PROTEIN LIGASE RMA3	coord: 2..238
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 26..76 e-value: 3.94495E-25 score: 91.5967
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 23..91
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 43..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G2009.T11	Cucsat.G2009.T11	mRNA
Cucsat.G2009.T4	Cucsat.G2009.T4	mRNA
Cucsat.G2009.T6	Cucsat.G2009.T6	mRNA
Cucsat.G2009.T1	Cucsat.G2009.T1	mRNA
Cucsat.G2009.T12	Cucsat.G2009.T12	mRNA
Cucsat.G2009.T13	Cucsat.G2009.T13	mRNA
Cucsat.G2009.T5	Cucsat.G2009.T5	mRNA
Cucsat.G2009.T2	Cucsat.G2009.T2	mRNA
Cucsat.G2009.T8	Cucsat.G2009.T8	mRNA
Cucsat.G2009.T15	Cucsat.G2009.T15	mRNA
Cucsat.G2009.T7	Cucsat.G2009.T7	mRNA
Cucsat.G2009.T16	Cucsat.G2009.T16	mRNA
Cucsat.G2009.T14	Cucsat.G2009.T14	mRNA
Cucsat.G2009.T10	Cucsat.G2009.T10	mRNA
Cucsat.G2009.T3	Cucsat.G2009.T3	mRNA
Cucsat.G2009.T9	Cucsat.G2009.T9	mRNA
Cucsat.G2009.T18	Cucsat.G2009.T18	mRNA
Cucsat.G2009.T17	Cucsat.G2009.T17	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity