Cucsat.G12337.T5 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G12337.T5
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein kinase superfamily protein
Locationctg1837: 445220 .. 448936 (+)
RNA-Seq ExpressionCucsat.G12337.T5
SyntenyCucsat.G12337.T5
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTTTTCTCTTTTCCCTTTTCTCTGTTTTCAAGTTTGTGGAATCTTTTTTCTTCCTGGATTTTTTATTTTTGTGTCTCATTTTGTCTTAAGCTCTTTGGAAATCGAATCTGGGTTATCTTGGATTTAGGTTTCCTTTTTCTGTTTGGTGAAGACGGTGGAGCCTCTTCCGTTGGTGGTGGTTTTCCAGATTTCTGTCTTGGCCGATCGTATTATAAGGGTTGGGTTTTAGCTAGGGTTTCTTTTTTTCTATGATTCTGTTTTTGCTTTTTGGGTTTTGATTAGCTATTGAAGCTCTAGGAAACGGCATTAGGGTTGGATGTTAAGATACCTTATATGTTTGCCCCCTTTCGATTGAAAATTTTCAAGTCAAGATGTTATTTTTGCTGTTTGGGCTGTAGAACAACATGATACTTGTTGAATTTGCTCTCAGGAAGGTGTATTATTATTATTATTTTGTTCTTTTGGAGCAAGAGTTCTGTTGAAATATCTGTTAAATGTTGTGTAAAGACTTTTGTCTTTTTGGAGCTACTAGCTGAGCTTCATCCTTTGGTGTTTTTTCACTTTCCTTTTACATTATCCTTTGTTTTGCGACAGAAATTTCTTTGAATCCTTTTATATTTGTAGACTTGTTTATGCTCTATTATGTTATTTTCCACTATTTGCTTCTTCTTACATTTTTACCAACTGTTGCAGGTTGGTTACTTCTTTAGATAAGTGACTGTATTCAGAGTTAAGAACGTGAAGTTTGAAGCATATTTGCTGCTTATACATCTTGAAGCAAATGACATCAGTAAGCGTAGCTCCAGCTTCTATGATAAGGGATCATGGTGGGAGCACAATCGGTGTTGACCGGCTGCCTGATGAGATGAATGATATGAAAATTAGGGATGATAAGGTACCTAAGATGTTGTTTAGTTTTTTCCTTCCATGGCGCTAGTTAACAATACATTTCTTCTAACCTATAATTGGATGGAACAGGAAATTGAAGCCACCGTTGTTGATGGAAACGGTACTGAAACGGGCCATATAATTGTAACGACCATTGGTGGAAAGAATGGCCAACCAAAACAGGTGATTGTCAAAAAACTTATTCTCGAGACTACCGTTATTTAACTTCTTTCCTGACACCTCTTTTAAAACTTGTTTTAGACTATCAGTTACATGGCTGAGCGTGCTGTTGGACATGGTTCTTTTGGAGTTGTATTTCAGGTATGTTGCTCAGAGCTTAAAAGCTTTTCGATTAGTAGTGAGCATGCTAAGAGTAGAGGACATTTCATCCTTTTAATTTTTTAAGAAACAAGATATATTATCAATGTTATTTTGCAGGCAAAATGCTTGGAAACTGGTGAAACAGTTGCGATAAAAAAGGTTCTTCAGGACAAGAGGTATAAGAATAGGGAGTTGCAGACTATGCGTCTTCTAGACCACCCAAATGTCGTGTCCCTGAAGCATTGTTTCTTCTCAACGACAGAGAAGGATGAGCTTTATCTTAATCTTGTTCTTGAATATGTCCCTGAAACTGTTCATAGAGTGATCAAACATTACAACAAGATGAACCAAAGGATGCCACTGATTTACGTCAAACTCTATTTTTATCAGGTTTTTGCCGAACAGCCCTCTTCATTTTTGTGTATGGATAGTGAAGTTTTGTATTGAATCATATGCATATAAACTACTATATTTAAGATGAACGGGCCTTTCGAGAACATCTTTCTGTGCCTGGTTTTAATATTCTTTGTTTTCTTACTTTCGTTTCTACACAGATCTGCAGAGCTCTTTCATACATTCATAATAGTATTGGGGTATGCCACAGAGACATAAAGCCTCAAAAACTTACTGGTTAGTGTTCATTCTGTTTCTGGCGTCGATTAATATTTCGATAAGTAACTTGATTTTATATTTCAAATCCACAGATTTAATTGGTTTCCATTTCTGTCTGCTGATTGTTTTCACTTTCTCCTTTTGACAGGTTAATCCACACACCCACCAACTCAAGCTTTGTGACTTTGGCAGCGCAAAAGTCTTGGTTCGTTTTCTTTGAATTTTGGAAAATGTTCATTTTGTTTTGTGGTTCTACTTGTGACGTGGAGACTAATTTAGTTTACATATGGCAGGTTAGAGGCGAGCCAAATATAGCTTACATCTGTTCTAGATACTATAGAGCACCCGAACTCATATTTGGTGCGACCGAGTACACCACAGCCATCGACATCTGGTCAGCTGGTTGTGTTCTAGCTGAATTATTGCTTGGACAGGTTTGTAAAATTCCTCCCATGACCGCTGCATAACTATTATTACTATTAAGTAGAAACGATTCTAATAAGCGTTCTTGGACTCTTTCAGCCGCTATTTCCCGGTGAAAGTGGAGTTGATCAGCTTGTTGAGATAATCAAGGTATTAGGAGCTTTAATTTAATTTTTAAAGAGCTGTGAAGGTTTTCTTTGTTGGCAGTCTTTTCTATTCTTATTTTGATCCGACTTCAATGTTTAATTTGACAACTCTTGCAGGTTCTAGGAACCCCAACCAGGGAGGAAATTAAGTGCATGAACCCTAATTACACAGAATTCAAGTTTCCCCAAATTAAAGCTCATCCATGGCACAAGGTACTTAAAAAATCTTTGTACATATATGTTCCCTGTTCCTCCTTTTAAGTAATTTACCAATTTAACAGTGCATACATCCATGTTATCAGATATTCCATAAGAGAATGCCTCCAGAAGCTGTGGATCTCGTTTCAAGATTACTTCAGTACTCTCCAAACCTCCGAAGCACAGCGGTAAGTAGAAATATTTTAAAATTTCAGTCAACACTTGAATGCGGATACCCATTCGAGTCTCTGACTAATTTCTCTTCCACATTAATGCAGTTGGAGGCTTTGATTCATCCATTTTTTGATGAACTCCGTGACCCAAACACTCGTCTGCCAAACGGACGTTTCCTTCCTCCCTTATTCAACTTCAAGGCTCATGGTAAAAACCTCAAATTACTTGAGAACTACTGGTCCATCAGCTGTGATATATATGCCTAACTTTAGTTTATTTCAATTGTGTTGCCAACCAGAATTGAAGGGAGTACCCCCTGAGATGCTCGTGAGGCTTATTCCAGAACACGCAAGGAAACAATGCAATTTTCTTGGGTCGTAAAGCGGTCGGGTAGTAGTCTAAACGCTGCAGTGACACATAAGCCTGTAGAAAGTAGTAGCTAGCATAAAATTACAAATGGAAGCCCCTATTATTTGTTATTTTCTTCCCTGTTTAATTTGTTCCCATTCCCCTATTATTTTTTCATATCCATGGCCGCCGCCCCTTGTTTGTCCGTGTAGAAATCAAGTTGAGAGATGGTTCTGCTTGGTATGTTCTTAATACCCGCCCTTGATTTGTAGTTAACAGACAGATTGTAATTCCTTGTTGCAAATGAACATAACCTTGGAATATGCTCAGCAGCAATTTTGCCACTTCAATTACCATGTCATCAAGATTTGTGTACTGATGCCGCGAAATTCTTTATTGGTGGATGTTGAGCCAACTCCAATAACTTCAACTTTTCTGGTTTTCTAAGTTCTATTTTCCTCTTTTTGCGACTGAATTATTGTAGAACAAATCAAATCAGTACTTATTGCAGTACTAGAAATTTTTTCTAAATGAGGTATCATTTATTCAAGGAACCTTGATGGATGTTATTAGAGTAAGGAAATGTCAAAAGGTTGTCGGGGT

Coding sequence (CDS)

ATGACATCAGTAAGCGTAGCTCCAGCTTCTATGATAAGGGATCATGGTGGGAGCACAATCGGTGTTGACCGGCTGCCTGATGAGATGAATGATATGAAAATTAGGGATGATAAGGAAATTGAAGCCACCGTTGTTGATGGAAACGGTACTGAAACGGGCCATATAATTGTAACGACCATTGGTGGAAAGAATGGCCAACCAAAACAGACTATCAGTTACATGGCTGAGCGTGCTGTTGGACATGGTTCTTTTGGAGTTGTATTTCAGGCAAAATGCTTGGAAACTGGTGAAACAGTTGCGATAAAAAAGGTTCTTCAGGACAAGAGGTATAAGAATAGGGAGTTGCAGACTATGCGTCTTCTAGACCACCCAAATGTCGTGTCCCTGAAGCATTGTTTCTTCTCAACGACAGAGAAGGATGAGCTTTATCTTAATCTTGTTCTTGAATATGTCCCTGAAACTGTTCATAGAGTGATCAAACATTACAACAAGATGAACCAAAGGATGCCACTGATTTACGTCAAACTCTATTTTTATCAGATCTGCAGAGCTCTTTCATACATTCATAATAGTATTGGGGTATGCCACAGAGACATAAAGCCTCAAAAACTTACTGGTTAA

Protein sequence

MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTIGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQICRALSYIHNSIGVCHRDIKPQKLTG
Homology
[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match: P51139 (Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=2 SV=2)

HSP 1 Score: 364.4 bits (934), Expect = 8.6e-100
Identity = 181/205 (88.29%), Postives = 191/205 (93.17%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIE-ATVVDGNGTETGHIIVTT 60
           M S  VAPAS   D   S++GV++LP+EMNDMKIRDDKE+E AT+VDGNGTETGHIIVTT
Sbjct: 1   MASGGVAPASGFIDKNASSVGVEKLPEEMNDMKIRDDKEMEAATIVDGNGTETGHIIVTT 60

Query: 61  IGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
           IGGKNGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFY 180
           LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETV RVI+HYNKMNQRMP+IYVKLY Y
Sbjct: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVSRVIRHYNKMNQRMPMIYVKLYSY 180

Query: 181 QICRALSYIHNSIGVCHRDIKPQKL 205
           QICRAL+YIHNSIGVCHRDIKPQ L
Sbjct: 181 QICRALAYIHNSIGVCHRDIKPQNL 205

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match: P43288 (Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1 SV=3)

HSP 1 Score: 357.8 bits (917), Expect = 8.0e-98
Identity = 179/204 (87.75%), Postives = 187/204 (91.67%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV +AP    RD    + GVD+LP+EMNDMKIRDDKE+EATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVGIAPNPGARD----STGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPLIYVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 205
           I RALSYIH  IGVCHRDIKPQ L
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNL 200

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match: Q40518 (Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-97
Identity = 175/204 (85.78%), Postives = 189/204 (92.65%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSV +AP S +R+    ++GVDRLP+EMNDM+IRDDKEIEA +VDGNGTETGHIIVTTI
Sbjct: 1   MTSVGLAPVSGLRESSSHSVGVDRLPEEMNDMRIRDDKEIEAAIVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG++GQPKQTISYMAER VG GSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRHGQPKQTISYMAERIVGQGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVV LKHCFFSTTEKDE+YLNLVLEYVPETVHRVIKHYNK+NQRMPLI VKLY YQ
Sbjct: 121 LDHPNVVCLKHCFFSTTEKDEVYLNLVLEYVPETVHRVIKHYNKLNQRMPLILVKLYTYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 205
           I RALSYIH++IGVCHRDIKPQ L
Sbjct: 181 IFRALSYIHHTIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match: P51137 (Glycogen synthase kinase-3 homolog MsK-1 OS=Medicago sativa OX=3879 GN=MSK-1 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 4.4e-96
Identity = 179/206 (86.89%), Postives = 188/206 (91.26%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRD-HGGSTIGVDR-LPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVT 60
           M SV VAP S  R+  G   IGVD  LP+EM+DMKIRDD+E+EATVVDGNGTETGHIIVT
Sbjct: 1   MASVGVAPTSGFREVLGDGEIGVDDILPEEMSDMKIRDDREMEATVVDGNGTETGHIIVT 60

Query: 61  TIGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM 120
           TIGG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM
Sbjct: 61  TIGGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM 120

Query: 121 RLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYF 180
           RLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHY+K+NQRMP+IYVKLY 
Sbjct: 121 RLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYSKLNQRMPMIYVKLYT 180

Query: 181 YQICRALSYIHNSIGVCHRDIKPQKL 205
           YQI RALSYIH  IGVCHRDIKPQ L
Sbjct: 181 YQIFRALSYIHRCIGVCHRDIKPQNL 206

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match: P43289 (Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 7.5e-96
Identity = 170/204 (83.33%), Postives = 186/204 (91.18%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P++ +R+  G+    DRLP+EM DMKI+DDKE+EAT+V+GN TETGHIIVTTI
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 205
           I R+LSYIH  IGVCHRDIKPQ L
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match: XP_004148581.1 (glycogen synthase kinase-3 homolog MsK-3 [Cucumis sativus] >KAE8650916.1 hypothetical protein Csa_001894 [Cucumis sativus])

HSP 1 Score: 411 bits (1056), Expect = 1.38e-141
Identity = 203/204 (99.51%), Postives = 203/204 (99.51%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICRALSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match: KAA0038106.1 (glycogen synthase kinase-3-like protein MsK-3 [Cucumis melo var. makuwa] >TYK20502.1 glycogen synthase kinase-3-like protein MsK-3 [Cucumis melo var. makuwa])

HSP 1 Score: 409 bits (1052), Expect = 5.60e-141
Identity = 202/204 (99.02%), Postives = 203/204 (99.51%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVAPAS+IRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVAPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICRALSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match: XP_038905663.1 (glycogen synthase kinase-3 homolog MsK-3 isoform X2 [Benincasa hispida])

HSP 1 Score: 408 bits (1048), Expect = 2.27e-140
Identity = 201/204 (98.53%), Postives = 202/204 (99.02%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV PAS+IRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICRALSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match: XP_038905662.1 (glycogen synthase kinase-3 homolog MsK-3 isoform X1 [Benincasa hispida])

HSP 1 Score: 408 bits (1048), Expect = 6.20e-140
Identity = 201/204 (98.53%), Postives = 202/204 (99.02%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV PAS+IRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICRALSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match: XP_022983441.1 (glycogen synthase kinase-3 homolog MsK-3-like [Cucurbita maxima])

HSP 1 Score: 402 bits (1033), Expect = 4.32e-138
Identity = 197/204 (96.57%), Postives = 201/204 (98.53%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV P S+IRDHGGSTIGVDRLPDEMNDM+IRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICR+L+YIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match: A0A5D3DAA6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00760 PE=3 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 2.71e-141
Identity = 202/204 (99.02%), Postives = 203/204 (99.51%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVAPAS+IRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVAPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICRALSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match: A0A6J1J277 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111482043 PE=3 SV=1)

HSP 1 Score: 402 bits (1033), Expect = 2.09e-138
Identity = 197/204 (96.57%), Postives = 201/204 (98.53%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV P S+IRDHGGSTIGVDRLPDEMNDM+IRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICR+L+YIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match: A0A6J1FAG2 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111442293 PE=3 SV=1)

HSP 1 Score: 402 bits (1033), Expect = 2.09e-138
Identity = 197/204 (96.57%), Postives = 201/204 (98.53%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV P S+IRDHGGSTIGVDRLPDEMNDM+IRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICR+L+YIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match: A0A6J1DLW7 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111022381 PE=3 SV=1)

HSP 1 Score: 400 bits (1027), Expect = 1.70e-137
Identity = 196/204 (96.08%), Postives = 200/204 (98.04%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV PAS I DHGGSTIGV+RLPDEMNDMK+RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICR+L+YIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNL 204

[Close]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match: A0A6J1GBR5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452723 PE=3 SV=1)

HSP 1 Score: 399 bits (1025), Expect = 3.43e-137
Identity = 196/204 (96.08%), Postives = 199/204 (97.55%), Query Frame = 0 

Query: 1   MTSVSVAPASMIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SVSV PA +IRD GGSTIGVDRLPDEMNDMK+RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVSVVPAPVIRDRGGSTIGVDRLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRALSYIHNSIGVCHRDIKPQKL 204
           ICR+LSYIHNSIGVCHRDIKPQ L
Sbjct: 181 ICRSLSYIHNSIGVCHRDIKPQNL 204

The following BLAST results are available for this feature:
BLAST of Cucsat.G12337.T5 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P511398.6e-10088.29Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=... [more]
P432888.0e-9887.75Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1... [more]
Q405181.0e-9785.78Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 S... [more]
P511374.4e-9686.89Glycogen synthase kinase-3 homolog MsK-1 OS=Medicago sativa OX=3879 GN=MSK-1 PE=... [more]
P432897.5e-9683.33Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1... [more]
BLAST of Cucsat.G12337.T5 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004148581.11.38e-14199.51glycogen synthase kinase-3 homolog MsK-3 [Cucumis sativus] >KAE8650916.1 hypothe... [more]
KAA0038106.15.60e-14199.02glycogen synthase kinase-3-like protein MsK-3 [Cucumis melo var. makuwa] >TYK205... [more]
XP_038905663.12.27e-14098.53glycogen synthase kinase-3 homolog MsK-3 isoform X2 [Benincasa hispida][more]
XP_038905662.16.20e-14098.53glycogen synthase kinase-3 homolog MsK-3 isoform X1 [Benincasa hispida][more]
XP_022983441.14.32e-13896.57glycogen synthase kinase-3 homolog MsK-3-like [Cucurbita maxima][more]
BLAST of Cucsat.G12337.T5 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DAA62.71e-14199.02Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1J2772.09e-13896.57Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FAG22.09e-13896.57Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DLW71.70e-13796.08Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1GBR53.43e-13796.08Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 73..205
e-value: 2.1E-7
score: -29.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 73..202
e-value: 1.5E-25
score: 90.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 73..206
score: 22.859638
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 57..152
e-value: 2.9E-36
score: 125.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 153..205
e-value: 6.6E-11
score: 44.0
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 106..203
e-value: 5.4E-6
score: 21.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR24057:SF65SHAGGY-RELATED PROTEIN KINASE ALPHAcoord: 1..204
NoneNo IPR availablePANTHERPTHR24057GLYCOGEN SYNTHASE KINASE-3 ALPHAcoord: 1..204
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 79..103
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 72..202

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G12337Cucsat.G12337gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12337.T5.E1Cucsat.G12337.T5.E1exon
Cucsat.G12337.T5.E2Cucsat.G12337.T5.E2exon
Cucsat.G12337.T5.E3Cucsat.G12337.T5.E3exon
Cucsat.G12337.T5.E4Cucsat.G12337.T5.E4exon
Cucsat.G12337.T5.E5Cucsat.G12337.T5.E5exon
Cucsat.G12337.T5.E6Cucsat.G12337.T5.E6exon
Cucsat.G12337.T5.E7Cucsat.G12337.T5.E7exon
Cucsat.G12337.T5.E8Cucsat.G12337.T5.E8exon
Cucsat.G12337.T5.E9Cucsat.G12337.T5.E9exon
Cucsat.G12337.T5.E10Cucsat.G12337.T5.E10exon
Cucsat.G12337.T5.E11Cucsat.G12337.T5.E11exon
Cucsat.G12337.T5.E12Cucsat.G12337.T5.E12exon
Cucsat.G12337.T5.E13Cucsat.G12337.T5.E13exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12337.T5.C1Cucsat.G12337.T5.C1CDS
Cucsat.G12337.T5.C2Cucsat.G12337.T5.C2CDS
Cucsat.G12337.T5.C3Cucsat.G12337.T5.C3CDS
Cucsat.G12337.T5.C4Cucsat.G12337.T5.C4CDS
Cucsat.G12337.T5.C5Cucsat.G12337.T5.C5CDS
Cucsat.G12337.T5.C6Cucsat.G12337.T5.C6CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G12337.T5Cucsat.G12337.T5-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity