Cucsat.G19868.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19868.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionvesicle-associated protein 2-2 isoform X1
Locationctg4: 2336253 .. 2341849 (+)
RNA-Seq ExpressionCucsat.G19868.T1
SyntenyCucsat.G19868.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAAGAAAAAGCCTCGAAATTGGAGCCAATTTTGTTAAATTATTTGAAAAGAAAAGAGAAAGGGAAGAAAGTTCAAACGGGAATTGGATTCTGCCGCACCGGCTCAAAGCTAAGATCACACCACTGCTGGTACAACTAACACTACCTCTCTCTCTCTCTCTACTTTGCTTTCACTCAGTTTAATCCTCCATTCCACCAACTAGATCATTCATTCTCACCTTCCTCTCTCTAGAATTTTCCAGGGTTTCTCTCTCTCTCTCCCTTCATATGGAATTGTAAATTCCACCAAAATCTTTGGACTCTCTTCCAATTTCTGCATTCCATTCTCTTTTATTTATTCCATTTCAGTTTATCTTTCTCTCTCTATCTCCGTTCCCGCGGAACTGGTGAGTCTTTTGGATTCTTTTTCCGCTTCTTGTTTTATATTCTGCGTGGAAGAATCTTATTGTTTGGTTATTTTTGTTGTTTGAAATTCATGGTATCTTGTTAAGAACGCAGTCTGTGTGTTTTTTCCTGAATTTCTGCTGGTTTCTTGAATATTGGAGTTTCTTTTTTGCCTCTCGATTTGTGTTTGATTTTGTTGAGTTTGGAAATGGGTTGGAGTTTGTAATCGGATTAGTGTTTGGAGTGACTCTGTTCGTTGACTAATGATTTATGGTTCGAGTAGTGTTTGGTTTGTAACTTTGAACGATCTGTTTTGGAATAAGCCCAGCGGTGCGTCTTTGATTTTGATCTTAGTGGGTTTGGTTTGACTTTCTCAACCCAGTTTTTTTTTTTCCTTGGAAAGTTGGGGAAATGGGATATTATTATTATTTTATTTTAGCCAGGAACAGGGTGGATTGGAAACACGTGGTTTTTAGCTTCTATGATCTGTAATTATATGATGAAATACTTGGGATTATTCTATGTTTCTTTTTTTCTTTCTTTTTATTTTTTATTTTTTGAATTTTCTTCTAAATCAAATTTAATTTGTTTTTCCCGATTATAAGAAAATAACGTTTCTGTGTGCAGAGTAATTGTTTCCCAGGCTTTGAATGCAGAAGTATTAATTTTGAATTGTGTATTGTGAGGAAGAGACATGAGTACGAAACTCTTGCATATCCAGCCTAAGGAATTACAATTCTTATGTAAGTGCATTACTAGTAATACTAGTCATTGCTTGCTTTTTGAAAAAGAATTGATGTAAAGTACCTTGCTACTTTCAGTTGAATTGAGGAAACAAAGTACGTGCACAGTTCAACTATCCAATAATACCCATCATCATGTTGCCTTTAAAGTATGAACAACCTTCTTTTCTTTTTGTTCATCTCTTGTTTCTTTGATAATTCATATGATTTTTCATGTCATGTGTGCAATGTTATTATTATGTTTGGCAGGTTAAAACTACGTCTCCTAAGAAATATTGTGTTCGACCAAATGTTGGGATAATTTTGCCAAAATCAACATGTGAATTTTCAGGTACATTCTTTATAATTTTCTTCTAATCTAACATATTTGGCCACCTGGGACATTTGATTCCCGGTCCCCCAGAAACAAAAAAGAGAGTGTTTCTAGATATTTTAACGAAGTTACTACCATGATGATTGACGTTTCAATGAAGTTGATTCTTCCATCTAGTACTTGACATGTACAGTATCTGATCGGCTCAATGTCTTTCTTTAGTTGAATCAAAATTATGAGGACTATCTGGAAAGTTTTAAAAGGGAAATTTGAGAACATATCACTTTAGTGTTTTGAGGAATAAAAACTAAGAATGTCTAATATCATGTCATGATTCAATCCTGCATTTAACAACTATTTCTCCCTTACAGTTTCTTTTAGGAAGACTGTGCAACTGTCTTACTGTAATACTTGCGTCCTTTTTTTTTCTTTGCTTTTGTTCTTGTTTTTCCTAAAAAGAAAACCTAAACCTGGAAGTTGGCTGTGTATTGTTGCTTTTCCTCCTCTTTTTAGAAGCTAATTTCTTCTATGATTCTGACGTAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTATGTAAAGACAAGTTTTTAATTCAAAGCACAATCGTTCCTTCAGGGATAACAGAAGAGGACATCACTGCAAGTATGGTAAGTTTTTTGGATACATTCAGCTTTCATGCCTTGGGTTTTGAACTGTTTACATTTCTTTCCAGTTTTCAAAAGATGGAGGCAAATATATTGAAGAGGATAAGCTCAAAGTGGCGCTCATTAGTCCGCTGAATTCCCCAATACTCTCGCCTTCTGATGGAGCATTGGACGCTAGAATGGATGACGTTATGAAATTGAATGAAGATAGTGGACCATTAAGTGATGGGATGGACACCAATGAAACCCCAAAGCTGAAAGATCAAAATGGTGCATCAGATGAGGTACTTCCCTCCGTGGTTCCAATCCTAAGATATCAAAATGGACTATCAAATGAGGGCATGACCCATGAGGCTTCAAGATTCAAAGATGATTTTGTTGCATTGAAGGGGGAGCGGACCATTGAAGCTTTAAAGCCATCTGATCAAAATGGAGCATTCAGGGAGGAGGTTCTGCCTCATGAGTTTTCAAAACTGAAGGATCCAAATGAAACATCAAAGGAAGAGCTACCTCGTGAAGCATTAAAACCGAGAGATCAAAATCGAGCATTAAAGGAAGAGGAGTTGAGTGTTGAATCACCAACAAAAAAAGTTCAAAATGGTGCACTGAAGTGGGGAGCCTACGGAAGTTCACATCTGGAGAATCCAGTACCAGACACAAAAGTAAGAAAAAACTTTCCTTATCAAATTTGATGATATACTGACTAATTTTTGTTGTTCTAAGAAATTTTTGGAGCCAGAGGTCTCTTTATAGTAACCTCTTCCACATTTCCTAGGGAGGGTGCCTTATCTTTTCCTTGTTTTTCCCAGTAGAAAGTGTTTCTTTTCTAGAGAGGAAAAAAAAAAATTGTAGTAGGAAACTGTAGACCGTGTCATGATTTAGGCATTGTTCAATTTGAGATTTTCAGTTTCCTGTGCATTGGCTTCTAGAACATTGAAGTTTTTCTCTTGTGCATCAGTTTCCTGAGGATGCGGATGAATCCCTAGAGCTAAACAATAGCATGTCAAGATTACAAAAGACTGCTGAGATAAAGCAAGGCACAGAATTGGAACCTTTGAAACTGAAAAAAGCGGAACAGCTACAGTTAGTTAAGGACATTGATGAGATGAAATCGAAGCTACATGAAATTGAATTAAAGCTTGGAGAGGTGATTTTTTATTGCCTTTTGTGCTTTTCTCGTTCTTGTGCTGCCTTTAAGCTATTATTGAAAATCTGATCATTGAGATTGTTGATCCTCACCTAATTCAATTAAGAAATAGGACTGTGATTGATTTTTAATTTTGTTGATGCGCATGCGTATTCAATTTATCTATGAACTTACCATAACAGTTGATTTGTAGAAAGAACCAGCGACCTATGTCAAACAACTTTTAGATAAAAAACTGAAATTTATTCAGAAAAAGAGAGCTTGTTAAATCATCATTTGACCCTAAAGCTTAAACTAGTGGGTTATGGTAAATTTTATTATACAAACACTTAAACACTCTTACCCACTTTTGGGCTTGAAATTTTGTAGATGACCGAATGAATAGAAATTAATATTATGTGGAGAGAAAATGACATTACACCTATAGAAGGGAGGGGAGGAGAGGGGGGGGAGGGGAGGGGGAGGGGAGGGCGACGAGTTCTCCCTAAAAATCTTTGTAGAATATTAAGAAAATCACTCCCAAAAGAATTAGTTCTGAAACCATTAGAAAGAAATCTTCATAAAGAAGCCAAAAAATCAAGCCTTGTCCCGAAAATGATCAAGAATCTTATATGCCGTTTGAAAGTTTGCACTCTGTTTGTTAAAACAATTTCCTGATGAGATTCATTTCACCCTGTTGCAAATTGCACTCCCTGAAATTTGTTAAACTTGGTTCATTTGCTAATTCATTTGGATGTTCAGTAATGCAACTTTTGTGGTTGATTTTGATTTTTCACTGCATCTAGATAAGCTTGGTTTTGTGATACAGGTTGATTCTTAAAATTTGTTTAAACTTGGTCTCAGTTGCCGGTTATCATCTGTTTTTTTTCCTTTTCCCTTCGAGGTTTGCAATGTTTTTCCTTAATTAGATGAAGAATTAGGCGAGGGCTTCCTTTAATCATAATCATCTCAGTACCCAAGAAATGAAATGTTGATGGAAAGGAAAGAAACTACTTCACTAATATTCATTTTTCATTTGCCGTATTATTGTACTTTTTATTGACCAACACAATTGTTAATATGTTTTTTTGCCACATCATTTAATAGGCTCAAGGTACCATTTCAATGCTGTCTGAAGCGAGGAGATTAAGTGCTCAAGAAGTGAAAATCTTGAAGGAGAAATTGGTAACTTCCTGGACCTTATTTTTCTGTTAGTGGATGTTCTTCTTCCATGTTAGAGTCTTATGATGTCCATTTGATATTCAAGCCTTGCCACCCCAAGGGACCCTAATATTAGAGAGATTAGGTACCTTTTTCCCCAGCCGTAAGGATTGGAGATGTATTCAAGGTACTAGGTTGAAACTTCTCGCTTGAAAATTTGAAATCGTTACTGTCTCACTTTGGAGTGGTTGAAGTCCTCTCAGGGTGTACGGGCCTCTCAGTTTTAGTTGGTGGAATGGGATATTATGGCCCACGGGGCTCTAACTATAACAGATTAACTCAGCGCCCCAGCACCACCTCCTTACAATTTAGTTTGAGGTGTACTAATACTAATGCAAAGTAGAAGGAATAGAGTGCCTAAATGTCTGTTGTCCCATCAAGAAAAAATAGTGCAGGGGTAGCTTTAGTGGAGCCATCTCAAAAGTAATATTGTTATTTAGTTCACTTGTTTTGATCTTTTTGGACACTCTGCTCTCAGAAATTCATTCTTAACTGTACTTGTGCTTCCGGATTTTGTATACTATTGGATTTCAGCTGGTTCCTCAATAGCTGCATTCATGGTTTCTCTCACTTCCATTTCTTCTATCATTACTTCTCGTGACTCAGTGTTTTATCTTACTATTTTCTCAGCTGGAACTGAGTAGGAGGGGCAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGTTAATTTGCATTGTGTTCGGATTCGTTTTGCACCATTGAAGTGTAAGGTGGGGAGGAGATGAGATGAGGATTGCAGAAAATATGTAAAGAAAAATATTTTACTTATGTAGATAGATCAGTAGCTAGTGCGGTGTGTTGTCAATAAGATGCACATTTTGATTTTGGGCACTGGAAGTTTGCTGCTCTTAGTATGCAATTCAGATTTTGGGTATAAATCTAACACGATGCTCATCTGCTTTTACCAATGTTTGAAGATGAGTTGTGATGGTTGACCAAATGTTGGGTTTCATATTGGTCTTCTTTTCTCTCTTTTCCCTTTTTCTTTTTGTGATTTGTTTCTGAGTTAGTCTATGTGCTGATTCTAACCGAATTTGAACTTTTTTTTTTTTGTATTGGAAAGTCATACTTGTTCAGTTGTGTTCTAATATGAGCATTTTTGGTCTTCTATG

Coding sequence (CDS)

ATGAGTACGAAACTCTTGCATATCCAGCCTAAGGAATTACAATTCTTATTTGAATTGAGGAAACAAAGTACGTGCACAGTTCAACTATCCAATAATACCCATCATCATGTTGCCTTTAAAGTTAAAACTACGTCTCCTAAGAAATATTGTGTTCGACCAAATGTTGGGATAATTTTGCCAAAATCAACATGTGAATTTTCAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTATGTAAAGACAAGTTTTTAATTCAAAGCACAATCGTTCCTTCAGGGATAACAGAAGAGGACATCACTGCAAGTATGTTTTCAAAAGATGGAGGCAAATATATTGAAGAGGATAAGCTCAAAGTGGCGCTCATTAGTCCGCTGAATTCCCCAATACTCTCGCCTTCTGATGGAGCATTGGACGCTAGAATGGATGACGTTATGAAATTGAATGAAGATAGTGGACCATTAAGTGATGGGATGGACACCAATGAAACCCCAAAGCTGAAAGATCAAAATGGTGCATCAGATGAGGTACTTCCCTCCGTGGTTCCAATCCTAAGATATCAAAATGGACTATCAAATGAGGGCATGACCCATGAGGCTTCAAGATTCAAAGATGATTTTGTTGCATTGAAGGGGGAGCGGACCATTGAAGCTTTAAAGCCATCTGATCAAAATGGAGCATTCAGGGAGGAGGTTCTGCCTCATGAGTTTTCAAAACTGAAGGATCCAAATGAAACATCAAAGGAAGAGCTACCTCGTGAAGCATTAAAACCGAGAGATCAAAATCGAGCATTAAAGGAAGAGGAGTTGAGTGTTGAATCACCAACAAAAAAAGTTCAAAATGGTGCACTGAAGTGGGGAGCCTACGGAAGTTCACATCTGGAGAATCCAGTACCAGACACAAAATTTCCTGAGGATGCGGATGAATCCCTAGAGCTAAACAATAGCATGTCAAGATTACAAAAGACTGCTGAGATAAAGCAAGGCACAGAATTGGAACCTTTGAAACTGAAAAAAGCGGAACAGCTACAGTTAGTTAAGGACATTGATGAGATGAAATCGAAGCTACATGAAATTGAATTAAAGCTTGGAGAGGCTCAAGGTACCATTTCAATGCTGTCTGAAGCGAGGAGATTAAGTGCTCAAGAAGTGAAAATCTTGAAGGAGAAATTGCTGGAACTGAGTAGGAGGGGCAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGTTAATTTGCATTGTGTTCGGATTCGTTTTGCACCATTGA

Protein sequence

MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILPKSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKLKVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH
Homology
BLAST of Cucsat.G19868.T1 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.3e-57
Identity = 163/431 (37.82%), Postives = 233/431 (54.06%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           M+  LL IQP+ LQF  +L+KQ++C VQL+N THH+VAFKVKTTSPKKYCVRPNVG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEF+VIMQA +  PPDM+CKDKFLIQST V +  T+EDITASMFSK  GK+IEE+KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           +V L+ P +SP LSP            +   +      D +       LKD+  +  E L
Sbjct: 121 RVTLVPPSDSPELSP------------INTPKQGAVFEDSI-------LKDRLYSQSETL 180

Query: 181 PSVVPILRYQNGLSNEG--MTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHE 240
                  +Y+  +  E   + H+  +  D+   LK  +        D+  A   +     
Sbjct: 181 APP----QYEGEIVKEPRMVGHDELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDS 240

Query: 241 FSKLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLEN 300
           +   +   ET  +  P  + K  D  RA+K    ++++P KK  +     G         
Sbjct: 241 YDSSRMAKETGFD--PIRSHKDADDGRAIK-ATTNLDAPMKKAMDLPRDQGFTNG----- 300

Query: 301 PVPDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKL 360
                    D++  +     + +LQKT         +   ++  ++L+LVKDI+EMK K+
Sbjct: 301 ------IAVDSEPKISKERDVVQLQKT---------DGQNVRGLDELKLVKDIEEMKLKV 360

Query: 361 HEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQV--GFPPLFICMV 420
             +E KL +A  TIS L E R +S+Q  + L+ +L EL R  +   +V  GFP L++C+V
Sbjct: 361 DALESKLKQADSTISKLMEERSISSQHRQSLQHELAEL-RTKKIVKEVHNGFPLLYVCVV 384

Query: 421 ALICIVFGFVL 428
           A I  V G  L
Sbjct: 421 AFIAYVIGHFL 384

BLAST of Cucsat.G19868.T1 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.3e-37
Identity = 71/130 (54.62%), Postives = 99/130 (76.15%), Query Frame = 0

Query: 2   STKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILPK 61
           +++LL ++P +LQF FEL+KQ +C++ L+N T ++VAFKVKTT+PKKYCVRPN G++LP+
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKLK 121
           STCE  V MQAQ+ AP DM CKDKFL+Q  I   G+T +++T  MFSK+ G  +EE KL+
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VALISPLNSP 132
           V  ++P   P
Sbjct: 139 VTYVAPPRPP 148

BLAST of Cucsat.G19868.T1 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.0e-35
Identity = 70/131 (53.44%), Postives = 94/131 (71.76%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MS +LL I P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           +S+ E  V MQAQ+ AP D+ CKDKFL+Q  +   G T +D+T  MFSK+ G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 KVALISPLNSP 132
           +V  ++P   P
Sbjct: 121 RVVYVAPPRPP 131

BLAST of Cucsat.G19868.T1 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.8e-34
Identity = 77/155 (49.68%), Postives = 102/155 (65.81%), Query Frame = 0

Query: 4   KLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILPKST 63
           +L+ IQP EL+FLFEL KQS C ++++N T ++VAFKVKTTSPKKY VRPN G+I P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  CEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKLKVA 123
           C   V +QAQR  PPDM CKDKFL+QSTIVP     +++    F+KD GK + E KLKV+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 LISPLNSPILSPS-----DGALDARMDDVMKLNED 154
            I+P  +   S S     DG     +  + +L E+
Sbjct: 128 YITPSTTQRSSESGATNGDGQSSETISTIQRLKEE 162

BLAST of Cucsat.G19868.T1 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.7e-33
Identity = 80/169 (47.34%), Postives = 113/169 (66.86%), Query Frame = 0

Query: 5   LLHIQPKELQFLFELRKQSTCTVQLSNNTHHH-VAFKVKTTSPKKYCVRPNVGIILPKST 64
           L++I P EL+F FEL+KQS+C++QL+N T    VAFKVKTT+P+KYCVRPN G++LP  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  CEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKLKVA 124
           C  +V MQAQ+ AP DM CKDKFL+Q+ +V  G T +++ A MF+K+ G+ IE+ KL+V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LI-----SPL-------NSPILSPSDGALDAR--MDDVMKLNEDSGPLS 159
            I     SP+       NSP+ S +D A  +    DDV +  E++   S
Sbjct: 126 YIPANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSEKS 174

BLAST of Cucsat.G19868.T1 vs. NCBI nr
Match: XP_011648789.1 (vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_011648790.1 vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_031737157.1 vesicle-associated protein 2-2 isoform X1 [Cucumis sativus])

HSP 1 Score: 829 bits (2142), Expect = 1.02e-302
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL
Sbjct: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS
Sbjct: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           +LKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV
Sbjct: 241 RLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420

Query: 421 IVFGFVLHH 429
           IVFGFVLHH
Sbjct: 421 IVFGFVLHH 429

BLAST of Cucsat.G19868.T1 vs. NCBI nr
Match: XP_008455504.1 (PREDICTED: vesicle-associated protein 2-2 isoform X1 [Cucumis melo])

HSP 1 Score: 776 bits (2005), Expect = 7.72e-282
Identity = 403/429 (93.94%), Postives = 411/429 (95.80%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPI SP DGALD RMDDVMKLNE SGPL DGMDTNE PKLKDQNGASDEVL
Sbjct: 121 KVALISPLNSPIHSPFDGALDGRMDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDEVL 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PS+VPI+RYQNG SNE MT EASRFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S
Sbjct: 181 PSLVPIVRYQNGASNEDMTLEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           + KDPNETSKEELPRE LKPRDQNRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PV
Sbjct: 241 RPKDPNETSKEELPREVLKPRDQNRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPED D+SLELNNSM RLQKTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDVDKSLELNNSMPRLQKTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGR SNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALIC 420

Query: 421 IVFGFVLHH 429
           IV GFVLHH
Sbjct: 421 IVLGFVLHH 429

BLAST of Cucsat.G19868.T1 vs. NCBI nr
Match: XP_004139205.1 (vesicle-associated protein 2-2 isoform X2 [Cucumis sativus] >KAE8651570.1 hypothetical protein Csa_019344 [Cucumis sativus])

HSP 1 Score: 742 bits (1916), Expect = 7.51e-269
Identity = 393/429 (91.61%), Postives = 394/429 (91.84%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDE  
Sbjct: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDE-- 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
                                            GERTIEALKPSDQNGAFREEVLPHEFS
Sbjct: 181 ---------------------------------GERTIEALKPSDQNGAFREEVLPHEFS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           +LKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV
Sbjct: 241 RLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 394

Query: 421 IVFGFVLHH 429
           IVFGFVLHH
Sbjct: 421 IVFGFVLHH 394

BLAST of Cucsat.G19868.T1 vs. NCBI nr
Match: XP_038890494.1 (vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-associated protein 2-2-like [Benincasa hispida])

HSP 1 Score: 719 bits (1855), Expect = 7.64e-259
Identity = 382/439 (87.02%), Postives = 399/439 (90.89%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQF+FELRKQSTCTVQL+NNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQR APPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRVAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPIL PSDGA DARMD+VMKLNE SGPL DGMDTNETPKLKDQNGASDEVL
Sbjct: 121 KVALISPLNSPILLPSDGAWDARMDEVMKLNEHSGPLRDGMDTNETPKLKDQNGASDEVL 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
            S V  L  QNG SNEGMTHEASRFKDD+VALKGERTIEALKPSDQNGAFREEVLP E S
Sbjct: 181 LSGVSTLGDQNGPSNEGMTHEASRFKDDYVALKGERTIEALKPSDQNGAFREEVLPRESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           +LKDPN TSKEE PREALKPRDQNRALKEE +SVESP K+VQNGAL+ GAY +SHLE+ V
Sbjct: 241 RLKDPNGTSKEEQPREALKPRDQNRALKEE-MSVESPKKRVQNGALESGAYETSHLEDSV 300

Query: 301 PDT----------KFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKD 360
           PDT          KFPED DE+LELNN MSR QKTAEIKQ  ELEP+ LK+AEQL++VKD
Sbjct: 301 PDTITIQPPSFVLKFPEDVDETLELNNRMSRFQKTAEIKQAAELEPVTLKRAEQLKIVKD 360

Query: 361 IDEMKSKLHEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPP 420
           IDEMKSKL+EIELKLGEAQ TISMLSEARRLSAQEVK LKEKL+ELSRRGR SNQVGFPP
Sbjct: 361 IDEMKSKLNEIELKLGEAQVTISMLSEARRLSAQEVKNLKEKLMELSRRGRVSNQVGFPP 420

Query: 421 LFICMVALICIVFGFVLHH 429
           LFICMVALICIVFGFVLHH
Sbjct: 421 LFICMVALICIVFGFVLHH 438

BLAST of Cucsat.G19868.T1 vs. NCBI nr
Match: KAA0063645.1 (vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa] >TYK18380.1 vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 703 bits (1814), Expect = 9.94e-253
Identity = 366/393 (93.13%), Postives = 375/393 (95.42%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MS+KLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSSKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPI SP DGALD RMDDVMKLNE SGPL DGMDTNE PKLKDQNG SDEV 
Sbjct: 121 KVALISPLNSPIHSPFDGALDGRMDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGTSDEVF 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PS+VPI+RYQNG SNE MT EASRFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S
Sbjct: 181 PSLVPIVRYQNGASNEDMTLEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           + KDPNETSKEELPRE LKPRDQNRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PV
Sbjct: 241 RPKDPNETSKEELPREVLKPRDQNRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPED D+SLELNNSM RLQKTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDVDKSLELNNSMPRLQKTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKL 393
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKL
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKL 393

BLAST of Cucsat.G19868.T1 vs. ExPASy TrEMBL
Match: A0A0A0LIH5 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 4.96e-303
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL
Sbjct: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS
Sbjct: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           +LKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV
Sbjct: 241 RLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420

Query: 421 IVFGFVLHH 429
           IVFGFVLHH
Sbjct: 421 IVFGFVLHH 429

BLAST of Cucsat.G19868.T1 vs. ExPASy TrEMBL
Match: A0A1S3C2B8 (vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495652 PE=4 SV=1)

HSP 1 Score: 776 bits (2005), Expect = 3.74e-282
Identity = 403/429 (93.94%), Postives = 411/429 (95.80%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPI SP DGALD RMDDVMKLNE SGPL DGMDTNE PKLKDQNGASDEVL
Sbjct: 121 KVALISPLNSPIHSPFDGALDGRMDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDEVL 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PS+VPI+RYQNG SNE MT EASRFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S
Sbjct: 181 PSLVPIVRYQNGASNEDMTLEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           + KDPNETSKEELPRE LKPRDQNRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PV
Sbjct: 241 RPKDPNETSKEELPREVLKPRDQNRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPED D+SLELNNSM RLQKTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDVDKSLELNNSMPRLQKTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGR SNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALIC 420

Query: 421 IVFGFVLHH 429
           IV GFVLHH
Sbjct: 421 IVLGFVLHH 429

BLAST of Cucsat.G19868.T1 vs. ExPASy TrEMBL
Match: A0A5D3D4C3 (Vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001030 PE=4 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 4.81e-253
Identity = 366/393 (93.13%), Postives = 375/393 (95.42%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MS+KLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSSKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPI SP DGALD RMDDVMKLNE SGPL DGMDTNE PKLKDQNG SDEV 
Sbjct: 121 KVALISPLNSPIHSPFDGALDGRMDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGTSDEVF 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
           PS+VPI+RYQNG SNE MT EASRFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S
Sbjct: 181 PSLVPIVRYQNGASNEDMTLEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           + KDPNETSKEELPRE LKPRDQNRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PV
Sbjct: 241 RPKDPNETSKEELPREVLKPRDQNRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPED D+SLELNNSM RLQKTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDVDKSLELNNSMPRLQKTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKL 393
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKL
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKL 393

BLAST of Cucsat.G19868.T1 vs. ExPASy TrEMBL
Match: A0A1S3C0N6 (vesicle-associated protein 2-2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495652 PE=4 SV=1)

HSP 1 Score: 701 bits (1808), Expect = 1.03e-252
Identity = 373/429 (86.95%), Postives = 379/429 (88.34%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180
           KVALISPLNSPI SP DGALD RMDDVMKLNE SGPL DGMDTNE PKLKDQNGASDE  
Sbjct: 121 KVALISPLNSPIHSPFDGALDGRMDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDE-- 180

Query: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240
                                            GERTIEALKPSDQNGAFREEVL HE S
Sbjct: 181 ---------------------------------GERTIEALKPSDQNGAFREEVLLHESS 240

Query: 241 KLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300
           + KDPNETSKEELPRE LKPRDQNRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PV
Sbjct: 241 RPKDPNETSKEELPREVLKPRDQNRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPV 300

Query: 301 PDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHE 360
           PDTKFPED D+SLELNNSM RLQKTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHE
Sbjct: 301 PDTKFPEDVDKSLELNNSMPRLQKTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHE 360

Query: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALIC 420
           IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGR SNQVGFPPLFICMVALIC
Sbjct: 361 IELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALIC 394

Query: 421 IVFGFVLHH 429
           IV GFVLHH
Sbjct: 421 IVLGFVLHH 394

BLAST of Cucsat.G19868.T1 vs. ExPASy TrEMBL
Match: A0A6J1G3C7 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450409 PE=4 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 7.67e-232
Identity = 357/472 (75.64%), Postives = 388/472 (82.20%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60
           MSTKLLHIQPKELQF+FELRKQSTCTVQL+NNTHHHVAFKVKTTSPKKYCVRPNVGIILP
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDMLC+DKFLIQSTIVPSG+TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPILSPSD-GALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEV 180
           KVALISPLNSP+LSP+D GAL   MD+VMKLNE SGPL DG+ TN+TPKLKDQNG+SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGTSMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LPSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEF 240
             S V  LR QNG SNEGMT EASRFKDDFVALKG RTIEALKPSDQNGA R+EVLPHE 
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SKLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSS----- 300
           S+L D N TSKEELP EALKP DQN ALKEE LSVE P K+VQNGAL W AY +S     
Sbjct: 241 SRLMDQNGTSKEELPHEALKPSDQNGALKEE-LSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 ---------------------------HLENPVPDT----------KFPEDADESLELNN 360
                                      H+E+ VP+T          KFP+  DESLELNN
Sbjct: 301 PPTVVDVQSPRPFERIFPEGGLAYKTSHVEDQVPETVAIRPPDFSVKFPDSVDESLELNN 360

Query: 361 SMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSE 420
           S+SRLQK+AEIK+  EL+ + LKKAEQL+LVKDI+EMKSKL+EIELKLGEAQGTISML E
Sbjct: 361 SVSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFE 420

Query: 421 ARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 429
           +RRLSAQEVK LKEKL+ELSR+GRASNQVGFPPLFICMVALIC+V GF+LHH
Sbjct: 421 SRRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD71.3e-5737.82Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ953.3e-3754.62Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC89.0e-3553.44Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q9LVU15.8e-3449.68Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Q84WW51.7e-3347.34Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_011648789.11.02e-30299.77vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_011648790.1 vesi... [more]
XP_008455504.17.72e-28293.94PREDICTED: vesicle-associated protein 2-2 isoform X1 [Cucumis melo][more]
XP_004139205.17.51e-26991.61vesicle-associated protein 2-2 isoform X2 [Cucumis sativus] >KAE8651570.1 hypoth... [more]
XP_038890494.17.64e-25987.02vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-... [more]
KAA0063645.19.94e-25393.13vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa] >TYK18380.1... [more]
Match NameE-valueIdentityDescription
A0A0A0LIH54.96e-30399.77MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV... [more]
A0A1S3C2B83.74e-28293.94vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349565... [more]
A0A5D3D4C34.81e-25393.13Vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C0N61.03e-25286.95vesicle-associated protein 2-2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349565... [more]
A0A6J1G3C77.67e-23275.64vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 351..371
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..279
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..428
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..111
e-value: 3.9E-31
score: 107.1
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 29.181423
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 1..128
e-value: 3.7E-42
score: 145.2
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..428
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 4..135

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19868Cucsat.G19868gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T1.E1Cucsat.G19868.T1.E1exon
Cucsat.G19868.T1.E2Cucsat.G19868.T1.E2exon
Cucsat.G19868.T1.E3Cucsat.G19868.T1.E3exon
Cucsat.G19868.T1.E4Cucsat.G19868.T1.E4exon
Cucsat.G19868.T1.E5Cucsat.G19868.T1.E5exon
Cucsat.G19868.T1.E6Cucsat.G19868.T1.E6exon
Cucsat.G19868.T1.E7Cucsat.G19868.T1.E7exon
Cucsat.G19868.T1.E8Cucsat.G19868.T1.E8exon
Cucsat.G19868.T1.E9Cucsat.G19868.T1.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T1.C1Cucsat.G19868.T1.C1CDS
Cucsat.G19868.T1.C2Cucsat.G19868.T1.C2CDS
Cucsat.G19868.T1.C3Cucsat.G19868.T1.C3CDS
Cucsat.G19868.T1.C4Cucsat.G19868.T1.C4CDS
Cucsat.G19868.T1.C5Cucsat.G19868.T1.C5CDS
Cucsat.G19868.T1.C6Cucsat.G19868.T1.C6CDS
Cucsat.G19868.T1.C7Cucsat.G19868.T1.C7CDS
Cucsat.G19868.T1.C8Cucsat.G19868.T1.C8CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T1Cucsat.G19868.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
biological_process GO:0090158 endoplasmic reticulum membrane organization
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane