CsaV3_1G002730.1 (mRNA) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_1G002730.1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	SNAP25 homologous protein SNAP33
Location	chr1: 1719933 .. 1724747 (-)
Sequence length	927
RNA-Seq Expression	CsaV3_1G002730.1
Synteny	CsaV3_1G002730.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAACTGATATCAAACTGAATCATGAATGGTTCAACAATGGCGGGAATGAGGAAAAGGCCATTTCTTGGAAGTATGTTCGAAAAAGATCACTTTCAAATTCTTATATTTTATTGGTTTTGGTATATTAGTTTAATTTATTCAAAGGCTTCCTCTACTTTAACGCCAATTTCCATAATTGTTTTGTTTTGTCAAAAGCCTTCTTAATCCTCAAGTTGTTCCAATTCCTGCTGATTCTTTGGCCCAGGTCTTCCTTTGACTCTTTCCCTCTTTTAATTCCCTTCTTTCCTTCAAAACCCAGTTCAAGTCTTCGCTGGTTTATCAATTATACTCTTGATCGTCTTAGTTCTTTGATTCTGAAGGTACATGATCGCCCCTTTCTCTCTAATTTTATCTGGGTTTTCTATTGATTTCTTAATGCTTGTGGGTTTTATTTGAATTGAATGAGTGATGTCCCACTCGCTTTCTTGAGAAGTTTAATATTGTTTTGATTCTTATAATCTTTTTTTTTTTTCTTCTGTCAAATCCTATGCGATTTCTGAACTGATGATTTGGTTGATTCACCAATGCCTGCTTGCTTTTAGAGAAAACTGAGCTTGTGTAAAGAAAAGGGATAATTGGAGTTTTATTATTGATTTGTCTGTTGTGGGGTTTGGTATTAATGAACTGGCTATTTCAGGATTATCTCAGAGATGAATTTGGTTTTTGGAATATGTTTCATCAGGAACGGGGTTTTAGATTGCGTACTGTTTGATTAAATGACATGGTTTCTTGTGGAAATATGGTGTCCTCTTTAAGTTGTATTTGCTTGATTAACTTGATTTCATTACCAGAGATTATGAGTTTTTAGAACCCTTCTGATGTTCTTTTGCTGATTTGTGTGATCCATTCTTCTTTTTCATCAAATTCTTCACCAAGAACATTGTTTGTTTTTGCTTGCCTAATGTTTGATTAATTGTCATGCTTTCTTTTGGAAATACGATGTCCTGTTTAAGTTGTATTTGCCTGAATAACTTGATTTCATTACCACAGATTATGTGTTTTTAGAACCCTTTTGATCTTCTTATGCTGATTTGTGTAATCCATTTTTCTTTTTCTTGGAATTTTTCAACCAATTGTTTGACTCTTTGCTTGGTTCCAGGGTGGAATTTTGATCTTTATAAGCATTAGAATCATTCTCCTTGTCACGAAACGATCAGCTGAATTCAGCATTTGCATTTCCAGAAATGTCACCTTTTAGGAAATCCCCTATAAATGTTGGTAGGCACGGCTCAAGCCAACCTGCCAATTCTGCTCGTTCCAGTCCAAACCCAGTTAATTCAAATGATAATTTAGACAAGAAGCAAAAGCAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGCAGCACCAAATCCCAAGTCAGCTCTTTTCGACGATCATCTTGAGAAAGGGACTGACTCATCTTCATATTCTTCTTCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAGGAGTTGGAGAGTTATGCTGTTTATAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGATATCAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCACCAACAGGGCGAGCAAATCACTCGAACCCATGTGGTCACTGCTGATATTGACAACGATCTTAGTCGGGTATGTGGAATGAAGTGACCGTTATATAAATCATGATCAATAACATGTTCGAGAGTTTTCAAAATGATTAAAAACACTTTTTCATGTTCAATAATCATTCGATTCATGTTTAGTTTACACTTTTAAGCATGGTTTTTTCATCCTATCAAGATTGCTTTTAAACGATTGAAAATAATCCTGCTTTGACTTGACTTGACTTGCTGAGAACCAGTGGATGCTTTTCCTTTCCTTAACCTCCTTTCTTGTTTTAGGACTCATTGACCTCTAACCTTGATTTCATCCTAAAGAACTTAGACATCACATAGAGGGTTATTTGGCTCATGGTTTTTGGTAGCCTAAGTTAAAGAAGCCTAAAGGTTTGCAGATTGTGGGTTCGTAGTTTGTCAAAAGTATGTATATGCTGTAAAGTTTGAGTTGTATGAAATTTACCAAGCCATGAATAAAGAAACCTATGTACTAACCGGTAAACCAAACATTCCAATTTGAGTTACACAAGTGTAACCGGTAAACCAAACTTTCCAATTTGAGTTACACAAGTCTAACCGGTAAACCAAACATTACCATCTTCATCTATGGATGCATTTTGTAAAGATGTTGAAATGTTTTTGGGCATTGCCATCAATATTTGTGATGTTGATTTCAGGGTGAGAAACTTCTAGGAAGTTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGTAATATTTTTCAAATTTTGGCAGTATAATTAAGAAAAACAGAACTACCTCTTATAACTTCTTTTTCTTTGTTTTATGTCATAGTTTCTATGCATTTAAAAACAAATAAATAAGTAAAACTATACTATTATTGACGTTTATAAAAGATGAAAAAAAAAAAGAAATGGAAACACCTATGAAACACTTATGTTTGAATCAAAAAAGAAAAAAACATAAACAACTACCAAACGATCCATGAGTAACCTAACCACGATCTTAAATTATCCAAACTAAATTGAAGTCCTAATCTATAGAACAAGAAGCAACCTTTCCCACTAATCAGATGCATTAAGTTGATGGCCAATTAGTTTATCAAAAAATAACTATAGTATTTGATAAACAAACTTGATTTGGTTGTAGGGGATCATGCTGTTATAAGCAAAGGCAACCATTTGGAGCAAAGGGAGAAATTGGGGTTAAGTCGAGCACCAAACCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACAAACTCACTTCAGAAAGTTGAGGTTTGTAGTTCTTTTCTGTTTGTTAAATACAACCATTATGAGTAGGAGTTGAGTCTCCAACCTCAGGGCAAAATAGAAGCATTTTTGCAATAATTTAACAAGAAATTTGTTTCTAATCAGGTGGAAAAGGTGAAACAAGACGATGCTCTATCAGATTTGAGCAACATATTGGGAGAACTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAAGTACGGTCTTATAGTCTTAGAAAATATGTTTACCAGAGTCGTTAATTTCTTGTTTTTGCGTAGAAGAAGAACAAATAAGTAATGAGGACGTTATGAAATAAGTTCAAAAGCTAATTTTCTTATTTGATTCTTTTATTTTTGTTTAACTTAGTGTGAATTAATCCTACTATTTCAGGCAAAATAAAGCTCTGGATCACTTGTTTGATGATGCTAACGAGGTCACAATTCGTGTCGATAATGCTAACCAGCGTGCTCGCCACCTGCTTAGAAAGTAGAACGCTTTGCAAGGTCTCGTCTCATTTTTACCATCAACCAATATGATGGTAATATGCTTGACTGGAATGGCTTTCCTTTTGATTTACAGTCTCTTCCTGTCTGTCTTACTTTCCTCCAAAAGCAAAAACATTTACTTTTTTTCCCCTCAAAACAGTTGTGAAATAGTTTGGTTCACTATGTAGTTTGTAGACAAATTACATGTTTTTTCTTCTACGATTCTCTTATGGTTTTTAATGGATTCCAAAATGGAAAAAAATACATTTGGAATCCCAATCTCTCTGTACTTTTACTTTGGTTCATTTTGATCAAATATGTTCATTTTGGACTACTTTGATACTCTTATAAAGTGATCATCTTAATCTCCTCTCATTTTCACTTTATTTCATTTAAGGTGATCGAAATGGTTAACCAAAACGAAATGAAATTAATATTATAATCATCAAAATGAAAGTATAAGTAAAATTTTGATAATATAATGATTAAAACGAATCGGAAAGAAAAACAAGCTATCGAATTAATCAAGATTCATAGAACAAGTTTTCAAGACTAAAATCTGTTGTTCCAATGGATTTTATCCATACAAAAAAAAAAAAAAAAATTGGAAAGGAGATCATATCAACAAATCAAAACGAATGAGAAGATCAGTGTAATCATGTTTTCATCATCACCAAATATTTCATATTTCATCCCACAGTCAAGGATTAATTACTCAAACGATTTATTAAATTCTTAGTAGAAAAGGTATGATCCGTGAAGCAGAAATAAACCCTTACTCACGAAATCTATCACAATAGAATTAGAATTACAATAAATTAAGGAAAAAGAGCTTCCGTATTCAGTTTATGCGATGAGCCTCCATGATGCTCTTTACCTTCGGCCCATAGAGGGAGTAAACGGCGGACTGTCTTTTAGCAACTTCAAGCTGAGCCTTGCCATCGGCATAGGCGGAAGAAATGGATGGAGGATCGGAAAGGTTTCGGTTATGGCGGAAGCCATCGACCGCGCGTCGTTTGGCGTACTCTCTGATGTTGTAATCGGAGAAGTGACGGGCTGTACGGATCAGAGAGCGAAGAAGCGAAAGGGCCTCCGATCTCGTTGGGGACGCCATTTTCGAAGAAATCGATAGAATAGTTTAGTGAAAGAAAGTTTAGGGATTTGACGAAGACTTTCTTTGGCATCACAACAATAACAATAATAAATTAATAGAAAACAATATATTGTCGATTATTATACTTACGAAGGCTAAATTAAATCATATGAAATAAAATTTAATTTTGTTCTATTATATAAACTAAATAGTATTTAACTTTAATTAAAAATTTT

mRNA sequence

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGGTAGGCACGGCTCAAGCCAACCTGCCAATTCTGCTCGTTCCAGTCCAAACCCAGTTAATTCAAATGATAATTTAGACAAGAAGCAAAAGCAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGCAGCACCAAATCCCAAGTCAGCTCTTTTCGACGATCATCTTGAGAAAGGGACTGACTCATCTTCATATTCTTCTTCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAGGAGTTGGAGAGTTATGCTGTTTATAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGATATCAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCACCAACAGGGCGAGCAAATCACTCGAACCCATGTGGTCACTGCTGATATTGACAACGATCTTAGTCGGGGTGAGAAACTTCTAGGAAGTTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCATGCTGTTATAAGCAAAGGCAACCATTTGGAGCAAAGGGAGAAATTGGGGTTAAGTCGAGCACCAAACCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACAAACTCACTTCAGAAAGTTGAGGTGGAAAAGGTGAAACAAGACGATGCTCTATCAGATTTGAGCAACATATTGGGAGAACTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAAGCAAAATAAAGCTCTGGATCACTTGTTTGATGATGCTAACGAGGTCACAATTCGTGTCGATAATGCTAACCAGCGTGCTCGCCACCTGCTTAGAAAGTAG

Coding sequence (CDS)

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGGTAGGCACGGCTCAAGCCAACCTGCCAATTCTGCTCGTTCCAGTCCAAACCCAGTTAATTCAAATGATAATTTAGACAAGAAGCAAAAGCAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGCAGCACCAAATCCCAAGTCAGCTCTTTTCGACGATCATCTTGAGAAAGGGACTGACTCATCTTCATATTCTTCTTCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAGAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAAACAGTGGAGGAGTTGGAGAGTTATGCTGTTTATAAGGCTGAGGAGACAACTAACTCAGTCAACAACTGCCTGAAGATTGCTGAGGATATCAGAGAAGATGCAACCAAAACCTTAGTTGCCTTGCACCAACAGGGCGAGCAAATCACTCGAACCCATGTGGTCACTGCTGATATTGACAACGATCTTAGTCGGGGTGAGAAACTTCTAGGAAGTTTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGGGATCATGCTGTTATAAGCAAAGGCAACCATTTGGAGCAAAGGGAGAAATTGGGGTTAAGTCGAGCACCAAACCCACGGTCCTCACGTGCCCCACATCCTGAGCCAACAAACTCACTTCAGAAAGTTGAGGTGGAAAAGGTGAAACAAGACGATGCTCTATCAGATTTGAGCAACATATTGGGAGAACTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAAGCAAAATAAAGCTCTGGATCACTTGTTTGATGATGCTAACGAGGTCACAATTCGTGTCGATAATGCTAACCAGCGTGCTCGCCACCTGCTTAGAAAGTAG

Protein sequence

MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKSALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEETTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQRARHLLRK*

Homology

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BLAST of CsaV3_1G002730.1 vs. NCBI nr
Match: XP_004138184.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical protein Csa_013922 [Cucumis sativus])

HSP 1 Score: 592.0 bits (1525), Expect = 2.9e-165
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60
           MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60

Query: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120
           ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET
Sbjct: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120

Query: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180
           TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300
           KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ
Sbjct: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 309
           RARHLLRK
Sbjct: 301 RARHLLRK 308

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BLAST of CsaV3_1G002730.1 vs. NCBI nr
Match: XP_008453247.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.1 putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa])

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-156
Identity = 295/308 (95.78%), Postives = 302/308 (98.05%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60
           MSPFRKSPINV RH S+QPAN ARSSPN VNSNDNLDK+QKQSSRITSSHQ TAAPNPKS
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQKQSSRITSSHQETAAPNPKS 60

Query: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120
           ALFDDHLEKGTD SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEET
Sbjct: 61  ALFDDHLEKGTD-SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEET 120

Query: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180
           TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGIF
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300
           KVEVEKVKQ+DALSDLSNILGELKEMAVDMGSELDKQN+ALD+LFDDANEVTIRVDNANQ
Sbjct: 241 KVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 309
           RARHLLRK
Sbjct: 301 RARHLLRK 307

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BLAST of CsaV3_1G002730.1 vs. NCBI nr
Match: XP_038879751.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879753.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 547.0 bits (1408), Expect = 1.1e-151
Identity = 286/309 (92.56%), Postives = 298/309 (96.44%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60
           MSPFRKSPINV RHGSSQP NSAR SPNPV+SNDNLDKKQK +SSRITSSHQAT APNP 
Sbjct: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60

Query: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120
           S LFDDHLE+ T+S+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300
           +KVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALDHLFDDA+EVTIRVDNAN
Sbjct: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300

Query: 301 QRARHLLRK 309
           QRARHLLRK
Sbjct: 301 QRARHLLRK 309

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BLAST of CsaV3_1G002730.1 vs. NCBI nr
Match: XP_022921394.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921397.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 511.1 bits (1315), Expect = 6.5e-141
Identity = 266/309 (86.08%), Postives = 288/309 (93.20%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60
           MS FRKSPI   RHGSSQPAN ARS+  P++SNDNLDK+QK +SSRITSSHQAT APNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120
           S LFDDHLEK TDSSS+    SSYSWSSRNRYKNDFRDSGG+++QTVEELE+YA YKAEE
Sbjct: 61  SNLFDDHLEKETDSSSH----SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300
           QKVEVEKVKQ+DALSDLSNILGELK MAVDMG+ELD+QNKALD+LFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

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BLAST of CsaV3_1G002730.1 vs. NCBI nr
Match: XP_023516514.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516515.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516516.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.0 bits (1312), Expect = 1.4e-140
Identity = 265/309 (85.76%), Postives = 287/309 (92.88%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60
           MS FRKSPI   RHGSSQPAN ARS   P++SNDNLDK+QK +SSRITSSHQAT APNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSPSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120
           S LFDDHLEK TDSSS+    SSYSWSSRNRYKNDFRDSGG+++QTVEELE+YA YKAEE
Sbjct: 61  SNLFDDHLEKETDSSSH----SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGP+IFEGD AVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPIIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300
           QKVEVEKVKQ+DALSDLSNILGELK MAVDMG+ELD+QNKALD+LFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

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BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.1e-82
Identity = 180/311 (57.88%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQ--KQSSRITSSHQATAAPNP 60
           M   RKSP N+ +H S    +   S PNP +S+D  D K     S R TS        NP
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNP 60

Query: 61  KSALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAE 120
                 + ++KG  SSS  S  S    +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAE
Sbjct: 61  FGG---ERVQKGDSSSSKQSLFS----NSKYQYKNNFRDSGGIENQSVQELEGYAVYKAE 120

Query: 121 ETTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGG 180
           ETT SV  CLK+AEDIR DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG
Sbjct: 121 ETTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGG 180

Query: 181 IFSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTN 240
           +FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  +
Sbjct: 181 MFSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESAD 240

Query: 241 SLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDN 300
           + Q+VE+EK KQDD LSDLS+ILGELK MAVDMGSE++KQNK LDHL DD +E+  RV  
Sbjct: 241 AYQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQ 300

Query: 301 ANQRARHLLRK 309
           +NQR R LL K
Sbjct: 301 SNQRGRRLLGK 300

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-73
Identity = 147/220 (66.82%), Postives = 168/220 (76.36%), Query Frame = 0


Query: 89  NRYKNDFRDSGGIENQTVEELESYAVYKAEETTNSVNNCLKIAEDIREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAEDIR D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDHAVISKG 208
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D       
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQDD LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDKQNKALDHLFDDANEVTIRVDNANQRARHLLRK 309
           DMGSE+DKQNKALDHL DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 132/246 (53.66%), Postives = 171/246 (69.51%), Query Frame = 0


Query: 63  FDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEETTN 122
           FDD  E     S               R+ +  + SGG ENQTV+ELESYAVY +EETT 
Sbjct: 11  FDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEETTK 70

Query: 123 SVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSK 182
           +V  CLK+AE+IR DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG LGG+FS+
Sbjct: 71  TVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSR 130

Query: 183 TWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKV 242
           TWKPKK R ITGPVI +GD +   K   L+ REKLGL+ +  P+S     PE  ++ QK 
Sbjct: 131 TWKPKKSRSITGPVITKGD-SPKRKVIDLKTREKLGLNPSLKPKSKTL--PEAVDAYQKT 190

Query: 243 EVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQRA 302
           ++   KQD+AL+DLS +LGELK MAVDMG+ +++Q   LDHL D+A+E+  RV  +NQRA
Sbjct: 191 QI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRA 250

Query: 303 RHLLRK 309
           R+LLRK
Sbjct: 251 RYLLRK 251

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.7e-15
Identity = 62/213 (29.11%), Postives = 101/213 (47.42%), Query Frame = 0


Query: 106 VEELESYAVYKAEETTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDND 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQ 225
           +   EK L  LG               S  +K   G    G V  +    V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     D+ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDKQNKALDHLFDDANEVTIRVDNANQRARHLL 307
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.7e-15
Identity = 62/213 (29.11%), Postives = 101/213 (47.42%), Query Frame = 0


Query: 106 VEELESYAVYKAEETTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDND 165
           +EE++  A   A+E+  S    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 166 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQ 225
           +   EK L  LG               S  +K   G    G V  +    V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 226 REKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSE 285
           RE++ +S     R         TN  ++ E+     D+ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 286 LDKQNKALDHLFDDANEVTIRVDNANQRARHLL 307
           +D QN+ +D + + A+    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL
Match: A0A0A0LRS5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G012100 PE=4 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.4e-165
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60
           MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60

Query: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120
           ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET
Sbjct: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120

Query: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180
           TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300
           KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ
Sbjct: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 309
           RARHLLRK
Sbjct: 301 RARHLLRK 308

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL
Match: A0A5A7US66 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002820 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 9.1e-157
Identity = 295/308 (95.78%), Postives = 302/308 (98.05%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60
           MSPFRKSPINV RH S+QPAN ARSSPN VNSNDNLDK+QKQSSRITSSHQ TAAPNPKS
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQKQSSRITSSHQETAAPNPKS 60

Query: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120
           ALFDDHLEKGTD SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEET
Sbjct: 61  ALFDDHLEKGTD-SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEET 120

Query: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180
           TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGIF
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300
           KVEVEKVKQ+DALSDLSNILGELKEMAVDMGSELDKQN+ALD+LFDDANEVTIRVDNANQ
Sbjct: 241 KVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 309
           RARHLLRK
Sbjct: 301 RARHLLRK 307

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL
Match: A0A1S3BVT2 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103494024 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 9.1e-157
Identity = 295/308 (95.78%), Postives = 302/308 (98.05%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQKQSSRITSSHQATAAPNPKS 60
           MSPFRKSPINV RH S+QPAN ARSSPN VNSNDNLDK+QKQSSRITSSHQ TAAPNPKS
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQKQSSRITSSHQETAAPNPKS 60

Query: 61  ALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEET 120
           ALFDDHLEKGTD SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEET
Sbjct: 61  ALFDDHLEKGTD-SSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEET 120

Query: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIF 180
           TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGIF
Sbjct: 121 TNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGIF 180

Query: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240
           SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ
Sbjct: 181 SKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQ 240

Query: 241 KVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQ 300
           KVEVEKVKQ+DALSDLSNILGELKEMAVDMGSELDKQN+ALD+LFDDANEVTIRVDNANQ
Sbjct: 241 KVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNANQ 300

Query: 301 RARHLLRK 309
           RARHLLRK
Sbjct: 301 RARHLLRK 307

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL
Match: A0A6J1E197 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111429687 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.1e-141
Identity = 266/309 (86.08%), Postives = 288/309 (93.20%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60
           MS FRKSPI   RHGSSQPAN ARS+  P++SNDNLDK+QK +SSRITSSHQAT APNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120
           S LFDDHLEK TDSSS+    SSYSWSSRNRYKNDFRDSGG+++QTVEELE+YA YKAEE
Sbjct: 61  SNLFDDHLEKETDSSSH----SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300
           QKVEVEKVKQ+DALSDLSNILGELK MAVDMG+ELD+QNKALD+LFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

[Close]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL
Match: A0A6J1JJU0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111485094 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.2e-140
Identity = 266/309 (86.08%), Postives = 287/309 (92.88%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60
           MS FRKSPI   RHGSSQPA  ARS+  P++SNDN DK+QK +SSRITSSHQAT APNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120
           S LFDDHLEK TDSSS+    SSYSWSSRNRYKNDFRDSGG+++QTVEELE+YA YKAEE
Sbjct: 61  SNLFDDHLEKETDSSSH----SSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQRE+LGLSRAPNPRSSRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300
           QKVEVEKVKQ+DALSDLSNILGELK MAVDMG+ELD+QNKALDHLFDDA+E+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 309
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

[Close]

BLAST of CsaV3_1G002730.1 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 305.4 bits (781), Expect = 5.0e-83
Identity = 180/311 (57.88%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQ--KQSSRITSSHQATAAPNP 60
           M   RKSP N+ +H S    +   S PNP +S+D  D K     S R TS        NP
Sbjct: 1   MFGLRKSPANLPKHNS---VDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNP 60

Query: 61  KSALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAE 120
                 + ++KG  SSS  S  S    +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAE
Sbjct: 61  FGG---ERVQKGDSSSSKQSLFS----NSKYQYKNNFRDSGGIENQSVQELEGYAVYKAE 120

Query: 121 ETTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGG 180
           ETT SV  CLK+AEDIR DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG
Sbjct: 121 ETTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGG 180

Query: 181 IFSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRS-SRAPHPEPTN 240
           +FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S +R P PE  +
Sbjct: 181 MFSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESAD 240

Query: 241 SLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDN 300
           + Q+VE+EK KQDD LSDLS+ILGELK MAVDMGSE++KQNK LDHL DD +E+  RV  
Sbjct: 241 AYQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQ 300

Query: 301 ANQRARHLLRK 309
           +NQR R LL K
Sbjct: 301 SNQRGRRLLGK 300

[Close]

BLAST of CsaV3_1G002730.1 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-74
Identity = 147/220 (66.82%), Postives = 168/220 (76.36%), Query Frame = 0


Query: 89  NRYKNDFRDSGGIENQTVEELESYAVYKAEETTNSVNNCLKIAEDIREDATKTLVALHQQ 148
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAEDIR D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 149 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDHAVISKG 208
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D       
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 209 NHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAV 268
           NH E+REKLGL  A    SS+    +PTN+LQKVE EK KQDD LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 269 DMGSELDKQNKALDHLFDDANEVTIRVDNANQRARHLLRK 309
           DMGSE+DKQNKALDHL DD +E+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

[Close]

BLAST of CsaV3_1G002730.1 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 132/246 (53.66%), Postives = 171/246 (69.51%), Query Frame = 0


Query: 63  FDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEETTN 122
           FDD  E     S               R+ +  + SGG ENQTV+ELESYAVY +EETT 
Sbjct: 11  FDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEETTK 70

Query: 123 SVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSK 182
           +V  CLK+AE+IR DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG LGG+FS+
Sbjct: 71  TVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVFSR 130

Query: 183 TWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSLQKV 242
           TWKPKK R ITGPVI +GD +   K   L+ REKLGL+ +  P+S     PE  ++ QK 
Sbjct: 131 TWKPKKSRSITGPVITKGD-SPKRKVIDLKTREKLGLNPSLKPKSKTL--PEAVDAYQKT 190

