Cucsat.G18632 (gene) Cucumber (B10) v3

Overview
NameCucsat.G18632
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionReplication protein A 32 kDa subunit B-like
Locationctg3379: 2603857 .. 2606220 (-)
RNA-Seq ExpressionCucsat.G18632
SyntenyCucsat.G18632
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACAAAATTTCATTTCTCAAACTGCTCTTCAAACTGTTCTTCATCACTCACTTTGATTCTCTTGTAGGGTTCCCATATTCTTCACTCCTAGCGTTACAGAGGAAGAACGATGTATGCAAGTCAATTCGACGGCAACGCTGCTTTCTCCGGTGGTGGATTTATGCCATCTCAGACCACCCAGGCCCCTGATCATTCATTTTCTCCGGCCAAAGTACGTTCTATCTTCATATATAATTTTTATATCTCTTTCCGATTCCTCTTACTTCCTTGTTGACTGAAATTGTTACAGCAGTTTGAAAATGATTCATACTTCGATTGCTAATTAGTTGAATAATAGTTCATTTATTGTTTGGATTTGAAGTCTGTTTCGTATTTGTGTTTTCCCCCCCTGTGTTTTCGCGGTTATGGCGTTTTTCTTTCTTTCTTTATTTTTTGTCTTTAGTATATCCTTTAGAGTTTTGATTATTAACGAAATGATGACTTATATATGTGTGTGTAATAATTTGCAGAATCGGGATGTCCAGGCATTGCTTCCATTAACAGTGAAACAGATAAACGACGCATTTCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAACAATGTTAGTTTTGGACTTGTATGTTTTGTTTATTTTATGTATTGAGTTGCTTGTTGAGCTGCATTATGTTCAGGTTAAGCTTGTAGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCGTTAGACGATGGCACTGGGCGGATTGACTGTAGCAAATGGTTTGTTCTTCCCCCCACCTCCTCACCCACACCTCTACAAGATGTAGAAAAGTTCAGATTTGTTTCTTATTGATATTGTTCTTTTTTTAACAAATGTGTCTATAGGGTCAATGAAGCTGCAGATTCAAATGAAGTTGAGGGAATATTGTAATGTTCTTTCTACTCTGTCTTTAATAACTTTTAAAATATTCCCCAAGGATTATAGTACTTCTGTTGCTGTTATAATATAAAATGTTCATGATTGATTTTTCAACGTTGCCTACCAGAATCTCTTGTGAATTAGGATAGAACTTGTGGAATACACCATGTTGTAGACGTGGAGTTATACTGTTGTTCTGTAGTTTGAGAAAAATGAACTATATATCTGTAGCCTGTGCTACTACAGGGCATACAAATGCCTCGTGTTTGTCTCAGATAGCAACTATTGATGGAAAGTAGATCCTGCTTGATACTGAGAAGATGGTCGGTTGGTTTTTGTTGGGTAGAAAGGAAGCCAAGACGGAGTTGCAGAATTTTGTGGTTGGTAGTTGCTTAGGAGAGAGATCCCAGCTCTCTTGATAGCTGAAGACTTATTACTGTTTTTCTATTCGCCTTTTTTTCCTGAATTCGGACTTTGTTGCAAGTTTATCGATTTAAAAAGTGTGGTTCTTGCGTATTTTCTGGTTTGAATGCTATTTATTGAAAACAACAGGTCTCCATCAACTTGCAATTATCATTCATTGAGATGTCACTTGTGGCTCTTCTGGATTATTTTCCCTGTTTAACCTGAAATTAAATCTCTGAAACGATCATTTTGGGGACGACTCTGCTTATTTGGACTAATGATGCATTTAATCATCTCTATAATTTATCCCCTTTCAGCTCATGTCATTTAAAAATTTTCATGCTTCAGGGATGGCATGTATGTCCGGGTCCATGGCCACTTAAAGAGCTTCCAGGGTAAAAGAACTTTAAATGTCTTTTCTATCAGGTAGTGTATAGTTTTCCCTTGGTTCTTTCTGGGGATTTCTTGGTTTTGTCATTTCTTTAGGGATTTGAGTTGTGAGTGCATAAACTGTACGAATGCTGTTCTTGAACTGTAGTGGACATTAGTTTATTTTATATTTTTAGCAATTGAATTGGATGAGGTACATGAGCAGTTTTTTCAACGAAATGAAATCATTGCGGTGCTTGTTCTCTGCAACTTATGTGCATGAATTCTATACCATTGTGGCTTTGGGGTTTTGATATGAAGTATTTGGCTTGCAGGCCGGTGACAGACTATAATGAGATCACAAATCACTTCATCGAATCCATATATGTTCATTTTTACAACACCAGATTGCGGGTATTAATCAAGTCATTTCAAATTTTTAGTGATATCTTGATTAGGAAAAGTAATATTTCTTGCCTTACCTGCTTCTCCTATTGGGTTATTGTTTGTGCTGTGCTGATAGAAACAACAAAGTAGTAGTATGACTACTCAGCCACAGATGACAAATCTATCTAATACGCCCATGAAAGTATATCAAGCTCCCATTGCAAATCAAGTAAGATCCTTATTCAATTCACGTGTGGTGGGCTACGGTTTACTTATGCAACCTTCTAATGAGCTGTTTTCAGTACACTGGTCAAGCAGGCGGTGATAGTTGGAAGAGCCTTGAGCAGATGGTCTTAGATTTTTTGCAACTTCCTTCATGCGAGTAAGTCCCTTTTTAACAATGACGCAGATCTTTTAATTTCGTCCTCCCTATTTTCATTTTCGTGCTTTTTTGTCTTGAATTTGTAATCTAGATGCATAGTTAAGAAAATAGATGCTTTCTAAGTTGTTTTCTTTCCCTCACATATGTATATCTTTTTGGTTTTGATGGCTGATGAATGTTTTTTTTTTCTCATCCGTTTTTCTCTCTTTCATGAATTGATCACTAGCTTCTTGAGACTCGACCTCCATAGCTTGGTGAATTGTGATTATACTTGTTAGGATCCTTAAATGACTCCAAGCACCAACAGGAAAAGGTTTCTCCTGGATGAGAATTATTCGGAAGAGCTGTAGTCCTAATCTAATAAAATGGTTTTTCCTTTTTCTGGAGTAGAGGAGCAGTATATCTAAGTACAGGATTGTACAACATAAACAAAGTCTAGAACGCTTCTCTCACTAGTTTCTTTCCTCATCGATTTCCTAGTTTAGATTTATTGTTACAGTCTATACATTTATTTTGTATGTAGTTCTGAAAGGGGCGCACACCGTGATGTCATTGCTCAACAGCTCAAAGTTCCACTAGAGAAGCTCATGTATTACTCTCTTTTTCACTTCTACTCGACAGTAAAACTTATCTTGCAAGTAAGGATTGTAACTTAATATTTACCATTTTGGTTATTATTTTGCAGTCCGGCGATGAAAAATCTAGAAGAGGAAGGCCTAATTTACTCAACAACTGATGATTTCCACTTCAAATCAACCGCCAACGGTTGACATTTTTGTTATGGTAACTAAACGAATTAAAGGGAAGTTTTTTTCCTGCCGTTGTTTCGTTTCATCTATAGTTTAAGTTGGGTAAATCACTATGGTCAAAATATGATCCTGAAACATCTGTTTTAGCCTTTATAACAGCGATCCAAAATCCCACATGGCTATGCCTTGTTTATAGTTGATGCAATTATTGTGATGTTTGTTTGTATTGTTCAACATATTTAAAGTGTGCTAATACAGCACAGAGTGCCAGGAGCCTGGAGAGGTGGATTTGGCTGACTCCAGGACTTCTTTCCAATCCGCAAACAGTAACTTTTTTCCCTTGCGACGTTAGAGGATAGAACACTGTTCATTTTTACATGCATTGCCAACTAACTCAAAACTTTTAAACCAATAGGTAAATTATAT

Coding sequence (CDS)

ATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAACAATGTTAGTTTTGGACTTGTATGTTTTGTTTATTTTATGTATTGAGTTGCTTGTTGAGCTGCATTATGTTCAGGTTAAGCTTGTAGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCGTTAGACGATGGCACTGGGCGGATTGACTGTAGCAAATGGGTCAATGAAGCTGCAGATTCAAATGAAGTTGAGGGAATATTGGATGGCATGTATGTCCGGGTCCATGGCCACTTAAAGAGCTTCCAGGGCCGGTGA

Protein sequence

MINQILLLMVLMLTMLVLDLYVLFILCIELLVELHYVQVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKSFQGR
Homology
BLAST of Cucsat.G18632 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.4e-31
Identity = 69/139 (49.64%), Postives = 92/139 (66.19%), Query Frame = 0

Query: 39  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKS 98
           V +VG +     RIT V F +DDGTG +DC +W +   ++ E+E +  GMYVR+HGHLK 
Sbjct: 70  VVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHGHLKI 129

Query: 99  FQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLR--KQQSSSMTTQPQMT-NLSN 158
           FQGKR++NVFS+RPVTD+NEI +HF E +YVH YNT+LR       + T +PQM  +   
Sbjct: 130 FQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQMPYSTMP 189

Query: 159 TPMKVYQAPIANQVRSLFN 175
           TP K YQ   +NQ  + FN
Sbjct: 190 TPAKPYQTGPSNQFPNQFN 208

BLAST of Cucsat.G18632 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.1e-26
Identity = 62/138 (44.93%), Postives = 89/138 (64.49%), Query Frame = 0

Query: 39  VKLVGMVRNR-AGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 98
           V LVG+V ++   ++T+V F LDDGTGRIDC +WV+E  D+ E+E + DG YVR+ GHLK
Sbjct: 73  VSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVRDGTYVRLSGHLK 132

Query: 99  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 158
           +FQGK  L VFS+RP+ D+NE+T H+IE I+ +  N+  ++QQ   +T     T    + 
Sbjct: 133 TFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVTQSVNTTFQGGS- 192

Query: 159 MKVYQAPIANQVRSLFNS 176
               QA + N V S  N+
Sbjct: 193 -NTNQATLLNPVVSSQNN 208

BLAST of Cucsat.G18632 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.9e-24
Identity = 52/130 (40.00%), Postives = 85/130 (65.38%), Query Frame = 0

Query: 39  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKS 98
           V+LVG + N+  R+TDV+F LDDGTGR+  ++W N++ D+ E+  I +G YV V+G LK 
Sbjct: 89  VRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGDYVIVNGGLKG 148

Query: 99  FQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTPM 158
           FQGKR +  +S+R +T++N++T+HF+  ++VH   TR + Q +++  T      +    M
Sbjct: 149 FQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQVNANTATGTPNQTMPRDSM 208

Query: 159 KVYQAPIANQ 169
              Q+P+ NQ
Sbjct: 209 AYNQSPLTNQ 218

BLAST of Cucsat.G18632 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.5e-23
Identity = 50/97 (51.55%), Postives = 68/97 (70.10%), Query Frame = 0

Query: 39  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKS 98
           V+LVG+V  +  R TDV+F +DDGTGR+D  +WVN+ ADS E   + +GMYV V G LK 
Sbjct: 71  VRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGMYVSVIGSLKG 130

Query: 99  FQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTR 136
            Q ++    F+IRPVTDYNE+T HFI+ + +H  NT+
Sbjct: 131 LQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTK 167

BLAST of Cucsat.G18632 vs. ExPASy Swiss-Prot
Match: Q5Z8L1 (Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2C PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 4.2e-15
Identity = 45/133 (33.83%), Postives = 76/133 (57.14%), Query Frame = 0

Query: 39  VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKS 98
           V+++G V +   R TDV F LDD TG+I   +W+ + +D+ +   I +G+YV+V  +L  
Sbjct: 86  VRVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRDTSYIQEGVYVKVQVNLMG 145

Query: 99  FQGKRTLNVFSIRPVTDYNEITNHFIESIYVHF--YNTRLRKQQSSSMTTQPQMTNLSNT 158
           FQ K+     SIRP+ ++NE+  HFIE ++VH     +++++Q   S+ T       S+ 
Sbjct: 146 FQAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQRQLPPSVQTNEYTHVPSSG 205

Query: 159 PMKVYQAPIANQV 170
            ++ YQ     QV
Sbjct: 206 GVRDYQVHFTPQV 218

BLAST of Cucsat.G18632 vs. NCBI nr
Match: XP_011656845.1 (replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypothetical protein Csa_019848 [Cucumis sativus])

HSP 1 Score: 264 bits (675), Expect = 3.79e-86
Identity = 130/131 (99.24%), Postives = 130/131 (99.24%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 190

Query: 158 MKVYQAPIANQ 168
           MKVYQAPIANQ
Sbjct: 191 MKVYQAPIANQ 201

BLAST of Cucsat.G18632 vs. NCBI nr
Match: XP_008453602.1 (PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453603.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_016901361.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >KAA0058271.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa] >TYK11823.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 260 bits (664), Expect = 1.77e-84
Identity = 128/131 (97.71%), Postives = 129/131 (98.47%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 190

Query: 158 MKVYQAPIANQ 168
           +K YQAPIANQ
Sbjct: 191 VKGYQAPIANQ 201

BLAST of Cucsat.G18632 vs. NCBI nr
Match: KAG7023385.1 (Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250 bits (638), Expect = 1.57e-80
Identity = 122/131 (93.13%), Postives = 126/131 (96.18%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT QPQM NLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANLSNTP 190

Query: 158 MKVYQAPIANQ 168
           ++ YQ PI+NQ
Sbjct: 191 VRGYQTPISNQ 201

BLAST of Cucsat.G18632 vs. NCBI nr
Match: XP_023516911.1 (replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516912.1 replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250 bits (638), Expect = 1.57e-80
Identity = 122/131 (93.13%), Postives = 126/131 (96.18%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT QPQM NLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANLSNTP 190

Query: 158 MKVYQAPIANQ 168
           ++ YQ PI+NQ
Sbjct: 191 VRGYQTPISNQ 201

BLAST of Cucsat.G18632 vs. NCBI nr
Match: XP_022988280.1 (replication protein A 32 kDa subunit B [Cucurbita maxima])

HSP 1 Score: 249 bits (635), Expect = 4.48e-80
Identity = 121/131 (92.37%), Postives = 126/131 (96.18%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT QPQM NLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANLSNTP 190

Query: 158 MKVYQAPIANQ 168
           ++ YQ P++NQ
Sbjct: 191 VRGYQTPMSNQ 201

BLAST of Cucsat.G18632 vs. ExPASy TrEMBL
Match: A0A0A0LU56 (OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G423180 PE=4 SV=1)

HSP 1 Score: 264 bits (675), Expect = 2.20e-87
Identity = 130/131 (99.24%), Postives = 130/131 (99.24%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 190

Query: 158 MKVYQAPIANQ 168
           MKVYQAPIANQ
Sbjct: 191 MKVYQAPIANQ 201

BLAST of Cucsat.G18632 vs. ExPASy TrEMBL
Match: A0A1S4DZE3 (replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494262 PE=3 SV=1)

HSP 1 Score: 260 bits (664), Expect = 8.58e-85
Identity = 128/131 (97.71%), Postives = 129/131 (98.47%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 190

Query: 158 MKVYQAPIANQ 168
           +K YQAPIANQ
Sbjct: 191 VKGYQAPIANQ 201

BLAST of Cucsat.G18632 vs. ExPASy TrEMBL
Match: A0A5D3CIS3 (Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00360 PE=3 SV=1)

HSP 1 Score: 260 bits (664), Expect = 8.58e-85
Identity = 128/131 (97.71%), Postives = 129/131 (98.47%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 190

Query: 158 MKVYQAPIANQ 168
           +K YQAPIANQ
Sbjct: 191 VKGYQAPIANQ 201

BLAST of Cucsat.G18632 vs. ExPASy TrEMBL
Match: A0A6J1JJ51 (replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC111485578 PE=3 SV=1)

HSP 1 Score: 249 bits (635), Expect = 2.17e-80
Identity = 121/131 (92.37%), Postives = 126/131 (96.18%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT QPQM NLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANLSNTP 190

Query: 158 MKVYQAPIANQ 168
           ++ YQ P++NQ
Sbjct: 191 VRGYQTPMSNQ 201

BLAST of Cucsat.G18632 vs. ExPASy TrEMBL
Match: A0A6J1E347 (replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 248 bits (634), Expect = 3.07e-80
Identity = 121/131 (92.37%), Postives = 126/131 (96.18%), Query Frame = 0

Query: 38  QVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 97
            VKLVGMVRNRAGRITDVTFALDDGTG+IDCSKWVNEAADSNEVEGILDGMYVRVHGHLK
Sbjct: 71  NVKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGILDGMYVRVHGHLK 130

Query: 98  SFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQPQMTNLSNTP 157
           SFQGKRTLNVFSIRPVTDYNEIT+HFIESIYVHFYNTRLRKQQSSSMT QPQM NLSNTP
Sbjct: 131 SFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQPQMANLSNTP 190

Query: 158 MKVYQAPIANQ 168
           ++ YQ PI+NQ
Sbjct: 191 VRGYQTPISNQ 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFJ81.4e-3149.64Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ... [more]
Q9ZQ193.1e-2644.93Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ... [more]
Q6H7J52.9e-2440.00Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6K9U22.5e-2351.55Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5Z8L14.2e-1533.83Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
XP_011656845.13.79e-8699.24replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypotheti... [more]
XP_008453602.11.77e-8497.71PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453... [more]
KAG7023385.11.57e-8093.13Replication protein A 32 kDa subunit B [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_023516911.11.57e-8093.13replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022988280.14.48e-8092.37replication protein A 32 kDa subunit B [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0LU562.20e-8799.24OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G423180 PE=4 SV=... [more]
A0A1S4DZE38.58e-8597.71replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494... [more]
A0A5D3CIS38.58e-8597.71Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1JJ512.17e-8092.37replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC1114855... [more]
A0A6J1E3473.07e-8092.37replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 175..243
e-value: 7.7E-17
score: 62.7
IPR014892Replication protein A, C-terminalPFAMPF08784RPA_Ccoord: 130..235
e-value: 5.5E-16
score: 59.1
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 39..112
e-value: 6.5E-9
score: 35.7
NoneNo IPR availableGENE3D2.40.50.140coord: 31..137
e-value: 4.0E-32
score: 112.6
NoneNo IPR availablePANTHERPTHR13989:SF42REPLICATION PROTEIN A 32 KDA SUBUNIT A-LIKEcoord: 38..244
NoneNo IPR availableCDDcd04478RPA2_DBD_Dcoord: 38..131
e-value: 2.53391E-40
score: 132.338
IPR014646Replication factor A protein 2PIRSFPIRSF036949RPA32coord: 31..242
e-value: 6.4E-58
score: 193.9
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 38..244
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 35..135
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 177..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G18632.T11Cucsat.G18632.T11mRNA
Cucsat.G18632.T9Cucsat.G18632.T9mRNA
Cucsat.G18632.T4Cucsat.G18632.T4mRNA
Cucsat.G18632.T2Cucsat.G18632.T2mRNA
Cucsat.G18632.T3Cucsat.G18632.T3mRNA
Cucsat.G18632.T1Cucsat.G18632.T1mRNA
Cucsat.G18632.T12Cucsat.G18632.T12mRNA
Cucsat.G18632.T10Cucsat.G18632.T10mRNA
Cucsat.G18632.T5Cucsat.G18632.T5mRNA
Cucsat.G18632.T6Cucsat.G18632.T6mRNA
Cucsat.G18632.T7Cucsat.G18632.T7mRNA
Cucsat.G18632.T14Cucsat.G18632.T14mRNA
Cucsat.G18632.T13Cucsat.G18632.T13mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006260 DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006289 nucleotide-excision repair
cellular_component GO:0000781 chromosome, telomeric region
cellular_component GO:0005662 DNA replication factor A complex
cellular_component GO:0005634 nucleus
cellular_component GO:0035861 site of double-strand break
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003676 nucleic acid binding