Cucsat.G12209 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G12209
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING-type E3 ubiquitin transferase
Location	ctg1837: 2227004 .. 2230006 (-)
RNA-Seq Expression	Cucsat.G12209
Synteny	Cucsat.G12209

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCAACGGGGTTCTTAAGACGACGCGTCGTATGAAAGTTTAGTGGCGCCAGAAAGTTGACGACGTGTCGTTCTATTCACTGATATATCGGAATCGGACAACCCTTCTTCTTCCATTTGGGAGCGAAGAAGCGAAAGGACGAAGGACGGACTGACGACTGTACGGAGGAAGCGAATCACATAGTTTTCCTCTTTGTAGGTTAGCTTCTTTCTGAATGTAATGTTGAATCTGGAAGATTTCCAATGTATGAGTGCCACAATTAATTAGGGTTTTATGGCAAAACTTTACTTTCGTTTCAATCTCAGAATTCTTCTCCTCAAAGTGGGATGTTTCTGCATTTCGTTTTCAATTTTCCGACTGCTTTGTTTATCAGCCTTCTCTGTTTTATTCATATTTTACATATTTTTTTTGCGCTATTGACAAATTCTCATCACACTTTGTTTCTTAAGATTGGGTTTCTGAAGAGGGAGGCAACTAGGTTGATTCTTGTTTAGGGAAGGAATTCGAGGGGGAATTTTCAAAGGGGATAAATATGCTTCAGATCCGGCTTAGCAAAGTTCCACTGGCTGAAAGTGGTGGTGGGGCAGTAAAGCCCTTGCCGGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTCGTTCTTGCTGATCTTCCTGTAGCAAAGGGCATTGGTGCGGCTACCGCTGCTTCCATTGTTAAGTCTGTGGGTCGTAGGTCTCGCCGGCAGCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCTATTGCAATTTATGGTCGCCTGGTATTTTTCTCCCCAAACTTCTTGTTATTTTTGCTAGTTGCTTTGTTTACTTTTGGTCCATGGAATCATTCATATATAAAACACTGTTTTTAATTTGGTTTTTATTTTTCCAAATTGTTTTGCATCACTTTTATCAAATATATGGGTACTGGCTGCTTGTCAATTTATAGAAATATAATGGAGGAGGCTAAGAGCTATATCAATTCGATGTGGTCATTTTATTCATATCTTGCAATGTCCTTGCCTCTCTTGATTGGAGGCCTTTGACATGCTTCTCAAGAGCTAAATTGTAGTTTGCCTTTCTTGCAGAGTCCTTGTGAGCATGCTTTCTGTCTTGATTGTGCAAGGAGCGATTCTTTATGCTACCTGTAAGTAAAAAGGCTTTCAAGGTCATAACGAGACCATCAAAAGATTCTCTGGTGTCCAGAATGTCGAAAGTGATTGCTTAAATTTAGTGCATTACTCTTATACAAAATAAAAATTTAGTACATTGCATTTTTTCCTCGGCTATTGCAAAAACTTTCGCTCTTTAAATATCAGTTGTTTTCTTTGCTGATTAGTTACTATACTACACAGTTTTCTAGTAAAAGCAGGATTCAGCTTTTCCTTTTGTCTTTACTAAGATAATTTATGGAGCATGAATTTAAAGCACCAAATTGAAGATCAGTCAATGATATTTGTAGTGGACATACTCATGTCTGTTTGAATTGAAATTTGATCAACAAAGAATCATATATATATAGTATACTCAAAGCCATCCTTGTGCCATTTCAATGTTAAAAACAAAGCTGCTGTGACTGACAGATCAGCAATAGTTACACTGTGAACCATCAAGTCTCGTTTTATTGTAATTTTTACAGATATTGATACTTGTGTACTTCATTGTAATGTTCTGAAGATGACATCAGAATTGATTTTTGTGACACAGTTGTGATGATCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACACTGTCTCAAGTCTTTTCTTAAGAGGTCTGAATTTGAATCTCATATACACGAGAACCATGCTGACCTTCTTAAGCCAAATGCAGACAAGGAAGATGGAAATGAAATTGAGGCTAATAGTGCGAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCACTGAGACCTCTCATATCTCCGGGTTCCAATTCCCAGCCTCAAGAACGTGATGAAAAGTTTCATCGTCAACAATCTAGAGACCAACCCAGATCTGGCATGCAGCAAAAGCAAACGCCAAGTTTTGGTCAGAACCAAAACAATACATCTGAGTCTCAACAAGACAGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCTCAAAACTTCGATGCACAGGGTGCTCCACATCAGGACTCCAGTCAGTTTCCAGAGAAGCAGCAAGGAATTCTATCCGATACTCCATATTCACAGTATCCTCCTTTACAACCAATTCCACCACCAAATTATGTGGTTCCGGCCAACTCCAATCCAATGTTGACTCCCCCTCTACCATTTGGTTATCCCCCTTTTCCAATTGAGGGAGCACAACAATATTACAATACACCCTATGAAGTATCAAGGCAAGACACTGCAGCAGAGACCGGATCCGAGCAAGGGTCACTCCTAGGGTTTCCACCAGGTGCGGCAGGAGGAATGAATTTCTCAGCAACGTATCCACAGTCCTGGAATACTGCACAAGCTGGCATTCCTTATGAGCATGCTGGTGGAGGTCAAGGAGATTATAGACGCAGCCCTGGACGTATGCCTGTGAATTCTTCTGCAGGTAATGCAATGGACATACGGGATGGTAAAGGTATATTGGCACCACAGCCTCTAATACAATTGCCTCCACCTCCACCGCCCCCACCATACATGTCACATAACAAACGTGGTAAGTTTTATTCAGGCGATATGGATCATGATGGGCAAAGCCTTGGATGGCAGAATGACAGCCATAGTCGAGATAGCTTTGGAAGTGGCCAAGATTGAATATTTTTTTTCCCCATGGCATTCTTTATGTCCTTTGCCATATAGTTCCAATCATTGTTTTGATAAAAATTTGCATCAGCTTGATAATGTCTGTATTTGAACCTTGATGCTCGATGGTAAAAGTTCTGCCCTTGGAGGCCTTTTTGAGTACAAACATTGTCATTTGAGAGGTTCGAGATCAATATTATATGCAATAGTGTACAGTAGATAAGGATTAAGAGTATTATCATCATTGAAGGAGTTCTGGAAAGTTTTTGGTAAAAGTATTATAACGTTTATCAACTCTGACATTGCTTATTGAAT

Coding sequence (CDS)

ATGCTTCAGATCCGGCTTAGCAAAGTTCCACTGGCTGAAAGTGGTGGTGGGGCAGTAAAGCCCTTGCCGGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTCGTTCTTGCTGATCTTCCTGTAGCAAAGGGCATTGGTGCGGCTACCGCTGCTTCCATTGTTAAGTCTGTGGGTCGTAGGTCTCGCCGGCAGCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCTATTGCAATTTATGGTCGCCTGAGTCCTTGTGAGCATGCTTTCTGTCTTGATTGTGCAAGGAGCGATTCTTTATGCTACCTTTGTGATGATCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACACTGTCTCAAGTCTTTTCTTAAGAGGTCTGAATTTGAATCTCATATACACGAGAACCATGCTGACCTTCTTAAGCCAAATGCAGACAAGGAAGATGGAAATGAAATTGAGGCTAATAGTGCGAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCACTGAGACCTCTCATATCTCCGGGTTCCAATTCCCAGCCTCAAGAACGTGATGAAAAGTTTCATCGTCAACAATCTAGAGACCAACCCAGATCTGGCATGCAGCAAAAGCAAACGCCAAGTTTTGGTCAGAACCAAAACAATACATCTGAGTCTCAACAAGACAGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCTCAAAACTTCGATGCACAGGGTGCTCCACATCAGGACTCCAGTCAGTTTCCAGAGAAGCAGCAAGGAATTCTATCCGATACTCCATATTCACAGTATCCTCCTTTACAACCAATTCCACCACCAAATTATGTGGTTCCGGCCAACTCCAATCCAATGTTGACTCCCCCTCTACCATTTGGTTATCCCCCTTTTCCAATTGAGGGAGCACAACAATATTACAATACACCCTATGAAGTATCAAGGCAAGACACTGCAGCAGAGACCGGATCCGAGCAAGGGTCACTCCTAGGGTTTCCACCAGGTGCGGCAGGAGGAATGAATTTCTCAGCAACGTATCCACAGTCCTGGAATACTGCACAAGCTGGCATTCCTTATGAGCATGCTGGTGGAGGTCAAGGAGATTATAGACGCAGCCCTGGACGTATGCCTGTGAATTCTTCTGCAGGTAATGCAATGGACATACGGGATGGTAAAGGTATATTGGCACCACAGCCTCTAATACAATTGCCTCCACCTCCACCGCCCCCACCATACATGTCACATAACAAACGTGGTAAGTTTTATTCAGGCGATATGGATCATGATGGGCAAAGCCTTGGATGGCAGAATGACAGCCATAGTCGAGATAGCTTTGGAAGTGGCCAAGATTGA

Protein sequence

MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGRRSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLMEGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTVRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD

Homology

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BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot
Match: Q9LFC0 (E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAKAI PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.4e-84
Identity = 192/383 (50.13%), Postives = 241/383 (62.92%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRL +    E+G GA +P P ETVTVACPDHLVLADLPVAKGIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNGA-RPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRL PC+HAFCL+CARSDS+CYLCD+RIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSA-KQSTASEST 180
           EGIFICAAPHCL+SFLK+ +FE+H+H+ H  LL+ +A+KEDGN+ +  S  +QS+ASEST
Sbjct: 121 EGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASEST 180

Query: 181 VRGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSG 240
           +R PLR  +                 QQSR+  RS    K    F Q QN   +S  D+ 
Sbjct: 181 LRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQVQNYPPDS--DNS 240

Query: 241 HSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYV 300
              GF+   P                +   +FP+  Q            P+  + PP+  
Sbjct: 241 RPPGFETASP----------------KPGIRFPDYPQ------------PMNLMQPPSLP 300

Query: 301 VPANSNPMLTPPLPFGYPPFPIE---GAQQYYN-TPYEVSRQDTAAETGSEQGSLLGF-P 360
           VP N NP L  P  FG+P +P      +QQ++N   YE++R ++    GSEQ SLLG+ P
Sbjct: 301 VPMNQNPGL--PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYPP 330

Query: 361 PGAAGGMNFSATY-PQSWNTAQA 377
           P     +NF  +Y P SWN   A
Sbjct: 361 PSPMTNLNFQGSYPPPSWNPGMA 330

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BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot
Match: Q75N03 (E3 ubiquitin-protein ligase Hakai OS=Homo sapiens OX=9606 GN=CBLL1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.8e-07
Identity = 76/283 (26.86%), Postives = 111/283 (39.22%), Query Frame = 0


Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCEHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI IYGR+ PC+H FC DCA     + D +C  C D +Q+I+ 
Sbjct: 98  LGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQ 157

Query: 126 IKLMEGIFICA-APHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTA 185
                 +F+C+    C +++L + + ++HI+  H    KP       N     +   +  
Sbjct: 158 C-TRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEI 217

Query: 186 SESTVRGPLRPLIS------------PGSNSQPQERDEKFHR---------QQSRDQPRS 245
            E  +  P +  +S            P     P E   + H            +   PRS
Sbjct: 218 PERFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRS 277

Query: 246 GMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEK 305
             Q+    S  ++ N  +   QD  +S          R PP    A    H      PE 
Sbjct: 278 VSQETFRISTRKHSNLITVPIQDDSNS--------GAREPPPPAPAPAHHH------PEY 337

Query: 306 QQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYP 318
           Q   +   P+   PP Q   PP    P  S+PM  PP   G P
Sbjct: 338 QGQPVVSHPHHIMPPQQHYAPPPPPPPPISHPMPHPPQAAGTP 365

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BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot
Match: Q4R7I8 (E3 ubiquitin-protein ligase Hakai OS=Macaca fascicularis OX=9541 GN=CBLL1 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.8e-07
Identity = 76/283 (26.86%), Postives = 111/283 (39.22%), Query Frame = 0


Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCEHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI IYGR+ PC+H FC DCA     + D +C  C D +Q+I+ 
Sbjct: 97  LGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQ 156

Query: 126 IKLMEGIFICA-APHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTA 185
                 +F+C+    C +++L + + ++HI+  H    KP       N     +   +  
Sbjct: 157 C-TRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEI 216

Query: 186 SESTVRGPLRPLIS------------PGSNSQPQERDEKFHR---------QQSRDQPRS 245
            E  +  P +  +S            P     P E   + H            +   PRS
Sbjct: 217 PERFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRS 276

Query: 246 GMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEK 305
             Q+    S  ++ N  +   QD  +S          R PP    A    H      PE 
Sbjct: 277 VSQETFRISTRKHSNLITVPIQDDSNS--------GAREPPPPAPAPAHHH------PEY 336

Query: 306 QQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYP 318
           Q   +   P+   PP Q   PP    P  S+PM  PP   G P
Sbjct: 337 QGQPVVSHPHHIMPPQQHYAPPPPPPPPISHPMPHPPQAAGTP 364

[Close]

BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot
Match: Q5ZHZ4 (E3 ubiquitin-protein ligase Hakai OS=Gallus gallus OX=9031 GN=CBLL1 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 4.3e-06
Identity = 98/412 (23.79%), Postives = 153/412 (37.14%), Query Frame = 0


Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCEHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI +YGR+ PC+H FC DCA     + D +C  C++ +Q+I+ 
Sbjct: 98  LGEKDDTPVHFCDKCGLPIKMYGRMIPCKHVFCYDCAILHEKKGDKMCPGCNEPVQRIEQ 157

Query: 126 IKLMEGIFICA-APHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTA 185
             +   +F+C+    C +++L + + ++HI+  H    KP                    
Sbjct: 158 C-VRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKP-------------------- 217

Query: 186 SESTVRGPLRPLISPGSNSQPQERDEKF-----HRQQSRDQPRSGMQQKQTPSFGQNQNN 245
                R PL P + P     P E  E+F         S   P+  +     P       +
Sbjct: 218 ---VTRPPLEP-VHPPIAPPPAEIPERFIMPPEKHHMSHIPPKQHIMMPPPPLQHVPHEH 277

Query: 246 TSESQQDSGHSQGFDRHGPHGRFPPQNFDAQG--------------APHQDSSQFPEKQQ 305
            ++  +D           P    PP    +Q                P QD S    ++ 
Sbjct: 278 YNQPHEDIRPPPAEMSMAP----PPPRPVSQDTFRISTRKHSNLITVPIQDDSNSGAREP 337

Query: 306 GILSDTPYSQYPPLQPIP---PPNYVVPANSNPMLTPPLPFGYPPF--PIEGAQQYYNTP 365
              +  P   +P  Q  P    P++++P   +    PP P   PP   P++   Q   TP
Sbjct: 338 PPPAPAPAHHHPEYQGQPVVTHPHHIMPPQQHYAPPPPPP---PPISHPLQHPPQAAGTP 397

Query: 366 YEVSRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQSWNTAQAGIPYEHAGGGQGDYR 425
           + V  Q       S    +   PPG     +  A  P   N    G P    GG   +  
Sbjct: 398 HMVYSQAPPPPMTSAPPPITP-PPG-----HIIAQMPPYMNHPPPGPPPPQHGGPPVNVS 457

Query: 426 RSPGRMPVNSSAGNAMDIRDGKGILAP---QP------LIQLPPPPPPPPYM 435
             P   P + +  +     + +G L+P   QP      +   P  PPPPP M
Sbjct: 458 APP---PHHYNPNSLPQFSEDQGTLSPPFTQPGGMSPGIWPAPRGPPPPPRM 468

[Close]

BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot
Match: Q9JIY2 (E3 ubiquitin-protein ligase Hakai OS=Mus musculus OX=10090 GN=Cbll1 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-05
Identity = 102/413 (24.70%), Postives = 148/413 (35.84%), Query Frame = 0


Query: 66  LGER----VHFCVRCDFPIAIYGRLSPCEHAFCLDCA-----RSDSLCYLCDDRIQKIQT 125
           LGE+    VHFC +C  PI +YGR+ PC+H FC DCA     + D +C  C D +Q+I+ 
Sbjct: 98  LGEKDDTPVHFCDKCGLPIKVYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQ 157

Query: 126 IKLMEGIFICA-APHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTA 185
                 +F+C+    C +++L + + ++HI+  H    KP       N     +   +  
Sbjct: 158 C-TRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTDI 217

Query: 186 SESTVRGPLRPLIS------------PGSNSQPQERDEKFHR---------QQSRDQPRS 245
            +  +  P +  +S            P     P E   + H            +   PRS
Sbjct: 218 PDRFIMPPDKHHMSHIPPKQHIMMPPPPLQHVPHEHYNQPHEDIRAPPAELSMAPPPPRS 277

Query: 246 GMQQKQTPSFGQNQNNTSESQQDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEK 305
             Q+    S  ++ N  +   QD   S          R PP    A    H      PE 
Sbjct: 278 VSQETFRISTRKHSNLITVPIQDDSSS--------GAREPPPPAPAPAHHH------PEY 337

Query: 306 QQGILSDTPYSQYPPLQPIPPPNYVVPANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEV 365
           Q   +   P+   PP Q   PP    P  S+PM  PP              Q   TP+ V
Sbjct: 338 QGQPVVSHPHHIMPPQQHYAPPPPPPPPISHPMPHPP--------------QAAGTPHLV 397

Query: 366 SRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQSWNTAQAGIPYEHAGG----GQGDY 425
             Q       S    +   PPG     +  A  P   N    G P    GG        +
Sbjct: 398 YSQAPPPPMTSAPPPITP-PPG-----HIIAQMPPYMNHPPPGPPPPQHGGPPVTAPPPH 457

Query: 426 RRSPGRMPVNSSAGNAMDIRDGKGILAP---QP------LIQLPPPPPPPPYM 435
             +P  +P            + +G L+P   QP      +   P  PPPPP M
Sbjct: 458 HYNPNSLP---------QFTEDQGTLSPPFTQPGGMSPGIWPAPRGPPPPPRM 466

[Close]

BLAST of Cucsat.G12209 vs. NCBI nr
Match: XP_004137915.1 (E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >XP_031738067.1 E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >KGN58802.1 hypothetical protein Csa_000851 [Cucumis sativus])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209 vs. NCBI nr
Match: XP_008442473.1 (PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >XP_008442475.1 PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >KAA0044143.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa] >TYK24994.1 E3 ubiquitin-protein ligase Hakai [Cucumis melo var. makuwa])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 462/471 (98.09%), Postives = 466/471 (98.94%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AESGGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209 vs. NCBI nr
Match: XP_038905987.1 (E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida] >XP_038905988.1 E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida])

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 453/491 (92.26%), Postives = 461/491 (93.89%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AE GGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGN+IEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNDIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPL+SPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQTP+FG NQNNTSESQQDSGH
Sbjct: 181 RGPLRPLVSPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQTPNFGPNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQF EKQQGIL+DTPYSQYPPLQ IPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFSEKQQGILADTPYSQYPPLQSIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNP+LTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG
Sbjct: 301 PANSNPILTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPVN 420
           MNFSATYPQ WNT Q GIPYEHAGGGQG                    DYRRSPGRMPVN
Sbjct: 361 MNFSATYPQPWNTGQGGIPYEHAGGGQGMPDAFANTPDSQGKHGFYQGDYRRSPGRMPVN 420

Query: 421 SSAGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDS 471
           SS GNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKR KFYSGDMDHDGQSLGWQNDS
Sbjct: 421 SSVGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRVKFYSGDMDHDGQSLGWQNDS 480

[Close]

BLAST of Cucsat.G12209 vs. NCBI nr
Match: XP_023527487.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842 bits (2176), Expect = 6.66e-306
Identity = 429/506 (84.78%), Postives = 451/506 (89.13%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AE G GAVKP PAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQ+S QF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESGQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAAG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

[Close]

BLAST of Cucsat.G12209 vs. NCBI nr
Match: KAG6580495.1 (E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017247.1 E3 ubiquitin-protein ligase Hakai [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 837 bits (2161), Expect = 1.28e-303
Identity = 427/506 (84.39%), Postives = 449/506 (88.74%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AE G GAVKP PAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNY V
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYAV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAAG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHATGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PP ++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPAFLSHNKRGKFYPG 480

[Close]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL
Match: A0A0A0LDI2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G732590 PE=4 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL
Match: A0A1S3B5R9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486330 PE=4 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 462/471 (98.09%), Postives = 466/471 (98.94%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AESGGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL
Match: A0A5A7TKW6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001120 PE=4 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 462/471 (98.09%), Postives = 466/471 (98.94%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AESGGGAVKPLPAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAESGGGAVKPLPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPC+HAFCLDCARSDSLCYLCDDRIQKIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCDHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR+EFESHIHE+HADLLKPNADKEDGNEIEANSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEANSAKQSTASESTV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHG HGRFPPQNFDAQGAPHQDSSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGSHGRFPPQNFDAQGAPHQDSSQFLEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAAGG 360
           PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGA GG
Sbjct: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAAETGSEQGSLLGFPPGAPGG 360

Query: 361 MNFSATYPQSWNTAQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420
           MNFSATYPQSWNT QAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP
Sbjct: 361 MNFSATYPQSWNTGQAGIPYEHAGGGQGDYRRSPGRMPVNSSAGNAMDIRDGKGILAPQP 420

Query: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471
           LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD
Sbjct: 421 LIQLPPPPPPPPYMSHNKRGKFYSGDMDHDGQSLGWQNDSHSRDSFGSGQD 471

[Close]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL
Match: A0A6J1J5I8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 PE=4 SV=1)

HSP 1 Score: 835 bits (2156), Expect = 3.59e-303
Identity = 427/506 (84.39%), Postives = 449/506 (88.74%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AE G GAVKP PAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           R PLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQ NTSESQQDSGH
Sbjct: 181 RCPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQINTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAAG 360
           P NSNPMLTPPLPFGYPPFP+EGAQQYY+TPYEV RQDTAA ETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

[Close]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL
Match: A0A6J1F6T3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395 PE=4 SV=1)

HSP 1 Score: 834 bits (2155), Expect = 5.10e-303
Identity = 425/506 (83.99%), Postives = 449/506 (88.74%), Query Frame = 0


Query: 1   MLQIRLSKVPLAESGGGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVP AE G GAVKP PAE+VTVACPDHLVLADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKLM 120
           RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRSEFESHIHENHADLLKPNADKEDGNEIEANSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKR++FESHIHE+HADLLKPNA+KEDGNEIEA+SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGPLRPLISPGSNSQPQERDEKFHRQQSRDQPRSGMQQKQTPSFGQNQNNTSESQQDSGH 240
           RGPLRPLISPGSNSQPQER+EKFHRQQSRDQPRSGMQQKQ P  GQNQNN SESQQDSGH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQDSSQFPEKQQGILSDTPYSQYPPLQPIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFDAQGAPHQ+SSQF EKQQGILSDTPYSQYPPLQPIPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLTPPLPFGYPPFPIEGAQQYYNTPYEVSRQDTAA-ETGSEQGSLLGFPPGAAG 360
           P NSNPMLTPPLPFGYPPFP+EGAQ+YY++PYEV RQDTAA ETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQKYYSSPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYPQSWNTAQAGIPYEHAGGGQG--------------------DYRRSPGRMPV 420
           GMNFSATYP  WN  QAGIPY+HA GGQG                    DYRRSPG MPV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSS--------------AGNAMDIRDGKGILAPQPLIQLPPPPPPPPYMSHNKRGKFYSG 471
           NS+              +G+ MD+RDGKGILAPQPLIQLPPPP PPP++SHNKRGKFY G
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQPLIQLPPPPRPPPFLSHNKRGKFYPG 480

The following BLAST results are available for this feature:

BLAST of Cucsat.G12209 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFC0	1.4e-84	50.13	E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAK...	[more]
Q75N03	1.8e-07	26.86	E3 ubiquitin-protein ligase Hakai OS=Homo sapiens OX=9606 GN=CBLL1 PE=1 SV=1	[more]
Q4R7I8	1.8e-07	26.86	E3 ubiquitin-protein ligase Hakai OS=Macaca fascicularis OX=9541 GN=CBLL1 PE=2 S...	[more]
Q5ZHZ4	4.3e-06	23.79	E3 ubiquitin-protein ligase Hakai OS=Gallus gallus OX=9031 GN=CBLL1 PE=2 SV=1	[more]
Q9JIY2	3.7e-05	24.70	E3 ubiquitin-protein ligase Hakai OS=Mus musculus OX=10090 GN=Cbll1 PE=1 SV=1	[more]

BLAST of Cucsat.G12209 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137915.1	0.0	100.00	E3 ubiquitin-protein ligase HAKAI homolog [Cucumis sativus] >XP_031738067.1 E3 u...	[more]
XP_008442473.1	0.0	98.09	PREDICTED: E3 ubiquitin-protein ligase Hakai [Cucumis melo] >XP_008442475.1 PRED...	[more]
XP_038905987.1	0.0	92.26	E3 ubiquitin-protein ligase HAKAI homolog [Benincasa hispida] >XP_038905988.1 E3...	[more]
XP_023527487.1	6.66e-306	84.78	E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo]	[more]
KAG6580495.1	1.28e-303	84.39	E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma ...	[more]

BLAST of Cucsat.G12209 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDI2	0.0	100.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G732590 PE...	[more]
A0A1S3B5R9	0.0	98.09	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486330 PE=4 ...	[more]
A0A5A7TKW6	0.0	98.09	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1J5I8	3.59e-303	84.39	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 P...	[more]
A0A6J1F6T3	5.10e-303	83.99	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 70..124 e-value: 1.7E-20 score: 74.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 451..471
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 152..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 418..471
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 420..435
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 210..242
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 152..274
None	No IPR available	PANTHER	PTHR13480:SF0	E3 UBIQUITIN-PROTEIN LIGASE HAKAI	coord: 1..439
IPR040383	E3 ubiquitin-protein ligase HAKAI/CBLL2	PANTHER	PTHR13480	E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED	coord: 1..439
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 89..98
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 126..149
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 124..150 score: 8.600363
IPR040380	HAKAI-like, RING finger, HC subclass	CDD	cd16508	RING-HC_HAKAI_like	coord: 71..107 e-value: 1.08743E-18 score: 77.0322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G12209.T1	Cucsat.G12209.T1	mRNA
Cucsat.G12209.T2	Cucsat.G12209.T2	mRNA
Cucsat.G12209.T6	Cucsat.G12209.T6	mRNA
Cucsat.G12209.T7	Cucsat.G12209.T7	mRNA
Cucsat.G12209.T5	Cucsat.G12209.T5	mRNA
Cucsat.G12209.T3	Cucsat.G12209.T3	mRNA
Cucsat.G12209.T8	Cucsat.G12209.T8	mRNA
Cucsat.G12209.T10	Cucsat.G12209.T10	mRNA
Cucsat.G12209.T9	Cucsat.G12209.T9	mRNA
Cucsat.G12209.T4	Cucsat.G12209.T4	mRNA
Cucsat.G12209.T11	Cucsat.G12209.T11	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0030155	regulation of cell adhesion
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity