Cucsat.G10036 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10036
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProlyl endopeptidase
Locationctg1673: 3679742 .. 3684908 (+)
RNA-Seq ExpressionCucsat.G10036
SyntenyCucsat.G10036
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTATAAAAAATATTTTCTACAATTATAGTTTCAAATTATATAAATGGAGAAATATTGAAAGCATAAAAAAGAGAAAAATTGTGATATTTAGAACAGTTTGAGAAGAAACCGTTGACTGAAATGACGCCTTTGCAACAAAACGGCATATTTGGAATACTTAGAAGAAGCTTCGTTCTCTTTATTCCCGTACTCTACCTTTCGCCGGTACTTCCGTCGGCAGCTTCATTCCGTCATTTCCGATCACCGGTTGCCACAATGAATCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGCATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCGAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATCGCTATACCGATTCCGTCATGTCCGGTGAACTCTAGTTTACATTTTCATTTCGGTTCTTGAGTTTTTTTTTTTGGTTACTACGTGAGAAATTTATGAATCAGTGATATGGTAGCGTTGATTTGATTTGAATGAGAGTAACGTCGTTTGATTTATCGATTGCTGATGATTTGTGTGTAATCTGGATTTTTTCTTTATTAAACGATATAGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTATTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATACTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCTAATGCTCCTCCGGAGCATGTTATACTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTATTGTAAAGACGGAACTGCTTCAGAATTTGATCACGTTTTCTCTTGAACAAAATTTTGATTCAAGGATCTTTATCGTCGTTTCTTCTCCAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGGGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGGGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGTGATTTACTGATTTTCTACCCTACATTCTGTACTTCTCATGGCTTTGACTGTTGTTTTCTCATTGGAAAAACAATTACTATCATGGGGTCTATAATCAAATAGGTCTCTTCATTTGTGGAATTAAAATATGAAGAAGACCAAATAAGTAAAAATCAATTTTAATCGAGGAGGAAATAATATTGCAAGGTCTTGAAGAGGACCTCCTAGACCATCCCGTCGATATCTGCATATGTCATCAGTTATTGTTGATTGTTCTGTGCATTCAGAATCGCCTTCTAATGATATTGTAGGTTGTTTCAGTAAAATGAAAATTCCTTACATACCATTAAATAGCTTGCATTCCTCTAGTTTTTCATTTGTAGTACTGATATATAGATTATAGTATCCTTATAATTTGATATCAGAAGAGTGGAGGCAGAGGAGTTTTTAGAGTTGTAAAGAGCGCTGCTCGAAGTACAAATTTTTGGTGAAAATACATTCAGTATCTGCATTCTGATGTTCCCAGTAATATTATTGTCGAACTTCAAATTGATAATTTCTTTTCAACGTTTTCAGAATTTTAGATGTTGATCTAGTAAATATTTTCCTCTTTCAATAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAGTATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCTTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAGGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGGTAGTGTTTCGAATTCTTTTATGATAATTGATGATTGACCTGATGGTATTATGTGAAGTTGAAACCCAATTGAAAGTGATGAGCTGATAGTTAATGACTTGAATCAACGAGCTTTAAAAGAAATCTTTGTCGGAACTTGGTGTTTATTTATTTAATTTGTTTGAAATATGTTAGGGTAGCTATTTTTATAAAATGAAAGACCCAACTGATCTATATCTAGGAGTGTAAGAAAAGAAAAGACAAGCTGAACTATAGCAAGGATTTTCATGTTCATTCGCTCTGACGTTTGGTACGTTGTTGGATTTATTCAACAAATTTAAATCTTATTGTGCTGTGAATCTGATTTTTTCTATACGAAATGTTAGAAGTTTCTTGATTAGCTAGTTATTGGGGCTTATGAAGTTGTTTGAATACTGTTCAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTACCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCGACTTATTCAGTGGATGCTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTAAGTAGTTCCACAATCATGGTTATGTTGTTCTCTTATGACCGGGATTGCCTCTAGGTTTAAATGAATTATTGTTAACCTTTAAATGTGATATTTTCAAATTTCCTGGTCATATTAGAAAAATATATATAAACCAGATATGAATTTCTATTCTCTGTTTTCTCAACCTAGTACTACCCATTTGTGGCTAGGAGTATATTTCATTTATGTATCTAGTCTTTCTAAAACCAGTGCTAACTTCAACTTTGAGTACATCCTTTTGCTTCCTTTGTTTCTTTTTCTTTCACTTCTTACGATTAAGAATTTTTATTCCCTATATGCTAGTGATGGTTTAGAAAAATCTTCCGCCTGTCTCTTAGTTGATTTTATGCCTTTTCATCGTCCGTTTTTTGTTGTTGTTGTTGAGAATTCTTGTTGTCTAGCGAATATAAAAAGTCTCCTCTTTTCATCGTCCGTTTTTTGTTGTTGTTGTTGTTGAGAATTCTTGTTGTCTAGCGAATATAAAAAGTCTCCTCCTTACCTTTCCTTCCCCCCAGGAGGATTTGAATTAATTCATCTTACTGTAACGTAGTGAAATTTCCACAAATTTTCTCAGGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGGTAACAAGTCTTGTGTTAATTAGACTCTATACGGATCTCAGGAACTCGAAGTTATTTTAAATTGAAAGAGTGTTTTTGTTTCCTGGGGACTACTCCCCCTATGAGTCAGAGTTTCTTTAATGGAGCTTCAAATAACGTGATTATAAATTTATAAGTTATTTTCCTGTTTTAATTGATTTATCAAAATTTCCAATTCTGAAATATTTATTTCTGCATTGTAATGGATTCTTTGAGGATCTATGTTTCGTTTTGATGTCCAGTGTTTTTGCTATAATTACGATCTCTGCGTTGCTAATTTACAGTTCTTAAACGTTTGAGGACATGAACTTGTACCTGAATCTTTTTGTTTACTTTTTTCTTGTCACTTAATTGAGTTTTGAATTGTAACAATGATGGTACATGAACCTGATCTCGAAGTGAATTTCATTCTTTGAACAGTATTGCATAGACCCTAGTTTCAAGGCATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACGGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGACGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGTAAATCTTCCCTAGCTAGTGTACCTTTCGCAATTCTGTAGGTGACATGCAGTGAATTAGTGCAATTCCTTGGTCAGTACTTAAATAGACAAGTTAAGGACTCTTTAACTTTAGCGGCATTGGCTCAAAATCACTTTTGAGTCCCCAAATTATGGTGCCATTTTGAACTAGAGTCCAATGTAGGATAGGCAGTTATCAGTTTAACAATATTATGTATTGAAAAAAACAAAAGGGTGGAAAGTGTCCCTGTTTGTAGCACGGTTCCTAACATACGAGATTTTCTTTTGACTCTGTAAATATCTTTACTTATCCATTATCTTCTTTTTTCACTTTTTTCAACTGTCGTTTACTGAAAATATGTTGTATAACTAATTGCAGCGCCTATTCTTTTTGACTTGCCACTTACCTTCTTTTGAATTCTGCAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGACAATGTACGCACCTCACTATTTTTTAATTAACATATTCTGTTTCAGCCATTTCCATCTAATCAATCATCCCCCTTGATCGCAACTTGATTTTGGATCATTTGACTGTGTCCGTGCTTATGTGTTTCATAATTCATAGAATATTTTCAAACTTTTGTTTTGATTAAGACATGACTTTATCTTAATGTTTCAGGTTAAGGCTCAAAATTATCCGGACGTACTTATTACTGCCGGCTTAAACGGTAAGTGTGATGGCTTAAAACTTCAATTCGAGTAGAGCCCCAATCAATTTTCCTGTTTCTTTATCCCGTGCACACATTCATTCTTCATATCCAGAATAGACAAGGTCACAAGGAGATTGATGAATATATGGATGTCTGGGAGTGATTTTGGTCACGTTGAAAATCATTCTAAAGCATGCTTTTACACTTAAAATTAGATTAATATTTCATTTTATGCTTTTAAATGTAATTTTCATATCATAAAAAATAATTAATTTTATTTGAATGATGAAAAAGCATCTTTTACAATGATTTTAAAAATGTTAAAAGTGGTTTTAATCCTTTCAAAATCACACTCCAGGTGTGCCCAATCAAACATTCCTTCAAAATGTTAAATCCTTTTTTTGGTCCATTGGTCTATGTGGCAGATCCACGTGTCTTATATTCAGAACCGGCTAAGTTTGTGGCAAAATTAAGGGCTACGAAGACTGATAATAATCTTCTGCTTTTCAAATGTGAACTTGGTGCTGGACATTTTTCAAAATCAGGAAGGTAATTATTCGATTGTTGATTTCTTTTGTGGGCTTCGTGATCTGTGTTTTCTTTTCTTGTTTGTATCTCCTCTCTTTTTTGTTGCATGTGTGCCTTTTCTTCTTCCTCAACATGATTATAAAATTATTTCTTATTTAATTGATGATATTCACTGCTGCCTTTTTTATTTGTAACTCTACGGTACACATGGAGAAGAAACATTGTTTTGATCTCTGTTATGATTTCTCACTTCATATTCATATACCTGTAACTTTTTTGGTAGAACTTTATTATTTTTCATACTAGTGTAGGACTTTTTTATGAAGTATGAAGAAAAGAAAGAAAAAGCTATTCTAAAACAGACCTTTAGTTAGGTCTTATTCTCTTGGATTTAAGTCATTTTCTTAATTTATGTAGGACTCCTTTTTGTAGAGTGGTTTTTTTTAATACTTTGTATGTTCATTCATTTCTCTGAGTTTCTATTATTTTTTTTTTGCAAAAGTTGGCCACTCGTGATAATAAACTTAGGAAAAGATAAAGGAAAACAACCTTGAGAATACAAAAAGAACATCTTTACATTAATCTTTTGTTATTAAAAAAATAAAGAGCTCATCCTTCGTTGTTAGGGGTCGATGTTCTGGAGCATTTAAAAGATACTTACATTTTCTGGTTTTTGATTTATTATTATTATTATTCTTTTGCTGCCATTTCAGATTTGAGAAGCTTCAAGAGGATGCATTCACATACGCTTTTATACTGAAGTCTCTTAACATGATTCCAGCAGTCGGAAATTGAGCAGAAAAAGCTGCTCCCTTGTTGGTACACTTTTTTCATTCTTCTATTGACCTTTTTAGTGAAAAATCTTTCTTTACTACAAAAAGGGTCATGTTTGTACTGTTCTTCATTACCTTACCATTAGATAACCTATGCTATTTTAAGATAACTAGTTAGCATTCATGTGCTTTTGCACCAACATTTTTAGAAATTCAAACTAGTTTACTATTCTCTCAATAACATTTGAGAATGTAACCAAAGTGAGATATTGGTTAGGAAATTGAAAGTCCAACTATATATCGGTATTGGTATTAGGTCTCTTGGGTAAAACTTAAAACCAAAGACTATGGGGGAACATTTTCTATACTACGGGAGTAGGAAAAAATTTCGTACCACTTCTAGTTATTTTGTGTCACATTAACACACTTTAAAAATAAAACAGAGCCCACTGCATTAGCGTGTGGTACACAAATATTTATGAAGACAAATTTATAGTGTTCACAAGGAAGTAGTACTCTCGCAGTACTGAGAAGACCACGTTTGTTTTATGGGAAGTCATGCTTGGGTTTTGACTCGGATAAAAGTTTCTATGTTTGTTTTATGGATTTTGAAACTTGAGAACTTAAAAATGTTTTTTGAACCAAGAGATTCTTCACTTGAACCCTTTGATTTTTTTTTTCAAGGGATATTTTGTTTGAAGAGAGTTAAAAGACGTGATTTGGATGTGTTTAATACGTTTTTATTATTTTATGAAAAATATTTAGTTGGATCTAACTTTGTAAAAACTCTT

Coding sequence (CDS)

ATGACGCCTTTGCAACAAAACGGCATATTTGGAATACTTAGAAGAAGCTTCGTTCTCTTTATTCCCGTACTCTACCTTTCGCCGGTACTTCCGTCGGCAGCTTCATTCCGTCATTTCCGATCACCGGTTGCCACAATGAATCAATCTCACTCTCCTCCGGTCGCCAACAAGGTGGAGCATAAAATGGAGTTGTTCGGAGACGTAAGGATCGACAACTATTACTGGTTACGTGACGATTCTCGCTCGAATCCTGATGTCCTTTCGTATCTGCGGGAGGAGAATCGCTATACCGATTCCGTCATGTCCGGAACAAAGAAAGTGGAAGACCAGATTTTTAGTGAGATAAGAGGACGAATCAAGGAGGATGATATAACTGTGCCTGAACGGAAAGGTTCTTACTATTACTATGAGAGGACTTTGGAAGGGAAAGAATATGTTCAATACTGTAGGCGTTTTGTTCCTCGTGGTGAAGAAGAAGCTATATCTGTGCACGATACCATGCCCACTGGACCTAATGCTCCTCCGGAGCATGTTATACTGGATGAAAATGTTAAGGCCGAGAATCAATCTTACTACTCCATTGGTTGCTTTGAGGTTAGTCCAAACAACAAGCTAGTGGCGTATGCAGAAGACACTAAAGGAGATGAAATCTACACGGTTTATATAATCGATGCCGAGACTGGGGCTCCTGTGGGAAAGCCTCTTGTAGGTGTGACCTCGTATCTCAAATGGGCTGGGGATGATGCTTTAGTTTACATCACAATGGATGAGATTCTTCGACCTGATAAGGCATGGTTACATAAACTGGGAACAGAGCAGTCAATGGACACCTGCCTTTATCATGAAAAGGATGACATGTTCTCTCTTGATCTTGAAGCTTCTGAGAGCAAGAAGTATTTGTTTATTGCATCTGAAAGTAAATTTACTCGGTTCAACTTTTATCTTGATGTATCAAGGCCCGAAGATGGGCTTGTTGTCTTGACACCTAGAGTGGATGGAATTGACACTTATCCCAGTCATCGTGGAAATCATTTTTTTATCCTGAGACGAAGTGAGGAGATTTTTAATTCAGAAGTAGTAGCTTGCCCTCTTGATAATACATCAGCTACCACAGTTATTCTTCCGCACAGGGAAAGTGTGAAAATCCAGGAAATCGAACTATTTCTTAACCACATCGTCATATTGGAACGTGAAGATGGTCTACCAAAAGTTGTTGTCTATAGCCTTCCAGATATTGGAGAACCACTTAAAACCCTTGAAGGAGGACGAGCTGTGGATTTTGCTGATGCGACTTATTCAGTGGATGCTTTAGAATCAGAATTCTCTTCTAGTATTTTACGGTTTTGTTACAGCTCAATGAAGACACCCCCCTCTACATATGATTATGATATGAAAACAGGAGTTTCCGTTCTAAAGAAAGTTGAAACAGTGTTGGGAGGTTTTGATTCTAATAAATATGTCACAGAGAGGAAATGGGCAACTGCTTTAGATGGCACTAAAGTTCCCCTATCAATTGTTTACCGAAAGGATCTAGTGAAACTGGATGGTTCAGACCCACTTCTACTTTACGGCTATGGTTCTTATGAGTATTGCATAGACCCTAGTTTCAAGGCATCAAGGATATCTTTGTTAGACAGAGGTTTTATTTATGTAATAGCTCACATTCGCGGGGGTGGTGAAATGGGGAGGCAGTGGTATGAAAATGGAAAGTTACTGAAGAAAAAAAATACATTCACGGATTTTATTTCTTCTGCTGAATACTTGATCGAGAATAAATACTGTTCAAAGGAAAAACTATGCATCAATGGAAGAAGTGCTGGTGGTTTGCTTATTGGTGCTGTTATAAATATGAGGCCAGATTTGTTCAAAGCTGCAATTGCTGGGGTACCTTTTGTAGACGTTCTAACGACCATGCTTGATCCCACAATTCCTCTTACAACTTCGGAGTGGGAGGAATGGGGTGACCCTAGGAAGGAGGAATTCTACTTTTACATGAAATCATATTCTCCGGTTGACAATGTTAAGGCTCAAAATTATCCGGACGTACTTATTACTGCCGGCTTAAACGATCCACGTGTCTTATATTCAGAACCGGCTAAGTTTGTGGCAAAATTAAGGGCTACGAAGACTGATAATAATCTTCTGCTTTTCAAATGTGAACTTGGTGCTGGACATTTTTCAAAATCAGGAAGATTTGAGAAGCTTCAAGAGGATGCATTCACATACGCTTTTATACTGAAGTCTCTTAACATGATTCCAGCAGTCGGAAATTGA

Protein sequence

MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLEGGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLNDPRVLYSEPAKFVAKLRATKTDNNLLLFKCELGAGHFSKSGRFEKLQEDAFTYAFILKSLNMIPAVGN
Homology
BLAST of Cucsat.G10036 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 4.2e-130
Identity = 259/650 (39.85%), Postives = 382/650 (58.77%), Query Frame = 0

Query: 49  SHSPPVANKVEHKMEL-FGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKV 108
           S +PP   K  H ++   G  R D YYWLRDD R N ++L+YL  EN YTD+VM+  K +
Sbjct: 25  SATPPDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYTDAVMAPLKPL 84

Query: 109 EDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTM 168
           ED+++ E+  RIK+DD +VP R+  ++YY R + GK+Y  + RR    G  +A+S+    
Sbjct: 85  EDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPG-VDAVSIQAAN 144

Query: 169 PTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAET 228
             G  A  E V+LD N     + YY++G +EVS +N+L+AYA+DT G   YT+   + +T
Sbjct: 145 AAGDFA-GEQVLLDVNALGAGKDYYNVGDYEVSQDNRLLAYADDTNGRRQYTIRFKNLDT 204

Query: 229 GAPVGKPLVGVTSYLKWAGDD-ALVYITMD-EILRPDKAWLHKLGTEQSMDTCLYHEKDD 288
           G  +   +      L W+ D   L Y+  D E L   +   H LGT  S D  +Y E+DD
Sbjct: 205 GELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQDALVYEEEDD 264

Query: 289 MFSLDLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHF 348
            F + +  S   K++ I+ ES  +        + P     VL PR   ++    H G+ +
Sbjct: 265 SFYMGIGRSRDDKFICISVESTVSSEMRCTPAASP-GVFTVLAPRERDVEYQADHLGDRW 324

Query: 349 FILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVILEREDGLPK 408
            I   ++   N ++V  P D+TS       + HR+ V ++  ELF    V+ ER + L  
Sbjct: 325 VIRTNADGATNFKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGFSVVAERANAL-- 384

Query: 409 VVVYSLPDIGEPLKTLEGGRAVDFADATYSVDAL----ESEFSSSILRFCYSSMKTPPST 468
                     E L+ ++   + D+  A  S  ++      E  +  LR+ Y+SM TP +T
Sbjct: 385 ----------ESLRVIKADGSSDYVKADESAYSMGLSANPETGTDWLRYSYTSMTTPATT 444

Query: 469 YDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDG-TKVPLSIVYRKDLVKLDGSD 528
           Y+ + KTG     K + V  G+D++KYVTER WA A DG TK+P+++VYRKD+ + DG  
Sbjct: 445 YEINTKTGERRQLKQQPV-PGYDASKYVTERVWAPARDGKTKIPVTLVYRKDVAR-DGKA 504

Query: 529 PLLLYGYGSYEYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTF 588
           P+L Y YGSY   +DP+F  + +SLLDRG +Y +AHIRGG EMGR WY++GKL  K NTF
Sbjct: 505 PMLQYAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMGRAWYDDGKLYNKINTF 564

Query: 589 TDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTM 648
           TDFI   +YL++  Y +K+++   G SAGGLL+GAV NM P+ +K  +  VPFVDV+TTM
Sbjct: 565 TDFIDVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVDVVTTM 624

Query: 649 LDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGL 689
           LDPTIPLTT+E++EWG+P ++ +Y Y+ +YSP DN++A+ YP + +  GL
Sbjct: 625 LDPTIPLTTNEYDEWGNPEEKGYYDYILTYSPYDNLQAKAYPAMFVGTGL 657

BLAST of Cucsat.G10036 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.1e-127
Identity = 246/641 (38.38%), Postives = 377/641 (58.81%), Query Frame = 0

Query: 53  PVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIF 112
           P+A ++ H  EL GDVR D+YYWL+D  R N +V+ YL EENRY   +M   ++  +QI+
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKD--RDNTEVIQYLEEENRYYHEIMRPLQEQTEQIY 63

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
             +  R+ + ++ VP + G ++YY R  + K+Y  Y R+   +    A+    T      
Sbjct: 64  ESMVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARK---QAASRALLQDAT------ 123

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
              E V+LD N  AE   Y S+    ++ ++  +AY E+  G + YT+YI D  TG  + 
Sbjct: 124 ---EEVVLDLNELAEEDDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNTGELLS 183

Query: 233 K--PLVGVTSYLKWAG-DDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSL 292
              P V +   ++W    D + Y T+DE  RP + W H+LG++   D  ++ EKDD F+L
Sbjct: 184 DRVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKDDTFTL 243

Query: 293 DLEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILR 352
            +  S+S K++F+ S SK T     +D   P   L ++  R DGI     H  +   IL 
Sbjct: 244 FISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDDLLIL- 303

Query: 353 RSEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSL 412
            +E   N +++ CPL++ S+   ++ + E   +QE+  F + ++I  RE+GL ++ V   
Sbjct: 304 TNEGALNFQLLRCPLNDLSSKVNVVEYNEERYLQEMYPFRDKLLIAGRENGLTQIWVVHD 363

Query: 413 PDIGEPLKTLEGGRAVDFADATYSVDAL-ESEFSSSILRFCYSSMKTPPSTYDYDMKTGV 472
            ++          + + + +  Y+V  L E  + ++ +   Y S+ TP +T+  +++TG 
Sbjct: 364 GEL----------QQISWDEPLYTVAVLSEQSYDTNEVLIQYESLLTPKTTFGLNLQTGE 423

Query: 473 SVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSY 532
               +V  V G +D +++  E+ WAT   G KVP++ VY +  +  +G  PL+LYGYGSY
Sbjct: 424 KQCLQVAPVSGEYDRSQFRQEQLWATGRSGVKVPMTAVYLEGALD-NGPAPLILYGYGSY 483

Query: 533 EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYL 592
               DP F   R+ LL++G ++V A +RGG EMGR WYE+GK+  K+NTFTDFI++A++L
Sbjct: 484 GSNSDPRFDPYRLPLLEKGIVFVTAQVRGGSEMGRGWYEDGKMQNKRNTFTDFIAAAKHL 543

Query: 593 IENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTS 652
           I+  Y S  K+   G SAGGLL+GAV NM  +LFK  +  VPFVDV+TTMLD +IPLTT 
Sbjct: 544 IDQNYTSPTKMAARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTL 603

Query: 653 EWEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLN 690
           EW+EWGDPRK+E YFYMKSYSP DNV+A++YP + IT G+N
Sbjct: 604 EWDEWGDPRKQEDYFYMKSYSPYDNVEAKDYPHMYITTGIN 618

BLAST of Cucsat.G10036 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 431.0 bits (1107), Expect = 2.5e-119
Identity = 250/640 (39.06%), Postives = 351/640 (54.84%), Query Frame = 0

Query: 53  PVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIF 112
           P A ++ H M L GD RIDNYYWLRDD+RS P+VL YL++EN Y   VM+  + ++D+I 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 113 SEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPN 172
            EI  RI + +++ P  K  Y Y      G EY  Y R+     E +             
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWD------------- 122

Query: 173 APPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVG 232
                 +LD N +A +  +YS+G   ++P+N ++A AED      Y +   + ETG    
Sbjct: 123 --EWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNLETGNWYP 182

Query: 233 KPLVGVTSYLKWAGDDALVYITMDE--ILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLD 292
           + L  V     WA D  + Y        L P + W H +GT  S D  +Y EKDD + + 
Sbjct: 183 ELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVS 242

Query: 293 LEASESKKYLFIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRR 352
           L  + SK Y+ I   S  T     LD    +    V  PR    + Y      H F LR 
Sbjct: 243 LHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHE-YSLDHYQHRFYLRS 302

Query: 353 SEEIFNSEVVACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLP 412
           +    N  +    + +      ++P RE++ ++   LF + +V+ ER+ GL      SL 
Sbjct: 303 NRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLT-----SLR 362

Query: 413 DIGEPLKTLEGGRAVDFADATY-SVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVS 472
            I    + + G   + F D  Y +  A   E  ++ LR+ YSSM TP + ++ DM TG  
Sbjct: 363 QINRKTREVIG---IAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGER 422

Query: 473 VLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE 532
            + K +T + GF +  Y +E  W  A DG +VP+S+VY +   +  G +PLL+YGYGSY 
Sbjct: 423 RVLK-QTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFR-KGHNPLLVYGYGSYG 482

Query: 533 YCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLI 592
             ID  F  SR+SLLDRGF+Y I H+RGGGE+G+QWYE+GK LKKKNTF D++ + + L+
Sbjct: 483 ASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALL 542

Query: 593 ENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSE 652
           +  Y S       G SAGG+L+G  IN RP+LF   IA VPFVDV+TTMLD +IPLTT E
Sbjct: 543 KLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGE 602

Query: 653 WEEWGDPRKEEFYFYMKSYSPVDNVKAQNYPDVLITAGLN 690
           +EEWG+P+  ++Y YMKSYSP DNV AQ YP +L+T GL+
Sbjct: 603 FEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLH 616

BLAST of Cucsat.G10036 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.8e-80
Identity = 207/664 (31.17%), Postives = 322/664 (48.49%), Query Frame = 0

Query: 47  NQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKK 106
           N+S  PP+         L  DV ID+Y WLRD  R +PDVL+YL  EN Y D V S   +
Sbjct: 3   NKSLQPPLPRSERRIRVLHNDVTIDSYGWLRD--REDPDVLAYLEAENHYADEVTSYVAE 62

Query: 107 VEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDT 166
           ++  + +EI  R   D    P + G ++Y++++  G  +  + RR V  G          
Sbjct: 63  LKADLIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSGLLHSAWWRRPVTGG---------- 122

Query: 167 MPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAE 226
                   PE ++ D N     + +YS+G  E S + + +A++ D  G+E Y + + D  
Sbjct: 123 --------PEELVFDPNTLPGAEVFYSLGALEPSDDGRYIAFSFDLIGNERYELRVRDMT 182

Query: 227 TGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDM 286
            G  + +        L WA D+  ++ T +   R     + +L  E      ++ E ++ 
Sbjct: 183 NGREIWRD-PSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETGRSEVVFEEVNER 242

Query: 287 FSLDLEASESKKYLFI------ASESKFTRFN---FYLDVSRPEDGLVVLTPRVDGIDTY 346
            +L +  S S  YLFI         S+  R     + L   RP D    +  R  G + Y
Sbjct: 243 LALVVRRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWRRILARELGHEIY 302

Query: 347 PSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQEIELFLNHIVIL 406
             H GN  F+ R ++   N  +V   +D+TS +    ++PHR  + ++EI +   H+++L
Sbjct: 303 AEHWGNE-FLFRVNDTGPNLRLVRTAIDDTSPSRWQEVVPHRAGITLEEIHVLEEHVIVL 362

Query: 407 EREDGLPKVVVYSLPD-IGEPLKTLEGGRAVDFA-DATYSVDALESEFSSSILRFCYSSM 466
           ERE   P++V +     +G  +  +E    V     A  S       +  S L +   S 
Sbjct: 363 EREGIQPRLVAHHRNGRVGPSIVPVEHSCTVTVGLSAGGSYSCARHPYRVSALTYKICSF 422

Query: 467 KTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWATALDGTKVPLSIVYRKDLVK 526
            TP     +D+ T  S +    T++ GF+   Y      A A DG +VP+SIV R+D   
Sbjct: 423 VTPDIFIQHDLLTDKSKV-LYRTLVSGFEPELYEARVVMAKAEDGVEVPISIVARRD--- 482

Query: 527 LDGSD-PLLLYGYGSY------EYCIDPSFKASRISLLDRGFIYVIAHIRGGGEMGRQWY 586
             G D P+LL  YG Y       +   PS   +R+SLLDRG  + I H+RGGGE+GR W+
Sbjct: 483 -RGEDGPVLLNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGGELGRAWH 542

Query: 587 ENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGGLLIGAVINMRPDLFKAAI 646
           E     +K+ T TD I++AE L+E+++ S++ + I GRSAGG  + A   +RPDLF+A +
Sbjct: 543 EAATRDQKRLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLRPDLFRAVL 602

Query: 647 AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVKA-QNYPDVLIT 690
           A VP  D++ T LD T+P    E  E+GDP     Y Y++SY P  N+   + YP   I 
Sbjct: 603 AEVPLADIIDTELDFTLPYALRETAEYGDPHLANDYQYLRSYDPYYNLTPDRRYPPTYID 639

BLAST of Cucsat.G10036 vs. ExPASy Swiss-Prot
Match: P55627 (Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01920 PE=3 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.1e-73
Identity = 205/682 (30.06%), Postives = 327/682 (47.95%), Query Frame = 0

Query: 38  HFRSPVATMNQSHSPPVANKVEHKMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYT 97
           HFRS V   ++S  PP+         L  DV +D Y WLRD  R NPDV +YL  EN Y 
Sbjct: 26  HFRS-VVMWDKSLRPPLPRAEPRIRVLHDDVTVDRYGWLRD--RENPDVRAYLEAENSYA 85

Query: 98  DSVMSGTKKVEDQIFSEIRGRIKEDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGE 157
           +   +  ++++ ++ +EI GR   +  T P + G + Y++    G  +  + RR V  G 
Sbjct: 86  EQATAHLRRLKTELIAEIEGRQPCEGATPPFQVGPFDYFQGHERGLPHPVWWRRPVTGGS 145

Query: 158 EEAISVHDTMPTGPNAPPEHVILDENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEI 217
            E                  ++LD N       +Y +G FE S + + +A++ D  G E 
Sbjct: 146 AE------------------LVLDPNAIPGADVFYWLGVFEPSDDGRYLAFSVDLIGAER 205

Query: 218 YTVYIIDAETGAPVGKPLVGVTSYLKWAGDDALVYITMDEILRPDKAWLH---KLGTEQS 277
           Y + + D   G  V +   G    + WA D+  ++ T +   RPD+   H   +L   + 
Sbjct: 206 YELRVRDMSDGRDVWRD-AGSVGQVVWAADNHTLFFTRE---RPDRRQHHQIVRLNVGRG 265

Query: 278 MDTCLYHEKDDMFSLDLEASESKKYLFI-----ASESKFTRFN----FYLDVSRPEDGLV 337
               ++ E ++  ++ +  S+S  +LF+     +  S + +      + L    P     
Sbjct: 266 NSEVVFEEANERLAVLVRRSQSGAWLFLDVLTTSDMSSYVQRGAAEVWCLPADEPGGQWR 325

Query: 338 VLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVVACPLDNTSAT--TVILPHRESVKIQ 397
            +  R  G   Y  H  +  F+ R  +      +V+ P+D+ S +    ++PHR  V I 
Sbjct: 326 RIVMRELGHQIYAEHWYDR-FLFRVDDAGPYWRLVSAPIDDPSPSRWEEVVPHRAGVTID 385

Query: 398 EIELFLNHIVILEREDGLPKVV---------VYSLPDIGEPLKTLEGGRAVDFADATYSV 457
           EI +   H+V+LERE   P+++            +PD  EP  T+  G +   A   YS 
Sbjct: 386 EIHVLEQHLVLLEREGLRPRLISRNRSGRVGAVIVPD--EPSCTIRVGLS---AGGCYS- 445

Query: 458 DALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGGFDSNKYVTERKWAT 517
            A    F SS L +  SS  TP +  ++D     SV+   E  + G+D+ +Y+     A 
Sbjct: 446 -AARHPFRSSKLTYSVSSFVTPDTFIEHDFANDRSVV-LCEARVPGYDATQYLATVVMAE 505

Query: 518 ALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKA------SRISLLDRGF 577
           A DG +VP+S+V R+D        P+LL  YG Y     PSF A      +R+SLLDR  
Sbjct: 506 AEDGVQVPISLVARRDRT---SPGPVLLSVYGCYGIPRLPSFLAWPSSMTARLSLLDREV 565

Query: 578 IYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLCINGRSAGG 637
            + I H+RGGGE+GR W++     +K+ T TD IS+ E LIE  + +++ + I G+S GG
Sbjct: 566 AFGIVHVRGGGELGRPWHDAATRDQKRITHTDLISATEGLIERGFATRDGVVIEGKSGGG 625

Query: 638 LLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSY 690
             + A    RP+LF+A +A VP  D++ T LD T+P T  E  E+GDP+    Y Y++SY
Sbjct: 626 GTVLATAVFRPNLFRAVVAEVPLADIIDTQLDSTMPYTLKETAEYGDPQDAYEYRYLRSY 670

BLAST of Cucsat.G10036 vs. NCBI nr
Match: XP_004147101.1 (uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical protein Csa_008158 [Cucumis sativus])

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPDVLITAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689

BLAST of Cucsat.G10036 vs. NCBI nr
Match: XP_008445904.1 (PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var. makuwa] >TYK15829.1 protease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036 vs. NCBI nr
Match: XP_038893092.1 (protease 2 [Benincasa hispida])

HSP 1 Score: 1308 bits (3386), Expect = 0.0
Identity = 632/689 (91.73%), Postives = 666/689 (96.66%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQN IFG++RRS +L IPV+YLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MKPLQQNSIFGLVRRSLILLIPVVYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDFVMSGTKQVEEQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKG YYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGP+APPEHVIL
Sbjct: 121 EDDISVPERKGPYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPDAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPL GVTS
Sbjct: 181 DENVKAQNQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLAGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAG+DALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGNDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVS+PEDGLVVLTPRVDG+DT+PSHRGNHFFI RRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSKPEDGLVVLTPRVDGVDTFPSHRGNHFFIRRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQ+I+LFLN I+I EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQDIQLFLNQIIIFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSVDA ESEFSSS+LRFCYSSMKTPPSTYDYDMKTGVS+LKKVETVLGG
Sbjct: 421 GGRAVDFTDATYSVDASESEFSSSVLRFCYSSMKTPPSTYDYDMKTGVSILKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD+NKYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFKASR
Sbjct: 481 FDTNKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEVCVDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIY IAHIRGGGEMGRQWYENGKLLKKKNTFTDFIS AEYLIENKY SKEKLC
Sbjct: 541 ISLLDRGFIYAIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISCAEYLIENKYGSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIG+V+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGSVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036 vs. NCBI nr
Match: XP_023541732.1 (uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1270 bits (3286), Expect = 0.0
Identity = 616/689 (89.40%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSMKTPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMKTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTF DFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFMDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 688

BLAST of Cucsat.G10036 vs. NCBI nr
Match: KAG6573758.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1266 bits (3277), Expect = 0.0
Identity = 613/689 (88.97%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPV  KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSTAATMSNSHSPPVVKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YT+ VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

BLAST of Cucsat.G10036 vs. ExPASy TrEMBL
Match: A0A0A0KV30 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1)

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH
Sbjct: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPDVLITAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689

BLAST of Cucsat.G10036 vs. ExPASy TrEMBL
Match: A0A5A7STX7 (Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003340 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036 vs. ExPASy TrEMBL
Match: A0A1S3BDS7 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 661/689 (95.94%), Postives = 675/689 (97.97%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           MT LQQN IFGILRRSF+LFIPVLYLSP LPS ASFRHFRSPVATM+QSHSPPVANKVEH
Sbjct: 1   MTLLQQNSIFGILRRSFILFIPVLYLSPALPSVASFRHFRSPVATMSQSHSPPVANKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR NPDVLSYL++EN YTDSVMSGTKKVE QI+SEIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNPDVLSYLQQENAYTDSVMSGTKKVEQQIYSEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDD+TVPER+GSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL
Sbjct: 121 EDDVTVPERRGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS
Sbjct: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+ASESKKYL
Sbjct: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSKDTCLYHEKDDMFSLDLQASESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDN SATTVILPHRESVKIQ+I+LFLNHIVI EREDGLPKVVVYSLPDIGEPLKTLE
Sbjct: 361 ACPLDNISATTVILPHRESVKIQDIQLFLNHIVIFEREDGLPKVVVYSLPDIGEPLKTLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDFADATYSVDA ESEFSSSILRFCYSSMKTP STYDYDMKTGVSVLKKVETVLGG
Sbjct: 421 GGRAVDFADATYSVDAEESEFSSSILRFCYSSMKTPLSTYDYDMKTGVSVLKKVETVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEICVDPSFKPSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAVINMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVINMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQNYPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQNYPDILVTAGLN 689

BLAST of Cucsat.G10036 vs. ExPASy TrEMBL
Match: A0A6J1G021 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1)

HSP 1 Score: 1265 bits (3273), Expect = 0.0
Identity = 613/689 (88.97%), Postives = 653/689 (94.78%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  PS AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPSVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YT+ VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTELVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKL TEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLETEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHFFI RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFFIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPLK+LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLKSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSSSILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSSILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD  KYVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITKYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKL+KKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLMKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

BLAST of Cucsat.G10036 vs. ExPASy TrEMBL
Match: A0A6J1HU74 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1)

HSP 1 Score: 1261 bits (3264), Expect = 0.0
Identity = 611/689 (88.68%), Postives = 651/689 (94.48%), Query Frame = 0

Query: 1   MTPLQQNGIFGILRRSFVLFIPVLYLSPVLPSAASFRHFRSPVATMNQSHSPPVANKVEH 60
           M PLQQNGIFG++RRS VLF+PV+ LSP  P  AS RHFRS  ATM+ SHSPPVA KVEH
Sbjct: 1   MKPLQQNGIFGLVRRSLVLFLPVVLLSPPPPLVASLRHFRSAAATMSNSHSPPVAKKVEH 60

Query: 61  KMELFGDVRIDNYYWLRDDSRSNPDVLSYLREENRYTDSVMSGTKKVEDQIFSEIRGRIK 120
           KMELFGDVRIDNYYWLRDDSR N DV+SYL++EN YTD VMSGTK+VE+QI++EIRGRIK
Sbjct: 61  KMELFGDVRIDNYYWLRDDSRKNSDVISYLQQENAYTDLVMSGTKQVEEQIYAEIRGRIK 120

Query: 121 EDDITVPERKGSYYYYERTLEGKEYVQYCRRFVPRGEEEAISVHDTMPTGPNAPPEHVIL 180
           EDDI+VPERKGSYYYYERTL+GKEYVQYCRRFVPRG EE++SVHDTMPTGP APPEHVIL
Sbjct: 121 EDDISVPERKGSYYYYERTLQGKEYVQYCRRFVPRG-EESLSVHDTMPTGPGAPPEHVIL 180

Query: 181 DENVKAENQSYYSIGCFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGAPVGKPLVGVTS 240
           DENVKA+NQSYYSIG FEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGA VGKPLVGVTS
Sbjct: 181 DENVKAQNQSYYSIGAFEVSPNNKLVAYAEDTKGDEIYTVYIIDAETGASVGKPLVGVTS 240

Query: 241 YLKWAGDDALVYITMDEILRPDKAWLHKLGTEQSMDTCLYHEKDDMFSLDLEASESKKYL 300
           YL WAGDDALVYITMDEILRPDKAWLHKLGTEQS DTCLYHEKDDMFSLDL+A+ESKKYL
Sbjct: 241 YLNWAGDDALVYITMDEILRPDKAWLHKLGTEQSTDTCLYHEKDDMFSLDLQATESKKYL 300

Query: 301 FIASESKFTRFNFYLDVSRPEDGLVVLTPRVDGIDTYPSHRGNHFFILRRSEEIFNSEVV 360
           FIASESKFTRFNFYLDVSRP+DG+VVLTPRVDG+DT+PSHRGNHF I RRS EIFNSEVV
Sbjct: 301 FIASESKFTRFNFYLDVSRPQDGIVVLTPRVDGVDTFPSHRGNHFLIRRRSAEIFNSEVV 360

Query: 361 ACPLDNTSATTVILPHRESVKIQEIELFLNHIVILEREDGLPKVVVYSLPDIGEPLKTLE 420
           ACPLDNTSAT VILPHRESVKIQ+I+LFLNHIV+ EREDGLPK+VVYSLPDIGEPL++LE
Sbjct: 361 ACPLDNTSATEVILPHRESVKIQDIQLFLNHIVVFEREDGLPKIVVYSLPDIGEPLRSLE 420

Query: 421 GGRAVDFADATYSVDALESEFSSSILRFCYSSMKTPPSTYDYDMKTGVSVLKKVETVLGG 480
           GGRAVDF DATYSV   +SEFSS+ILRFCYSSM+TPPSTYDYDMKTGVS+LKKVE VLGG
Sbjct: 421 GGRAVDFTDATYSVYLSDSEFSSTILRFCYSSMRTPPSTYDYDMKTGVSILKKVEAVLGG 480

Query: 481 FDSNKYVTERKWATALDGTKVPLSIVYRKDLVKLDGSDPLLLYGYGSYEYCIDPSFKASR 540
           FD   YVTERKWATALDGTKVPLSI YRKDLVKLDGSDPLLLYGYGSYE C+DPSFK SR
Sbjct: 481 FDITNYVTERKWATALDGTKVPLSIAYRKDLVKLDGSDPLLLYGYGSYEACVDPSFKGSR 540

Query: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFISSAEYLIENKYCSKEKLC 600
           ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFI+SAEYLIENKYCSKEKLC
Sbjct: 541 ISLLDRGFIYVIAHIRGGGEMGRQWYENGKLLKKKNTFTDFIASAEYLIENKYCSKEKLC 600

Query: 601 INGRSAGGLLIGAVINMRPDLFKAAIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660
           INGRSAGGLLIGAV+NMRPDLFKAA+AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE
Sbjct: 601 INGRSAGGLLIGAVLNMRPDLFKAAVAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEE 660

Query: 661 FYFYMKSYSPVDNVKAQNYPDVLITAGLN 689
           FYFYMKSYSPVDNVKAQ+YPD+L+TAGLN
Sbjct: 661 FYFYMKSYSPVDNVKAQDYPDILVTAGLN 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O078344.2e-13039.85Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
Q595361.1e-12738.38Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
P245552.5e-11939.06Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
P556568.8e-8031.17Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
P556276.1e-7330.06Uncharacterized peptidase y4qF OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
XP_004147101.10.0100.00uncharacterized protein LOC101213609 [Cucumis sativus] >KGN51571.1 hypothetical ... [more]
XP_008445904.10.095.94PREDICTED: protease 2 [Cucumis melo] >KAA0034088.1 protease 2 [Cucumis melo var.... [more]
XP_038893092.10.091.73protease 2 [Benincasa hispida][more]
XP_023541732.10.089.40uncharacterized protein LOC111801801 [Cucurbita pepo subsp. pepo][more]
KAG6573758.10.088.97Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KV300.0100.00Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G579570 PE=3 SV=1[more]
A0A5A7STX70.095.94Prolyl endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
A0A1S3BDS70.095.94Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103488786 PE=3 SV=1[more]
A0A6J1G0210.088.97Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111449474 PE=3 SV=1[more]
A0A6J1HU740.088.68Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111466641 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 52..378
e-value: 5.4E-85
score: 285.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 71..121
e-value: 1.4E-77
score: 263.7
NoneNo IPR availableGENE3D2.130.10.120coord: 122..378
e-value: 1.4E-77
score: 263.7
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 46..378
NoneNo IPR availablePANTHERPTHR11757:SF18BNAA08G02180D PROTEINcoord: 46..378
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 48..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10036.T1Cucsat.G10036.T1mRNA
Cucsat.G10036.T2Cucsat.G10036.T2mRNA
Cucsat.G10036.T5Cucsat.G10036.T5mRNA
Cucsat.G10036.T13Cucsat.G10036.T13mRNA
Cucsat.G10036.T10Cucsat.G10036.T10mRNA
Cucsat.G10036.T8Cucsat.G10036.T8mRNA
Cucsat.G10036.T3Cucsat.G10036.T3mRNA
Cucsat.G10036.T4Cucsat.G10036.T4mRNA
Cucsat.G10036.T6Cucsat.G10036.T6mRNA
Cucsat.G10036.T7Cucsat.G10036.T7mRNA
Cucsat.G10036.T9Cucsat.G10036.T9mRNA
Cucsat.G10036.T11Cucsat.G10036.T11mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity