Cucsat.G18632.T14 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G18632.T14
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionReplication protein A 32 kDa subunit B-like
Locationctg3379: 2603811 .. 2606219 (-)
RNA-Seq ExpressionCucsat.G18632.T14
SyntenyCucsat.G18632.T14
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGGATTTCTAGGTTTTACCAAAACAAAATTTCATTTCTCAAACTGCTCTTCAAACTGTTCTTCATCACTCACTTTGATTCTCTTGTAGGGTTCCCATATTCTTCACTCCTAGCGTTACAGAGGAAGAACGATGTATGCAAGTCAATTCGACGGCAACGCTGCTTTCTCCGGTGGTGGATTTATGCCATCTCAGACCACCCAGGCCCCTGATCATTCATTTTCTCCGGCCAAAGTACGTTCTATCTTCATATATAATTTTTATATCTCTTTCCGATTCCTCTTACTTCCTTGTTGACTGAAATTGTTACAGCAGTTTGAAAATGATTCATACTTCGATTGCTAATTAGTTGAATAATAGTTCATTTATTGTTTGGATTTGAAGTCTGTTTCGTATTTGTGTTTTCCCCCCCTGTGTTTTCGCGGTTATGGCGTTTTTCTTTCTTTCTTTATTTTTTGTCTTTAGTATATCCTTTAGAGTTTTGATTATTAACGAAATGATGACTTATATATGTGTGTGTAATAATTTGCAGAATCGGGATGTCCAGGCATTGCTTCCATTAACAGTGAAACAGATAAACGACGCATTTCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAACAATGTTAGTTTTGGACTTGTATGTTTTGTTTATTTTATGTATTGAGTTGCTTGTTGAGCTGCATTATGTTCAGGTTAAGCTTGTAGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCGTTAGACGATGGCACTGGGCGGATTGACTGTAGCAAATGGTTTGTTCTTCCCCCCACCTCCTCACCCACACCTCTACAAGATGTAGAAAAGTTCAGATTTGTTTCTTATTGATATTGTTCTTTTTTTAACAAATGTGTCTATAGGGTCAATGAAGCTGCAGATTCAAATGAAGTTGAGGGAATATTGTAATGTTCTTTCTACTCTGTCTTTAATAACTTTTAAAATATTCCCCAAGGATTATAGTACTTCTGTTGCTGTTATAATATAAAATGTTCATGATTGATTTTTCAACGTTGCCTACCAGAATCTCTTGTGAATTAGGATAGAACTTGTGGAATACACCATGTTGTAGACGTGGAGTTATACTGTTGTTCTGTAGTTTGAGAAAAATGAACTATATATCTGTAGCCTGTGCTACTACAGGGCATACAAATGCCTCGTGTTTGTCTCAGATAGCAACTATTGATGGAAAGTAGATCCTGCTTGATACTGAGAAGATGGTCGGTTGGTTTTTGTTGGGTAGAAAGGAAGCCAAGACGGAGTTGCAGAATTTTGTGGTTGGTAGTTGCTTAGGAGAGAGATCCCAGCTCTCTTGATAGCTGAAGACTTATTACTGTTTTTCTATTCGCCTTTTTTTCCTGAATTCGGACTTTGTTGCAAGTTTATCGATTTAAAAAGTGTGGTTCTTGCGTATTTTCTGGTTTGAATGCTATTTATTGAAAACAACAGGTCTCCATCAACTTGCAATTATCATTCATTGAGATGTCACTTGTGGCTCTTCTGGATTATTTTCCCTGTTTAACCTGAAATTAAATCTCTGAAACGATCATTTTGGGGACGACTCTGCTTATTTGGACTAATGATGCATTTAATCATCTCTATAATTTATCCCCTTTCAGCTCATGTCATTTAAAAATTTTCATGCTTCAGGGATGGCATGTATGTCCGGGTCCATGGCCACTTAAAGAGCTTCCAGGGTAAAAGAACTTTAAATGTCTTTTCTATCAGGTAGTGTATAGTTTTCCCTTGGTTCTTTCTGGGGATTTCTTGGTTTTGTCATTTCTTTAGGGATTTGAGTTGTGAGTGCATAAACTGTACGAATGCTGTTCTTGAACTGTAGTGGACATTAGTTTATTTTATATTTTTAGCAATTGAATTGGATGAGGTACATGAGCAGTTTTTTCAACGAAATGAAATCATTGCGGTGCTTGTTCTCTGCAACTTATGTGCATGAATTCTATACCATTGTGGCTTTGGGGTTTTGATATGAAGTATTTGGCTTGCAGGCCGGTGACAGACTATAATGAGATCACAAATCACTTCATCGAATCCATATATGTTCATTTTTACAACACCAGATTGCGGGTATTAATCAAGTCATTTCAAATTTTTAGTGATATCTTGATTAGGAAAAGTAATATTTCTTGCCTTACCTGCTTCTCCTATTGGGTTATTGTTTGTGCTGTGCTGATAGAAACAACAAAGTAGTAGTATGACTACTCAGCCACAGATGACAAATCTATCTAATACGCCCATGAAAGTATATCAAGCTCCCATTGCAAATCAAGTAAGATCCTTATTCAATTCACGTGTGGTGGGCTACGGTTTACTTATGCAACCTTCTAATGAGCTGTTT

Coding sequence (CDS)

ATGTATGCAAGTCAATTCGACGGCAACGCTGCTTTCTCCGGTGGTGGATTTATGCCATCTCAGACCACCCAGGCCCCTGATCATTCATTTTCTCCGGCCAAAAATCGGGATGTCCAGGCATTGCTTCCATTAACAGTGAAACAGATAAACGACGCATTTCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAACAATGTTAAGCTTGTAGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCGTTAGACGATGGCACTGGGCGGATTGACTGTAGCAAATGGGTCAATGAAGCTGCAGATTCAAATGAAGTTGAGGGAATATTGGATGGCATGTATGTCCGGGTCCATGGCCACTTAAAGAGCTTCCAGGGTAAAAGAACTTTAAATGTCTTTTCTATCAGGCCGGTGACAGACTATAATGAGATCACAAATCACTTCATCGAATCCATATATGTTCATTTTTACAACACCAGATTGCGGAAACAACAAAGTAGTAGTATGACTACTCAGCCACAGATGACAAATCTATCTAATACGCCCATGAAAGTATATCAAGCTCCCATTGCAAATCAAGTAAGATCCTTATTCAATTCACGTGTGGTGGGCTACGGTTTACTTATGCAACCTTCTAATGAGCTGTTT

Protein sequence

MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKSNFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTPMKVYQAPIANQVRSLFNSRVVGYGLLMQPSNELF
Homology
[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 5.3e-55
Identity = 112/215 (52.09%), Postives = 144/215 (66.98%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY   FDGNAAF+GGGFMPSQ T     S S  KNRDV+ LLPLT+KQ++ A  S+  +S
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSA--STTGES 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NF IDGVD+  V +VG +     RIT V F +DDGTG +DC +W +   ++ E+E +  G
Sbjct: 61  NFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLR--KQQSSSMT 180
           MYVR+HGHLK FQGKR++NVFS+RPVTD+NEI +HF E +YVH YNT+LR       + T
Sbjct: 121 MYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTAT 180

Query: 181 TQPQMT-NLSNTPMKVYQAPIANQVRSLFNSRVVG 213
            +PQM  +   TP K YQ   +NQ  + FN  + G
Sbjct: 181 PRPQMPYSTMPTPAKPYQTGPSNQFPNQFNDSMHG 213

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 9.8e-49
Identity = 101/210 (48.10%), Postives = 140/210 (66.67%), Query Frame = 0 

Query: 3   ASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKSNF 62
           +SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI + F SS +KS  
Sbjct: 4   SSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEKSGL 63

Query: 63  VIDGVDVNNVKLVGMVRNR-AGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGM 122
           VI+G+ + NV LVG+V ++   ++T+V F LDDGTGRIDC +WV+E  D+ E+E + DG 
Sbjct: 64  VINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVRDGT 123

Query: 123 YVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQP 182
           YVR+ GHLK+FQGK  L VFS+RP+ D+NE+T H+IE I+ +  N+  ++QQ   +T   
Sbjct: 124 YVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVTQSV 183

Query: 183 QMT--NLSNTPMKVYQAPIANQVRSLFNSR 210
             T    SNT       P+ +   +  N R
Sbjct: 184 NTTFQGGSNTNQATLLNPVVSSQNNDGNGR 213

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.8e-43
Identity = 92/216 (42.59%), Postives = 139/216 (64.35%), Query Frame = 0 

Query: 8   GNAA--FSGGGFMPSQTTQAPDHSFSPA----KNRDVQALLPLTVKQINDAFLSSDDKSN 67
           GNA+  F GGGFMPSQ T A + +        K+R+ QALLPLTVKQI DA  ++DDKSN
Sbjct: 19  GNASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDASQTNDDKSN 78

Query: 68  FVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGM 127
           F ++G++V+ V+LVG + N+  R+TDV+F LDDGTGR+  ++W N++ D+ E+  I +G 
Sbjct: 79  FAVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGD 138

Query: 128 YVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQP 187
           YV V+G LK FQGKR +  +S+R +T++N++T+HF+  ++VH   TR + Q +++  T  
Sbjct: 139 YVIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQVNANTATGT 198

Query: 188 QMTNLSNTPMKVYQAPIANQVRSLFNSRVVGYGLLM 218
               +    M   Q+P+ NQ  +    +  G G  M
Sbjct: 199 PNQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNM 234

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.2e-33
Identity = 89/226 (39.38%), Postives = 130/226 (57.52%), Query Frame = 0 

Query: 11  AFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDA---FLSSDDKSNFVIDGV 70
           AFS   F  SQ   A   S +P+K+R   + +PLTVKQI++A    ++ +  + FV+DGV
Sbjct: 9   AFSPSQFTSSQNAAA--DSTTPSKSRGASSTMPLTVKQISEAQQSGITGEKGAPFVVDGV 68

Query: 71  DVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHG 130
           +  NV+LVG+V  +  R TDV+F +DDGTGR+D  +WVN+ ADS E   + +GMYV V G
Sbjct: 69  ETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGMYVSVIG 128

Query: 131 HLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLS 190
            LK  Q ++    F+IRPVTDYNE+T HFI+ + +H  NT  + Q  S   T   M + S
Sbjct: 129 SLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENT--KSQIGSPAKTYSAMGSSS 188

Query: 191 N-------TPMKVYQAP-----IANQVRSLFNSRVVGYGLLMQPSN 222
           +       TP  V   P     + N  ++  N+ V+   +  +P+N
Sbjct: 189 SNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTEVL--NVFREPAN 228

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match: Q5Z8L1 (Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2C PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 7.0e-23
Identity = 72/211 (34.12%), Postives = 113/211 (53.55%), Query Frame = 0 

Query: 12  FSGGGFMPSQTTQAPDHSF-----------SPAKNRD--VQALLPLTVKQINDAF---LS 71
           FSG   MPSQ + AP   +           SP+K RD      +P TV  I+ +F   L+
Sbjct: 8   FSGTALMPSQRSGAPAPEYSAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALA 67

Query: 72  SDDKSN--FVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNE 131
           +D   +  F IDGV+  NV+++G V +   R TDV F LDD TG+I   +W+ + +D+ +
Sbjct: 68  ADGGGDPVFSIDGVETTNVRVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRD 127

Query: 132 VEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHF--YNTRLRK 191
              I +G+YV+V  +L  FQ K+     SIRP+ ++NE+  HFIE ++VH     +++++
Sbjct: 128 TSYIQEGVYVKVQVNLMGFQAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQR 187

Query: 192 QQSSSMTTQPQMTNLSNTPMKVYQAPIANQV 203
           Q   S+ T       S+  ++ YQ     QV
Sbjct: 188 QLPPSVQTNEYTHVPSSGGVRDYQVHFTPQV 218

[Close]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match: XP_011656845.1 (replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypothetical protein Csa_019848 [Cucumis sativus])

HSP 1 Score: 404 bits (1038), Expect = 1.60e-140
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQMTNLSNTPMKVYQAPIANQ
Sbjct: 181 PQMTNLSNTPMKVYQAPIANQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match: XP_008453602.1 (PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453603.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_016901361.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >KAA0058271.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa] >TYK11823.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 400 bits (1027), Expect = 7.60e-139
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQMTNLSNTP+K YQAPIANQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match: KAG7023385.1 (Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384 bits (985), Expect = 1.89e-132
Identity = 189/201 (94.03%), Postives = 194/201 (96.52%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY SQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQM NLSNTP++ YQ PI+NQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match: XP_023516911.1 (replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516912.1 replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 384 bits (985), Expect = 1.89e-132
Identity = 189/201 (94.03%), Postives = 194/201 (96.52%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY SQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQM NLSNTP++ YQ PI+NQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match: XP_022988280.1 (replication protein A 32 kDa subunit B [Cucurbita maxima])

HSP 1 Score: 382 bits (982), Expect = 5.41e-132
Identity = 188/201 (93.53%), Postives = 194/201 (96.52%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY SQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQM NLSNTP++ YQ P++NQ
Sbjct: 181 PQMANLSNTPVRGYQTPMSNQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match: A0A0A0LU56 (OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G423180 PE=4 SV=1)

HSP 1 Score: 404 bits (1038), Expect = 7.89e-142
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQMTNLSNTPMKVYQAPIANQ
Sbjct: 181 PQMTNLSNTPMKVYQAPIANQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match: A0A1S4DZE3 (replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494262 PE=3 SV=1)

HSP 1 Score: 400 bits (1027), Expect = 3.68e-139
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQMTNLSNTP+K YQAPIANQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match: A0A5D3CIS3 (Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00360 PE=3 SV=1)

HSP 1 Score: 400 bits (1027), Expect = 3.68e-139
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQMTNLSNTP+K YQAPIANQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match: A0A6J1JJ51 (replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC111485578 PE=3 SV=1)

HSP 1 Score: 382 bits (982), Expect = 2.62e-132
Identity = 188/201 (93.53%), Postives = 194/201 (96.52%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY SQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQM NLSNTP++ YQ P++NQ
Sbjct: 181 PQMANLSNTPVRGYQTPMSNQ 201

[Close]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match: A0A6J1E347 (replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 382 bits (981), Expect = 3.72e-132
Identity = 188/201 (93.53%), Postives = 194/201 (96.52%), Query Frame = 0 

Query: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60
           MY SQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQINDAFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120
           NFV+DGVDVNNVKLVGMVRNRAGRITDVTFALDDGTG+IDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGILDG 120

Query: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 181 PQMTNLSNTPMKVYQAPIANQ 201
           PQM NLSNTP++ YQ PI+NQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQ 201

The following BLAST results are available for this feature:
BLAST of Cucsat.G18632.T14 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LFJ85.3e-5552.09Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ... [more]
Q9ZQ199.8e-4948.10Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ... [more]
Q6H7J52.8e-4342.59Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6K9U21.2e-3339.38Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5Z8L17.0e-2334.12Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Cucsat.G18632.T14 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011656845.11.60e-140100.00replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypotheti... [more]
XP_008453602.17.60e-13999.00PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453... [more]
KAG7023385.11.89e-13294.03Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023516911.11.89e-13294.03replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022988280.15.41e-13293.53replication protein A 32 kDa subunit B [Cucurbita maxima][more]
BLAST of Cucsat.G18632.T14 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LU567.89e-142100.00OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G423180 PE=4 SV=... [more]
A0A1S4DZE33.68e-13999.00replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A5D3CIS33.68e-13999.00Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1JJ512.62e-13293.53replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC1114855... [more]
A0A6J1E3473.72e-13293.53replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014646Replication factor A protein 2PIRSFPIRSF036949RPA32coord: 1..220
e-value: 1.6E-61
score: 205.8
NoneNo IPR availableGENE3D2.40.50.140coord: 33..168
e-value: 1.8E-41
score: 142.7
NoneNo IPR availablePANTHERPTHR13989:SF42REPLICATION PROTEIN A 32 KDA SUBUNIT A-LIKEcoord: 4..206
NoneNo IPR availableCDDcd04478RPA2_DBD_Dcoord: 71..164
e-value: 1.34783E-40
score: 132.338
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 72..145
e-value: 5.5E-9
score: 35.9
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 4..206
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 41..168

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G18632Cucsat.G18632gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G18632.T14.E1Cucsat.G18632.T14.E1exon
Cucsat.G18632.T14.E2Cucsat.G18632.T14.E2exon
Cucsat.G18632.T14.E3Cucsat.G18632.T14.E3exon
Cucsat.G18632.T14.E4Cucsat.G18632.T14.E4exon
Cucsat.G18632.T14.E5Cucsat.G18632.T14.E5exon
Cucsat.G18632.T14.E6Cucsat.G18632.T14.E6exon
Cucsat.G18632.T14.E7Cucsat.G18632.T14.E7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G18632.T14.C7Cucsat.G18632.T14.C7CDS
Cucsat.G18632.T14.C6Cucsat.G18632.T14.C6CDS
Cucsat.G18632.T14.C5Cucsat.G18632.T14.C5CDS
Cucsat.G18632.T14.C4Cucsat.G18632.T14.C4CDS
Cucsat.G18632.T14.C3Cucsat.G18632.T14.C3CDS
Cucsat.G18632.T14.C2Cucsat.G18632.T14.C2CDS
Cucsat.G18632.T14.C1Cucsat.G18632.T14.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G18632.T14Cucsat.G18632.T14-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006260 DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006289 nucleotide-excision repair
cellular_component GO:0005634 nucleus
cellular_component GO:0000781 chromosome, telomeric region
cellular_component GO:0005662 DNA replication factor A complex
cellular_component GO:0035861 site of double-strand break
molecular_function GO:0003677 DNA binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003697 single-stranded DNA binding