Query: 243 EVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNANQRA 302
           ++   KQD+AL+DLS +LGELK MAVDMG+ +++Q   LDHL D+A+E+  RV  +NQRA
Sbjct: 191 QI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQRA 250

Query: 303 RHLLRK 309
           R+LLRK
Sbjct: 251 RYLLRK 251

[Close]

BLAST of CsaV3_1G002730.1 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 42.4 bits (98), Expect = 7.9e-04
Identity = 24/70 (34.29%), Postives = 36/70 (51.43%), Query Frame = 0


Query: 234 EPTNSLQKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTI 293
           E +   Q+ E+ K+KQ+  L  +S  L  LK MA DM  ELD+Q   +D +    +  T 
Sbjct: 163 ESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATS 222

Query: 294 RVDNANQRAR 304
            + N N R +
Sbjct: 223 DLKNTNVRLK 232

The following BLAST results are available for this feature:

BLAST of CsaV3_1G002730.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138184.1	2.9e-165	100.00	SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical prot...	[more]
XP_008453247.1	1.9e-156	95.78	PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978....	[more]
XP_038879751.1	1.1e-151	92.56	SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25...	[more]
XP_022921394.1	6.5e-141	86.08	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 p...	[more]
XP_023516514.1	1.4e-140	85.76	putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]

BLAST of CsaV3_1G002730.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7P9	7.1e-82	57.88	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9LMG8	1.6e-73	66.82	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9SD96	1.5e-60	53.66	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
Q17QQ3	9.7e-15	29.11	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]
P60878	9.7e-15	29.11	Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1	[more]

BLAST of CsaV3_1G002730.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRS5	1.4e-165	100.00	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A5A7US66	9.1e-157	95.78	Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BVT2	9.1e-157	95.78	putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10349402...	[more]
A0A6J1E197	3.1e-141	86.08	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JJU0	1.2e-140	86.08	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CsaV3_1G002730.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61210.1	5.0e-83	57.88	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT1G13890.1	1.1e-74	66.82	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G07880.1	1.1e-61	53.66	synaptosomal-associated protein SNAP25-like 29	[more]
AT3G09740.1	7.9e-04	34.29	syntaxin of plants 71	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 288..308
None	No IPR available	GENE3D	1.20.5.110		coord: 100..177 e-value: 2.5E-20 score: 74.2
None	No IPR available	GENE3D	1.20.5.110		coord: 228..308 e-value: 9.5E-21 score: 75.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..37
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 209..240
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..99
None	No IPR available	PANTHER	PTHR19305:SF30	TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATED	coord: 23..308
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 23..308
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 248..304 e-value: 4.06987E-13 score: 61.0352
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 243..306
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 104..174
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 109..176 e-value: 5.2 score: 7.5 coord: 238..305 e-value: 7.7E-8 score: 42.0
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 248..305 score: 14.650169
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 112..175 e-value: 1.33749E-20 score: 81.8507

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsaV3_1G002730	CsaV3_1G002730	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsaV3_1G002730.1.exon7	CsaV3_1G002730.1.exon7	exon
CsaV3_1G002730.1.exon6	CsaV3_1G002730.1.exon6	exon
CsaV3_1G002730.1.exon5	CsaV3_1G002730.1.exon5	exon
CsaV3_1G002730.1.exon4	CsaV3_1G002730.1.exon4	exon
CsaV3_1G002730.1.exon3	CsaV3_1G002730.1.exon3	exon
CsaV3_1G002730.1.exon2	CsaV3_1G002730.1.exon2	exon
CsaV3_1G002730.1.exon1	CsaV3_1G002730.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsaV3_1G002730.1.cds7	CsaV3_1G002730.1.cds7	CDS
CsaV3_1G002730.1.cds6	CsaV3_1G002730.1.cds6	CDS
CsaV3_1G002730.1.cds5	CsaV3_1G002730.1.cds5	CDS
CsaV3_1G002730.1.cds4	CsaV3_1G002730.1.cds4	CDS
CsaV3_1G002730.1.cds3	CsaV3_1G002730.1.cds3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsaV3_1G002730.1	CsaV3_1G002730.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity