Cucsat.G10036.T13 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G10036.T13
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProlyl endopeptidase
Locationctg1673: 3679742 .. 3684908 (+)
RNA-Seq ExpressionCucsat.G10036.T13
SyntenyCucsat.G10036.T13
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGAGAAAAATTGTGATATTTAGAACAGTTTGAGAAGAAACCGTTGACTGAAATGACGCCTTTGCAACAAAACGGCATATTTGGAATACTTAGAAGAAGCTTCGTTCTCTTTATTCCCGTACTCTACCTTTCGCCGGTACTTCCGTCGGCAGCTTCATTCCGTCATTTCCGATCACCGGTTGCCACAATGAATCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGCATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCGAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATCGCTATACCGATTCCGTCATGTCCGGTGAACTCTAGTTTACATTTTCATTTCGGTTCTTGAGTTTTTTTTTTTGGTTACTACGTGAGAAATTTATGAATCAGTGATATGGTAGCGTTGATTTGATTTGAATGAGAGTAACGTCGTTTGATTTATCGATTGCTGATGATTTGTGTGTAATCTGGATTTTTTCTTTATTAAACGATATAGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTATTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATACTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCTAATGCTCCTCCGGAGCATGTTATACTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTATTGTAAAGACGGAACTGCTTCAGAATTTGATCACGTTTTCTCTTGAACAAAATTTTGATTCAAGGATCTTTATCGTCGTTTCTTCTCCAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGGGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGGGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGTGATTTACTGATTTTCTACCCTACATTCTGTACTTCTCATGGCTTTGACTGTTGTTTTCTCATTGGAAAAACAATTACTATCATGGGGTCTATAATCAAATAGGTCTCTTCATTTGTGGAATTAAAATATGAAGAAGACCAAATAAGTAAAAATCAATTTTAATCGAGGAGGAAATAATATTGCAAGGTCTTGAAGAGGACCTCCTAGACCATCCCGTCGATATCTGCATATGTCATCAGTTATTGTTGATTGTTCTGTGCATTCAGAATCGCCTTCTAATGATATTGTAGGTTGTTTCAGTAAAATGAAAATTCCTTACATACCATTAAATAGCTTGCATTCCTCTAGTTTTTCATTTGTAGTACTGATATATAGATTATAGTATCCTTATAATTTGATATCAGAAGAGTGGAGGCAGAGGAGTTTTTAGAGTTGTAAAGAGCGCTGCTCGAAGTACAAATTTTTGGTGAAAATACATTCAGTATCTGCATTCTGATGTTCCCAGTAATATTATTGTCGAACTTCAAATTGATAATTTCTTTTCAACGTTTTCAGAATTTTAGATGTTGATCTAGTAAATATTTTCCTCTTTCAATAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAGTATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCTTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAGGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGGTAGTGTTTCGAATTCTTTTATGATAATTGATGATTGACCTGATGGTATTATGTGAAGTTGAAACCCAATTGAAAGTGATGAGCTGATAGTTAATGACTTGAATCAACGAGCTTTAAAAGAAATCTTTGTCGGAACTTGGTGTTTATTTATTTAATTTGTTTGAAATATGTTAGGGTAGCTATTTTTATAAAATGAAAGACCCAACTGATCTATATCTAGGAGTGTAAGAAAAGAAAAGACAAGCTGAACTATAGCAAGGATTTTCATGTTCATTCGCTCTGACGTTTGGTACGTTGTTGGATTTATTCAACAAATTTAAATCTTATTGTGCTGTGAATCTGATTTTTTCTATACGAAATGTTAGAAGTTTCTTGATTAGCTAGTTATTGGGGCTTATGAAGTTGTTTGAATACTGTTCAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTACCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCGACTTATTCAGTGGATGCTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTAAGTAGTTCCACAATCATGGTTATGTTGTTCTCTTATGACCGGGATTGCCTCTAGGTTTAAATGAATTATTGTTAACCTTTAAATGTGATATTTTCAAATTTCCTGGTCATATTAGAAAAATATATATAAACCAGATATGAATTTCTATTCTCTGTTTTCTCAACCTAGTACTACCCATTTGTGGCTAGGAGTATATTTCATTTATGTATCTAGTCTTTCTAAAACCAGTGCTAACTTCAACTTTGAGTACATCCTTTTGCTTCCTTTGTTTCTTTTTCTTTCACTTCTTACGATTAAGAATTTTTATTCCCTATATGCTAGTGATGGTTTAGAAAAATCTTCCGCCTGTCTCTTAGTTGATTTTATGCCTTTTCATCGTCCGTTTTTTGTTGTTGTTGTTGAGAATTCTTGTTGTCTAGCGAATATAAAAAGTCTCCTCTTTTCATCGTCCGTTTTTTGTTGTTGTTGTTGTTGAGAATTCTTGTTGTCTAGCGAATATAAAAAGTCTCCTCCTTACCTTTCCTTCCCCCCAGGAGGATTTGAATTAATTCATCTTACTGTAACGTAGTGAAATTTCCACAAATTTTCTCAGGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGGTAACAAGTCTTGTGTTAATTAGACTCTATACGGATCTCAGGAACTCGAAGTTATTTTAAATTGAAAGAGTGTTTTTGTTTCCTGGGGACTACTCCCCCTATGAGTCAGAGTTTCTTTAATGGAGCTTCAAATAACGTGATTATAAATTTATAAGTTATTTTCCTGTTTTAATTGATTTATCAAAATTTCCAATTCTGAAATATTTATTTCTGCATTGTAATGGATTCTTTGAGGATCTATGTTTCGTTTTGATGTCCAGTGTTTTTGCTATAATTACGATCTCTGCGTTGCTAATTTACAGTTCTTAAACGTTTGAGGACATGAACTTGTACCTGAATCTTTTTGTTTACTTTTTTCTTGTCACTTAATTGAGTTTTGAATTGTAACAATGATGGTACATGAACCTGATCTCGAAGTGAATTTCATTCTTTGAACAGTATTGCATAGACCCTAGTTTCAAGGCATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACGGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGACGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGTAAATCTTCCCTAGCTAGTGTACCTTTCGCAATTCTGTAGGTGACATGCAGTGAATTAGTGCAATTCCTTGGTCAGTACTTAAATAGACAAGTTAAGGACTCTTTAACTTTAGCGGCATTGGCTCAAAATCACTTTTGAGTCCCCAAATTATGGTGCCATTTTGAACTAGAGTCCAATGTAGGATAGGCAGTTATCAGTTTAACAATATTATGTATTGAAAAAAACAAAAGGGTGGAAAGTGTCCCTGTTTGTAGCACGGTTCCTAACATACGAGATTTTCTTTTGACTCTGTAAATATCTTTACTTATCCATTATCTTCTTTTTTCACTTTTTTCAACTGTCGTTTACTGAAAATATGTTGTATAACTAATTGCAGCGCCTATTCTTTTTGACTTGCCACTTACCTTCTTTTGAATTCTGCAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGACAATGTACGCACCTCACTATTTTTTAATTAACATATTCTGTTTCAGCCATTTCCATCTAATCAATCATCCCCCTTGATCGCAACTTGATTTTGGATCATTTGACTGTGTCCGTGCTTATGTGTTTCATAATTCATAGAATATTTTCAAACTTTTGTTTTGATTAAGACATGACTTTATCTTAATGTTTCAGGTTAAGGCTCAAAATTATCCGGACGTACTTATTACTGCCGGCTTAAACGGTAAGTGTGATGGCTTAAAACTTCAATTCGAGTAGAGCCCCAATCAATTTTCCTGTTTCTTTATCCCGTGCACACATTCATTCTTCATATCCAGAATAGACA

Coding sequence (CDS)

ATGACGCCTTTGCAACAAAACGGCATATTTGGAATACTTAGAAGAAGCTTCGTTCTCTTTATTCCCGTACTCTACCTTTCGCCGGTACTTCCGTCGGCAGCTTCATTCCGTCATTTCCGATCACCGGTTGCCACAATGAATCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGCATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCGAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATCGCTATACCGATTCCGTCATGTCCGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTATTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATACTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCTAATGCTCCTCCGGAGCATGTTATACTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGGGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGGGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAGTATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCTTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAGGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTACCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCGACTTATTCAGTGGATGCTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGTATTGCATAGACCCTAGTTTCAAGGCATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACGGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGACGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGACAATGTTAAGGCTCAAAATTATCCGGACGTACTTATTACTGCCGGCTTAAACGGTAAGTGTGATGGCTTAAAACTTCAATTCGAGTAG

Protein sequence

MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLEGGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLNGKCDGLKLQFE
Homology
BLAST of Cucsat.G10036.T13 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 4.2e-130
Identity = 259/650 (39.85%), Postives = 382/650 (58.77%), Query Frame = 0

Query: 49  SHSPPVANKVEHKMEL-FGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKV 108
           S +PP   K  H ++   G  R D YYWLRDD R N ++L+YL  EN YTD+VM+  K +
Sbjct: 25  SATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYTDAVMAPLKPL 84

Query: 109 EDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTM 168
           ED+++ E+  RIK+DD +VP R+  ++YY R + GK+Y  + RR    G  +A+S+    
Sbjct: 85  EDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPG-VDAVSIQAAN 144

Query: 169 PTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAET 228
             G  A  E V+LD N     + YY++G +EVS +N+L+AYA+DT G   YT+   + +T
Sbjct: 145 AAGDFA-GEQVLLDVNALGAGKDYYNVGDYEVSQDNRLLAYADDTNGRRQYTIRFKNLDT 204

Query: 229 GAPVGKPLVGVTSYLKWAGDD-ALVYITMD-EILRPDKAWLHKLGTEQSMDTCLYHEKDD 288
           G  +   +      L W+ D   L Y+  D E L   +   H LGT  S D  +Y E+DD
Sbjct: 205 GELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQDALVYEEEDD 264

Query: 289 MFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHF 348
            F + +  S   K++ I+ ES  +        + P     VL PR   ++    H G+ +
Sbjct: 265 SFYMGIGRSRDDKFICISVESTVSSEMRCTPAASP-GVFTVLAPRERDVEYQADHLGDRW 324

Query: 349 FILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVILEREDGLPK 408
            I   ++   N ++V  P D+TS       + HR+ V ++  ELF    V+ ER + L  
Sbjct: 325 VIRTNADGATNFKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGFSVVAERANAL-- 384

Query: 409 VVVYSLPDIGEPLKTLEGGRAVDFADATYSVDAL----ESEFSSSILRFCYSSMKTPPST 468
                     E L+ ++   + D+  A  S  ++      E  +  LR+ Y+SM TP +T
Sbjct: 385 ----------ESLRVIKADGSSDYVKADESAYSMGLSANPETGTDWLRYSYTSMTTPATT 444

Query: 469 YDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDG-TKVPLSIVYRKDLVKLDGSD 528
           Y+ + KTG     K + V  G+D++KYVTER WA A DG TK+P+++VYRKD+ + DG  
Sbjct: 445 YEINTKTGERRQLKQQPV-PGYDASKYVTERVWAPARDGKTKIPVTLVYRKDVAR-DGKA 504

Query: 529 PLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTF 588
           P+L Y YGSY   +DP+F  + +SLLDRG +Y +AHIRGG EMGR WY++GKL  K NTF
Sbjct: 505 PMLQYAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMGRAWYDDGKLYNKINTF 564

Query: 589 TDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTM 648
           TDFI   +YL++  Y +K+++   G SAGGLL+GAV NM P+ +K  +  VPFVDV+TTM
Sbjct: 565 TDFIDVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVDVVTTM 624

Query: 649 LDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGL 689
           LDPTIPLTT+E++EWG+P ++ +Y Y+ +YSP DN++A+ YP + +  GL
Sbjct: 625 LDPTIPLTTNEYDEWGNPEEKGYYDYILTYSPYDNLQAKAYPAMFVGTGL 657

BLAST of Cucsat.G10036.T13 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.1e-127
Identity = 246/641 (38.38%), Postives = 377/641 (58.81%), Query Frame = 0

Query: 53  PVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIF 112
           P+A ++ H  EL GDVR D+YYWL+D  R N +V+ YL EENRY   +M   ++  +QI+
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKD--RDNTEVIQYLEEENRYYHEIMRPLQEQTEQIY 63

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
             +  R+ + ++ VP + G ++YY R  + K+Y  Y R+   +    A+    T      
Sbjct: 64  ESMVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARK---QAASRALLQDAT------ 123

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
              E V+LD N  AE   Y S+    ++ ++  +AY E+  G + YT+YI D  TG  + 
Sbjct: 124 ---EEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLS 183

Query: 233 K--PLVGVTSYLKWAG-DDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSL 292
              P V +   ++W    D + Y T+DE  RP + W H+LG++   D  ++ EKDD F+L
Sbjct: 184 DRVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTL 243

Query: 293 DLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILR 352
            +  S+S K++F+ S SK T     +D   P   L ++  R DGI     H  +   IL 
Sbjct: 244 FISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLIL- 303

Query: 353 RSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSL 412
            +E   N +++ CPL++ S+   ++ + E   +QE+  F + ++I  RE+GL ++ V   
Sbjct: 304 TNEGALNFQLLRCPLNDLSSKVNVVEYNEERYLQEMYPFRDKLLIAGRENGLTQIWVVHD 363

Query: 413 PDIGEPLKTLEGGRAVDFADATYSVDAL-ESEFSSSILRFCYSSMKTPPSTYDYDMKTGV 472
            ++          + + + +  Y+V  L E  + ++ +   Y S+ TP +T+  +++TG 
Sbjct: 364 GEL----------QQISWDEPLYTVAVLSEQSYDTNEVLIQYESLLTPKTTFGLNLQTGE 423

Query: 473 SVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSY 532
               +V  V G +D +++  E+ WAT   G KVP++ VY +  +  +G  PL+LYGYGSY
Sbjct: 424 KQCLQVAPVSGEYDRSQFRQEQLWATGRSGVKVPMTAVYLEGALD-NGPAPLILYGYGSY 483

Query: 533 EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYL 592
               DP F   R+ LL++G ++V A +RGG EMGR WYE+GK+  K+NTFTDFI++A++L
Sbjct: 484 GSNSDPRFDPYRLPLLEKGIVFVTAQVRGGSEMGRGWYEDGKMQNKRNTFTDFIAAAKHL 543

Query: 593 IENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTS 652
           I+  Y S  K+   G SAGGLL+GAV NM  +LFK  +  VPFVDV+TTMLD +IPLTT 
Sbjct: 544 IDQNYTSPTKMAARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTL 603

Query: 653 EWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLN 690
           EW+EWGDPRK+E YFYMKSYSP DNV+A++YP + IT G+N
Sbjct: 604 EWDEWGDPRKQEDYFYMKSYSPYDNVEAKDYPHMYITTGIN 618

BLAST of Cucsat.G10036.T13 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 431.0 bits (1107), Expect = 2.5e-119
Identity = 250/640 (39.06%), Postives = 351/640 (54.84%), Query Frame = 0

Query: 53  PVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIF 112
           P A ++ H M L GD RIDNYYWLRDD+RS P+VL YL++EN Y   VM+  + ++D+I 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
            EI  RI + +++ P  K  Y Y      G EY  Y R+     E +             
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWD------------- 122

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
                 +LD N +A +  +YS+G   ++P+N ++A AED      Y +   + ETG    
Sbjct: 123 --EWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYP 182

Query: 233 KPLVGVTSYLKWAGDDALVYITMDE--ILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLD 292
           + L  V     WA D  + Y        L P + W H +GT  S D  +Y EKDD + + 
Sbjct: 183 ELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVS 242

Query: 293 LEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRR 352
           L  + SK Y+ I   S  T     LD    +    V  PR    + Y      H F LR 
Sbjct: 243 LHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHE-YSLDHYQHRFYLRS 302

Query: 353 SEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLP 412
           +    N  +    + +      ++P RE++ ++   LF + +V+ ER+ GL      SL 
Sbjct: 303 NRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLT-----SLR 362

Query: 413 DIGEPLKTLEGGRAVDFADATY-SVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVS 472
            I    + + G   + F D  Y +  A   E  ++ LR+ YSSM TP + ++ DM TG  
Sbjct: 363 QINRKTREVIG---IAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGER 422

Query: 473 VLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE 532
            + K +T + GF +  Y +E  W  A DG +VP+S+VY +   +  G +PLL+YGYGSY 
Sbjct: 423 RVLK-QTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFR-KGHNPLLVYGYGSYG 482

Query: 533 YCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLI 592
             ID  F  SR+SLLDRGF+Y I H+RGGGE+G+QWYE+GK LKKKNTF D++ + + L+
Sbjct: 483 ASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALL 542

Query: 593 ENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSE 652
           +  Y S       G SAGG+L+G  IN RP+LF   IA VPFVDV+TTMLD +IPLTT E
Sbjct: 543 KLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGE 602

Query: 653 WEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLN 690
           +EEWG+P+  ++Y YMKSYSP DNV AQ YP +L+T GL+
Sbjct: 603 FEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLH 616

BLAST of Cucsat.G10036.T13 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.8e-80
Identity = 207/664 (31.17%), Postives = 322/664 (48.49%), Query Frame = 0

Query: 47  NQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKK 106
           N+S  PP+         L  DV ID+Y WLRD  R +PDVL+YL  EN Y D V S   +
Sbjct: 3   NKSLQPPLPRSERRIRVLHNDVTIDSYGWLRD--REDPDVLAYLEAENHYADEVTSYVAE 62

Query: 107 VEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDT 166
           ++  + +EI  R   D    P + G ++Y++++  G  +  + RR V  G          
Sbjct: 63  LKADLIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSGLLHSAWWRRPVTGG---------- 122

Query: 167 MPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAE 226
                   PE ++ D N     + +YS+G  E S + + +A++ D  G+E Y + + D  
Sbjct: 123 --------PEELVFDPNTLPGAEVFYSLGALEPSDDGRYIAFSFDLIGNERYELRVRDMT 182

Query: 227 TGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDM 286
            G  + +        L WA D+  ++ T +   R     + +L  E      ++ E ++ 
Sbjct: 183 NGREIWRD-PSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETGRSEVVFEEVNER 242

Query: 287 FSLDLEASESKKYLFI------ASESKFTRFN---FYLDVSRPEDGLVVLTPRVDGIDTY 346
            +L +  S S  YLFI         S+  R     + L   RP D    +  R  G + Y
Sbjct: 243 LALVVRRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWRRILARELGHEIY 302

Query: 347 PSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVIL 406
             H GN  F+ R ++   N  +V   +D+TS +    ++PHR  + ++EI +   H+++L
Sbjct: 303 AEHWGNE-FLFRVNDTGPNLRLVRTAIDDTSPSRWQEVVPHRAGITLEEIHVLEEHVIVL 362

Query: 407 EREDGLPKVVVYSLPD-IGEPLKTLEGGRAVDFA-DATYSVDALESEFSSSILRFCYSSM 466
           ERE   P++V +     +G  +  +E    V     A  S       +  S L +   S 
Sbjct: 363 EREGIQPRLVAHHRNGRVGPSIVPVEHSCTVTVGLSAGGSYSCARHPYRVSALTYKICSF 422

Query: 467 KTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVK 526
            TP     +D+ T  S +    T++ GF+   Y      A A DG +VP+SIV R+D   
Sbjct: 423 VTPDIFIQHDLLTDKSKV-LYRTLVSGFEPELYEARVVMAKAEDGVEVPISIVARRD--- 482

Query: 527 LDGSD-PLLLYGYGSY------EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWY 586
             G D P+LL  YG Y       +   PS   +R+SLLDRG  + I H+RGGGE+GR W+
Sbjct: 483 -RGEDGPVLLNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGGELGRAWH 542

Query: 587 ENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAI 646
           E     +K+ T TD I++AE L+E+++ S++ + I GRSAGG  + A   +RPDLF+A +
Sbjct: 543 EAATRDQKRLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLRPDLFRAVL 602

Query: 647 AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKA-QNYPDVLIT 690
           A VP  D++ T LD T+P    E  E+GDP     Y Y++SY P  N+   + YP   I 
Sbjct: 603 AEVPLADIIDTELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYNLTPDRRYPPTYID 639

BLAST of Cucsat.G10036.T13 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.1e-73
Identity = 205/682 (30.06%), Postives = 327/682 (47.95%), Query Frame = 0

Query: 38  HFRSPVATMNQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYT 97
           HFRS V   ++S  PP+         L  DV +D Y WLRD  R NPDV +YL  EN Y 
Sbjct: 26  HFRS-VVMWDKSLRPPLPRAEPRIRVLHDDVTVDRYGWLRD--RENPDVRAYLEAENSYA 85

Query: 98  DSVMSGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGE 157
           +   +  ++++ ++ +EI GR   +  T P + G + Y++    G  +  + RR V  G 
Sbjct: 86  EQATAHLRRLKTELIAEIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPVWWRRPVTGGS 145

Query: 158 EEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEI 217
            E                  ++LD N       +Y +G FE S + + +A++ D  G E 
Sbjct: 146 AE------------------LVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIGAER 205

Query: 218 YTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLH---KLGTEQS 277
           Y + + D   G  V +   G    + WA D+  ++ T +   RPD+   H   +L   + 
Sbjct: 206 YELRVRDMSDGRDVWRD-AGSVGQVVWAADNHTLFFTRE---RPDRRQHHQIVRLNVGRG 265

Query: 278 MDTCLYHEKDDMFSLDLEASESKKYLFI-----ASESKFTRFN----FYLDVSRPEDGLV 337
               ++ E ++  ++ +  S+S  +LF+     +  S + +      + L    P     
Sbjct: 266 NSEVVFEEANERLAVLVRRSQSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGGQWR 325

Query: 338 VLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQ 397
            +  R  G   Y  H  +  F+ R  +      +V+ P+D+ S +    ++PHR  V I 
Sbjct: 326 RIVMRELGHQIYAEHWYDR-FLFRVDDAGPYWRLVSAPIDDPSPSRWEEVVPHRAGVTID 385

Query: 398 EIELFLNHIVILEREDGLPKVV---------VYSLPDIGEPLKTLEGGRAVDFADATYSV 457
           EI +   H+V+LERE   P+++            +PD  EP  T+  G +   A   YS 
Sbjct: 386 EIHVLEQHLVLLEREGLRPRLISRNRSGRVGAVIVPD--EPSCTIRVGLS---AGGCYS- 445

Query: 458 DALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWAT 517
            A    F SS L +  SS  TP +  ++D     SV+   E  + G+D+ +Y+     A 
Sbjct: 446 -AARHPFRSSKLTYSVSSFVTPDTFIEHDFANDRSVV-LCEARVPGYDATQYLATVVMAE 505

Query: 518 ALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKA------SRISLLDRGF 577
           A DG +VP+S+V R+D        P+LL  YG Y     PSF A      +R+SLLDR  
Sbjct: 506 AEDGVQVPISLVARRDRT---SPGPVLLSVYGCYGIPRLPSFLAWPSSMTARLSLLDREV 565

Query: 578 IYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGG 637
            + I H+RGGGE+GR W++     +K+ T TD IS+ E LIE  + +++ + I G+S GG
Sbjct: 566 AFGIVHVRGGGELGRPWHDAATRDQKRITHTDLISATEGLIERGFATRDGVVIEGKSGGG 625

Query: 638 LLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSY 690
             + A    RP+LF+A +A VP  D++ T LD T+P T  E  E+GDP+    Y Y++SY
Sbjct: 626 GTVLATAVFRPNLFRAVVAEVPLADIIDTQLDSTMPYTLKETAEYGDPQDAYEYRYLRSY 670

BLAST of Cucsat.G10036.T13 vs. NCBI nr
Match: XP_004147101.1 (uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical protein Csa_008158 [Cucumis sativus])

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPDVLITAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689

BLAST of Cucsat.G10036.T13 vs. NCBI nr
Match: XP_008445904.1 (PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var. makuwa] >TYK15829.1 protease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036.T13 vs. NCBI nr
Match: XP_038893092.1 (protease 2 [Benincasa hispida])

HSP 1 Score: 1308 bits (3386), Expect = 0.0
Identity = 632/689 (91.73%), Postives = 666/689 (96.66%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQN IFG++RRS +L IPV+YLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MKPLQQNSIFGLVRRSLILLIPVVYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDFVMSGTKQVEEQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKG YYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGP+APPEHVIL
Sbjct: 121 EDDISVPERKGPYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPDAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPL GVTS
Sbjct: 181 DENVKAQNQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLAGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAG+DALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVS+PEDGLVVLTPRVDG+DT+PSHRGNHFFI RRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSKPEDGLVVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQ+I+LFLN I+I EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQDIQLFLNQIIIFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSVDA ESEFSSS+LRFCYSSMKTPPSTYDYDMKTGVS+LKKVETVLGG
Sbjct: 421 GGRAVDFTDATYSVDASESEFSSSVLRFCYSSMKTPPSTYDYDMKTGVSILKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD+NKYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFKASR
Sbjct: 481 FDTNKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEVCVDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFIS AEYLIENKY SKEKLC
Sbjct: 541 ISLLDRGFIYAIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYGSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIG+V+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGSVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036.T13 vs. NCBI nr
Match: XP_023541732.1 (uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1270 bits (3286), Expect = 0.0
Identity = 616/689 (89.40%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSMKTPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMKTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTF DFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFMDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 688

BLAST of Cucsat.G10036.T13 vs. NCBI nr
Match: KAG6573758.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1266 bits (3277), Expect = 0.0
Identity = 613/689 (88.97%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPV  KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSTAATMSNSHSPPVVKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YT+ VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

BLAST of Cucsat.G10036.T13 vs. ExPASy TrEMBL
Match: A0A0A0KV30 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1)

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPDVLITAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689

BLAST of Cucsat.G10036.T13 vs. ExPASy TrEMBL
Match: A0A5A7STX7 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003340 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036.T13 vs. ExPASy TrEMBL
Match: A0A1S3BDS7 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036.T13 vs. ExPASy TrEMBL
Match: A0A6J1G021 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1)

HSP 1 Score: 1265 bits (3273), Expect = 0.0
Identity = 613/689 (88.97%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YT+ VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKL TEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLETEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

BLAST of Cucsat.G10036.T13 vs. ExPASy TrEMBL
Match: A0A6J1HU74 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1)

HSP 1 Score: 1261 bits (3264), Expect = 0.0
Identity = 611/689 (88.68%), Postives = 651/689 (94.48%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  P  AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPLVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHF I RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFLIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSS+ILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSTILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD   YVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITNYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O078344.2e-13039.85Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
Q595361.1e-12738.38Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
P245552.5e-11939.06Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
P556568.8e-8031.17Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
P556276.1e-7330.06Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
XP_004147101.10.0100.00uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical ... [more]
XP_008445904.10.095.94PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var.... [more]
XP_038893092.10.091.73protease 2 [Benincasa hispida][more]
XP_023541732.10.089.40uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo][more]
KAG6573758.10.088.97Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KV300.0100.00Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1[more]
A0A5A7STX70.095.94Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
A0A1S3BDS70.095.94Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1[more]
A0A6J1G0210.088.97Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1[more]
A0A6J1HU740.088.68Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 545..569
score: 56.63
coord: 661..676
score: 51.97
coord: 519..537
score: 47.09
coord: 573..592
score: 47.89
coord: 603..623
score: 63.41
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 52..473
e-value: 2.3E-104
score: 349.5
NoneNo IPR availableGENE3D2.130.10.120coord: 122..476
e-value: 2.9E-222
score: 741.8
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 49..689
NoneNo IPR availablePANTHERPTHR11757:SF17B, PUTATIVE-RELATEDcoord: 49..689
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 48..482
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 534..688
e-value: 5.9E-52
score: 176.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 71..689
e-value: 2.9E-222
score: 741.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 486..688

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G10036Cucsat.G10036gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G10036.T13.E1Cucsat.G10036.T13.E1exon
Cucsat.G10036.T13.E2Cucsat.G10036.T13.E2exon
Cucsat.G10036.T13.E3Cucsat.G10036.T13.E3exon
Cucsat.G10036.T13.E4Cucsat.G10036.T13.E4exon
Cucsat.G10036.T13.E5Cucsat.G10036.T13.E5exon
Cucsat.G10036.T13.E6Cucsat.G10036.T13.E6exon
Cucsat.G10036.T13.E7Cucsat.G10036.T13.E7exon
Cucsat.G10036.T13.E8Cucsat.G10036.T13.E8exon
Cucsat.G10036.T13.E9Cucsat.G10036.T13.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G10036.T13.C1Cucsat.G10036.T13.C1CDS
Cucsat.G10036.T13.C2Cucsat.G10036.T13.C2CDS
Cucsat.G10036.T13.C3Cucsat.G10036.T13.C3CDS
Cucsat.G10036.T13.C4Cucsat.G10036.T13.C4CDS
Cucsat.G10036.T13.C5Cucsat.G10036.T13.C5CDS
Cucsat.G10036.T13.C6Cucsat.G10036.T13.C6CDS
Cucsat.G10036.T13.C7Cucsat.G10036.T13.C7CDS
Cucsat.G10036.T13.C8Cucsat.G10036.T13.C8CDS
Cucsat.G10036.T13.C9Cucsat.G10036.T13.C9CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G10036.T13Cucsat.G10036.T13-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity
molecular_function GO:0004722 protein serine/threonine phosphatase activity