Cucsat.G17960.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G17960.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING-type domain-containing protein
Location	ctg3345: 2030269 .. 2032290 (+)
RNA-Seq Expression	Cucsat.G17960.T4
Synteny	Cucsat.G17960.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAGAAAGCAAAAGAAAAAGAGATTATTGTTATTAAAGTTTGTAAGTAAATTTTTTTTTTAGCAACTTTTAATCTACTCTCAACACAAAATATTTTTAAATTTCTCTCTCCCTATAAAGTTTCATAATTATGTTGTGTGAATGATGTTGGTCCAACTCTGACAGTCATATGTAATTAATATGCCCCCCTATGCCCTTTTAATTTATTTATTTGCTCTTTCGTCCTTTTATCATTCAAAGTGGTTGGTGGCTACCTCTACCTTCACACCCTATAAACATCCATTTATATTTCCAAAATTATCAAAAGGAAATTTCATTAAATATTATGTTCCATATATCTCTAAATTTATCTACATAGGTGATTTATTTATTTATTTATTTTAAGTTGAGTAAGAAGAGAGTGATAAGGTATGGAGAAAAAAGATGAGGAGGAAAGTAGTGAATGATGTAGATGAATTAAGAATGTGGTTAATGAAAGAGTTGTGATAAAGATGATTAATTGTTGGAATGAGGAAATAAAGGGATCGGAGAGGGTAAGATGTGAAGGGATGGGGTTGTGGGTTTTATTAAAAGAAAAGAAAAAAAAGAGAAGAAGTGGTTGAACTAAAATAGAGTTTGAAAGAAGGCATTAAAGGGGAAAAAAACCATATTTAAAAAAAAAAGAAGAAAGAAAGTGAGAAGTCGGTTAGACCGTACCCAAAAAAAAGAAAAAAAAAAGAAAAAGAAAAAAGCCCTTTCAGTCTCCTTACGTGCGTCATCTGCTTTAATACTCCACGTCGCATATTACCCACAATCTCAACTCTCTCTTCTTCCTTCATTATTATATTAATCACATTATATGTTTTTATTTAAACAACCAATCCAATATTCCGTTGGAAAAGGTCATGAATCTCGTAAATCTCCTCCTAACTTTCCCTAAATTACGGGATTGACCCGCATCCCCCTCTTCTCTTTTCCTCTTACTTCTTTTTACCTATAAATACAAACCCTCACATCATTTCTCTCTTCCGAATACACTTTTTTTTGTTCTTTAAATAAATTTCAATTTCCCAGAGCAACGCATTTCAATTTCTCTCTTTTTTTTTCCTTTTTTTGTATGTGATATGGGTATGGGATACGGATCGCCATTAACGGCCTTCAATGCTAACTCTACAGCCGTGGAGACCGCAGCTCTCTTTCCTCCATACAATTCTACTTTACCAATGGATTCCTTTCCTAAGGCCACTGCGGTGCTTCCCACTTCCACAGGTGTTGTTATGAAATCCGATAGCGGTATAACCTACAATCTTCCCATTCCGGCCAGAAAGCGACCGAGAGATCATCATCATCATCATTCTTCTACTTCTACACTAAACCGATTCGTTTCCTATCCTTCATCGCAACTTCATAATAATTCACAGAAGAATTGCGGATGTAATTTGTATTTTCTGGGTGAAGATATCTCAATTCAGATTCAACAACAACAGATGGATCTAGATCTATTGATTTCACAACATGTACGGTATAGCTTAACTTTTGAAAAATATATATTTATTTATAGATGTGTGTGAAATTAATTTCAATTTTACTTGATTTTGGGGGTTGTAATTAGCAGATGGAGAAGGTGAGAATGGAAGTGGAGGAGAAACGGAAGAGAGAGGCGAGGAGAATAATGGAGGTAATAGAGGTGGGGATGATGAGGGTATTGAGGAGTAAAGAGGAAGAGATAGAGAAGATGGGGAAATTAAATTGGGAATTGGAGGAGAGAGTGAATTGTTTGAGTATGGAGAATCAAATATGGAGAGATGTGGCAGAGACGAATGAAGCGACGGCAAATGCGTTAAGGAGGAATTTGGAGGAAGTGCTGCTTTTACAGGAGACGAAGACAACGGTGGTGGTGGTGGAGGAGGAGGTGGCAGAGTCGTGTTGTGAGGGGGGTGGTGGTGGGGCAGAGGAAGAAGATGATGAGAGGAGGATGAGGAAGAAGAAGAGGAAGCAGGAAGAGGAAAATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA

Coding sequence (CDS)

ATGGATTCCTTTCCTAAGGCCACTGCGGTGCTTCCCACTTCCACAGGTGTTGTTATGAAATCCGATAGCGGTATAACCTACAATCTTCCCATTCCGGCCAGAAAGCGACCGAGAGATCATCATCATCATCATTCTTCTACTTCTACACTAAACCGATTCGTTTCCTATCCTTCATCGCAACTTCATAATAATTCACAGAAGAATTGCGGATGTAATTTGTATTTTCTGGGTGAAGATATCTCAATTCAGATTCAACAACAACAGATGGATCTAGATCTATTGATTTCACAACATATGGAGAAGGTGAGAATGGAAGTGGAGGAGAAACGGAAGAGAGAGGCGAGGAGAATAATGGAGGTAATAGAGGTGGGGATGATGAGGGTATTGAGGAGTAAAGAGGAAGAGATAGAGAAGATGGGGAAATTAAATTGGGAATTGGAGGAGAGAGTGAATTGTTTGAGTATGGAGAATCAAATATGGAGAGATGTGGCAGAGACGAATGAAGCGACGGCAAATGCGTTAAGGAGGAATTTGGAGGAAGTGCTGCTTTTACAGGAGACGAAGACAACGGTGGTGGTGGTGGAGGAGGAGGTGGCAGAGTCGTGTTGTGAGGGGGGTGGTGGTGGGGCAGAGGAAGAAGATGATGAGAGGAGGATGAGGAAGAAGAAGAGGAAGCAGGAAGAGGAAAATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA

Protein sequence

MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEEEEEEE

Homology

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BLAST of Cucsat.G17960.T4 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.9e-32
Identity = 85/201 (42.29%), Postives = 124/201 (61.69%), Query Frame = 0


Query: 15  TGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLY 74
           + +   SD+ + +  P+ +RKR R+              V  PS+ +    QKN    L 
Sbjct: 78  SSLTFNSDNNVDFLRPVSSRKRSRE-----------ESVVLNPSAYM--QIQKNPTDPLM 137

Query: 75  FLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEE 134
           FLG+D+S  +QQ   D+D LIS H+E++RME+EEKRK + RRI+E +E G+M+ LR+K++
Sbjct: 138 FLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDD 197

Query: 135 EIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVL-LLQETKTTVVV 194
           EI  +GKLN  LEE+V  L +ENQIWRDVA++NEAT NALR NL++VL  ++  +     
Sbjct: 198 EINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPP 257

Query: 195 VEEEVAESCCEGGGGGAEEED 215
              + A+SCC     G  EE+
Sbjct: 258 TVADDAQSCCGSNDEGDSEEE 265

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BLAST of Cucsat.G17960.T4 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.7e-28
Identity = 99/238 (41.60%), Postives = 139/238 (58.40%), Query Frame = 0


Query: 11  LPTSTGVVMKSDSGITYN-LPIPARKRPRD---HHHHH------SSTSTLNRFVSYPSSQ 70
           +PT+ G      + +     P+P RKR RD    ++HH       S+S +N   +  ++ 
Sbjct: 75  IPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTT 134

Query: 71  LHNNSQKNCGCNLYFLGEDISI--QIQQQQMDLDLLISQH---MEKVRMEVEEKRKREAR 130
           L +           FLG+DI I   + QQQ ++D  +S H   ME+V+ E+EEKRKR+AR
Sbjct: 135 LFS-----------FLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQAR 194

Query: 131 RIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR 190
            IME IE G+++ LR KEEE E++GK+N  LEERV  LS+ENQIWRD+A+TNEATAN LR
Sbjct: 195 TIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLR 254

Query: 191 RNLEEVLLLQETKTTVV-----VVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEE 229
            NLE VL   +  +        + EE+ AESCC    GG  EE   RR+  ++  Q++
Sbjct: 255 TNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQDK 301

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-24
Identity = 75/198 (37.88%), Postives = 116/198 (58.59%), Query Frame = 0


Query: 20  KSDSGITYNLPIP--ARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLYFLG 79
           K++SG++YN  +P  + KR RD     S+     R V++ SS            +   + 
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSS------------SPSLIN 120

Query: 80  EDISIQIQ-QQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEI 139
            ++  QIQ QQQ ++D  ++Q  EK+R+E+E +++ + R +   ++  + + L+ K++EI
Sbjct: 121 VELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEI 180

Query: 140 EKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQETKTTVVVVEE 199
            ++  LNW L+ERV  L +ENQIWRD+A+TNEA AN LR NL++VL   ET  T   V E
Sbjct: 181 VRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVE 240

Query: 200 EVAE----SCCEGGGGGA 211
           + AE    SCC  GGG A
Sbjct: 241 DDAESSCGSCCGDGGGEA 246

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BLAST of Cucsat.G17960.T4 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-20
Identity = 57/130 (43.85%), Postives = 88/130 (67.69%), Query Frame = 0


Query: 83  QIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEIEKMGKL 142
           QIQQQ  ++D  ++Q  E +R+E+E +++ + R +   ++  +++ L++K+EEI +MGKL
Sbjct: 105 QIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKL 164

Query: 143 NWELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLL----LQETKTTVVVVEEEV 202
           NW L+ERV  L +ENQIWRD+A+TNEATAN LR NLE+VL     L   +  +V   ++ 
Sbjct: 165 NWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDA 224

Query: 203 AESC--CEGG 207
             SC  C+GG
Sbjct: 225 ESSCGSCDGG 234

[Close]

BLAST of Cucsat.G17960.T4 vs. NCBI nr
Match: XP_004145732.2 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] >KGN46823.1 hypothetical protein Csa_020941 [Cucumis sativus])

HSP 1 Score: 433 bits (1114), Expect = 4.18e-151
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0


Query: 1   MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 60
           MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ
Sbjct: 34  MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 93

Query: 61  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV 120
           LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV
Sbjct: 94  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV 153

Query: 121 IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE 180
           IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE
Sbjct: 154 IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE 213

Query: 181 VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 235
           VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE
Sbjct: 214 VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 268

[Close]

BLAST of Cucsat.G17960.T4 vs. NCBI nr
Match: XP_011656988.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 429 bits (1102), Expect = 2.90e-149
Identity = 235/236 (99.58%), Postives = 235/236 (99.58%), Query Frame = 0


Query: 1   MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 60
           MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ
Sbjct: 34  MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 93

Query: 61  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH-MEKVRMEVEEKRKREARRIME 120
           LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH MEKVRMEVEEKRKREARRIME
Sbjct: 94  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHQMEKVRMEVEEKRKREARRIME 153

Query: 121 VIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLE 180
           VIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLE
Sbjct: 154 VIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLE 213

Query: 181 EVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 235
           EVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE
Sbjct: 214 EVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 269

[Close]

BLAST of Cucsat.G17960.T4 vs. NCBI nr
Match: XP_008456341.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 382 bits (980), Expect = 1.06e-130
Identity = 211/232 (90.95%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 3   SFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHH------SSTSTLNRFVSY 62
           SFPKA  VLPTSTGVVMKSDSGITYNLPIP RKRPRDHHHHH      S+++TLNRFVSY
Sbjct: 35  SFPKAAPVLPTSTGVVMKSDSGITYNLPIP-RKRPRDHHHHHHHHPHSSTSNTLNRFVSY 94

Query: 63  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 122
           PSSQ+ NNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR
Sbjct: 95  PSSQVQNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 154

Query: 123 IMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 182
           IMEVIE+GMMR+LRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR
Sbjct: 155 IMEVIELGMMRILRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 214

Query: 183 NLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEE 228
           NLEEVLLLQE KTTVVVVEEEVAESCCEGGGGG +E DDERRMRKKKRK+EE
Sbjct: 215 NLEEVLLLQE-KTTVVVVEEEVAESCCEGGGGGTDE-DDERRMRKKKRKEEE 263

[Close]

BLAST of Cucsat.G17960.T4 vs. NCBI nr
Match: XP_008456340.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 377 bits (968), Expect = 7.35e-129
Identity = 211/233 (90.56%), Postives = 219/233 (93.99%), Query Frame = 0


Query: 3   SFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHH------SSTSTLNRFVSY 62
           SFPKA  VLPTSTGVVMKSDSGITYNLPIP RKRPRDHHHHH      S+++TLNRFVSY
Sbjct: 35  SFPKAAPVLPTSTGVVMKSDSGITYNLPIP-RKRPRDHHHHHHHHPHSSTSNTLNRFVSY 94

Query: 63  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH-MEKVRMEVEEKRKREAR 122
           PSSQ+ NNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH MEKVRMEVEEKRKREAR
Sbjct: 95  PSSQVQNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHQMEKVRMEVEEKRKREAR 154

Query: 123 RIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR 182
           RIMEVIE+GMMR+LRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR
Sbjct: 155 RIMEVIELGMMRILRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR 214

Query: 183 RNLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEE 228
           RNLEEVLLLQE KTTVVVVEEEVAESCCEGGGGG +E DDERRMRKKKRK+EE
Sbjct: 215 RNLEEVLLLQE-KTTVVVVEEEVAESCCEGGGGGTDE-DDERRMRKKKRKEEE 264

[Close]

BLAST of Cucsat.G17960.T4 vs. NCBI nr
Match: XP_038900738.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 287 bits (734), Expect = 7.48e-94
Identity = 168/234 (71.79%), Postives = 188/234 (80.34%), Query Frame = 0


Query: 1   MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 60
           MDSFP A  VLP STGVVMKSDSGITYNLPIP RKR R+HH+        NRFVSYPSS 
Sbjct: 26  MDSFPAA--VLPASTGVVMKSDSGITYNLPIP-RKRGREHHN-------SNRFVSYPSSH 85

Query: 61  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV 120
           +H  SQKNCGCNLYFLGEDISIQIQQQQ+D+D LISQHME+V+ME+EEK+KREARRI+EV
Sbjct: 86  VH--SQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEV 145

Query: 121 IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE 180
           IE+GMMR+LRSKEEEIEK+GKLNWELEERVNCLSMENQ+WRDVAETNEATANALR NLE+
Sbjct: 146 IEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQ 205

Query: 181 VLLLQETKTTVVVVEEEVAESCCE-------GGGGGAE------EEDDERRMRK 221
           VLL    +   V  EEE AESCC        GGGGG        +E++ERR +K
Sbjct: 206 VLL----QVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERRCKK 243

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL
Match: A0A0A0KBB9 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139230 PE=4 SV=1)

HSP 1 Score: 433 bits (1114), Expect = 2.02e-151
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0


Query: 1   MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 60
           MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ
Sbjct: 34  MDSFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSYPSSQ 93

Query: 61  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV 120
           LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV
Sbjct: 94  LHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEV 153

Query: 121 IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE 180
           IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE
Sbjct: 154 IEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRRNLEE 213

Query: 181 VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 235
           VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE
Sbjct: 214 VLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEEENEEEEE 268

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL
Match: A0A1S3C2Z7 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496308 PE=4 SV=1)

HSP 1 Score: 382 bits (980), Expect = 5.15e-131
Identity = 211/232 (90.95%), Postives = 219/232 (94.40%), Query Frame = 0


Query: 3   SFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHH------SSTSTLNRFVSY 62
           SFPKA  VLPTSTGVVMKSDSGITYNLPIP RKRPRDHHHHH      S+++TLNRFVSY
Sbjct: 35  SFPKAAPVLPTSTGVVMKSDSGITYNLPIP-RKRPRDHHHHHHHHPHSSTSNTLNRFVSY 94

Query: 63  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 122
           PSSQ+ NNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR
Sbjct: 95  PSSQVQNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 154

Query: 123 IMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 182
           IMEVIE+GMMR+LRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR
Sbjct: 155 IMEVIELGMMRILRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 214

Query: 183 NLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEE 228
           NLEEVLLLQE KTTVVVVEEEVAESCCEGGGGG +E DDERRMRKKKRK+EE
Sbjct: 215 NLEEVLLLQE-KTTVVVVEEEVAESCCEGGGGGTDE-DDERRMRKKKRKEEE 263

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL
Match: A0A1S3C331 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496308 PE=4 SV=1)

HSP 1 Score: 377 bits (968), Expect = 3.56e-129
Identity = 211/233 (90.56%), Postives = 219/233 (93.99%), Query Frame = 0


Query: 3   SFPKATAVLPTSTGVVMKSDSGITYNLPIPARKRPRDHHHHH------SSTSTLNRFVSY 62
           SFPKA  VLPTSTGVVMKSDSGITYNLPIP RKRPRDHHHHH      S+++TLNRFVSY
Sbjct: 35  SFPKAAPVLPTSTGVVMKSDSGITYNLPIP-RKRPRDHHHHHHHHPHSSTSNTLNRFVSY 94

Query: 63  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH-MEKVRMEVEEKRKREAR 122
           PSSQ+ NNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQH MEKVRMEVEEKRKREAR
Sbjct: 95  PSSQVQNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHQMEKVRMEVEEKRKREAR 154

Query: 123 RIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR 182
           RIMEVIE+GMMR+LRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR
Sbjct: 155 RIMEVIELGMMRILRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALR 214

Query: 183 RNLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDERRMRKKKRKQEE 228
           RNLEEVLLLQE KTTVVVVEEEVAESCCEGGGGG +E DDERRMRKKKRK+EE
Sbjct: 215 RNLEEVLLLQE-KTTVVVVEEEVAESCCEGGGGGTDE-DDERRMRKKKRKEEE 264

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 275 bits (702), Expect = 4.95e-89
Identity = 168/239 (70.29%), Postives = 186/239 (77.82%), Query Frame = 0


Query: 1   MDSFPKATAVLPTS----TGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSY 60
           +DSFPKA   +P S    TGVVMKSDSGI+YNLPIP RKR RDH       ST+NRF+SY
Sbjct: 30  IDSFPKALGAMPASASASTGVVMKSDSGISYNLPIP-RKRGRDH-------STINRFLSY 89

Query: 61  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 120
           PSS  H  SQKNCG N YFLGEDISI +QQQQ+DL+ LISQHMEKVRME+EEKRKREARR
Sbjct: 90  PSSHAH--SQKNCGRNFYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEEKRKREARR 149

Query: 121 IMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 180
           IME IEVGMM +LRSKEEEIEKMGKLNWELEERVN L MENQIWRDVAETNEATANALR 
Sbjct: 150 IMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNEATANALRS 209

Query: 181 NLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDE------RRMRKKKRKQEEE 229
           +LE VLL  + K    V EEE AESCC   GG  EEE++E      R+ RK +RK++EE
Sbjct: 210 DLELVLL--DVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVEEERRKGRKTRRKEKEE 256

[Close]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL
Match: A0A6J1FUW1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 274 bits (701), Expect = 7.02e-89
Identity = 168/239 (70.29%), Postives = 186/239 (77.82%), Query Frame = 0


Query: 1   MDSFPKATAVLPTS----TGVVMKSDSGITYNLPIPARKRPRDHHHHHSSTSTLNRFVSY 60
           +DSFPKA   +P S    TGVVMKSDS I+YNLPIP RKR RDH       ST+NRF+SY
Sbjct: 30  IDSFPKAVGAMPASASASTGVVMKSDSAISYNLPIP-RKRGRDH-------STINRFLSY 89

Query: 61  PSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQMDLDLLISQHMEKVRMEVEEKRKREARR 120
           PSS  H  SQKNCG NLYFLGEDISI +QQQQ+DL+ LISQHMEKVRME+EEKRKREARR
Sbjct: 90  PSSHAH--SQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEEKRKREARR 149

Query: 121 IMEVIEVGMMRVLRSKEEEIEKMGKLNWELEERVNCLSMENQIWRDVAETNEATANALRR 180
           IME IEVGMM +LRSKEEEIEKMGKLNWELEERVN L MENQIWRDVAETNEATANALR 
Sbjct: 150 IMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNEATANALRS 209

Query: 181 NLEEVLLLQETKTTVVVVEEEVAESCCEGGGGGAEEEDDE------RRMRKKKRKQEEE 229
           +LE VLL  + K    V EEE AESCC   GG  EEE++E      R+ RK +RK++EE
Sbjct: 210 DLELVLL--DVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEVEEERRKGRKTRRKEKEE 256

The following BLAST results are available for this feature:

BLAST of Cucsat.G17960.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDD1	8.9e-32	42.29	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
F4IDI6	2.7e-28	41.60	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
O81851	1.8e-24	37.88	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9FHE4	2.7e-20	43.85	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]

BLAST of Cucsat.G17960.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145732.2	4.18e-151	100.00	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] ...	[more]
XP_011656988.1	2.90e-149	99.58	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus]	[more]
XP_008456341.1	1.06e-130	90.95	PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumi...	[more]
XP_008456340.1	7.35e-129	90.56	PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumi...	[more]
XP_038900738.1	7.48e-94	71.79	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Benincasa hispida...	[more]

BLAST of Cucsat.G17960.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBB9	2.02e-151	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139230 P...	[more]
A0A1S3C2Z7	5.15e-131	90.95	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX...	[more]
A0A1S3C331	3.56e-129	90.56	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX...	[more]
A0A6J1JCV5	4.95e-89	70.29	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim...	[more]
A0A6J1FUW1	7.02e-89	70.29	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 212..240
None	No IPR available	COILS	Coil	Coil	coord: 129..149
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..240
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 211..229
None	No IPR available	PANTHER	PTHR42647:SF12	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 10..224
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 10..224

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G17960	Cucsat.G17960	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G17960.T4.E1	Cucsat.G17960.T4.E1	exon
Cucsat.G17960.T4.E2	Cucsat.G17960.T4.E2	exon
Cucsat.G17960.T4.E3	Cucsat.G17960.T4.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G17960.T4.C1	Cucsat.G17960.T4.C1	CDS
Cucsat.G17960.T4.C2	Cucsat.G17960.T4.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G17960.T4	Cucsat.G17960.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0043067	regulation of programmed cell death
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity