Cucsat.G12209.T7 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12209.T7
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING-type E3 ubiquitin transferase
Location	ctg1837: 2226979 .. 2229925 (-)
RNA-Seq Expression	Cucsat.G12209.T7
Synteny	Cucsat.G12209.T7

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTGACGACTGTACGGAGGAAGCGAATCACATAGTTTTCCTCTTTGTAGGTTAGCTTCTTTCTGAATGTAATGTTGAATCTGGAAGATTTCCAATGTATGAGTGCCACAATTAATTAGGGTTTTATGGCAAAACTTTACTTTCGTTTCAATCTCAGAATTCTTCTCCTCAAAGTGGGATGTTTCTGCATTTCGTTTTCAATTTTCCGACTGCTTTGTTTATCAGCCTTCTCTGTTTTATTCATATTTTACATATTTTTTTTGCGCTATTGACAAATTCTCATCACACTTTGTTTCTTAAGATTGGGTTTCTGAAGAGGGAGGCAACTAGGTTGATTCTTGTTTAGGGAAGGAATTCGAGGGGGAATTTTCAAAGGGGATAAATATGCTTCAGATCCGGCTTAGCAAAGTTCCACTGGCTGAAAGTGGTGGTGGGGCAGTAAAGCCCTTGCCGGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTCGTTCTTGCTGATCTTCCTGTAGCAAAGGGCATTGGTGCGGCTACCGCTGCTTCCATTGTTAAGTCTGTGGGTCGTAGGTCTCGCCGGCAGCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCTATTGCAATTTATGGTCGCCTGGTATTTTTCTCCCCAAACTTCTTGTTATTTTTGCTAGTTGCTTTGTTTACTTTTGGTCCATGGAATCATTCATATATAAAACACTGTTTTTAATTTGGTTTTTATTTTTCCAAATTGTTTTGCATCACTTTTATCAAATATATGGGTACTGGCTGCTTGTCAATTTATAGAAATATAATGGAGGAGGCTAAGAGCTATATCAATTCGATGTGGTCATTTTATTCATATCTTGCAATGTCCTTGCCTCTCTTGATTGGAGGCCTTTGACATGCTTCTCAAGAGCTAAATTGTAGTTTGCCTTTCTTGCAGAGTCCTTGTGAGCATGCTTTCTGTCTTGATTGTGCAAGGAGCGATTCTTTATGCTACCTGTAAGTAAAAAGGCTTTCAAGGTCATAACGAGACCATCAAAAGATTCTCTGGTGTCCAGAATGTCGAAAGTGATTGCTTAAATTTAGTGCATTACTCTTATACAAAATAAAAATTTAGTACATTGCATTTTTTCCTCGGCTATTGCAAAAACTTTCGCTCTTTAAATATCAGTTGTTTTCTTTGCTGATTAGTTACTATACTACACAGTTTTCTAGTAAAAGCAGGATTCAGCTTTTCCTTTTGTCTTTACTAAGATAATTTATGGAGCATGAATTTAAAGCACCAAATTGAAGATCAGTCAATGATATTTGTAGTGGACATACTCATGTCTGTTTGAATTGAAATTTGATCAACAAAGAATCATATATATATAGTATACTCAAAGCCATCCTTGTGCCATTTCAATGTTAAAAACAAAGCTGCTGTGACTGACAGATCAGCAATAGTTACACTGTGAACCATCAAGTCTCGTTTTATTGTAATTTTTACAGATATTGATACTTGTGTACTTCATTGTAATGTTCTGAAGATGACATCAGAATTGATTTTTGTGACACAGTTGTGATGATCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACACTGTCTCAAGTCTTTTCTTAAGAGGTCTGAATTTGAATCTCATATACACGAGAACCATGCTGACCTTCTTAAGCCAAATGCAGACAAGGAAGATGGAAATGAAATTGAGGCTAATAGTGCGAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCACTGAGACCTCTCATATCTCCGGGTTCCAATTCCCAGCCTCAAGAACGTGATGAAAAGTTTCATCGTCAACAATCTAGAGACCAACCCAGATCTGGCATGCAGCAAAAGCAAACGCCAAGTTTTGGTCAGAACCAAAACAATACATCTGAGTCTCAACAAGACAGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCTCAAAACTTCGATGCACAGGGTGCTCCACATCAGGACTCCAGTCAGTTTCCAGAGAAGCAGCAAGGAATTCTATCCGATACTCCATATTCACAGTATCCTCCTTTACAACCAATTCCACCACCAAATTATGTGGTTCCGGCCAACTCCAATCCAATGTTGACTCCCCCTCTACCATTTGGTTATCCCCCTTTTCCAATTGAGGGAGCACAACAATATTACAATACACCCTATGAAGTATCAAGGCAAGACACTGCAGCAGAGACCGGATCCGAGCAAGGGTCACTCCTAGGGTTTCCACCAGGTGCGGCAGGAGGAATGAATTTCTCAGCAACGTATCCACAGTCCTGGAATACTGCACAAGCTGGCATTCCTTATGAGCATGCTGGTGGAGGTCAAGGAGATTATAGACGCAGCCCTGGACGTATGCCTGTGAATTCTTCTGCAGGTAATGCAATGGACATACGGGATGGTAAAGGTATATTGGCACCACAGCCTCTAATACAATTGCCTCCACCTCCACCGCCCCCACCATACATGTCACATAACAAACGTGGTAAGTTTTATTCAGGCGATATGGATCATGATGGGCAAAGCCTTGGATGGCAGAATGACAGCCATAGTCGAGATAGCTTTGGAAGTGGCCAAGATTGAATATTTTTTTTCCCCATGGCATTCTTTATGTCCTTTGCCATATAGTTCCAATCATTGTTTTGATAAAAATTTGCATCAGCTTGATAATGTCTGTATTTGAACCTTGATGCTCGATGGTAAAAGTTCTGCCCTTGGAGGCCTTTTTGAGTACAAACATTGTCATTTGAGAGGTTCGAGATCAATATTATATGCAATAGTGTACAGTAGATAAGGATTAAGAGTATTATCATCATTGAAGGAGTTCTGGAAAGTTTTTGGTAAAAGTATTATAACGTTTAT

Coding sequence (CDS)

ATGGAGGGGATCTTCATTTGTGCAGCCCCACACTGTCTCAAGTCTTTTCTTAAGAGGTCTGAATTTGAATCTCATATACACGAGAACCATGCTGACCTTCTTAAGCCAAATGCAGACAAGGAAGATGGAAATGAAATTGAGGCTAATAGTGCGAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCACTGAGACCTCTCATATCTCCGGGTTCCAATTCCCAGCCTCAAGAACGTGATGAAAAGTTTCATCGTCAACAATCTAGAGACCAACCCAGATCTGGCATGCAGCAAAAGCAAACGCCAAGTTTTGGTCAGAACCAAAACAATACATCTGAGTCTCAACAAGACAGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCTCAAAACTTCGATGCACAGGGTGCTCCACATCAGGACTCCAGTCAGTTTCCAGAGAAGCAGCAAGGAATTCTATCCGATACTCCATATTCACAGTATCCTCCTTTACAACCAATTCCACCACCAAATTATGTGGTTCCGGCCAACTCCAATCCAATGTTGACTCCCCCTCTACCATTTGGTTATCCCCCTTTTCCAATTGAGGGAGCACAACAATATTACAATACACCCTATGAAGTATCAAGGCAAGACACTGCAGCAGAGACCGGATCCGAGCAAGGGTCACTCCTAGGGTTTCCACCAGGTGCGGCAGGAGGAATGAATTTCTCAGCAACGTATCCACAGTCCTGGAATACTGCACAAGCTGGCATTCCTTATGAGCATGCTGGTGGAGGTCAAGGAGATTATAGACGCAGCCCTGGACGTATGCCTGTGAATTCTTCTGCAGGTAATGCAATGGACATACGGGATGGTAAAGGTATATTGGCACCACAGCCTCTAATACAATTGCCTCCACCTCCACCGCCCCCACCATACATGTCACATAACAAACGTGGTAAGTTTTATTCAGGCGATATGGATCATGATGGGCAAAGCCTTGGATGGCAGAATGACAGCCATAGTCGAGATAGCTTTGGAAGTGGCCAAGATTGA

Protein sequence

MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTVRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD

Homology

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BLAST of Cucsat.G12209.T7 vs. ExPASy Swiss-Prot
Match: Q9LFC0 (E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAKAI PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.3e-27
Identity = 99/264 (37.50%), Postives = 136/264 (51.52%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSA-KQSTASES 60
           MEGIFICAAPHCL+SFLK+ +FE+H+H+ H  LL+ +A+KEDGN+ +  S  +QS+ASES
Sbjct: 119 MEGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASES 178

Query: 61  TVRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDS 120
           T+R PLR  +                 QQSR+  RS    K    F Q QN   +S  D+
Sbjct: 179 TLRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQVQNYPPDS--DN 238

Query: 121 GHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNY 180
               GF+   P                +   +FP+  Q            P+  + PP+ 
Sbjct: 239 SRPPGFETASP----------------KPGIRFPDYPQ------------PMNLMQPPSL 298

Query: 181 VVPANSNPMLTPPLPFGYPPFPIE---GAQQYYN-TPYEVSRQDTAAETGSEQGSLLGF- 240
            VP N NP L  P  FG+P +P      +QQ++N   YE++R ++    GSEQ SLLG+ 
Sbjct: 299 PVPMNQNPGL--PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYP 330

Query: 241 PPGAAGGMNFSATY-PQSWNTAQA 258
           PP     +NF  +Y P SWN   A
Sbjct: 359 PPSPMTNLNFQGSYPPPSWNPGMA 330

[Close]

BLAST of Cucsat.G12209.T7 vs. NCBI nr
Match: XP_004137915.1 (E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >XP_031738067.1 E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >KGN58802.1 hypothetical protein Csa_000851 [Cucumis sativus])

HSP 1 Score: 703 bits (1815), Expect = 1.47e-253
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG
Sbjct: 300 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 300
           GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ
Sbjct: 360 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 419

Query: 301 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 352
           PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 420 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209.T7 vs. NCBI nr
Match: XP_008442473.1 (PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >XP_008442475.1 PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >KAA0044143.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa] >TYK24994.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa])

HSP 1 Score: 690 bits (1780), Expect = 3.14e-248
Identity = 346/352 (98.30%), Postives = 348/352 (98.86%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA G
Sbjct: 300 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 300
           GMNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ
Sbjct: 360 GMNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 419

Query: 301 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 352
           PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 420 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209.T7 vs. NCBI nr
Match: XP_038905987.1 (E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida] >XP_038905988.1 E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida])

HSP 1 Score: 663 bits (1710), Expect = 2.96e-237
Identity = 336/372 (90.32%), Postives = 344/372 (92.47%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGN+IEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNDIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPL+SPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQTP+FG NQNNTSESQQDSG
Sbjct: 180 VRGPLRPLVSPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQTPNFGPNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQF EKQQGIL+DTPYSQYPPLQ IPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFSEKQQGILADTPYSQYPPLQSIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNP+LTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG
Sbjct: 300 VPANSNPILTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 300
           GMNFSATYPQ WNT Q GIPYEHAGGGQG                    DYRRSPGRMPV
Sbjct: 360 GMNFSATYPQPWNTGQGGIPYEHAGGGQGMPDAFANTPDSQGKHGFYQGDYRRSPGRMPV 419

Query: 301 NSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQND 352
           NSS GNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKR KFYSGDMDHDGQSLGWQND
Sbjct: 420 NSSVGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRVKFYSGDMDHDGQSLGWQND 479

[Close]

BLAST of Cucsat.G12209.T7 vs. NCBI nr
Match: XP_023527487.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616 bits (1588), Expect = 1.86e-218
Identity = 316/387 (81.65%), Postives = 336/387 (86.82%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++
Sbjct: 120 MEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETS 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQ+S QF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQESGQFSEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAA 240
           VP NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAA
Sbjct: 300 VPPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAA 359

Query: 241 GGMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMP 300
           GGMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MP
Sbjct: 360 GGMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMP 419

Query: 301 VNSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYS 352
           VNS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY 
Sbjct: 420 VNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYP 479

[Close]

BLAST of Cucsat.G12209.T7 vs. NCBI nr
Match: KAG6580495.1 (E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017247.1 E3 ubiquitin-protein ligase Hakai [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 610 bits (1573), Expect = 3.56e-216
Identity = 314/387 (81.14%), Postives = 334/387 (86.30%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++
Sbjct: 120 MEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETS 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNY 
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYA 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAA 240
           VP NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAA
Sbjct: 300 VPPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAA 359

Query: 241 GGMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMP 300
           GGMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MP
Sbjct: 360 GGMNFSATYPHPWNAGQAGIPYDHATGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMP 419

Query: 301 VNSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYS 352
           VNS+              +G+ MD+RDGKGILAPQPLIQLPPPP PP ++SHNKRGKFY 
Sbjct: 420 VNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPAFLSHNKRGKFYP 479

[Close]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL
Match: A0A0A0LDI2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G732590 PE=4 SV=1)

HSP 1 Score: 703 bits (1815), Expect = 7.10e-254
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG
Sbjct: 300 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 300
           GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ
Sbjct: 360 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 419

Query: 301 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 352
           PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 420 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL
Match: A0A1S3B5R9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486330 PE=4 SV=1)

HSP 1 Score: 690 bits (1780), Expect = 1.52e-248
Identity = 346/352 (98.30%), Postives = 348/352 (98.86%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA G
Sbjct: 300 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 300
           GMNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ
Sbjct: 360 GMNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 419

Query: 301 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 352
           PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 420 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL
Match: A0A5A7TKW6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001120 PE=4 SV=1)

HSP 1 Score: 690 bits (1780), Expect = 1.52e-248
Identity = 346/352 (98.30%), Postives = 348/352 (98.86%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASEST
Sbjct: 120 MEGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASEST 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAG 240
           VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA G
Sbjct: 300 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPG 359

Query: 241 GMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 300
           GMNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ
Sbjct: 360 GMNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQ 419

Query: 301 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 352
           PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 420 PLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL
Match: A0A6J1J5I8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 PE=4 SV=1)

HSP 1 Score: 608 bits (1568), Expect = 9.92e-216
Identity = 314/387 (81.14%), Postives = 334/387 (86.30%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++
Sbjct: 120 MEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETS 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VR PLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQ NTSESQQDSG
Sbjct: 180 VRCPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQINTSESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAA 240
           VP NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAA
Sbjct: 300 VPPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAA 359

Query: 241 GGMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMP 300
           GGMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MP
Sbjct: 360 GGMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMP 419

Query: 301 VNSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYS 352
           VNS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY 
Sbjct: 420 VNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYP 479

[Close]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL
Match: A0A6J1F6T3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395 PE=4 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 1.41e-215
Identity = 312/387 (80.62%), Postives = 334/387 (86.30%), Query Frame = 0


Query: 1   MEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASEST 60
           MEGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++
Sbjct: 120 MEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETS 179

Query: 61  VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 120
           VRGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSG
Sbjct: 180 VRGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSG 239

Query: 121 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 180
           HSQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYV
Sbjct: 240 HSQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYV 299

Query: 181 VPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAA 240
           VP NSNPMLTPPLPFGYPPFP+EGAQ+YY++PYEV RQDTAA ETGSEQGSLLGFPPGAA
Sbjct: 300 VPPNSNPMLTPPLPFGYPPFPVEGAQKYYSSPYEVPRQDTAAAETGSEQGSLLGFPPGAA 359

Query: 241 GGMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMP 300
           GGMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MP
Sbjct: 360 GGMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMP 419

Query: 301 VNSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYS 352
           VNS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY 
Sbjct: 420 VNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYP 479

The following BLAST results are available for this feature:

BLAST of Cucsat.G12209.T7 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFC0	3.3e-27	37.50	E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAK...	[more]

BLAST of Cucsat.G12209.T7 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137915.1	1.47e-253	100.00	E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >XP_031738067.1 E3 u...	[more]
XP_008442473.1	3.14e-248	98.30	PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >XP_008442475.1 PRED...	[more]
XP_038905987.1	2.96e-237	90.32	E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida] >XP_038905988.1 E3...	[more]
XP_023527487.1	1.86e-218	81.65	E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo]	[more]
KAG6580495.1	3.56e-216	81.14	E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma ...	[more]

BLAST of Cucsat.G12209.T7 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDI2	7.10e-254	100.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G732590 PE...	[more]
A0A1S3B5R9	1.52e-248	98.30	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486330 PE=4 ...	[more]
A0A5A7TKW6	1.52e-248	98.30	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1J5I8	9.92e-216	81.14	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 P...	[more]
A0A6J1F6T3	1.41e-215	80.62	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..181
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 332..352
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 298..352
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..167
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..123
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..316
None	No IPR available	PANTHER	PTHR13480:SF0	E3 UBIQUITIN-PROTEIN LIGASE HAKAI	coord: 1..320
IPR040383	E3 ubiquitin-protein ligase HAKAI/CBLL2	PANTHER	PTHR13480	E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED	coord: 1..320
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 7..30
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 5..31 score: 8.600363

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12209	Cucsat.G12209	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12209.T7.E1	Cucsat.G12209.T7.E1	exon
Cucsat.G12209.T7.E2	Cucsat.G12209.T7.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12209.T7.C1	Cucsat.G12209.T7.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12209.T7	Cucsat.G12209.T7-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0030155	regulation of cell adhesion
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0046872	metal ion binding