CmoCh16G006580.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G006580.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	DUF4228 domain-containing protein
Location	Cmo_Chr16: 3250160 .. 3250834 (+)
Sequence length	675
RNA-Seq Expression	CmoCh16G006580.1
Synteny	CmoCh16G006580.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAACTGCTTATTCGGCGGTGGGTCTGGTGAGATTCAGGGGAAAATCAAGGTAATCACGTCCAATGGCGGGATTATGGAGTTGGGTTCTCCGATTACCGTCGGGTGCATCGCCGATGAGTTTCCTGGCTATGGAATATTCAAAAGCCAAGATCTTTTTTGGAACCCATTACCGCACAACGAGGAGCTGCTTCCGGGGAAATCCTACTACTTGCTTCAGAGAAACAGGGGAAGAAACAGAGGAGAGACAGAAGAAGGGGAAATGGGAATGATAAGGGCGCGTGAGGGGCACGTGAGGTCGAATAGTGTACCGGAGGCGACGGCGGCGGCGGCGGCGGGTATGGCGCCGTACAGAATGTCGTTTGATTATCAGGGGGTTTTGAGGAGGTCGCAGACGGAGGTTTTTTCGAGGAGCAGTGAGAAGAACGGCGGGGGGGTTTGGAAGGTGAAATTGGTGATTAGTCCGAAGCGATTGGTGGAGATTTTGGAGGAGGAAGGTCACACTCAGGAGTTGATTGAGAGCGTAAGGACTGTGGCTAAATGTGGAAGTACTAGCACCAGCAGCAGCTTTTCGTCGTCCATGGCGTTTTCCGATCACTGGAGTTTGTCCTCCACCACCGCCAATGCTACTCCGAGCGCTTCCTCCAAAAGTGGCGGCTTGCCGGAGATCTAA

mRNA sequence

ATGGGGAACTGCTTATTCGGCGGTGGGTCTGGTGAGATTCAGGGGAAAATCAAGGTAATCACGTCCAATGGCGGGATTATGGAGTTGGGTTCTCCGATTACCGTCGGGTGCATCGCCGATGAGTTTCCTGGCTATGGAATATTCAAAAGCCAAGATCTTTTTTGGAACCCATTACCGCACAACGAGGAGCTGCTTCCGGGGAAATCCTACTACTTGCTTCAGAGAAACAGGGGAAGAAACAGAGGAGAGACAGAAGAAGGGGAAATGGGAATGATAAGGGCGCGTGAGGGGCACGTGAGGTCGAATAGTGTACCGGAGGCGACGGCGGCGGCGGCGGCGGGTATGGCGCCGTACAGAATGTCGTTTGATTATCAGGGGGTTTTGAGGAGGTCGCAGACGGAGGTTTTTTCGAGGAGCAGTGAGAAGAACGGCGGGGGGGTTTGGAAGGTGAAATTGGTGATTAGTCCGAAGCGATTGGTGGAGATTTTGGAGGAGGAAGGTCACACTCAGGAGTTGATTGAGAGCGTAAGGACTGTGGCTAAATGTGGAAGTACTAGCACCAGCAGCAGCTTTTCGTCGTCCATGGCGTTTTCCGATCACTGGAGTTTGTCCTCCACCACCGCCAATGCTACTCCGAGCGCTTCCTCCAAAAGTGGCGGCTTGCCGGAGATCTAA

Coding sequence (CDS)

ATGGGGAACTGCTTATTCGGCGGTGGGTCTGGTGAGATTCAGGGGAAAATCAAGGTAATCACGTCCAATGGCGGGATTATGGAGTTGGGTTCTCCGATTACCGTCGGGTGCATCGCCGATGAGTTTCCTGGCTATGGAATATTCAAAAGCCAAGATCTTTTTTGGAACCCATTACCGCACAACGAGGAGCTGCTTCCGGGGAAATCCTACTACTTGCTTCAGAGAAACAGGGGAAGAAACAGAGGAGAGACAGAAGAAGGGGAAATGGGAATGATAAGGGCGCGTGAGGGGCACGTGAGGTCGAATAGTGTACCGGAGGCGACGGCGGCGGCGGCGGCGGGTATGGCGCCGTACAGAATGTCGTTTGATTATCAGGGGGTTTTGAGGAGGTCGCAGACGGAGGTTTTTTCGAGGAGCAGTGAGAAGAACGGCGGGGGGGTTTGGAAGGTGAAATTGGTGATTAGTCCGAAGCGATTGGTGGAGATTTTGGAGGAGGAAGGTCACACTCAGGAGTTGATTGAGAGCGTAAGGACTGTGGCTAAATGTGGAAGTACTAGCACCAGCAGCAGCTTTTCGTCGTCCATGGCGTTTTCCGATCACTGGAGTTTGTCCTCCACCACCGCCAATGCTACTCCGAGCGCTTCCTCCAAAAGTGGCGGCTTGCCGGAGATCTAA

Protein sequence

MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPHNEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRMSFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVAKCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI

Homology

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BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL
Match: A0A6J1ETY6 (uncharacterized protein LOC111437546 OS=Cucurbita moschata OX=3662 GN=LOC111437546 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.2e-119
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60
           MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH
Sbjct: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60

Query: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120
           NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM
Sbjct: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120

Query: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180
           SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA
Sbjct: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180

Query: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 225
           KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI
Sbjct: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 224

[Close]

BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL
Match: A0A6J1JCF7 (uncharacterized protein LOC111483212 OS=Cucurbita maxima OX=3661 GN=LOC111483212 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 3.8e-112
Identity = 218/224 (97.32%), Postives = 219/224 (97.77%), Query Frame = 0


Query: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60
           MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKS DLFWNPLPH
Sbjct: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSHDLFWNPLPH 60

Query: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120
           NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEAT AAAAGMA YRM
Sbjct: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEAT-AAAAGMASYRM 120

Query: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180
           SFDYQGVLRRSQTEVFSRSSEKN GGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA
Sbjct: 121 SFDYQGVLRRSQTEVFSRSSEKN-GGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180

Query: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 225
           KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSAS+KSGGL EI
Sbjct: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASAKSGGLLEI 222

[Close]

BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL
Match: A0A0A0KUU6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047890 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.2e-103
Identity = 204/224 (91.07%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60
           MGNCLF GG+GEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKS DLFWNPLPH
Sbjct: 1   MGNCLFAGGAGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSHDLFWNPLPH 60

Query: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120
           NEELLPGKSYYLL RNRGRNRG  +  EMG+IRAREGHVRSNSVPEA AAA A MAPYRM
Sbjct: 61  NEELLPGKSYYLLPRNRGRNRGGEDGVEMGIIRAREGHVRSNSVPEA-AAAMAAMAPYRM 120

Query: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180
           SFDYQGVLRRSQTEVFSR SEKN GGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA
Sbjct: 121 SFDYQGVLRRSQTEVFSRYSEKN-GGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180

Query: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 225
           KCGSTSTSSSFSSSMAFSD WSLS+ TANATPS SSKSGGL EI
Sbjct: 181 KCGSTSTSSSFSSSMAFSDQWSLSTATANATPSVSSKSGGLLEI 222

[Close]

BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL
Match: A0A5D3D1S5 (DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004760 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.5e-103
Identity = 203/224 (90.62%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60
           MGNCLF GG+GEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKS DLFWNPLPH
Sbjct: 1   MGNCLFAGGAGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSHDLFWNPLPH 60

Query: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120
           NEELLPGKSYYLL RNRGRNRG  +  EMG+IRAREGHVRSNSVPEA AAA A MAPYRM
Sbjct: 61  NEELLPGKSYYLLPRNRGRNRGGEDGEEMGIIRAREGHVRSNSVPEA-AAAMAAMAPYRM 120

Query: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180
           SFDYQGVLRRSQTEVFSR SEKN GGVWKVKLVISP+RLVEILEEEGHTQELIESVRTVA
Sbjct: 121 SFDYQGVLRRSQTEVFSRCSEKN-GGVWKVKLVISPRRLVEILEEEGHTQELIESVRTVA 180

Query: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 225
           KCGSTSTSSSFSSSMAFSD WSLS+ TANATPS SSKSGGL EI
Sbjct: 181 KCGSTSTSSSFSSSMAFSDQWSLSTATANATPSVSSKSGGLLEI 222

[Close]

BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL
Match: A0A1S3BSZ8 (uncharacterized protein LOC103493172 OS=Cucumis melo OX=3656 GN=LOC103493172 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.5e-103
Identity = 203/224 (90.62%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MGNCLFGGGSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLPH 60
           MGNCLF GG+GEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKS DLFWNPLPH
Sbjct: 1   MGNCLFAGGAGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSHDLFWNPLPH 60

Query: 61  NEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYRM 120
           NEELLPGKSYYLL RNRGRNRG  +  EMG+IRAREGHVRSNSVPEA AAA A MAPYRM
Sbjct: 61  NEELLPGKSYYLLPRNRGRNRGGEDGEEMGIIRAREGHVRSNSVPEA-AAAMAAMAPYRM 120

Query: 121 SFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILEEEGHTQELIESVRTVA 180
           SFDYQGVLRRSQTEVFSR SEKN GGVWKVKLVISP+RLVEILEEEGHTQELIESVRTVA
Sbjct: 121 SFDYQGVLRRSQTEVFSRCSEKN-GGVWKVKLVISPRRLVEILEEEGHTQELIESVRTVA 180

Query: 181 KCGSTSTSSSFSSSMAFSDHWSLSSTTANATPSASSKSGGLPEI 225
           KCGSTSTSSSFSSSMAFSD WSLS+ TANATPS SSKSGGL EI
Sbjct: 181 KCGSTSTSSSFSSSMAFSDQWSLSTATANATPSVSSKSGGLLEI 222

[Close]

BLAST of CmoCh16G006580.1 vs. TAIR 10
Match: AT1G64700.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 11 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G61920.1); Has 48 Blast hits to 47 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 48; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 138.7 bits (348), Expect = 5.9e-33
Identity = 92/210 (43.81%), Postives = 122/210 (58.10%), Query Frame = 0


Query: 1   MGNCLFGG-GSGEIQGKIKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLFWNPLP 60
           MGNCLFGG G  E    IKVI S+GG++E  SP+T G ++  F G+ +F + DL W PL 
Sbjct: 1   MGNCLFGGLGDEEEDLLIKVIKSDGGVLEFYSPVTAGFVSHGFSGHALFSAVDLLWKPLA 60

Query: 61  HNEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAAAGMAPYR 120
           H+  L+PG+SYYL   N   +  +T  G          HVRSNS       + + + PYR
Sbjct: 61  HDHLLVPGQSYYLFP-NIVSDELKTFVGSC--------HVRSNS------ESLSAITPYR 120

Query: 121 MSFDY-QGVLRRSQTEVFSRSSE---------------KNGGGVWKVKLVISPKRLVEIL 180
           MS DY   VL+RS T+VFSR+S                 + G +WKV L+I+ + L++IL
Sbjct: 121 MSLDYNHRVLKRSYTDVFSRNSHIRTRQKEKKTRRRRTSSKGAIWKVNLIINTEELLQIL 180

Query: 181 EEEGHTQELIESVRTVAKCGSTSTSSSFSS 194
            E+G T ELIESVR VAK G TS+ +S SS
Sbjct: 181 SEDGRTNELIESVRAVAK-GETSSITSSSS 194

[Close]

BLAST of CmoCh16G006580.1 vs. TAIR 10
Match: AT3G61920.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; EXPRESSED IN: shoot, hypocotyl, root, egg cell; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64700.1); Has 77 Blast hits to 77 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 77; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-23
Identity = 87/222 (39.19%), Postives = 110/222 (49.55%), Query Frame = 0


Query: 1   MGNCLF--GGGSGEIQGK----IKVITSNGGIMELGSPITVGCIADEFPGYGIFKSQDLF 60
           MGNC+F   GGS ++  K    IKV+T NGG+MEL  PI    I +EFPG+ I  S  L 
Sbjct: 1   MGNCVFKGNGGSRKLYDKDDSLIKVVTPNGGVMELHPPIFAEFITNEFPGHVIHDSLSLR 60

Query: 61  WN--PLPHNEELLPGKSYYLLQRNRGRNRGETEEGEMGMIRAREGHVRSNSVPEATAAAA 120
            +  PL H EEL PG  YYLL                               P +++AAA
Sbjct: 61  HSSPPLLHGEELFPGNIYYLL-------------------------------PLSSSAAA 120

Query: 121 AGM--------APYRMSFDYQGVLRRSQTEVFSRSSEKNGGGVWKVKLVISPKRLVEILE 180
                       PYRMSF         +T + +  S   G GVWKV+LVISP++L EIL 
Sbjct: 121 TAQLDSSDQLSTPYRMSF--------GKTPIMAALS-GGGCGVWKVRLVISPEQLAEILA 180

Query: 181 EEGHTQELIESVRTVAKCGSTSTSSSFSSSMAFSDHWSLSST 207
           E+  T+ L+ESVRTVAKCG          S A SD  S++S+
Sbjct: 181 EDVETEALVESVRTVAKCGGYGCGGGV-HSRANSDQLSVTSS 181

The following BLAST results are available for this feature:

BLAST of CmoCh16G006580.1 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh16G006580.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETY6	4.2e-119	100.00	uncharacterized protein LOC111437546 OS=Cucurbita moschata OX=3662 GN=LOC1114375...	[more]
A0A6J1JCF7	3.8e-112	97.32	uncharacterized protein LOC111483212 OS=Cucurbita maxima OX=3661 GN=LOC111483212...	[more]
A0A0A0KUU6	4.2e-103	91.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047890 PE=4 SV=1	[more]
A0A5D3D1S5	5.5e-103	90.63	DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BSZ8	5.5e-103	90.63	uncharacterized protein LOC103493172 OS=Cucumis melo OX=3656 GN=LOC103493172 PE=...	[more]

BLAST of CmoCh16G006580.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64700.1	5.9e-33	43.81	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin...	[more]
AT3G61920.1	1.9e-23	39.19	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025322	Protein of unknown function DUF4228, plant	PFAM	PF14009	DUF4228	coord: 1..171 e-value: 5.2E-17 score: 62.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..224
None	No IPR available	PANTHER	PTHR33148:SF48	DUF4228 DOMAIN PROTEIN	coord: 1..208
None	No IPR available	PANTHER	PTHR33148	PLASTID MOVEMENT IMPAIRED PROTEIN-RELATED	coord: 1..208

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh16G006580	CmoCh16G006580	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G006580.1:exon:10948	CmoCh16G006580.1:exon:10948	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G006580.1:cds	CmoCh16G006580.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh16G006580.1	CmoCh16G006580.1-protein	polypeptide