CmoCh08G004820.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G004820.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	protein NIM1-INTERACTING 1
Location	Cmo_Chr08: 2979922 .. 2980317 (-)
Sequence length	396
RNA-Seq Expression	CmoCh08G004820.1
Synteny	CmoCh08G004820.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGCCGCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCGACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

mRNA sequence

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGCCGCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCGACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

Coding sequence (CDS)

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGCCGCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCGACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

Protein sequence

MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEEDILDLNLSL

Homology

[Close]

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL
Match: A0A6J1H831 (protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-60
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE 60
           MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE
Sbjct: 1   MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE 60

Query: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN 120
           KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN
Sbjct: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN 120

Query: 121 EEDILDLNLSL 132
           EEDILDLNLSL
Sbjct: 121 EEDILDLNLSL 131

[Close]

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL
Match: A0A6J1KR57 (protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.2e-47
Identity = 116/133 (87.22%), Postives = 117/133 (87.97%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDGEE--EEEMKMEAFFALVRNFKEMRDQRRKELMIGCD 60
           MEKQSLKTTGKRV    AAA VD EE  EEEMKMEAFF LVRNFKEMRDQRRKELMI CD
Sbjct: 1   MEKQSLKTTGKRV----AAATVDREEEKEEEMKMEAFFDLVRNFKEMRDQRRKELMIVCD 60

Query: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120
           GEKAAAAEMRK+KMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA      KKKKV
Sbjct: 61  GEKAAAAEMRKKKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA------KKKKV 120

Query: 121 KNEEDILDLNLSL 132
           KNEEDILDLNLSL
Sbjct: 121 KNEEDILDLNLSL 123

[Close]

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL
Match: A0A6J1DAZ5 (protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.7e-19
Identity = 69/120 (57.50%), Postives = 81/120 (67.50%), Query Frame = 0


Query: 25  EEEEEMKMEAFFALVRNFKEMRDQRRKEL--MIGCDGEKAAAAEMRKRKMKTVENRSAWI 84
           EEEEEMKMEAFFAL+RNFKEMRD+RRKEL  MIG  GE     E +++KMKT    S WI
Sbjct: 13  EEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGE----TEKKRKKMKTAP--STWI 72

Query: 85  PRFEREDFDEEFQVRGP----------AKEEAAAAAI-ATAKKKKKVKNEEDILDLNLSL 132
           P F REDFDE+FQVRGP           KE+     + A   KKK +KN+E+ LDLNL+L
Sbjct: 73  PTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPLPAAVAKKKNMKNDEECLDLNLAL 126

[Close]

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL
Match: A0A6J1JVD5 (uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.1e-17
Identity = 64/118 (54.24%), Postives = 82/118 (69.49%), Query Frame = 0


Query: 25  EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGEKAAAAEMRKR--KMKTVENRSAWI 84
           EEEEE++ME F+ALVRNFKE+RD+ RKE++ G +G++ AA + RKR    +TV+N+S+WI
Sbjct: 16  EEEEEVEMEKFYALVRNFKEIRDRWRKEVVNG-EGDETAAEKTRKRMKTTETVQNQSSWI 75

Query: 85  PRFEREDFDEEFQVRGPAKE---------EAAAAAIATAKKKKKVKNEEDILDLNLSL 132
            RFEREDFDE+ QV G A E         E    A A  KK+KKV +  D LDLNLSL
Sbjct: 76  LRFEREDFDEDLQVGGAAVELPKHCCLVKEDTPVAAAPLKKEKKVASGVDCLDLNLSL 132

[Close]

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL
Match: A0A0A0K5V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 7.8e-17
Identity = 68/128 (53.12%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 21  AVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCD--GEKAAAAEMRKRKMKT---V 80
           A D  E+EE  ME FFALVR+ KEMRDQRRKEL IG +   E+ AA E R+++M+    V
Sbjct: 14  AEDSLEDEE--MERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEKRRKRMRAAEMV 73

Query: 81  ENRSAWIPRFEREDFDEEFQVRGP---------AKEEAAAAAIATA---KKKKKVKNEED 132
           ENRS WIP+FEREDFDEEFQ              K +  AA+  T    K KKKVKN + 
Sbjct: 74  ENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPMKNKKKVKNGDV 133

[Close]

BLAST of CmoCh08G004820.1 vs. NCBI nr
Match: XP_022960173.1 (protein NIM1-INTERACTING 1 [Cucurbita moschata])

HSP 1 Score: 241.1 bits (614), Expect = 5.3e-60
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE 60
           MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE
Sbjct: 1   MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE 60

Query: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN 120
           KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN
Sbjct: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN 120

Query: 121 EEDILDLNLSL 132
           EEDILDLNLSL
Sbjct: 121 EEDILDLNLSL 131

[Close]

BLAST of CmoCh08G004820.1 vs. NCBI nr
Match: KAG6593261.1 (hypothetical protein SDJN03_12737, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 226.9 bits (577), Expect = 1.0e-55
Identity = 125/131 (95.42%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGE 60
           MEKQSLKTTGKRVAS    AAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC+GE
Sbjct: 1   MEKQSLKTTGKRVAS----AAVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGE 60

Query: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKN 120
           KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAI TAKKKKKVKN
Sbjct: 61  KAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIVTAKKKKKVKN 120

Query: 121 EEDILDLNLSL 132
           EEDILDLNLSL
Sbjct: 121 EEDILDLNLSL 127

[Close]

BLAST of CmoCh08G004820.1 vs. NCBI nr
Match: KAG7025612.1 (hypothetical protein SDJN02_12109, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.4 bits (568), Expect = 1.1e-54
Identity = 126/133 (94.74%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDG--EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCD 60
           MEKQSLKTTGKRVAS    AAVDG  EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC+
Sbjct: 1   MEKQSLKTTGKRVAS----AAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCN 60

Query: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120
           GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV
Sbjct: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120

Query: 121 KNEEDILDLNLSL 132
           KNEEDILDLNLSL
Sbjct: 121 KNEEDILDLNLSL 129

[Close]

BLAST of CmoCh08G004820.1 vs. NCBI nr
Match: XP_023514605.1 (protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 208.0 bits (528), Expect = 5.0e-50
Identity = 120/132 (90.91%), Postives = 121/132 (91.67%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDG-EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDG 60
           MEKQSLK TGKRV    A AAVDG EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDG
Sbjct: 1   MEKQSLKPTGKRV----AVAAVDGEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDG 60

Query: 61  EKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVK 120
           EKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQ+RGPAKEEAAAA    AKKKKKVK
Sbjct: 61  EKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQLRGPAKEEAAAA----AKKKKKVK 120

Query: 121 NEEDILDLNLSL 132
           NEEDILDLNLSL
Sbjct: 121 NEEDILDLNLSL 124

[Close]

BLAST of CmoCh08G004820.1 vs. NCBI nr
Match: XP_023004697.1 (protein NIM1-INTERACTING 1 [Cucurbita maxima])

HSP 1 Score: 197.2 bits (500), Expect = 8.8e-47
Identity = 116/133 (87.22%), Postives = 117/133 (87.97%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAAAAAVDGEE--EEEMKMEAFFALVRNFKEMRDQRRKELMIGCD 60
           MEKQSLKTTGKRV    AAA VD EE  EEEMKMEAFF LVRNFKEMRDQRRKELMI CD
Sbjct: 1   MEKQSLKTTGKRV----AAATVDREEEKEEEMKMEAFFDLVRNFKEMRDQRRKELMIVCD 60

Query: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120
           GEKAAAAEMRK+KMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA      KKKKV
Sbjct: 61  GEKAAAAEMRKKKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA------KKKKV 120

Query: 121 KNEEDILDLNLSL 132
           KNEEDILDLNLSL
Sbjct: 121 KNEEDILDLNLSL 123

[Close]

BLAST of CmoCh08G004820.1 vs. TAIR 10
Match: AT1G02450.1 (NIM1-interacting 1 )

HSP 1 Score: 42.0 bits (97), Expect = 4.4e-04
Identity = 34/114 (29.82%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 23  DGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGEKAAAAEMRKRKMKTVENRSAWI 82
           D EEEEE K++ FF L+++++E R +RR+EL        A  + + +RK    E     +
Sbjct: 37  DEEEEEEKKIDTFFKLIKHYQEARKRRREEL--------AENSGVVRRKSNGGERSGIVV 96

Query: 83  PRFEREDFDE-EFQVRGP----AKEEAAAAAIATAKKKKKVKNEEDILDLNLSL 132
           P F+ EDF +    ++ P    +  +     +   + + + +NE+  LDLNL+L
Sbjct: 97  PAFQPEDFSQCRTGLKPPLMFVSDHKEENTKVEQEEDQTEERNEDKALDLNLAL 142

The following BLAST results are available for this feature:

BLAST of CmoCh08G004820.1 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh08G004820.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H831	2.6e-60	100.00	protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV...	[more]
A0A6J1KR57	4.2e-47	87.22	protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1	[more]
A0A6J1DAZ5	1.7e-19	57.50	protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 S...	[more]
A0A6J1JVD5	2.1e-17	54.24	uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189...	[more]
A0A0A0K5V7	7.8e-17	53.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1	[more]

BLAST of CmoCh08G004820.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960173.1	5.3e-60	100.00	protein NIM1-INTERACTING 1 [Cucurbita moschata]	[more]
KAG6593261.1	1.0e-55	95.42	hypothetical protein SDJN03_12737, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7025612.1	1.1e-54	94.74	hypothetical protein SDJN02_12109, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023514605.1	5.0e-50	90.91	protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo]	[more]
XP_023004697.1	8.8e-47	87.22	protein NIM1-INTERACTING 1 [Cucurbita maxima]	[more]

BLAST of CmoCh08G004820.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02450.1	4.4e-04	29.82	NIM1-interacting 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031425	NPR1/NH1-interacting protein	PFAM	PF15699	NPR1_interact	coord: 25..127 e-value: 4.3E-18 score: 66.0
IPR031425	NPR1/NH1-interacting protein	PANTHER	PTHR33669	PROTEIN NEGATIVE REGULATOR OF RESISTANCE	coord: 17..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR33669:SF1	NPR1/NH1-INTERACTING PROTEIN-RELATED	coord: 17..131

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh08G004820	CmoCh08G004820	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh08G004820.1:exon:7998	CmoCh08G004820.1:exon:7998	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh08G004820.1:cds	CmoCh08G004820.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh08G004820.1	CmoCh08G004820.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010112	regulation of systemic acquired resistance
cellular_component	GO:0005634	nucleus