Cucsat.G13206.T2 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G13206.T2
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmediator of RNA polymerase II transcription subunit 15a-like
Locationctg1838: 2203474 .. 2207899 (+)
RNA-Seq ExpressionCucsat.G13206.T2
SyntenyCucsat.G13206.T2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCCCCCTAAACCCAAAAGAAGAAACTGCTTCTACTACTCACTTCTTTCAAACTCTCCGTTTTCCTCTTCTTTCCCTCTCTTTCCCTCTCTCCATCTCCATTCTATTTCTCACTCTTTTTCTCATATCCTTCTCTCTTCACTTCCTTCAATCACTCCATCCCTTGATTCTACCTTCCTCTCTGATTATGTAGGGTTTTTTTTTTTTTTCTTTTTTTTCTTTCTGTTTGCTTACTGGACAAGAAAGCTAGTATGGCTACTCCAACTGATTGGAGGACGGAAATAACTCAAGAAACTAGACAGCAAATTGTTCGTTCAATGTAATATTTCCTAAACTTGTACTCTGCTTTATTTATTTATTTATTTTAGACTTTTTAAGTGCTGCTTATTGATGTTTGATTACGATTACTTTGTTGCCTTTTTTTTTTCTAATTTCGTTGTTGAATTGAAAATTTCGTGCATTATTGCTTGAGTTTTACTCGATTTCCATTGATTTCTTGAATTATGAATTTGTGGATTTTATTGATAATAGTATGATATTGCAGATATATGATGTTGAAGGAACAGCCTTCTGAGTTGAATGTGAAACTAATTAGTGACCGTGCGAGGAAACATGAGATGAACTTGTTTAGTACTGCCAAATCTAAGGTATTATTAGTGAATTTTTCAATGTTTTCTAATATGAAACTGATGTTTTTCGTGCATTTGTGTTTTATTGTGTACTTTCCAATATTTGTGATCTAATCTGCTTATGCTTCTCAGGAAGAATATTTGTCTACTGGAACTGGAAAGATGATTAAGAGAGAAAATCACCAGGGGAGTTCAAGCGGTCAAGCAGTAGTGGTTTATCCGCAATATCATCAGCCAGCAGAGGCTAAGTCACTTTTGCTGCAACATATTCAGCGAACGCCACAGCTACATAGACAACATCCAAATGTGAGACAGGCACATCAACAATTTGCGATGCAGAATCAAAGCGGTGCTAGTCCACAAAATACATCAAATTCACAGTGCAGACCCCAGGGTTTCCCGAGACAAGATACTGGAATTCATCTGTCCTCAGAAATGTTCACGCAACATCCTAACTTTGTGAACTTGACCACTCAAGTTAAAAAGGTATTGAATTGTGTCTAGATGTGTAAATGGGCAGTTTGCTGGTACATCTAAATTAGATATATTGTGTTATGAAATTGAAACTGTATGTAACATAACAGGAAGTGGATAGCGAAGGTTTTATGGCCTCCAAATCCTCCGAGCAGCGTAAGCAACACTCCATGAGTGGAGCTTCTGCAGGTAAGTTTTTGTAGATGCTAAGCTTCAAATCACCTGCGATGATAATAACAACTAGCAGGAAATCTGGGAGGAAACTATTGTATTGGATTCAAACAATACTACAAAGTAGTCCGGAAACTATTGTATTGGATTCAATCAATACTACAAAGTAGTCTGGAAACTATTGTATTGGATTCAATCAATACTACAAAATAGTCCCGAAACTATTGTATTGTATTCAATCAATACTACAAAATAGTTCTTCAAGCCAAATAAGAGGGCTGTACTCTCCCCAATGAACACAGGTTCATACAAAACACATAAACAAATAAGATAACCAACCCTAACTAACATGTTGTCCATGTAGAATTTCCCTTTTTACCCCTCCTCTTATACACGGGTAATGGGTGGGCTAGTAGAGTTCCTATTTTCAAACACTATAATTTTTAGCCATATATAGATTGTGGATTTAATTTTAACAACTTGTATCTTTCATTTGCCTTTTGAATTTATGATGTTTTTAAGAAAGTGTTTAGATTCTTTTGTTTCTTAAAGTTCTTGTATTCTAAGAAATATATTTTTCTGTCACTTTTCTACTTGGCTTTTGATAGCTACGTTTGAAAAACAAAATACAATCTTTCTGGAAATTATTTTTTGATAAACTTTTTGAGAAATGTGTATTTGTGCAAACGAAATTATATGTTTTACCTCTAAAAATGAGAGCCGGCAAAATCATTATATAGTGAAGATGGATACTCTGCCTGCATGACTTATGTTTAGAAACATGGAGCTCAATCTACTTTATTAGCGAATATTTGGAACCATTACTGCGGTAGTCTGCTTATTTTTCTTTTTGTTACATAGATCCAGAAAGAATTCCAAATTCAGAAGTTTGGCATGATGCTGCTTTTGCTGAGGTAATTTTTAGTTATGAATATGAGTGAAAAATGGGAAAGGAGGTGGAAAAATATTGGCAGCCGTTGAAGATGACCAGGGTAAGAGTAACAAGGAAGAGAAAGGGAAAGAGAAATAGAAAAGAAAAAAAACAAATCAAGAAGACATTATATATATATTAATAAAGTGTTAAAGCCACCTTTTCCTTGCCAGATGGAACAGCTCAAGAAGACGTTCTTGCCTTATTTTATTAAGGCATATGAACCCTTTCGAAAGGTAATAAACTGACTTTAAATAAGTACTTCAGGGGAAACCATATTCATTGATCTTTATCCGACTTATCTCATGAAACTCTCTTCTGTCATATTTCTTATTATCCTTAAACTCTCTTCTGTCACATTTCTTAACGGTTCTTAATTTTGTAGGTTGTACACCAAGAGGGCCTACACAGGAGAGGGCTTGGGAAGACAATCGAAAGAATTCTGAGGTTTTTCCAATCGTCTAAGGAGAAAATCATTGCTTCTTACACCAAGGAGAAGTTTATTCGATGTTTGCAATATATTGAGCAAAGTGGGAACACAATTAAGAGCAATATCAATGTTGTTAATAAGCCGTCGTCCCTACATGATGGCCAGCCTGGCCTTAGTGGATCTCGCATAAACCATCCAGTTCAGCAAAGTGGTGATAATGTGAAACTCCATTGTCAATCAGTGATCCGAACTACAACTGGCTCTGGTAGTTCTTCCGTAGCACCACAAGAAATAGGATCCATAAGGTCAAAATTACATCCCCAATGGATTCATGGTTCAGGAAACACTCCATTTACTTATAGATCAGGAATCTCTTTGAATCCTCATTTGAACTCAAACTTTTCCCATGTTGCTGAGCGACCTCGTCCAACAAATCCTTGTACTTATCCATTGCATGGAAGAGCTTCTCCACCTCCATCTTCCTCTATAGTTGGACTTGAAAAAATCTCTCCAAATGTTACTTACCATTCAAGTTCGAACTTTCATTTCCGTCCACATTGTAACCCATATCAGTTACTTCATTCCAAAGCAGAAATGATCGCTGAACCTACTAGTCTTGGAATCAATGGGCAACTGTCAACGTATCAGGCTCATAATCGCTTACTTAAAGCGGTAAGTCTATGCAAGAATATCAAATGAAATGCATATTTCAAATATCAACTACTGACAGGAATGTGTTTGCATTTGTCCAATTATTTCAAATATCAACTGACAGAATGTGTTTGCATTTGTCCAATCTATTGGATTTCTCTAGGATGATAATTTCATTCTTCTTTTCCTTTGGCACTTACAATTAGTTTATTATGAGACATAACAAAATATGCAGGTTGGGTCATCATCAGAGGAAGCACTCAGAGCAGCTGTGTCGGGAATAACTTCAGTTGGGTATATGGAAGACGCGATAATAGATCCCCAGTGTCGTGCAAAGGTAACAAATTTAAGGTTGATAGACGGATTTGGTTCCTCAAATAATATGAAGCGCAAAATAAACGCAATGGCCTTAAACAACATACCATCGCCTAGCAGTGAGATTCTTGGATCTGAAGAAACAGTTACATCAAGAACAAAGAAACTCAAGAAACTGGTATGGCATCATCTTTATCTAGTCTCGTTGGAACTGTATTTCTATGCATTTTTCTTACAAATTTTCATTTCTCTACTGACAATCATGCAGTCTGATTCTTCCCTCTTAGAAGAAATGAGAAACATAAACAAGCAGTTCATCGAAACAGTCTTGGAACTAGATTTGGACGAGAACCTCAATCAACGATTAGCCAATGCGGGTACGGTTCTTCGATACTCCTATAGTGCTGTAAGTGATGGTACAAATTCTGTGAAACTGCCCGTGCTTACTATGAAGCTGCTTGTACCTCTTGATTATCCTGAAGACTATCCCGTATTTCTAAGCAAATTCGACTTGTCCTCCAGGTAACGACTGACTCTTGTATATGAGTAGTTTTCTTGTACCTTTCACTGAAGTTCTTTCATACATTTCTATAGCAACGTGGACGAAGAAAGTAGAAACCTGTCGAACGGTGCAATATCGATGTTACGTGCATTCCTTCGCACTGCTCCGGAATGTGTGTCTCTTGAAGATTATGCAAGGGCATGGGATGAATGTGCTCGCTCTGTGCTATCTGAGTATGTTCGACGTGCGGGTGGGGGAAGCTTCAGTGCTCGATATGGGTCTTGGGAGGACTCTGTTGTCGCCGCCTAACCCCTCCCTTTATT

Coding sequence (CDS)

ATGGAACAGCTCAAGAAGACGTTCTTGCCTTATTTTATTAAGGCATATGAACCCTTTCGAAAGGTTGTACACCAAGAGGGCCTACACAGGAGAGGGCTTGGGAAGACAATCGAAAGAATTCTGAGGTTTTTCCAATCGTCTAAGGAGAAAATCATTGCTTCTTACACCAAGGAGAAGTTTATTCGATGTTTGCAATATATTGAGCAAAGTGGGAACACAATTAAGAGCAATATCAATGTTGTTAATAAGCCGTCGTCCCTACATGATGGCCAGCCTGGCCTTAGTGGATCTCGCATAAACCATCCAGTTCAGCAAAGTGGTGATAATGTGAAACTCCATTGTCAATCAGTGATCCGAACTACAACTGGCTCTGGTAGTTCTTCCGTAGCACCACAAGAAATAGGATCCATAAGGTCAAAATTACATCCCCAATGGATTCATGGTTCAGGAAACACTCCATTTACTTATAGATCAGGAATCTCTTTGAATCCTCATTTGAACTCAAACTTTTCCCATGTTGCTGAGCGACCTCGTCCAACAAATCCTTGTACTTATCCATTGCATGGAAGAGCTTCTCCACCTCCATCTTCCTCTATAGTTGGACTTGAAAAAATCTCTCCAAATGTTACTTACCATTCAAGTTCGAACTTTCATTTCCGTCCACATTGTAACCCATATCAGTTACTTCATTCCAAAGCAGAAATGATCGCTGAACCTACTAGTCTTGGAATCAATGGGCAACTGTCAACGTATCAGGCTCATAATCGCTTACTTAAAGCGGTTGGGTCATCATCAGAGGAAGCACTCAGAGCAGCTGTGTCGGGAATAACTTCAGTTGGGTATATGGAAGACGCGATAATAGATCCCCAGTGTCGTGCAAAGGTAACAAATTTAAGGTTGATAGACGGATTTGGTTCCTCAAATAATATGAAGCGCAAAATAAACGCAATGGCCTTAAACAACATACCATCGCCTAGCAGTGAGATTCTTGGATCTGAAGAAACAGTTACATCAAGAACAAAGAAACTCAAGAAACTGTCTGATTCTTCCCTCTTAGAAGAAATGAGAAACATAAACAAGCAGTTCATCGAAACAGTCTTGGAACTAGATTTGGACGAGAACCTCAATCAACGATTAGCCAATGCGGGTACGGTTCTTCGATACTCCTATAGTGCTGTAAGTGATGGTACAAATTCTGTGAAACTGCCCGTGCTTACTATGAAGCTGCTTGTACCTCTTGATTATCCTGAAGACTATCCCGTATTTCTAAGCAAATTCGACTTGTCCTCCAGCAACGTGGACGAAGAAAGTAGAAACCTGTCGAACGGTGCAATATCGATGTTACGTGCATTCCTTCGCACTGCTCCGGAATGTGTGTCTCTTGAAGATTATGCAAGGGCATGGGATGAATGTGCTCGCTCTGTGCTATCTGAGTATGTTCGACGTGCGGGTGGGGGAAGCTTCAGTGCTCGATATGGGTCTTGGGAGGACTCTGTTGTCGCCGCCTAA

Protein sequence

MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKFIRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRTTTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPTNPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPTSLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYPVFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRAGGGSFSARYGSWEDSVVAA
Homology
BLAST of Cucsat.G13206.T2 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.3e-29
Identity = 95/290 (32.76%), Postives = 149/290 (51.38%), Query Frame = 0

Query: 256  RLLKAVGSSSEEALRAAVSGITSVGYM------------------EDAIIDPQCRAKVTN 315
            RL++AV S S +AL +AVS I SV  M                  ED +   +CR +  N
Sbjct: 1049 RLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDLVAMTKCRLQARN 1108

Query: 316  LRLIDGFGSSNNMKRKINAMAL-----------NNIPSPSSEILGSEETVTSRTKKLKKL 375
                +G  ++  MKR   AM L           N      SE    E T TS  KK +  
Sbjct: 1109 FMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKARTE 1168

Query: 376  SDSSLLEEMRNINKQFIETVLELDLDENLN-------QRLANAGTVLRYSYSAVSDG--- 435
            ++ +LLEE++ IN++ I+TV+E+  DE+           +   GT +R+S+ AVS     
Sbjct: 1169 TEHALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTTVRFSFIAVSLSPAL 1228

Query: 436  ----TNSVKLPVLTMKLLVPLDYPEDYPVFLSKFDLSSSNVDEESRNLSNGAISMLRAFL 495
                +++   P+  ++LLVP  YP   P  L K  + +S   +E+ +LS+ A++     L
Sbjct: 1229 KAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETS---KENEDLSSKAMARFNILL 1288

Query: 496  RTAPECVSLEDYARAWDECARSVLSEYVRRAGGGSFSARYGSWEDSVVAA 503
            R+  + +SL+D A+ WD CAR+V+ EY ++ GGG+FS++YG+WE  V A+
Sbjct: 1289 RSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYVAAS 1335

BLAST of Cucsat.G13206.T2 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-13
Identity = 75/282 (26.60%), Postives = 131/282 (46.45%), Query Frame = 0

Query: 255 NRLLKAVGSSSEEALRAAVSGITSVGYMEDAI-------------IDPQCRAKVTNLRLI 314
           +RL+KA  ++S ++L  +VS I+SV  M D I             +      +  N    
Sbjct: 661 DRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDLSERTRNFTTH 720

Query: 315 DGFGSSNNMKRKINAMALNNIPSPSSEILGSEE---------TVTSRTKKLKKLSDS-SL 374
           +    S  MKR IN +     P  SS+I   E+         + TS   K+  ++   +L
Sbjct: 721 EETNLSKRMKRSINIVP----PDMSSQIDSYEQLSSLESEVVSTTSSGLKVNNIAPGYAL 780

Query: 375 LEEMRNINKQFIETVLELDLDENLNQRLANAGTVLRYSYSAVS------DGTNSVKL--- 434
           L+E++  N + +ETV+E+  +++L       GT++  +Y+ V+      D   S K+   
Sbjct: 781 LQEIKETNGRLVETVVEICDEDSL-------GTIVTCTYAPVALSATFKDHYKSGKIIFY 840

Query: 435 --------PVLTMKLLVPLDYPEDYPVFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRT 494
                    +  ++LL P+DYP   P+ L +    +S    +  +LS    S     ++ 
Sbjct: 841 VSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTS--VHKYEDLSARTRSRFSLSMKE 900

Query: 495 APECVSLEDYARAWDECARSVLSEYVRRAGGGSFSARYGSWE 497
             E    +  A+ W++CAR+ + EY  R GGG+FS++YG+WE
Sbjct: 901 FSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWE 929

BLAST of Cucsat.G13206.T2 vs. NCBI nr
Match: XP_011652173.1 (uncharacterized protein LOC105434992 [Cucumis sativus] >KAE8651172.1 hypothetical protein Csa_002482 [Cucumis sativus])

HSP 1 Score: 989 bits (2558), Expect = 0.0
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF
Sbjct: 220 MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 279

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
           IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT
Sbjct: 280 IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 339

Query: 121 TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT 180
           TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT
Sbjct: 340 TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT 399

Query: 181 NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT 240
           NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT
Sbjct: 400 NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT 459

Query: 241 SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL 300
           SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL
Sbjct: 460 SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL 519

Query: 301 IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK 360
           IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK
Sbjct: 520 IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK 579

Query: 361 QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP 420
           QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP
Sbjct: 580 QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP 639

Query: 421 VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV 480
           VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV
Sbjct: 640 VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV 699

Query: 481 RRAGGGSFSARYGSWEDSVVAA 502
           RRAGGGSFSARYGSWEDSVVAA
Sbjct: 700 RRAGGGSFSARYGSWEDSVVAA 721

BLAST of Cucsat.G13206.T2 vs. NCBI nr
Match: XP_008443239.1 (PREDICTED: uncharacterized protein LOC103486878 [Cucumis melo] >XP_008443241.1 PREDICTED: uncharacterized protein LOC103486878 [Cucumis melo] >XP_008443242.1 PREDICTED: uncharacterized protein LOC103486878 [Cucumis melo] >XP_016899629.1 PREDICTED: uncharacterized protein LOC103486878 [Cucumis melo] >ADN33883.1 hypothetical protein [Cucumis melo subsp. melo] >KAA0053897.1 putative tartrate dehydrogenase/decarboxylase ttuC [Cucumis melo var. makuwa] >TYK25509.1 putative tartrate dehydrogenase/decarboxylase ttuC [Cucumis melo var. makuwa])

HSP 1 Score: 755 bits (1949), Expect = 9.57e-267
Identity = 411/555 (74.05%), Postives = 440/555 (79.28%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLP  IKAYEP+RKV H +  H+  L KT+ERIL FF S KEKIIASYTKE+F
Sbjct: 216 MEQLKKTFLPLIIKAYEPYRKV-HPDAQHKNRLMKTLERILTFFHSPKEKIIASYTKERF 275

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCL+YIEQ GNTIK N NV NK SSLH GQPGLSGSRINHP+QQS DNVKL CQSVIR 
Sbjct: 276 YRCLKYIEQFGNTIKCNTNVANKQSSLHGGQPGLSGSRINHPLQQS-DNVKLPCQSVIRA 335

Query: 121 TTGSGSSS--VAPQEIGSIRS---------------------KLHPQWIHGSGNTPFTYR 180
           TTGS  SS  +APQE GS+RS                     K+HPQWIH SGNTP TYR
Sbjct: 336 TTGSSGSSSPIAPQEKGSVRSETDYIQKNLLQNRQHYKSIKSKVHPQWIHASGNTPATYR 395

Query: 181 SGISLNPHLNSNFSH----------VAERPRPTNPCTYPLHGRASPPPSSSIVGLEKISP 240
           SG+SLN HLNSNFSH          VAERPRPT PCT PL+G ASP PSS IVGLEK SP
Sbjct: 396 SGMSLNHHLNSNFSHQIHDASQLCHVAERPRPTKPCTSPLYGIASPAPSSPIVGLEKTSP 455

Query: 241 NVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQLSTY 300
           NVTYHS  NF    HCNPYQLLHSK E                 +AEPTS GINGQ STY
Sbjct: 456 NVTYHSGLNFQSPQHCNPYQLLHSKTETQVPSQKIRSSSAMTSPVAEPTSPGINGQFSTY 515

Query: 301 QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSSNNMK 360
           QA++RLLKAVGSSS  ALRAAVSGITSVGYMEDAIIDP+C A VTNLRL++G GSSNNMK
Sbjct: 516 QAYSRLLKAVGSSSRAALRAAVSGITSVGYMEDAIIDPRCHAMVTNLRLLNGCGSSNNMK 575

Query: 361 RKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVLELDL 420
           RKINAMALNNIPSP S+I GSEETVTSRTKKLKK +DSSLLEE+RNINKQFIETVLELD+
Sbjct: 576 RKINAMALNNIPSPRSDIPGSEETVTSRTKKLKKHTDSSLLEEIRNINKQFIETVLELDV 635

Query: 421 DENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFLSKFD 480
           DENLN+RLANAGTVLR SYSAV DGTNS    VKLPVLTMKLLVPLDYPEDYPVFLSKFD
Sbjct: 636 DENLNRRLANAGTVLRCSYSAVIDGTNSEAYPVKLPVLTMKLLVPLDYPEDYPVFLSKFD 695

Query: 481 LSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRAGGGS 502
             SSNVDEE  NLSN A+SMLRAFLRTAPECVSLE+YAR WDECARSV+S+YV+RAGGGS
Sbjct: 696 SGSSNVDEECSNLSNEAMSMLRAFLRTAPECVSLEEYARVWDECARSVVSDYVQRAGGGS 755

BLAST of Cucsat.G13206.T2 vs. NCBI nr
Match: XP_038903081.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Benincasa hispida])

HSP 1 Score: 567 bits (1462), Expect = 5.67e-193
Identity = 331/559 (59.21%), Postives = 389/559 (69.59%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           ME+LKKT+LP   KA E   +VV  E + ++     +  +++F Q  ++KII +Y KEKF
Sbjct: 209 MERLKKTYLPLLEKACELSLQVVQAEQIQQQK-HDPLRGMMKFLQLPRDKIILTYDKEKF 268

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCLQ IE+ G  IKS  N+ NK   LH GQPG  GSR+N PVQQS ++VKLH Q VIR 
Sbjct: 269 YRCLQTIEKVGKAIKSKFNLGNKQQPLHGGQPGPGGSRLN-PVQQS-NSVKLHRQPVIRA 328

Query: 121 TTG--SGSSSVAPQEIGSIRSK---------------------LHPQWI----HGSGNTP 180
           TTG   G+S +A  E GS+RS+                        QW+    + +GN P
Sbjct: 329 TTGFPDGTSPIASPEKGSVRSETDCIQINLLQNRQHFENIESEFRSQWLQPKQNATGNIP 388

Query: 181 FTYRSGISLNP---------HLNSNFSHVAERPRPTNPCTYPLHGRASPPPSSSIVGLEK 240
             YRSG+SLN          H  S  S  AERP PTNPC   LHGRASP PSSSIV L+K
Sbjct: 389 AIYRSGMSLNHYCSNVSPQIHEASQLSQFAERPLPTNPCGSSLHGRASPAPSSSIVRLDK 448

Query: 241 ISPNVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQL 300
            SPNV+Y SSSNF F  +CNP + LH KAE+                 A PTSLG NGQL
Sbjct: 449 SSPNVSYLSSSNFQFPQNCNPLEFLHPKAEIEVQSQKIRSSSAMTSPFATPTSLGSNGQL 508

Query: 301 STY-QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSS 360
            T  QAHNRLLKAV S S EAL  AVSGI+SVGY +DA+IDP C AKVT++RL DG GSS
Sbjct: 509 PTATQAHNRLLKAVESLSNEALTIAVSGISSVGYSDDAMIDPWCHAKVTDVRLQDGSGSS 568

Query: 361 NNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVL 420
           NNMKRKINA ALNNIPSP S+I GSE TVTSR KKLKKLSD SLLEE+RNINKQF+ETVL
Sbjct: 569 NNMKRKINATALNNIPSPCSDITGSEPTVTSRRKKLKKLSDYSLLEELRNINKQFVETVL 628

Query: 421 ELDLDENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFL 480
           ELDLDE+LN++LANAGTVLR SYSA ++  NS    VKLPVL++KLLVPLDYPEDYPVFL
Sbjct: 629 ELDLDESLNRKLANAGTVLRCSYSAATECKNSEACPVKLPVLSVKLLVPLDYPEDYPVFL 688

Query: 481 SKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRA 502
           SKF+ +S NVD+E R+LSN A  MLRAFLRTAP+C+SL +YAR WDECARSV+SEY +RA
Sbjct: 689 SKFNTNSGNVDKEFRDLSNEATLMLRAFLRTAPDCLSLLEYARVWDECARSVVSEYAQRA 748

BLAST of Cucsat.G13206.T2 vs. NCBI nr
Match: XP_038903080.1 (probable mediator of RNA polymerase II transcription subunit 15c isoform X1 [Benincasa hispida])

HSP 1 Score: 567 bits (1462), Expect = 1.45e-192
Identity = 331/559 (59.21%), Postives = 389/559 (69.59%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           ME+LKKT+LP   KA E   +VV  E + ++     +  +++F Q  ++KII +Y KEKF
Sbjct: 239 MERLKKTYLPLLEKACELSLQVVQAEQIQQQK-HDPLRGMMKFLQLPRDKIILTYDKEKF 298

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCLQ IE+ G  IKS  N+ NK   LH GQPG  GSR+N PVQQS ++VKLH Q VIR 
Sbjct: 299 YRCLQTIEKVGKAIKSKFNLGNKQQPLHGGQPGPGGSRLN-PVQQS-NSVKLHRQPVIRA 358

Query: 121 TTG--SGSSSVAPQEIGSIRSK---------------------LHPQWI----HGSGNTP 180
           TTG   G+S +A  E GS+RS+                        QW+    + +GN P
Sbjct: 359 TTGFPDGTSPIASPEKGSVRSETDCIQINLLQNRQHFENIESEFRSQWLQPKQNATGNIP 418

Query: 181 FTYRSGISLNP---------HLNSNFSHVAERPRPTNPCTYPLHGRASPPPSSSIVGLEK 240
             YRSG+SLN          H  S  S  AERP PTNPC   LHGRASP PSSSIV L+K
Sbjct: 419 AIYRSGMSLNHYCSNVSPQIHEASQLSQFAERPLPTNPCGSSLHGRASPAPSSSIVRLDK 478

Query: 241 ISPNVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQL 300
            SPNV+Y SSSNF F  +CNP + LH KAE+                 A PTSLG NGQL
Sbjct: 479 SSPNVSYLSSSNFQFPQNCNPLEFLHPKAEIEVQSQKIRSSSAMTSPFATPTSLGSNGQL 538

Query: 301 STY-QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSS 360
            T  QAHNRLLKAV S S EAL  AVSGI+SVGY +DA+IDP C AKVT++RL DG GSS
Sbjct: 539 PTATQAHNRLLKAVESLSNEALTIAVSGISSVGYSDDAMIDPWCHAKVTDVRLQDGSGSS 598

Query: 361 NNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVL 420
           NNMKRKINA ALNNIPSP S+I GSE TVTSR KKLKKLSD SLLEE+RNINKQF+ETVL
Sbjct: 599 NNMKRKINATALNNIPSPCSDITGSEPTVTSRRKKLKKLSDYSLLEELRNINKQFVETVL 658

Query: 421 ELDLDENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFL 480
           ELDLDE+LN++LANAGTVLR SYSA ++  NS    VKLPVL++KLLVPLDYPEDYPVFL
Sbjct: 659 ELDLDESLNRKLANAGTVLRCSYSAATECKNSEACPVKLPVLSVKLLVPLDYPEDYPVFL 718

Query: 481 SKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRA 502
           SKF+ +S NVD+E R+LSN A  MLRAFLRTAP+C+SL +YAR WDECARSV+SEY +RA
Sbjct: 719 SKFNTNSGNVDKEFRDLSNEATLMLRAFLRTAPDCLSLLEYARVWDECARSVVSEYAQRA 778

BLAST of Cucsat.G13206.T2 vs. NCBI nr
Match: XP_022147806.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Momordica charantia])

HSP 1 Score: 414 bits (1063), Expect = 1.36e-132
Identity = 274/576 (47.57%), Postives = 349/576 (60.59%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHR-----RGLGKTIERILRFFQSSKEKIIASY 60
           M+ LK T LP   ++YE   K+  + G        +     ++++  F +  ++KI   +
Sbjct: 236 MQGLKMTCLPLLKESYERAAKLCLEVGQTEQIQKCKNHMSLMQKMTNFLELPRDKI-TYF 295

Query: 61  TKEKFIRCLQYIEQSGNTIKS-NINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHC 120
           TKEKF + ++ IE+     ++ N  +VNK   LH GQPG+S S IN PVQ+S DN   H 
Sbjct: 296 TKEKFYQSMKSIEKFAKAHENRNTFLVNKQQPLH-GQPGISQSHIN-PVQRS-DNANPHF 355

Query: 121 QSVIRTTTGSGSSSV--APQEIGS---------------------IRSKLHPQWIH---- 180
           Q +    TGS  SS    P EIGS                     I+ +    WI     
Sbjct: 356 QPLNLAATGSSDSSSPRTPSEIGSSRPEANRIQKNLLQKRQQPEIIKQEFQSSWIQQMQK 415

Query: 181 GSGNTPFTYRSGISLNPHLNSN-----------FSHVAERPRPTNPCT--YPLHGRASPP 240
            + +     RSG+SL  HLNS             S +AER    +PC+  Y   GRASP 
Sbjct: 416 STESIQAINRSGVSLQNHLNSKHSPQSHEEASQLSKIAERALSKDPCSSVYGRDGRASPT 475

Query: 241 PSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAE 300
           PSSS VGL K S NV+  SS NF +    N   LL+SK ++                 AE
Sbjct: 476 PSSSTVGLGKSSSNVSCLSSLNFQYPETRNSVNLLNSKTKIQVKSHEIRSSGISTSQFAE 535

Query: 301 PTSLGINGQLSTY-QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDP-----QCR 360
           PTSLG +  LST  Q  NRLLKAV S S++ALR A+SGI+SVG M D + +P     +C 
Sbjct: 536 PTSLGSSQHLSTATQPLNRLLKAVDSMSDQALRTAISGISSVGNMCDTVTEPSVFGTRCH 595

Query: 361 AKVTNLRLIDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLL 420
            K  +L L DGFGSSNNMKRKI A+ LN++PSP S+  GSE TVTSR+KKLKKL+D++LL
Sbjct: 596 QKAAHLSLQDGFGSSNNMKRKICAITLNDMPSPCSDNDGSELTVTSRSKKLKKLTDNALL 655

Query: 421 EEMRNINKQFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTN-------SVKLPVL 480
           EEMRNIN++ +ETVLELD  +N+N+R ANAGTV+R +YSAVSD  N       ++KLPVL
Sbjct: 656 EEMRNINQRLVETVLELDSAQNVNRRFANAGTVVRCTYSAVSDRNNLMFHFANTLKLPVL 715

Query: 481 TMKLLVPLDYPEDYPVFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYA 501
           ++KLLVPLDYPEDYPVFLSKF+    N DEE R+LS  A SMLRAFLRTAPE +SL +YA
Sbjct: 716 SVKLLVPLDYPEDYPVFLSKFNTDCGNEDEECRDLSRKATSMLRAFLRTAPERLSLGEYA 775

BLAST of Cucsat.G13206.T2 vs. ExPASy TrEMBL
Match: A0A0A0LET7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G819860 PE=4 SV=1)

HSP 1 Score: 989 bits (2558), Expect = 0.0
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF
Sbjct: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
           IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT
Sbjct: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120

Query: 121 TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT 180
           TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT
Sbjct: 121 TTGSGSSSVAPQEIGSIRSKLHPQWIHGSGNTPFTYRSGISLNPHLNSNFSHVAERPRPT 180

Query: 181 NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT 240
           NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT
Sbjct: 181 NPCTYPLHGRASPPPSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEMIAEPT 240

Query: 241 SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL 300
           SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL
Sbjct: 241 SLGINGQLSTYQAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRL 300

Query: 301 IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK 360
           IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK
Sbjct: 301 IDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINK 360

Query: 361 QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP 420
           QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP
Sbjct: 361 QFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTNSVKLPVLTMKLLVPLDYPEDYP 420

Query: 421 VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV 480
           VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV
Sbjct: 421 VFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYV 480

Query: 481 RRAGGGSFSARYGSWEDSVVAA 502
           RRAGGGSFSARYGSWEDSVVAA
Sbjct: 481 RRAGGGSFSARYGSWEDSVVAA 502

BLAST of Cucsat.G13206.T2 vs. ExPASy TrEMBL
Match: A0A1S4DUH4 (uncharacterized protein LOC103486878 OS=Cucumis melo OX=3656 GN=LOC103486878 PE=4 SV=1)

HSP 1 Score: 755 bits (1949), Expect = 4.63e-267
Identity = 411/555 (74.05%), Postives = 440/555 (79.28%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLP  IKAYEP+RKV H +  H+  L KT+ERIL FF S KEKIIASYTKE+F
Sbjct: 216 MEQLKKTFLPLIIKAYEPYRKV-HPDAQHKNRLMKTLERILTFFHSPKEKIIASYTKERF 275

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCL+YIEQ GNTIK N NV NK SSLH GQPGLSGSRINHP+QQS DNVKL CQSVIR 
Sbjct: 276 YRCLKYIEQFGNTIKCNTNVANKQSSLHGGQPGLSGSRINHPLQQS-DNVKLPCQSVIRA 335

Query: 121 TTGSGSSS--VAPQEIGSIRS---------------------KLHPQWIHGSGNTPFTYR 180
           TTGS  SS  +APQE GS+RS                     K+HPQWIH SGNTP TYR
Sbjct: 336 TTGSSGSSSPIAPQEKGSVRSETDYIQKNLLQNRQHYKSIKSKVHPQWIHASGNTPATYR 395

Query: 181 SGISLNPHLNSNFSH----------VAERPRPTNPCTYPLHGRASPPPSSSIVGLEKISP 240
           SG+SLN HLNSNFSH          VAERPRPT PCT PL+G ASP PSS IVGLEK SP
Sbjct: 396 SGMSLNHHLNSNFSHQIHDASQLCHVAERPRPTKPCTSPLYGIASPAPSSPIVGLEKTSP 455

Query: 241 NVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQLSTY 300
           NVTYHS  NF    HCNPYQLLHSK E                 +AEPTS GINGQ STY
Sbjct: 456 NVTYHSGLNFQSPQHCNPYQLLHSKTETQVPSQKIRSSSAMTSPVAEPTSPGINGQFSTY 515

Query: 301 QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSSNNMK 360
           QA++RLLKAVGSSS  ALRAAVSGITSVGYMEDAIIDP+C A VTNLRL++G GSSNNMK
Sbjct: 516 QAYSRLLKAVGSSSRAALRAAVSGITSVGYMEDAIIDPRCHAMVTNLRLLNGCGSSNNMK 575

Query: 361 RKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVLELDL 420
           RKINAMALNNIPSP S+I GSEETVTSRTKKLKK +DSSLLEE+RNINKQFIETVLELD+
Sbjct: 576 RKINAMALNNIPSPRSDIPGSEETVTSRTKKLKKHTDSSLLEEIRNINKQFIETVLELDV 635

Query: 421 DENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFLSKFD 480
           DENLN+RLANAGTVLR SYSAV DGTNS    VKLPVLTMKLLVPLDYPEDYPVFLSKFD
Sbjct: 636 DENLNRRLANAGTVLRCSYSAVIDGTNSEAYPVKLPVLTMKLLVPLDYPEDYPVFLSKFD 695

Query: 481 LSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRAGGGS 502
             SSNVDEE  NLSN A+SMLRAFLRTAPECVSLE+YAR WDECARSV+S+YV+RAGGGS
Sbjct: 696 SGSSNVDEECSNLSNEAMSMLRAFLRTAPECVSLEEYARVWDECARSVVSDYVQRAGGGS 755

BLAST of Cucsat.G13206.T2 vs. ExPASy TrEMBL
Match: A0A5A7UH89 (Putative tartrate dehydrogenase/decarboxylase ttuC OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006580 PE=4 SV=1)

HSP 1 Score: 755 bits (1949), Expect = 4.63e-267
Identity = 411/555 (74.05%), Postives = 440/555 (79.28%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLP  IKAYEP+RKV H +  H+  L KT+ERIL FF S KEKIIASYTKE+F
Sbjct: 216 MEQLKKTFLPLIIKAYEPYRKV-HPDAQHKNRLMKTLERILTFFHSPKEKIIASYTKERF 275

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCL+YIEQ GNTIK N NV NK SSLH GQPGLSGSRINHP+QQS DNVKL CQSVIR 
Sbjct: 276 YRCLKYIEQFGNTIKCNTNVANKQSSLHGGQPGLSGSRINHPLQQS-DNVKLPCQSVIRA 335

Query: 121 TTGSGSSS--VAPQEIGSIRS---------------------KLHPQWIHGSGNTPFTYR 180
           TTGS  SS  +APQE GS+RS                     K+HPQWIH SGNTP TYR
Sbjct: 336 TTGSSGSSSPIAPQEKGSVRSETDYIQKNLLQNRQHYKSIKSKVHPQWIHASGNTPATYR 395

Query: 181 SGISLNPHLNSNFSH----------VAERPRPTNPCTYPLHGRASPPPSSSIVGLEKISP 240
           SG+SLN HLNSNFSH          VAERPRPT PCT PL+G ASP PSS IVGLEK SP
Sbjct: 396 SGMSLNHHLNSNFSHQIHDASQLCHVAERPRPTKPCTSPLYGIASPAPSSPIVGLEKTSP 455

Query: 241 NVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQLSTY 300
           NVTYHS  NF    HCNPYQLLHSK E                 +AEPTS GINGQ STY
Sbjct: 456 NVTYHSGLNFQSPQHCNPYQLLHSKTETQVPSQKIRSSSAMTSPVAEPTSPGINGQFSTY 515

Query: 301 QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSSNNMK 360
           QA++RLLKAVGSSS  ALRAAVSGITSVGYMEDAIIDP+C A VTNLRL++G GSSNNMK
Sbjct: 516 QAYSRLLKAVGSSSRAALRAAVSGITSVGYMEDAIIDPRCHAMVTNLRLLNGCGSSNNMK 575

Query: 361 RKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVLELDL 420
           RKINAMALNNIPSP S+I GSEETVTSRTKKLKK +DSSLLEE+RNINKQFIETVLELD+
Sbjct: 576 RKINAMALNNIPSPRSDIPGSEETVTSRTKKLKKHTDSSLLEEIRNINKQFIETVLELDV 635

Query: 421 DENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFLSKFD 480
           DENLN+RLANAGTVLR SYSAV DGTNS    VKLPVLTMKLLVPLDYPEDYPVFLSKFD
Sbjct: 636 DENLNRRLANAGTVLRCSYSAVIDGTNSEAYPVKLPVLTMKLLVPLDYPEDYPVFLSKFD 695

Query: 481 LSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRAGGGS 502
             SSNVDEE  NLSN A+SMLRAFLRTAPECVSLE+YAR WDECARSV+S+YV+RAGGGS
Sbjct: 696 SGSSNVDEECSNLSNEAMSMLRAFLRTAPECVSLEEYARVWDECARSVVSDYVQRAGGGS 755

BLAST of Cucsat.G13206.T2 vs. ExPASy TrEMBL
Match: E5GBP1 (KIX_2 domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 755 bits (1949), Expect = 4.63e-267
Identity = 411/555 (74.05%), Postives = 440/555 (79.28%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHRRGLGKTIERILRFFQSSKEKIIASYTKEKF 60
           MEQLKKTFLP  IKAYEP+RKV H +  H+  L KT+ERIL FF S KEKIIASYTKE+F
Sbjct: 216 MEQLKKTFLPLIIKAYEPYRKV-HPDAQHKNRLMKTLERILTFFHSPKEKIIASYTKERF 275

Query: 61  IRCLQYIEQSGNTIKSNINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHCQSVIRT 120
            RCL+YIEQ GNTIK N NV NK SSLH GQPGLSGSRINHP+QQS DNVKL CQSVIR 
Sbjct: 276 YRCLKYIEQFGNTIKCNTNVANKQSSLHGGQPGLSGSRINHPLQQS-DNVKLPCQSVIRA 335

Query: 121 TTGSGSSS--VAPQEIGSIRS---------------------KLHPQWIHGSGNTPFTYR 180
           TTGS  SS  +APQE GS+RS                     K+HPQWIH SGNTP TYR
Sbjct: 336 TTGSSGSSSPIAPQEKGSVRSETDYIQKNLLQNRQHYKSIKSKVHPQWIHASGNTPATYR 395

Query: 181 SGISLNPHLNSNFSH----------VAERPRPTNPCTYPLHGRASPPPSSSIVGLEKISP 240
           SG+SLN HLNSNFSH          VAERPRPT PCT PL+G ASP PSS IVGLEK SP
Sbjct: 396 SGMSLNHHLNSNFSHQIHDASQLCHVAERPRPTKPCTSPLYGIASPAPSSPIVGLEKTSP 455

Query: 241 NVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAEPTSLGINGQLSTY 300
           NVTYHS  NF    HCNPYQLLHSK E                 +AEPTS GINGQ STY
Sbjct: 456 NVTYHSGLNFQSPQHCNPYQLLHSKTETQVPSQKIRSSSAMTSPVAEPTSPGINGQFSTY 515

Query: 301 QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDPQCRAKVTNLRLIDGFGSSNNMK 360
           QA++RLLKAVGSSS  ALRAAVSGITSVGYMEDAIIDP+C A VTNLRL++G GSSNNMK
Sbjct: 516 QAYSRLLKAVGSSSRAALRAAVSGITSVGYMEDAIIDPRCHAMVTNLRLLNGCGSSNNMK 575

Query: 361 RKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLLEEMRNINKQFIETVLELDL 420
           RKINAMALNNIPSP S+I GSEETVTSRTKKLKK +DSSLLEE+RNINKQFIETVLELD+
Sbjct: 576 RKINAMALNNIPSPRSDIPGSEETVTSRTKKLKKHTDSSLLEEIRNINKQFIETVLELDV 635

Query: 421 DENLNQRLANAGTVLRYSYSAVSDGTNS----VKLPVLTMKLLVPLDYPEDYPVFLSKFD 480
           DENLN+RLANAGTVLR SYSAV DGTNS    VKLPVLTMKLLVPLDYPEDYPVFLSKFD
Sbjct: 636 DENLNRRLANAGTVLRCSYSAVIDGTNSEAYPVKLPVLTMKLLVPLDYPEDYPVFLSKFD 695

Query: 481 LSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYARAWDECARSVLSEYVRRAGGGS 502
             SSNVDEE  NLSN A+SMLRAFLRTAPECVSLE+YAR WDECARSV+S+YV+RAGGGS
Sbjct: 696 SGSSNVDEECSNLSNEAMSMLRAFLRTAPECVSLEEYARVWDECARSVVSDYVQRAGGGS 755

BLAST of Cucsat.G13206.T2 vs. ExPASy TrEMBL
Match: A0A6J1D3G7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016654 PE=4 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 6.59e-133
Identity = 274/576 (47.57%), Postives = 349/576 (60.59%), Query Frame = 0

Query: 1   MEQLKKTFLPYFIKAYEPFRKVVHQEGLHR-----RGLGKTIERILRFFQSSKEKIIASY 60
           M+ LK T LP   ++YE   K+  + G        +     ++++  F +  ++KI   +
Sbjct: 236 MQGLKMTCLPLLKESYERAAKLCLEVGQTEQIQKCKNHMSLMQKMTNFLELPRDKI-TYF 295

Query: 61  TKEKFIRCLQYIEQSGNTIKS-NINVVNKPSSLHDGQPGLSGSRINHPVQQSGDNVKLHC 120
           TKEKF + ++ IE+     ++ N  +VNK   LH GQPG+S S IN PVQ+S DN   H 
Sbjct: 296 TKEKFYQSMKSIEKFAKAHENRNTFLVNKQQPLH-GQPGISQSHIN-PVQRS-DNANPHF 355

Query: 121 QSVIRTTTGSGSSSV--APQEIGS---------------------IRSKLHPQWIH---- 180
           Q +    TGS  SS    P EIGS                     I+ +    WI     
Sbjct: 356 QPLNLAATGSSDSSSPRTPSEIGSSRPEANRIQKNLLQKRQQPEIIKQEFQSSWIQQMQK 415

Query: 181 GSGNTPFTYRSGISLNPHLNSN-----------FSHVAERPRPTNPCT--YPLHGRASPP 240
            + +     RSG+SL  HLNS             S +AER    +PC+  Y   GRASP 
Sbjct: 416 STESIQAINRSGVSLQNHLNSKHSPQSHEEASQLSKIAERALSKDPCSSVYGRDGRASPT 475

Query: 241 PSSSIVGLEKISPNVTYHSSSNFHFRPHCNPYQLLHSKAEM----------------IAE 300
           PSSS VGL K S NV+  SS NF +    N   LL+SK ++                 AE
Sbjct: 476 PSSSTVGLGKSSSNVSCLSSLNFQYPETRNSVNLLNSKTKIQVKSHEIRSSGISTSQFAE 535

Query: 301 PTSLGINGQLSTY-QAHNRLLKAVGSSSEEALRAAVSGITSVGYMEDAIIDP-----QCR 360
           PTSLG +  LST  Q  NRLLKAV S S++ALR A+SGI+SVG M D + +P     +C 
Sbjct: 536 PTSLGSSQHLSTATQPLNRLLKAVDSMSDQALRTAISGISSVGNMCDTVTEPSVFGTRCH 595

Query: 361 AKVTNLRLIDGFGSSNNMKRKINAMALNNIPSPSSEILGSEETVTSRTKKLKKLSDSSLL 420
            K  +L L DGFGSSNNMKRKI A+ LN++PSP S+  GSE TVTSR+KKLKKL+D++LL
Sbjct: 596 QKAAHLSLQDGFGSSNNMKRKICAITLNDMPSPCSDNDGSELTVTSRSKKLKKLTDNALL 655

Query: 421 EEMRNINKQFIETVLELDLDENLNQRLANAGTVLRYSYSAVSDGTN-------SVKLPVL 480
           EEMRNIN++ +ETVLELD  +N+N+R ANAGTV+R +YSAVSD  N       ++KLPVL
Sbjct: 656 EEMRNINQRLVETVLELDSAQNVNRRFANAGTVVRCTYSAVSDRNNLMFHFANTLKLPVL 715

Query: 481 TMKLLVPLDYPEDYPVFLSKFDLSSSNVDEESRNLSNGAISMLRAFLRTAPECVSLEDYA 501
           ++KLLVPLDYPEDYPVFLSKF+    N DEE R+LS  A SMLRAFLRTAPE +SL +YA
Sbjct: 716 SVKLLVPLDYPEDYPVFLSKFNTDCGNEDEECRDLSRKATSMLRAFLRTAPERLSLGEYA 775

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I1712.3e-2932.76Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana ... [more]
Q9SHV71.3e-1326.60Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis ... [more]
Match NameE-valueIdentityDescription
XP_011652173.10.0100.00uncharacterized protein LOC105434992 [Cucumis sativus] >KAE8651172.1 hypothetica... [more]
XP_008443239.19.57e-26774.05PREDICTED: uncharacterized protein LOC103486878 [Cucumis melo] >XP_008443241.1 P... [more]
XP_038903081.15.67e-19359.21mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Beninca... [more]
XP_038903080.11.45e-19259.21probable mediator of RNA polymerase II transcription subunit 15c isoform X1 [Ben... [more]
XP_022147806.11.36e-13247.57mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Momordi... [more]
Match NameE-valueIdentityDescription
A0A0A0LET70.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G819860 PE=4 SV=1[more]
A0A1S4DUH44.63e-26774.05uncharacterized protein LOC103486878 OS=Cucumis melo OX=3656 GN=LOC103486878 PE=... [more]
A0A5A7UH894.63e-26774.05Putative tartrate dehydrogenase/decarboxylase ttuC OS=Cucumis melo var. makuwa O... [more]
E5GBP14.63e-26774.05KIX_2 domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A6J1D3G76.59e-13347.57mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momor... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33137:SF4MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATEDcoord: 36..493
IPR044661Mediator of RNA polymerase II transcription subunit 15a/b/c-likePANTHERPTHR33137MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATEDcoord: 36..493

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G13206Cucsat.G13206gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G13206.T2.E1Cucsat.G13206.T2.E1exon
Cucsat.G13206.T2.E2Cucsat.G13206.T2.E2exon
Cucsat.G13206.T2.E3Cucsat.G13206.T2.E3exon
Cucsat.G13206.T2.E4Cucsat.G13206.T2.E4exon
Cucsat.G13206.T2.E5Cucsat.G13206.T2.E5exon
Cucsat.G13206.T2.E6Cucsat.G13206.T2.E6exon
Cucsat.G13206.T2.E7Cucsat.G13206.T2.E7exon
Cucsat.G13206.T2.E8Cucsat.G13206.T2.E8exon
Cucsat.G13206.T2.E9Cucsat.G13206.T2.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G13206.T2.C1Cucsat.G13206.T2.C1CDS
Cucsat.G13206.T2.C2Cucsat.G13206.T2.C2CDS
Cucsat.G13206.T2.C3Cucsat.G13206.T2.C3CDS
Cucsat.G13206.T2.C4Cucsat.G13206.T2.C4CDS
Cucsat.G13206.T2.C5Cucsat.G13206.T2.C5CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G13206.T2Cucsat.G13206.T2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0031490 chromatin DNA binding
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0003712 transcription coregulator activity