Cucsat.G10024.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G10024.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cysteine proteinase inhibitor
Location	ctg1673: 3427254 .. 3430436 (+)
RNA-Seq Expression	Cucsat.G10024.T2
Synteny	Cucsat.G10024.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGGTTGACTGTGGTATGATTATTATTTCTTAGAAGGAACTTGACTGTCTATGGTTTTTAGATTTGGACAGTGGATATGCTTAAAATGTGTTTTTAGATTGACCATAGAATTTGAATTCGCGTCTAAAAGTTCGAAAAACTGAACCAGTCTTTCTGGCTATCAATAGTTAGGATTAACATTCAATTTTTATTATGAAAATCTTGCAATCTTCCATAAGATGATAACTGGAAACATATTGGCGTTTTAATCTTTTCAAGGCTGATGGAGACCAGGTTAGCGAATGGTCCCTATGACGATGTGATTTTCTATTTATCTTCTGATCACTTCTAGTTACTTAAACAAGCACGTTTCCATGTCTTTAGAAGAGAAGTTTGTTTCTTCGTTTCTCACAAGTGTTTCATGTTAATTTTTTTTTCAGTTTAAACACTCTATCTATGAAATCCCAACTAATTGTATATTGATTTTATTTTAGTATAAGAACTCCTTTTACTATCAAATCCAATGTATATGTATACTAACTTCATTTCTGTTTAAAAGATTAGTTTTCTCTCATTTCTGAGTATTTTGTATTGAAGTTTTATTTCAGCTTTTAAGTGGAAGTTCTATTTTAATTTCATGGACTCTTTTTCCATAACTAAGGAGGAAACTTTCCTTTGACAAATATCCGCAATAAATTCGTTGGAAATTTCCAAATTGGACACACATAAGCATGGTATTGTAGTTGTTTGCAACTAAAAATTCCTCCTGTTCCTATTTCCTCTTTTCATGCAGCAGACGTATAACTCATATGTTATTTATGTGTATGATGTAATTATCTTCCCATTCCTTTGCAGAATTCTCTTCTTGAGTTTGTGAGAGTTGTGAAGGCAAAAGAGCAGGTAGTTGCTGGTACATTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGAATGACATTCTTTAACTCCTGCAGTGCAGTTTCAATTACAATTTAAAGTATAGCTTTGAATATAGAACCAGTGTCAAAGGAAATGTGTTGACTCATATTCTACCTGTTTTTTTGGAGATTATTTTCAGTTGAAGTCATTCATAACCTCTTGAAGACTGATAAAATCTGAAGTTGTGGCTTTGTTTCTTTTCTAATTTACTAAGGGTTGATATTCTTGTGAACTTGCATTGTCAACTATTTAGCAAATGTTAGTAGCTCTATTATAGAGCATAAGCATTCATTAGTTCTTGTATGTTCGTTTGTATTCTTACAGGTTAAAAAGCTGTAAATGTATGTGAAAGTCTTGTTACTGATTTTACAGACAATTGTTTTCTTTAATATCCTACTGACCATGGTTAGAAATTAGGTGATCATCCCCAGGGATGGCGAGAAGTGGCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCATATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTAAGTGGGTTATTGTCCTTTTATGTTGTATGTTTTTACCTCTGTTTAAGTTGGTTATCATGAGACTGTGAAGTTGATCTATGATATCAGGATTTGTCTTTTTCATTGATGATGAAATAGGACTAAATTCACATCATTGAAAGGTGGATCATAAAAGAGCCCTCCAACTAGAAATCAACAAAGAAATACTTTAGTCATTAAAAGGCTGTTTACATTGACACCAATACAATAAAACGTATAAAATGAAACATGTGTGTGCTTTGAGTTTTTGTTTGTAGCAAACCCCACCGTTGAAAGGGCATTCCACAGAGACGAAAGCTTTATTATCCTAAATGTTGTTTGGAAAAGACATGGAACATGTGTGTGCTCTGAGTTAGAAAAACACATCAAGATAGAGAGAGGTTGGAATGAGGCTTACATTATAGAGAATGAAATAGCTGCAAGACTTAGCTTCCCGTAGAAAGATCTTTATTTATTTACATGAAAGAAAAACAAGGGCATACAAAAAACTAAATCCGCAAAATTCCTACAAAAGGAGAGGGGTTCCAACTAGATAAAACCTTACCTAAAACATCTGTATATTCTTTGTTCCTTTATAATTTAGACATGTTTGATTGACCAGAAAGTAAAGGTAGCATTCATCCCAGAAGGCATTAAGTTTGAACTTTTGTAGTATTCGTTGTGTTTCCATAATTTGTTTTAAAGCTTTTATGTTAATATCATTTTGATTAGCTTAACTTCTAATCTACCACTTCTATCAAAATTAATACGACCAACATTTTAAACCCGAGAAGGTAGGGCATTCATTCATACCCCATGTTCACACTTTATGAAAATTTTCTTCTGGTGAAAACTCAACATCCTAAATCACATACCTTTTGTTCATTTTAATTATGTTGTTTATCAATTTACATAATACTAATGAGAGATTCATGATGGCGTGAGAACAGGTGATCGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAAGGGAGTTTGGATGGTTCAATATAATTAAATAAAATACAAGTTTATGTACAATATAACCTTTAAAGCTGTATACTTCTTAACTTCTTTTGAAGTTCGAGTCGTCAGGCTGATACATAAAAAAAACCATGGTCAATGATGTAGCCACTTACTACTTAGGTTTTGGATCTGTGGTTTCCTTTTCATCTGCTTTCTTTAATCCTTGCTTGAATTGTATGAACAACAAAACTAAACAAAACATAGGGATTGTGGGTATATTTTCAATTCTGGGTTTACACTATGAAGTGAACCTGTTCCTTTTCGTTTTCGACCTAGTTTTAGAAAGTCCTTTAAAAATCTCACACAGATATGAGAGTTAATGTGGATTTAAGCTTAATTTGGGATTGAAAATTAGTATCATGTGGTGTGTTTATCAAATATAATCTATCGTACTTGAAGTGTATGGCTTGGTGTTGTCTATCGACACACTAAACACCATTTTCAAGACTT

Coding sequence (CDS)

ATGAATTCTCTTCTTGAGTTTGTGAGAGTTGTGAAGGCAAAAGAGCAGGTAGTTGCTGGTACATTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGATCATCCCCAGGGATGGCGAGAAGTGGCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCATATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTGATCGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAA

Protein sequence

MNSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS

Homology

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.5e-65
Identity = 121/166 (72.89%), Postives = 145/166 (87.35%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           N+LLEFVRVV+AKEQVVAGTLHHLT+E +EAG+KK+YEAKVWVK W++FKELQEF++ GD
Sbjct: 79  NALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQEFRNTGD 138

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
             + T +DLGAKKG H  GWR+V  HDP V+DAA HA+++IQQRSNSL PYELLEI+ AK
Sbjct: 139 ATTFTNADLGAKKGGHEPGWRDVPVHDPVVKDAADHAVKSIQQRSNSLFPYELLEIVRAK 198

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDH 168
           AEV+ED AKFD+L+KLKRG+KEEKFK EVHKN EG F+LNQM Q+H
Sbjct: 199 AEVVEDFAKFDILMKLKRGNKEEKFKAEVHKNLEGAFVLNQMQQEH 244

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 6.8e-63
Identity = 119/166 (71.69%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           N+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQEFK A D
Sbjct: 68  NALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKPASD 127

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
            P+IT SDLG K+G+H  GWREV   DP V+  A+ A++TIQQRSNSL PYELLE++HAK
Sbjct: 128 APAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFPYELLEVVHAK 187

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDH 168
           AEV  +AAK+++LLKLKRG KEEKFKVEVHKN+EG   LN   Q H
Sbjct: 188 AEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLNHAEQHH 233

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 2.2e-45
Identity = 92/157 (58.60%), Postives = 126/157 (80.25%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           N++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNFK+LQEFK+   
Sbjct: 77  NAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFKNI-- 136

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           +PS T SDLG K   +   WR V+ ++P VQ+AA+HA++++QQ+SNSL PY+L++II A+
Sbjct: 137 IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPYKLIDIILAR 196

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 159
           A+V+E+  KF+LLLKL+RG+K EKF VEV K+  G +
Sbjct: 197 AKVVEERVKFELLLKLERGNKLEKFMVEVMKDQTGKY 231

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 41/63 (65.08%), Postives = 53/63 (84.13%), Query Frame = 0


Query: 2  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
          N+LLEF RVV A++QVV+GTL+ +T+E  + G+KK+YEAKVW K W+NFKELQEFKH GD
Sbjct: 35 NALLEFGRVVSAQQQVVSGTLYTITLEAKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGD 94

Query: 62 VPS 65
           P+
Sbjct: 95 APA 97

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot
Match: P37842 (Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.8e-16
Identity = 50/143 (34.97%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           N+ LEFV  +  KEQ+VAG L+++T+  ++AGKKK+YEAK+WVK W NFK++ EFK  GD
Sbjct: 506 NAHLEFVENLNVKEQLVAGMLYYITLVAIDAGKKKIYEAKIWVKEWENFKKVIEFKLIGD 565

Query: 62  VPSITPSDLGAKKGDHPQGWREVA-PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHA 121
             +I              G+ +V  P++P  QD A+ A++   ++ N+ + Y  +E ++ 
Sbjct: 566 DSAII------------GGFTDVPFPNNPEFQDLARFAVQDYNKKENAHLEY--VENLNV 625

Query: 122 KAEVIEDAAKFDLLLKLKRGSKE 144
           K +++     +  L+    G K+
Sbjct: 626 KEQLVAGMIYYITLVATDAGKKK 634

[Close]

BLAST of Cucsat.G10024.T2 vs. NCBI nr
Match: XP_004147084.1 (cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical protein Csa_008462 [Cucumis sativus])

HSP 1 Score: 331 bits (848), Expect = 5.37e-113
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 83  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 142

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 143 VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 202

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 203 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 249

[Close]

BLAST of Cucsat.G10024.T2 vs. NCBI nr
Match: XP_008445944.1 (PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 327 bits (839), Expect = 1.30e-111
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 84  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 143

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 144 VPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 203

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 204 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 250

[Close]

BLAST of Cucsat.G10024.T2 vs. NCBI nr
Match: KAA0034127.1 (cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 327 bits (839), Expect = 1.30e-111
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 84  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 143

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 144 VPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 203

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 204 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 250

[Close]

BLAST of Cucsat.G10024.T2 vs. NCBI nr
Match: XP_038891963.1 (cysteine proteinase inhibitor 12 [Benincasa hispida])

HSP 1 Score: 319 bits (817), Expect = 2.55e-108
Identity = 159/167 (95.21%), Postives = 165/167 (98.80%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NS+LEFVRVVKAKEQVVAGTLHHLT+EVV+AGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 80  NSILEFVRVVKAKEQVVAGTLHHLTLEVVDAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 139

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSIT SDLGAKKGDHPQGWREV PHDPHVQDAAQHA++TIQQRSNSL+PYELLEIIHAK
Sbjct: 140 VPSITSSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAVQTIQQRSNSLLPYELLEIIHAK 199

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 200 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 246

[Close]

BLAST of Cucsat.G10024.T2 vs. NCBI nr
Match: XP_022957234.1 (cysteine proteinase inhibitor 12-like [Cucurbita moschata])

HSP 1 Score: 314 bits (805), Expect = 1.60e-106
Identity = 156/167 (93.41%), Postives = 161/167 (96.41%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYEAK+WVKSWMNFKELQEFKHAGD
Sbjct: 78  NSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWVKSWMNFKELQEFKHAGD 137

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
            PS +PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSL+PYELLEI+HAK
Sbjct: 138 APSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLLPYELLEIVHAK 197

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV D S
Sbjct: 198 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVPDQS 244

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL
Match: A0A0A0KV53 (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV=1)

HSP 1 Score: 331 bits (848), Expect = 2.60e-113
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 83  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 142

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 143 VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 202

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 203 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 249

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL
Match: A0A5D3CW86 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002960 PE=3 SV=1)

HSP 1 Score: 327 bits (839), Expect = 6.32e-112
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 84  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 143

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 144 VPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 203

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 204 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 250

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL
Match: A0A5A7SXY4 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001180 PE=3 SV=1)

HSP 1 Score: 327 bits (839), Expect = 6.32e-112
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 84  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 143

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 144 VPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 203

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 204 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 250

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL
Match: A0A1S3BEK2 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1)

HSP 1 Score: 327 bits (839), Expect = 6.32e-112
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD
Sbjct: 84  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 143

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
           VPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK
Sbjct: 144 VPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 203

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS
Sbjct: 204 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 250

[Close]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL
Match: A0A6J1H1C9 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3 SV=1)

HSP 1 Score: 314 bits (805), Expect = 7.73e-107
Identity = 156/167 (93.41%), Postives = 161/167 (96.41%), Query Frame = 0


Query: 2   NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGD 61
           NSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYEAK+WVKSWMNFKELQEFKHAGD
Sbjct: 78  NSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWVKSWMNFKELQEFKHAGD 137

Query: 62  VPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAK 121
            PS +PSDLGAKKGDHPQGWREV PHDPHVQDAAQHALRTIQQRSNSL+PYELLEI+HAK
Sbjct: 138 APSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQRSNSLLPYELLEIVHAK 197

Query: 122 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS 168
           AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV D S
Sbjct: 198 AEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVPDQS 244

The following BLAST results are available for this feature:

BLAST of Cucsat.G10024.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0JNR2	2.5e-65	72.89	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q8H0X6	6.8e-63	71.69	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q8LC76	2.2e-45	58.60	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]
Q06445	1.1e-17	65.08	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
P37842	4.8e-16	34.97	Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1	[more]

BLAST of Cucsat.G10024.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147084.1	5.37e-113	100.00	cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical prot...	[more]
XP_008445944.1	1.30e-111	98.80	PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine ...	[more]
KAA0034127.1	1.30e-111	98.80	cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa]	[more]
XP_038891963.1	2.55e-108	95.21	cysteine proteinase inhibitor 12 [Benincasa hispida]	[more]
XP_022957234.1	1.60e-106	93.41	cysteine proteinase inhibitor 12-like [Cucurbita moschata]	[more]

BLAST of Cucsat.G10024.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV53	2.60e-113	100.00	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV...	[more]
A0A5D3CW86	6.32e-112	98.80	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A5A7SXY4	6.32e-112	98.80	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A1S3BEK2	6.32e-112	98.80	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1	[more]
A0A6J1H1C9	7.73e-107	93.41	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 2..59 e-value: 0.0028 score: 26.9
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 3..58 e-value: 4.6E-12 score: 46.1
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 5..58 e-value: 4.36171E-13 score: 60.1804
None	No IPR available	GENE3D	3.10.450.10		coord: 1..66 e-value: 9.4E-26 score: 92.0
None	No IPR available	GENE3D	3.10.450.10		coord: 72..165 e-value: 1.3E-10 score: 43.3
None	No IPR available	PANTHER	PTHR11413:SF103	CYSTEINE PROTEINASE INHIBITOR 6	coord: 2..164
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 2..62
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 73..160
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 2..164
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 15..28

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G10024	Cucsat.G10024	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10024.T2.E1	Cucsat.G10024.T2.E1	exon
Cucsat.G10024.T2.E2	Cucsat.G10024.T2.E2	exon
Cucsat.G10024.T2.E3	Cucsat.G10024.T2.E3	exon
Cucsat.G10024.T2.E4	Cucsat.G10024.T2.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10024.T2.C1	Cucsat.G10024.T2.C1	CDS
Cucsat.G10024.T2.C2	Cucsat.G10024.T2.C2	CDS
Cucsat.G10024.T2.C3	Cucsat.G10024.T2.C3	CDS
Cucsat.G10024.T2.C4	Cucsat.G10024.T2.C4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G10024.T2	Cucsat.G10024.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity