Cucsat.G1706.T4 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G1706.T4
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionautophagy-related protein 13b
Locationctg1002: 631169 .. 639055 (-)
RNA-Seq ExpressionCucsat.G1706.T4
SyntenyCucsat.G1706.T4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAGGGGAAAAAGGGTGTGGGGATTTGGCAATGGCATTCAAAACCAGATAGCCCCAACACGTTCCTTCCTCAATACTGCACTCCTTAACACTGGTGGATCCACGCTTCGAAGTTCGACCCTTCCCTCTCTCTCCAATTGCTTTCTTTCACCTTTCAACTTTGAATTTCCACCTTCTGATCATCATCAATCACAGCGTATTACCTTAATTCAACTTTTTTTTTTTTTTTTAGTTTTTTGTTTTGTGTTGATTTTCCTTCATGGGCGATTCCAATTTCATCACTTTTTCCTTCTTTCTCATTTGTTCTCAAACGGGGTTTCTTTACTAATTTGATTCTCCCCATGTCCATGATCTTTCTTCCGTTGCAGTGAATTGCAGTCTAGTGGGTCTCCGAACGCTTCAATTTGGGGTTTTCCGGTAAAGGGTCTGTTTCAATTTTACTCATTTTTGTTGTCGTTGTTGTCCGTATGGCTTCTTCTCATAATACTCATTCCGAGACTGCAAAAGTTGAGCAAATTTTAACTGAATTCTTTCCTAAAACGCTACAAATTATACTGGAATCGAGGGCCCCTTGTATTTCATCGCGTAATTTTAGTGGCGAACAAGTTTTGTCTTCTCCATCTTCTTCCTCCTCGTCTTCCTCGAGTATGAGGCCGAGGGATAAATGGTTTAATTTGGCTCTTCGTGAATGCTCTGCAACGTTAGAGAATATTGATCTCTGGCGTCCGAATTATCATGAACCTATGGTTGTTGATGTTATTTTGGTGCAGAGGCAATTTGGTTTAGATTCTGTTAGTGCTTCTCCTAGGAAAGATCTTGTTAGGAATTTGTCTCTGAAAGAGAAATATCCACTCTCTTTCAATTCTGATAAGGATGAGTTTGGAAGTCAGACCAAGAGTGAGAAAGTTGTAGAGCGATGGATGGTGCATTATGAGAGTAGGAAAAACCGGGATAGTAATTCGGGTAGCAGAAGGTCCAGTAATAGTACTGCTCACACCTACAAGAAAACCATTTTACTCTTAAGGTCTTTATATGCCTTTGTGAGACTTTTACCTGCCTATAAGGTTTTTCAAGATATCAGTTCATCTGGTCAGATTCACCCTTTTACTCTTGCTCATCGTGTGTCTTCCTTTGCTGAGCCTTTCACCCGGAGGGAAGAGGCCGAAATGCAGCGATTTGTGTTCACTCCTGTCGACACTTCTTGTGGTCGACTATGCCTCTCAGTGTTATACCGTTCATCCCTCTCTGACATAAACTCTGAACCATCAACTCCTATGTCTCCTCAAGTTATTCCTGAATATGTTGGGAGTCCATTGGCAGATCCATTGAAAAGGTTTCCTACCCTCCCTGTAACGATAGCACCATCTCATGGATCTCCATCGTCGTTGCCATTCTCAAGACGTCATAGTTGGAGTTATGACCGTTTTAGACCTTCCCCTCCTTCAGTCTCCTTTTCACCTTCACCAACACATTCAGAGTCTCATGCTTTAATTTCAAATCCAGCCTTTCCCCGTTTACCTCCTTCAAGCTTACCTTCACAACTGCCTGAAATGGTTACGGGACATAAAGATAATATGAACTACGATGAATATTACCCTTCTCCTGTCTTTTCTCAATCACCCTCACTGTCACCACCAATTCGCATTCGTGTAAAGCGCCTGCCAAATGGTCTATTGCAGTCTGAAAGTGCTCCTCCAAGTGCACCTATTGCAAAGCTTCCTCATTCCCCTGCATTATCAAGCAAACCGAATTTGCCACCATCCCCTCCCCTTAAAGCTTCTGGAGCAATTATTTCTAGGATTAATAGAGATGTTGGTCCATTGCCGGCTGGCTCTGCAATTGGAAAGGTATATTTCTGCTTATTGTTATTTCCCACCTCAAATGTGTTATGTTCAATATCTTGATTCTTTTTTGTCTTCTTTAGTCATCTTCTCTTGGACGAGATGAAAGCCAAAGAATTTCAGGCTGGAGGGTATCATCCAATAACTCACCAATTTCAAGAAGCTCTAGTAGATCTTTTCCAGATGACTTAGATGATCCAGAGTTTCCATGCCCCTTTGATGTGGATGAGGATGAGATGACAGATAGAGGTAGCAGGTAATGACTGCTTACATTCTTCTCTTTTCGTGACAATAATAATTAAATTTGCAGAATTTATTTTCCTGCTTATGTTTGAGATCATTATTTGTTGAAGGTAGAGATTTAAATGGCAACATAACAAAAAATCTTGCTGCAACTTTTCTACTTTAAACACTAGTTACTTTAGTTTAGTAGATTAAGTTACTCTTGATTATTTCACATACACTCTAAAGGCCCCTTTCTAGTTAGGACAGAAAACAAGAGATTTGCAAGGCTGCTTTACAATTCTCCTTGGGATGGGGGGTGGGTTAGGCTCTTCGAATCTGAGGAATTTTATGGACTTTCTTTTGAATAAAATACTTATCAATCTAGGAGGACAAAAGCACCAATTAGACGGTCCAAAACCACTTTCGGGGAAGAAAACCTAAAAAATTCATCCATGCACATCAAAAGTCTACTTGTAATTGTTGTTATTATTTTTATTCTTTTTTATGAGAAACTATTCTACTTGTTATTGTTTATACCATTTCATTATGAGGTACATCGAATCATCGAAGTCCGTCTAGTTTTCAAGAACCCATACAAATCAGTCTAAAATTAAAATTTCCCTGAGTCAATGTTAACATATGCTCAAAAGCTGAAAACCTGAGTGAAGACAAGACGCATGAGCAACATTGACAATTCTAGTGATTTGGGTGGCTATCAATTGGTTCAAACCAACTAGCCTTGGATGAAGAGCTTTATAAGTTTCTAACAAAAACTTATTTTCTCACTTCAAACTTTCCCCATGATGAGTTACCTTAAGGAATCCATTTAAGCGATGCTGGTAATGAAGGTAACTTTGGAAGGTAAAACAAAAATGGGGGAAGAATATGGTTTTAAAGCTCTTCATGGGAGAAATATGGAAGATCGTTATTTTTCAATTTTCAAGATGGTCAATAAGTTGTGCGTGCTCAGGCACACACTTATCAGGGATAGAGAAAACCCCCTTCGAAAAATTACTTGACTTTTGGCTTCCCGTCGTTGCTAATCATGACGAGGCTGTGGTATAGAATTGTTGGCAGCCAGGTAGCCTTCTCAAAGGGTGACCGCTGGTTTTTTCTTTTTTTTTCTCTTCTTCTTCTTCTTCTTCCTTTCCACAGTTAGCTGAAAATAGTCCATTGGTACCAGGTCATTGTTATTTGGAAAGGATCTGAAATTGAGAAAAGGACTATTAGGGGGGTGTTTGGCACTCAAGTTGAGTTGAGTTGGGTGGGTAAAATAACCCACCCACCCACAGTTTGGAAGTACCAATAATTCAAGAGGTTTATTACCTCATTGTTTGCGTTAACTCACAATTCTCACCTTATGAACTCACATTTCTTATTTCGTTATTCGTGTCAACTCTTCCAATTACTCACTCCAATAATAATTACCAACTCAACTCAACTCTCCCGAAAAAACTCAAACCAAACACCACCTTAGAGATGTGTAGTATAGGAAGAAAATGAAGAATATCTTGGTAATGATGAATTTGATGAAAATTAAGATTAAAATGACGATTTAGAAAAGCTTAGAAATTAGTTGTAATGAGCAATTGTTATGGAAAGATATCCGTGCTTATTGCATGTTCCAGAGGGAAAACAAGCATGATAATAGAAATGAATCAAAATATATGAAGCAAAACTTACTGCCAGTTAATATAGTGAGTGCAAAGCAGATGAGGATAAGAATGAGACAAATTGGGATGGGTATGGACATTGGAGCTAGAACTTACTGGTGAGGTGCTGAGGATTGATGTTGTACAGATAAAATTGAGGTGAGGGAAGCACGTCAGAAGGCTGGAGTTCGATAATGATAGGTAGGAGAATGCTTGAGAAATTGACAAAAGTATATCAGAAACCCATTGTGGTTGTTGAAGAACACATAAATATGGGGCTTTGTGCTTGGTGAATGAATGGTGCATCTTGATGTAATTTTTTCTGCTTTTATTTTTTTTAAAATAAAAGACAACCTATCACGTTTGAGTTTCTTTTTCTAGTTATTTTTAGTTTATGATTATCTTTAATTTGTGGGTCTTATTGGTTAGGTAGCTCGTAGCTGGTTTGCTTAGTGCTCTTACATTCTTTTTAATCAGAAGGATCAAATCTCTTGTATTAGTAAGTTCTTTTCTAATACCGAATTGGTCTGTAACCTTGATGGTTCATTTGGTTGGTTGGCTTTATTTGCCACTCCTGGTTCGAATCTCGTAATCGGTATTCAGTTTGAATTTGTCAGTAAGTTCTTTTCTACAATACTAAACAACTTTTGGTTAATAGTTATTGGAGATTTCTTTCCCTAATTCTAGGTTCTTTCACGAACATACTAGCCTAATCCATTGCCATTCCATCGTGGTTGGATGGTATTAGACAAAACATAGGCAGGATCTCAAGGTTTTTGACAATGGAGATTTTGATTCTAGGAATAGTGGGAATGAGCACTGTAACATTGGAGCATTTTAGGCTTTTAATGGAAATTCAAATATAAGTATGGTGGGGGAGGGAAATTTTTTTATTTAGCCAATGCATGCTTTAAAATTCGAAGGGAGGATGGACTAAAACAAGTAAGGATGTGGTTGGACTTCAAAATGTGCTGCAACAATAGTGCGAAAGCTGTAAGGTAGAAGGGGAAGGCATATGCATTGGTTCAAAACAGTTCTGTAAAGGGTTGGAAGCTGGGGTTTATTTCAAAGTTCCTTTTGAAGTGCTCCAGTAGACGAGGTAGTTGGTTAATTGAGAGGCGGTAATGGAGTTTTTGCTTTAAATGGCTTACGAAATTTGAGAAGCAGACGTCTGAGGTTCTTTTGCTTCTTTACCACCAGGAAAAAATGACAAAGGAATCACTCTTGGTCATGAGATTGTCTAGATCTCTGAGAAATGAAGCTATAAAATTTTTGCTTGTATAAACAGAACTTGGATGGAGGAGATCGACGGATACTAAAGACGAGGAGATGCTGACTGACTGGTTTTTTAAAAGAGAAAAGGGACAGAAATTTTTTTGTACCTTAACTATAGGGGAGGAGAGGATTGATGATTGAGTTTCGTACTCATATTGAAAATGGTGAGGTTTCTAGAGTGTGTCTAAATTGATAATATAACAGGATGAGCTATCAAGGATAAGGCATGATCTACTGTTTGTTATCCAAGAAACAATATTCTTTCAAAGGATTCCTGTTATAGAGAATTGGTTTCATTGCATATGTACGGAACACTATCAAACTTCTGTTGAATGGATACTGAAAAGCTGTCTTATATATATGAATCTTCAAATTAGTTGGAACCTAACCCTTAACCTAAACCCTTAATTGCAGAACTTTCATAATGAAAATTTGATGGACTGCTAAGCTGTCATGGTTTATACCATTTACCTATCTCAAAGTTGTTCATAATTTGAGAAGCTGATGTAGGAAGGTTATTCAATTTAGGTTCCTTTGGTCATGATCTGTTAAATTATCAACCAACCTAAAAGATTAAGTTCATGGGTTAAAAACTTACAATTATTGCTATCAATACTTTTAGCACACCCTGACATGCGGGTTTGAAAATTTGTAGGTCTAACAAGTAAAAATCAATATTAATCAAGGAGCAAATGAAATTATAGGAATTTTACATAAGACCCTTATTCTGGTATCAGGTTGAATCACCAATCAAAACAAAAATTTAATTTGGCCCATTAAAGTATTTTAACTATTTTAAAACTCTTGTTCTTTAATTTCTTGGGAAGAGGCTCTCTCAATCCTTTGCCCTTAGGTTGTTCTTTTTTGCTTTTGTACTAATATATTATTATGTTTCTTATTAAAGAAAAAAAATTGAGTACAATGAGAGTGGGAGATTTAATAAGGAGCTACTAGAGAATTGACCCGATGCCTCTACTGATTTAAGAAAGAAATCAAAATCCCTAAAAACTCTGTTCATCTATTTGCTGTAATAATCCATCTATATCCAAGATCCGTTGGACCATGTTCAATTAAGCCTTAAAGAGTGAGAGCTCTCATGAGAACATCTCTTGCCACTCCAAGAACCCTCCTAGGAAGAAGGTAGGCGTAGGAACAATTGGAGCAACACACTATGTTGAAAAGAATAAGAAACAGTCGGTAATCTTAAAGACTTGAAACTGGATATCACACTGACGAACGACTATTCATATAGTTAGCTTGGATTTTTAACTTTTATATATTTTTTAGTTACAATGGAATTGATGTATTCATTTCATGTGGTGATTGTTAGCATCTATATTCTGCTTCTAAGTGGATAATAAATTAGGGATGTATCTTTTATTGGTTTATATTACACCAATTCAAATCTCAACCGCAGATCCACCATGCTTCTCTGGTTGAATTACTGTTATTAAAGTCTTCTGCAGCCGCATAACATCTTCCAAACTAGTCAAACTCTGGAAAAATACATTCCTTCAACGATAATGAGAATCGTTACTTACTATATTAAACTTCTCCTGTTCCCTGTTTGATCTTGTTCAAAGCCAGTTCTCCAATCCCCTATTGAATCAGGGAAAGTTGGTTTATGAATCAAGTACAGTAGCCATTTTCTGCCCCACCAACAGATTAACTTTTCTTTTACAGCCTTCTCGCCATTAGAGAATTAGATGCTTCCCTGAGGAATTAATTTTTTAAAACCATGATTTTGGAACTTGAGGAAGTGAAGTGAACATGGCTGTTAAATGTGTAGCAGCGGAGCATAATTTTCCTTTTAGAAAATACTTAGACTTTCATCGAGAAATGTCCGTAAGAGGGGGGGTCCAAATCAAAGGAAAAGCTCTAGTTAAGAGAAATAAGACTAAGCAAACGATTACAAAAGTGCTTACACGCATAGGTCCGTGAAACCAAAAGGAATGTAATAAAGGACCACACATCGCCACAACAACTCTCGAGTAATAGTAGCAGGACATAATTTCCTTCGTTTACTTTCCCTAAACTGATATTTCCTTCATGTTTTTATCTTGTGACGTATCTATCATATACCCAAATTGTTATATGTTATATGAATTGTCTTATTAAACCTTTATTTTAAATCTCTCCTAGGCCTGAATCCTTCGACCAAAAAGGAAATCTTTGCGACATGCTGGAACCCGGGGGATTTTTTCCGATCCGTAAATCTCAAGATGCTGCTGTTGGTGCTCTAGTACGTATGCTTCAAAAAGCTCCACCACTTCGCCAAGACTTCACCAACTCCACTGCAGATCTTGGACAACCCCCTACACCTGATTCTCCAAGCAGAAATATCCAACGGGGAAATCAAATATCCGAGTCATTGGCTTTAAAGAGTCGCTATGCTCCCTCCTCTAGTATCGCAGCCTCTGGTCTTTTTGTGCCTAAAACTACAGCTGATGCACTGGAAGAACTACAAAGCTATAGAGAGATGAAAAATTTGTTGATAAGGCAAGCTGGGAAAACTCACACATGAATCAAAATATGGTCTGAAGTAGGAGAAACCTTATTATAATCTCATGAAGTCAGTAGTACCATAATATGTGAATATCTTAAGTCCTATGAATACATGTACAGTAATTTCCTTTTCCAAGTTGCAGGTTCTGGAGCTTGAAACTACATTGGTTGGCTTCTATTTTTCTTCTTGTACATAATTTTATATAATTGGGGTGATCGAGCAGTTATGACCATATGACCCACCTCAGGGCACTTACACCTATAACCTCTGTATATAATATAAATGCAAACTTGCTAAATCTTTGAACTCTTTATTATCTTCTCATTGAAGCTAATTTGATCAGTTTATTTTAATAGAGATTAGATCTGTCTGTAGTGGTTTATGTAAG

Coding sequence (CDS)

ATGGCTTCTTCTCATAATACTCATTCCGAGACTGCAAAAGTTGAGCAAATTTTAACTGAATTCTTTCCTAAAACGCTACAAATTATACTGGAATCGAGGGCCCCTTGTATTTCATCGCGTAATTTTAGTGGCGAACAAGTTTTGTCTTCTCCATCTTCTTCCTCCTCGTCTTCCTCGAGTATGAGGCCGAGGGATAAATGGTTTAATTTGGCTCTTCGTGAATGCTCTGCAACGTTAGAGAATATTGATCTCTGGCGTCCGAATTATCATGAACCTATGGTTGTTGATGTTATTTTGGTGCAGAGGCAATTTGGTTTAGATTCTGTTAGTGCTTCTCCTAGGAAAGATCTTGTTAGGAATTTGTCTCTGAAAGAGAAATATCCACTCTCTTTCAATTCTGATAAGGATGAGTTTGGAAGTCAGACCAAGAGTGAGAAAGTTGTAGAGCGATGGATGGTGCATTATGAGAGTAGGAAAAACCGGGATAGTAATTCGGGTAGCAGAAGGTCCAGTAATAGTACTGCTCACACCTACAAGAAAACCATTTTACTCTTAAGGTCTTTATATGCCTTTGTGAGACTTTTACCTGCCTATAAGGTTTTTCAAGATATCAGTTCATCTGGTCAGATTCACCCTTTTACTCTTGCTCATCGTGTGTCTTCCTTTGCTGAGCCTTTCACCCGGAGGGAAGAGGCCGAAATGCAGCGATTTGTGTTCACTCCTGTCGACACTTCTTGTGGTCGACTATGCCTCTCAGTGTTATACCGTTCATCCCTCTCTGACATAAACTCTGAACCATCAACTCCTATGTCTCCTCAAGTTATTCCTGAATATGTTGGGAGTCCATTGGCAGATCCATTGAAAAGGTTTCCTACCCTCCCTGTAACGATAGCACCATCTCATGGATCTCCATCGTCGTTGCCATTCTCAAGACGTCATAGTTGGAGTTATGACCGTTTTAGACCTTCCCCTCCTTCAGTCTCCTTTTCACCTTCACCAACACATTCAGAGTCTCATGCTTTAATTTCAAATCCAGCCTTTCCCCGTTTACCTCCTTCAAGCTTACCTTCACAACTGCCTGAAATGGTTACGGGACATAAAGATAATATGAACTACGATGAATATTACCCTTCTCCTGTCTTTTCTCAATCACCCTCACTGTCACCACCAATTCGCATTCGTGTAAAGCGCCTGCCAAATGGTCTATTGCAGTCTGAAAGTGCTCCTCCAAGTGCACCTATTGCAAAGCTTCCTCATTCCCCTGCATTATCAAGCAAACCGAATTTGCCACCATCCCCTCCCCTTAAAGCTTCTGGAGCAATTATTTCTAGGATTAATAGAGATGTTGGTCCATTGCCGGCTGGCTCTGCAATTGGAAAGTCATCTTCTCTTGGACGAGATGAAAGCCAAAGAATTTCAGGCTGGAGGGTATCATCCAATAACTCACCAATTTCAAGAAGCTCTAGTAGATCTTTTCCAGATGACTTAGATGATCCAGAGTTTCCATGCCCCTTTGATGTGGATGAGGATGAGATGACAGATAGAGGTAGCAGGCCTGAATCCTTCGACCAAAAAGGAAATCTTTGCGACATGCTGGAACCCGGGGGATTTTTTCCGATCCGTAAATCTCAAGATGCTGCTGTTGGTGCTCTAGTACGTATGCTTCAAAAAGCTCCACCACTTCGCCAAGACTTCACCAACTCCACTGCAGATCTTGGACAACCCCCTACACCTGATTCTCCAAGCAGAAATATCCAACGGGGAAATCAAATATCCGAGTCATTGGCTTTAAAGAGTCGCTATGCTCCCTCCTCTAGTATCGCAGCCTCTGGTCTTTTTGTGCCTAAAACTACAGCTGATGCACTGGAAGAACTACAAAGCTATAGAGAGATGAAAAATTTGTTGATAAGGCAAGCTGGGAAAACTCACACATGA

Protein sequence

MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFTPVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPSHGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHSPALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSNNSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPIRKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSRYAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT
Homology
BLAST of Cucsat.G1706.T4 vs. ExPASy Swiss-Prot
Match: F4J8V5 (Autophagy-related protein 13b OS=Arabidopsis thaliana OX=3702 GN=ATG13B PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.3e-162
Identity = 353/647 (54.56%), Postives = 443/647 (68.47%), Query Frame = 0

Query: 1   MASSH----NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSS 60
           M+SSH    N +SE AK EQI+ EFF K+L IILESR P +SSRNFSGEQ++ SPSSSSS
Sbjct: 1   MSSSHNRSNNNNSEGAKAEQIIFEFFAKSLHIILESRTPFMSSRNFSGEQMICSPSSSSS 60

Query: 61  SSSSMRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKD 120
           SSSS+RPRDKWFNLALREC A LE+ D+ R +  EP+VVDV+LV R    D    S +++
Sbjct: 61  SSSSVRPRDKWFNLALRECPAALESFDIGRRSSLEPLVVDVVLVVRPLVGDQ---SGKRE 120

Query: 121 LVRNLSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS--NSGSRRSSNST 180
           L+RN S K+ Y   +NSD+DE G +TK+E+++ERW+V Y++RK R+S   S  R SSN  
Sbjct: 121 LIRNFSGKD-YQSGWNSDQDELGCETKNEQIIERWVVQYDNRKIRESVTTSSRRSSSNKL 180

Query: 181 AHTYKKTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEM 240
              YKK  LLLRSL+  VRLLPAYK+F++++SSGQI  F L  RV S  EPFTR+EEAEM
Sbjct: 181 QVMYKKATLLLRSLFVMVRLLPAYKIFRELNSSGQIFKFKLVPRVPSIVEPFTRKEEAEM 240

Query: 241 QRFVFTPVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLP 300
           Q+F FTPV+T CGRLCLSVLYR SLSD++ E STPMSP  I +YVGSPLADPLKRFP+LP
Sbjct: 241 QKFSFTPVETICGRLCLSVLYR-SLSDVSCEHSTPMSPTFITDYVGSPLADPLKRFPSLP 300

Query: 301 VTIAPSHGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSS 360
           +    S+GSP  LPF RRHSWS+DR++ SPPSVS SPSPT S+SHAL+S+P    LPP  
Sbjct: 301 L----SYGSPPLLPFQRRHSWSFDRYKASPPSVSCSPSPTRSDSHALVSHPCSRHLPPH- 360

Query: 361 LPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPI 420
            PS +P   TG +     +EY P   FS  PS S P       +P G+ ++ESAP   P 
Sbjct: 361 -PSDIP---TGRRKESYPEEYSPCQDFSPPPSPSAP----KHAVPRGITRTESAPVRIP- 420

Query: 421 AKLPHSPALSSKPN-LPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRIS 480
                +P   SK N + PS  LK S     +  R++GP  +G+AI K    GRD+ +R S
Sbjct: 421 -----APTFQSKENVVAPSAHLKLSRHASLKPVRNLGPGESGAAIDKLFLYGRDDFRRPS 480

Query: 481 GWRVSSNNSP---ISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCD 540
           G R SS++SP    SRSSSRSF DD DDP+FPCPFDV+ D++TDR SRP SFD +G++ +
Sbjct: 481 GVRPSSSSSPRISFSRSSSRSFQDDFDDPDFPCPFDVEYDDITDRNSRPGSFDHRGDIHE 540

Query: 541 MLEPGGFFPIRKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQ 600
             +  G +P +KSQDAAVGALVRML+KAPPLRQD + S+        P+  S N +    
Sbjct: 541 PFDSSGSYP-KKSQDAAVGALVRMLKKAPPLRQDVSESS-------RPEICSNNNKPAGA 600

Query: 601 ISESLALKSRYAPSSSIAASGL-FVPKTTADALEELQSYREMKNLLI 637
              ++A         SI ASG+    KTTADALEEL+SY+EMKN L+
Sbjct: 601 HEIAVA---------SITASGIALASKTTADALEELRSYKEMKNHLL 606

BLAST of Cucsat.G1706.T4 vs. ExPASy Swiss-Prot
Match: Q9SCK0 (Autophagy-related protein 13a OS=Arabidopsis thaliana OX=3702 GN=ATG13A PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.1e-59
Identity = 223/675 (33.04%), Postives = 320/675 (47.41%), Query Frame = 0

Query: 6   NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRD 65
           N  S+  ++EQI++ FFPK L I+L SR P + SR  + E++         S  ++R  D
Sbjct: 6   NLPSDIGRLEQIVSHFFPKALHIVLNSRIPSLQSRGRTRERL---------SGLNVRKSD 65

Query: 66  KWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKE 125
           KWFNL + +  A LE +  W  N  + M++D+ILV                         
Sbjct: 66  KWFNLVMGDRPAALEKLHSWHRNILDSMIIDIILV------------------------- 125

Query: 126 KYPLS---FNSDKDEFGSQTKS-EKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKT 185
            +P+S    + D D   S  +S E V+ERW+V YE+       S    S+      YKK+
Sbjct: 126 -HPISNDNLDDDDDHSDSVVRSAETVIERWVVQYENPLIMSPQSSD--SATRYQKVYKKS 185

Query: 186 ILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFTP 245
           I+LLRSLYA  RLLPAY+V + +SSS     + L ++VSSF++ F+      M+ F F P
Sbjct: 186 IILLRSLYAQTRLLPAYRVSRQLSSSLASSGYDLIYKVSSFSDIFSGPVTETMKEFRFAP 245

Query: 246 VDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPSH 305
           V+   GRLC SV YRS LSD N      + P++I +YVGSP  DP++ FP+ P      H
Sbjct: 246 VEVPPGRLCASVTYRSDLSDFNLGAHITLPPRIITDYVGSPATDPMRFFPS-PGRSVEGH 305

Query: 306 ------GSP--SSLPFSRRHSWSYDRFRP----SPPSVSFSPSPTHSESHALISNPAFPR 365
                 G P  +     R HSW+    RP    + P+ SFSP+ +H              
Sbjct: 306 SFTGRAGRPPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSH-------------- 365

Query: 366 LPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAP 425
                L   L +      D    D +  SP FS S S S P  I     P   ++  +AP
Sbjct: 366 ----QLSPGLHDFHWSRTDAFG-DNHQLSPPFSPSGSPSTPRYISGGNSPRINVRPGTAP 425

Query: 426 PSAPIAKLPHSPALS--SKPNLPPSPPLKASGAIISRINRDVGP---LPAGSAIGKSSS- 485
            + P +   +    S  S+P   P PP        S  ++D  P   L   S  G+S S 
Sbjct: 426 VTIPSSATLNRYVSSNFSEPGRNPLPPFSPKSTRRSPSSQDSLPGIALYRSSRSGESPSG 485

Query: 486 ---------LGRD---ESQRISGWRVSSNNSP---ISRSSSR-SFPDDLDDPEFPCPFDV 545
                    L +D   +S R SG  +SS++SP    SRS SR S  DDLDDP+  CPFD 
Sbjct: 486 LMNQYPTQKLSKDSKYDSGRFSG-VLSSSDSPRFAFSRSPSRLSSQDDLDDPDCSCPFDF 545

Query: 546 DE-DEMTDRGSRPESFDQKGNLCDMLEPGGFFPI--RKSQDAAVGALVRMLQKAPPLRQD 605
           D+ DE   + S   S D++     + +     P+  R SQDAAVG LV ML+ APPLRQD
Sbjct: 546 DDVDESGLQYS--HSLDRRKTSSSISQS---LPLGRRSSQDAAVGVLVHMLKTAPPLRQD 593

Query: 606 FTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSRYAPSSSIAASGLFVPKTTADALEE 640
            +   A +            +QR   +S +               S   + ++T+DALEE
Sbjct: 606 SSTYMASMS----------GVQREGSVSGT--------------ESEFSMARSTSDALEE 593

BLAST of Cucsat.G1706.T4 vs. ExPASy Swiss-Prot
Match: Q6C315 (Autophagy-related protein 13 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ATG13 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.6e-17
Identity = 96/342 (28.07%), Postives = 150/342 (43.86%), Query Frame = 0

Query: 3   SSHNTHSETAKVEQILTEFFPKTLQIILESRA-PCISSRNFSGEQVLSSPSSSSSSSSSM 62
           SS ++HS  +K+ Q++  FF K  Q+++++R  P +  +  SG++               
Sbjct: 7   SSGSSHSSKSKLNQVVQNFFSKATQVVVQARLNPRLREQRQSGDK--------------- 66

Query: 63  RPRDKWFNLALRECSATLENIDLWRP------NYHEPMVVDVILVQRQFGLDSVSASPRK 122
              +KWFNL   E  A  E++ LWR       +   P+VV+  L  R         SP +
Sbjct: 67  HKLNKWFNLETDELEAYREDLKLWRTIDIYNMDKMPPVVVETYLDMRHL-------SPNQ 126

Query: 123 DLVRNLSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSG----SRRSS 182
            LV   +  +++  SF           K+E V+ERW++  +     D  +G    S  SS
Sbjct: 127 TLVLEDAFGKRWNASFGG--------RKTEVVLERWVIEIQ---RPDPQAGGATTSPASS 186

Query: 183 NSTAHTYKKTILLLRSLYAFVRLLPAYKVFQDISSSG-QIHPFTLAHRVSSFAEPFTRR- 242
                 YKK ILL RSLY + RLLPA+ + + +S S     P  +  RV + ++P + R 
Sbjct: 187 TELPMAYKKCILLFRSLYTYCRLLPAWTLQKRLSKSKLSTSPLRIGCRVLNGSQPISSRG 246

Query: 243 ------------EEAEMQRFVFTPVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVI-- 302
                       E   +Q F F PV+   G   +SV YR + +    +    +S Q +  
Sbjct: 247 RVGLSKLIAGSSESQHLQTFSFDPVEIPSGVFKISVSYRVNCNFAVDDSEAMLSSQFLRI 306

Query: 303 -PEYVGSPLADPLKRFPTLPVTIAPSHGSPSSLPFSRRHSWS 317
             E    P + P+K     P   AP+    + LP  RRHS S
Sbjct: 307 DEEKPVVPPSPPVKH----PSMAAPNLHYITGLP--RRHSHS 309

BLAST of Cucsat.G1706.T4 vs. ExPASy Swiss-Prot
Match: Q5A1Z5 (Autophagy-related protein 13 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATG13 PE=3 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 5.5e-12
Identity = 116/451 (25.72%), Postives = 188/451 (41.69%), Query Frame = 0

Query: 6   NTHSET------AKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSS 65
           N+H +T      AK+ Q++ +FF K  QIILESRA              S+PS   +   
Sbjct: 20  NSHDDTQTKLQVAKLTQVIQKFFTKAAQIILESRA----------YPETSTPSLYPTKEE 79

Query: 66  SMRPRDKWFNLALREC-SATLENIDLWRP---NYHEPMVVDVILVQRQFGLDSVSASPRK 125
           S +  +KWFNL +     +  +++ LW+        PM+++  +  R    D        
Sbjct: 80  SSK-INKWFNLYMTNIPDSCKDDLKLWKGVDLTTIPPMIIETYIDLRSLPADQTLV---- 139

Query: 126 DLVRNLSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTA 185
                L   EK+P +    + +     K E V+ERW++ +E      + + +        
Sbjct: 140 -----LMDDEKHPWTVAKSRGK-----KQEVVLERWLIEFE-----PNTTDATVMVEELP 199

Query: 186 HTYKKTILLLRSLYAFVRLLPAYKVFQDISSSGQI--------HPFTLAHRVSSFAEPF- 245
            +YK+ I+L RS+Y F RL+PA+KV +++ +   +         P +   R+   ++P  
Sbjct: 200 LSYKQAIVLFRSIYGFTRLMPAFKVKKNLQNKLPLGNKILDGNQPISSKGRI-GLSKPII 259

Query: 246 -TRREEAEMQRFVFTPVDTSCGRLCLSVLYR----------------------------- 305
            TR  E+ M +  F PV TS G L +SV YR                             
Sbjct: 260 NTRTNESHMTQKYFQPVHTSLGTLKISVAYRMDSEFCLHENEELLSSHFHKRDEEETKKK 319

Query: 306 --SSLSDINSEPS---TPMSP-------QVIPEYVGSPLADPLKRFPTL--PVTIAPSHG 365
             SS+S ++S  S   T  SP       ++ P  VGS    P  + P++  P T AP   
Sbjct: 320 VSSSVSPLSSGTSLKETSTSPRKSQPPIRIQPFKVGSMSTSPPVQSPSISQPGT-APIQN 379

Query: 366 SPS--SLPFSRRHSWSYDRFRPSPPSVSF-----SPSPTHSESHALISNPAFPRLPPSSL 385
            PS  S    RR S + ++   +    +F     S +P+ +    + +NP      P S 
Sbjct: 380 QPSVPSSSLERRVSITSNKSTSNASLAAFLRNARSSTPSANNIPIINANPISGTSVPRSF 433

BLAST of Cucsat.G1706.T4 vs. ExPASy Swiss-Prot
Match: Q6BQ20 (Autophagy-related protein 13 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=ATG13 PE=3 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 2.1e-11
Identity = 98/418 (23.44%), Postives = 171/418 (40.91%), Query Frame = 0

Query: 10  ETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRDKWFN 69
           + AK+ Q++  FF K++QI+L+SR   I S + + E+ L         + S +  +KWFN
Sbjct: 23  QNAKLTQVIQNFFSKSVQIVLQSR---IQSESHNKEEQLKVGGDVGGHNVSSK-INKWFN 82

Query: 70  LALRECSATLENIDLWR----PNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKE 129
           L +   +   E + LW+     +   PM+++V L  RQ              ++ + L++
Sbjct: 83  LHMYNDNLPKEELKLWKNINDVSQMPPMIIEVYLDLRQL-----------TAIQTVILRD 142

Query: 130 KYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKTILLL 189
                +   K   G   K E V+ERW++ +      D+N+ S    +     YK+ I+L 
Sbjct: 143 DNGNPWTVAK---GGSKKHEVVLERWLIEF------DANTVSGTIVDELPLIYKQAIILF 202

Query: 190 RSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRR---------------- 249
           RSLY + RL+P +K+ ++++ S       +  ++    +P + +                
Sbjct: 203 RSLYGYTRLMPTFKLKKNLNKSN----LNIGCKILDGKQPISSKGRIGLSKSIIPHQMLT 262

Query: 250 EEAEMQRFVFTPVDTSCGRLCLSVLYRS----SLSDINSEPST--------PMSPQVIPE 309
            E+ M    F P+ T+ G L +S+ YR+    S+ D     ST            ++ P 
Sbjct: 263 TESHMSHKHFLPIQTTLGTLKISIAYRNHHEFSIHDNEELLSTHFVNIDDNDKDSEITPV 322

Query: 310 YVGSPLADPLKRFPTLPVTIAPSHGSPS---SLPFSRRHSWSYDRFRPSPPSVSFSPSPT 369
            +       + R  T    +       S    +  S+ H  S +      P  SF     
Sbjct: 323 EIEFKEELKIDRDSTTNEKVNEPEDDESHHADVESSQEHLQSEE------PDESFEQDAK 382

Query: 370 H---SESHALISNPAFPRLPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSP 390
           H   S     ISN A   L P    S  P+ VT    + N      +P+ SQ P+++P
Sbjct: 383 HISGSRKKFSISNNASMSLSPC---SSGPQTVTEDSPSHNKPSANTTPIVSQRPTINP 403

BLAST of Cucsat.G1706.T4 vs. NCBI nr
Match: XP_004148643.1 (autophagy-related protein 13b [Cucumis sativus] >KAE8647466.1 hypothetical protein Csa_003325 [Cucumis sativus])

HSP 1 Score: 1246 bits (3223), Expect = 0.0
Identity = 644/644 (100.00%), Postives = 644/644 (100.00%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS
Sbjct: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP
Sbjct: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS
Sbjct: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN
Sbjct: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644
           YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT
Sbjct: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644

BLAST of Cucsat.G1706.T4 vs. NCBI nr
Match: XP_008441017.1 (PREDICTED: autophagy-related protein 13b isoform X1 [Cucumis melo])

HSP 1 Score: 1226 bits (3173), Expect = 0.0
Identity = 634/644 (98.45%), Postives = 639/644 (99.22%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSV+ASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS
Sbjct: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP
Sbjct: 301 HGSPSSLSFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EMV GHKDNMNYDEYYPSPVFSQSPSLSPPIRI VKRLPNGLLQSESAPPSAPIAKLPHS
Sbjct: 361 EMVIGHKDNMNYDEYYPSPVFSQSPSLSPPIRIPVKRLPNGLLQSESAPPSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFP+
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPL 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQ GNQISESLA KSR
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQLGNQISESLASKSR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644
           YAPSSSIAASGLFVPKTTADALEELQSYREMKNLL+RQAGKTHT
Sbjct: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLLRQAGKTHT 644

BLAST of Cucsat.G1706.T4 vs. NCBI nr
Match: XP_038882060.1 (autophagy-related protein 13b [Benincasa hispida])

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 608/644 (94.41%), Postives = 623/644 (96.74%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRP+YHEPMVVDVILVQRQFGLDSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVVDVILVQRQFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
            SLKEKYPLSFNSDKDEFG QTKSEKV+ERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK
Sbjct: 121 SSLKEKYPLSFNSDKDEFGCQTKSEKVIERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSS QIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSCQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYRSSLSDINSEPSTP+SPQVIPEYVGSPLADPLKRFPTLPVTIAP+
Sbjct: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPISPQVIPEYVGSPLADPLKRFPTLPVTIAPT 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAF RLPPSSLPS  P
Sbjct: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFCRLPPSSLPSHPP 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EMV GHKDNMNYDEYYPS VFSQSPSLSPPI I V+RLPNGLLQSESAPPSAPIAKLPHS
Sbjct: 361 EMVMGHKDNMNYDEYYPSSVFSQSPSLSPPIGILVRRLPNGLLQSESAPPSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNLPPSPPLKASG IISRINRD+GPL AGSAIGKSSSLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLPPSPPLKASGPIISRINRDIGPLLAGSAIGKSSSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVD+DEM DRGSRPESFDQKGNLC+MLEPGGFFP 
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDDDEMIDRGSRPESFDQKGNLCEMLEPGGFFPS 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKA PLRQDFTN TADLG+PPTPDSPSRNIQ+GNQISESLA KSR
Sbjct: 541 RKSQDAAVGALVRMLQKASPLRQDFTNFTADLGRPPTPDSPSRNIQQGNQISESLASKSR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644
           YA +SS+ ASGLFVPKTTADALEELQSYREMKNLL+RQAG T T
Sbjct: 601 YA-ASSVTASGLFVPKTTADALEELQSYREMKNLLLRQAGNTRT 643

BLAST of Cucsat.G1706.T4 vs. NCBI nr
Match: KAG7034429.1 (Autophagy-related protein 13b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1102 bits (2849), Expect = 0.0
Identity = 574/642 (89.41%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESR+PCISSRNFSG+QVL SPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRSPCISSRNFSGDQVLCSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRP+YHEPMV+DVILVQR+FGLDSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVIDVILVQREFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLK+K+P S NSDK+EFG QTKSEKVVERWMVHYESRKNRD+NSG RRSSNSTAHTYKK
Sbjct: 121 LSLKDKHPFSLNSDKEEFGCQTKSEKVVERWMVHYESRKNRDTNSGCRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTL HRVSSF EPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLVHRVSSFVEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYR+SLSDI+SEPSTPMSPQVIPEYVGSPL DPLKRFPTLPV+IAPS
Sbjct: 241 PVDTSCGRLCLSVLYRASLSDISSEPSTPMSPQVIPEYVGSPLTDPLKRFPTLPVSIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYDRFRPSPPSVSFSPSPT+SESHAL+SNPAF RLPPSSLPSQ  
Sbjct: 301 HGSPSSLQFSRRHSWSYDRFRPSPPSVSFSPSPTYSESHALVSNPAFCRLPPSSLPSQPA 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EM  GHKDN+NYDEYYPSPVFS SPSLSPPI I V+RLPNGLLQSESAP SAPIAKLPHS
Sbjct: 361 EMAMGHKDNVNYDEYYPSPVFSPSPSLSPPIGIPVRRLPNGLLQSESAPQSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNL PS PLKASG  I        PLPAGSAI KS SLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLSPSSPLKASGPGI--------PLPAGSAIRKSFSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGN+C+MLEPGGFFPI
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNICEMLEPGGFFPI 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFT+S ADL +P TPDSPSRN Q+G QIS+SL  K R
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTDSAADLVRPSTPDSPSRNPQQGTQISKSLGSKGR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKT 642
           YA SSSI  SGLFVPKTTADALEELQSYREMKNLL+RQAGKT
Sbjct: 601 YAASSSITTSGLFVPKTTADALEELQSYREMKNLLLRQAGKT 634

BLAST of Cucsat.G1706.T4 vs. NCBI nr
Match: KAG6604273.1 (Autophagy-related protein 13b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 573/642 (89.25%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESR+PCISSRNFSG+QVL SPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRSPCISSRNFSGDQVLCSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRP+YHEPMV+DVILVQR+FGLDSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVIDVILVQREFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLK+K+P S NSDK+EFG QTKSEKVVERWMVHYESRKNRD+NSG RRSSNSTAHTYKK
Sbjct: 121 LSLKDKHPFSLNSDKEEFGCQTKSEKVVERWMVHYESRKNRDTNSGCRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTL HRVSSF EPFTR+EEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLVHRVSSFVEPFTRKEEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYR+SLSDI+SEPSTPMSPQVIPEYVGSPL DPLKRFPTLPV+IAPS
Sbjct: 241 PVDTSCGRLCLSVLYRASLSDISSEPSTPMSPQVIPEYVGSPLTDPLKRFPTLPVSIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYDRFRPSPPSVSFSPSPT+SESHAL+SNPAF RLPPSSLPSQ  
Sbjct: 301 HGSPSSLQFSRRHSWSYDRFRPSPPSVSFSPSPTYSESHALVSNPAFCRLPPSSLPSQPA 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EM  GHKDN+NYDEYYPSPVFS SPSLSPPI I V+RLPNGLLQSESAP SAPIAKLPHS
Sbjct: 361 EMAMGHKDNVNYDEYYPSPVFSPSPSLSPPIGIPVRRLPNGLLQSESAPQSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNL PS PLKASG  I        PLPAGSAI KS SLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLSPSSPLKASGPGI--------PLPAGSAIRKSFSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGN+C+MLEPGGFFPI
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNICEMLEPGGFFPI 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFT+S ADL +P TPDSPSRN Q+G QIS+SL  K R
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTDSAADLVRPSTPDSPSRNPQQGTQISKSLGSKGR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKT 642
           YA SSSI  SGLFVPKTTADALEELQSYREMKNLL+RQAGKT
Sbjct: 601 YAASSSITTSGLFVPKTTADALEELQSYREMKNLLLRQAGKT 634

BLAST of Cucsat.G1706.T4 vs. ExPASy TrEMBL
Match: A0A1S3B2G3 (autophagy-related protein 13b isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485257 PE=4 SV=1)

HSP 1 Score: 1226 bits (3173), Expect = 0.0
Identity = 634/644 (98.45%), Postives = 639/644 (99.22%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSV+ASPRKDLVR+
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVNASPRKDLVRH 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS
Sbjct: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP
Sbjct: 301 HGSPSSLSFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EMV GHKDNMNYDEYYPSPVFSQSPSLSPPIRI VKRLPNGLLQSESAPPSAPIAKLPHS
Sbjct: 361 EMVIGHKDNMNYDEYYPSPVFSQSPSLSPPIRIPVKRLPNGLLQSESAPPSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFP+
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPL 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQ GNQISESLA KSR
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQLGNQISESLASKSR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644
           YAPSSSIAASGLFVPKTTADALEELQSYREMKNLL+RQAGKTHT
Sbjct: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLLRQAGKTHT 644

BLAST of Cucsat.G1706.T4 vs. ExPASy TrEMBL
Match: A0A6J1IKL7 (autophagy-related protein 13b OS=Cucurbita maxima OX=3661 GN=LOC111477911 PE=4 SV=1)

HSP 1 Score: 1099 bits (2842), Expect = 0.0
Identity = 573/642 (89.25%), Postives = 599/642 (93.30%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESR+PCISSRNFSG+QVL SPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRSPCISSRNFSGDQVLCSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRP+YHEPMV+DVILVQR+FGLDSV+ASPR+DLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVIDVILVQREFGLDSVNASPRRDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLKEK+P S NSDK+EFG QTKSEKVVERWMVHYESRKNRD+NSG RRSSNSTAHTYKK
Sbjct: 121 LSLKEKHPFSLNSDKEEFGCQTKSEKVVERWMVHYESRKNRDTNSGCRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTL HRVSSF EPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLVHRVSSFVEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYR+SLSDI+SEPSTPMSPQVIPEYVGSPL DPLKRFPTLPV+IAPS
Sbjct: 241 PVDTSCGRLCLSVLYRASLSDISSEPSTPMSPQVIPEYVGSPLTDPLKRFPTLPVSIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYDRFRPSPPSVSFSPSPT+SESHAL+SNPAF RLPPSSLPSQ  
Sbjct: 301 HGSPSSLQFSRRHSWSYDRFRPSPPSVSFSPSPTYSESHALVSNPAFCRLPPSSLPSQPA 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EM  GHKDN+NYDEYYPSPVFS SPSLSPPI I V+RLPNGLLQSESAP SAPIAKLPHS
Sbjct: 361 EMAMGHKDNVNYDEYYPSPVFSPSPSLSPPIGIPVRRLPNGLLQSESAPQSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNL PS PLKASG  I        PLPAGSAI KS SLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLSPSSPLKASGPGI--------PLPAGSAIRKSFSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGN+C+MLE GGFFPI
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNICEMLETGGFFPI 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFT+S ADL +P TPDSPSRN Q+G QIS+SLA K R
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTDSAADLVRPSTPDSPSRNPQQGTQISKSLASKGR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKT 642
           YA SSSI  SGLFVPKTTADALEELQSYREMKNLL++QAGKT
Sbjct: 601 YAASSSITTSGLFVPKTTADALEELQSYREMKNLLLKQAGKT 634

BLAST of Cucsat.G1706.T4 vs. ExPASy TrEMBL
Match: A0A6J1GDD7 (autophagy-related protein 13b OS=Cucurbita moschata OX=3662 GN=LOC111452961 PE=4 SV=1)

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 571/642 (88.94%), Postives = 596/642 (92.83%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESR+PCISSRNFSG+QVL SPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRSPCISSRNFSGDQVLCSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRP+YHEPMV+DVILVQR+FGLDSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPSYHEPMVIDVILVQREFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLK+K+P S NSDK+EFG QTKSEKVVERWMVHYESRKNRD+NSG RRSSNSTAHTYKK
Sbjct: 121 LSLKDKHPFSLNSDKEEFGCQTKSEKVVERWMVHYESRKNRDTNSGCRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTL HRVSSF EPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLVHRVSSFVEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYR+SLSDI+SEPSTPMSPQVIPEYVGSPL DPLKRFPTLPV+IAPS
Sbjct: 241 PVDTSCGRLCLSVLYRASLSDISSEPSTPMSPQVIPEYVGSPLTDPLKRFPTLPVSIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSL FSRRHSWSYD FRPSPPSVSFSPSPT+SESHAL+SNPAF RLPPSSLP Q  
Sbjct: 301 HGSPSSLQFSRRHSWSYDHFRPSPPSVSFSPSPTYSESHALVSNPAFCRLPPSSLPPQPA 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EM  GHKDN+NYDEYYPSPVFS SPSLSPPI I V+RLPNGLLQSESAP SAPIAKLPHS
Sbjct: 361 EMAMGHKDNVNYDEYYPSPVFSPSPSLSPPIGIPVRRLPNGLLQSESAPQSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNL PS PLKASG  I        PLPAGSAI KS SLGRDES+RISGWRVSSN
Sbjct: 421 PALSSKPNLSPSSPLKASGPGI--------PLPAGSAIRKSFSLGRDESRRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGGFFPI 540
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGN+C+MLEPGGFFPI
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNICEMLEPGGFFPI 540

Query: 541 RKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSR 600
           RKSQDAAVGALVRMLQKAPPLRQDFT+  ADL +P TPDSPSRN Q+G QIS+SL  K R
Sbjct: 541 RKSQDAAVGALVRMLQKAPPLRQDFTDPAADLVRPSTPDSPSRNPQQGTQISKSLGSKGR 600

Query: 601 YAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKT 642
           YA SSSI  SGLFVPKTTADALEELQSYREMKNLL+RQAGKT
Sbjct: 601 YAASSSITTSGLFVPKTTADALEELQSYREMKNLLLRQAGKT 634

BLAST of Cucsat.G1706.T4 vs. ExPASy TrEMBL
Match: A0A6J1BUH2 (autophagy-related protein 13b OS=Momordica charantia OX=3673 GN=LOC111005657 PE=4 SV=1)

HSP 1 Score: 1094 bits (2829), Expect = 0.0
Identity = 569/648 (87.81%), Postives = 600/648 (92.59%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAK EQILTEFF KTLQI+LESR+PCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKTEQILTEFFAKTLQIVLESRSPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSA LENIDLWR +YHEPMVVDVILVQRQFGLDSV+ASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSAALENIDLWRQSYHEPMVVDVILVQRQFGLDSVNASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHT-YK 180
           LSLK+KY LSFNSDKDEFG  TKSEKVVERWMVHYE+RKNRD NSGSRRS  +T HT YK
Sbjct: 121 LSLKDKYSLSFNSDKDEFGCDTKSEKVVERWMVHYETRKNRDGNSGSRRSGTNTLHTFYK 180

Query: 181 KTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVF 240
           KTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRF F
Sbjct: 181 KTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFAF 240

Query: 241 TPVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAP 300
           TPVDTSCGRLCLSVLYRSSLSDI+SEPSTPMSPQVIPEYVGSPLADPLKRFP+LPVTIAP
Sbjct: 241 TPVDTSCGRLCLSVLYRSSLSDISSEPSTPMSPQVIPEYVGSPLADPLKRFPSLPVTIAP 300

Query: 301 SHGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQL 360
           S GS SSLPFSRRHSWSYDR+R SPPSVSFSPSPTHSESHALISNPAF RLPPSSLP   
Sbjct: 301 SQGSTSSLPFSRRHSWSYDRYRASPPSVSFSPSPTHSESHALISNPAFRRLPPSSLPPHP 360

Query: 361 PEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPH 420
           PEMV GHK+NMNYDEYYPSPV+S SPSLSPPI I V+RLPNGLLQSESAPPSAPIAKLPH
Sbjct: 361 PEMVLGHKENMNYDEYYPSPVYSNSPSLSPPIGIPVRRLPNGLLQSESAPPSAPIAKLPH 420

Query: 421 SPALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSS 480
           SPAL SKPNLPPSPPLKASG  IS+INRD+GPL  GSAIGKSSSLGRDE++R+SGWR SS
Sbjct: 421 SPALLSKPNLPPSPPLKASGPGISKINRDIGPLQGGSAIGKSSSLGRDETRRLSGWRSSS 480

Query: 481 NNSP---ISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCDMLEPGG 540
           NNSP   ISRSSSRSFPDDLDD EFPCPFDVD+DE+TDRGSRPESFD KGNLC+MLEPGG
Sbjct: 481 NNSPQISISRSSSRSFPDDLDDTEFPCPFDVDDDELTDRGSRPESFDPKGNLCEMLEPGG 540

Query: 541 FFPIRKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLA 600
           FFPIRKSQDAA+GALV MLQKAPPLRQDFTNS  D  +  TPD PSRNIQ+GNQISE+++
Sbjct: 541 FFPIRKSQDAAIGALVCMLQKAPPLRQDFTNSATDHLRSSTPDFPSRNIQQGNQISETVS 600

Query: 601 LKSRYAPSSSIAASGLFVPKTTADALEELQSYREMKNLLIRQAGKTHT 644
            K RYA SSS+ ASGL +PKTTADALEELQ+YREMKNLL+RQAGKTHT
Sbjct: 601 TKDRYAASSSVTASGLIMPKTTADALEELQNYREMKNLLLRQAGKTHT 648

BLAST of Cucsat.G1706.T4 vs. ExPASy TrEMBL
Match: A0A0A0KFR2 (ATG13 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484560 PE=4 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60
           MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS
Sbjct: 1   MASSHNTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSS 60

Query: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120
           MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN
Sbjct: 61  MRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRN 120

Query: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180
           LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK
Sbjct: 121 LSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKK 180

Query: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240
           TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT
Sbjct: 181 TILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFT 240

Query: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300
           PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS
Sbjct: 241 PVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPS 300

Query: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360
           HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP
Sbjct: 301 HGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSSLPSQLP 360

Query: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420
           EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS
Sbjct: 361 EMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPIAKLPHS 420

Query: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480
           PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN
Sbjct: 421 PALSSKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRISGWRVSSN 480

Query: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSR 518
           NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSR
Sbjct: 481 NSPISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSR 518

BLAST of Cucsat.G1706.T4 vs. TAIR 10
Match: AT3G18770.1 (Autophagy-related protein 13 )

HSP 1 Score: 574.7 bits (1480), Expect = 9.3e-164
Identity = 353/647 (54.56%), Postives = 443/647 (68.47%), Query Frame = 0

Query: 1   MASSH----NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSS 60
           M+SSH    N +SE AK EQI+ EFF K+L IILESR P +SSRNFSGEQ++ SPSSSSS
Sbjct: 1   MSSSHNRSNNNNSEGAKAEQIIFEFFAKSLHIILESRTPFMSSRNFSGEQMICSPSSSSS 60

Query: 61  SSSSMRPRDKWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKD 120
           SSSS+RPRDKWFNLALREC A LE+ D+ R +  EP+VVDV+LV R    D    S +++
Sbjct: 61  SSSSVRPRDKWFNLALRECPAALESFDIGRRSSLEPLVVDVVLVVRPLVGDQ---SGKRE 120

Query: 121 LVRNLSLKEKYPLSFNSDKDEFGSQTKSEKVVERWMVHYESRKNRDS--NSGSRRSSNST 180
           L+RN S K+ Y   +NSD+DE G +TK+E+++ERW+V Y++RK R+S   S  R SSN  
Sbjct: 121 LIRNFSGKD-YQSGWNSDQDELGCETKNEQIIERWVVQYDNRKIRESVTTSSRRSSSNKL 180

Query: 181 AHTYKKTILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEM 240
              YKK  LLLRSL+  VRLLPAYK+F++++SSGQI  F L  RV S  EPFTR+EEAEM
Sbjct: 181 QVMYKKATLLLRSLFVMVRLLPAYKIFRELNSSGQIFKFKLVPRVPSIVEPFTRKEEAEM 240

Query: 241 QRFVFTPVDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLP 300
           Q+F FTPV+T CGRLCLSVLYR SLSD++ E STPMSP  I +YVGSPLADPLKRFP+LP
Sbjct: 241 QKFSFTPVETICGRLCLSVLYR-SLSDVSCEHSTPMSPTFITDYVGSPLADPLKRFPSLP 300

Query: 301 VTIAPSHGSPSSLPFSRRHSWSYDRFRPSPPSVSFSPSPTHSESHALISNPAFPRLPPSS 360
           +    S+GSP  LPF RRHSWS+DR++ SPPSVS SPSPT S+SHAL+S+P    LPP  
Sbjct: 301 L----SYGSPPLLPFQRRHSWSFDRYKASPPSVSCSPSPTRSDSHALVSHPCSRHLPPH- 360

Query: 361 LPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAPPSAPI 420
            PS +P   TG +     +EY P   FS  PS S P       +P G+ ++ESAP   P 
Sbjct: 361 -PSDIP---TGRRKESYPEEYSPCQDFSPPPSPSAP----KHAVPRGITRTESAPVRIP- 420

Query: 421 AKLPHSPALSSKPN-LPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRDESQRIS 480
                +P   SK N + PS  LK S     +  R++GP  +G+AI K    GRD+ +R S
Sbjct: 421 -----APTFQSKENVVAPSAHLKLSRHASLKPVRNLGPGESGAAIDKLFLYGRDDFRRPS 480

Query: 481 GWRVSSNNSP---ISRSSSRSFPDDLDDPEFPCPFDVDEDEMTDRGSRPESFDQKGNLCD 540
           G R SS++SP    SRSSSRSF DD DDP+FPCPFDV+ D++TDR SRP SFD +G++ +
Sbjct: 481 GVRPSSSSSPRISFSRSSSRSFQDDFDDPDFPCPFDVEYDDITDRNSRPGSFDHRGDIHE 540

Query: 541 MLEPGGFFPIRKSQDAAVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQ 600
             +  G +P +KSQDAAVGALVRML+KAPPLRQD + S+        P+  S N +    
Sbjct: 541 PFDSSGSYP-KKSQDAAVGALVRMLKKAPPLRQDVSESS-------RPEICSNNNKPAGA 600

Query: 601 ISESLALKSRYAPSSSIAASGL-FVPKTTADALEELQSYREMKNLLI 637
              ++A         SI ASG+    KTTADALEEL+SY+EMKN L+
Sbjct: 601 HEIAVA---------SITASGIALASKTTADALEELRSYKEMKNHLL 606

BLAST of Cucsat.G1706.T4 vs. TAIR 10
Match: AT3G49590.2 (Autophagy-related protein 13 )

HSP 1 Score: 231.9 bits (590), Expect = 1.5e-60
Identity = 223/675 (33.04%), Postives = 320/675 (47.41%), Query Frame = 0

Query: 6   NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRD 65
           N  S+  ++EQI++ FFPK L I+L SR P + SR  + E++         S  ++R  D
Sbjct: 6   NLPSDIGRLEQIVSHFFPKALHIVLNSRIPSLQSRGRTRERL---------SGLNVRKSD 65

Query: 66  KWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKE 125
           KWFNL + +  A LE +  W  N  + M++D+ILV                         
Sbjct: 66  KWFNLVMGDRPAALEKLHSWHRNILDSMIIDIILV------------------------- 125

Query: 126 KYPLS---FNSDKDEFGSQTKS-EKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKT 185
            +P+S    + D D   S  +S E V+ERW+V YE+       S    S+      YKK+
Sbjct: 126 -HPISNDNLDDDDDHSDSVVRSAETVIERWVVQYENPLIMSPQSSD--SATRYQKVYKKS 185

Query: 186 ILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFTP 245
           I+LLRSLYA  RLLPAY+V + +SSS     + L ++VSSF++ F+      M+ F F P
Sbjct: 186 IILLRSLYAQTRLLPAYRVSRQLSSSLASSGYDLIYKVSSFSDIFSGPVTETMKEFRFAP 245

Query: 246 VDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPSH 305
           V+   GRLC SV YRS LSD N      + P++I +YVGSP  DP++ FP+ P      H
Sbjct: 246 VEVPPGRLCASVTYRSDLSDFNLGAHITLPPRIITDYVGSPATDPMRFFPS-PGRSVEGH 305

Query: 306 ------GSP--SSLPFSRRHSWSYDRFRP----SPPSVSFSPSPTHSESHALISNPAFPR 365
                 G P  +     R HSW+    RP    + P+ SFSP+ +H              
Sbjct: 306 SFTGRAGRPPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSH-------------- 365

Query: 366 LPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAP 425
                L   L +      D    D +  SP FS S S S P  I     P   ++  +AP
Sbjct: 366 ----QLSPGLHDFHWSRTDAFG-DNHQLSPPFSPSGSPSTPRYISGGNSPRINVRPGTAP 425

Query: 426 PSAPIAKLPHSPALS--SKPNLPPSPPLKASGAIISRINRDVGP---LPAGSAIGKSSS- 485
            + P +   +    S  S+P   P PP        S  ++D  P   L   S  G+S S 
Sbjct: 426 VTIPSSATLNRYVSSNFSEPGRNPLPPFSPKSTRRSPSSQDSLPGIALYRSSRSGESPSG 485

Query: 486 ---------LGRD---ESQRISGWRVSSNNSP---ISRSSSR-SFPDDLDDPEFPCPFDV 545
                    L +D   +S R SG  +SS++SP    SRS SR S  DDLDDP+  CPFD 
Sbjct: 486 LMNQYPTQKLSKDSKYDSGRFSG-VLSSSDSPRFAFSRSPSRLSSQDDLDDPDCSCPFDF 545

Query: 546 DE-DEMTDRGSRPESFDQKGNLCDMLEPGGFFPI--RKSQDAAVGALVRMLQKAPPLRQD 605
           D+ DE   + S   S D++     + +     P+  R SQDAAVG LV ML+ APPLRQD
Sbjct: 546 DDVDESGLQYS--HSLDRRKTSSSISQS---LPLGRRSSQDAAVGVLVHMLKTAPPLRQD 593

Query: 606 FTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSRYAPSSSIAASGLFVPKTTADALEE 640
            +   A +            +QR   +S +               S   + ++T+DALEE
Sbjct: 606 SSTYMASMS----------GVQREGSVSGT--------------ESEFSMARSTSDALEE 593

BLAST of Cucsat.G1706.T4 vs. TAIR 10
Match: AT3G49590.1 (Autophagy-related protein 13 )

HSP 1 Score: 231.9 bits (590), Expect = 1.5e-60
Identity = 223/675 (33.04%), Postives = 320/675 (47.41%), Query Frame = 0

Query: 6   NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRD 65
           N  S+  ++EQI++ FFPK L I+L SR P + SR  + E++         S  ++R  D
Sbjct: 6   NLPSDIGRLEQIVSHFFPKALHIVLNSRIPSLQSRGRTRERL---------SGLNVRKSD 65

Query: 66  KWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKE 125
           KWFNL + +  A LE +  W  N  + M++D+ILV                         
Sbjct: 66  KWFNLVMGDRPAALEKLHSWHRNILDSMIIDIILV------------------------- 125

Query: 126 KYPLS---FNSDKDEFGSQTKS-EKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKT 185
            +P+S    + D D   S  +S E V+ERW+V YE+       S    S+      YKK+
Sbjct: 126 -HPISNDNLDDDDDHSDSVVRSAETVIERWVVQYENPLIMSPQSSD--SATRYQKVYKKS 185

Query: 186 ILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFTP 245
           I+LLRSLYA  RLLPAY+V + +SSS     + L ++VSSF++ F+      M+ F F P
Sbjct: 186 IILLRSLYAQTRLLPAYRVSRQLSSSLASSGYDLIYKVSSFSDIFSGPVTETMKEFRFAP 245

Query: 246 VDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPSH 305
           V+   GRLC SV YRS LSD N      + P++I +YVGSP  DP++ FP+ P      H
Sbjct: 246 VEVPPGRLCASVTYRSDLSDFNLGAHITLPPRIITDYVGSPATDPMRFFPS-PGRSVEGH 305

Query: 306 ------GSP--SSLPFSRRHSWSYDRFRP----SPPSVSFSPSPTHSESHALISNPAFPR 365
                 G P  +     R HSW+    RP    + P+ SFSP+ +H              
Sbjct: 306 SFTGRAGRPPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSH-------------- 365

Query: 366 LPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAP 425
                L   L +      D    D +  SP FS S S S P  I     P   ++  +AP
Sbjct: 366 ----QLSPGLHDFHWSRTDAFG-DNHQLSPPFSPSGSPSTPRYISGGNSPRINVRPGTAP 425

Query: 426 PSAPIAKLPHSPALS--SKPNLPPSPPLKASGAIISRINRDVGP---LPAGSAIGKSSS- 485
            + P +   +    S  S+P   P PP        S  ++D  P   L   S  G+S S 
Sbjct: 426 VTIPSSATLNRYVSSNFSEPGRNPLPPFSPKSTRRSPSSQDSLPGIALYRSSRSGESPSG 485

Query: 486 ---------LGRD---ESQRISGWRVSSNNSP---ISRSSSR-SFPDDLDDPEFPCPFDV 545
                    L +D   +S R SG  +SS++SP    SRS SR S  DDLDDP+  CPFD 
Sbjct: 486 LMNQYPTQKLSKDSKYDSGRFSG-VLSSSDSPRFAFSRSPSRLSSQDDLDDPDCSCPFDF 545

Query: 546 DE-DEMTDRGSRPESFDQKGNLCDMLEPGGFFPI--RKSQDAAVGALVRMLQKAPPLRQD 605
           D+ DE   + S   S D++     + +     P+  R SQDAAVG LV ML+ APPLRQD
Sbjct: 546 DDVDESGLQYS--HSLDRRKTSSSISQS---LPLGRRSSQDAAVGVLVHMLKTAPPLRQD 593

Query: 606 FTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSRYAPSSSIAASGLFVPKTTADALEE 640
            +   A +            +QR   +S +               S   + ++T+DALEE
Sbjct: 606 SSTYMASMS----------GVQREGSVSGT--------------ESEFSMARSTSDALEE 593

BLAST of Cucsat.G1706.T4 vs. TAIR 10
Match: AT3G49590.3 (Autophagy-related protein 13 )

HSP 1 Score: 225.3 bits (573), Expect = 1.4e-58
Identity = 221/693 (31.89%), Postives = 318/693 (45.89%), Query Frame = 0

Query: 6   NTHSETAKVEQILTEFFPKTLQIILESRAPCISSRNFSGEQVLSSPSSSSSSSSSMRPRD 65
           N  S+  ++EQI++ FFPK L I+L SR P + SR  + E++         S  ++R  D
Sbjct: 6   NLPSDIGRLEQIVSHFFPKALHIVLNSRIPSLQSRGRTRERL---------SGLNVRKSD 65

Query: 66  KWFNLALRECSATLENIDLWRPNYHEPMVVDVILVQRQFGLDSVSASPRKDLVRNLSLKE 125
           KWFNL + +  A LE +  W  N  + M++D+ILV                         
Sbjct: 66  KWFNLVMGDRPAALEKLHSWHRNILDSMIIDIILV------------------------- 125

Query: 126 KYPLS---FNSDKDEFGSQTKS-EKVVERWMVHYESRKNRDSNSGSRRSSNSTAHTYKKT 185
            +P+S    + D D   S  +S E V+ERW+V YE+       S    S+      YKK+
Sbjct: 126 -HPISNDNLDDDDDHSDSVVRSAETVIERWVVQYENPLIMSPQSSD--SATRYQKVYKKS 185

Query: 186 ILLLRSLYAFVRLLPAYKVFQDISSSGQIHPFTLAHRVSSFAEPFTRREEAEMQRFVFTP 245
           I+LLRSLYA  RLLPAY+V + +SSS     + L ++VSSF++ F+      M+ F F P
Sbjct: 186 IILLRSLYAQTRLLPAYRVSRQLSSSLASSGYDLIYKVSSFSDIFSGPVTETMKEFRFAP 245

Query: 246 VDTSCGRLCLSVLYRSSLSDINSEPSTPMSPQVIPEYVGSPLADPLKRFPTLPVTIAPSH 305
           V+   GRLC SV YRS LSD N      + P++I +YVGSP  DP++ FP+ P      H
Sbjct: 246 VEVPPGRLCASVTYRSDLSDFNLGAHITLPPRIITDYVGSPATDPMRFFPS-PGRSVEGH 305

Query: 306 ------GSP--SSLPFSRRHSWSYDRFRP----SPPSVSFSPSPTHSESHALISNPAFPR 365
                 G P  +     R HSW+    RP    + P+ SFSP+ +H              
Sbjct: 306 SFTGRAGRPPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSH-------------- 365

Query: 366 LPPSSLPSQLPEMVTGHKDNMNYDEYYPSPVFSQSPSLSPPIRIRVKRLPNGLLQSESAP 425
                L   L +      D    D +  SP FS S S S P  I     P   ++  +AP
Sbjct: 366 ----QLSPGLHDFHWSRTDAFG-DNHQLSPPFSPSGSPSTPRYISGGNSPRINVRPGTAP 425

Query: 426 PSAPIAKLPHSPALS--SKPNLPPSPPLKASGAIISRINRDVGPLPAGSAIGKSSSLGRD 485
            + P +   +    S  S+P   P PP        S  ++D  P   G A+ +SS  G  
Sbjct: 426 VTIPSSATLNRYVSSNFSEPGRNPLPPFSPKSTRRSPSSQDSLP---GIALYRSSRSGES 485

Query: 486 ----------------------------------ESQRISGWRVSSNNSP---ISRSSSR 545
                                             +S R SG  +SS++SP    SRS SR
Sbjct: 486 PSGLMNQYPTQKVMLLTSYVSCSMQEKYHYIFKYDSGRFSG-VLSSSDSPRFAFSRSPSR 545

Query: 546 -SFPDDLDDPEFPCPFDVDE-DEMTDRGSRPESFDQKGNLCDMLEPGGFFPI--RKSQDA 605
            S  DDLDDP+  CPFD D+ DE   + S   S D++     + +     P+  R SQDA
Sbjct: 546 LSSQDDLDDPDCSCPFDFDDVDESGLQYS--HSLDRRKTSSSISQS---LPLGRRSSQDA 605

Query: 606 AVGALVRMLQKAPPLRQDFTNSTADLGQPPTPDSPSRNIQRGNQISESLALKSRYAPSSS 640
           AVG LV ML+ APPLRQD +   A +            +QR   +S +            
Sbjct: 606 AVGVLVHMLKTAPPLRQDSSTYMASMS----------GVQREGSVSGT------------ 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4J8V51.3e-16254.56Autophagy-related protein 13b OS=Arabidopsis thaliana OX=3702 GN=ATG13B PE=2 SV=... [more]
Q9SCK02.1e-5933.04Autophagy-related protein 13a OS=Arabidopsis thaliana OX=3702 GN=ATG13A PE=1 SV=... [more]
Q6C3152.6e-1728.07Autophagy-related protein 13 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX... [more]
Q5A1Z55.5e-1225.72Autophagy-related protein 13 OS=Candida albicans (strain SC5314 / ATCC MYA-2876)... [more]
Q6BQ202.1e-1123.44Autophagy-related protein 13 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 7... [more]
Match NameE-valueIdentityDescription
XP_004148643.10.0100.00autophagy-related protein 13b [Cucumis sativus] >KAE8647466.1 hypothetical prote... [more]
XP_008441017.10.098.45PREDICTED: autophagy-related protein 13b isoform X1 [Cucumis melo][more]
XP_038882060.10.094.41autophagy-related protein 13b [Benincasa hispida][more]
KAG7034429.10.089.41Autophagy-related protein 13b, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6604273.10.089.25Autophagy-related protein 13b, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A1S3B2G30.098.45autophagy-related protein 13b isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485257... [more]
A0A6J1IKL70.089.25autophagy-related protein 13b OS=Cucurbita maxima OX=3661 GN=LOC111477911 PE=4 S... [more]
A0A6J1GDD70.088.94autophagy-related protein 13b OS=Cucurbita moschata OX=3662 GN=LOC111452961 PE=4... [more]
A0A6J1BUH20.087.81autophagy-related protein 13b OS=Momordica charantia OX=3673 GN=LOC111005657 PE=... [more]
A0A0A0KFR20.0100.00ATG13 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484560 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G18770.19.3e-16454.56Autophagy-related protein 13 [more]
AT3G49590.21.5e-6033.04Autophagy-related protein 13 [more]
AT3G49590.11.5e-6033.04Autophagy-related protein 13 [more]
AT3G49590.31.4e-5831.89Autophagy-related protein 13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018731Autophagy-related protein 13, N-terminalPFAMPF10033ATG13coord: 19..258
e-value: 3.1E-42
score: 144.9
IPR036570HORMA domain superfamilyGENE3D3.30.900.10HORMA domaincoord: 3..261
e-value: 1.7E-58
score: 200.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 462..490
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..341
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 403..527
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 563..586
IPR040182Autophagy-related protein 13PANTHERPTHR13430UNCHARACTERIZEDcoord: 8..641

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G1706Cucsat.G1706gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G1706.T4.E1Cucsat.G1706.T4.E1exon
Cucsat.G1706.T4.E2Cucsat.G1706.T4.E2exon
Cucsat.G1706.T4.E3Cucsat.G1706.T4.E3exon
Cucsat.G1706.T4.E4Cucsat.G1706.T4.E4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G1706.T4.C3Cucsat.G1706.T4.C3CDS
Cucsat.G1706.T4.C2Cucsat.G1706.T4.C2CDS
Cucsat.G1706.T4.C1Cucsat.G1706.T4.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G1706.T4Cucsat.G1706.T4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0006914 autophagy
cellular_component GO:1990316 Atg1/ULK1 kinase complex