Cp4.1LG10g04960.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g04960.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionserine/threonine-protein kinase HT1-like
LocationCp4.1LG10: 1119287 .. 1122381 (+)
Sequence length2432
RNA-Seq ExpressionCp4.1LG10g04960.1
SyntenyCp4.1LG10g04960.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTTACGACTTTTCCGATGTGGTTCCCACCACGCACCGTCACATCTCTCCTTCAGAAAATATCCCCTTTTCTATGGAATCTTATTTACTACCGCCCTCCAATAGTCGTTTGTTCTATGATGACAACAGATCATCGCGAAATTGAATCCCCCTTCGCCTGACAGATGGGTTTCCATGTTTTTGATTGTCTCCTAGATCATTCGACCGATTTCTTCAGTGAATGGGATGCGGAGTGAAATGAAAGGTTGGCCGCATGCTTGATTGAGGTGAGGTCTCCATTGTTCCTGGGTTTTTCCTTTTACTGCTTGTTTTTTAGGTCTCTGGAATTATTTTTTTCTCTTTTGTCGGGTTTCTGGTTAATGGGTTTGTTTGAATAGTAAAAGGGGAAGGGGATAAATTCTTTGGCCTACGACTTTTCTTGTGGTTTTTGGTTATTTTGGGGGGATTTTTCTATTTGGGTGCAATTGATCGTTTCTATTATTATTATTATTATTATTATACATAATTTGGATTTATTTATTTGTGGATCTTTGTGTGGGATTTGCCAGACTTTTACTCAAGGTGGAGTTTGACTTTGAAATCGAATTAAAAAGATGGGGCTCAAGGAGGAAATGAGATTGGAGTTAACCAGGCAGAGTGTTGGTCTGTGGCTTGCGAGCCATCTCTGTATCTGGTAGTTGGTTTTTGCTTTAAGCGATTGTTTTGCTGGTGGGAGGAACAGGATCTGGGCGATCCAAATTCTCGATTATTTATTGTGGGTCTTTGTGGGTCTCCATGGTTCTTTAGTTTGATGTAACGAACAATGTTTCTTCGTTAGTCTTGTTTGATGGGTTTGAAGAAATCGGGCGTTTGGAGCCATCAAATTAGTCCTCCTCCTGTGCAGGAGTGGGAGGAAATTAAGAACGTCTTTTGTTACAAGATCTTTTAGGGTTTTATTTCATCGAGGAATTGCAGGAATCGAGAGGGGGAAGAATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACCAAGTTTTCGCACACAGTTTATCATCGATGGGACTCATTAAGATTGAACCCTGTTCCTTTCATCGTCGAACCACCACCTCGGAATTACGGCTTGAAATCGAGGCCTCAATCAGCTTCATCCGCCCAGAAACCAAATCCTGATATCTCAAAGATTCAGCGAAATTTCATAGCCAACAAACAGCGGTCTCTGTCTCCTCTTCCCGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGCCAGTAAGAGATTCTCGACTCCAACGCCTCGACTTAGAGAACGAACTAGAGAATTCAAGAACAAGCTATTCAATAAGGACCCGCAAGATTCAAAATCGTCCACTTCCAAGACATCGTCGAATACCAGTCCCCTGAAACTCCTAGCTTCGTCCAAAGGAGGTGAAAAGTCAAAGTTCCGGAAGGACTCTTCCTGGACCAAATATTTCGATTCCGGTGGCGGAAAAGTCACAGCGGTCGAAACCGCTGACGACTGGAGCGTTGATCTATCCAAGCTGTTTGTTGGGTTAAGGTTCGCTCATGGAGCTCACAGTCGTCTTTATCATGGAAAATACAACGATGAACCTGTTGCTGTTAAACTCATTAGAGTGCCTGAGGACGACGATAGTGGAGCGCTTGCTTCTCGATTGGAGAAGCAATTCACCAGAGAAGTAACCCTTTTATCTCGTCTCCACCATCCTAATGTTTTAAAGGTAATTCAATTGTTGGGTTATAAACGATATTATGAACTGGGCTGTTCTTGGTTTTCTTGATGTTTGTTTACTTGTTGTTGCAGTTTGTAGCTGCGTGTAGGAACCCGCCAGTTTACTGTGTCATTACTGAATATTTGTCTCAAGGATCATTAAGGGCGTACTTGCTCAAACTCGAGCGCCAATCTCTCCCTTTGCAGAAACTAATTGAGTTCTCTTTAGATGTGGCTCGTGGCATGGAATACATTCATTCCCAAGGCATAATCCATCGGGATCTCAAGCCTGAGAATGTTCTTATTGATGAGGACTTCCACCTGAAAATTGCAGATTTTGGGATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCCGATGATCCCGGAACGTATCGATGGATGGCACCGGAGATGATCAAACATAAACCCTATGGTCGAAAAGTTGATGTATACAGCTTTGGGCTCATGTTGTGGGAAATGGTGTCTGGTGCAATTCCTTACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTCAATAAGGTCAGTTTTATTCATCACGTGTTCCTTGACTAAGATTCTTCTTTAGGTTTATCATGTGAGCCATGGTGTTTGATTGATACCTATTCTATGTTTGCTTCTGGAAGTTCACATGTTACATGTTTATGTAGTGAGTGCCACTGTTCCTTGCCCACGAATATCATCGATGCTCGATTTCGAGCGCTGCTTCGAAAATGTTTTAACTAAAAAAATGGCTAAGAAACAAAACTATTATGTTATTTGTTTGTATATTATGTTCTTTCAAGAAAACTAAATGAAGTTTGTGCATTGGGGTTTCCAGAATCTGAGGCCTGTTATCTCGAGCGACTGCCCGTTGGCAATGCGAGCTTTGATCGAGCAATGTTGGTCTGTGCAACCAGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAAGCTTCGCTTGCCCGTGATGGGACACTGAGTTTAACGGGGAACCCGCGGTCGAGTTTCCAAGACCATAAGAAAGGGCTGCTCCATTGGATTCAAAAGCTTGGCCCTGGCCCCCTGCATCCTGAAACCTCATCCTCCTCGCCCGTGCCGAAACCGAAATTCTCGTAACTCCATTGCTCTCCATGTTATGTCCCAACTTTCTTAGTTACCTAGCCAAGTTTTTGTTTTTGTAATTAAAAACTAAACGCTGTTCATTTCCCATTTTTTGGCTCTTGTTTATGAAGAACTTGTGTATCTTTAGTTTCATGTTCGTGCCTGGTTTCGATTCTAGCAAAATAATCAACTGGCGAGCATATCTCATAAGGTGTCAAACCATATCGAAATAATAATCACGACAGATTAAAAAAGTAAC

mRNA sequence

TATTTTACGACTTTTCCGATGTGGTTCCCACCACGCACCGTCACATCTCTCCTTCAGAAAATATCCCCTTTTCTATGGAATCTTATTTACTACCGCCCTCCAATAGTCGTTTGTTCTATGATGACAACAGATCATCGCGAAATTGAATCCCCCTTCGCCTGACAGATGGGTTTCCATGTTTTTGATTGTCTCCTAGATCATTCGACCGATTTCTTCAGTGAATGGGATGCGGAGTGAAATGAAAGGTTGGCCGCATGCTTGATTGAGACTTTTACTCAAGGTGGAGTTTGACTTTGAAATCGAATTAAAAAGATGGGGCTCAAGGAGGAAATGAGATTGGAGTTAACCAGGCAGAGTGTTGGTCTGTGGCTTGCGAGCCATCTCTGTATCTGGTAGTTGGTTTTTGCTTTAAGCGATTGTTTTGCTGGTGGGAGGAACAGGATCTGGGCGATCCAAATTCTCGATTATTTATTGTGGGTCTTTGTGGGTCTCCATGGTTCTTTAGTTTGATGTAACGAACAATGTTTCTTCGTTAGTCTTGTTTGATGGGTTTGAAGAAATCGGGCGTTTGGAGCCATCAAATTAGTCCTCCTCCTGTGCAGGAGTGGGAGGAAATTAAGAACGTCTTTTGTTACAAGATCTTTTAGGGTTTTATTTCATCGAGGAATTGCAGGAATCGAGAGGGGGAAGAATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACCAAGTTTTCGCACACAGTTTATCATCGATGGGACTCATTAAGATTGAACCCTGTTCCTTTCATCGTCGAACCACCACCTCGGAATTACGGCTTGAAATCGAGGCCTCAATCAGCTTCATCCGCCCAGAAACCAAATCCTGATATCTCAAAGATTCAGCGAAATTTCATAGCCAACAAACAGCGGTCTCTGTCTCCTCTTCCCGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGCCAGTAAGAGATTCTCGACTCCAACGCCTCGACTTAGAGAACGAACTAGAGAATTCAAGAACAAGCTATTCAATAAGGACCCGCAAGATTCAAAATCGTCCACTTCCAAGACATCGTCGAATACCAGTCCCCTGAAACTCCTAGCTTCGTCCAAAGGAGGTGAAAAGTCAAAGTTCCGGAAGGACTCTTCCTGGACCAAATATTTCGATTCCGGTGGCGGAAAAGTCACAGCGGTCGAAACCGCTGACGACTGGAGCGTTGATCTATCCAAGCTGTTTGTTGGGTTAAGGTTCGCTCATGGAGCTCACAGTCGTCTTTATCATGGAAAATACAACGATGAACCTGTTGCTGTTAAACTCATTAGAGTGCCTGAGGACGACGATAGTGGAGCGCTTGCTTCTCGATTGGAGAAGCAATTCACCAGAGAAGTAACCCTTTTATCTCGTCTCCACCATCCTAATGTTTTAAAGTTTGTAGCTGCGTGTAGGAACCCGCCAGTTTACTGTGTCATTACTGAATATTTGTCTCAAGGATCATTAAGGGCGTACTTGCTCAAACTCGAGCGCCAATCTCTCCCTTTGCAGAAACTAATTGAGTTCTCTTTAGATGTGGCTCGTGGCATGGAATACATTCATTCCCAAGGCATAATCCATCGGGATCTCAAGCCTGAGAATGTTCTTATTGATGAGGACTTCCACCTGAAAATTGCAGATTTTGGGATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCCGATGATCCCGGAACGTATCGATGGATGGCACCGGAGATGATCAAACATAAACCCTATGGTCGAAAAGTTGATGTATACAGCTTTGGGCTCATGTTGTGGGAAATGGTGTCTGGTGCAATTCCTTACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTCAATAAGAATCTGAGGCCTGTTATCTCGAGCGACTGCCCGTTGGCAATGCGAGCTTTGATCGAGCAATGTTGGTCTGTGCAACCAGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAAGCTTCGCTTGCCCGTGATGGGACACTGAGTTTAACGGGGAACCCGCGGTCGAGTTTCCAAGACCATAAGAAAGGGCTGCTCCATTGGATTCAAAAGCTTGGCCCTGGCCCCCTGCATCCTGAAACCTCATCCTCCTCGCCCGTGCCGAAACCGAAATTCTCGTAACTCCATTGCTCTCCATGTTATGTCCCAACTTTCTTAGTTACCTAGCCAAGTTTTTGTTTTTGTAATTAAAAACTAAACGCTGTTCATTTCCCATTTTTTGGCTCTTGTTTATGAAGAACTTGTGTATCTTTAGTTTCATGTTCGTGCCTGGTTTCGATTCTAGCAAAATAATCAACTGGCGAGCATATCTCATAAGGTGTCAAACCATATCGAAATAATAATCACGACAGATTAAAAAAGTAAC

Coding sequence (CDS)

ATGGACGAAGAGGCTAATTCTTGGCTTAGGAGAACCAAGTTTTCGCACACAGTTTATCATCGATGGGACTCATTAAGATTGAACCCTGTTCCTTTCATCGTCGAACCACCACCTCGGAATTACGGCTTGAAATCGAGGCCTCAATCAGCTTCATCCGCCCAGAAACCAAATCCTGATATCTCAAAGATTCAGCGAAATTTCATAGCCAACAAACAGCGGTCTCTGTCTCCTCTTCCCGAGAGCAACCTCTCTGAAGTTTTCAAGGAAGCAAAATCCGCCAGTAAGAGATTCTCGACTCCAACGCCTCGACTTAGAGAACGAACTAGAGAATTCAAGAACAAGCTATTCAATAAGGACCCGCAAGATTCAAAATCGTCCACTTCCAAGACATCGTCGAATACCAGTCCCCTGAAACTCCTAGCTTCGTCCAAAGGAGGTGAAAAGTCAAAGTTCCGGAAGGACTCTTCCTGGACCAAATATTTCGATTCCGGTGGCGGAAAAGTCACAGCGGTCGAAACCGCTGACGACTGGAGCGTTGATCTATCCAAGCTGTTTGTTGGGTTAAGGTTCGCTCATGGAGCTCACAGTCGTCTTTATCATGGAAAATACAACGATGAACCTGTTGCTGTTAAACTCATTAGAGTGCCTGAGGACGACGATAGTGGAGCGCTTGCTTCTCGATTGGAGAAGCAATTCACCAGAGAAGTAACCCTTTTATCTCGTCTCCACCATCCTAATGTTTTAAAGTTTGTAGCTGCGTGTAGGAACCCGCCAGTTTACTGTGTCATTACTGAATATTTGTCTCAAGGATCATTAAGGGCGTACTTGCTCAAACTCGAGCGCCAATCTCTCCCTTTGCAGAAACTAATTGAGTTCTCTTTAGATGTGGCTCGTGGCATGGAATACATTCATTCCCAAGGCATAATCCATCGGGATCTCAAGCCTGAGAATGTTCTTATTGATGAGGACTTCCACCTGAAAATTGCAGATTTTGGGATAGCTTGTCCTGAGGCCTTTTTTGACCCATTAGCCGATGATCCCGGAACGTATCGATGGATGGCACCGGAGATGATCAAACATAAACCCTATGGTCGAAAAGTTGATGTATACAGCTTTGGGCTCATGTTGTGGGAAATGGTGTCTGGTGCAATTCCTTACGAGGATATGACTCCCATTCAAGCAGCCTTTGCTGTTGTCAATAAGAATCTGAGGCCTGTTATCTCGAGCGACTGCCCGTTGGCAATGCGAGCTTTGATCGAGCAATGTTGGTCTGTGCAACCAGACAAGAGACCTGATTTCTGGCAGATTGTGAAGGTATTAGAGCAATTCGAAGCTTCGCTTGCCCGTGATGGGACACTGAGTTTAACGGGGAACCCGCGGTCGAGTTTCCAAGACCATAAGAAAGGGCTGCTCCATTGGATTCAAAAGCTTGGCCCTGGCCCCCTGCATCCTGAAACCTCATCCTCCTCGCCCGTGCCGAAACCGAAATTCTCGTAA

Protein sequence

MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDISKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDPQDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPGPLHPETSSSSPVPKPKFS
Homology
BLAST of Cp4.1LG10g04960.1 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.8e-90
Identity = 171/380 (45.00%), Postives = 246/380 (64.74%), Query Frame = 0

Query: 115 LFNKDPQDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETA 174
           L +K P +        + ++S     A ++  EK +F    SW+   +S   +       
Sbjct: 15  LRSKLPLEPSLPNLPCNPSSSKTNRYAEAETMEKKRFDSMESWSMILESENVETWEASKG 74

Query: 175 --DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQF 234
             ++W+ DLS+LF+G +FA GAHSR+Y G Y    VAVK++R+P   +     ++LE+QF
Sbjct: 75  EREEWTADLSQLFIGNKFASGAHSRIYRGIYKQRAVAVKMVRIPTHKEE--TRAKLEQQF 134

Query: 235 TREVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEF 294
             EV LLSRL HPN+++F+AAC+ PPVYC+ITEY+SQG+LR YL K E  SL ++ ++  
Sbjct: 135 KSEVALLSRLFHPNIVQFIAACKKPPVYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRL 194

Query: 295 SLDVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRW 354
           +LD++RGMEY+HSQG+IHRDLK  N+L++++  +K+ADFG +C E        + GTYRW
Sbjct: 195 ALDISRGMEYLHSQGVIHRDLKSNNLLLNDEMRVKVADFGTSCLETQCREAKGNMGTYRW 254

Query: 355 MAPEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCP 414
           MAPEMIK KPY RKVDVYSFG++LWE+ +  +P++ MTP+QAAFAV  KN RP + + C 
Sbjct: 255 MAPEMIKEKPYTRKVDVYSFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASCQ 314

Query: 415 LAMRALIEQCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLH 474
            A+  LI++CWS  P KRPDF  IV VLE+++    ++G L LT +  +S    KK +L 
Sbjct: 315 PALAHLIKRCWSENPSKRPDFSNIVAVLEKYD-ECVKEG-LPLTSH--ASLTKTKKAILD 374

Query: 475 WIQKLGPGPLHPETSSSSPV 493
            ++        P +SSS PV
Sbjct: 375 HLKGCVTSISSPFSSSSVPV 388

BLAST of Cp4.1LG10g04960.1 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.5e-78
Identity = 128/273 (46.89%), Postives = 196/273 (71.79%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           D+W++DL KL +G  FA GA  +LY G YN E VA+K++  PE  +S   A  +E+QF +
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPE--NSPEKAQFMEQQFQQ 180

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV++L+ L HPN+++F+ ACR P V+C++TEY   GS+R +L + + +++PL+  ++ +L
Sbjct: 181 EVSMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQAL 240

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           DVARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  E   + +  + GTYRWMA
Sbjct: 241 DVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMA 300

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PEMI+H+ Y +KVDVYSFG++LWE+++G +P+++MT +QAAFAVVN+ +RP + +DC   
Sbjct: 301 PEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPV 360

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQFEASL 448
           +  ++ +CW   P+ RP F ++VK+LE  E  +
Sbjct: 361 LSDIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of Cp4.1LG10g04960.1 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 114/277 (41.16%), Postives = 178/277 (64.26%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           D W ++L  L  G + A G++  LY G Y  + VA+K+++ PE      L S LEK+F +
Sbjct: 281 DVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLK-PE-----RLDSDLEKEFAQ 340

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV ++ ++ H NV++F+ AC  PP  C++TE++  GS+  YL K ++    L  L + ++
Sbjct: 341 EVFIMRKVRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHK-QKGVFKLPTLFKVAI 400

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           D+ +GM Y+H   IIHRDLK  N+L+DE+  +K+ADFG+A  +A    +  + GTYRWMA
Sbjct: 401 DICKGMSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAETGTYRWMA 460

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PE+I+HKPY  K DV+S+G++LWE+++G +PYE MTP+QAA  VV K LRP I  +    
Sbjct: 461 PEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPK 520

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQFEASLARDG 452
           +  L+E+ W     +RPDF +I++ L++    +  +G
Sbjct: 521 LAELLERLWEHDSTQRPDFSEIIEQLQEIAKEVGEEG 550

BLAST of Cp4.1LG10g04960.1 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 2.9e-61
Identity = 105/268 (39.18%), Postives = 179/268 (66.79%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           D+W +D+++L +  + A G++  L+ G Y  + VA+K ++         + + + ++F++
Sbjct: 277 DEWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLK------PDRVNNEMLREFSQ 336

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV ++ ++ H NV++F+ AC   P  C++TE++++GS+  +L K ++ +  LQ L++ +L
Sbjct: 337 EVFIMRKVRHKNVVQFLGACTRSPTLCIVTEFMARGSIYDFLHK-QKCAFKLQTLLKVAL 396

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           DVA+GM Y+H   IIHRDLK  N+L+DE   +K+ADFG+A  +     +  + GTYRWMA
Sbjct: 397 DVAKGMSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESGVMTAETGTYRWMA 456

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PE+I+HKPY  K DV+S+ ++LWE+++G IPY  +TP+QAA  VV K LRP I       
Sbjct: 457 PEVIEHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPK 516

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQ 443
           ++ L+E+CW   P++RP F +I+++L+Q
Sbjct: 517 VKGLLERCWHQDPEQRPLFEEIIEMLQQ 537

BLAST of Cp4.1LG10g04960.1 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.5e-60
Identity = 104/276 (37.68%), Postives = 179/276 (64.86%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           D+W +D+ +L +  + A G++  L+ G Y  + VA+K+++ PE      + + + ++F++
Sbjct: 283 DEWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILK-PE-----RVNAEMLREFSQ 342

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV ++ ++ H NV++F+ AC   P  C++TE++++GS+  +L K  +    +Q L++ +L
Sbjct: 343 EVYIMRKVRHKNVVQFIGACTRSPNLCIVTEFMTRGSIYDFLHK-HKGVFKIQSLLKVAL 402

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           DV++GM Y+H   IIHRDLK  N+L+DE   +K+ADFG+A  +     +  + GTYRWMA
Sbjct: 403 DVSKGMNYLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESGVMTAETGTYRWMA 462

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PE+I+HKPY  + DV+S+ ++LWE+++G +PY  +TP+QAA  VV K LRP I  +    
Sbjct: 463 PEVIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPK 522

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQFEASLARD 451
           +  L+E+CW   P  RP+F +I+++L Q    +  D
Sbjct: 523 LTELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDD 551

BLAST of Cp4.1LG10g04960.1 vs. NCBI nr
Match: XP_023544272.1 (serine/threonine-protein kinase HT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1002 bits (2590), Expect = 0.0
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. NCBI nr
Match: KAG6604450.1 (Serine/threonine/tyrosine-protein kinase HT1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034595.1 Serine/threonine-protein kinase HT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1000 bits (2586), Expect = 0.0
Identity = 496/498 (99.60%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPF+VEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFVVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSSTSKTSSNTSPLKLLAS+KGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSTSKTSSNTSPLKLLASAKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. NCBI nr
Match: XP_022925883.1 (serine/threonine-protein kinase HT1-like [Cucurbita moschata])

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 495/498 (99.40%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPF+VEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFVVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSSTSKTSSNTSPLKLLAS+KGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSTSKTSSNTSPLKLLASAKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEAS+ARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASVARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. NCBI nr
Match: XP_022978683.1 (serine/threonine-protein kinase HT1 [Cucurbita maxima])

HSP 1 Score: 996 bits (2575), Expect = 0.0
Identity = 494/498 (99.20%), Postives = 496/498 (99.60%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLS+VFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSQVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSS SKTSSNTSPLKLLAS+KGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSNSKTSSNTSPLKLLASAKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDV RGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVGRGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. NCBI nr
Match: XP_038882155.1 (serine/threonine/tyrosine-protein kinase HT1 [Benincasa hispida] >XP_038882156.1 serine/threonine/tyrosine-protein kinase HT1 [Benincasa hispida])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 457/498 (91.77%), Postives = 477/498 (95.78%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP RN GLKSRPQSASS QKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP-RNSGLKSRPQSASSTQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQR+FI+NKQRSLSPLPESNLSEVFKEAKS SKRFSTPTPRLRERT+EFKNKLFNKDP
Sbjct: 61  SKIQRSFISNKQRSLSPLPESNLSEVFKEAKSDSKRFSTPTPRLRERTKEFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSK + SK+S NTSPLKLL+S K G+KSKFRKDSSWTKYFDSGGGKVTAVETADDW+VD
Sbjct: 121 QDSKLADSKSSLNTSPLKLLSSGKRGDKSKFRKDSSWTKYFDSGGGKVTAVETADDWTVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVK+IRVP+DD++G LASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNV+KFVAACRNPPVYCVITEYLS+GSLRAYL KLE QSLPLQKLIEF+LDVARGM
Sbjct: 241 RLHHPNVIKFVAACRNPPVYCVITEYLSRGSLRAYLHKLEHQSLPLQKLIEFALDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EY+HSQG+IHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYLHSQGVIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KP  RKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWS+QPDKRPDFWQIVKVLEQFE+SLA DGTL+L GNP SSFQDHKKGLLHWIQKLGP 
Sbjct: 421 QCWSLQPDKRPDFWQIVKVLEQFESSLAHDGTLNLLGNPLSSFQDHKKGLLHWIQKLGP- 480

Query: 481 PLHPETSSSSPVPKPKFS 498
            LHPETS S PVPKPKFS
Sbjct: 481 -LHPETSPS-PVPKPKFS 494

BLAST of Cp4.1LG10g04960.1 vs. ExPASy TrEMBL
Match: A0A6J1EGH1 (serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111433159 PE=4 SV=1)

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 495/498 (99.40%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPF+VEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFVVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSSTSKTSSNTSPLKLLAS+KGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSTSKTSSNTSPLKLLASAKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEAS+ARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASVARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. ExPASy TrEMBL
Match: A0A6J1ITY7 (serine/threonine-protein kinase HT1 OS=Cucurbita maxima OX=3661 GN=LOC111478573 PE=4 SV=1)

HSP 1 Score: 996 bits (2575), Expect = 0.0
Identity = 494/498 (99.20%), Postives = 496/498 (99.60%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQRNFIANKQRSLSPLPESNLS+VFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP
Sbjct: 61  SKIQRNFIANKQRSLSPLPESNLSQVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSS SKTSSNTSPLKLLAS+KGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD
Sbjct: 121 QDSKSSNSKTSSNTSPLKLLASAKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDV RGM
Sbjct: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVGRGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG
Sbjct: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480

Query: 481 PLHPETSSSSPVPKPKFS 498
           PLHPETSSSSPVPKPKFS
Sbjct: 481 PLHPETSSSSPVPKPKFS 498

BLAST of Cp4.1LG10g04960.1 vs. ExPASy TrEMBL
Match: A0A0A0KNC4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517390 PE=4 SV=1)

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 451/498 (90.56%), Postives = 474/498 (95.18%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSL+LNPVPFIVEPP RN GLKSRP SASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLKLNPVPFIVEPP-RNSGLKSRPPSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQR+FI+NKQRSLSPLPESNLSEVFKEAKS SKRFSTPTPRLRERT+EFKNKLFNKDP
Sbjct: 61  SKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRERTKEFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKSS SK+S NTSPLK L+S KGG+KSK +KDSSWTKYFDS  GKVTAVETADDW+VD
Sbjct: 121 QDSKSSNSKSSLNTSPLKQLSSGKGGDKSKLKKDSSWTKYFDS--GKVTAVETADDWTVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVK+IRVP+DD++G LA+RLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLAARLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RL+HPNV+KFVAACRNPPVYCVITEYLSQGSLRAYL KLE QSLPLQKLI+F+LDVARGM
Sbjct: 241 RLYHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EY+HSQG+IHRDLKPENVLIDED HLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KP  RKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWS+QPDKRPDFWQIVKVLEQFE+SLARDGTL+L GNP SSF DHKKGLLHWIQKLGP 
Sbjct: 421 QCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHDHKKGLLHWIQKLGP- 480

Query: 481 PLHPETSSSSPVPKPKFS 498
            LHPE SSS PVPKPKFS
Sbjct: 481 -LHPEVSSS-PVPKPKFS 492

BLAST of Cp4.1LG10g04960.1 vs. ExPASy TrEMBL
Match: A0A5A7UBD3 (Serine/threonine-protein kinase HT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007500 PE=4 SV=1)

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 448/498 (89.96%), Postives = 470/498 (94.38%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP RN GLKSRP SASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP-RNSGLKSRPSSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQR+FI+NKQRSLSPLPESNLSEVFKEAKS SKRFSTPTPRLR RT+EFKNKLFNKDP
Sbjct: 61  SKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRGRTKEFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKS+ SK S NTSPLK L+  KGG+KSK +KDSSWTKYFDS  GKVTAVE+ADDW+VD
Sbjct: 121 QDSKSANSKPSLNTSPLKQLSLGKGGDKSKLKKDSSWTKYFDS--GKVTAVESADDWTVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVK+IRVP+DD++G LASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNV+KFVAACRNPPVYCVITEYLSQGSLRAYL KLE QSLPLQKLI+F+LDVARGM
Sbjct: 241 RLHHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EY+HSQG+IHRDLKPENVLIDED HLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KP  RKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWS+QPDKRPDFWQIVKVLEQFE+SLARDGTL+L GNP SSF +HKKGLLHWIQKLGP 
Sbjct: 421 QCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHEHKKGLLHWIQKLGP- 480

Query: 481 PLHPETSSSSPVPKPKFS 498
            LHPETS   PVPKPKFS
Sbjct: 481 -LHPETSPL-PVPKPKFS 492

BLAST of Cp4.1LG10g04960.1 vs. ExPASy TrEMBL
Match: A0A1S3AZA8 (serine/threonine-protein kinase HT1 OS=Cucumis melo OX=3656 GN=LOC103484517 PE=4 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 449/498 (90.16%), Postives = 470/498 (94.38%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP RN GLKSRP SASSAQKPNPDI
Sbjct: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPP-RNSGLKSRPSSASSAQKPNPDI 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFNKDP 120
           SKIQR+FI+NKQRSLSPLPESNLSEVFKEAKS SKRFSTPTPRLR RT+EFKNKLFNKDP
Sbjct: 61  SKIQRSFISNKQRSLSPLPESNLSEVFKEAKSESKRFSTPTPRLRGRTKEFKNKLFNKDP 120

Query: 121 QDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSVD 180
           QDSKS+ SK S NTSPLK L+S KGG+KSK  KDSSWTKYFDS  GKVTAVE+ADDW+VD
Sbjct: 121 QDSKSANSKPSLNTSPLKQLSSGKGGDKSKL-KDSSWTKYFDS--GKVTAVESADDWTVD 180

Query: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLS 240
           LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVK+IRVP+DD++G LASRLEKQFTREVTLLS
Sbjct: 181 LSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKIIRVPDDDENGTLASRLEKQFTREVTLLS 240

Query: 241 RLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGM 300
           RLHHPNV+KFVAACRNPPVYCVITEYLSQGSLRAYL KLE QSLPLQKLI+F+LDVARGM
Sbjct: 241 RLHHPNVIKFVAACRNPPVYCVITEYLSQGSLRAYLHKLEHQSLPLQKLIKFALDVARGM 300

Query: 301 EYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360
           EY+HSQG+IHRDLKPENVLIDED HLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH
Sbjct: 301 EYLHSQGVIHRDLKPENVLIDEDMHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKH 360

Query: 361 KPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420
           KP  RKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE
Sbjct: 361 KPCSRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIE 420

Query: 421 QCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPG 480
           QCWS+QPDKRPDFWQIVKVLEQFE+SLARDGTL+L GNP SSF +HKKGLLHWIQKLGP 
Sbjct: 421 QCWSLQPDKRPDFWQIVKVLEQFESSLARDGTLNLLGNPLSSFHEHKKGLLHWIQKLGP- 480

Query: 481 PLHPETSSSSPVPKPKFS 498
            LHPETS   PVPKPKFS
Sbjct: 481 -LHPETSPL-PVPKPKFS 491

BLAST of Cp4.1LG10g04960.1 vs. TAIR 10
Match: AT5G58950.1 (Protein kinase superfamily protein )

HSP 1 Score: 591.7 bits (1524), Expect = 5.7e-169
Identity = 309/530 (58.30%), Postives = 390/530 (73.58%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQ---SASSAQKPN 60
           MDEEA SW+RR KFS TV +R +S +L  +PF++    +  GLK+ PQ   S+SSA   +
Sbjct: 1   MDEEATSWIRRAKFSQTVSYRLNSSKLASLPFMIN-QDKFSGLKAIPQRSSSSSSASSSD 60

Query: 61  PDI-----------------------SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSAS 120
           P +                       S+IQ N + +KQRS+SP P+  + +VFKEA+S  
Sbjct: 61  PKLVSSNSQTTGDTSSLEAADVYVVDSEIQTNPVTHKQRSVSPSPQMAVPDVFKEARSER 120

Query: 121 KRFSTPTPRLRERTREFKNKLFNKDPQDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKD 180
           KRFSTP PR  E  +  K KL +K+  D +S   ++ S   P++ L + +  E+ K +KD
Sbjct: 121 KRFSTPHPRRVESEKGMKPKLSHKNSFDKRSFNLRSPS--GPIRDLGTLRIQERVKSKKD 180

Query: 181 SSWTKYFDSGGGKVTAVETADDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIR 240
           + W+K FD+ G +V+AVE ++++ VD+SKLF GL+FAHG +SRLYHGKY D+ VAVKLI 
Sbjct: 181 TGWSKLFDNTGRRVSAVEASEEFRVDMSKLFFGLKFAHGLYSRLYHGKYEDKAVAVKLIT 240

Query: 241 VPEDDDSGALASRLEKQFTREVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRA 300
           VP+DDD+G L +RLEKQFT+EVTLLSRL HPNV+KFV A ++PPVYCV+T+YL +GSLR+
Sbjct: 241 VPDDDDNGCLGARLEKQFTKEVTLLSRLTHPNVIKFVGAYKDPPVYCVLTQYLPEGSLRS 300

Query: 301 YLLKLERQSLPLQKLIEFSLDVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIA 360
           +L K E +SLPL+KLIEF++D+ARGMEYIHS+ IIHRDLKPENVLIDE+FHLKIADFGIA
Sbjct: 301 FLHKPENRSLPLKKLIEFAIDIARGMEYIHSRRIIHRDLKPENVLIDEEFHLKIADFGIA 360

Query: 361 CPEAFFDPLADDPGTYRWMAPEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQA 420
           C E + D LADDPGTYRWMAPEMIK KP+GRK DVYSFGL+LWEMV+GAIPYEDM PIQA
Sbjct: 361 CEEEYCDMLADDPGTYRWMAPEMIKRKPHGRKADVYSFGLVLWEMVAGAIPYEDMNPIQA 420

Query: 421 AFAVVNKNLRPVISSDCPLAMRALIEQCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLS 480
           AFAVV+KN+RP I  DCP+AM+ALIEQCWSV PDKRP+FWQIVKVLEQF  SL R+G L+
Sbjct: 421 AFAVVHKNIRPAIPGDCPVAMKALIEQCWSVAPDKRPEFWQIVKVLEQFAISLEREGNLN 480

Query: 481 LTGNPRSSFQDHKKGLLHWIQKLGP------GPLHPETSSSSPVPKPKFS 499
           L+ +     +D +KGL HWIQKLGP      G         S +PKPKF+
Sbjct: 481 LSSS--KICKDPRKGLKHWIQKLGPVHAGGGGGSSSSGLGGSALPKPKFA 525

BLAST of Cp4.1LG10g04960.1 vs. TAIR 10
Match: AT3G46930.1 (Protein kinase superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 3.4e-129
Identity = 252/504 (50.00%), Postives = 330/504 (65.48%), Query Frame = 0

Query: 1   MDEEANSWLRRTKFSHTVYHRWDSLRLNPVPFIVEPPPRNYGLKSRPQSASSAQKPNPDI 60
           MD E  SW+RR  FSHTV +R  +  L  +PF V                          
Sbjct: 1   MDGEVTSWIRRANFSHTVCYRMITPSLESMPFTVNQ------------------------ 60

Query: 61  SKIQRNFIANKQRSLSPLPESNLSEVFKEAKSASKRFSTPTPRLRERTREFKNKLFN-KD 120
            K+QRN + NK+RS+SPLP   LS+ F EAKS  KRFSTP PR  E  +  K K  + KD
Sbjct: 61  EKMQRNPVTNKKRSVSPLPHMALSDAFIEAKSDIKRFSTPHPRRVEPEKGMKAKSSSRKD 120

Query: 121 PQDSKSSTSKTSSNTSPLKLLASSKGGEKSKFRKDSSWTKYFDSGGGKVTAVETADDWSV 180
             + KS   ++ S++ P++ L++ K  E+ K + D   +K  D  G KV++    ++  +
Sbjct: 121 SSEKKSVNLRSLSHSGPIRDLSTQKVKERGKSKIDKKSSKSVDYRGSKVSSAGVLEECLI 180

Query: 181 DLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLL 240
           D+SKL  G RFAHG +S++YHG+Y  + VA+K+I  PED D   L +RLEK+F  E TLL
Sbjct: 181 DVSKLSYGDRFAHGKYSQIYHGEYEGKAVALKIITAPEDSDDIFLGARLEKEFIVEATLL 240

Query: 241 SRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARG 300
           SRL HPNV+KFV         C+ITEY+ +GSLR+YL KLE++SLPL++LI+F LD+A+G
Sbjct: 241 SRLSHPNVVKFVGVNTG---NCIITEYVPRGSLRSYLHKLEQKSLPLEQLIDFGLDIAKG 300

Query: 301 MEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIK 360
           MEYIHS+ I+H+DLKPENVLID DFHLKIADFGIAC E + D L D+ GTYRWMAPE++K
Sbjct: 301 MEYIHSREIVHQDLKPENVLIDNDFHLKIADFGIACEEEYCDVLGDNIGTYRWMAPEVLK 360

Query: 361 HKPYGRKVDVYSFGLMLWEMVSGAIPYEDMT-PIQAAFAVVNKNLRPVISSDCPLAMRAL 420
             P+GRK DVYSFGL+LWEMV+GA+PYE+M    Q A+AV+ K +RPVI +DCP AM+ L
Sbjct: 361 RIPHGRKCDVYSFGLLLWEMVAGALPYEEMKFAEQIAYAVIYKKIRPVIPTDCPAAMKEL 420

Query: 421 IEQCWSVQPDKRPDFWQIVKVLEQFEASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLG 480
           IE+CWS Q DKRP+FWQIVKVLE F+ SL  +G L+L   P     + KK    WI   G
Sbjct: 421 IERCWSSQTDKRPEFWQIVKVLEHFKKSLTSEGKLNLL--PSQICPELKKCPKFWIHIFG 475

Query: 481 PGPLHP----ETSSSSPVPKPKFS 499
               H      +S++S +PKPKF+
Sbjct: 481 SFHHHSSGGGSSSNNSALPKPKFA 475

BLAST of Cp4.1LG10g04960.1 vs. TAIR 10
Match: AT1G62400.1 (Protein kinase superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 6.6e-93
Identity = 166/348 (47.70%), Postives = 234/348 (67.24%), Query Frame = 0

Query: 147 EKSKFRKDSSWTKYFDSGGGKVTAVETA--DDWSVDLSKLFVGLRFAHGAHSRLYHGKYN 206
           EK +F    SW+   +S   +         ++W+ DLS+LF+G +FA GAHSR+Y G Y 
Sbjct: 2   EKKRFDSMESWSMILESENVETWEASKGEREEWTADLSQLFIGNKFASGAHSRIYRGIYK 61

Query: 207 DEPVAVKLIRVPEDDDSGALASRLEKQFTREVTLLSRLHHPNVLKFVAACRNPPVYCVIT 266
              VAVK++R+P   +     ++LE+QF  EV LLSRL HPN+++F+AAC+ PPVYC+IT
Sbjct: 62  QRAVAVKMVRIPTHKEE--TRAKLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVYCIIT 121

Query: 267 EYLSQGSLRAYLLKLERQSLPLQKLIEFSLDVARGMEYIHSQGIIHRDLKPENVLIDEDF 326
           EY+SQG+LR YL K E  SL ++ ++  +LD++RGMEY+HSQG+IHRDLK  N+L++++ 
Sbjct: 122 EYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLLNDEM 181

Query: 327 HLKIADFGIACPEAFFDPLADDPGTYRWMAPEMIKHKPYGRKVDVYSFGLMLWEMVSGAI 386
            +K+ADFG +C E        + GTYRWMAPEMIK KPY RKVDVYSFG++LWE+ +  +
Sbjct: 182 RVKVADFGTSCLETQCREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTALL 241

Query: 387 PYEDMTPIQAAFAVVNKNLRPVISSDCPLAMRALIEQCWSVQPDKRPDFWQIVKVLEQFE 446
           P++ MTP+QAAFAV  KN RP + + C  A+  LI++CWS  P KRPDF  IV VLE+++
Sbjct: 242 PFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVLEKYD 301

Query: 447 ASLARDGTLSLTGNPRSSFQDHKKGLLHWIQKLGPGPLHPETSSSSPV 493
               ++G L LT +  +S    KK +L  ++        P +SSS PV
Sbjct: 302 -ECVKEG-LPLTSH--ASLTKTKKAILDHLKGCVTSISSPFSSSSVPV 343

BLAST of Cp4.1LG10g04960.1 vs. TAIR 10
Match: AT2G24360.1 (Protein kinase superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-79
Identity = 128/273 (46.89%), Postives = 196/273 (71.79%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           D+W++DL KL +G  FA GA  +LY G YN E VA+K++  PE  +S   A  +E+QF +
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPE--NSPEKAQFMEQQFQQ 180

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV++L+ L HPN+++F+ ACR P V+C++TEY   GS+R +L + + +++PL+  ++ +L
Sbjct: 181 EVSMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQAL 240

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           DVARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  E   + +  + GTYRWMA
Sbjct: 241 DVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMA 300

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PEMI+H+ Y +KVDVYSFG++LWE+++G +P+++MT +QAAFAVVN+ +RP + +DC   
Sbjct: 301 PEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPV 360

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQFEASL 448
           +  ++ +CW   P+ RP F ++VK+LE  E  +
Sbjct: 361 LSDIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of Cp4.1LG10g04960.1 vs. TAIR 10
Match: AT4G31170.1 (Protein kinase superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 2.7e-78
Identity = 128/273 (46.89%), Postives = 192/273 (70.33%), Query Frame = 0

Query: 175 DDWSVDLSKLFVGLRFAHGAHSRLYHGKYNDEPVAVKLIRVPEDDDSGALASRLEKQFTR 234
           ++W++DL KL +G  FA GA  +LY G YN E VA+KL+     D +   A  LE+QF +
Sbjct: 122 EEWTIDLRKLHMGPAFAQGAFGKLYRGTYNGEDVAIKLLE--RSDSNPEKAQALEQQFQQ 181

Query: 235 EVTLLSRLHHPNVLKFVAACRNPPVYCVITEYLSQGSLRAYLLKLERQSLPLQKLIEFSL 294
           EV++L+ L HPN+++F+ AC  P V+C++TEY   GS+R +L K + +++PL+  +  +L
Sbjct: 182 EVSMLAFLKHPNIVRFIGACIKPMVWCIVTEYAKGGSVRQFLTKRQNRAVPLKLAVMQAL 241

Query: 295 DVARGMEYIHSQGIIHRDLKPENVLIDEDFHLKIADFGIACPEAFFDPLADDPGTYRWMA 354
           DVARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  E   + +  + GTYRWMA
Sbjct: 242 DVARGMAYVHERNFIHRDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMA 301

Query: 355 PEMIKHKPYGRKVDVYSFGLMLWEMVSGAIPYEDMTPIQAAFAVVNKNLRPVISSDCPLA 414
           PEMI+H+PY +KVDVYSFG++LWE+++G +P+++MT +QAAFAVVN+ +RP + +DC   
Sbjct: 302 PEMIQHRPYTQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPADCLPV 361

Query: 415 MRALIEQCWSVQPDKRPDFWQIVKVLEQFEASL 448
           +  ++ +CW   P+ RP F +IV +LE  E  +
Sbjct: 362 LGEIMTRCWDADPEVRPCFAEIVNLLEAAETEI 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2MHE41.8e-9045.00Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZQ311.5e-7846.89Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 P... [more]
F4JTP53.1e-6341.16Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
O225582.9e-6139.18Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
Q8RWL62.5e-6037.68Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
Match NameE-valueIdentityDescription
XP_023544272.10.0100.00serine/threonine-protein kinase HT1 [Cucurbita pepo subsp. pepo][more]
KAG6604450.10.099.60Serine/threonine/tyrosine-protein kinase HT1, partial [Cucurbita argyrosperma su... [more]
XP_022925883.10.099.40serine/threonine-protein kinase HT1-like [Cucurbita moschata][more]
XP_022978683.10.099.20serine/threonine-protein kinase HT1 [Cucurbita maxima][more]
XP_038882155.10.091.77serine/threonine/tyrosine-protein kinase HT1 [Benincasa hispida] >XP_038882156.1... [more]
Match NameE-valueIdentityDescription
A0A6J1EGH10.099.40serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1ITY70.099.20serine/threonine-protein kinase HT1 OS=Cucurbita maxima OX=3661 GN=LOC111478573 ... [more]
A0A0A0KNC40.090.56Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517... [more]
A0A5A7UBD30.089.96Serine/threonine-protein kinase HT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3AZA80.090.16serine/threonine-protein kinase HT1 OS=Cucumis melo OX=3656 GN=LOC103484517 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G58950.15.7e-16958.30Protein kinase superfamily protein [more]
AT3G46930.13.4e-12950.00Protein kinase superfamily protein [more]
AT1G62400.16.6e-9347.70Protein kinase superfamily protein [more]
AT2G24360.11.1e-7946.89Protein kinase superfamily protein [more]
AT4G31170.12.7e-7846.89Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 264..277
score: 43.0
coord: 348..358
score: 43.17
coord: 411..433
score: 37.7
coord: 367..389
score: 32.91
coord: 302..320
score: 43.41
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 191..440
e-value: 2.7E-59
score: 200.6
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 184..443
e-value: 1.2E-60
score: 217.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 184..444
score: 41.488251
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 172..268
e-value: 6.1E-20
score: 73.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 269..469
e-value: 3.0E-60
score: 205.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 100..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..140
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..498
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 479..498
NoneNo IPR availablePANTHERPTHR44329SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATEDcoord: 3..468
NoneNo IPR availablePANTHERPTHR44329:SF192TYROSINE KINASE FAMILY PROTEINcoord: 3..468
NoneNo IPR availableCDDcd13999STKc_MAP3K-likecoord: 191..440
e-value: 7.05716E-120
score: 349.529
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 308..320
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 170..440

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG10g04960Cp4.1LG10g04960gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g04960.1:exon:001Cp4.1LG10g04960.1:exon:001exon
Cp4.1LG10g04960.1:exon:002Cp4.1LG10g04960.1:exon:002exon
Cp4.1LG10g04960.1:exon:003Cp4.1LG10g04960.1:exon:003exon
Cp4.1LG10g04960.1:exon:004Cp4.1LG10g04960.1:exon:004exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g04960.1:five_prime_utr:002Cp4.1LG10g04960.1:five_prime_utr:002five_prime_UTR
Cp4.1LG10g04960.1:five_prime_utr:001Cp4.1LG10g04960.1:five_prime_utr:001five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g04960.1:cds:003Cp4.1LG10g04960.1:cds:003CDS
Cp4.1LG10g04960.1:cds:002Cp4.1LG10g04960.1:cds:002CDS
Cp4.1LG10g04960.1:cds:001Cp4.1LG10g04960.1:cds:001CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG10g04960.1:three_prime_utr:001Cp4.1LG10g04960.1:three_prime_utr:001three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG10g04960.1Cp4.1LG10g04960.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity