Cucsat.G18215.T3 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G18215.T3
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg3345: 2957540 .. 2961817 (-)
RNA-Seq Expression	Cucsat.G18215.T3
Synteny	Cucsat.G18215.T3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTTTATTTAAATCCAGTGTAGAAGTAAGATGATCTTTGTCTGTTTACTAAAAGAAAATTGTGGGGGTTTGAAAGATTTTAACGAGAAAATGGCAAAAAAAAAAAAAACGTAGTTAAGAAAAAGATGAATATTTTAATCTCTGCTGGTTTTTGTTGAAAATTTGAAAATAACCCATTTACGTGTGTTGAACAAGACTGTTGCTTTTTGGGGTCGTGAGAGAAAAGATGGAAAGAAAATAAGAGGGTCTGTAGATCTAATTAAACCATCAAAAGATACCAACATTCTACCATACTCTCTCAACCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTTGGGCGACCAGTTTTGGTGTGATTTCTTCAATCTTATAAATTCATCAAAACCAATGATTGATTGCAATTAGCTTCAACTCCGAGTATTATTAGTATTCAATCCTTCACGAACAATCGTTGGCGTACATTTGTGGTAGAAATAGGGATCTGGGTATACCATAACATCGTCTGATTTTGCCGACATTCTTGTTCTTTGTGGTTTATTGTTGTAAAATCGCATCTGGGTACGAGTGGTTCTTTGATTTTCTGTGATAGATTGCTATAAACATTGAGTTTTTTTTTTTTTTACAATCATTTCGATTTGCGTTTTGGGTTTTCAATCTCCCTGTTTGTAGTGATGGCGGGGAATGGGTTGTTTTACCCGATTCTTGGATTCGCATCCTGTTTGGCTTTCATTTACTTGTCGTTTGGAGATTTGAGGATTGGGGACACTAAAGTAATCCCCATAGTAGATTTTGTAGAGAGGAACGGAACGCAGTTCATGTTGAACGGGAAGGCGTTTTATATTAATGGATGGAATTCTTATTGGTTTATGGACCATTCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCCATGCTGCAGGCGGCGAGAAAGATGGGGCTTACTGTTTGCCGGACTTGGGCTTTCAATGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGAAGGTTTAACGAGCGAGTCTTTAAGGTATTGATTGAGCTGCATTTTCTGAGATTTCTGCTGTTTCTGTGATTGGATTCTTCATTTATTTGATTCTACTCTTAATGCTTTGGTTTTGTGTTCGTAATTTGTTTCTTTTTCTGAAATTTTATGGCAATGTCTGTTTTAGAGAGAGATGAGTGATTCTGCAGGGTTTGAAAGGTTCCATTGGCCAAAGAATGCAAAGCTTGGATGCTTCCACCGACAGAAAAACTGTGTCGGTAGTCATAAAACAATATGGAATTATTTTGTGAATCGCATGATTTTCTTGGAAAAGAAACGGTAGTTCTTGCCATTATGGTTAAGATGAATGAATTAGACTGAAGACAATAAATTATCTGTCAGTTCCTTTTGGCATTTAAAGGTGGATTAATTGAGTATGATTTAAATTCTCATGTTCTGTAACCCCAATAAGAAGCATTATAACTTGTATTTCTGTGGAAGATCAATCTGGTTTTGTTTGTTATCTTGTCAATATAGTTTCAAGTTCAAAAAATGAAGACTGGAATTCATGGAAGAACCAGAAAACTTTCTATTTCCATCTATCTCGCTTTCTCTTTTTGAGAAATATAAGGATAAATGACACATTATCCAAAAAATACACAAGAAAGCATATGTTTGTATATATTTTTTCCATAGCAATTGTTTAGTTTGATGGCGTTTTTTATTTGCGCAAATGCATATGTGAAGTTGTGCTGTTATCAGCTTACGTTTCAATTGGTCCTTCCTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTTCTTAGCTTAGTTAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTTTATGATCCATCAATACGCATTTATTTTAAAAATTATCTCAAGGTACATTCCTTATTTATCAATGAGTTTGTCCAATATGTTTCATAACATTGATGATTTGGTATGCATCCTGAGCTATGCCATGGATTCAAATATATTGATCCAATTGCTATTTACCATTGCATAAGCCATTAGTTCTCGGGTTCAAACAAAAATTCTTACCCTTGTCTTTGCAGATCTTACATTGCTAAATTCTACCTTATGGTGTTTTAGTGGTTCTAATGTGCACTATATTTTTCACTTTGTCAGACCGTCCTGACAAGAAAGAATTCTATCACTGGAATTGAATATAGAAATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCAGGCGATACACTTCAAGTAAGTTTTAAGATTTTGGTTCTTCTGATTTCTGAATGCTGAACCAAATGAGAATTGAAATAAATGGATTCTGGTGTACTCCCCAATTAAGTTGTAAGATTTTGATTCTTCTTGCTTCTAAATGTTAAACCAAAGGAAAACTAACCCTCATTGAACTTACTGTACTCCCGTTTGAGTCTTTTGATAGCTCACTTTTGTAATTTGGGATTGTTACGCAACTTTCTTTCTTCCAAACTTTTTCTTGTGAAAGTTATGAGCTCTCTACTTGGTATTTCTCAACCTCAACTTTGCTTCAAGTTGATTTCTCTAAAGCAAAGTTGCGTTTATTTTTCACCATGTTTTAAGTGTATTTTACCAGAATTAACTCAACGTCTAAAACGAACCTACCAGTCCCAATGTGGAATATATCTTTATCAGTTAATGGCAGAAGCAATATTCACAAATGGACTCATTTTGACCTTGTGTTTGATCCTAATATATGTTTCTCAAGATTTTTGTAAGTATGACCCAATCCTATAGACACTAATGTAGAATGACCCAATCCTAAATAATCAATGAAAACTAACCTTCAGTTGACGTCACATAGCACACTATCGCATTAGAGACATCCCTCCATTCGCAAAATAGTCGGTGCCCTAATGCAATTCATTTCAATAGCAATGTTTCTTTTTAATCAACAGTTGTCCAATCAGCAATGATCTAAAGAAACATCATATTGAAATGAATATCGGTGACTATTTTGCAATGGGTAAGACGTCTTGTTGCAAAAAGAAGTTTCGAGCAGTAGAGTGCTTTGTGATGTTAGTTGAAGGTTCGTTTTCATTGGTTTTTAGGATAGAGTCATTTCACATCAATGCTGCCACCAGGATTGGGTCATCAAATCTCTTGTTTCTCTTCCCACCCCCCACCCCCAAGGGAAGAAAAGAAAAAAGCATCGTTTCTATATCTATGCATAAGCTTATTTTGAGGAATAAAATTTGTAGGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGATGAATCTCAGTTTAAAACTTGGATGGCAATCGTCAATGATTCTACTCATGTCCATGCTCTTAAACCTGTTATTTACATAACCACATTAACATTCTGTGTCGATTAGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGATCTGACTATATCTTATTTGAGAGAATTCAATTTGTTCACTTGCTGCTATATTTTGCCTCTGTGCCCTTCGTTTCTTCTGTTGATACATATTCAGAATAGATTAGGTAGTTTTAACATATAGTATTGAGTTTCACATGAACTGCTACAAGCTGTAATGTTTTTTGAGCAAATATATAAGTCATAAATCTATTTTATTTTACTTTTCAAGT

Coding sequence (CDS)

ATGACCGATGCTTCAGGCGATACACTTCAAGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGA

Protein sequence

MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW

Homology

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BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.2e-93
Identity = 159/214 (74.30%), Postives = 184/214 (85.98%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           M+D SGDTLQ+WI EMTA+IKSID KHLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF
Sbjct: 219 MSDVSGDTLQDWINEMTAFIKSIDNKHLLTVGLEGFYGPSSPKKLTVNPERWASELGSDF 278

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           +RNS+  ++DFASVHIYPDHWFHDQ FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGL
Sbjct: 279 VRNSDSPNIDFASVHIYPDHWFHDQGFEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGL 338

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           S+LNK + P+QRD FY+T++DVIYKSAKR RSGAG+L WQFL+EGME  NDDFGIVPWE+
Sbjct: 339 SNLNKDYDPSQRDRFYRTIFDVIYKSAKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQ 398

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQR 215
            SI +L+IEQSCRL+RI G     K    +C+ R
Sbjct: 399 DSIQRLMIEQSCRLSRITGRHLLDKKSIEMCSHR 432

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BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 1.9e-86
Identity = 146/213 (68.54%), Postives = 179/213 (84.04%), Query Frame = 0


Query: 2   TDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFI 61
           TD SG TLQ+WI+EMT +IKSID KHLLTVGLEGFYGPNSPK  TVNPE+WAS+LG+DF+
Sbjct: 219 TDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFV 278

Query: 62  RNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLS 121
           +NS   ++DFASVHIYPDHWFH+Q FE++LKFV KWM SHIEDG KELKKPV+FTEFGLS
Sbjct: 279 QNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLS 338

Query: 122 DLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERS 181
           + NK + P+QRD FY+ ++DV+YKSAKR +SGAG+L WQ  +EGME  NDDFGIVP E+ 
Sbjct: 339 NQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQD 398

Query: 182 SIYQLIIEQSCRLARIGGDTQQLKALKYVCAQR 215
           SIY+L+IEQSCRL ++ G  ++ K LK +C+ R
Sbjct: 399 SIYKLMIEQSCRLGKVTGRLKEQK-LKELCSHR 430

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BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-66
Identity = 121/214 (56.54%), Postives = 158/214 (73.83%), Query Frame = 0


Query: 2   TDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGP-NSPKKSTVNPEEW-ASRLGSD 61
           +D SGDTLQ W+EEM+AY+KSIDKKHLLTVG EGFYGP +S +K  +NP EW  +  G+D
Sbjct: 232 SDPSGDTLQRWMEEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGAD 291

Query: 62  FIRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFG 121
           FIRNS+IQ +DFASVH+YPD+W      +++LKF+++W+ +H+EDGD EL+KPV+ TEFG
Sbjct: 292 FIRNSKIQDIDFASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFG 351

Query: 122 LSDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWE 181
           LS   +GF  A RD  Y+ VYD+++ SA+R  +  G+L WQ   EGMEE +D F IVP E
Sbjct: 352 LSHQVEGFEDAHRDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSE 411

Query: 182 RSSIYQLIIEQSCRLARIG-GDTQQLKALKYVCA 213
           R S+ +LI EQSCRLA +  G+    K LK VCA
Sbjct: 412 RPSMMRLIKEQSCRLAAVRYGEEGARKVLKTVCA 445

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 3.7e-53
Identity = 99/203 (48.77%), Postives = 137/203 (67.49%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           ++++SG  LQ WI EM AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDF
Sbjct: 228 VSNSSGPYLQAWIAEMAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDF 287

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           I+NS ++H+DFASVH YPD W      E++++++S W+ SH+ D ++ LKKPV+FTE G 
Sbjct: 288 IQNSAVEHIDFASVHAYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGY 347

Query: 121 ---SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVP 180
              SD N   T   RD   + VYD IY SA++ ++G+G+L WQ +VEG     D+F +V 
Sbjct: 348 LQHSDANSNST-VDRDIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVA 407

Query: 181 WERSSIYQLIIEQSCRLARIGGD 201
            +R S Y LI  QSCRL R+ G+
Sbjct: 408 RDRPSTYSLITNQSCRLQRLYGE 429

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 6.1e-48
Identity = 90/196 (45.92%), Postives = 125/196 (63.78%), Query Frame = 0


Query: 2   TDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFI 61
           +D SG  +Q WI EM A++KS+D+ HLL  GLEGFYG +SP+  T+NP     + G+DFI
Sbjct: 208 SDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFI 267

Query: 62  RNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLS 121
            N+ I  +DF +VH YPD WF D   + ++ F++KW+ +HI+D    L KP++  EFG S
Sbjct: 268 ANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKS 327

Query: 122 DLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERS 181
               G+TPAQRD  + TVY  IY SAKR  + AG L WQ LV G++   D +GI+  + S
Sbjct: 328 MKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSS 387

Query: 182 SIYQLIIEQSCRLARI 198
           S   +I +QS +L  I
Sbjct: 388 STVNVISQQSRKLTLI 400

[Close]

BLAST of Cucsat.G18215.T3 vs. NCBI nr
Match: XP_004139946.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical protein Csa_021081 [Cucumis sativus])

HSP 1 Score: 449 bits (1155), Expect = 3.77e-156
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. NCBI nr
Match: XP_008448293.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 441 bits (1134), Expect = 5.87e-153
Identity = 212/215 (98.60%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI HVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIPHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFT AQRDSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTHAQRDSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. NCBI nr
Match: KAA0062055.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 440 bits (1131), Expect = 1.68e-152
Identity = 211/215 (98.14%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI HVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIPHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFT AQRDSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTHAQRDSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLA+IGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLAKIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. NCBI nr
Match: XP_038902035.1 (mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida])

HSP 1 Score: 429 bits (1103), Expect = 3.02e-148
Identity = 206/215 (95.81%), Postives = 208/215 (96.74%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI +VDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGL
Sbjct: 277 IRNSEIPNVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDIEMKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFTPAQRD FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
            SIYQLIIEQSCRLARIGGD QQLKALKYVCAQRW
Sbjct: 397 QSIYQLIIEQSCRLARIGGDAQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. NCBI nr
Match: XP_023512795.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424 bits (1089), Expect = 6.86e-147
Identity = 203/211 (96.21%), Postives = 206/211 (97.63%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEM+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 169 MTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 228

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI H+DFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 229 IRNSEIPHIDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 288

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFTPAQRD FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 289 SDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 348

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVC 211
           SSIY+LIIEQSCRLARIGGD QQLKALK VC
Sbjct: 349 SSIYRLIIEQSCRLARIGGDIQQLKALKNVC 379

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 449 bits (1155), Expect = 1.82e-156
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 2.84e-153
Identity = 212/215 (98.60%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI HVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIPHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFT AQRDSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTHAQRDSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 2.84e-153
Identity = 212/215 (98.60%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI HVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIPHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFT AQRDSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTHAQRDSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL
Match: A0A5A7V836 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003960 PE=3 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 8.12e-153
Identity = 211/215 (98.14%), Postives = 212/215 (98.60%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 217 MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 276

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI HVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 277 IRNSEIPHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 336

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFT AQRDSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 337 SDLNKGFTHAQRDSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 396

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW 215
           SSIYQLIIEQSCRLA+IGGDTQQLKALKYVCAQRW
Sbjct: 397 SSIYQLIIEQSCRLAKIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL
Match: A0A6J1FWH9 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 PE=3 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 1.35e-146
Identity = 202/211 (95.73%), Postives = 206/211 (97.63%), Query Frame = 0


Query: 1   MTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 60
           MTDASGDTLQEWIEEM+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF
Sbjct: 169 MTDASGDTLQEWIEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDF 228

Query: 61  IRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 120
           IRNSEI H+DFASVHIYPDHWFHDQDFE+ELKFVSKWMLSHIEDGDKELKKPVMFTEFGL
Sbjct: 229 IRNSEIPHIDFASVHIYPDHWFHDQDFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGL 288

Query: 121 SDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWER 180
           SDLNKGFTPAQRD FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER
Sbjct: 289 SDLNKGFTPAQRDRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWER 348

Query: 181 SSIYQLIIEQSCRLARIGGDTQQLKALKYVC 211
           SSIY+LIIEQSCRLARIGGD QQLKALK VC
Sbjct: 349 SSIYRLIIEQSCRLARIGGDIQQLKALKNVC 379

The following BLAST results are available for this feature:

BLAST of Cucsat.G18215.T3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Y223	1.2e-93	74.30	Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ...	[more]
Q9M0H6	1.9e-86	68.54	Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ...	[more]
Q0JJD4	1.7e-66	56.54	Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q0DCM5	3.7e-53	48.77	Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FJZ3	6.1e-48	45.92	Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ...	[more]

BLAST of Cucsat.G18215.T3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139946.1	3.77e-156	100.00	mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical pr...	[more]
XP_008448293.1	5.87e-153	98.60	PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan ...	[more]
KAA0062055.1	1.68e-152	98.14	mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa]	[more]
XP_038902035.1	3.02e-148	95.81	mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida]	[more]
XP_023512795.1	6.86e-147	96.21	mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G18215.T3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB35	1.82e-156	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3...	[more]
A0A5D3C3G0	2.84e-153	98.60	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BJC2	2.84e-153	98.60	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV...	[more]
A0A5A7V836	8.12e-153	98.14	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1FWH9	1.35e-146	95.73	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 7..159 e-value: 1.9E-6 score: 27.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..198 e-value: 3.5E-56 score: 192.8
None	No IPR available	PANTHER	PTHR31451:SF61	BNAA09G43620D PROTEIN	coord: 1..213
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 1..213
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..182

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G18215	Cucsat.G18215	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T3.E1	Cucsat.G18215.T3.E1	exon
Cucsat.G18215.T3.E2	Cucsat.G18215.T3.E2	exon
Cucsat.G18215.T3.E3	Cucsat.G18215.T3.E3	exon
Cucsat.G18215.T3.E4	Cucsat.G18215.T3.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T3.C3	Cucsat.G18215.T3.C3	CDS
Cucsat.G18215.T3.C2	Cucsat.G18215.T3.C2	CDS
Cucsat.G18215.T3.C1	Cucsat.G18215.T3.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T3	Cucsat.G18215.T3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity