Cp4.1LG02g16830.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g16830.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNOD26-like intrinsic protein 1;2
LocationCp4.1LG02: 12568479 .. 12572678 (+)
Sequence length2144
RNA-Seq ExpressionCp4.1LG02g16830.1
SyntenyCp4.1LG02g16830.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCGATCTTTTCAGTTAAATTATTTTTTTAGTGTTAATACAAATTAATATGAAGTTTTCACGGACCTGTGAATTATAAATTTTAATGTTATAAAATTAATGAACTAAGCTCGATCGATTTTCATGAACCTTACGGATAACAATTGCTATAGATATATAATCGGGTCGGCAGATATAATCATCATAAACAAGTTGATTATTCTCTCGTTGTTTGTAATTATTATAATTGTATTGTAATAATCTGTTTATTATCGCTCATGTAAGTCAAAAGTCGAGCTCTATTATATAAGAGTCTGTGATACGATTATTTTTTGAAATATTAATCTCGTTAATTAAGGAATTTATAAAGATTAATGTATTTATGCAATAACGGTTTTATTTTTTATTTTTTTTATTAAGTAAATTCGTATAATATAATATATTATAAATTATATAAATAGAGTAAAATAAGTCGCAAGGAATTGTGAGAAAGCTCGTAGTTTTGTCTAGTGAAACCATAACAAACGAGGCCTTCCTGAAAAGAAGGAAGAACAGAAAGGGAAACTGATCGGAGAGCTCATAGTGAAAATTCGTGATATCGTTCCGATCGAAAGCGTTGTTTCTTCTATCGGCATCTGAGAATCAATGGCGACGAAGACCGACGGAATCCAAGAGGAAGAAATTTCAAAGCTCGAGGAAGGCACTCTCGGAACCTGCGTCGCGCGTCTCCGTCCATCGTCTTGGGTTGCTATCATCCAAAAGGTACCGCTTCAACTGCCTCTGTTATTTCTCTCTTCTGCGCGCCAATCAATTGATTGTTTGATACAACGTGTTTGTTTATCTGGAAAGTGCTGCACGTTATCTCTCGTGTGTGATAACTTGTTTTATTCTCCGGAAAATCTGGCATATGGATGAATCGACTACGATAACTGTGTTGGTTCCTATCGAAATGCAGATATTAGGTTATCGAGAGTATGAAGCTTGTTTGATTCTTTGGAAAATCTGGTACATGCATCAATCGACTGTATAAAATGTGTTAGTTTCTCTGCAAAATCTGGCACTTGAATCAATCAAGTTTTAAGTTTATTGTTAGTTTCTCCGCGAAATCTGGCACTTGAATCAATCAACTTTTAAGTTTATTGTTTGGTTCTCTGCAAATTCTGCCAAATGAACTAATCGACTGTTAGGAACTTGTTTATTTCTCTGAAAAATCTGGCTCGCAAATCAATCGACTGTATAAAACTCGTTAGTTTCTCCCCAAATGCGGCACATGAATCAATAAATTGTATAAAAATTGTTTGTTTTGCAGTAAAACCTGTTTGTTTTTCCGGAAAATGCGGTACATGGATCTGATCAATCAAGTGTATGAAATGTGTTTGTTTCTCGGAAAAATTTGTGGCAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTCTCTCTCTTCTGTTACCATCATCTCATGATAAACTTAAAGAAATTTCAACTCCAAATAGACAATTTATTTTTGGNCAAATGAACTAATCGACTGTTAGGAACTTGTTTATTTCTCTGAAAAATCTGGCTCGCAAATCAATCGACTGTATAAAACTCGTTAGTTTCTCCCCAAATGCGGCACATGAATCAATAAATTGTATAAAAATTGTTTGTTTTGCAGTAAAACCTGTTTGTTTTTCCGGAAAATGCGGTACATGGATCTGATCAATCAAGTGTATGAAATGTGTTTGTTTCTCGGAAAAATTTGTGGCAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTCTCTCTCTTCTGTTACCATCATCTCATGATAAACTTAAAGAAATTTCAACTCCAAATAGACAATTTATTTTTGGCACGAAACGCAATTATGAATATTATAGCCGAATTATATTTTCTTTGATTACATATTTCAGGTTCCGATATACATAGGAGCTCAAGTAGCAGGGTCCCTTCTTGCAAGCACCACACTGGACCTATTGTTTGAAGTGACCCCGGAAGCCTTCTTTGGGACAGTGCCGGCAGGATCCGATGTCCAATCTTTCGTCCTTGAAATCATCATCACCTTCCTCTTGCTGTTAGTCGTCTCTGGTGTCTCCACTGATCACAGAGCTGTAAGTCATCCAAAAGCTTAATAGAACAAATTAAGATTTGTAGCTTAATAGAACAAATTAAGATTTGTAGCTTAATAGAACAAATTAAGATTTGTAGCTTAATAGAACAAATTAAGATCTGTAGTTATAACTTTTTAAATACTTTTTTCTTTTTGAAATTTTGGCTGGGATTTTGGAAACCCATTGAAAAAAGGAAAAATAGAGTGTGGATCCAAGGTTCCTTCNGTTTCATTTTCTTCTTCTTCAATCTTCTCTTTTCCCTCATTATGTAATGGCCATAAATACCTTTTTTGGGGGTTTTAAATACTTCTTTTGTTTCAAAATTGTTCATTATGGATTTGATTGATTATTAATTATTTTGTTTGTAAAAAAAAAACTTTATTAAAAAAATGTTTTTATGGGCAGGATACATTTTAAAATATCCTTAATTTTACATTTTAAACTTTTTTTTTTTTAAGTAGTTTGAGAACTTTTGTTCAACAGAATTTTTTTTAAAAAAATTCGAATTTATTCTAGATTATTGAGGTTGATGGGTTGATAATATTGTAAAAATAAGCACTGTATTCATCTATATTTTCTTTAACAGGTTGGAGAATTGGCGGGAATTGTTGTTGGAATGACTGTTCTGTTGAATGTCTTCGTGGCTGGGTAACTCTAAACTTCATTTTACTGTTGATATAATTCCAACACTAAAAATTTTAAAAATTTGAAAAGTACTAAATATCAAGAATATCCATTTTTTACTCTAAACGATGGTGTTCCATGAACACTAAATATCGAGAATATCCATTTGTTTTTTTTTCTCCTATTTTAGTTATATATATATATAGACACACATAATTGTTCATGATTTAGAGAATTTGGTATGATATATTATTTTATTTCTATTAAGATGTTTAAAGGAAATGAATTTAAATGGGAAATGCTTCTCCAAGAGATTGAATTCCCACGAATTCATTGCAGGGATTANTTTTTTTTTCTTTTTTTTTTTTTTTTTTTGGTGATTATTTCTGAAAACAAAACATAGGCCGATTTCAGGAGCTTCCATGAATCCAGCTAGGACCATTGGACCTGCGATAGTGAAGGGACAGTTCAAAGGATTATGGGTTTACATGGTTGGGCCGTTGATCGGAGCTATTGCCGGAGCATTTACTTACAACCTGATGAGATTCACAGACAAGCCGTTGTCGGAGATCACCAAAAACAGCTCATTCCTCAATCGCTGCCAAAAATCATAATGCCGCCTCTTCATCATCATCATCTTATCTTTTAACTACAGAAGCCACCAAGCTCGGGAGTCTCGAGCACAGCTTCGCATTTTTTCTCTCGGTAAAGCAACTTCACTCTTGCACAATGGTCATTCATTTGTGTATTTAGTGTGTCAACCGATCAATTCATCATTCATTCATTTAGAAAATTCGTTTTAGCAAAAGCATTATTTATTTGATCAAATAGTGCTTTGTTTTTTCCTTTTTCCTTTAGTTGTTGAAATGGATTCATTGCGAGTAGGGTGATATAGAGGTTTTGTTTGGAAATTGAATCTCGAACCAGTTGGATTTTTGTTGTTTTCATGCACGTGGGGCTAAAAATTATTCAACTTTGCTCGTGATTTAGCTAGTTTGTTCTTTACGTGCATACATCTTGTATATATAACAATATACGTGGATCATGAATATTGTAAAATTGTCGATATTTGGATATCATGAAACAAAATATTCATAAGCAA

mRNA sequence

ATTCGATCTTTTCAGTTAAATTATTTTTTTAGTGTTAATACAAATTAATATGAAGTTTTCACGGACCTGTGAATTATAAATTTTAATGTTATAAAATTAATGAACTAAGCTCGATCGATTTTCATGAACCTTACGGATAACAATTGCTATAGATATATAATCGGGTCGGCAGATATAATCATCATAAACAAGTTGATTATTCTCTCGTTGTTTGTAATTATTATAATTGTATTGTAATAATCTGTTTATTATCGCTCATGTAAGTCAAAAGTCGAGCTCTATTATATAAGAGTCTGTGATACGATTATTTTTTGAAATATTAATCTCGTTAATTAAGGAATTTATAAAGATTAATGTATTTATGCAATAACGGTTTTATTTTTTATTTTTTTTATTAAGTAAATTCGTATAATATAATATATTATAAATTATATAAATAGAGTAAAATAAGTCGCAAGGAATTGTGAGAAAGCTCGTAGTTTTGTCTAGTGAAACCATAACAAACGAGGCCTTCCTGAAAAGAAGGAAGAACAGAAAGGGAAACTGATCGGAGAGCTCATAGTGAAAATTCGTGATATCGTTCCGATCGAAAGCGTTGTTTCTTCTATCGGCATCTGAGAATCAATGGCGACGAAGACCGACGGAATCCAAGAGGAAGAAATTTCAAAGCTCGAGGAAGGCACTCTCGGAACCTGCGTCGCGCGTCTCCGTCCATCGTCTTGGGTTGCTATCATCCAAAAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTTCCGATATACATAGGAGCTCAAGTAGCAGGGTCCCTTCTTGCAAGCACCACACTGGACCTATTGTTTGAAGTGACCCCGGAAGCCTTCTTTGGGACAGTGCCGGCAGGATCCGATGTCCAATCTTTCGTCCTTGAAATCATCATCACCTTCCTCTTGCTGTTAGTCGTCTCTGGTGTCTCCACTGATCACAGAGCTGTTGGAGAATTGGCGGGAATTGTTGTTGGAATGACTGTTCTGTTGAATGTCTTCGTGGCTGGGCCGATTTCAGGAGCTTCCATGAATCCAGCTAGGACCATTGGACCTGCGATAGTGAAGGGACAGTTCAAAGGATTATGGGTTTACATGGTTGGGCCGTTGATCGGAGCTATTGCCGGAGCATTTACTTACAACCTGATGAGATTCACAGACAAGCCGTTGTCGGAGATCACCAAAAACAGCTCATTCCTCAATCGCTGCCAAAAATCATAATGCCGCCTCTTCATCATCATCATCTTATCTTTTAACTACAGAAGCCACCAAGCTCGGGAGTCTCGAGCACAGCTTCGCATTTTTTCTCTCGGTAAAGCAACTTCACTCTTGCACAATGGTCATTCATTTGTGTATTTAGTGTGTCAACCGATCAATTCATCATTCATTCATTTAGAAAATTCGTTTTAGCAAAAGCATTATTTATTTGATCAAATAGTGCTTTGTTTTTTCCTTTTTCCTTTAGTTGTTGAAATGGATTCATTGCGAGTAGGGTGATATAGAGGTTTTGTTTGGAAATTGAATCTCGAACCAGTTGGATTTTTGTTGTTTTCATGCACGTGGGGCTAAAAATTATTCAACTTTGCTCGTGATTTAGCTAGTTTGTTCTTTACGTGCATACATCTTGTATATATAACAATATACGTGGATCATGAATATTGTAAAATTGTCGATATTTGGATATCATGAAACAAAATATTCATAAGCAA

Coding sequence (CDS)

ATGGCGACGAAGACCGACGGAATCCAAGAGGAAGAAATTTCAAAGCTCGAGGAAGGCACTCTCGGAACCTGCGTCGCGCGTCTCCGTCCATCGTCTTGGGTTGCTATCATCCAAAAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTTATTGCGGAGGTGATTGGAACGTACTTCGTGATCTTCACCGGATGTGGAGCGGTGGTGGTGAACAAAATTTACGGATCAGTGACGTTCCCGGGAATCTGCGTGGTTTGGGGATTGATTGTGATGGTTATGGTGTATTCGTTGGGACACATCTCTGGAGCGCACTTCAACCCTTCTGTTACCATCACATTCGCCATTTTTCGTCGCTTCCCCTTTTGTCAGGTTCCGATATACATAGGAGCTCAAGTAGCAGGGTCCCTTCTTGCAAGCACCACACTGGACCTATTGTTTGAAGTGACCCCGGAAGCCTTCTTTGGGACAGTGCCGGCAGGATCCGATGTCCAATCTTTCGTCCTTGAAATCATCATCACCTTCCTCTTGCTGTTAGTCGTCTCTGGTGTCTCCACTGATCACAGAGCTGTTGGAGAATTGGCGGGAATTGTTGTTGGAATGACTGTTCTGTTGAATGTCTTCGTGGCTGGGCCGATTTCAGGAGCTTCCATGAATCCAGCTAGGACCATTGGACCTGCGATAGTGAAGGGACAGTTCAAAGGATTATGGGTTTACATGGTTGGGCCGTTGATCGGAGCTATTGCCGGAGCATTTACTTACAACCTGATGAGATTCACAGACAAGCCGTTGTCGGAGATCACCAAAAACAGCTCATTCCTCAATCGCTGCCAAAAATCATAA

Protein sequence

MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIGTYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS
Homology
BLAST of Cp4.1LG02g16830.1 vs. ExPASy Swiss-Prot
Match: Q8W036 (Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-91
Identity = 163/226 (72.12%), Postives = 197/226 (87.17%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIY-GSVTFPGICVVWGLIVMVMVYSLGHISGAHFNP 172
           ++IAE+IGTYF+IF+GCG VVVN +Y G++TFPGICV WGLIVMVM+YS GHISGAHFNP
Sbjct: 44  KLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNP 103

Query: 173 SVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFV 232
           +VT+TFA+FRRFP+ QVP+YIGAQ+ GSLLAS TL L+F VTP+AFFGT P  S  Q+ V
Sbjct: 104 AVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQALV 163

Query: 233 LEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAI 292
            EIII+FLL+ V+SGV+TD RA GELAGI VGMT++LNVFVAGPISGASMNPAR++GPAI
Sbjct: 164 AEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAI 223

Query: 293 VKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           V G++KG+WVY+VGP +G  AG F YN MRFTDKPL E+TK++SFL
Sbjct: 224 VMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPLRELTKSASFL 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy Swiss-Prot
Match: Q9FIZ9 (Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-91
Identity = 164/232 (70.69%), Postives = 203/232 (87.50%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIY-GSVTFPGICVVWGLIVMVMVYSLGHISGAHFNP 172
           ++IAE+IGTYF++F+GCG VVVN +Y G++TFPGICV WGLIVMVM+YS GHISGAHFNP
Sbjct: 44  KLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNP 103

Query: 173 SVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFV 232
           +VT+TFAIFRRFP+ QVP+YIGAQ AGSLLAS TL L+F+VTPEAFFGT PA S  ++ V
Sbjct: 104 AVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARALV 163

Query: 233 LEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAI 292
            EIII+FLL+ V+SGV+TD+RAVGELAGI VGMT+++NVFVAGPISGASMNPAR++GPA+
Sbjct: 164 AEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPAL 223

Query: 293 VKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 344
           V G +K +WVY+VGP++G I+G F YNL+RFTDKPL E+TK++SFL     S
Sbjct: 224 VMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPLRELTKSASFLRAVSPS 275

BLAST of Cp4.1LG02g16830.1 vs. ExPASy Swiss-Prot
Match: P49173 (Probable aquaporin NIP-type OS=Nicotiana alata OX=4087 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-89
Identity = 164/225 (72.89%), Postives = 198/225 (88.00%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPS 172
           ++IAE IGTYFVIF GCG+V VNKIYGSVTFPGICV WGLIVMVMVY++G+ISGAHFNP+
Sbjct: 44  KLIAEAIGTYFVIFAGCGSVAVNKIYGSVTFPGICVTWGLIVMVMVYTVGYISGAHFNPA 103

Query: 173 VTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVL 232
           VTITF+IF RFP+ QVP+YI AQ+ GS+LAS TL LLF+VTP+A+FGTVP GS+ QS  +
Sbjct: 104 VTITFSIFGRFPWKQVPLYIIAQLMGSILASGTLALLFDVTPQAYFGTVPVGSNGQSLAI 163

Query: 233 EIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIV 292
           EIII+FLL+ V+SGV+TD RA+G++AGI VGMT+ LNVFVAGPISGASMNPAR+IGPAIV
Sbjct: 164 EIIISFLLMFVISGVATDDRAIGQVAGIAVGMTITLNVFVAGPISGASMNPARSIGPAIV 223

Query: 293 KGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           K  + GLWVY+VGP+IG +AGAF YNL+R TDKPL E+ K++S L
Sbjct: 224 KHVYTGLWVYVVGPIIGTLAGAFVYNLIRSTDKPLRELAKSASSL 268

BLAST of Cp4.1LG02g16830.1 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 5.1e-81
Identity = 156/257 (60.70%), Postives = 194/257 (75.49%), Query Frame = 0

Query: 88  GHISGAHFNPSVTITFAIFRRFPFCQVIAEVIGTYFVIFTGCGAVVVNK-IYGSVTFPGI 147
           G  S ++ +P  ++ FA        +VIAE++GT+F+IF GC AV VNK   G+VTFPGI
Sbjct: 37  GSKSSSNNHPMFSVQFA-------QKVIAEILGTFFLIFAGCAAVAVNKRTGGTVTFPGI 96

Query: 148 CVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTL 207
           C+ WGL VMVMVYS+GHISGAH NP+VT+ FA   RFP+ +VP Y  AQVAGS  AS  L
Sbjct: 97  CITWGLAVMVMVYSVGHISGAHLNPAVTLAFATCGRFPWRRVPAYAAAQVAGSAAASAAL 156

Query: 208 DLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTV 267
             LF   PE FFGT PAGSDVQS  +E IITF L+ VVSGV+TD+RA+GELAG+ VG TV
Sbjct: 157 RALFGGAPEHFFGTAPAGSDVQSLAMEFIITFYLMFVVSGVATDNRAIGELAGLAVGATV 216

Query: 268 LLNVFVAGPISGASMNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKP 327
           L+NV  AGPISGASMNPARTIGPAI+ G++ G+WVY+ GP+ GA+AGA+ YNL+RFTDKP
Sbjct: 217 LVNVLFAGPISGASMNPARTIGPAIILGRYTGIWVYIAGPVFGAVAGAWAYNLIRFTDKP 276

Query: 328 LSEITKNSSFLNRCQKS 344
           L EIT  +SF+   +++
Sbjct: 277 LREITMTASFIRSTRRN 286

BLAST of Cp4.1LG02g16830.1 vs. ExPASy Swiss-Prot
Match: Q40746 (Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.6e-80
Identity = 147/230 (63.91%), Postives = 189/230 (82.17%), Query Frame = 0

Query: 110 PFCQ-VIAEVIGTYFVIFTGCGAVVVNKI-YGSVTFPGICVVWGLIVMVMVYSLGHISGA 169
           PF Q +IAE+ GTYF+IF GCGAV +N+   G +TFPG+ +VWGL VMVMVY++GHISGA
Sbjct: 45  PFIQKIIAEIFGTYFLIFAGCGAVTINQSKNGQITFPGVAIVWGLAVMVMVYAVGHISGA 104

Query: 170 HFNPSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDV 229
           HFNP+VT+ FA  RRFP+ QVP Y  AQ+ G+ LA+ TL L+F    E F GT+PAGSDV
Sbjct: 105 HFNPAVTLAFATCRRFPWRQVPAYAAAQMLGATLAAGTLRLMFGGRHEHFPGTLPAGSDV 164

Query: 230 QSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTI 289
           QS VLE IITF L+ V+SGV+TD+RA+GELAG+ VG T+LLNV +AGPISGASMNPAR++
Sbjct: 165 QSLVLEFIITFYLMFVISGVATDNRAIGELAGLAVGATILLNVLIAGPISGASMNPARSL 224

Query: 290 GPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           GPA++ G+++ +WVY+VGP+ GA+AGA+ YN++RFT+KPL EITK+ SFL
Sbjct: 225 GPAMIGGEYRSIWVYIVGPVAGAVAGAWAYNIIRFTNKPLREITKSGSFL 274

BLAST of Cp4.1LG02g16830.1 vs. NCBI nr
Match: XP_023523665.1 (probable aquaporin NIP-type [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486 bits (1252), Expect = 3.40e-171
Identity = 269/343 (78.43%), Postives = 269/343 (78.43%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQ       
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQ------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                  VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL
Sbjct: 181 -------VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW
Sbjct: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS
Sbjct: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 269

BLAST of Cp4.1LG02g16830.1 vs. NCBI nr
Match: XP_022948505.1 (putative aquaporin NIP4-1 [Cucurbita moschata] >KAG6607007.1 putative aquaporin NIP4-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036706.1 putative aquaporin NIP4-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 480 bits (1236), Expect = 9.29e-169
Identity = 266/343 (77.55%), Postives = 268/343 (78.13%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYSLGH+SGAHFNPSVTITFAIFRRFPFCQ       
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHVSGAHFNPSVTITFAIFRRFPFCQ------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                  VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL
Sbjct: 181 -------VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW
Sbjct: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITK+SSFLNR QKS
Sbjct: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of Cp4.1LG02g16830.1 vs. NCBI nr
Match: XP_022998713.1 (probable aquaporin NIP-type [Cucurbita maxima])

HSP 1 Score: 463 bits (1191), Expect = 6.58e-162
Identity = 262/343 (76.38%), Postives = 263/343 (76.68%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATKTDGIQEEEISKLEEGTLGT VARLRPSS V  IQKVIAEVIGTYFVIFTGCGAVVV
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTGVARLRPSSCVVTIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPF Q       
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFRQ------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                  VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL
Sbjct: 181 -------VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW
Sbjct: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITK+SSFLNR QKS
Sbjct: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of Cp4.1LG02g16830.1 vs. NCBI nr
Match: CAA3015332.1 (Probable aquaporin NIP-type [Olea europaea subsp. europaea])

HSP 1 Score: 430 bits (1106), Expect = 9.37e-148
Identity = 230/352 (65.34%), Postives = 276/352 (78.41%), Query Frame = 0

Query: 13  ISKLEEG----TLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVVNKIYG-SV 72
           I ++EEG    T          +S V I+QKVIAEVIGTYFVIF+GCG+V VNK+YG +V
Sbjct: 3   IEEMEEGVDTSTFNAKEGFCMSASIVTIVQKVIAEVIGTYFVIFSGCGSVAVNKLYGGTV 62

Query: 73  TFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQV------------- 132
           TFPGIC  WGLIVMVMVYS+GHISGAHFNP+VTITFAIFRRFP+ +V             
Sbjct: 63  TFPGICATWGLIVMVMVYSVGHISGAHFNPAVTITFAIFRRFPWKEVPINTTDMFNMLDR 122

Query: 133 ---IAEVIGTYFVIFTGCGAVVVNKIYG-SVTFPGICVVWGLIVMVMVYSLGHISGAHFN 192
              IAEVIGTYFVIF+GCG+V VNK+YG +VTFPGIC  WGLIVMVMVYS+GHISGAHFN
Sbjct: 123 FHVIAEVIGTYFVIFSGCGSVAVNKLYGGTVTFPGICATWGLIVMVMVYSVGHISGAHFN 182

Query: 193 PSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSF 252
           P+VTITF          VP+YI AQ+ GS+LAS TL +LF++T EA+FGT+P GS+ QSF
Sbjct: 183 PAVTITF----------VPLYIIAQLIGSILASCTLAILFDITSEAYFGTLPVGSNGQSF 242

Query: 253 VLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPA 312
            +EIII+FLL+  +SGV+TD RA+G+LAG+VVGMT++LNVFVAGPISGASMNPAR+IGPA
Sbjct: 243 AIEIIISFLLMFTISGVATDSRAIGDLAGLVVGMTIMLNVFVAGPISGASMNPARSIGPA 302

Query: 313 IVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQK 342
           IVK  +KGLWVY+ GPLIG I G F YNL+R+T+KPL E+TK+SS +    K
Sbjct: 303 IVKHTYKGLWVYIFGPLIGTILGGFAYNLIRYTNKPLRELTKSSSLIRSISK 344

BLAST of Cp4.1LG02g16830.1 vs. NCBI nr
Match: XP_038882565.1 (probable aquaporin NIP-type [Benincasa hispida])

HSP 1 Score: 398 bits (1023), Expect = 2.38e-136
Identity = 222/343 (64.72%), Postives = 244/343 (71.14%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATK DGI+EEEISKLEEGTL + VARL PS+ V IIQKVIAEVIGTYFVIF GCGAV V
Sbjct: 1   MATKIDGIEEEEISKLEEGTLVSAVARLCPSTSVVIIQKVIAEVIGTYFVIFAGCGAVAV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYS+GHISGAHFNP+VT+TFA FRRFPF         
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSVGHISGAHFNPAVTLTFAFFRRFPF--------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                        W                              
Sbjct: 121 -----------------------------W------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                 QVPIY+GAQ+ GSLLAS TL+L+ EVTPEAFFGT P GS++QS VLEIIITFLL
Sbjct: 181 ------QVPIYVGAQLMGSLLASCTLELMLEVTPEAFFGTEPVGSNLQSLVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           + V+SGVSTD+RAVGE AGIVVGMT+LLNVFVAGPISGASMNPAR+IGPAIVK QFKGLW
Sbjct: 241 MFVISGVSTDNRAVGEFAGIVVGMTILLNVFVAGPISGASMNPARSIGPAIVKRQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VY+VGPLIGA+AG F YNL+RFTDKPL EIT++SSF    QKS
Sbjct: 301 VYVVGPLIGAVAGGFVYNLIRFTDKPLGEITRSSSFFTGSQKS 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy TrEMBL
Match: A0A6J1GA14 (putative aquaporin NIP4-1 OS=Cucurbita moschata OX=3662 GN=LOC111452164 PE=3 SV=1)

HSP 1 Score: 480 bits (1236), Expect = 4.50e-169
Identity = 266/343 (77.55%), Postives = 268/343 (78.13%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYSLGH+SGAHFNPSVTITFAIFRRFPFCQ       
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHVSGAHFNPSVTITFAIFRRFPFCQ------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                  VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL
Sbjct: 181 -------VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW
Sbjct: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITK+SSFLNR QKS
Sbjct: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy TrEMBL
Match: A0A6J1KHI9 (probable aquaporin NIP-type OS=Cucurbita maxima OX=3661 GN=LOC111493295 PE=3 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 3.19e-162
Identity = 262/343 (76.38%), Postives = 263/343 (76.68%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATKTDGIQEEEISKLEEGTLGT VARLRPSS V  IQKVIAEVIGTYFVIFTGCGAVVV
Sbjct: 1   MATKTDGIQEEEISKLEEGTLGTGVARLRPSSCVVTIQKVIAEVIGTYFVIFTGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPF Q       
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFRQ------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                  VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL
Sbjct: 181 -------VPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW
Sbjct: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITK+SSFLNR QKS
Sbjct: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKSSSFLNRSQKS 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy TrEMBL
Match: A0A5A7V2N2 (Putative aquaporin NIP-type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold72G00660 PE=3 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 1.33e-135
Identity = 219/343 (63.85%), Postives = 244/343 (71.14%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATK DGI++EEISKLEEGT+ + +ARL PS+ V IIQKVIAE+IGTYFVIF+GCGAV V
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYS+GH+SGAHFNP+VT TFA+FRRFPF         
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTFTFALFRRFPF--------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                        W                              
Sbjct: 121 -----------------------------W------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                 QVPIY GAQ+ GSLLAS TLDL+ EVTPEAFFGTVP GS+VQS VLEIIITFLL
Sbjct: 181 ------QVPIYTGAQLMGSLLASCTLDLMLEVTPEAFFGTVPVGSNVQSLVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           + V+SGVSTD+RAVGEL GIVVGMT+LLNVFVAGPISGASMNPARTIGPAIVK QFKGLW
Sbjct: 241 MFVISGVSTDNRAVGELGGIVVGMTILLNVFVAGPISGASMNPARTIGPAIVKRQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VY+VGP IGA+AG F YNLMR+TDKPL EIT+++SFL    KS
Sbjct: 301 VYIVGPFIGAVAGGFVYNLMRYTDKPLREITRSTSFLTGTLKS 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy TrEMBL
Match: A0A1S3C5C4 (probable aquaporin NIP-type OS=Cucumis melo OX=3656 GN=LOC103496839 PE=3 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 1.33e-135
Identity = 219/343 (63.85%), Postives = 244/343 (71.14%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATK DGI++EEISKLEEGT+ + +ARL PS+ V IIQKVIAE+IGTYFVIF+GCGAV V
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYS+GH+SGAHFNP+VT TFA+FRRFPF         
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTFTFALFRRFPF--------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                        W                              
Sbjct: 121 -----------------------------W------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                 QVPIY GAQ+ GSLLAS TLDL+ EVTPEAFFGTVP GS+VQS VLEIIITFLL
Sbjct: 181 ------QVPIYTGAQLMGSLLASCTLDLMLEVTPEAFFGTVPVGSNVQSLVLEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           + V+SGVSTD+RAVGEL GIVVGMT+LLNVFVAGPISGASMNPARTIGPAIVK QFKGLW
Sbjct: 241 MFVISGVSTDNRAVGELGGIVVGMTILLNVFVAGPISGASMNPARTIGPAIVKRQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VY+VGP IGA+AG F YNLMR+TDKPL EIT+++SFL    KS
Sbjct: 301 VYIVGPFIGAVAGGFVYNLMRYTDKPLREITRSTSFLTGTLKS 269

BLAST of Cp4.1LG02g16830.1 vs. ExPASy TrEMBL
Match: A0A0A0LA39 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G345890 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 8.89e-134
Identity = 215/343 (62.68%), Postives = 244/343 (71.14%), Query Frame = 0

Query: 1   MATKTDGIQEEEISKLEEGTLGTCVARLRPSSWVAIIQKVIAEVIGTYFVIFTGCGAVVV 60
           MATK DGI++EEISKLEEG + + VAR  PS+ V IIQKVIAE+IGTYFVIF GCGAVVV
Sbjct: 1   MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 60

Query: 61  NKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIFRRFPFCQVIAEVIG 120
           NKIYGSVTFPGICVVWGLIVMVMVYS+GH+SGAHFNP+VT+TFA+FRRFPF         
Sbjct: 61  NKIYGSVTFPGICVVWGLIVMVMVYSVGHVSGAHFNPAVTLTFALFRRFPF--------- 120

Query: 121 TYFVIFTGCGAVVVNKIYGSVTFPGICVVWGLIVMVMVYSLGHISGAHFNPSVTITFAIF 180
                                        W                              
Sbjct: 121 -----------------------------W------------------------------ 180

Query: 181 RRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFVLEIIITFLL 240
                 QVPIY GAQ+ GSLLAS TLDL+FEVTPEAFFGTVP GS+VQS V+EIIITFLL
Sbjct: 181 ------QVPIYTGAQLMGSLLASCTLDLMFEVTPEAFFGTVPVGSNVQSLVIEIIITFLL 240

Query: 241 LLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAIVKGQFKGLW 300
           + V+SGVSTD+RAVGEL G++VGMT+LLNVFVAGPISGASMNPAR++GPAIVK QFKGLW
Sbjct: 241 MFVISGVSTDNRAVGELGGVMVGMTILLNVFVAGPISGASMNPARSLGPAIVKRQFKGLW 269

Query: 301 VYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 343
           VYM+GPLIGA+AG F YNLMR+TDK L EIT+++SFL    KS
Sbjct: 301 VYMLGPLIGAVAGGFVYNLMRYTDKSLREITRSTSFLTGTHKS 269

BLAST of Cp4.1LG02g16830.1 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 337.4 bits (864), Expect = 1.3e-92
Identity = 163/226 (72.12%), Postives = 197/226 (87.17%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIY-GSVTFPGICVVWGLIVMVMVYSLGHISGAHFNP 172
           ++IAE+IGTYF+IF+GCG VVVN +Y G++TFPGICV WGLIVMVM+YS GHISGAHFNP
Sbjct: 44  KLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNP 103

Query: 173 SVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFV 232
           +VT+TFA+FRRFP+ QVP+YIGAQ+ GSLLAS TL L+F VTP+AFFGT P  S  Q+ V
Sbjct: 104 AVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQALV 163

Query: 233 LEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAI 292
            EIII+FLL+ V+SGV+TD RA GELAGI VGMT++LNVFVAGPISGASMNPAR++GPAI
Sbjct: 164 AEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAI 223

Query: 293 VKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           V G++KG+WVY+VGP +G  AG F YN MRFTDKPL E+TK++SFL
Sbjct: 224 VMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPLRELTKSASFL 269

BLAST of Cp4.1LG02g16830.1 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 337.0 bits (863), Expect = 1.7e-92
Identity = 164/232 (70.69%), Postives = 203/232 (87.50%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIY-GSVTFPGICVVWGLIVMVMVYSLGHISGAHFNP 172
           ++IAE+IGTYF++F+GCG VVVN +Y G++TFPGICV WGLIVMVM+YS GHISGAHFNP
Sbjct: 44  KLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNP 103

Query: 173 SVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTPEAFFGTVPAGSDVQSFV 232
           +VT+TFAIFRRFP+ QVP+YIGAQ AGSLLAS TL L+F+VTPEAFFGT PA S  ++ V
Sbjct: 104 AVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARALV 163

Query: 233 LEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPARTIGPAI 292
            EIII+FLL+ V+SGV+TD+RAVGELAGI VGMT+++NVFVAGPISGASMNPAR++GPA+
Sbjct: 164 AEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPAL 223

Query: 293 VKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFLNRCQKS 344
           V G +K +WVY+VGP++G I+G F YNL+RFTDKPL E+TK++SFL     S
Sbjct: 224 VMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPLRELTKSASFLRAVSPS 275

BLAST of Cp4.1LG02g16830.1 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 144/237 (60.76%), Postives = 187/237 (78.90%), Query Frame = 0

Query: 110 PFCQ-VIAEVIGTYFVIFTGCGAVVVNKIYG-SVTFPGICVVWGLIVMVMVYSLGHISGA 169
           PF Q ++AEV+GTYF+IF GC AV VN  +  +VT PGI +VWGL VMV+VYSLGHISGA
Sbjct: 49  PFLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGA 108

Query: 170 HFNPSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTP-------EAFFGT 229
           HFNP+VTI FA   RFP  QVP Y+ +QV GS LA+ TL LLF +         + F GT
Sbjct: 109 HFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGT 168

Query: 230 VPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGAS 289
           +P+GS++QSFV+E IITF L+ V+SGV+TD+RA+GELAG+ VG TVLLNV +AGP+SGAS
Sbjct: 169 LPSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGAS 228

Query: 290 MNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           MNP R++GPA+V   ++GLW+Y+V P++GA++GA+ YN++R+TDKPL EITK+ SFL
Sbjct: 229 MNPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPLREITKSGSFL 285

BLAST of Cp4.1LG02g16830.1 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-73
Identity = 137/237 (57.81%), Postives = 180/237 (75.95%), Query Frame = 0

Query: 110 PFCQ-VIAEVIGTYFVIFTGCGAVVVNKIYGS-VTFPGICVVWGLIVMVMVYSLGHISGA 169
           PF Q +IAE +GTYF++FTGC +VVVN    + VT PGI +VWGL +MV++YSLGHISGA
Sbjct: 52  PFLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGA 111

Query: 170 HFNPSVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEV-------TPEAFFGT 229
           H NP+VTI FA   RFP  QVP Y+ +QV GS LA+ TL LLF +         + F G+
Sbjct: 112 HINPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHDVCSGKHDVFIGS 171

Query: 230 VPAGSDVQSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGAS 289
            P GSD+Q+F +E I+TF L+ ++SGV+TD+RA+GELAG+ +G TVLLNV +A P+S AS
Sbjct: 172 SPVGSDLQAFTMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLLNVLIAAPVSSAS 231

Query: 290 MNPARTIGPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITKNSSFL 338
           MNP R++GPA+V G +KG+W+Y+V P +GAIAGA+ YN +R+TDKPL EITK+ SFL
Sbjct: 232 MNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTDKPLREITKSGSFL 288

BLAST of Cp4.1LG02g16830.1 vs. TAIR 10
Match: AT1G31885.1 (NOD26-like intrinsic protein 3;1 )

HSP 1 Score: 241.5 bits (615), Expect = 9.9e-64
Identity = 120/228 (52.63%), Postives = 167/228 (73.25%), Query Frame = 0

Query: 113 QVIAEVIGTYFVIFTGCGAVVVNKIYGS-VTFPGICVVWGLIVMVMVYSLGHISGAHFNP 172
           ++I E +GT+ +IF GC A+VVN+ YG  VT PGI +VWGL+V VM+YS+GH+SGAHFNP
Sbjct: 44  KLIGEFVGTFTMIFAGCSAIVVNETYGKPVTLPGIALVWGLVVTVMIYSIGHVSGAHFNP 103

Query: 173 SVTITFAIFRRFPFCQVPIYIGAQVAGSLLASTTLDLLFEVTP-------EAFFGTVPAG 232
           +V+I FA  ++FPF QVP YI AQ+ GS LA+  L L+F +         + + GT P+ 
Sbjct: 104 AVSIAFASSKKFPFNQVPGYIAAQLLGSTLAAAVLRLVFHLDDDVCSLKGDVYVGTYPSN 163

Query: 233 SDVQSFVLEIIITFLLLLVVSGVSTDHRAVGELAGIVVGMTVLLNVFVAGPISGASMNPA 292
           S+  SFV+E I TF L+ V+S V+TD RA G  AGI +G T++L++  +GPISGASMNPA
Sbjct: 164 SNTTSFVMEFIATFNLMFVISAVATDKRATGSFAGIAIGATIVLDILFSGPISGASMNPA 223

Query: 293 RTIGPAIVKGQFKGLWVYMVGPLIGAIAGAFTYNLMRFTDKPLSEITK 333
           R++GPA++ G +K LW+Y+V P+IGA++GA+TY L+R T K  SEI +
Sbjct: 224 RSLGPALIWGCYKDLWLYIVSPVIGALSGAWTYGLLRSTKKSYSEIIR 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W0361.9e-9172.12Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2[more]
Q9FIZ92.4e-9170.69Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1[more]
P491731.3e-8972.89Probable aquaporin NIP-type OS=Nicotiana alata OX=4087 PE=2 SV=1[more]
Q0DK165.1e-8160.70Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2[more]
Q407469.6e-8063.91Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023523665.13.40e-17178.43probable aquaporin NIP-type [Cucurbita pepo subsp. pepo][more]
XP_022948505.19.29e-16977.55putative aquaporin NIP4-1 [Cucurbita moschata] >KAG6607007.1 putative aquaporin ... [more]
XP_022998713.16.58e-16276.38probable aquaporin NIP-type [Cucurbita maxima][more]
CAA3015332.19.37e-14865.34Probable aquaporin NIP-type [Olea europaea subsp. europaea][more]
XP_038882565.12.38e-13664.72probable aquaporin NIP-type [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GA144.50e-16977.55putative aquaporin NIP4-1 OS=Cucurbita moschata OX=3662 GN=LOC111452164 PE=3 SV=... [more]
A0A6J1KHI93.19e-16276.38probable aquaporin NIP-type OS=Cucurbita maxima OX=3661 GN=LOC111493295 PE=3 SV=... [more]
A0A5A7V2N21.33e-13563.85Putative aquaporin NIP-type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3C5C41.33e-13563.85probable aquaporin NIP-type OS=Cucumis melo OX=3656 GN=LOC103496839 PE=3 SV=1[more]
A0A0A0LA398.89e-13462.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G345890 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G37820.11.3e-9272.12NOD26-like intrinsic protein 4;2 [more]
AT5G37810.11.7e-9270.69NOD26-like intrinsic protein 4;1 [more]
AT4G18910.13.0e-7660.76NOD26-like intrinsic protein 1;2 [more]
AT4G19030.15.2e-7357.81NOD26-like major intrinsic protein 1 [more]
AT1G31885.19.9e-6452.63NOD26-like intrinsic protein 3;1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 187..206
score: 30.53
coord: 300..320
score: 39.97
coord: 232..250
score: 33.37
coord: 263..285
score: 41.54
coord: 112..131
score: 34.16
coord: 150..174
score: 49.26
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 116..317
e-value: 2.7E-54
score: 182.3
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 34..115
e-value: 3.8E-19
score: 69.3
coord: 113..317
e-value: 1.3E-55
score: 188.6
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 113..320
e-value: 5.78546E-53
score: 172.438
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 114..334
e-value: 2.0E-66
score: 225.5
coord: 5..113
e-value: 1.8E-23
score: 84.6
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 36..115
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 113..331
NoneNo IPR availablePANTHERPTHR45724:SF6AQUAPORIN NIP4-1-RELATEDcoord: 113..333
NoneNo IPR availablePANTHERPTHR45724:SF6AQUAPORIN NIP4-1-RELATEDcoord: 28..115
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 28..115
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 113..333
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 168..176
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 94..102

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG02g16830Cp4.1LG02g16830gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG02g16830.1:five_prime_utr:001Cp4.1LG02g16830.1:five_prime_utr:001five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG02g16830.1:exon:001Cp4.1LG02g16830.1:exon:001exon
Cp4.1LG02g16830.1:exon:002Cp4.1LG02g16830.1:exon:002exon
Cp4.1LG02g16830.1:exon:003Cp4.1LG02g16830.1:exon:003exon
Cp4.1LG02g16830.1:exon:004Cp4.1LG02g16830.1:exon:004exon
Cp4.1LG02g16830.1:exon:005Cp4.1LG02g16830.1:exon:005exon
Cp4.1LG02g16830.1:exon:006Cp4.1LG02g16830.1:exon:006exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG02g16830.1:cds:006Cp4.1LG02g16830.1:cds:006CDS
Cp4.1LG02g16830.1:cds:005Cp4.1LG02g16830.1:cds:005CDS
Cp4.1LG02g16830.1:cds:004Cp4.1LG02g16830.1:cds:004CDS
Cp4.1LG02g16830.1:cds:003Cp4.1LG02g16830.1:cds:003CDS
Cp4.1LG02g16830.1:cds:002Cp4.1LG02g16830.1:cds:002CDS
Cp4.1LG02g16830.1:cds:001Cp4.1LG02g16830.1:cds:001CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG02g16830.1:three_prime_utr:001Cp4.1LG02g16830.1:three_prime_utr:001three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG02g16830.1Cp4.1LG02g16830.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015267 channel activity