Cucsat.G17957.T7 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G17957.T7
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPyridoxal 5'-phosphate synthase subunit PdxT
Locationctg3345: 1955210 .. 1957994 (+)
RNA-Seq ExpressionCucsat.G17957.T7
SyntenyCucsat.G17957.T7
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGCATCTTTTTGAAAGATACAATACAATTTGCCAAAAGTGGGGATTGCGAGCCGCCAGCCTCGGCTGCAGCGAGGCTTTGTTCCAATTACCGCCACTCGAAATGAGAGGTTCCAAGTGTTCAATCGACACACCCTTCTGATTCTCTCTCAATTTCTCCCCCTTTTTAATTGCTTCGCCGGAGCCGGAGTTTCCGGCTTCTAGGGCTACTCAACGCTCCAATTCGCTACCATGACGATCGTCGGCGTTCTTGCTCTGCAGGGTTCTTTTAACGAACACATCGCAGGCATATTCTTCTTCTCCTTCTCCTTCTTCTTCGCTGTCGCTATCTCCTTTTTGTAGTAACTGATTATCTTTGTGTTAAGCAGCTTTGAGAAGGTTGGGAGTGAAGGGTGTAGAGATACGGAAGCCCGAGCAGCTTCTTAATGTAGCTTCTCTGATTATTCCTGGTGGGGAAAGTACCACCATGGCCAAACTTGCGGAGCTCCATAATTTGGTTTGTTCTTCTTTTCTTCCTCTAGATGTTGCATTCGTAATCGTTTTTGAAGGTTTTCTTTAGTTTGTTCCAAGCATCTATAGCTACATAAACGCTTCCACATGAATAAGCAAAAAAAGGTTCAAACATCTAGTATTCAATTTCGTGTAATAAGCAGAAATGCAATCTTTAAAACTTGGTGCGAAACTGGCTCGTTTCTACGAACCCGCTAAAGCTGTTTCTCGAATTAGATATATCAGACGTCTCAAATTTAGGAAATAATCGCTCATGTTCGTTTGGTTGAGCTCATTCTCATGTAGCTTAGAATTAGAATCGAGTGAAGTATTTATCTTTTGGTATTTTTGTATGTTTGCAGTTTCCTGCTTTGCGAGAGTTTGTTAGAATGGGGAAGCCTGTGTGGGGCACCTGTGCAGGACTTATCTTTTTGGCAAACAAGGCTACTGGTAGATTTCCTTCCTTTTTTCCTCAACATCTCAACTTTGGATGTTCTTTCCCAAACCCCACCTCCAACACATCAATAAATTATTGAAGTGTGTTCTAAACTTTATATGGGGACATGTATTTTCAGGACAGAAAACTGGTGGACAGGGACTTGTTGGAGGTCTGGATTGTACTGTTCACAGGAACTTCTTTGGCAGTCAGGTAACTCTCCTTTTGAGTTGTGATTTTGATTTTCAAGATGCATGAAAATAACTGGAGAAGAAACTTGTTTAGGGATCAAATTTGCATAAAGCTATCTCTGCTCGTGTGACATTGTAGCAGACGTGACTTTACCCTTTCAGTCATCCTACGAATTGGCGTCTAAAGTTTTTTTGAACCATTGCATGTTGGAAATATATAGCCCTCAAGAATTTGGGGAGCTAAGATCCTTGCTAATGGTCATTACGGTCCTTTGGGAATTTTGAACCACGTTACTATGAAATCTATCTGTCTATTGGCTCAACTAATGACTTTTCCCCAATGAAAAGTTTCATACTTCATTTAAAGTCCTCCGTGTAACAAGCATTGTGAGATCTGCTTAATTGAGGTTTTAGTTGAAGCATGGAGTTTTAAATGCAGGTTTCTCGATGGTTTTTTTTTTTTTTTCACAAGCTGATCCAAACACAAATATTCTAAGAATTTCTGCTTTGATGTTGATTCCGGATGTTCTGTTTTCCCCTCAATTACATATTTAGCAGTGTTACTAATAAGTATAGCAAGCCCATTTAACTCAACGTTGAAGTTCACTTTATATTAATTTTCCATTTCTATAGTTCAAATTTTCCTAATTTAAGAAGATCATGATTATATAGCTTCAAAGCTTTGAGTCAGAGCTCTCGATACCAGAGCTAGTGTCTTCCGAAGGTGGTCCAGAAACATTTCGTGGAGTCTTCATCCGAGCTCCTGCAATTCTTGATGCGGGACCAGAAGTCCAAGTGCTGGCTCGCTGCCCTGTCTCTTCAAACTCTAACTTGCATTCAAGTTCCTCTGGTGAAGGCAAAAAGGTTTGTTGGTATTTTCTTCTGCTTTCACAGATCCATATCATCTGTATACCGATAGTGCCTCTATTTTGAACACCTAAATTATTATAATCTGTATGTATGAGCCGGAACTTTCAAACTGTATGTATGTTGGTTGTGAAACGGTTCAGTAGAATTATAGTTTTCACTGGCATACAGGAGTTTGCTCGTCTTGCGTATGTTTTCCTCCCACCCCTTCACATTGTGGTTTGAATATCTCAGGAAAAAGATTCAGAAAGTAAAGTGATTGTAGCAGTGAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGTATGATGTAGTTTTAACTTGAAATTCTATCATACTGTGATGATAATCTCAAGTGAATTGCAACGATGGTTATGCAGGCACAGTTACTTCCTGAAGATGGCTGGTGATGTCGAAGAATCCTCAAGTAGCATAGAAAGCATAGGAGTTAACAATTCAAGATTCGAAAGATCAAAGAGCGACCTTCCGATTTTTATTTAGTTTAAACCAATTGAACGCCTCCTCAACCTAAATCTACAGTTTCAAGTTTCATTCTAGATTTAATCTATCTATTCTGTAGTTTGCACCATGTTTTATCTTCCTTACGTTAAGTTACAACATTCTTCTACTGTGTTAAGTTTATTTTGCTATAACTTTGTGTAGGAGATGCTGTACATGGAGTCTTCATTTGAAAAGATTCATCTCCTTTCTCATATACCACTAAAATATAATTAGTCTTAAACAGGGTAAAACTTAGAATACGAATGAATGCATGGA

Coding sequence (CDS)

ATGACGATCGTCGGCGTTCTTGCTCTGCAGGGTTCTTTTAACGAACACATCGCAGCTTTGAGAAGGTTGGGAGTGAAGGGTGTAGAGATACGGAAGCCCGAGCAGCTTCTTAATGTAGCTTCTCTGATTATTCCTGGTGGGGAAAGTACCACCATGGCCAAACTTGCGGAGCTCCATAATTTGTTTCCTGCTTTGCGAGAGTTTGTTAGAATGGGGAAGCCTGTGTGGGGCACCTGTGCAGGACTTATCTTTTTGGCAAACAAGGCTACTGGACAGAAAACTGGTGGACAGGGACTTGTTGGAGGTCTGGATTGTACTGTTCACAGGAACTTCTTTGGCAGTCAGCTTCAAAGCTTTGAGTCAGAGCTCTCGATACCAGAGCTAGTGTCTTCCGAAGGTGGTCCAGAAACATTTCGTGGAGTCTTCATCCGAGCTCCTGCAATTCTTGATGCGGGACCAGAAGTCCAAGTGCTGGCTCGCTGCCCTGTCTCTTCAAACTCTAACTTGCATTCAAGTTCCTCTGGTGAAGGCAAAAAGGAAAAAGATTCAGAAAGTAAAGTGATTGTAGCAGTGAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGTTACTTCCTGAAGATGGCTGGTGATGTCGAAGAATCCTCAAGTAGCATAGAAAGCATAGGAGTTAACAATTCAAGATTCGAAAGATCAAAGAGCGACCTTCCGATTTTTATTTAG

Protein sequence

MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDSESKVIVAVRQGNLLATAFHPELTSDNRCYFLKMAGDVEESSSSIESIGVNNSRFERSKSDLPIFI
Homology
BLAST of Cucsat.G17957.T7 vs. ExPASy Swiss-Prot
Match: Q8LAD0 (Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX=3702 GN=PDX2 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 6.5e-94
Identity = 179/252 (71.03%), Postives = 212/252 (84.13%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           VGVLALQGSFNEHIAALRRLGV+GVEIRK +QLL V+SLIIPGGESTTMAKLAE HNLFP
Sbjct: 3   VGVLALQGSFNEHIAALRRLGVQGVEIRKADQLLTVSSLIIPGGESTTMAKLAEYHNLFP 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
           ALREFV+MGKPVWGTCAGLIFLA++A GQK GGQ LVGGLDCTVHRNFFGSQ+QSFE+++
Sbjct: 63  ALREFVKMGKPVWGTCAGLIFLADRAVGQKEGGQELVGGLDCTVHRNFFGSQIQSFEADI 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKD- 183
            +P+L S EGGPET+RGVFIRAPA+LD GP+V+VLA  PV SN  L+SSS+ + ++E   
Sbjct: 123 LVPQLTSQEGGPETYRGVFIRAPAVLDVGPDVEVLADYPVPSNKVLYSSSTVQIQEEDAL 182

Query: 184 SESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEE-----SSSSIESIGVNNS 243
            E+KVIVAV+QGNLLATAFHPELT+D R   YF+KM  ++E+     SS +I S+G  ++
Sbjct: 183 PETKVIVAVKQGNLLATAFHPELTADTRWHSYFIKMTKEIEQGASSSSSKTIVSVGETSA 242

Query: 244 RFERSKSDLPIF 248
             E +K DLPIF
Sbjct: 243 GPEPAKPDLPIF 254

BLAST of Cucsat.G17957.T7 vs. ExPASy Swiss-Prot
Match: Q3Z8V9 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=pdxT PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.7e-41
Identity = 101/218 (46.33%), Postives = 131/218 (60.09%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           +GVLALQG+F EHI  LR LG + VE+RK E+L  ++ LIIPGGESTT+ KL     L  
Sbjct: 3   IGVLALQGAFREHINMLRTLGAEAVEVRKAEELAELSGLIIPGGESTTITKLLYTFGLAK 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            +++  R G PVWGTCAG+I LA + +G  +G + L   +D TV RN FG Q+ SFE+ L
Sbjct: 63  PVKDLARNGMPVWGTCAGMICLAKELSGDISGVKTL-ELMDITVRRNAFGRQVDSFEAML 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            +  L   EGG   F  VFIRAP +   G  V+VLA+ P                     
Sbjct: 123 KVKAL---EGG--DFPAVFIRAPLVEKTGQWVEVLAKLP--------------------- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRC--YFLKMAGD 220
               +VAVR+ NLLAT+FHPEL++DNR   YF++MA D
Sbjct: 183 -DGTMVAVRENNLLATSFHPELSADNRFHRYFVQMAKD 192

BLAST of Cucsat.G17957.T7 vs. ExPASy Swiss-Prot
Match: A5D6D2 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=pdxT PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 8.3e-41
Identity = 103/214 (48.13%), Postives = 130/214 (60.75%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           VGVLALQG+F EH   L   G + V++RKPEQL ++++L+IPGGESTT+ KL    NLF 
Sbjct: 3   VGVLALQGAFREHQKVLAACGAESVQVRKPEQLEDISALVIPGGESTTIGKLLLEFNLFE 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            L +  + G PV+GTCAG+I LA +  G    GQ  +G +D +V RN FG Q++SFE++L
Sbjct: 63  PLVKLGQGGLPVFGTCAGMILLAREIAG---SGQPRLGLMDISVERNAFGRQVESFEADL 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            IP L     G E FR VFIRAP I++AG  V+VLAR                G+K    
Sbjct: 123 DIPVL-----GEEPFRAVFIRAPYIIEAGGGVEVLARF---------------GEK---- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRC--YFLK 216
               IV  RQG  LA AFHPELT D R   YFL+
Sbjct: 183 ----IVMARQGRCLAAAFHPELTGDLRIHRYFLE 185

BLAST of Cucsat.G17957.T7 vs. ExPASy Swiss-Prot
Match: Q2RMI9 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=pdxT PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.2e-40
Identity = 104/217 (47.93%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           +GVLA+QG+F EHI +L  LGV+GVEIR   QL  +A LIIPGGESTT+ KL    NL  
Sbjct: 3   IGVLAMQGAFREHIQSLEALGVQGVEIRHANQLEGIAGLIIPGGESTTIGKLMVEFNLLE 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            +R     G PV+GTCAG++ LA    G     Q  +G ++  V RN FG Q+ SFE +L
Sbjct: 63  PVRHLAEGGLPVFGTCAGMVLLARDIIG---SDQPRLGLMNARVQRNAFGRQVDSFEVDL 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            IP L     G E F  VFIRAP I +  P  + LA                   K+K  
Sbjct: 123 EIPVL-----GEEPFHAVFIRAPYIEEIEPPAEALATF-----------------KDK-- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAG 219
               IV VRQGNLLATAFHPELT D R   YFLKM G
Sbjct: 183 ----IVMVRQGNLLATAFHPELTKDLRVHSYFLKMIG 188

BLAST of Cucsat.G17957.T7 vs. ExPASy Swiss-Prot
Match: A7Z0D4 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) OX=326423 GN=pdxT PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.1e-40
Identity = 98/219 (44.75%), Postives = 128/219 (58.45%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M  +GVL LQG+  EHI ++   G KGV I++PEQL ++  LI+PGGESTTM +L + ++
Sbjct: 1   MLTIGVLGLQGAVREHIRSIEACGSKGVVIKRPEQLDDIDGLILPGGESTTMRRLMDTYH 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
               LREF   GKP++GTCAGLI LA +  G      GL   L+  V RN FG Q+ SFE
Sbjct: 61  FMEPLREFAAQGKPMFGTCAGLIILAKEIAGTDNAHLGL---LNVVVERNSFGRQVDSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           ++L+I      +G  E F GVFIRAP IL+AG +V+VL           H+         
Sbjct: 121 ADLTI------KGLDEPFTGVFIRAPHILEAGEDVEVLCE---------HNGR------- 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRC--YFLKMA 218
                  IVA +QGN L  +FHPELT D+R    F+KMA
Sbjct: 181 -------IVAAKQGNFLGCSFHPELTDDHRVTELFVKMA 187

BLAST of Cucsat.G17957.T7 vs. NCBI nr
Match: XP_004145736.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN46830.1 hypothetical protein Csa_020959 [Cucumis sativus])

HSP 1 Score: 471 bits (1213), Expect = 3.45e-167
Identity = 246/250 (98.40%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLARCPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSSIESIGVNNSRFE
Sbjct: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957.T7 vs. NCBI nr
Match: XP_008463934.1 (PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo])

HSP 1 Score: 466 bits (1200), Expect = 3.31e-165
Identity = 242/250 (96.80%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957.T7 vs. NCBI nr
Match: XP_038900301.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida])

HSP 1 Score: 454 bits (1169), Expect = 1.76e-160
Identity = 236/250 (94.40%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M IVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHS+SSGEGK+E
Sbjct: 121 TELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSE KVIVAVRQGNLLATAFHPELTSDNR   YFLKM GDVEESSSSI SIGVNNS FE
Sbjct: 181 KDSERKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMVGDVEESSSSIASIGVNNSTFE 240

Query: 241 RSKSDLPIFI 248
           RSK+DLPIFI
Sbjct: 241 RSKTDLPIFI 250

BLAST of Cucsat.G17957.T7 vs. NCBI nr
Match: KAA0035336.1 (putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa] >TYK14301.1 putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa])

HSP 1 Score: 442 bits (1137), Expect = 9.90e-156
Identity = 234/250 (93.60%), Postives = 237/250 (94.80%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQ        ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQ--------ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 242

BLAST of Cucsat.G17957.T7 vs. NCBI nr
Match: XP_023534477.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 429 bits (1104), Expect = 1.41e-150
Identity = 223/250 (89.20%), Postives = 234/250 (93.60%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M  VGVLALQGS+NEHIAALRRLGVK VEI+KPEQLL+VASLIIPGGESTTMAKLAELHN
Sbjct: 1   MATVGVLALQGSYNEHIAALRRLGVKSVEIKKPEQLLDVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFP+LREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPSLREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVS+EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNS LHS+SSGEGK+E
Sbjct: 121 TELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSALHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K +E KVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVE SSSSI SIGV+NSRFE
Sbjct: 181 KGAEDKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEGSSSSIASIGVDNSRFE 240

Query: 241 RSKSDLPIFI 248
           RS  DLPIF+
Sbjct: 241 RSNIDLPIFL 250

BLAST of Cucsat.G17957.T7 vs. ExPASy TrEMBL
Match: A0A0A0KAR5 (Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1)

HSP 1 Score: 471 bits (1213), Expect = 1.67e-167
Identity = 246/250 (98.40%), Postives = 246/250 (98.40%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLARCPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSSIESIGVNNSRFE
Sbjct: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957.T7 vs. ExPASy TrEMBL
Match: A0A1S3CLW1 (Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 1.60e-165
Identity = 242/250 (96.80%), Postives = 245/250 (98.00%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957.T7 vs. ExPASy TrEMBL
Match: A0A5D3CR96 (Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=3 SV=1)

HSP 1 Score: 442 bits (1137), Expect = 4.79e-156
Identity = 234/250 (93.60%), Postives = 237/250 (94.80%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQ        ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQ--------ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 242

BLAST of Cucsat.G17957.T7 vs. ExPASy TrEMBL
Match: A0A6J1H447 (Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 9.71e-151
Identity = 223/250 (89.20%), Postives = 233/250 (93.20%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M  VGVLALQGS+NEHIAALRRLGVK VEIRKPEQLL+VASLIIPGGESTTMAKLAELHN
Sbjct: 1   MATVGVLALQGSYNEHIAALRRLGVKSVEIRKPEQLLDVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFP+LREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPSLREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVS+EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNS LHS+SSGEGK+E
Sbjct: 121 TELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSTLHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K +E KVIVAVRQGNLLATAFHPELTSDNR   YFLKMAGDVE SSSSI SIGV+N RFE
Sbjct: 181 KGAEDKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEGSSSSIASIGVDNLRFE 240

Query: 241 RSKSDLPIFI 248
           RS  DLPIF+
Sbjct: 241 RSNIDLPIFL 250

BLAST of Cucsat.G17957.T7 vs. ExPASy TrEMBL
Match: A0A6J1CIP7 (Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1)

HSP 1 Score: 425 bits (1092), Expect = 4.61e-149
Identity = 220/250 (88.00%), Postives = 236/250 (94.40%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M IVGVLALQGSFNEHI+ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHISALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELS+PELV++EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSN++S+S+GE K+E
Sbjct: 121 AELSMPELVATEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNVNSNSTGEDKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNR--CYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K SESKVIVAVRQGNLLATAFHPELTSD+R   YFLKMAG+V+ SSSSI   GV+N+R E
Sbjct: 181 KGSESKVIVAVRQGNLLATAFHPELTSDSRWHSYFLKMAGEVDGSSSSIAIEGVDNARLE 240

Query: 241 RSKSDLPIFI 248
           RSKSDLPIF+
Sbjct: 241 RSKSDLPIFL 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LAD06.5e-9471.03Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX... [more]
Q3Z8V93.7e-4146.33Pyridoxal 5'-phosphate synthase subunit PdxT OS=Dehalococcoides mccartyi (strain... [more]
A5D6D28.3e-4148.13Pyridoxal 5'-phosphate synthase subunit PdxT OS=Pelotomaculum thermopropionicum ... [more]
Q2RMI93.2e-4047.93Pyridoxal 5'-phosphate synthase subunit PdxT OS=Moorella thermoacetica (strain A... [more]
A7Z0D44.1e-4044.75Pyridoxal 5'-phosphate synthase subunit PdxT OS=Bacillus velezensis (strain DSM ... [more]
Match NameE-valueIdentityDescription
XP_004145736.13.45e-16798.40probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN4683... [more]
XP_008463934.13.31e-16596.80PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo][more]
XP_038900301.11.76e-16094.40probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida][more]
KAA0035336.19.90e-15693.60putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa]... [more]
XP_023534477.11.41e-15089.20probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo... [more]
Match NameE-valueIdentityDescription
A0A0A0KAR51.67e-16798.40Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1[more]
A0A1S3CLW11.60e-16596.80Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1[more]
A0A5D3CR964.79e-15693.60Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=... [more]
A0A6J1H4479.71e-15189.20Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1[more]
A0A6J1CIP74.61e-14988.00Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOTIGRFAMTIGR03800TIGR03800coord: 4..160
e-value: 2.1E-47
score: 159.4
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPFAMPF01174SNOcoord: 6..210
e-value: 3.2E-47
score: 160.8
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPIRSFPIRSF005639Glut_amidoT_SNOcoord: 1..223
e-value: 8.8E-73
score: 242.0
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPANTHERPTHR31559PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNOcoord: 2..235
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOHAMAPMF_01615PdxTcoord: 4..216
score: 32.739376
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPROSITEPS51130PDXT_SNO_2coord: 3..218
score: 60.501694
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOCDDcd01749GATase1_PBcoord: 4..214
e-value: 6.10965E-97
score: 279.41
IPR029062Class I glutamine amidotransferase-likeGENE3D3.40.50.880coord: 1..229
e-value: 6.9E-76
score: 256.5
IPR029062Class I glutamine amidotransferase-likeSUPERFAMILY52317Class I glutamine amidotransferase-likecoord: 3..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..184
NoneNo IPR availablePANTHERPTHR31559:SF1SUBFAMILY NOT NAMEDcoord: 2..235
NoneNo IPR availablePROSITEPS51273GATASE_TYPE_1coord: 3..223
score: 10.866364
IPR021196PdxT/SNO family, conserved sitePROSITEPS01236PDXT_SNO_1coord: 41..51

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G17957Cucsat.G17957gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G17957.T7.E1Cucsat.G17957.T7.E1exon
Cucsat.G17957.T7.E2Cucsat.G17957.T7.E2exon
Cucsat.G17957.T7.E3Cucsat.G17957.T7.E3exon
Cucsat.G17957.T7.E4Cucsat.G17957.T7.E4exon
Cucsat.G17957.T7.E5Cucsat.G17957.T7.E5exon
Cucsat.G17957.T7.E6Cucsat.G17957.T7.E6exon
Cucsat.G17957.T7.E7Cucsat.G17957.T7.E7exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G17957.T7.C1Cucsat.G17957.T7.C1CDS
Cucsat.G17957.T7.C2Cucsat.G17957.T7.C2CDS
Cucsat.G17957.T7.C3Cucsat.G17957.T7.C3CDS
Cucsat.G17957.T7.C4Cucsat.G17957.T7.C4CDS
Cucsat.G17957.T7.C5Cucsat.G17957.T7.C5CDS
Cucsat.G17957.T7.C6Cucsat.G17957.T7.C6CDS
Cucsat.G17957.T7.C7Cucsat.G17957.T7.C7CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G17957.T7Cucsat.G17957.T7-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0042819 vitamin B6 biosynthetic process
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0008614 pyridoxine metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:1903600 glutaminase complex
molecular_function GO:0004359 glutaminase activity