Cucsat.G10024 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G10024
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cysteine proteinase inhibitor
Location	ctg1673: 3426284 .. 3430400 (+)
RNA-Seq Expression	Cucsat.G10024
Synteny	Cucsat.G10024

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGATCCTCACATTGATGAGTAAAAATCAGTTAATTATCGATTCAAAACTCTTTAAAATCGATCGAGTTTGTTTTTCAATTGATCGAGTTTGATTTAATTTGTGGAATTTTGTTTTTCAATTGATTGACTTTGATTTAATTGGTGGACTCGGATTTTGGATCTAACATACTCGAATGTACATAAGACGGTGTCTTGATATCTAGACGTTGAGAGTGCAATTTGACTTCCAATAAACCTATTGAAGATTCAAAAGAAATTTGATTCTTTGCCCAATAAATAAAATGCTTAAACCAAACGCATCGCTTCGTGGCGCAGTTTCTTAGTTGGCACGAATACCCATTTTACGATCGTGGCGCCAAGGGAGATAGAGATAGAGAGAGAGATTCTCATTCATATCTCACCGCGTAGAAGTTTTTGACAAAACCGAAAGACATGAAAGGAACTGCGAACATCTCTCCTCTCTGATGAACAAGGATACTTCCATTTCGTTTTCTCCATTATCATCTGCTTTGTTGTTGTTGCTGCTGCTGTTTTTCTCTGGGATCATTGTTCTTGCAGGTAACGCATCAGCTCACTTTTGCGCTGACCAGGACGATCCTCTCCTTATGGCGTCCACACTTGGAGACGTTCGCGATTCTCAGGGAGCTTCCAACTCCGCCGATGTTGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGGTAAACAGCTTCTCTCTCGATATCTATCTAAGTGAATTTTTTTCTTACTCCGTGTTTTTTATTCTGTCACGTTTCGTTGATTCTCGCTGATGGCGTCTGTGCTTTTTGTTGTCAATATTGTTCTTGTTGTGTGTTGTGCTGTGGATTTCTGTGTTTCAGGCAGAATTCATTTCGCGATTTTGAATTTTGTTTGACTTGTTCTGCATTTTGAGGCTGTGGGTTTCTGTCGGTGTCGATTAGTATGAGGATGTGTGTTGAACTAAAACTGCACGATTCTATTTGTCTTTCTTTGTTCGATAGATTTGGATCGGTTGACTGTGGTATGATTATTATTTCTTAGAAGGAACTTGACTGTCTATGGTTTTTAGATTTGGACAGTGGATATGCTTAAAATGTGTTTTTAGATTGACCATAGAATTTGAATTCGCGTCTAAAAGTTCGAAAAACTGAACCAGTCTTTCTGGCTATCAATAGTTAGGATTAACATTCAATTTTTATTATGAAAATCTTGCAATCTTCCATAAGATGATAACTGGAAACATATTGGCGTTTTAATCTTTTCAAGGCTGATGGAGACCAGGTTAGCGAATGGTCCCTATGACGATGTGATTTTCTATTTATCTTCTGATCACTTCTAGTTACTTAAACAAGCACGTTTCCATGTCTTTAGAAGAGAAGTTTGTTTCTTCGTTTCTCACAAGTGTTTCATGTTAATTTTTTTTTCAGTTTAAACACTCTATCTATGAAATCCCAACTAATTGTATATTGATTTTATTTTAGTATAAGAACTCCTTTTACTATCAAATCCAATGTATATGTATACTAACTTCATTTCTGTTTAAAAGATTAGTTTTCTCTCATTTCTGAGTATTTTGTATTGAAGTTTTATTTCAGCTTTTAAGTGGAAGTTCTATTTTAATTTCATGGACTCTTTTTCCATAACTAAGGAGGAAACTTTCCTTTGACAAATATCCGCAATAAATTCGTTGGAAATTTCCAAATTGGACACACATAAGCATGGTATTGTAGTTGTTTGCAACTAAAAATTCCTCCTGTTCCTATTTCCTCTTTTCATGCAGCAGACGTATAACTCATATGTTATTTATGTGTATGATGTAATTATCTTCCCATTCCTTTGCAGAATTCTCTTCTTGAGTTTGTGAGAGTTGTGAAGGCAAAAGAGCAGGTAGTTGCTGGTACATTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGAATGACATTCTTTAACTCCTGCAGTGCAGTTTCAATTACAATTTAAAGTATAGCTTTGAATATAGAACCAGTGTCAAAGGAAATGTGTTGACTCATATTCTACCTGTTTTTTTGGAGATTATTTTCAGTTGAAGTCATTCATAACCTCTTGAAGACTGATAAAATCTGAAGTTGTGGCTTTGTTTCTTTTCTAATTTACTAAGGGTTGATATTCTTGTGAACTTGCATTGTCAACTATTTAGCAAATGTTAGTAGCTCTATTATAGAGCATAAGCATTCATTAGTTCTTGTATGTTCGTTTGTATTCTTACAGGTTAAAAAGCTGTAAATGTATGTGAAAGTCTTGTTACTGATTTTACAGACAATTGTTTTCTTTAATATCCTACTGACCATGGTTAGAAATTAGGTGATCATCCCCAGGGATGGCGAGAAGTGGCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCATATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTAAGTGGGTTATTGTCCTTTTATGTTGTATGTTTTTACCTCTGTTTAAGTTGGTTATCATGAGACTGTGAAGTTGATCTATGATATCAGGATTTGTCTTTTTCATTGATGATGAAATAGGACTAAATTCACATCATTGAAAGGTGGATCATAAAAGAGCCCTCCAACTAGAAATCAACAAAGAAATACTTTAGTCATTAAAAGGCTGTTTACATTGACACCAATACAATAAAACGTATAAAATGAAACATGTGTGTGCTTTGAGTTTTTGTTTGTAGCAAACCCCACCGTTGAAAGGGCATTCCACAGAGACGAAAGCTTTATTATCCTAAATGTTGTTTGGAAAAGACATGGAACATGTGTGTGCTCTGAGTTAGAAAAACACATCAAGATAGAGAGAGGTTGGAATGAGGCTTACATTATAGAGAATGAAATAGCTGCAAGACTTAGCTTCCCGTAGAAAGATCTTTATTTATTTACATGAAAGAAAAACAAGGGCATACAAAAAACTAAATCCGCAAAATTCCTACAAAAGGAGAGGGGTTCCAACTAGATAAAACCTTACCTAAAACATCTGTATATTCTTTGTTCCTTTATAATTTAGACATGTTTGATTGACCAGAAAGTAAAGGTAGCATTCATCCCAGAAGGCATTAAGTTTGAACTTTTGTAGTATTCGTTGTGTTTCCATAATTTGTTTTAAAGCTTTTATGTTAATATCATTTTGATTAGCTTAACTTCTAATCTACCACTTCTATCAAAATTAATACGACCAACATTTTAAACCCGAGAAGGTAGGGCATTCATTCATACCCCATGTTCACACTTTATGAAAATTTTCTTCTGGTGAAAACTCAACATCCTAAATCACATACCTTTTGTTCATTTTAATTATGTTGTTTATCAATTTACATAATACTAATGAGAGATTCATGATGGCGTGAGAACAGGTGATCGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAAGGGAGTTTGGATGGTTCAATATAATTAAATAAAATACAAGTTTATGTACAATATAACCTTTAAAGCTGTATACTTCTTAACTTCTTTTGAAGTTCGAGTCGTCAGGCTGATACATAAAAAAAACCATGGTCAATGATGTAGCCACTTACTACTTAGGTTTTGGATCTGTGGTTTCCTTTTCATCTGCTTTCTTTAATCCTTGCTTGAATTGTATGAACAACAAAACTAAACAAAACATAGGGATTGTGGGTATATTTTCAATTCTGGGTTTACACTATGAAGTGAACCTGTTCCTTTTCGTTTTCGACCTAGTTTTAGAAAGTCCTTTAAAAATCTCACACAGATATGAGAGTTAATGTGGATTTAAGCTTAATTTGGGATTGAAAATTAGTATCATGTGGTGTGTTTATCAAATATAATCTATCGTACTTGAAGTGTATGGCTTGGTGTTGTCTATCGACACACTAAACACCATTTTCAAGACTTCAGACTTCTCTCAACAACAAATC

Coding sequence (CDS)

ATGAACAAGGATACTTCCATTTCGTTTTCTCCATTATCATCTGCTTTGTTGTTGTTGCTGCTGCTGTTTTTCTCTGGGATCATTGTTCTTGCAGGTAACGCATCAGCTCACTTTTGCGCTGACCAGGACGATCCTCTCCTTATGGCGTCCACACTTGGAGACGTTCGCGATTCTCAGGGAGCTTCCAACTCCGCCGATGTTGATGAACTCGCTCGTTTTGCCGTCGAGGAACACAACAAGAAAGAGAATTCTCTTCTTGAGTTTGTGAGAGTTGTGAAGGCAAAAGAGCAGGTAGTTGCTGGTACATTACACCATCTTACTGTTGAGGTTGTTGAGGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGTCATGGATGAATTTTAAAGAATTGCAAGAGTTTAAGCATGCAGGCGATGTCCCCTCAATTACTCCTTCTGATCTTGGTGCAAAAAAAGGTGATCATCCCCAGGGATGGCGAGAAGTGGCACCACATGATCCTCATGTTCAGGATGCAGCACAGCATGCTCTTCGAACCATCCAGCAGAGATCTAATTCTCTAGTCCCATATGAACTGCTGGAGATCATTCATGCCAAGGCAGAGGTGATCGAAGATGCTGCAAAGTTTGATTTGCTCCTAAAGCTGAAGAGAGGGAGTAAAGAAGAGAAGTTCAAAGTGGAGGTCCACAAGAACAATGAAGGTAACTTCCTTCTAAATCAGATGGTGCAAGATCATTCCTAA

Protein sequence

MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQGASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMVQDHS

Homology

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BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 2.3e-58
Identity = 119/197 (60.41%), Postives = 152/197 (77.16%), Query Frame = 0


Query: 18  LLLLLFFSGIIVLAGNASAH--FCADQDDPLLMASTLGDVRDSQGASNSADVDELARFAV 77
           LL ++FFS  + ++   ++   FC   ++ + +   +GDV  +Q   NS +V+ LARFAV
Sbjct: 7   LLFIVFFSLSLFISSLIASDLGFC---NEEMALVGGVGDVPANQ---NSGEVESLARFAV 66

Query: 78  EEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQ 137
           +EHNKKEN+LLEF RVVKAKEQVVAGTLHHLT+E++EAG+KKLYEAKVWVK W+NFKELQ
Sbjct: 67  DEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQ 126

Query: 138 EFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPYEL 197
           EFK A D P+IT SDLG K+G+H  GWREV   DP V+  A+ A++TIQQRSNSL PYEL
Sbjct: 127 EFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFPYEL 186

Query: 198 LEIIHAKAEVSGLLSFY 213
           LE++HAKAEV+G  + Y
Sbjct: 187 LEVVHAKAEVTGEAAKY 197

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BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 8.8e-58
Identity = 117/195 (60.00%), Postives = 146/195 (74.87%), Query Frame = 0


Query: 11  PLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQGASNSADVDEL 70
           P SS+    L LF   ++ +A  A+A F        +    LG   D+  A+NS + D L
Sbjct: 9   PASSSAAAPLPLFL--LLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDAL 68

Query: 71  ARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMN 130
           ARFAV+EHNK+EN+LLEFVRVV+AKEQVVAGTLHHLT+E +EAG+KK+YEAKVWVK W++
Sbjct: 69  ARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLD 128

Query: 131 FKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSL 190
           FKELQEF++ GD  + T +DLGAKKG H  GWR+V  HDP V+DAA HA+++IQQRSNSL
Sbjct: 129 FKELQEFRNTGDATTFTNADLGAKKGGHEPGWRDVPVHDPVVKDAADHAVKSIQQRSNSL 188

Query: 191 VPYELLEIIHAKAEV 206
            PYELLEI+ AKAEV
Sbjct: 189 FPYELLEIVRAKAEV 201

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BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 3.0e-42
Identity = 91/192 (47.40%), Postives = 139/192 (72.40%), Query Frame = 0


Query: 18  LLLLLFFSGIIVLAGNASAHFCADQD---DPLLMASTLGDVRDSQGASNSA-DVDELARF 77
           + ++L    +++L+G      C+++    +  ++   LG   DS+   N   ++D++A F
Sbjct: 8   MCMMLICVSLVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIALF 67

Query: 78  AVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKE 137
           AV+EHN++EN++LE  RV+KA EQVVAG L+ LT+EV+EAG+KK+YEAKVWVK WMNFK+
Sbjct: 68  AVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQ 127

Query: 138 LQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHALRTIQQRSNSLVPY 197
           LQEFK+   +PS T SDLG K   +   WR V+ ++P VQ+AA+HA++++QQ+SNSL PY
Sbjct: 128 LQEFKNI--IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPY 187

Query: 198 ELLEIIHAKAEV 206
           +L++II A+A+V
Sbjct: 188 KLIDIILARAKV 197

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BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-28
Identity = 61/96 (63.54%), Postives = 77/96 (80.21%), Query Frame = 0


Query: 50  STLGDVRDSQGASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVE 109
           + LG  RD  G  NS ++D LARFAVEEHNKK+N+LLEF RVV A++QVV+GTL+ +T+E
Sbjct: 2   AALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLE 61

Query: 110 VVEAGKKKLYEAKVWVKSWMNFKELQEFKHAGDVPS 146
             + G+KK+YEAKVW K W+NFKELQEFKH GD P+
Sbjct: 62  AKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGDAPA 97

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BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-26
Identity = 57/118 (48.31%), Postives = 81/118 (68.64%), Query Frame = 0


Query: 23  FFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQGASNSADVDELARFAVEEHNKKE 82
           F+   ++L G      C  ++        LG V D +G  NS +++ LARFA++EHNK++
Sbjct: 6   FWIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQ 65

Query: 83  NSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYEAKVWVKSWMNFKELQEFKHA 141
           N +LEF ++VKA+EQVVAGT++HLT+E  E  + K +EAKVWVK WMNFK+LQEFK +
Sbjct: 66  NKILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

[Close]

BLAST of Cucsat.G10024 vs. NCBI nr
Match: XP_004147084.1 (cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical protein Csa_008462 [Cucumis sativus])

HSP 1 Score: 399 bits (1026), Expect = 3.99e-139
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60
           MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG
Sbjct: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60

Query: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE
Sbjct: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205
           RTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205

[Close]

BLAST of Cucsat.G10024 vs. NCBI nr
Match: XP_008445944.1 (PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 372 bits (954), Expect = 3.83e-128
Identity = 193/206 (93.69%), Postives = 197/206 (95.63%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVLAGNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 205
           LRTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 206

[Close]

BLAST of Cucsat.G10024 vs. NCBI nr
Match: KAA0034127.1 (cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa])

HSP 1 Score: 370 bits (950), Expect = 1.56e-127
Identity = 192/206 (93.20%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVL GNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 205
           LRTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 206

[Close]

BLAST of Cucsat.G10024 vs. NCBI nr
Match: XP_038891963.1 (cysteine proteinase inhibitor 12 [Benincasa hispida])

HSP 1 Score: 352 bits (903), Expect = 1.94e-120
Identity = 182/205 (88.78%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60
           MNK++SIS   LSS++ LLLLL FS IIVLAGNASA FCA QDDPLLMASTLG VRDSQG
Sbjct: 1   MNKNSSIS---LSSSIALLLLLLFSWIIVLAGNASAQFCAQQDDPLLMASTLGGVRDSQG 60

Query: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNS DVDELARFAV+EHNKKENS+LEFVRVVKAKEQVVAGTLHHLT+EVV+AGKKKLYE
Sbjct: 61  ASNSTDVDELARFAVDEHNKKENSILEFVRVVKAKEQVVAGTLHHLTLEVVDAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVPSIT SDLGAKKGDHPQGWREV PHDPHVQDAAQHA+
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITSSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAV 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205
           +TIQQRSNSL+PYELLEIIHAKAEV
Sbjct: 181 QTIQQRSNSLLPYELLEIIHAKAEV 202

[Close]

BLAST of Cucsat.G10024 vs. NCBI nr
Match: XP_023548422.1 (cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343 bits (881), Expect = 4.05e-117
Identity = 179/205 (87.32%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60
           M  D+SI FS  SS LLLLLLL +   IVLAGNASAHFCA QD+PLLMASTLG V DSQG
Sbjct: 1   MKNDSSIWFS--SSTLLLLLLLSW---IVLAGNASAHFCAQQDNPLLMASTLGGVHDSQG 60

Query: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNSADVDELARFAV+EHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYE
Sbjct: 61  ASNSADVDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180
           AK+WVKSWMNFKELQEFKHAGD PS +PSDLGAKKGDHPQGWREV PHDPHVQDAAQHAL
Sbjct: 121 AKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205
           RTIQQRSNSL+PYELLEI+HAKAEV
Sbjct: 181 RTIQQRSNSLLPYELLEIVHAKAEV 200

[Close]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL
Match: A0A0A0KV53 (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV=1)

HSP 1 Score: 399 bits (1026), Expect = 1.93e-139
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60
           MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG
Sbjct: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60

Query: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE
Sbjct: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180
           AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL
Sbjct: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205
           RTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205

[Close]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL
Match: A0A5D3CW86 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002960 PE=3 SV=1)

HSP 1 Score: 372 bits (954), Expect = 1.86e-128
Identity = 193/206 (93.69%), Postives = 197/206 (95.63%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVLAGNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 205
           LRTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 206

[Close]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL
Match: A0A1S3BEK2 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1)

HSP 1 Score: 372 bits (954), Expect = 1.86e-128
Identity = 193/206 (93.69%), Postives = 197/206 (95.63%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVLAGNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 205
           LRTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 206

[Close]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL
Match: A0A5A7SXY4 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001180 PE=3 SV=1)

HSP 1 Score: 370 bits (950), Expect = 7.55e-128
Identity = 192/206 (93.20%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSAL-LLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQ 60
           MN  ++ISFSPLSS L LLLLLLFFS IIVL GNASAHFCA+QDDPLLMASTLG V DSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLTGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120
           GASNS DVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHA 180
           EAKVWVKSWMNFKELQEFKHAGDVPSI+PSDLGAKKGDHPQGWREV PHDPHVQDAAQHA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 205
           LRTIQQRSNSLVPYELLEIIHAKAEV
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEV 206

[Close]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL
Match: A0A6J1H1C9 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3 SV=1)

HSP 1 Score: 342 bits (877), Expect = 7.97e-117
Identity = 178/205 (86.83%), Postives = 186/205 (90.73%), Query Frame = 0


Query: 1   MNKDTSISFSPLSSALLLLLLLFFSGIIVLAGNASAHFCADQDDPLLMASTLGDVRDSQG 60
           M  D+SI FS  SS  LLLLLL +   IVLAGNASAHFCA QDDPLLMASTLG V DSQG
Sbjct: 1   MKNDSSIWFS--SSTFLLLLLLSW---IVLAGNASAHFCAQQDDPLLMASTLGGVHDSQG 60

Query: 61  ASNSADVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLYE 120
           ASNSAD DELARFAV+EHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEV +AGKKKLYE
Sbjct: 61  ASNSADFDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYE 120

Query: 121 AKVWVKSWMNFKELQEFKHAGDVPSITPSDLGAKKGDHPQGWREVAPHDPHVQDAAQHAL 180
           AK+WVKSWMNFKELQEFKHAGD PS +PSDLGAKKGDHPQGWREV PHDPHVQDAAQHAL
Sbjct: 121 AKIWVKSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHAL 180

Query: 181 RTIQQRSNSLVPYELLEIIHAKAEV 205
           RTIQQRSNSL+PYELLEI+HAKAEV
Sbjct: 181 RTIQQRSNSLLPYELLEIVHAKAEV 200

The following BLAST results are available for this feature:

BLAST of Cucsat.G10024 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H0X6	2.3e-58	60.41	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q0JNR2	8.8e-58	60.00	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q8LC76	3.0e-42	47.40	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]
Q06445	1.5e-28	63.54	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q41906	3.1e-26	48.31	Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=...	[more]

BLAST of Cucsat.G10024 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147084.1	3.99e-139	100.00	cysteine proteinase inhibitor 12 [Cucumis sativus] >KGN51606.1 hypothetical prot...	[more]
XP_008445944.1	3.83e-128	93.69	PREDICTED: cysteine proteinase inhibitor 12 [Cucumis melo] >TYK15792.1 cysteine ...	[more]
KAA0034127.1	1.56e-127	93.20	cysteine proteinase inhibitor 12 [Cucumis melo var. makuwa]	[more]
XP_038891963.1	1.94e-120	88.78	cysteine proteinase inhibitor 12 [Benincasa hispida]	[more]
XP_023548422.1	4.05e-117	87.32	cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G10024 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV53	1.93e-139	100.00	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_5G583360 PE=3 SV...	[more]
A0A5D3CW86	1.86e-128	93.69	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BEK2	1.86e-128	93.69	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1	[more]
A0A5A7SXY4	7.55e-128	93.20	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A6J1H1C9	7.97e-117	86.83	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 2..59 e-value: 0.0028 score: 26.9
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 3..58 e-value: 4.6E-12 score: 46.1
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 5..58 e-value: 4.36171E-13 score: 60.1804
None	No IPR available	GENE3D	3.10.450.10		coord: 1..66 e-value: 9.4E-26 score: 92.0
None	No IPR available	GENE3D	3.10.450.10		coord: 72..165 e-value: 1.3E-10 score: 43.3
None	No IPR available	PANTHER	PTHR11413:SF103	CYSTEINE PROTEINASE INHIBITOR 6	coord: 2..164
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 2..62
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 73..160
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 2..164
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 15..28

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G10024.T1	Cucsat.G10024.T1	mRNA
Cucsat.G10024.T3	Cucsat.G10024.T3	mRNA
Cucsat.G10024.T4	Cucsat.G10024.T4	mRNA
Cucsat.G10024.T2	Cucsat.G10024.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity