Cucsat.G17957 (gene) Cucumber (B10) v3

Overview
NameCucsat.G17957
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPyridoxal 5'-phosphate synthase subunit PdxT
Locationctg3345: 1955141 .. 1958059 (+)
RNA-Seq ExpressionCucsat.G17957
SyntenyCucsat.G17957
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACTTTTTATAGTGTAATAACTTTACAATATAGTCAATGAAATATAGTTTTGATTGAATTTGCAAGTTGAGCATCTTTTTGAAAGATACAATACAATTTGCCAAAAGTGGGGATTGCGAGCCGCCAGCCTCGGCTGCAGCGAGGCTTTGTTCCAATTACCGCCACTCGAAATGAGAGGTTCCAAGTGTTCAATCGACACACCCTTCTGATTCTCTCTCAATTTCTCCCCCTTTTTAATTGCTTCGCCGGAGCCGGAGTTTCCGGCTTCTAGGGCTACTCAACGCTCCAATTCGCTACCATGACGATCGTCGGCGTTCTTGCTCTGCAGGGTTCTTTTAACGAACACATCGCAGGCATATTCTTCTTCTCCTTCTCCTTCTTCTTCGCTGTCGCTATCTCCTTTTTGTAGTAACTGATTATCTTTGTGTTAAGCAGCTTTGAGAAGGTTGGGAGTGAAGGGTGTAGAGATACGGAAGCCCGAGCAGCTTCTTAATGTAGCTTCTCTGATTATTCCTGGTGGGGAAAGTACCACCATGGCCAAACTTGCGGAGCTCCATAATTTGGTTTGTTCTTCTTTTCTTCCTCTAGATGTTGCATTCGTAATCGTTTTTGAAGGTTTTCTTTAGTTTGTTCCAAGCATCTATAGCTACATAAACGCTTCCACATGAATAAGCAAAAAAAGGTTCAAACATCTAGTATTCAATTTCGTGTAATAAGCAGAAATGCAATCTTTAAAACTTGGTGCGAAACTGGCTCGTTTCTACGAACCCGCTAAAGCTGTTTCTCGAATTAGATATATCAGACGTCTCAAATTTAGGAAATAATCGCTCATGTTCGTTTGGTTGAGCTCATTCTCATGTAGCTTAGAATTAGAATCGAGTGAAGTATTTATCTTTTGGTATTTTTGTATGTTTGCAGTTTCCTGCTTTGCGAGAGTTTGTTAGAATGGGGAAGCCTGTGTGGGGCACCTGTGCAGGACTTATCTTTTTGGCAAACAAGGCTACTGGTAGATTTCCTTCCTTTTTTCCTCAACATCTCAACTTTGGATGTTCTTTCCCAAACCCCACCTCCAACACATCAATAAATTATTGAAGTGTGTTCTAAACTTTATATGGGGACATGTATTTTCAGGACAGAAAACTGGTGGACAGGGACTTGTTGGAGGTCTGGATTGTACTGTTCACAGGAACTTCTTTGGCAGTCAGGTAACTCTCCTTTTGAGTTGTGATTTTGATTTTCAAGATGCATGAAAATAACTGGAGAAGAAACTTGTTTAGGGATCAAATTTGCATAAAGCTATCTCTGCTCGTGTGACATTGTAGCAGACGTGACTTTACCCTTTCAGTCATCCTACGAATTGGCGTCTAAAGTTTTTTTGAACCATTGCATGTTGGAAATATATAGCCCTCAAGAATTTGGGGAGCTAAGATCCTTGCTAATGGTCATTACGGTCCTTTGGGAATTTTGAACCACGTTACTATGAAATCTATCTGTCTATTGGCTCAACTAATGACTTTTCCCCAATGAAAAGTTTCATACTTCATTTAAAGTCCTCCGTGTAACAAGCATTGTGAGATCTGCTTAATTGAGGTTTTAGTTGAAGCATGGAGTTTTAAATGCAGGTTTCTCGATGGTTTTTTTTTTTTTTTCACAAGCTGATCCAAACACAAATATTCTAAGAATTTCTGCTTTGATGTTGATTCCGGATGTTCTGTTTTCCCCTCAATTACATATTTAGCAGTGTTACTAATAAGTATAGCAAGCCCATTTAACTCAACGTTGAAGTTCACTTTATATTAATTTTCCATTTCTATAGTTCAAATTTTCCTAATTTAAGAAGATCATGATTATATAGCTTCAAAGCTTTGAGTCAGAGCTCTCGATACCAGAGCTAGTGTCTTCCGAAGGTGGTCCAGAAACATTTCGTGGAGTCTTCATCCGAGCTCCTGCAATTCTTGATGCGGGACCAGAAGTCCAAGTGCTGGCTCGCTGCCCTGTCTCTTCAAACTCTAACTTGCATTCAAGTTCCTCTGGTGAAGGCAAAAAGGTTTGTTGGTATTTTCTTCTGCTTTCACAGATCCATATCATCTGTATACCGATAGTGCCTCTATTTTGAACACCTAAATTATTATAATCTGTATGTATGAGCCGGAACTTTCAAACTGTATGTATGTTGGTTGTGAAACGGTTCAGTAGAATTATAGTTTTCACTGGCATACAGGAGTTTGCTCGTCTTGCGTATGTTTTCCTCCCACCCCTTCACATTGTGGTTTGAATATCTCAGGAAAAAGATTCAGAAAGTAAAGTGATTGTAGCAGTGAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGTATGATGTAGTTTTAACTTGAAATTCTATCATACTGTGATGATAATCTCAAGTGAATTGCAACGATGGTTATGCAGGCACAGTTACTTCCTGAAGATGGCTGGTGATGTCGAAGAATCCTCAAGTAGCATAGAAAGCATAGGAGTTAACAATTCAAGATTCGAAAGATCAAAGAGCGACCTTCCGATTTTTATTTAGTTTAAACCAATTGAACGCCTCCTCAACCTAAATCTACAGTTTCAAGTTTCATTCTAGATTTAATCTATCTATTCTGTAGTTTGCACCATGTTTTATCTTCCTTACGTTAAGTTACAACATTCTTCTACTGTGTTAAGTTTATTTTGCTATAACTTTGTGTAGGAGATGCTGTACATGGAGTCTTCATTTGAAAAGATTCATCTCCTTTCTCATATACCACTAAAATATAATTAGTCTTAAACAGGGTAAAACTTAGAATACGAATGAATGCATGGAGGTGATTAATCTACCGAATTGTATTTAAGTTTTAAATTTGGAAAAATTTGAATAGTTAACTAAAC

Coding sequence (CDS)

ATGACGATCGTCGGCGTTCTTGCTCTGCAGGGTTCTTTTAACGAACACATCGCAGCTTTGAGAAGGTTGGGAGTGAAGGGTGTAGAGATACGGAAGCCCGAGCAGCTTCTTAATGTAGCTTCTCTGATTATTCCTGGTGGGGAAAGTACCACCATGGCCAAACTTGCGGAGCTCCATAATTTGTTTCCTGCTTTGCGAGAGTTTGTTAGAATGGGGAAGCCTGTGTGGGGCACCTGTGCAGGACTTATCTTTTTGGCAAACAAGGCTACTGGACAGAAAACTGGTGGACAGGGACTTGTTGGAGGTCTGGATTGTACTGTTCACAGGAACTTCTTTGGCAGTCAGCTTCAAAGCTTTGAGTCAGAGCTCTCGATACCAGAGCTAGTGTCTTCCGAAGGTGGTCCAGAAACATTTCGTGGAGTCTTCATCCGAGCTCCTGCAATTCTTGATGCGGGACCAGAAGTCCAAGTGCTGGCTCGCTGCCCTGTCTCTTCAAACTCTAACTTGCATTCAAGTTCCTCTGGTGAAGGCAAAAAGGAAAAAGATTCAGAAAGTAAAGTGATTGTAGCAGTGAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGCACAGTTACTTCCTGAAGATGGCTGGTGATGTCGAAGAATCCTCAAGTAGCATAGAAAGCATAGGAGTTAACAATTCAAGATTCGAAAGATCAAAGAGCGACCTTCCGATTTTTATTTAG

Protein sequence

MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFERSKSDLPIFI
Homology
BLAST of Cucsat.G17957 vs. ExPASy Swiss-Prot
Match: Q8LAD0 (Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX=3702 GN=PDX2 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 4.4e-98
Identity = 182/252 (72.22%), Postives = 215/252 (85.32%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           VGVLALQGSFNEHIAALRRLGV+GVEIRK +QLL V+SLIIPGGESTTMAKLAE HNLFP
Sbjct: 3   VGVLALQGSFNEHIAALRRLGVQGVEIRKADQLLTVSSLIIPGGESTTMAKLAEYHNLFP 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
           ALREFV+MGKPVWGTCAGLIFLA++A GQK GGQ LVGGLDCTVHRNFFGSQ+QSFE+++
Sbjct: 63  ALREFVKMGKPVWGTCAGLIFLADRAVGQKEGGQELVGGLDCTVHRNFFGSQIQSFEADI 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKD- 183
            +P+L S EGGPET+RGVFIRAPA+LD GP+V+VLA  PV SN  L+SSS+ + ++E   
Sbjct: 123 LVPQLTSQEGGPETYRGVFIRAPAVLDVGPDVEVLADYPVPSNKVLYSSSTVQIQEEDAL 182

Query: 184 SESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEE-----SSSSIESIGVNNS 243
            E+KVIVAV+QGNLLATAFHPELT+D RWHSYF+KM  ++E+     SS +I S+G  ++
Sbjct: 183 PETKVIVAVKQGNLLATAFHPELTADTRWHSYFIKMTKEIEQGASSSSSKTIVSVGETSA 242

Query: 244 RFERSKSDLPIF 250
             E +K DLPIF
Sbjct: 243 GPEPAKPDLPIF 254

BLAST of Cucsat.G17957 vs. ExPASy Swiss-Prot
Match: Q3Z8V9 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=pdxT PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.1e-43
Identity = 102/218 (46.79%), Postives = 133/218 (61.01%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           +GVLALQG+F EHI  LR LG + VE+RK E+L  ++ LIIPGGESTT+ KL     L  
Sbjct: 3   IGVLALQGAFREHINMLRTLGAEAVEVRKAEELAELSGLIIPGGESTTITKLLYTFGLAK 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            +++  R G PVWGTCAG+I LA + +G  +G + L   +D TV RN FG Q+ SFE+ L
Sbjct: 63  PVKDLARNGMPVWGTCAGMICLAKELSGDISGVKTL-ELMDITVRRNAFGRQVDSFEAML 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            +  L   EGG   F  VFIRAP +   G  V+VLA+ P                     
Sbjct: 123 KVKAL---EGG--DFPAVFIRAPLVEKTGQWVEVLAKLP--------------------- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGD 222
               +VAVR+ NLLAT+FHPEL++DNR+H YF++MA D
Sbjct: 183 -DGTMVAVRENNLLATSFHPELSADNRFHRYFVQMAKD 192

BLAST of Cucsat.G17957 vs. ExPASy Swiss-Prot
Match: A5D6D2 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=pdxT PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 6.8e-43
Identity = 104/214 (48.60%), Postives = 131/214 (61.21%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           VGVLALQG+F EH   L   G + V++RKPEQL ++++L+IPGGESTT+ KL    NLF 
Sbjct: 3   VGVLALQGAFREHQKVLAACGAESVQVRKPEQLEDISALVIPGGESTTIGKLLLEFNLFE 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            L +  + G PV+GTCAG+I LA +  G    GQ  +G +D +V RN FG Q++SFE++L
Sbjct: 63  PLVKLGQGGLPVFGTCAGMILLAREIAG---SGQPRLGLMDISVERNAFGRQVESFEADL 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            IP L     G E FR VFIRAP I++AG  V+VLAR                G+K    
Sbjct: 123 DIPVL-----GEEPFRAVFIRAPYIIEAGGGVEVLARF---------------GEK---- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLK 218
               IV  RQG  LA AFHPELT D R H YFL+
Sbjct: 183 ----IVMARQGRCLAAAFHPELTGDLRIHRYFLE 185

BLAST of Cucsat.G17957 vs. ExPASy Swiss-Prot
Match: Q2RMI9 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=pdxT PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 8.9e-43
Identity = 106/217 (48.85%), Postives = 125/217 (57.60%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           +GVLA+QG+F EHI +L  LGV+GVEIR   QL  +A LIIPGGESTT+ KL    NL  
Sbjct: 3   IGVLAMQGAFREHIQSLEALGVQGVEIRHANQLEGIAGLIIPGGESTTIGKLMVEFNLLE 62

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            +R     G PV+GTCAG++ LA    G     Q  +G ++  V RN FG Q+ SFE +L
Sbjct: 63  PVRHLAEGGLPVFGTCAGMVLLARDIIG---SDQPRLGLMNARVQRNAFGRQVDSFEVDL 122

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
            IP L     G E F  VFIRAP I +  P  + LA                   K+K  
Sbjct: 123 EIPVL-----GEEPFHAVFIRAPYIEEIEPPAEALATF-----------------KDK-- 182

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAG 221
               IV VRQGNLLATAFHPELT D R HSYFLKM G
Sbjct: 183 ----IVMVRQGNLLATAFHPELTKDLRVHSYFLKMIG 188

BLAST of Cucsat.G17957 vs. ExPASy Swiss-Prot
Match: Q6AFB8 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Leifsonia xyli subsp. xyli (strain CTCB07) OX=281090 GN=pdxT PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.5e-42
Identity = 101/213 (47.42%), Postives = 121/213 (56.81%), Query Frame = 0

Query: 4   VGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHNLFP 63
           VGVLALQG   EH+  L  LG   V +R+P++L  VA L+IPGGES+ M KLA    L  
Sbjct: 7   VGVLALQGDVREHLTVLWALGADAVRVRRPDELETVAGLVIPGGESSVMDKLARTVGLAG 66

Query: 64  ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFESEL 123
            LR  +  G PV+GTCAGLI LA+        GQ  +GGLD  V RN FGSQ +SFE++L
Sbjct: 67  PLRNAIAGGLPVYGTCAGLIMLADTIV-DGIAGQRSLGGLDVAVRRNAFGSQAESFETDL 126

Query: 124 SIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDS 183
           S P L     G E    VFIRAP +   GP   VLAR P             +G+     
Sbjct: 127 SFPTL-----GAEPMHAVFIRAPIVESVGPRATVLARVP-------------DGR----- 186

Query: 184 ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFL 217
               +VAV Q NLL TAFHPEL+ D R+H YFL
Sbjct: 187 ----VVAVEQDNLLGTAFHPELSGDKRFHEYFL 191

BLAST of Cucsat.G17957 vs. NCBI nr
Match: XP_004145736.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN46830.1 hypothetical protein Csa_020959 [Cucumis sativus])

HSP 1 Score: 486 bits (1252), Expect = 4.24e-173
Identity = 249/250 (99.60%), Postives = 249/250 (99.60%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLARCPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE
Sbjct: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957 vs. NCBI nr
Match: XP_008463934.1 (PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo])

HSP 1 Score: 481 bits (1239), Expect = 4.07e-171
Identity = 245/250 (98.00%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957 vs. NCBI nr
Match: XP_038900301.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida])

HSP 1 Score: 469 bits (1208), Expect = 2.16e-166
Identity = 239/250 (95.60%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M IVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHS+SSGEGK+E
Sbjct: 121 TELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSE KVIVAVRQGNLLATAFHPELTSDNRWHSYFLKM GDVEESSSSI SIGVNNS FE
Sbjct: 181 KDSERKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMVGDVEESSSSIASIGVNNSTFE 240

Query: 241 RSKSDLPIFI 250
           RSK+DLPIFI
Sbjct: 241 RSKTDLPIFI 250

BLAST of Cucsat.G17957 vs. NCBI nr
Match: KAA0035336.1 (putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa] >TYK14301.1 putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa])

HSP 1 Score: 457 bits (1176), Expect = 1.22e-161
Identity = 237/250 (94.80%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQ        ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQ--------ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 242

BLAST of Cucsat.G17957 vs. NCBI nr
Match: XP_023534477.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 444 bits (1143), Expect = 1.75e-156
Identity = 226/250 (90.40%), Postives = 237/250 (94.80%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M  VGVLALQGS+NEHIAALRRLGVK VEI+KPEQLL+VASLIIPGGESTTMAKLAELHN
Sbjct: 1   MATVGVLALQGSYNEHIAALRRLGVKSVEIKKPEQLLDVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFP+LREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPSLREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVS+EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNS LHS+SSGEGK+E
Sbjct: 121 TELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSALHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K +E KVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVE SSSSI SIGV+NSRFE
Sbjct: 181 KGAEDKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEGSSSSIASIGVDNSRFE 240

Query: 241 RSKSDLPIFI 250
           RS  DLPIF+
Sbjct: 241 RSNIDLPIFL 250

BLAST of Cucsat.G17957 vs. ExPASy TrEMBL
Match: A0A0A0KAR5 (Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1)

HSP 1 Score: 486 bits (1252), Expect = 2.05e-173
Identity = 249/250 (99.60%), Postives = 249/250 (99.60%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLARCPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE
Sbjct: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957 vs. ExPASy TrEMBL
Match: A0A1S3CLW1 (Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 1.97e-171
Identity = 245/250 (98.00%), Postives = 248/250 (99.20%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 250

BLAST of Cucsat.G17957 vs. ExPASy TrEMBL
Match: A0A5D3CR96 (Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=3 SV=1)

HSP 1 Score: 457 bits (1176), Expect = 5.91e-162
Identity = 237/250 (94.80%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           MTIVGVLALQ        ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MTIVGVLALQ--------ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVKMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           SELSIPELVSSEGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSNLHSSSSGEGKKE
Sbjct: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLHSSSSGEGKKE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           KD+ESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSS+ESIGVNNSRFE
Sbjct: 181 KDAESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSLESIGVNNSRFE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIFI
Sbjct: 241 RSKSDLPIFI 242

BLAST of Cucsat.G17957 vs. ExPASy TrEMBL
Match: A0A6J1H447 (Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 1.20e-156
Identity = 226/250 (90.40%), Postives = 236/250 (94.40%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M  VGVLALQGS+NEHIAALRRLGVK VEIRKPEQLL+VASLIIPGGESTTMAKLAELHN
Sbjct: 1   MATVGVLALQGSYNEHIAALRRLGVKSVEIRKPEQLLDVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFP+LREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPSLREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELSIPELVS+EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNS LHS+SSGEGK+E
Sbjct: 121 TELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSTLHSNSSGEGKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K +E KVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVE SSSSI SIGV+N RFE
Sbjct: 181 KGAEDKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEGSSSSIASIGVDNLRFE 240

Query: 241 RSKSDLPIFI 250
           RS  DLPIF+
Sbjct: 241 RSNIDLPIFL 250

BLAST of Cucsat.G17957 vs. ExPASy TrEMBL
Match: A0A6J1CIP7 (Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 5.70e-155
Identity = 223/250 (89.20%), Postives = 239/250 (95.60%), Query Frame = 0

Query: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60
           M IVGVLALQGSFNEHI+ALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHISALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQLQSFE 120
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ LVGGLDCTVHRNFFGSQLQSFE
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQLQSFE 120

Query: 121 SELSIPELVSSEGGPETFRGVFIRAPAILDAGPEVQVLARCPVSSNSNLHSSSSGEGKKE 180
           +ELS+PELV++EGGPETFRGVFIRAPAILD GPEVQVLA CPVSSNSN++S+S+GE K+E
Sbjct: 121 AELSMPELVATEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNVNSNSTGEDKEE 180

Query: 181 KDSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIESIGVNNSRFE 240
           K SESKVIVAVRQGNLLATAFHPELTSD+RWHSYFLKMAG+V+ SSSSI   GV+N+R E
Sbjct: 181 KGSESKVIVAVRQGNLLATAFHPELTSDSRWHSYFLKMAGEVDGSSSSIAIEGVDNARLE 240

Query: 241 RSKSDLPIFI 250
           RSKSDLPIF+
Sbjct: 241 RSKSDLPIFL 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LAD04.4e-9872.22Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX... [more]
Q3Z8V91.1e-4346.79Pyridoxal 5'-phosphate synthase subunit PdxT OS=Dehalococcoides mccartyi (strain... [more]
A5D6D26.8e-4348.60Pyridoxal 5'-phosphate synthase subunit PdxT OS=Pelotomaculum thermopropionicum ... [more]
Q2RMI98.9e-4348.85Pyridoxal 5'-phosphate synthase subunit PdxT OS=Moorella thermoacetica (strain A... [more]
Q6AFB81.5e-4247.42Pyridoxal 5'-phosphate synthase subunit PdxT OS=Leifsonia xyli subsp. xyli (stra... [more]
Match NameE-valueIdentityDescription
XP_004145736.14.24e-17399.60probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN4683... [more]
XP_008463934.14.07e-17198.00PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo][more]
XP_038900301.12.16e-16695.60probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida][more]
KAA0035336.11.22e-16194.80putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa]... [more]
XP_023534477.11.75e-15690.40probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo... [more]
Match NameE-valueIdentityDescription
A0A0A0KAR52.05e-17399.60Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1[more]
A0A1S3CLW11.97e-17198.00Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1[more]
A0A5D3CR965.91e-16294.80Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=... [more]
A0A6J1H4471.20e-15690.40Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1[more]
A0A6J1CIP75.70e-15589.20Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOTIGRFAMTIGR03800TIGR03800coord: 4..160
e-value: 1.0E-47
score: 160.4
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPFAMPF01174SNOcoord: 6..158
e-value: 2.2E-41
score: 141.8
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPIRSFPIRSF005639Glut_amidoT_SNOcoord: 1..186
e-value: 5.5E-61
score: 203.4
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPANTHERPTHR31559PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNOcoord: 2..179
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPROSITEPS51130PDXT_SNO_2coord: 3..196
score: 49.966351
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOCDDcd01749GATase1_PBcoord: 4..160
e-value: 1.47909E-79
score: 233.186
IPR029062Class I glutamine amidotransferase-likeGENE3D3.40.50.880coord: 1..182
e-value: 6.4E-61
score: 207.6
IPR029062Class I glutamine amidotransferase-likeSUPERFAMILY52317Class I glutamine amidotransferase-likecoord: 3..159
NoneNo IPR availablePANTHERPTHR31559:SF1SUBFAMILY NOT NAMEDcoord: 2..179
NoneNo IPR availablePROSITEPS51273GATASE_TYPE_1coord: 3..152
score: 9.585632
IPR021196PdxT/SNO family, conserved sitePROSITEPS01236PDXT_SNO_1coord: 41..51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G17957.T1Cucsat.G17957.T1mRNA
Cucsat.G17957.T3Cucsat.G17957.T3mRNA
Cucsat.G17957.T2Cucsat.G17957.T2mRNA
Cucsat.G17957.T4Cucsat.G17957.T4mRNA
Cucsat.G17957.T5Cucsat.G17957.T5mRNA
Cucsat.G17957.T7Cucsat.G17957.T7mRNA
Cucsat.G17957.T6Cucsat.G17957.T6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008614 pyridoxine metabolic process
biological_process GO:0042819 vitamin B6 biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:1903600 glutaminase complex
molecular_function GO:0004359 glutaminase activity