Cucsat.G1612.T8 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G1612.T8
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptiondnaJ protein P58IPK homolog
Locationctg1000: 538386 .. 543627 (+)
RNA-Seq ExpressionCucsat.G1612.T8
SyntenyCucsat.G1612.T8
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAGATGAATTAAACTTAAGAACTTATTCTAATTCTGGTCATCAAACTTGAGGAAACAATGCTGAGATTTTCGATTTCGCTATCCGCCATGGAAGCTTCAGCTTGGAGAGGATTAGCCTTCACCGTGTTTCTTCTCAACTTCGTTCTAGTTTGTCAGCTTCTTCTTCTTCAACCTTTGGTTTCGGCTTCAGGTTCGTTAATGCTACTCTCTGATCTATCTTTCTGATCTTCGCTGGAAATATTTAACTAGACGGAGTTTCTATTATTGCACTTTGGTTTGTTACCTTCGATTAGATTCCTAGATTAAGTTCTTGAATGTGCGATATTTCTTCTTGTTCTTTATTTGTTCACTGTGTAATTAATTTACGATGTTGATGAGTCTGATTTCATAGTTTGAAGCTATTACGATGACTGATGCAATTTTAATTTGGAAATGTTAATTTCTCTTATAGTTCCTCCGGCCTCCGTATTTTCTGTTCCCACATTTGTTGGTCCGAACAGTTCTGGTATTATTGATTTCTCTAAAATAATAAAGATTGGCCGCGAAATTTAAATATCGCATTCCATCTATTAATGGTACATATTTTGATTTGGAAATGGAAGGAGCCACTCTGTTTAAAGTTATTTTCAGTACCTTATTGTAAGGTCGTAGGTTTCGATGTTGTCTGATTTAAATTCTTGAGGTCATTGTCTCAGATTTAATAAGTTTCCTATGGTAGAACACAAATCGCCTAAGATTAATGGTAAACAACTCGGTCACCATACTTTAGAAAGAAGCCATTTCTTTGTTTTTCAAATTAGTGCTATGAGGAGGCTTTGAAACCTGCCTATTATATCGTCAAATGATGCCCCCGTAGTATAGAATCTATAGATCTTTGTGAGCTTTCTAGTTGTATTGTTTGATGTGTGTAAAGTCAGTAGCTCTGTCTTATCTCTACTGATCTCTCGACTTTTCTCTCATTTGATGATCTAAATGCCTTTATTTCCATTATATGGTGGGAATAATAATCATAATCAGGGAACTCAGCCTTAGAAATATTATAAGGAAGGGGCTTTCTTTGGTATTAAGTATTTACTATGCGTGATCACGAAACTCATCTTTAACCTAGGCACATAAAATTTCTAAGGAAACATGAGATTGCTCCTCGGGATGTAGCACATTTGGTTTCTCCATTATCTGCCTGATGCAAGGTTTCTTCATCTGGGGTTTGCATTTGTGGTTTTCAATTTATATTGCCAGTTGTATTTTGTAATTGATTGACTTATATCTTCAGATGGATCACTGGGTAACTCTGCCGAATTGTTTGAGAAAGTTTCACAAAATGTAAAGGTGAAGCGTTATAGTGAGGCACTCGATGATCTTAATGCTGCTATTGAGGCAGACCCAAAACTTTCAGAAGCATACTTCCATCGTGGTTCAATTCTTCGCAAGTTATGCAGGTTTTCTGCCTCTCTTGTTCTTGAAAATCATTGCAAATTATGACATCAAATCAGAAAATATGTGTAATTTTGGCATTGCGAATTAAAATCTTTCAGAATTTTGTGGATTGCAAATTAGAAACTTGGAATTCTCTGAAATGGAAATCTGCTTTGATTTGTAATATACTTCGTGCCTGTTTATGGCTGAGGTGTAAAACTGCCTCCATTACTTTTTACCATCGCATCTCCCTATGGTTACACGTCTTTGTTGTCGTAAAAACTTCCTTTCCAGATATGCTTTGATCTATTTTTCATTATGAGGAGCATATGTTGAGAATGGATATATTACAAATGAGAATAGGAAAAATTATCGGCGAAAGTAATGAGAATGGAAAATATTTCAGCTTCTCATAATTATAGCAGAAAAGAAATTTTGTATTTATTTGTGCCAAAGAGGGTAGGAGGGATCATCTCGATGGAAAATAAAAATAAACAAAAAGGGCATTTTCCCATAGAAGGTTGGACATACTTTTGAGATGAGAACATTGTAAAGGAAATTTTAAGCCATTTTGGCATGTAGAAAACCGTACTCCCGCTCTCTACCTTCCATTCTGATTGGCCAATTATATGATTCAGGAACTTTTTGGCTGATGTAGATATAATGAATCTGAGGAGAACTACAAGAAGTATCTTGAGTTAAAACCTGGAAATCCAGCGGGTGAAAAGGAGCTTTCTCAATTGCTTCAGGCTCAGAGTGCTTTAGAAACGGCTCTTAAGCATTTTGATACAGGGGATCACACAAAAGCATTGGAATTTCTGGATAAAGTTGTACTTGTTTTCTCTCCTGCATGCTCTGAGGTGATACCATCGTTATCCAATTAGACTTTTCCGTACTTTTAAACATCCTGAAATTGGTACTTATCTTTCATATGGATTTGATGTAGGCCAAACTCCTGAAGGTAAAACTGTTGTTGGCAACTAGGGACTATTCTGCTGCTATCCTTCATACTGGATATATTCTTAAAGAAGATGAAAACAATCTGGATGCATTACTACTTCGTGGGCGTGCCTACTACTATTTGGCAGATCATGATGTTGCCTCGAGGTTGATTTGACCGTACTTGTTGAAAGCATTACTTAATAAAAATGAACATGTTTTAATACGTGAACAACTTTCCCAGGCATTTTCAGAAGGGTCTTCGTCTAGATCCAGAGCATGTTGAACTGAAGAAAGCATATTTTGGGTTGAAGAATCTTCTCAAGAAAACCAAGAGTGTAAGCCCAAACTACATAAAAAGGATCTATTAATTTCCATTTTCTTGGATTTTCTTCTTTGATTGGGATAGGGAAGCTCACGGCTATAGAAATCTCTTTAGACCTTTTTTTCAGGTCTGGTAAAAGTTTTTGAGTTATTGAAATTGATAATGTTTTTCACTTTAGTTTCTATACTTTGTTGACAGGCAGAAGATAATGTCAACAAGGGTAAGCTGCGACTAGCAGTGGAGGAATATAATGCAGCCCTTGCATTGGACCCCAAACATCTTGCACACAATGTACATCTTCATCTTGGTTTATGCAAGGTACTAGTCAAGCTTGGTCGTGGAAAGGATGCTGTTACGAGCTGCAATGAAGCACTGAACATTGATGGAGACCTAATTGAAGCTTTGGTCCAGGTTCGCCTTTTCTTCTTGGCCCTCATCTCTCTGGCTCTCTTCCTTTAATATGGACTTTGTTTTGCTGAAGTGGATGTTGCAATTTTCTATATTAGTTTGTCTATAGAAAGCTCCACTGTTTCACCTTCGCAATGCTCGACCTTTTATGTTGGGCTATGATTATCATAGTTTTCATTTGCTTCCAAGACCTTTATTATTGTTCACTGTTGGAAACTCAAGCAGCAGCAGCTATCCTATCTCTCATCCTAACTTCATTTTCAATTTTACGTTAATGTTGAAGTTTGTTAGTGCATGTGTATAACAGTATAAAATCTCAAGTTCTTTAATTCTCTGAACTTTCTTCTCAGAGAGGTGAAGCAAAGCTTTTAACAGAGGACTGGGAGGGTGCTGTTGAAGATCTTAAGTCAGCGGCTCAAAGTTCACCGCAGGTTAGCTATCATTCCATTTATCCTTCAACTCTCGAAAACCTATTATTCAGTTGTGGAAGTGGTAGACAAAACTTTTTGTAAAATTTTATTCCCTTTAACGCATCAACTAATCTTATTAGAGACTTTGTTTATTGATGTTGATATTAGGATACCACCTAACAAGGAGACACTCAAGAACACTGAATTATGGAATTATATTGACAACATGCTATAAAATCATAAGAAAAGTAATGAGGTAACCCTAGCCTTTCAGGCTAGACTCCCAAATTTCCAACATTGAAAATCCCACGACATACTTTCCCTTACTCTCAACCCAAAACCTTTATTTATAACTAAAAAGCCTTAGCAAACTTACTAGCATATTTACTAGTATTCCCCTCCTAATAACCATACCAATATTCTTTTAATATGTCCATAACTAGGAGTGTTACAGGTGATCTATGTATCAAAGAAACAAAAACAGCTTATTTCTTATTATTTTTTCTCTAAAAGAACAACTTACAGTTTTTGTTAGATTTAAAAATGGGATACGTTTGTTGGGATAACTTTCATACCAGTTCATATTAATAATAAAAATCATAGTAAGACGAAATAAAACCGGGTTGAGTTTGATTCATTGCAGGTTTTGCACTGTGGGTTATCAGTAATTTGATCAATTATGTTTGCAGGATATGAATATTAGGGAGGCACTGATGAGGGCTGAAAAGGCTTTAAAAATGAGCAAACGAAAGGACTGGTACAAAATTTTAGGAGTCTCAAAGACTGCTTCTGTCGCTGAGATTAAACGTGCCTATAAAAAGCTTGCTTTGCAATGGCACCCTGACAAAAATGTGGAAAATAGAGAAGAAGCAGAAGCCAAGTTCCAAGATATTGCTGCTGCATATGAGGTCAAAATATCTATATGTTCTCATCCATTAAATAATACCAGAGTTCCAATTCTATATGCTGAAATTGATTACATGATGAATCCTTCGCCTGTCCAAAATTGGTCTTTAGTACTGTTAAAAACGTGATACTAATGCAAGATGGGTAAACATGTAGCTGAGTGGCACAAGTTATGTCTTGCAGGTCCTGGGCAATGAAGAAAAGCGTACAAGATTTGATAGAGGGGAAGACATTGAAGACATGGGCATGGGTGGCGGCGGTGGTGGCGGTTTCAATCCTTTTGGTGGTGGAGGACAGCAATTTACATTTACCTTCGACGGTGGGTTTCCTGGTGGTGGTGGTTTTGGTGGAGGTGGGTTTCCTGGTGGTGGCGGCTTTCCTGGTGGTGGTGGGTTTGAGTTCCATTTCTAATTTCAGCATAAAAGCACCAGTTCCTCTTTTTTCTGATATTTTTTTCACTGACTAGAAAACAATGATTATAAAGAGGAGGGGAAGGGGGATCAAAATATTTATTTGCTGAAAAGTAGAAATACTTTAGGTTAGAATTACTGATAGAGGCTTTTTAGTTCAGAAAAATGTTAATTGAGCAAGATTTTGTTAAAACATATTGAGAGGAAATGCATGAGTTGTAATTGTAATACGTTGGATGTATAACTATAAGCCTGCATTTATACTATTGCTTAGATTTTTTTTGCCACTTAACAAGGTATGTAATGTAAAGAGGTAGCTTTGGTAAGTGGGTTTAGTTTAGTGGTATTGATATGGTTTTTCCATACTATAATTCTTGTACTAAGAAAGCGTAGCTTTCTCTCTTTCTCTCTTTCTCTCTTTCTCTA

Coding sequence (CDS)

ATGCTGAGATTTTCGATTTCGCTATCCGCCATGGAAGCTTCAGCTTGGAGAGGATTAGCCTTCACCGTGTTTCTTCTCAACTTCGTTCTAGTTTGTCAGCTTCTTCTTCTTCAACCTTTGGTTTCGGCTTCAGATGGATCACTGGGTAACTCTGCCGAATTGTTTGAGAAAGTTTCACAAAATGTAAAGGTGAAGCGTTATAGTGAGGCACTCGATGATCTTAATGCTGCTATTGAGGCAGACCCAAAACTTTCAGAAGCATACTTCCATCGTGGTTCAATTCTTCGCAAGTTATGCAGATATAATGAATCTGAGGAGAACTACAAGAAGTATCTTGAGTTAAAACCTGGAAATCCAGCGGGTGAAAAGGAGCTTTCTCAATTGCTTCAGGCTCAGAGTGCTTTAGAAACGGCTCTTAAGCATTTTGATACAGGGGATCACACAAAAGCATTGGAATTTCTGGATAAAGTTGTACTTGTTTTCTCTCCTGCATGCTCTGAGGCCAAACTCCTGAAGGTAAAACTGTTGTTGGCAACTAGGGACTATTCTGCTGCTATCCTTCATACTGGATATATTCTTAAAGAAGATGAAAACAATCTGGATGCATTACTACTTCGTGGGCGTGCCTACTACTATTTGGCAGATCATGATGTTGCCTCGAGGCATTTTCAGAAGGGTCTTCGTCTAGATCCAGAGCATGTTGAACTGAAGAAAGCATATTTTGGGTTGAAGAATCTTCTCAAGAAAACCAAGAGTGCAGAAGATAATGTCAACAAGGGTAAGCTGCGACTAGCAGTGGAGGAATATAATGCAGCCCTTGCATTGGACCCCAAACATCTTGCACACAATGTACATCTTCATCTTGGTTTATGCAAGGTACTAGTCAAGCTTGGTCGTGGAAAGGATGCTGTTACGAGCTGCAATGAAGCACTGAACATTGATGGAGACCTAATTGAAGCTTTGGTCCAGAGAGGTGAAGCAAAGCTTTTAACAGAGGACTGGGAGGGTGCTGTTGAAGATCTTAAGTCAGCGGCTCAAAGTTCACCGCAGGATACCACCTAA

Protein sequence

MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQDTT
Homology
BLAST of Cucsat.G1612.T8 vs. ExPASy Swiss-Prot
Match: Q9LYW9 (DnaJ protein P58IPK homolog OS=Arabidopsis thaliana OX=3702 GN=P58IPK PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 2.9e-140
Identity = 254/340 (74.71%), Postives = 291/340 (85.59%), Query Frame = 0

Query: 9   SAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYS 68
           S M+  AWRG+A+T+ LLNFV  CQLLLLQPLVSA DG   ++AELFE+ SQ++KVKRYS
Sbjct: 8   SGMDFLAWRGMAYTLLLLNFVFACQLLLLQPLVSALDGQSVDAAELFERASQSIKVKRYS 67

Query: 69  EALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQL 128
           +ALDDLNAAIEADP LSEAYF R S+LR  CRY +SE +Y+KYLE K G+   EKELSQL
Sbjct: 68  DALDDLNAAIEADPALSEAYFKRASVLRHFCRYEDSENSYQKYLEFKSGDSNAEKELSQL 127

Query: 129 LQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILH 188
            QA+SALETA   +++ D  KALEF+DKVVLVFSPACS+AKLLKVKLL+ ++DYS AI  
Sbjct: 128 HQAKSALETASTLYESKDIAKALEFVDKVVLVFSPACSKAKLLKVKLLMVSKDYSGAISE 187

Query: 189 TGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLK 248
           TGYILKEDENNL+ALLLRGRAYYYLADHD+A RH+QKGLRLDPEH ELKKAYFGLK LLK
Sbjct: 188 TGYILKEDENNLEALLLRGRAYYYLADHDIAQRHYQKGLRLDPEHSELKKAYFGLKKLLK 247

Query: 249 KTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCN 308
           KTKSAEDN NKGKLR++ EEY  A+ALDP+H A+NVHL+LGLCKV V+LGRGKD + SCN
Sbjct: 248 KTKSAEDNANKGKLRVSAEEYKEAIALDPEHTANNVHLYLGLCKVSVRLGRGKDGLNSCN 307

Query: 309 EALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSS 349
           EALNID +LIEAL QRGEAKLL EDWEGAVEDLK AAQ+S
Sbjct: 308 EALNIDAELIEALHQRGEAKLLLEDWEGAVEDLKQAAQNS 347

BLAST of Cucsat.G1612.T8 vs. ExPASy Swiss-Prot
Match: Q5JNB5 (DnaJ protein P58IPK homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=P58B PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.0e-118
Identity = 216/323 (66.87%), Postives = 263/323 (81.42%), Query Frame = 0

Query: 29  VLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEALDDLNAAIEADPKLSEAY 88
           VL+  LLL    V A +G   + + LF++ S+ + +++Y  +L  LNA +E DP  SEAY
Sbjct: 10  VLLPLLLLHSSPVFAQEGQDNDPSTLFKRASEMMNLRKYDGSLGLLNAVLEVDPNHSEAY 69

Query: 89  FHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQLLQAQSALETALKHFDTGDHT 148
             R S+LR  CRY E+E +Y KYLELKPG+ + EKELSQLLQAQ+ALE+A   F++ D +
Sbjct: 70  RQRASVLRHKCRYKEAEGDYSKYLELKPGSSSVEKELSQLLQAQNALESAYGQFESHDFS 129

Query: 149 KALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTGYILKEDENNLDALLLRGR 208
           K LE+++K+VLVFSP C +AKLLK K LLA  DYS+ I  TG+ILKEDE+NLDALLLRGR
Sbjct: 130 KVLEYINKIVLVFSPNCLKAKLLKAKALLALEDYSSVISETGFILKEDEDNLDALLLRGR 189

Query: 209 AYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKTKSAEDNVNKGKLRLAVEE 268
           AYYYLADHDVASRH+QKGLRLDPEH ELKKAYFGLKNLLKKTKSAEDN  KGKLR++ E+
Sbjct: 190 AYYYLADHDVASRHYQKGLRLDPEHSELKKAYFGLKNLLKKTKSAEDNAAKGKLRVSAED 249

Query: 269 YNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEALNIDGDLIEALVQRGEAK 328
           Y AALA+DP H ++NVHL+LGLCKVLVKLGRGK+A++SC EALNIDG+L++AL QRGEAK
Sbjct: 250 YKAALAMDPDHTSYNVHLYLGLCKVLVKLGRGKEAISSCTEALNIDGELVDALTQRGEAK 309

Query: 329 LLTEDWEGAVEDLKSAAQSSPQD 352
           LLTEDWEGAV+DLK A+Q SPQD
Sbjct: 310 LLTEDWEGAVQDLKEASQKSPQD 332

BLAST of Cucsat.G1612.T8 vs. ExPASy Swiss-Prot
Match: A0A0P0VG31 (DnaJ protein P58IPK homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=P58A PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.1e-115
Identity = 207/311 (66.56%), Postives = 256/311 (82.32%), Query Frame = 0

Query: 41  VSASDGSLGNSAELFEKVSQNVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCR 100
           V A +G   + + LF++  + + +++Y  +L  LNA +E +P  SEAY  R S+LR  CR
Sbjct: 26  VFAQEGQDNDPSTLFKRALEMMNLRKYDGSLGLLNAVLEVEPNHSEAYRQRASVLRHKCR 85

Query: 101 YNESEENYKKYLELKPGNPAGEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLV 160
           Y E+E +Y KYLELKPG+ + EKELSQLLQAQ+ALE+A   F++ D +K L++++K+VLV
Sbjct: 86  YKEAEGDYSKYLELKPGSSSVEKELSQLLQAQNALESAYGQFESHDFSKVLDYINKIVLV 145

Query: 161 FSPACSEAKLLKVKLLLATRDYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVAS 220
           FSP C +AKLLK K LLA +DYS  I  TG+ILKEDE+NLDALLLRGRAYYYLADHDVAS
Sbjct: 146 FSPDCLKAKLLKAKALLALKDYSTVISETGFILKEDEDNLDALLLRGRAYYYLADHDVAS 205

Query: 221 RHFQKGLRLDPEHVELKKAYFGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHL 280
           RH+QKGLRLDPEH ELKKAYFGLKNL+KKTKSAEDN  KGKLR++ E+Y A+LA+DP H 
Sbjct: 206 RHYQKGLRLDPEHSELKKAYFGLKNLVKKTKSAEDNAAKGKLRVSAEDYKASLAMDPDHT 265

Query: 281 AHNVHLHLGLCKVLVKLGRGKDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVED 340
           ++NVHL+LGLCKVLVKLGRGK+A++SC EALNIDG+L++AL QRGEAKLLTEDWEGAV+D
Sbjct: 266 SYNVHLYLGLCKVLVKLGRGKEAISSCTEALNIDGELVDALTQRGEAKLLTEDWEGAVQD 325

Query: 341 LKSAAQSSPQD 352
           LK AAQ SPQD
Sbjct: 326 LKEAAQKSPQD 336

BLAST of Cucsat.G1612.T8 vs. ExPASy Swiss-Prot
Match: Q54M21 (DnaJ homolog subfamily C member 3 homolog OS=Dictyostelium discoideum OX=44689 GN=dnajc3 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.6e-29
Identity = 79/283 (27.92%), Postives = 147/283 (51.94%), Query Frame = 0

Query: 70  ALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQLL 129
           AL DLN AIEA+P    A   R  I   L R+ E+ + YK+ L+++P N   ++++ +L 
Sbjct: 88  ALSDLNRAIEANPDNIHARLKRAKIQSSLGRFEEAMDEYKRVLKIRPDNSQAKQQIEKLK 147

Query: 130 QAQSALETALKHFDTGDHTK-ALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILH 189
           + +  LE          + K ++  L  +  V S    E +L+  +      D+   +  
Sbjct: 148 KVEQQLEKVRDMVKVEKNYKDSIAILLDIQSVVSD-LKEVRLMLCECFFQQGDHRKVLDE 207

Query: 190 TGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLK 249
           T  ILK + +++ AL  RG+ ++ + + ++A +  ++GL+ DP++   +     +    K
Sbjct: 208 TMTILKSEPSSVAALYWRGKTFFSMGEKEIAMKFLKEGLKFDPDNTNCRAMIKTINKFEK 267

Query: 250 KTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCN 309
            T +A++  N+ K + A+ +   AL ++P    H+  L+L  CK L+K+ +GK+++ +CN
Sbjct: 268 STANAQELFNQQKYQDALGQIEDALEIEPNSPTHSTPLYLLKCKCLLKVKKGKESIEACN 327

Query: 310 EALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 352
            AL +D    +AL  R EA +  ED++ A+ D   A +  P D
Sbjct: 328 RALELDELNADALYNRAEAYMYEEDYQKALNDYNKAREHKPND 369

BLAST of Cucsat.G1612.T8 vs. ExPASy Swiss-Prot
Match: Q13217 (DnaJ homolog subfamily C member 3 OS=Homo sapiens OX=9606 GN=DNAJC3 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 7.0e-25
Identity = 83/316 (26.27%), Postives = 152/316 (48.10%), Query Frame = 0

Query: 42  SASDGSLGNSAELFEKVSQNVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRY 101
           +A DG   N    + + +  + + +   AL DL   I+     + A   RG +L K  + 
Sbjct: 62  AAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKL 121

Query: 102 NESEENYKKYLELKPGNPAGEKELSQLLQA---QSALETALKHFDTGDHTKALEFLDKVV 161
           +E+E+++KK L+  P     ++  SQL+++   Q     AL  F +GD+T A+ FLDK++
Sbjct: 122 DEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKIL 181

Query: 162 LVFSPAC---SEAKLLKVKLLLATRDYSAAILHTGYILKEDENNLDALLLRGRAYYYLAD 221
            V    C   +E + L+ +  +   +   AI       K   +N +A       YY L D
Sbjct: 182 EV----CVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGD 241

Query: 222 HDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALAL 281
           H+++    ++ L+LD +H      Y  +K L K  +SAE+ +  G+   A  +Y + +  
Sbjct: 242 HELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKT 301

Query: 282 DPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWE 341
           +P    + V     +C    K  +  +A+  C+E L ++ D + AL  R EA L+ E ++
Sbjct: 302 EPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYD 361

Query: 342 GAVEDLKSAAQSSPQD 352
            A++D ++A + +  D
Sbjct: 362 EAIQDYETAQEHNEND 373

BLAST of Cucsat.G1612.T8 vs. NCBI nr
Match: XP_004143240.1 (dnaJ protein P58IPK homolog [Cucumis sativus] >KGN48138.1 hypothetical protein Csa_003500 [Cucumis sativus])

HSP 1 Score: 674 bits (1739), Expect = 1.53e-241
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ
Sbjct: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA
Sbjct: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR
Sbjct: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY
Sbjct: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG
Sbjct: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351

BLAST of Cucsat.G1612.T8 vs. NCBI nr
Match: XP_008449262.1 (PREDICTED: dnaJ protein P58IPK homolog isoform X1 [Cucumis melo])

HSP 1 Score: 667 bits (1720), Expect = 1.43e-238
Identity = 346/351 (98.58%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSISLSAMEASAWRGLAFT FLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ
Sbjct: 7   MLRFSISLSAMEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 66

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESE+NYKKYLELKPGNPA
Sbjct: 67  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEKNYKKYLELKPGNPA 126

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETAL+HFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR
Sbjct: 127 GEKELSQLLQAQSALETALRHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 186

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY
Sbjct: 187 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 246

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTKSAEDNVNKGKLR+AVEEYNAALALDP HLAHNVHLHLGLCKVLVKLGRG
Sbjct: 247 FGLKNLLKKTKSAEDNVNKGKLRVAVEEYNAALALDPNHLAHNVHLHLGLCKVLVKLGRG 306

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 307 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 357

BLAST of Cucsat.G1612.T8 vs. NCBI nr
Match: KAA0047271.1 (dnaJ protein P58IPK-like protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 651 bits (1680), Expect = 3.42e-233
Identity = 336/341 (98.53%), Postives = 339/341 (99.41%), Query Frame = 0

Query: 11  MEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA 70
           MEASAWRGLAFT FLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA
Sbjct: 1   MEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA 60

Query: 71  LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQLLQ 130
           LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESE+NYKKYLELKPGNPAGEKELSQLLQ
Sbjct: 61  LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEKNYKKYLELKPGNPAGEKELSQLLQ 120

Query: 131 AQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG 190
           AQSALETAL+HFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG
Sbjct: 121 AQSALETALRHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG 180

Query: 191 YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT 250
           YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT
Sbjct: 181 YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT 240

Query: 251 KSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA 310
           KSAEDNVNKGKLR+AVEEYNAALALDP HLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA
Sbjct: 241 KSAEDNVNKGKLRVAVEEYNAALALDPNHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA 300

Query: 311 LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 341

BLAST of Cucsat.G1612.T8 vs. NCBI nr
Match: XP_038883694.1 (dnaJ protein P58IPK homolog [Benincasa hispida])

HSP 1 Score: 641 bits (1654), Expect = 1.10e-228
Identity = 333/351 (94.87%), Postives = 342/351 (97.44%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           ML FSISLSAMEASAWRG+AFTVFLLNFVLVCQLLLLQPLVSASDG LGNSAELFEKVSQ
Sbjct: 7   MLGFSISLSAMEASAWRGVAFTVFLLNFVLVCQLLLLQPLVSASDGILGNSAELFEKVSQ 66

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NVKVKRYSEALDDLNAAIE DPKLSEAYFHRGSILR+LCRYNESE+NYKKYLELKPGN  
Sbjct: 67  NVKVKRYSEALDDLNAAIEIDPKLSEAYFHRGSILRQLCRYNESEKNYKKYLELKPGNSV 126

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALKHFDTGD+TKALEFLDKVVLVFSPACSEAK LK+KLLLAT+
Sbjct: 127 GEKELSQLLQAQSALETALKHFDTGDYTKALEFLDKVVLVFSPACSEAKFLKIKLLLATK 186

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAILHTGYILKEDE+NLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEH ELKKAY
Sbjct: 187 DYSAAILHTGYILKEDESNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHGELKKAY 246

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKT+SAEDNV+KGKLRLAVEEYNAALALDP HLAHNVHLHLGLCKVLVKLGRG
Sbjct: 247 FGLKNLLKKTRSAEDNVSKGKLRLAVEEYNAALALDPNHLAHNVHLHLGLCKVLVKLGRG 306

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAV SCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 307 KDAVASCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 357

BLAST of Cucsat.G1612.T8 vs. NCBI nr
Match: XP_023543086.1 (dnaJ protein P58IPK homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625 bits (1613), Expect = 1.34e-222
Identity = 325/351 (92.59%), Postives = 339/351 (96.58%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSI L AMEASAWRGLAFT FLLNFVLVCQLLLLQPLVSA+DG LGNSAELF+KVSQ
Sbjct: 1   MLRFSIPLPAMEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSAADGKLGNSAELFKKVSQ 60

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NV+VKRYSEALDDLNAAIEADP+LSEAYFHRGSILR+LCRYNESE++YKKYLELKPGNPA
Sbjct: 61  NVRVKRYSEALDDLNAAIEADPELSEAYFHRGSILRQLCRYNESEKSYKKYLELKPGNPA 120

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALK FDTGDHTK+LEFLDKVVLVFSPACSEAK LK+KLLLAT+
Sbjct: 121 GEKELSQLLQAQSALETALKLFDTGDHTKSLEFLDKVVLVFSPACSEAKFLKIKLLLATK 180

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAIL TGYILKEDE+NLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEH ELKKAY
Sbjct: 181 DYSAAILETGYILKEDESNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHGELKKAY 240

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTK AEDNV+KGKLRLAVEEY AALALDP HLAHNVHLHLGLCKVLV LGRG
Sbjct: 241 FGLKNLLKKTKIAEDNVSKGKLRLAVEEYYAALALDPNHLAHNVHLHLGLCKVLVNLGRG 300

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSC+EAL+IDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 KDAVTSCSEALSIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351

BLAST of Cucsat.G1612.T8 vs. ExPASy TrEMBL
Match: A0A0A0KF24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445030 PE=4 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 7.41e-242
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ
Sbjct: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA
Sbjct: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR
Sbjct: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY
Sbjct: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG
Sbjct: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351

BLAST of Cucsat.G1612.T8 vs. ExPASy TrEMBL
Match: A0A1S3BLN0 (dnaJ protein P58IPK homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491196 PE=4 SV=1)

HSP 1 Score: 667 bits (1720), Expect = 6.93e-239
Identity = 346/351 (98.58%), Postives = 349/351 (99.43%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSISLSAMEASAWRGLAFT FLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ
Sbjct: 7   MLRFSISLSAMEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 66

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESE+NYKKYLELKPGNPA
Sbjct: 67  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEKNYKKYLELKPGNPA 126

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETAL+HFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR
Sbjct: 127 GEKELSQLLQAQSALETALRHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 186

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY
Sbjct: 187 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 246

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTKSAEDNVNKGKLR+AVEEYNAALALDP HLAHNVHLHLGLCKVLVKLGRG
Sbjct: 247 FGLKNLLKKTKSAEDNVNKGKLRVAVEEYNAALALDPNHLAHNVHLHLGLCKVLVKLGRG 306

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 307 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 357

BLAST of Cucsat.G1612.T8 vs. ExPASy TrEMBL
Match: A0A5A7U102 (DnaJ protein P58IPK-like protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00640 PE=4 SV=1)

HSP 1 Score: 651 bits (1680), Expect = 1.66e-233
Identity = 336/341 (98.53%), Postives = 339/341 (99.41%), Query Frame = 0

Query: 11  MEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA 70
           MEASAWRGLAFT FLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA
Sbjct: 1   MEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYSEA 60

Query: 71  LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQLLQ 130
           LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESE+NYKKYLELKPGNPAGEKELSQLLQ
Sbjct: 61  LDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEKNYKKYLELKPGNPAGEKELSQLLQ 120

Query: 131 AQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG 190
           AQSALETAL+HFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG
Sbjct: 121 AQSALETALRHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILHTG 180

Query: 191 YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT 250
           YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT
Sbjct: 181 YILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLKKT 240

Query: 251 KSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA 310
           KSAEDNVNKGKLR+AVEEYNAALALDP HLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA
Sbjct: 241 KSAEDNVNKGKLRVAVEEYNAALALDPNHLAHNVHLHLGLCKVLVKLGRGKDAVTSCNEA 300

Query: 311 LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 LNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 341

BLAST of Cucsat.G1612.T8 vs. ExPASy TrEMBL
Match: A0A6J1IQ04 (dnaJ protein P58IPK homolog OS=Cucurbita maxima OX=3661 GN=LOC111478880 PE=4 SV=1)

HSP 1 Score: 625 bits (1612), Expect = 9.18e-223
Identity = 325/351 (92.59%), Postives = 338/351 (96.30%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           MLRFSI L AMEASAWRGL FT FLLNFVLVCQLLLLQPLVSA+DG LGNSAELF+KVSQ
Sbjct: 1   MLRFSIPLPAMEASAWRGLEFTAFLLNFVLVCQLLLLQPLVSAADGKLGNSAELFKKVSQ 60

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NV+VKRYSEALDDLNAAIEADPKLSEAYFHRGSILR+LCRYNESE++YKKYLELKPGNPA
Sbjct: 61  NVRVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRQLCRYNESEKSYKKYLELKPGNPA 120

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALK FDTGDHTK+LEFLDKVVLVFSPACSEAK LK+KLLLAT+
Sbjct: 121 GEKELSQLLQAQSALETALKLFDTGDHTKSLEFLDKVVLVFSPACSEAKFLKIKLLLATK 180

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAIL TGYILKEDE+NLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEH ELKKAY
Sbjct: 181 DYSAAILETGYILKEDESNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHGELKKAY 240

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTK AEDNV+KGKLRLAVEEY AALALDP HLAHNVHLHLGLCKVLV LGRG
Sbjct: 241 FGLKNLLKKTKIAEDNVSKGKLRLAVEEYYAALALDPNHLAHNVHLHLGLCKVLVNLGRG 300

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSC+EAL+IDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 KDAVTSCSEALSIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351

BLAST of Cucsat.G1612.T8 vs. ExPASy TrEMBL
Match: A0A6J1EGN0 (dnaJ protein P58IPK homolog OS=Cucurbita moschata OX=3662 GN=LOC111433201 PE=4 SV=1)

HSP 1 Score: 625 bits (1611), Expect = 1.30e-222
Identity = 325/351 (92.59%), Postives = 338/351 (96.30%), Query Frame = 0

Query: 1   MLRFSISLSAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQ 60
           ML FSI L AMEASAWRGLAFT FLLNFVLVCQLLLLQPLVSA+DG LGNSAELF+KVSQ
Sbjct: 1   MLSFSIPLPAMEASAWRGLAFTAFLLNFVLVCQLLLLQPLVSAADGKLGNSAELFKKVSQ 60

Query: 61  NVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPA 120
           NV+VKRYSEALDDLNAAIEADPKLSEAYFHRGSILR+LCRYNESE++YKKYLELKPGNPA
Sbjct: 61  NVRVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRQLCRYNESEKSYKKYLELKPGNPA 120

Query: 121 GEKELSQLLQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATR 180
           GEKELSQLLQAQSALETALK FDTGDHTK+LEFLDKVVLVFSPACSEAK LK+KLLLAT+
Sbjct: 121 GEKELSQLLQAQSALETALKLFDTGDHTKSLEFLDKVVLVFSPACSEAKFLKIKLLLATK 180

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           DYSAAIL TGYILKEDE+NLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEH ELKKAY
Sbjct: 181 DYSAAILETGYILKEDESNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHGELKKAY 240

Query: 241 FGLKNLLKKTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRG 300
           FGLKNLLKKTK AEDNV+KGKLRLAVEEY AALALDP HLAHNVHLHLGLCKVLV LGRG
Sbjct: 241 FGLKNLLKKTKIAEDNVSKGKLRLAVEEYYAALALDPNHLAHNVHLHLGLCKVLVNLGRG 300

Query: 301 KDAVTSCNEALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351
           KDAVTSC+EAL+IDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD
Sbjct: 301 KDAVTSCSEALSIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSSPQD 351

BLAST of Cucsat.G1612.T8 vs. TAIR 10
Match: AT5G03160.1 (homolog of mamallian P58IPK )

HSP 1 Score: 499.6 bits (1285), Expect = 2.1e-141
Identity = 254/340 (74.71%), Postives = 291/340 (85.59%), Query Frame = 0

Query: 9   SAMEASAWRGLAFTVFLLNFVLVCQLLLLQPLVSASDGSLGNSAELFEKVSQNVKVKRYS 68
           S M+  AWRG+A+T+ LLNFV  CQLLLLQPLVSA DG   ++AELFE+ SQ++KVKRYS
Sbjct: 8   SGMDFLAWRGMAYTLLLLNFVFACQLLLLQPLVSALDGQSVDAAELFERASQSIKVKRYS 67

Query: 69  EALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENYKKYLELKPGNPAGEKELSQL 128
           +ALDDLNAAIEADP LSEAYF R S+LR  CRY +SE +Y+KYLE K G+   EKELSQL
Sbjct: 68  DALDDLNAAIEADPALSEAYFKRASVLRHFCRYEDSENSYQKYLEFKSGDSNAEKELSQL 127

Query: 129 LQAQSALETALKHFDTGDHTKALEFLDKVVLVFSPACSEAKLLKVKLLLATRDYSAAILH 188
            QA+SALETA   +++ D  KALEF+DKVVLVFSPACS+AKLLKVKLL+ ++DYS AI  
Sbjct: 128 HQAKSALETASTLYESKDIAKALEFVDKVVLVFSPACSKAKLLKVKLLMVSKDYSGAISE 187

Query: 189 TGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAYFGLKNLLK 248
           TGYILKEDENNL+ALLLRGRAYYYLADHD+A RH+QKGLRLDPEH ELKKAYFGLK LLK
Sbjct: 188 TGYILKEDENNLEALLLRGRAYYYLADHDIAQRHYQKGLRLDPEHSELKKAYFGLKKLLK 247

Query: 249 KTKSAEDNVNKGKLRLAVEEYNAALALDPKHLAHNVHLHLGLCKVLVKLGRGKDAVTSCN 308
           KTKSAEDN NKGKLR++ EEY  A+ALDP+H A+NVHL+LGLCKV V+LGRGKD + SCN
Sbjct: 248 KTKSAEDNANKGKLRVSAEEYKEAIALDPEHTANNVHLYLGLCKVSVRLGRGKDGLNSCN 307

Query: 309 EALNIDGDLIEALVQRGEAKLLTEDWEGAVEDLKSAAQSS 349
           EALNID +LIEAL QRGEAKLL EDWEGAVEDLK AAQ+S
Sbjct: 308 EALNIDAELIEALHQRGEAKLLLEDWEGAVEDLKQAAQNS 347

BLAST of Cucsat.G1612.T8 vs. TAIR 10
Match: AT4G11260.1 (phosphatase-related )

HSP 1 Score: 47.0 bits (110), Expect = 3.7e-05
Identity = 24/79 (30.38%), Postives = 44/79 (55.70%), Query Frame = 0

Query: 50  NSAELF-EKVSQNVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENY 109
           N A  F ++   N+K+  ++EA+ D N AIE +P L++AY  +G+   KL  Y+ ++   
Sbjct: 34  NCAAFFADRAQANIKIDNFTEAVVDANKAIELEPTLAKAYLRKGTACMKLEEYSTAKAAL 93

Query: 110 KKYLELKPGNPAGEKELSQ 128
           +K   + P  P  +K + +
Sbjct: 94  EKGASVAPNEPKFKKMIDE 112

BLAST of Cucsat.G1612.T8 vs. TAIR 10
Match: AT2G15790.1 (peptidyl-prolyl cis-trans isomerase / cyclophilin-40 (CYP40) / rotamase )

HSP 1 Score: 46.2 bits (108), Expect = 6.3e-05
Identity = 25/83 (30.12%), Postives = 44/83 (53.01%), Query Frame = 0

Query: 181 DYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVELKKAY 240
           D   A+L T + +++++NN+ AL  +G+AY  L + D A+   +K L+ +P    +KK Y
Sbjct: 279 DAKGALLDTEFAMRDEDNNVKALFRQGQAYMALNNVDAAAESLEKALQFEPNDAGIKKEY 338

Query: 241 FGLKNLLKKTKSAEDNVNKGKLR 264
                 + K  +  DN  K + R
Sbjct: 339 AA----VMKKIAFRDNEEKKQYR 357

BLAST of Cucsat.G1612.T8 vs. TAIR 10
Match: AT5G48570.1 (FKBP-type peptidyl-prolyl cis-trans isomerase family protein )

HSP 1 Score: 46.2 bits (108), Expect = 6.3e-05
Identity = 30/108 (27.78%), Postives = 50/108 (46.30%), Query Frame = 0

Query: 177 LATRDYSAAILHTGYILKEDENNLDALLLRGRAYYYLADHDVASRHFQKGLRLDPEHVEL 236
           L  +DY  A   +  +L+ D  N+ A+  R  AY   AD D+A    +K L +DP++ E+
Sbjct: 470 LKLKDYKEAAKLSTKVLEMDSRNVKAMYRRAHAYLETADLDLAELDIKKALEIDPDNKEV 529

Query: 237 KKAYFGLKNLLKKTKSAEDNVNKGKLRLAVEEYNA------ALALDPK 279
           K  Y  LK  +K+    +       L   +E +        A+++D K
Sbjct: 530 KIEYKKLKEKVKEYNKKDAKFYSNMLSKMLEPHKGTQKEAQAMSIDTK 577

BLAST of Cucsat.G1612.T8 vs. TAIR 10
Match: AT4G23570.1 (phosphatase-related )

HSP 1 Score: 44.7 bits (104), Expect = 1.8e-04
Identity = 24/79 (30.38%), Postives = 43/79 (54.43%), Query Frame = 0

Query: 50  NSAELF-EKVSQNVKVKRYSEALDDLNAAIEADPKLSEAYFHRGSILRKLCRYNESEENY 109
           N AE F ++    +K++ ++EA+ D N AIE DP L++AY  +G+   KL  Y  ++   
Sbjct: 34  NCAEFFADRAQAYIKLESFTEAVADANKAIELDPSLTKAYLRKGTACMKLEEYRTAKTAL 93

Query: 110 KKYLELKPGNPAGEKELSQ 128
           +K   + P     +K + +
Sbjct: 94  EKGASITPSESKFKKLIDE 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYW92.9e-14074.71DnaJ protein P58IPK homolog OS=Arabidopsis thaliana OX=3702 GN=P58IPK PE=1 SV=1[more]
Q5JNB57.0e-11866.87DnaJ protein P58IPK homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=P58B P... [more]
A0A0P0VG311.1e-11566.56DnaJ protein P58IPK homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=P58A P... [more]
Q54M213.6e-2927.92DnaJ homolog subfamily C member 3 homolog OS=Dictyostelium discoideum OX=44689 G... [more]
Q132177.0e-2526.27DnaJ homolog subfamily C member 3 OS=Homo sapiens OX=9606 GN=DNAJC3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143240.11.53e-241100.00dnaJ protein P58IPK homolog [Cucumis sativus] >KGN48138.1 hypothetical protein C... [more]
XP_008449262.11.43e-23898.58PREDICTED: dnaJ protein P58IPK homolog isoform X1 [Cucumis melo][more]
KAA0047271.13.42e-23398.53dnaJ protein P58IPK-like protein isoform X1 [Cucumis melo var. makuwa][more]
XP_038883694.11.10e-22894.87dnaJ protein P58IPK homolog [Benincasa hispida][more]
XP_023543086.11.34e-22292.59dnaJ protein P58IPK homolog [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KF247.41e-242100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445030 PE=4 SV=1[more]
A0A1S3BLN06.93e-23998.58dnaJ protein P58IPK homolog isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491196 P... [more]
A0A5A7U1021.66e-23398.53DnaJ protein P58IPK-like protein isoform X1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1IQ049.18e-22392.59dnaJ protein P58IPK homolog OS=Cucurbita maxima OX=3661 GN=LOC111478880 PE=4 SV=... [more]
A0A6J1EGN01.30e-22292.59dnaJ protein P58IPK homolog OS=Cucurbita moschata OX=3662 GN=LOC111433201 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT5G03160.12.1e-14174.71homolog of mamallian P58IPK [more]
AT4G11260.13.7e-0530.38phosphatase-related [more]
AT2G15790.16.3e-0530.12peptidyl-prolyl cis-trans isomerase / cyclophilin-40 (CYP40) / rotamase [more]
AT5G48570.16.3e-0527.78FKBP-type peptidyl-prolyl cis-trans isomerase family protein [more]
AT4G23570.11.8e-0430.38phosphatase-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 318..351
e-value: 4.9
score: 16.1
coord: 284..317
e-value: 34.0
score: 10.3
coord: 85..118
e-value: 0.0011
score: 28.3
coord: 51..84
e-value: 81.0
score: 7.0
coord: 131..164
e-value: 380.0
score: 1.1
coord: 200..233
e-value: 0.0091
score: 25.2
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 200..233
score: 10.8859
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 85..118
score: 10.3549
NoneNo IPR availablePFAMPF13432TPR_16coord: 257..311
e-value: 0.016
score: 15.9
IPR013105Tetratricopeptide repeat 2PFAMPF07719TPR_2coord: 86..118
e-value: 5.2E-5
score: 23.0
coord: 201..233
e-value: 2.7E-5
score: 23.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 55..353
e-value: 1.9E-68
score: 233.3
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 64..351
IPR044620DnaJ protein P58IPK-likePANTHERPTHR45188DNAJ PROTEIN P58IPK HOMOLOGcoord: 7..352

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G1612Cucsat.G1612gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G1612.T8.E1Cucsat.G1612.T8.E1exon
Cucsat.G1612.T8.E2Cucsat.G1612.T8.E2exon
Cucsat.G1612.T8.E3Cucsat.G1612.T8.E3exon
Cucsat.G1612.T8.E4Cucsat.G1612.T8.E4exon
Cucsat.G1612.T8.E5Cucsat.G1612.T8.E5exon
Cucsat.G1612.T8.E6Cucsat.G1612.T8.E6exon
Cucsat.G1612.T8.E7Cucsat.G1612.T8.E7exon
Cucsat.G1612.T8.E8Cucsat.G1612.T8.E8exon
Cucsat.G1612.T8.E9Cucsat.G1612.T8.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G1612.T8.C1Cucsat.G1612.T8.C1CDS
Cucsat.G1612.T8.C2Cucsat.G1612.T8.C2CDS
Cucsat.G1612.T8.C3Cucsat.G1612.T8.C3CDS
Cucsat.G1612.T8.C4Cucsat.G1612.T8.C4CDS
Cucsat.G1612.T8.C5Cucsat.G1612.T8.C5CDS
Cucsat.G1612.T8.C6Cucsat.G1612.T8.C6CDS
Cucsat.G1612.T8.C7Cucsat.G1612.T8.C7CDS
Cucsat.G1612.T8.C8Cucsat.G1612.T8.C8CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G1612.T8Cucsat.G1612.T8-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044794 positive regulation by host of viral process
cellular_component GO:0005788 endoplasmic reticulum lumen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity