Cucsat.G11973.T8 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G11973.T8
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionEndoglucanase
Locationctg1820: 4176327 .. 4181542 (-)
RNA-Seq ExpressionCucsat.G11973.T8
SyntenyCucsat.G11973.T8
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCCAAGCTTATATAGTGCTCCCATCCTCACTTCCACTCCCCACCCCACACTCTCCTTCTTGTTCATTCTTCATTTCTCTCTTCCCCTCTTTAGGGGTTCTTCTCCTCTGTTTCATAATCGATTTATCACAACTTTTATCTTGATAAATCCCACCGGCATTGCCATTTCCTTCTCCCATTCCTTCAAAATGGCAAATCCCATTTCCCCCATTGCTCTCTTTATCCTACTTCTAGCCCCCTCTGCCCTAGCCGGCCATGATTACGGCCAAGCTCTCACCAAGAGCCTTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCCACAACCAGCGCGTCAATTGGCGAGCCAATTCCGGCCTCCAAGACGGCAAGGCTAGCGGGGTAAACCCTTTAAAACCCTGTTTGGTTACTCAGAAAATCGATCGGGAAACTTAATTTGGGGATTTTGTATGTTTGTTGTAGGTGGATCTGGTCGGAGGATACTACGACGCCGGTGACAATGTGAAGTTCGGACTTCCGATGGCGTTCACGGTGACGATGATGAGTTGGAGTATTATTGAATATGGAAAACAAATGGCCACTAATGGCGAACTTAGCCATGCCCTAGACGCCGTCAAATGGGGCACCGATTACTTCATCAAAGCCCACCCTGAACCCTATGTTCTCTATGGAGAGGTAACCTTAATCTTTTTCGTTCTTTTTAATTCAACGATTTCAAATTAATCCAACCATCAAACTTTGATATGATAAGTGGGTCAATTCAACGCAATTTTCTTTCATAACGTTAGATTTTATTAGGTAGAGATGTTTTAATTTGTACGTTGATTTGTTATGAAATGATAGTTCTATTAATGTTTTACTGACCATTATGCTTAACTAGTTAGAATAAAATTGGTAATTATACTTAAGGGAAGGACTCTAATATCTAATTTTTTTACTGATATTTGACTATTTTAGTAAATTAAACACCAATTTGTCGCCTTGTTCAAATGTCTTATGTTAGTTGTTAAATTTATTATTAAAATATAAGTGTGTGTATTTTAGGGGTGATTTGTCAATTGCATAAATGAGAGGGACTTAATTTGTAATTAGGCTTGATTTGTGTGTGTGTGTTTTTTTAGTTAAAATTATGAAAAAGGGATTAACAACATGGTTAATGAAATGGGATTTTAGGAAATTTCTGTTATTAAAATGAATAAATATTTGAAATTAACAAGTATTATCTTTTGAAATAAAAATCTGAGAATGCCAAAAAGTACGTTAGATTCCAAAAAAATGTGATTGGTTTAATTTGTTTTTGTAAAATCATCTTTGAGTGGAAAAATTAAAAGCAAATTGATGTACGGAATCATTAGGTGGGAGATGGGAACACGGACCATTACTGCTGGCAGCGGCCGGAGGACATGACGACGGACCGCCACGCTTACAAGATCGACCCAAGCAATCCCGGGTCGGATCTCGCCGGAGAAACCGCCGCCGCTATGGCCGCCGCCTCCATCGTCTTCCGCCACTACAACCCTTCCTATGCCAATGAGCTCCTTTCCCATGCTCGCCAGGTTAGCCATTTTTATCATCTTTTTTATTTTTATTTCCACCAATTATGTATTCTTTTTTATTTTTCTGAAATGTATAAAATCATCCACAATAAAAGAAAATGAAAATAATTTTACAATTTAAAGCGTAGGCTAGCGGTACCCCACAGCACATGATACTATTAACTACCGCAGTTAATAGGTTTATTTATTGTGATATTGAAAGCTGTTGTTTTTTTAATAAGAAACATTTATTGTGTAATAAAAAAATATATTTTTTTTCTCAATTGAAAAAAATATTTTAAAGAGAATGTTTAAGAAATGTTTGGGTAAATTATAAACTTAGTTTTAGTTGGAGATGAGTTATAATTATAGTTTAGTCTGTAGAGTTAATTTAACAAAAATATTTATAGTGAATAAAAATAGGATTTTTAATATTTTGTTGGAAAAAAATAGCATAAAAATAGGCATGTGATAAAACTGTCGGTGGGAACTGTTTGGGGATGTGCATGTAGATGTGAAGTTACGGGAATGCCATTGGAGAGATTTAATAAATTTGTTTATTAAAAATGTATTTTGTTTGTCAGCTGTTTGAATTTGCGGATAAATACAGAGGAAAGTACGACAGCAGCATCACTGTGGCTCAGAAGTATTATCGATCCGTTAGTGGATACAATGTGCGTAAGCGTATCATCCTTCCCCTTTTCTTGGGCCCTTCTTCTATGGAAGCCCGTTGCTTACTGATTATTACTCACCTATCCCTTTGTTATGGGTTATTTGCAACGTTACTCCTGGAAATGATGACAATAATTCTGTAATTATAATTTGCAGGATGAGTTATTGTGGGCAGCAGCGTGGCTGTATGAGGCAACAAACAACCAATATTACTTGAATTACTTGGGAGAAAATGGTGACTCCATGGGAGGAACTGGTTGGAGTATGACTGAATTTGGTTGGGATGTCAAATATGCTGGTGTCCAAACCCTTGTTGCCAAGGTTGGTTTCCATTTTATTTTTTACATTTCTTTTTATCAAAAAAAAAAAATTATATACATTTAGAACTTTATGGGTGATTTAGACCCGACTATAACTGGGTGGAGTGACCACTACTTATTTGGAGTTTAAACAATAATAGTTGGTGTGACCACTGCAATTTTCTATTTCAAACTCTCTTTATTACAATATTTATCGTTTCTTGTTTGTTACATTGTAAAGTCATTTAAGTACAAATTATTATAATTTATATACTATAATAACTGATTTAATTCTCTTAAACCCTTGTTATAGTTCAAAAGTTGGTAAGGAAGTGTTATATACTATAATAACTGATTTAATGCTCTTAAACCCTTGTTATAGTTCAAAAGTTGGTAAGGAAGTGCTCTGAAAAAGACACCTAATAAGTTCATAACTTTTCAATTCAACTTTGTGTTAAAAGAATCAAAAGTTTAATGAGCTATTGGCACACAAATTTAATTTATAGTAAATAAGTAAGTTTTGTTTGAGAGGGTTTGGTTCTTGCAATTTGTGGATTGTAGATTTATTAATTCTTAAAAAGTTGAATGTTGAGTTTGAAGTAATTTTGATGTTTGTGATCGCAGTTTTTATTGCAAGGAAGAGCGGCTCACCATGCGCCGGTGTTCGAGAGGTACAAGGAGAAGGCTGAAAATTTCTTATGTTCTTGCATGAAGAAAGGCACTCGCAATGTTCAGACCACACCGGCCGGTCTCATCTTCCGTCAGAGATGGAACAATATGCAGTTTGTTACCAGCGCTTCTTTCCTCGCCACCGTCTACTCAGACTACCTTTCTTCCTCTGGTGGAAACTTGAAGTGCGCCGCCGGTTATGTCCAGCCTTCCGAGCTTCTTCGCTTTGCACAATCCCAGGTCCCTACATTTTCTCTTCAAATGGGAACAACTAGTTTTTGATCTTCAAGGTCAATGTTTGTTAACTGTTCGACGAATTGCCTAAATGGGGTTTTGATTTTGAACCAGGTGGATTACATTCTTGGGGACAATCCAAGAGCTACTAGTTACATGGTGGGATATGGAAACAATTACCCACGACAAGTCCATCACAGGGGATCTTCCATTGTTTCAATCAAGGTCAATTCCGCCTTTGTGAGCTGCAGGGGTGGCTATGCAACATGGTACACTAGAAAGGCAAGTGACCCAAACCTACTCACTGGTGCTCTTGTGGGTGGACCTGATGCCTATGACAACTTTGCTGATCAAAGGGACAATTATGAGCAGACTGAGCCTGCTACTTATAACAATGCCCCAATTCTTGGCATTTTAGCCAGGCTCAGTGGTGGACATAGTGGCTACAATCAGCTCCTTCCAGGTACTTACCTCATTTTGATCATCAGATTTCTCTTAAAGCATTTTCAAGTGCAATCATGGCATTGAGCATCCTTTTGTTTTCTCTCCAGTGCTTCTTCCTCCAGTTAATCATCCAGACCCATCTGCACAATCCAAGACACTTCCATCTCCAGGTATGATGCCTTTGCAATTATTTGGTTGGCTTAGAATACTTTGAACAGATCATTTTCTATATCACATGAACTGTTTGGTACTTAAGATGTCCATCACAAAAGGTTCTTCATGCCAACCAATCCAATTGGATTTTTAAGAAATAGTTGATGAATGACTAAATTGTGGCATAAAGCTGGTTTAATTCAAGAAATAGAAACTATTAGGATGAATGACTAAATTGGTCATGGAAGGCGAAAATTTGACAGAAAAGCTTGTTTTTGCAGCATCGTCTTCTACCCCAATTACCGTAGAGCAAAAGATGACGACTTCCTGGACTGCTAAAGGAAATACCTACTATAGATACTCTACAAAAGTGACCAACAAGTCACCAAAAACTCTCAAAAACCTGAAACTTTCGATATCTCAACTTTATGGGCCTCTCTGGGGGCTGGAGAAATCTGGTGAATCATATGTCTTCCCATCTTGGCTTAACTCATTAGCTGCAGGAGAAAGCCTTGAATTTGTTTACATTCACACTGCTTCTCAAGCCAAAATCTCGGTTTCCAGCTACACTCTCTCCTAAATATGAGGGAAGAATCTTGGAAACCAAATCAAGGCAATACAGCATACAAGCTTATGCGGTTCATGGAGTGGTGTAAGAATTGTGCAGTTATAGATAGCGAGAAAGTCCATATTTACGCGTGATATGTATTTGCTTAGGTTTTTTTTAGATGCAATTAGGCCAAATAATGGTGCTGAAGTTTGGGGGAAGAAAGGAGAGAAGAGAGTTTGAAACGGTGCTCATCCTCTTTCTCCACCCTCTTCAATGCTTTATAGACAGAATGAACAGCTGAGAGTGTCAGAGAAGGCAAACAAAGATGGCTTCTCAAAGGATTTGCAAGAGAGTTCTCATTTCTTTCCTTGTATTTTGAATTAGCTTTATGTATCCTTTTAGATGTGTGATTTGCAACATTTTCTTGATTCACACTCGAATGTAGTTTGTGAAACATGGTGTGCTTTTGCTTATCTTGTTTTTAAGATATTGTAAATTCATCACTATACTCAAAACCTTTTGTGTTACTTTTATGGTAAAGTGATTTTAATGGTTTGAAATGTTCTTAAACCATTGGGACATGGCATTTGTACCTTGAAAACAATGAATGTCAATCAATACAACTACTTTTGAAGTTTTTTCATTCAATGTACAAT

Coding sequence (CDS)

ATGGGAGGAACTGGTTGGAGTATGACTGAATTTGGTTGGGATGTCAAATATGCTGGTGTCCAAACCCTTGTTGCCAAGTTTTTATTGCAAGGAAGAGCGGCTCACCATGCGCCGGTGTTCGAGAGGTACAAGGAGAAGGCTGAAAATTTCTTATGTTCTTGCATGAAGAAAGGCACTCGCAATGTTCAGACCACACCGGCCGGTCTCATCTTCCGTCAGAGATGGAACAATATGCAGTTTGTTACCAGCGCTTCTTTCCTCGCCACCGTCTACTCAGACTACCTTTCTTCCTCTGGTGGAAACTTGAAGTGCGCCGCCGGTTATGTCCAGCCTTCCGAGCTTCTTCGCTTTGCACAATCCCAGGTGGATTACATTCTTGGGGACAATCCAAGAGCTACTAGTTACATGGTGGGATATGGAAACAATTACCCACGACAAGTCCATCACAGGGGATCTTCCATTGTTTCAATCAAGGTCAATTCCGCCTTTGTGAGCTGCAGGGGTGGCTATGCAACATGGTACACTAGAAAGGCAAGTGACCCAAACCTACTCACTGGTGCTCTTGTGGGTGGACCTGATGCCTATGACAACTTTGCTGATCAAAGGGACAATTATGAGCAGACTGAGCCTGCTACTTATAACAATGCCCCAATTCTTGGCATTTTAGCCAGGCTCAGTGGTGGACATAGTGGCTACAATCAGCTCCTTCCAGTGCTTCTTCCTCCAGTTAATCATCCAGACCCATCTGCACAATCCAAGACACTTCCATCTCCAGCATCGTCTTCTACCCCAATTACCGTAGAGCAAAAGATGACGACTTCCTGGACTGCTAAAGGAAATACCTACTATAGATACTCTACAAAAGTGACCAACAAGTCACCAAAAACTCTCAAAAACCTGAAACTTTCGATATCTCAACTTTATGGGCCTCTCTGGGGGCTGGAGAAATCTGGTGAATCATATGTCTTCCCATCTTGGCTTAACTCATTAGCTGCAGGAGAAAGCCTTGAATTTGTTTACATTCACACTGCTTCTCAAGCCAAAATCTCGGTTTCCAGCTACACTCTCTCCTAA

Protein sequence

MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTRNVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASDPNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLLPPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNLKLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS
Homology
BLAST of Cucsat.G11973.T8 vs. ExPASy Swiss-Prot
Match: Q42059 (Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2)

HSP 1 Score: 537.0 bits (1382), Expect = 1.7e-151
Identity = 255/358 (71.23%), Postives = 306/358 (85.47%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFL+QG+A  HAPVF +Y+EKA++F+CS + K +R
Sbjct: 264 MGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKAGRHAPVFRKYQEKADSFMCSLLGKSSR 323

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           N+Q TP GLIFRQRWNNMQFVTSASFL TVYSDYL+SS  NL+CAAG V PS+LL FA+S
Sbjct: 324 NIQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDYLTSSRSNLRCAAGNVAPSQLLSFAKS 383

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNN+P++VHHRGSSIVS+KV+  FV+CRGGYATW++RK SD
Sbjct: 384 QVDYILGDNPRATSYMVGYGNNFPQRVHHRGSSIVSVKVDRTFVTCRGGYATWFSRKGSD 443

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVL- 240
           PNLLTGA+VGGPDAYDNFAD+RDNYEQTEPATYNNAP+LG+LARLS GHSGY+Q LPV+ 
Sbjct: 444 PNLLTGAIVGGPDAYDNFADRRDNYEQTEPATYNNAPLLGVLARLSSGHSGYSQFLPVVP 503

Query: 241 LPPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKN 300
            P V  P P  + K + +P  +S P+ + QK+T+SW +KG TYYRYST V NKS + LK+
Sbjct: 504 APVVRRPMPIRRPK-VTTPVRASGPVAIVQKITSSWVSKGRTYYRYSTTVINKSSRPLKS 563

Query: 301 LKLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 358
           L LSI  LYGP+WGL +SG S+  PSW++SL +G+SLEFVYIH+ + A ++VSSYTL+
Sbjct: 564 LNLSIKNLYGPIWGLSRSGNSFGLPSWMHSLPSGKSLEFVYIHSTTPANVAVSSYTLA 620

BLAST of Cucsat.G11973.T8 vs. ExPASy Swiss-Prot
Match: Q8L7I0 (Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.0e-144
Identity = 249/361 (68.98%), Postives = 296/361 (81.99%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAK L+QG+   H  VFERY++KAE F+CS + K T+
Sbjct: 265 MGGTGWSMTEFGWDVKYAGVQTLVAKVLMQGKGGEHTAVFERYQQKAEQFMCSLLGKSTK 324

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           N++ TP GLIFRQ WNNMQFVTSASFLATVYSDYLS S  +L C+ G + PS+LL F++S
Sbjct: 325 NIKKTPGGLIFRQSWNNMQFVTSASFLATVYSDYLSYSKRDLLCSQGNISPSQLLEFSKS 384

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYG NYPRQVHHRGSSIVS  V+  FV+CRGGYATW++RK SD
Sbjct: 385 QVDYILGDNPRATSYMVGYGENYPRQVHHRGSSIVSFNVDQKFVTCRGGYATWFSRKGSD 444

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PN+LTGALVGGPDAYDNFADQRDNYEQTEPATYNNAP+LG+LARL  G +G++QLLP + 
Sbjct: 445 PNVLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPLLGVLARLISGSTGFDQLLPGVS 504

Query: 241 PP----VNHPDPSAQSK-TLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPK 300
           P     +  P P  Q K T P  ASS +PIT+ QKMT SW  +G  YYRYST +TN+S K
Sbjct: 505 PTPSPVIIKPAPVPQRKPTKPPAASSPSPITISQKMTNSWKNEGKVYYRYSTILTNRSTK 564

Query: 301 TLKNLKLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYT 357
           TLK LK+SI++LYGP+WG+ K+G S+ FPSW+ SL +G+S+EFVYIH+AS A + VS+Y+
Sbjct: 565 TLKILKISITKLYGPIWGVTKTGNSFSFPSWMQSLPSGKSMEFVYIHSASPADVLVSNYS 624

BLAST of Cucsat.G11973.T8 vs. ExPASy Swiss-Prot
Match: Q6L4I2 (Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.4e-110
Identity = 207/364 (56.87%), Postives = 262/364 (71.98%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           +GGTGWS+ +FGWDVKY GVQ L AKFLLQGRA  HA   +RY++ AE F+CSC+ KG  
Sbjct: 273 LGGTGWSINQFGWDVKYPGVQVLAAKFLLQGRAGDHAAALQRYRQNAEFFVCSCVGKGAV 332

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSG-GNLKCAAGYVQPSELLRFAQ 120
           NV  TP G+++ QRWNN+QFVTSASFL TVY+D+ + SG G + C AG  QP ++L+F +
Sbjct: 333 NVARTPGGMMYHQRWNNLQFVTSASFLLTVYADFAAISGRGAVHCPAGAAQPFDILKFVK 392

Query: 121 SQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKAS 180
           SQV+YILGDNPR TSYMVGYG +YPRQVHHRG+SIVSIK + +FVSC+ GY++WY R+A 
Sbjct: 393 SQVNYILGDNPRGTSYMVGYGASYPRQVHHRGASIVSIKRDPSFVSCQEGYSSWYGREAG 452

Query: 181 DPNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSG-----YNQ 240
           +PNLL GA+VGGPD YD+FAD+RDNYEQTE ATYNNAP+LG+LARL+    G     Y Q
Sbjct: 453 NPNLLDGAVVGGPDEYDDFADERDNYEQTEAATYNNAPLLGVLARLAASCGGLKEEEYEQ 512

Query: 241 LLPVLLPPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS- 300
                 P VN    ++ S +LP+ A   T I +EQ +T +W  +  TYYRY+  VTN+S 
Sbjct: 513 --ETATPVVNR---TSSSSSLPATA---TAIGIEQNVTGTWARRRRTYYRYAVTVTNRSR 572

Query: 301 PKTLKNLKLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTA-SQAKISVS 357
            KT++ L L +S L G LWGLE++   YV P WL +L  G SL FVY+  A + A I V+
Sbjct: 573 GKTVRELHLGVSGLRGRLWGLEEARYGYVPPRWLPALRPGRSLRFVYVQPAPAPANIWVT 628

BLAST of Cucsat.G11973.T8 vs. ExPASy Swiss-Prot
Match: Q5NAT0 (Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-108
Identity = 204/366 (55.74%), Postives = 259/366 (70.77%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCM-KKGT 60
           +GGTGWS+ +FGWDVKY GVQ L AKFLLQG+A  HA V + Y+ KA+ F CSC+ K   
Sbjct: 279 LGGTGWSINQFGWDVKYPGVQILAAKFLLQGKAGEHAGVLQGYRRKADFFACSCLGKDAA 338

Query: 61  RNVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG---YVQPSELLR 120
            NV  TP G+++ QRWNN+QFVTSASFL  VYSD+L  +GG ++C+ G       +ELL 
Sbjct: 339 DNVGRTPGGMLYHQRWNNIQFVTSASFLLAVYSDHL--AGGAVRCSGGGGAVAGAAELLA 398

Query: 121 FAQSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTR 180
           FA+SQVDYILG NPR TSYMVGYG  YPRQ HHRGSSI SI+ + +FVSCR GYA+WY R
Sbjct: 399 FAKSQVDYILGSNPRGTSYMVGYGAVYPRQAHHRGSSIASIRASPSFVSCREGYASWYGR 458

Query: 181 KASDPNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLL 240
           +  +PNLL GA+VGGPD +D+FAD+R+NYEQTE ATYNNAP++GILARL+ GH    +  
Sbjct: 459 RGGNPNLLDGAVVGGPDEHDDFADERNNYEQTEAATYNNAPLMGILARLAAGHGA--RAR 518

Query: 241 PVLLPPVNHPDPSAQSKTLPSPAS--SSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSP 300
             L   + H   +A   +LP  A+   ++P+ +EQK T SW   G TY+RY+  V+N+SP
Sbjct: 519 GRLGQSLQH-GIAANHTSLPHGANHQHASPVEIEQKATASWEKDGRTYHRYAVTVSNRSP 578

Query: 301 ---KTLKNLKLSISQLYGPLWGLEKSGE-SYVFPSWLNSLAAGESLEFVYIHTASQAKIS 357
              KT++ L + I +LYGP+WGLEK+    YV PSW  SL AGES  FVY+H A  A + 
Sbjct: 579 AGGKTVEELHIGIGKLYGPVWGLEKAARYGYVLPSWTPSLPAGESAAFVYVHAAPPADVW 638

BLAST of Cucsat.G11973.T8 vs. ExPASy Swiss-Prot
Match: Q5NAT8 (Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.9e-97
Identity = 187/352 (53.12%), Postives = 241/352 (68.47%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKK--G 60
           +GGTGW+  EFGWDVKY GVQ L AKFLLQG+A  HA V  RY+  A+ F CSC+ K  G
Sbjct: 280 LGGTGWATNEFGWDVKYPGVQVLAAKFLLQGKAGPHAAVLRRYQRNADVFACSCLGKGGG 339

Query: 61  TRNVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGN---LKCAAG-YVQPSEL 120
             NV  TP GL++ Q WNN+QFVT ASFL  VY+D+L+++G     ++C AG   + SEL
Sbjct: 340 GGNVGRTPGGLMYHQGWNNLQFVTGASFLLAVYADHLAAAGRGQAVVRCQAGPAARASEL 399

Query: 121 LRFAQSQVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWY 180
           +  A+SQVDYILG NPR  SYMVGYG  YPR+ HHRG+SIVSI+ N +FVSC+ GYA+W+
Sbjct: 400 VALAKSQVDYILGSNPRGISYMVGYGARYPRRAHHRGASIVSIRANPSFVSCKDGYASWF 459

Query: 181 TRKASDPNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQ 240
            R  S+PNLL GA+VGGPD  D FAD+R+NY+QTE ATYNNAP++G+LARL+GG  G   
Sbjct: 460 GRAGSNPNLLDGAVVGGPDGRDGFADERNNYQQTEVATYNNAPLMGVLARLAGGGRG--- 519

Query: 241 LLPVLLPPVNHPDPSAQSKTLPSPASSSTPITVEQ-KMTTSWTAKGNTYYRYSTKVTNKS 300
              +    +  PD       LP  A++++P+ + Q   T SW   G TY RY+  V+N+S
Sbjct: 520 --GLAEAAIKRPD---NQTLLPPLAAAASPVEITQLNATASWKKDGRTYRRYAATVSNRS 579

Query: 301 P---KTLKNLKLSISQLYGPLWGLEKSGE-SYVFPSWLNSLAAGESLEFVYI 342
           P   KT++ L + I + +GP+WGLEK+    YV PS   SLAAGES  F Y+
Sbjct: 580 PAGGKTVEELHIGIGKPHGPVWGLEKAARYGYVLPS---SLAAGESAAFAYV 620

BLAST of Cucsat.G11973.T8 vs. NCBI nr
Match: XP_004149748.1 (endoglucanase 6 [Cucumis sativus] >KGN53401.1 hypothetical protein Csa_014642 [Cucumis sativus])

HSP 1 Score: 719 bits (1857), Expect = 1.55e-257
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. NCBI nr
Match: KAA0044906.1 (endoglucanase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 704 bits (1818), Expect = 1.32e-251
Identity = 348/357 (97.48%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG VQPSEL+RFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGNVQPSELIRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAP+LGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPLLGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSAKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTAS AK+SVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASPAKVSVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. NCBI nr
Match: XP_008451938.1 (PREDICTED: endoglucanase 6-like [Cucumis melo] >TYK16564.1 endoglucanase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 703 bits (1814), Expect = 5.36e-251
Identity = 347/357 (97.20%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVK+AGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKFAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG VQPSEL+RFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGNVQPSELIRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAP+LGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPLLGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSAKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTAS AK+SVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASPAKVSVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. NCBI nr
Match: XP_038893552.1 (endoglucanase 6 [Benincasa hispida])

HSP 1 Score: 697 bits (1800), Expect = 7.21e-249
Identity = 344/357 (96.36%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNL+CAAGYVQPSELLRFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLRCAAGYVQPSELLRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRK SD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKESD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHP PSAQ KTLPSP SSSTPIT+EQKMTTSWTAKGNTYYRYSTKVTNKSPKTLK+L
Sbjct: 503 PPVNHPVPSAQPKTLPSPVSSSTPITIEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKDL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWG+EKSGESYVFPSWL+SLAAGESLEFVYIHTAS A ISVSSYTLS
Sbjct: 563 KLSISQLYGPLWGMEKSGESYVFPSWLSSLAAGESLEFVYIHTASPANISVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. NCBI nr
Match: KAG6577144.1 (Endoglucanase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015144.1 Endoglucanase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 684 bits (1764), Expect = 2.15e-243
Identity = 337/357 (94.40%), Postives = 347/357 (97.20%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHH PVFERYK+KAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHGPVFERYKDKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSA+FLATVYSDYLSS+G NL+CAAG VQPSELLRFAQS
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSAAFLATVYSDYLSSAGANLRCAAGSVQPSELLRFAQS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWY RKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYGRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PP+NHPDPSA  KTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLK+L
Sbjct: 503 PPLNHPDPSAYPKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSSKTLKDL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KL+IS+LYGPLWGLEKSGESYVFP+WLNSLAAGESLEFVYIHTAS A ISVSSYTLS
Sbjct: 563 KLTISKLYGPLWGLEKSGESYVFPTWLNSLAAGESLEFVYIHTASPANISVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. ExPASy TrEMBL
Match: A0A0A0KZU5 (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_4G051530 PE=3 SV=1)

HSP 1 Score: 719 bits (1857), Expect = 7.49e-258
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. ExPASy TrEMBL
Match: A0A5A7TRY4 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002000 PE=3 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 6.40e-252
Identity = 348/357 (97.48%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG VQPSEL+RFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGNVQPSELIRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAP+LGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPLLGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSAKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTAS AK+SVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASPAKVSVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. ExPASy TrEMBL
Match: A0A5D3D018 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003840 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 2.60e-251
Identity = 347/357 (97.20%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVK+AGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKFAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG VQPSEL+RFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGNVQPSELIRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAP+LGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPLLGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSAKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTAS AK+SVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASPAKVSVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. ExPASy TrEMBL
Match: A0A1S3BS30 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103493091 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 2.60e-251
Identity = 347/357 (97.20%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVK+AGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKFAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAG VQPSEL+RFA+S
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGNVQPSELIRFAKS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAP+LGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPLLGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PPVNHPDPSAQSKTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLKNL
Sbjct: 503 PPVNHPDPSAQSKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSAKTLKNL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTAS AK+SVSSYTLS
Sbjct: 563 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASPAKVSVSSYTLS 619

BLAST of Cucsat.G11973.T8 vs. ExPASy TrEMBL
Match: A0A6J1EY30 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111437523 PE=3 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 5.98e-243
Identity = 336/357 (94.12%), Postives = 345/357 (96.64%), Query Frame = 0

Query: 1   MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHAPVFERYKEKAENFLCSCMKKGTR 60
           MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHH PVFERYK+KAENFLCSCMKKGTR
Sbjct: 263 MGGTGWSMTEFGWDVKYAGVQTLVAKFLLQGRAAHHGPVFERYKDKAENFLCSCMKKGTR 322

Query: 61  NVQTTPAGLIFRQRWNNMQFVTSASFLATVYSDYLSSSGGNLKCAAGYVQPSELLRFAQS 120
           NVQTTPAGLIFRQRWNNMQFVTSA+FLATVYSDYLSS+G NL+CAAG VQPSELLRFAQS
Sbjct: 323 NVQTTPAGLIFRQRWNNMQFVTSAAFLATVYSDYLSSAGANLRCAAGSVQPSELLRFAQS 382

Query: 121 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYTRKASD 180
           QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWY RK SD
Sbjct: 383 QVDYILGDNPRATSYMVGYGNNYPRQVHHRGSSIVSIKVNSAFVSCRGGYATWYGRKGSD 442

Query: 181 PNLLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 240
           PNLLTGALVGGPDAYDNFAD+RDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL
Sbjct: 443 PNLLTGALVGGPDAYDNFADERDNYEQTEPATYNNAPILGILARLSGGHSGYNQLLPVLL 502

Query: 241 PPVNHPDPSAQSKTLPSPASSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSPKTLKNL 300
           PP+NHPDPSA  KTLPSP SSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKS KTLK+L
Sbjct: 503 PPLNHPDPSAYPKTLPSPVSSSTPITVEQKMTTSWTAKGNTYYRYSTKVTNKSSKTLKDL 562

Query: 301 KLSISQLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTASQAKISVSSYTLS 357
           KL+IS+LYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHT S A ISVSSYTLS
Sbjct: 563 KLTISKLYGPLWGLEKSGESYVFPSWLNSLAAGESLEFVYIHTTSPANISVSSYTLS 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q420591.7e-15171.23Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2[more]
Q8L7I02.0e-14468.98Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1[more]
Q6L4I21.4e-11056.87Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 S... [more]
Q5NAT01.3e-10855.74Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1[more]
Q5NAT86.9e-9753.13Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149748.11.55e-257100.00endoglucanase 6 [Cucumis sativus] >KGN53401.1 hypothetical protein Csa_014642 [C... [more]
KAA0044906.11.32e-25197.48endoglucanase 6-like [Cucumis melo var. makuwa][more]
XP_008451938.15.36e-25197.20PREDICTED: endoglucanase 6-like [Cucumis melo] >TYK16564.1 endoglucanase 6-like ... [more]
XP_038893552.17.21e-24996.36endoglucanase 6 [Benincasa hispida][more]
KAG6577144.12.15e-24394.40Endoglucanase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015144.1 E... [more]
Match NameE-valueIdentityDescription
A0A0A0KZU57.49e-258100.00Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_4G051530 PE=3 SV=1[more]
A0A5A7TRY46.40e-25297.48Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002000 ... [more]
A0A5D3D0182.60e-25197.20Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003840 ... [more]
A0A1S3BS302.60e-25197.20Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103493091 PE=3 SV=1[more]
A0A6J1EY305.98e-24394.12Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111437523 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019028Carbohydrate binding domain CBM49SMARTSM01063CBM49_2coord: 265..345
e-value: 4.5E-24
score: 96.0
IPR019028Carbohydrate binding domain CBM49PFAMPF09478CBM49coord: 265..342
e-value: 9.2E-27
score: 93.2
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 9..222
e-value: 4.0E-52
score: 178.1
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 2..237
e-value: 1.2E-62
score: 214.5
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 1..322
NoneNo IPR availablePANTHERPTHR22298:SF157ENDOGLUCANASEcoord: 1..322
IPR033126Glycosyl hydrolases family 9, Asp/Glu active sitesPROSITEPS00698GH9_3coord: 198..216
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 124..150
IPR008965CBM2/CBM3, carbohydrate-binding domain superfamilySUPERFAMILY49384Carbohydrate-binding domaincoord: 262..353
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 5..226

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G11973Cucsat.G11973gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G11973.T8.E1Cucsat.G11973.T8.E1exon
Cucsat.G11973.T8.E2Cucsat.G11973.T8.E2exon
Cucsat.G11973.T8.E3Cucsat.G11973.T8.E3exon
Cucsat.G11973.T8.E4Cucsat.G11973.T8.E4exon
Cucsat.G11973.T8.E5Cucsat.G11973.T8.E5exon
Cucsat.G11973.T8.E6Cucsat.G11973.T8.E6exon
Cucsat.G11973.T8.E7Cucsat.G11973.T8.E7exon
Cucsat.G11973.T8.E8Cucsat.G11973.T8.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G11973.T8.C5Cucsat.G11973.T8.C5CDS
Cucsat.G11973.T8.C4Cucsat.G11973.T8.C4CDS
Cucsat.G11973.T8.C3Cucsat.G11973.T8.C3CDS
Cucsat.G11973.T8.C2Cucsat.G11973.T8.C2CDS
Cucsat.G11973.T8.C1Cucsat.G11973.T8.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G11973.T8Cucsat.G11973.T8-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0030245 cellulose catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0008810 cellulase activity