CmoCh08G001250.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G001250.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionWRKY protein
LocationCmo_Chr08: 642446 .. 647644 (-)
Sequence length1562
RNA-Seq ExpressionCmoCh08G001250.1
SyntenyCmoCh08G001250.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTAGAGAGAGAGATGAACAATTTGAAGGCATTGAATTCCATTGATGTCTTTGTTTTACTCGTTCCCCAATCTTTTCCATGTTTTTTCCTTTCAGTTTCTCCAGTCACAATCATGGAGTTTTTTTTTTTCTCTCTCTCTCTCTCTCTTTGTTCCCCCTTCCTTTATAAGCCATTTTCATGCTTCCTCTGCTTCCCCAGCTTCGATCATGGAGTTTGTGAATCTAATCCAAGAGCTTAATCAGGGGAGAAACCTCGCTCACCAATTACGCGATCATCTTCATCCTTCTCTTTCTTCTTCTTCTTCTTCTCCGTCTTCAAATGGCATTCTCTTGATCGATCAGATTCTTCGTTCTTACGAGAACGCGCTTTCTGTTCTCGCCGGCGGAAGCAGCGGCGGCGATGAAGAAATCCCGCCCACCATTGCGGCTGTGAAACACAGAGACGTATCGAAGAAGAGGTAATTTCGTAATCGATTGTTTTGGGATTTTAGGGTTTTGTTGGTTCGTGATTCTGATTGGATTTTAGGGTTTTGTGTTTTGCAGAAAGGTAATGGCGAAATGGAGTGAGCTGGTTAAGGTTCCTTCTGTCTCCACCATTGGAGCTCTTCCTGATGATGGATTTAGCTGGAGAAAGTATGGGCAGAAGGACATTCTTGGATCCAAATTCCCCAGGTCGCTTACCTTTTTTTCTCATTATGTTCTCTTCTCTGTTTCAAATCGTGTTCATATTGTTCCTCTGTTCTTGTTCCTGCGGGTTTAATCATCTAAACTGAAAAGAGTTTTTGAGAATCGTTGAGAGAGGAGTCTCACATCGATCATCAATTTATAGATAAGGAATACGTCTTCATTGGTATGCCATTTTGAAAACCATGAGAGCTTATGCTCGAAATGTACAATATCATATCACTGTGGAGGGTTGTTGTTCGATTTAATGGATGAAAACAATGATTATGATCAATTCAGTCGATCACATCAACGATTTATGAAGTAATATGACAGCAAAACTGAATCATTTTGTTGTGTTTTGGGAATTGCAGAGGCTATTTCAGATGCTCACACCGCTTTGCACAAGGATGTTCAGCGACAAAACAAGTTCAAAGATTAGACAATGATCCATCCATGTATGAAATAACGTACAGAGGTAAACACACCTGCAACAAACCTCTTCATTCAAACACACCAAAACAAGAACAAACCCCACAATTACAGCAACATCCAATGCTAATGCCACCAAAACAAGAACAAGAGGAGCCATTGCAGCAGCAGGATGCCCCATTGTACCTCACCGTCAGCTCGGACAATGTGGACGACAGCCTCCGACGACCCTTCTGTCCCCCGTCGCCATTGTTTCGATCTGAAGTTGAAGATGATCAAAGCCCTTTTAGGGGAAGTGAGTTGGAGGGGTTAGAATTTTGGTGTATTGGGGATGGAGATTTGGTCTCAGGTCCGAGTTCATTCACCAATATTTCCATGGGAGATTTGGAGGAGTATTGTAGTTTGGAATCCTTCTGCTGAATATAAACAAGCAATCGGCATTGAAAGAGAGATGATTTGATAATGTGAAGGTCTCCTGCATTGCTGCTTGGTTTTGAATTCAGTGTACTTATTCATCTTTCCGTAGGAGAAAATAGGCATTTGGTAACTTTCTTACCACTTGTGTGTTTGAATTAGAACAATAAGATTGGAATTCGTATTCATATTTTTTTCTAAAAGGACCAAAATACCCTCATTTTCAAGTCCCGAGCCGAGATGTAATCCATCGTTGAAGGGAGTTAACGATGTAACGAAGCTTGTGTCTTAAAAGATTCAATCCTCAATCCAAGGAGGCGTTTTAGAAGTCGGGTCCTCTCACAAAACCAATGCACATAGCTCGAGGAAGAGGAGATGTGAGATCCCACATTAGTTAGAGAGGGGAATAAAACATTCCTTAAAACCTCTCCCTAGTAGACACACTTTAAAACTGTGATGCTGACGACGATACGTAACGAGCCAATGGACAATACCTGCTAGCGGTGGGCTTACAAATGTTATCAGAGTCATTCATTGGGGCGGTGTGTCAACGTGGACATTGGGTCCTCAAGGAGAGAGGGGATGGATTGTGAGATCCCACATCGATTAGATAGGAGAACGAAACATTCCTTTAAAACCGTGAGGCTAAAGGCGATAAGTGACGGACCAAAGCGGACAATACTTACTAGCGGTGGGCTTGAGTTGTTACAAATGTTATCAGAGTCATTCAATGGGGCGGTGTGCCAACGTGGACATTGAGCCCTCAAGGAGGGAGGGAATGGATTGTGAGATCCCACATCGAAGACAAGATAACGAAACATTCCTTTAAAACTGTGAGGTTGATGACGATAAGTAACGGGCCAAAGTGGACAATACCTACTAGCGGTGGACTTGGGCTCTTCCAGGAGAAAAGTTTTTTGTGGTTGGTGGCATCATCGGCTTTGTGGGTAACACATGTCAACTAAGAATATGTACAAAGTATTCTCGTTATCTTTAATTCATCAATTATGTATTTTTTCGAATAATTTGATTAGCATTAACGATAAACGTTTAAAATATTTTATCAACAAAAATAAACTTACAAAAATTACTGTCAAAATTTTCTCGCTTTTGGGTAATAAATATTTACTCACAGTAGTTTTGTTTTCAAATTCTTGTAACTAATAAAATTTTAAACATAATTTGCAAATATCCCAAAGTATTCTCGATTTGTTCATAAGGGCCTCGAACCAATGACAATCTCACCGTCCATTTGGTCAGATCTGAACCACCAAAGTTTTGGGCCTGAAAATGGTAAGCCCATTTAGTGAGTTCCAGCCTATAAGCACGATAGCTGATGCTCACCATTTGAGCCATTTTGGTCTTTGGGGAATCAGACGGAAAAAGGAGCAAAACCAAAGATGATCTACCGGAAATGGAGTCTTCTCACCGGCCCCACCGCTATCTTGGGCGGCATAGTTGTCTCTCTGGCCGTCGCAAACTTCATCTTCGTTAAAGAAGTAATCCCTTGCTTTCCTTAACTCCCTTCGCTTTTGATTTTTAATTCATCTAGATTCTTTCCAATATTTTGGTGGTGTTGTTATTAATTTGTGTGTATGTGTGTTCTTGTATTGATCTGTTTGCCATGATTTGGATTTGTTGTTGTGCGGCCGACTAGATTTAGGGTTTCGCATAATTTTTCCTTCTGCTCTCTTTTGGTATCTGGAAATCGTGTTATGAAATTGAAAATGAATCTGTTCGGTGGAGATGAATGTGGCGTTGTGGCGTTTTTCCTTATTTTCTTTTGAAACTTTCGGTTTTGCCTGACAAATTTGGAATCTAGGAACATTTCACGCATTTTTCTAATCTCGGGACTTGAATTTGGTAGGATGAATGCTTTACTCTGGGGTTGGGTGATCATTTAGTCCAAGAGTTTAAGCAGTTGAAGCAAGATTATGTTAATATTTTCGATATCTCTATTTGGTTAAGTTGAATTTTTTTCTTTTCAGTATATTATTAAAACTTCATTCCATAATTACTGTATGTGCCTGTGGGTAGGCAACTGGTTCATACTTTTCCACTATATTCATTTACAGACAATAGAATTGCTGCATGTCTCTGAGTTGCCCCTTTTAATTATGTCTATATATTGTATTATTGGCCATTGATGTGTTGGCAATTCTCACTCATGGTTTGAGCTCTCTTAATGTTGCCGAAACTTCAATAAATCTAAAGCTTAAGTGACTTCAATCTTAACTTGTCGGTGTCTTTATAAATGAAGTTTGTACTTGAGTAGTACTTTCATTTAGCCTGGCTTTTCCATTTAGTGTTTTGACGATGAATAGATCAAAATAGTAACACCATATTTTTTTTCTTCCTTTAATGAATCGACCTGAGGATACAAAACAGTTGGTGGTAAGGCTTGTAGATGGACCTCCCCATTGTACTCTTTCTTTTTCTTTAGCAATTCCATAAGAGGGGGCTGATAAGGCTGCTGATAAAGCACAAGCCAAACACCTCATAATGTACCTAAGCATGGCTTGGCCTCTTTTGCTGAGGTTTGCTCTGGCATATCAGGCACGGCTTCAATTTTTAGTTGAGTCCACATGTGTGCCTTGTCAACTCACCAAATTGTTAGACGAACACGACTCTCCACAATGGTATGGTATTGTCCACTTTGAGCATTAAGCTCTCATGGCTTTGCTTTGGGATTCCCCAAAAGGCCTCATACCAATGGAGAGAGCATTCCTTGATTATAAACCCATGATCATTCCCTAAATTAGCGGATGTGAGACTTTCATCATCCAACACAGATAACCAAGACATTTCTTTGAATGTTATGTTGAAGGTGCGCTGTGGTATTACGCCAAATTTACATCTTCAACCGAGAAATTCTTGATCATTCCAAAAACCCTTTGTAGAGCTTCTACGGGCCATTCTTATATCTTGGATTTGAAAATATATTATCTGTTCGTAGGTAGTTCTTACAGTTACAGCTTCTAATTAGCAATTAATTAACCATCTATTGGGGATCATTATTATGGATTCTGGATCAAGTATGAGTTTCCTTCTAGATACAATTTCTTGTAATATAGAAATTTGTCCAGAAAATTTATGTTGAATTCAAATGTTTATTTATATTGCTAACCATTAAAAATTCCAAGATATTGGGCAGGAATCTTCACAAAGGAAAGAAGAAAACTCTTTGGAACACTGTTATTAAGTCTCTCATTGATTGGCCAGGAAGAAATAAGAAAATTTGTAAAATTCACTCGAACAATGTTATTAGGGTCTTTCATTGAAAACTTAACGTCAAAGAAAACTGATAAATTTTCCGATAGTTTTAATCATATTTGTATCTTTTGGAAATGGGCATGCCACAAAATGGAGCTCGCTTTGTAAAACCTTTTGTAATGAGAATTTGGTGCCAATTGGAGTGTTTCTTCCTCATCCTGTGTTTTGTATTTCATTTTTCGATGAGTGGTGTTTATTTCTCAAAATAAAAACATAATTGATACTTCTTTTTTCCTTAATTGACCGTAATCATTCCTATGAATGTTCATTTGCAGGATCCATTTCTGAAACCAAAGGAAAGGAAGCCGGAACCAGCTACAAAATGAAGGATATATTCAGCGAAGGTTCCAATTTAAATCATCTAGAGATTGTATCTGGATTGGTGTTGTCTTTAAATTGTTGTTTTGCTTTCTCATGA

mRNA sequence

GGTAGAGAGAGAGATGAACAATTTGAAGGCATTGAATTCCATTGATGTCTTTGTTTTACTCGTTCCCCAATCTTTTCCATGTTTTTTCCTTTCAGTTTCTCCAGTCACAATCATGGAGTTTTTTTTTTTCTCTCTCTCTCTCTCTCTTTGTTCCCCCTTCCTTTATAAGCCATTTTCATGCTTCCTCTGCTTCCCCAGCTTCGATCATGGAGTTTGTGAATCTAATCCAAGAGCTTAATCAGGGGAGAAACCTCGCTCACCAATTACGCGATCATCTTCATCCTTCTCTTTCTTCTTCTTCTTCTTCTCCGTCTTCAAATGGCATTCTCTTGATCGATCAGATTCTTCGTTCTTACGAGAACGCGCTTTCTGTTCTCGCCGGCGGAAGCAGCGGCGGCGATGAAGAAATCCCGCCCACCATTGCGGCTGTGAAACACAGAGACGTATCGAAGAAGAGAAAGGTAATGGCGAAATGGAGTGAGCTGGTTAAGGTTCCTTCTGTCTCCACCATTGGAGCTCTTCCTGATGATGGATTTAGCTGGAGAAAGTATGGGCAGAAGGACATTCTTGGATCCAAATTCCCCAGAGGCTATTTCAGATGCTCACACCGCTTTGCACAAGGATGTTCAGCGACAAAACAAGTTCAAAGATTAGACAATGATCCATCCATGTATGAAATAACGTACAGAGGTAAACACACCTGCAACAAACCTCTTCATTCAAACACACCAAAACAAGAACAAACCCCACAATTACAGCAACATCCAATGCTAATGCCACCAAAACAAGAACAAGAGGAGCCATTGCAGCAGCAGGATGCCCCATTGTACCTCACCGTCAGCTCGGACAATGTGGACGACAGCCTCCGACGACCCTTCTGTCCCCCGTCGCCATTGTTTCGATCTGAAGTTGAAGATGATCAAAGCCCTTTTAGGGGAAGTGAGTTGGAGGGGTTAGAATTTTGGTGTATTGGGGATGGAGATTTGGTCTCAGGTCCGAGTTCATTCACCAATATTTCCATGGGAGATTTGGAGGAGTCTCCTGCATTGCTGCTTGGTTTTGAATTCAGTGTACTTATTCATCTTTCCGTAGGAGAAAATAGGCATTTGACGGAAAAAGGAGCAAAACCAAAGATGATCTACCGGAAATGGAGTCTTCTCACCGGCCCCACCGCTATCTTGGGCGGCATAGTTGTCTCTCTGGCCGTCGCAAACTTCATCTTCGTTAAAGAAGGGGCTGATAAGGCTGCTGATAAAGCACAAGCCAAACACCTCATAATGTACCTAAGCATGGCTTGGCCTCTTTTGCTGAGGTTTGCTCTGGCATATCAGGCACGGCTTCAATTTTTAGTTGAGTCCACATGTGTGCCTTGTCAACTCACCAAATTGTTAGACGAACACGACTCTCCACAATGGTATGGATCCATTTCTGAAACCAAAGGAAAGGAAGCCGGAACCAGCTACAAAATGAAGGATATATTCAGCGAAGGTTCCAATTTAAATCATCTAGAGATTGTATCTGGATTGGTGTTGTCTTTAAATTGTTGTTTTGCTTTCTCATGA

Coding sequence (CDS)

ATGTCTTTGTTTTACTCGTTCCCCAATCTTTTCCATGTTTTTTCCTTTCAGTTTCTCCAGTCACAATCATGGAGTTTTTTTTTTTCTCTCTCTCTCTCTCTCTTTGTTCCCCCTTCCTTTATAAGCCATTTTCATGCTTCCTCTGCTTCCCCAGCTTCGATCATGGAGTTTGTGAATCTAATCCAAGAGCTTAATCAGGGGAGAAACCTCGCTCACCAATTACGCGATCATCTTCATCCTTCTCTTTCTTCTTCTTCTTCTTCTCCGTCTTCAAATGGCATTCTCTTGATCGATCAGATTCTTCGTTCTTACGAGAACGCGCTTTCTGTTCTCGCCGGCGGAAGCAGCGGCGGCGATGAAGAAATCCCGCCCACCATTGCGGCTGTGAAACACAGAGACGTATCGAAGAAGAGAAAGGTAATGGCGAAATGGAGTGAGCTGGTTAAGGTTCCTTCTGTCTCCACCATTGGAGCTCTTCCTGATGATGGATTTAGCTGGAGAAAGTATGGGCAGAAGGACATTCTTGGATCCAAATTCCCCAGAGGCTATTTCAGATGCTCACACCGCTTTGCACAAGGATGTTCAGCGACAAAACAAGTTCAAAGATTAGACAATGATCCATCCATGTATGAAATAACGTACAGAGGTAAACACACCTGCAACAAACCTCTTCATTCAAACACACCAAAACAAGAACAAACCCCACAATTACAGCAACATCCAATGCTAATGCCACCAAAACAAGAACAAGAGGAGCCATTGCAGCAGCAGGATGCCCCATTGTACCTCACCGTCAGCTCGGACAATGTGGACGACAGCCTCCGACGACCCTTCTGTCCCCCGTCGCCATTGTTTCGATCTGAAGTTGAAGATGATCAAAGCCCTTTTAGGGGAAGTGAGTTGGAGGGGTTAGAATTTTGGTGTATTGGGGATGGAGATTTGGTCTCAGGTCCGAGTTCATTCACCAATATTTCCATGGGAGATTTGGAGGAGTCTCCTGCATTGCTGCTTGGTTTTGAATTCAGTGTACTTATTCATCTTTCCGTAGGAGAAAATAGGCATTTGACGGAAAAAGGAGCAAAACCAAAGATGATCTACCGGAAATGGAGTCTTCTCACCGGCCCCACCGCTATCTTGGGCGGCATAGTTGTCTCTCTGGCCGTCGCAAACTTCATCTTCGTTAAAGAAGGGGCTGATAAGGCTGCTGATAAAGCACAAGCCAAACACCTCATAATGTACCTAAGCATGGCTTGGCCTCTTTTGCTGAGGTTTGCTCTGGCATATCAGGCACGGCTTCAATTTTTAGTTGAGTCCACATGTGTGCCTTGTCAACTCACCAAATTGTTAGACGAACACGACTCTCCACAATGGTATGGATCCATTTCTGAAACCAAAGGAAAGGAAGCCGGAACCAGCTACAAAATGAAGGATATATTCAGCGAAGGTTCCAATTTAAATCATCTAGAGATTGTATCTGGATTGGTGTTGTCTTTAAATTGTTGTTTTGCTTTCTCATGA

Protein sequence

MSLFYSFPNLFHVFSFQFLQSQSWSFFFSLSLSLFVPPSFISHFHASSASPASIMEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGGSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQTPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEESPALLLGFEFSVLIHLSVGENRHLTEKGAKPKMIYRKWSLLTGPTAILGGIVVSLAVANFIFVKEGADKAADKAQAKHLIMYLSMAWPLLLRFALAYQARLQFLVESTCVPCQLTKLLDEHDSPQWYGSISETKGKEAGTSYKMKDIFSEGSNLNHLEIVSGLVLSLNCCFAFS
Homology
BLAST of CmoCh08G001250.1 vs. ExPASy Swiss-Prot
Match: Q9SUP6 (Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.5e-30
Identity = 96/269 (35.69%), Postives = 136/269 (50.56%), Query Frame = 0

Query: 60  LIQELNQGRNLAHQLRDHLH--PSLSSSSSSPSS----NGILLIDQILRSYENALSVLAG 119
           L+ EL  G + A +L+  L   PS SSS SSP++       +L+ QI+ SYE +L +L  
Sbjct: 14  LLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSLLLLNW 73

Query: 120 GSSGGDEEIPPTIAAV---------------------------------KHRD----VSK 179
            SS   + IP  +  V                                  HR      SK
Sbjct: 74  SSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDYIFNSK 133

Query: 180 KRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGCSA 239
           KRK++ KWSE V++     +    DD FSWRKYGQKDILG+KFPR Y+RC+HR  Q C A
Sbjct: 134 KRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHRSTQNCWA 193

Query: 240 TKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQTPQLQQHPMLMPPKQEQEEPLQQ 286
           TKQVQR D D +++E+TYRG HTC++ +    P    +P+ +Q   + P   ++ + +  
Sbjct: 194 TKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPL--ASPEKRQDTRVKPAITQKPKDI-L 253

BLAST of CmoCh08G001250.1 vs. ExPASy Swiss-Prot
Match: Q9SKD9 (Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.6e-27
Identity = 75/169 (44.38%), Postives = 100/169 (59.17%), Query Frame = 0

Query: 54  IMEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAG 113
           +ME   +I EL  G+ LA++L ++L       +SS  SN  L+ D ILR Y+NA+ +L+ 
Sbjct: 2   MMEEKLVINELELGKELANRLMNNL-----KHTSSVDSNKTLISD-ILRIYQNAIFMLSF 61

Query: 114 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKV-PSVSTIGALPDDGFSWRKYGQK 173
                  +    I     ++V KKRKV  K +E VKV  +        DDG  WRKYGQK
Sbjct: 62  NQDKNILKRSLEIDGKDSKNVFKKRKVSEKNTEKVKVFVATEQENGSIDDGHCWRKYGQK 121

Query: 174 DILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCN 222
           +I GSK PR Y+RC+HRF Q C A KQVQ+ D DPS++E+ Y G HTCN
Sbjct: 122 EIHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTCN 164

BLAST of CmoCh08G001250.1 vs. ExPASy Swiss-Prot
Match: Q8H0Y8 (Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 1.1e-26
Identity = 102/323 (31.58%), Postives = 155/323 (47.99%), Query Frame = 0

Query: 59  NLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSN-GILLIDQILRSYENALSVLAGGSSG 118
           +L+ EL  G   A QL+    PSLS+SSS  ++     L+  I+ S++ A+ +L G ++ 
Sbjct: 10  SLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNGSTTQ 69

Query: 119 GDEEI----------------PPTIAA---------VKHRDV---SKKRKVMAKWSELVK 178
            +  I                P +I           V+ ++    SKKRK++ KW+E V+
Sbjct: 70  HNPTIELAPDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTEQVR 129

Query: 179 VPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSM 238
           +     +    DD FSWRKYGQKDILG+KFPR Y+RC+ R  Q C ATKQVQR D DP++
Sbjct: 130 ISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPTI 189

Query: 239 YEITYRGKHTCNKPLHSNTPKQEQTPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDN 298
           +E+TYRG HTC++ +    P++ +T            K +    +  Q+    LTV +  
Sbjct: 190 FEVTYRGTHTCSQGI--PLPEKRET------------KPKHTVAVNYQNLRASLTVRTGG 249

Query: 299 VDDSLRRPFCPPSPLFRSEVEDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDL 351
           +  S    F   SPL+  E  +    F         +  +G     SGPS FT +   + 
Sbjct: 250 L-GSEAFSFPVTSPLYTYESINGGGTF---------YHHVGS----SGPSDFTGLISTNT 304

BLAST of CmoCh08G001250.1 vs. ExPASy Swiss-Prot
Match: Q9FL62 (Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-26
Identity = 73/190 (38.42%), Postives = 106/190 (55.79%), Query Frame = 0

Query: 65  NQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVL-----------AG 124
           N+   L  + RD+ H   S+SS     +   L  +IL+SY  +L+++            G
Sbjct: 15  NEINELMIEGRDYAHQFGSASSQETREH---LAKKILQSYHKSLTIMNYSGELDQVSQGG 74

Query: 125 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKD 184
           GS   D+     +       +   +K M +WS  V++   + +    DDGFSWRKYGQKD
Sbjct: 75  GSPKSDDSDQEPLV------IKSSKKSMPRWSSKVRIAPGAGVDRTLDDGFSWRKYGQKD 134

Query: 185 ILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNT--PKQ 241
           ILG+KFPRGY+RC++R +QGC ATKQVQR D +  + EI+YRG H+C++  +  T  P Q
Sbjct: 135 ILGAKFPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRGIHSCSQAANVGTTMPIQ 194

BLAST of CmoCh08G001250.1 vs. ExPASy Swiss-Prot
Match: Q0DFT7 (Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.8e-25
Identity = 84/224 (37.50%), Postives = 117/224 (52.23%), Query Frame = 0

Query: 58  VNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGGSSG 117
           + L  EL Q R +A +L   + P   ++          L   +  S + ++ + A  S  
Sbjct: 13  IMLATELAQLRAMARELEAKMDPDRVAARE--------LCRALASSVDRSIRLAA--SCF 72

Query: 118 GDEEIPPTIAAVKHRDVS-KKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDILG 177
              E PP  A    RD + KKRK MAK    V+V SV    +L DDG SWRKYGQKDILG
Sbjct: 73  PPPEHPPPAAGNAGRDAAFKKRKGMAKVRRQVRVTSVQDTASL-DDGLSWRKYGQKDILG 132

Query: 178 SKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNK-----PLHSNTPKQ 237
           +K+PR YFRC+HR  QGC+ATKQVQR D DP ++++ Y G HTC +        S  P+ 
Sbjct: 133 AKYPRAYFRCTHRHTQGCNATKQVQRADGDPLLFDVVYLGDHTCGQAAVAAAAQSAPPEH 192

Query: 238 EQTPQLQQHPMLMPPKQ--EQEEPLQQQDAP-LYLTVSSDNVDD 273
               Q +Q  +L    +   Q+   +   AP L+ + ++  VDD
Sbjct: 193 AGQEQQRQSSLLAAGTEGIHQQVVAEPMAAPFLFTSTAAGGVDD 225

BLAST of CmoCh08G001250.1 vs. ExPASy TrEMBL
Match: A0A6J1H6D3 (probable WRKY transcription factor 53 OS=Cucurbita moschata OX=3662 GN=LOC111460868 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 7.4e-156
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 60

Query: 115 SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI 174
           SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI
Sbjct: 61  SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI 120

Query: 175 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT 234
           LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT
Sbjct: 121 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT 180

Query: 235 PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS 294
           PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS
Sbjct: 181 PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS 240

Query: 295 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE
Sbjct: 241 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 277

BLAST of CmoCh08G001250.1 vs. ExPASy TrEMBL
Match: A0A6J1KTV7 (probable WRKY transcription factor 53 OS=Cucurbita maxima OX=3661 GN=LOC111498163 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 1.2e-142
Identity = 263/282 (93.26%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSL----SSSSSSPSSNGILLIDQILRSYENALSV 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSL    SSSSSSPSSNGILLIDQILRSYENALSV
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSSSSSPSSNGILLIDQILRSYENALSV 60

Query: 115 LAGGSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYG 174
           LAGGS GGDEEIPP IAAVKHRDVSKKRKVMAKWSELVK+PSVS IGALPDDGFSWRKYG
Sbjct: 61  LAGGSGGGDEEIPPIIAAVKHRDVSKKRKVMAKWSELVKIPSVSAIGALPDDGFSWRKYG 120

Query: 175 QKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPK 234
           QKDILGSKFPRGYFRCSHRFAQGCSATK VQR DNDPSMYEITYRGKHTCNKPLHSNTPK
Sbjct: 121 QKDILGSKFPRGYFRCSHRFAQGCSATKLVQRSDNDPSMYEITYRGKHTCNKPLHSNTPK 180

Query: 235 QEQT-PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEV 294
           QEQ  PQ QQHPM MPPKQEQEEPLQQQD PL LTVSSDNVDDSLRRPFCP SPLFRSEV
Sbjct: 181 QEQNPPQSQQHPMPMPPKQEQEEPLQQQDVPLCLTVSSDNVDDSLRRPFCPLSPLFRSEV 240

Query: 295 EDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           ED QSPFRGSELEGLEFWCIGDGDLVS PSSFTNISMGDLEE
Sbjct: 241 EDAQSPFRGSELEGLEFWCIGDGDLVSVPSSFTNISMGDLEE 282

BLAST of CmoCh08G001250.1 vs. ExPASy TrEMBL
Match: A0A5D3C947 (Putative WRKY transcription factor 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1737G00520 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 9.2e-82
Identity = 177/295 (60.00%), Postives = 205/295 (69.49%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVL-AG 114
           MEF++LIQELNQG+ LA+QLR+HLHP       S SS+GILL+D+ILRSYENAL  L AG
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP-------SSSSHGILLVDKILRSYENALLALSAG 60

Query: 115 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKD 174
           G +  +  I P  AAVK  DVSKKRK+MAKWSE VKV S S +     DGFSWRKYGQKD
Sbjct: 61  GGASVNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPGCDGFSWRKYGQKD 120

Query: 175 ILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQ 234
           ILGS+FPRGYFRCSHRF QGC ATKQVQ+ DNDP++Y++TYRG+HTCNK LHS    QE 
Sbjct: 121 ILGSQFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEH 180

Query: 235 TPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSS----------DNVDDSLRRPFCPPSP 294
              L QHP  +PPKQE E+PLQQ   PL    SS          DN +  L +PFCPPSP
Sbjct: 181 QNSLLQHP--IPPKQE-EKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSP 240

Query: 295 LFRSEVEDDQSPFRGSEL-------EGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           +F SEV+DDQSPFR SE        +     C  +   VS PSS TNIS+GDLEE
Sbjct: 241 MFGSEVQDDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEE 285

BLAST of CmoCh08G001250.1 vs. ExPASy TrEMBL
Match: A0A1S3CGE2 (probable WRKY transcription factor 53 OS=Cucumis melo OX=3656 GN=LOC103500461 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-81
Identity = 177/295 (60.00%), Postives = 205/295 (69.49%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVL-AG 114
           MEF++LIQELNQG+ LA+QLR+HLHP       S SS+GILL+D+ILRSYENAL  L AG
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP-------SSSSHGILLVDKILRSYENALLALSAG 60

Query: 115 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKD 174
           G +  +  I P  AAVK  DVSKKRK+MAKWSE VKV S S +     DGFSWRKYGQKD
Sbjct: 61  GGASVNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPCCDGFSWRKYGQKD 120

Query: 175 ILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQ 234
           ILGS+FPRGYFRCSHRF QGC ATKQVQ+ DNDP++Y++TYRG+HTCNK LHS    QE 
Sbjct: 121 ILGSQFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEH 180

Query: 235 TPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSS----------DNVDDSLRRPFCPPSP 294
              L QHP  +PPKQE E+PLQQ   PL    SS          DN +  L +PFCPPSP
Sbjct: 181 QNSLLQHP--IPPKQE-EKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSP 240

Query: 295 LFRSEVEDDQSPFRGSEL-------EGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           +F SEV+DDQSPFR SE        +     C  +   VS PSS TNIS+GDLEE
Sbjct: 241 MFGSEVQDDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEE 285

BLAST of CmoCh08G001250.1 vs. ExPASy TrEMBL
Match: E7CEY4 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 4.7e-78
Identity = 171/295 (57.97%), Postives = 201/295 (68.14%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 114
           MEF++LIQELNQG+ LA+QLR HLHP       S SS+GILLID+ILRSYENAL  L+GG
Sbjct: 1   MEFLSLIQELNQGKQLANQLRTHLHP-------SSSSHGILLIDKILRSYENALLALSGG 60

Query: 115 SSGG-DEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKD 174
           ++   +  I P  AAV+  DVSKKRKVMAKWSE VKV S S +     DGFSWRKYGQKD
Sbjct: 61  AAASVNSAIAPVNAAVQDGDVSKKRKVMAKWSEQVKVSSASAVEGPGCDGFSWRKYGQKD 120

Query: 175 ILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQ 234
           ILGSKFPR YFRCSHRF QGC ATKQVQ+ DNDP++YE+TY+G+HTCNK LHS    QE 
Sbjct: 121 ILGSKFPRSYFRCSHRFTQGCLATKQVQKSDNDPTIYEVTYKGRHTCNKALHSTNTPQEH 180

Query: 235 TPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNV----------DDSLRRPFCPPSP 294
                QHP  +PPKQE ++PLQQ   PL    SSD +          +  L +P   PSP
Sbjct: 181 QNTFLQHP--IPPKQE-DKPLQQLHDPLCFMFSSDPIRVKSEDLEYANGGLFQPLRTPSP 240

Query: 295 LFRSEVEDDQSPFRGSEL-------EGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           +F SEV+DD SPFR SE        +    WC  + + VS PSS TNIS+GDLEE
Sbjct: 241 MFGSEVQDDLSPFRESECSPTFESNDMFGLWCDFETEFVSIPSSMTNISIGDLEE 285

BLAST of CmoCh08G001250.1 vs. NCBI nr
Match: XP_022959976.1 (probable WRKY transcription factor 53 [Cucurbita moschata])

HSP 1 Score: 560.5 bits (1443), Expect = 1.5e-155
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 60

Query: 115 SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI 174
           SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI
Sbjct: 61  SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI 120

Query: 175 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT 234
           LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT
Sbjct: 121 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT 180

Query: 235 PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS 294
           PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS
Sbjct: 181 PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS 240

Query: 295 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE
Sbjct: 241 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 277

BLAST of CmoCh08G001250.1 vs. NCBI nr
Match: KAG7025317.1 (putative WRKY transcription factor 46, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.0 bits (1390), Expect = 2.1e-149
Identity = 270/277 (97.47%), Postives = 270/277 (97.47%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAGG 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSL  SSSSPSSNGILLIDQILRSYENALSVLAGG
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSL--SSSSPSSNGILLIDQILRSYENALSVLAGG 60

Query: 115 SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDI 174
           SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVS IGALPDDGFSWRKYGQKDI
Sbjct: 61  SSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSAIGALPDDGFSWRKYGQKDI 120

Query: 175 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQT 234
           LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNK LHSNTPKQEQT
Sbjct: 121 LGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKSLHSNTPKQEQT 180

Query: 235 PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVEDDQS 294
           PQLQQHPM MPPKQEQEEPLQQQD PLYLTVSSDNVDDSLRRPFCP SPLFRSEVEDDQS
Sbjct: 181 PQLQQHPMPMPPKQEQEEPLQQQDVPLYLTVSSDNVDDSLRRPFCPRSPLFRSEVEDDQS 240

Query: 295 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE
Sbjct: 241 PFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 275

BLAST of CmoCh08G001250.1 vs. NCBI nr
Match: XP_023513678.1 (probable WRKY transcription factor 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.3 bits (1375), Expect = 1.2e-147
Identity = 270/281 (96.09%), Postives = 270/281 (96.09%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSL---SSSSSSPSSNGILLIDQILRSYENALSVL 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSL   SSSSSSPSSNGILLIDQILRSYENALSVL
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSSSSPSSNGILLIDQILRSYENALSVL 60

Query: 115 AGGS-SGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYG 174
           AGGS  GGDEEIPP IAAVKHRDVSKKRKVMAKWSELVKVPSVS IGALPDDGFSWRKYG
Sbjct: 61  AGGSGGGGDEEIPPAIAAVKHRDVSKKRKVMAKWSELVKVPSVSAIGALPDDGFSWRKYG 120

Query: 175 QKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPK 234
           QKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNK LHSNTPK
Sbjct: 121 QKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKSLHSNTPK 180

Query: 235 QEQTPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEVE 294
           QEQTPQLQQHPM MPPKQEQEEPLQQQD PLYLTVSSDNVDDSLRRPFCP SPLFRSEVE
Sbjct: 181 QEQTPQLQQHPMPMPPKQEQEEPLQQQDVPLYLTVSSDNVDDSLRRPFCPRSPLFRSEVE 240

Query: 295 DDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           DDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE
Sbjct: 241 DDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 281

BLAST of CmoCh08G001250.1 vs. NCBI nr
Match: KAG6592912.1 (putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531.6 bits (1368), Expect = 7.6e-147
Identity = 270/282 (95.74%), Postives = 270/282 (95.74%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSL-SSSSSSPSSNGILLIDQILRSYENALSVLAG 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSL SSSSSSPSSNGILLIDQILRSYENALSVLAG
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSSPSSNGILLIDQILRSYENALSVLAG 60

Query: 115 GSSG----GDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKY 174
           GSSG    GDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVS IGALPDDGFSWRKY
Sbjct: 61  GSSGSGGSGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSAIGALPDDGFSWRKY 120

Query: 175 GQKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTP 234
           GQKDILGSKFPRGYFRCSHRFAQGCSATKQVQR DNDPSMYEITYRGKHTCNK LHSNTP
Sbjct: 121 GQKDILGSKFPRGYFRCSHRFAQGCSATKQVQRSDNDPSMYEITYRGKHTCNKSLHSNTP 180

Query: 235 KQEQTPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEV 294
           KQEQTPQLQQHPM M PKQEQEEPLQQQD PLYLTVSSDNVDDSLRRPFCP SPLFRSEV
Sbjct: 181 KQEQTPQLQQHPMPMRPKQEQEEPLQQQDVPLYLTVSSDNVDDSLRRPFCPRSPLFRSEV 240

Query: 295 EDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           EDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE
Sbjct: 241 EDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 282

BLAST of CmoCh08G001250.1 vs. NCBI nr
Match: XP_023005056.1 (probable WRKY transcription factor 53 [Cucurbita maxima])

HSP 1 Score: 516.5 bits (1329), Expect = 2.5e-142
Identity = 263/282 (93.26%), Postives = 264/282 (93.62%), Query Frame = 0

Query: 55  MEFVNLIQELNQGRNLAHQLRDHLHPSL----SSSSSSPSSNGILLIDQILRSYENALSV 114
           MEFVNLIQELNQGRNLAHQLRDHLHPSL    SSSSSSPSSNGILLIDQILRSYENALSV
Sbjct: 1   MEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSSSSSPSSNGILLIDQILRSYENALSV 60

Query: 115 LAGGSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYG 174
           LAGGS GGDEEIPP IAAVKHRDVSKKRKVMAKWSELVK+PSVS IGALPDDGFSWRKYG
Sbjct: 61  LAGGSGGGDEEIPPIIAAVKHRDVSKKRKVMAKWSELVKIPSVSAIGALPDDGFSWRKYG 120

Query: 175 QKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPK 234
           QKDILGSKFPRGYFRCSHRFAQGCSATK VQR DNDPSMYEITYRGKHTCNKPLHSNTPK
Sbjct: 121 QKDILGSKFPRGYFRCSHRFAQGCSATKLVQRSDNDPSMYEITYRGKHTCNKPLHSNTPK 180

Query: 235 QEQT-PQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDNVDDSLRRPFCPPSPLFRSEV 294
           QEQ  PQ QQHPM MPPKQEQEEPLQQQD PL LTVSSDNVDDSLRRPFCP SPLFRSEV
Sbjct: 181 QEQNPPQSQQHPMPMPPKQEQEEPLQQQDVPLCLTVSSDNVDDSLRRPFCPLSPLFRSEV 240

Query: 295 EDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDLEE 332
           ED QSPFRGSELEGLEFWCIGDGDLVS PSSFTNISMGDLEE
Sbjct: 241 EDAQSPFRGSELEGLEFWCIGDGDLVSVPSSFTNISMGDLEE 282

BLAST of CmoCh08G001250.1 vs. TAIR 10
Match: AT4G23810.1 (WRKY family transcription factor )

HSP 1 Score: 134.4 bits (337), Expect = 2.5e-31
Identity = 96/269 (35.69%), Postives = 136/269 (50.56%), Query Frame = 0

Query: 60  LIQELNQGRNLAHQLRDHLH--PSLSSSSSSPSS----NGILLIDQILRSYENALSVLAG 119
           L+ EL  G + A +L+  L   PS SSS SSP++       +L+ QI+ SYE +L +L  
Sbjct: 14  LLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSLLLLNW 73

Query: 120 GSSGGDEEIPPTIAAV---------------------------------KHRD----VSK 179
            SS   + IP  +  V                                  HR      SK
Sbjct: 74  SSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDYIFNSK 133

Query: 180 KRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGCSA 239
           KRK++ KWSE V++     +    DD FSWRKYGQKDILG+KFPR Y+RC+HR  Q C A
Sbjct: 134 KRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHRSTQNCWA 193

Query: 240 TKQVQRLDNDPSMYEITYRGKHTCNKPLHSNTPKQEQTPQLQQHPMLMPPKQEQEEPLQQ 286
           TKQVQR D D +++E+TYRG HTC++ +    P    +P+ +Q   + P   ++ + +  
Sbjct: 194 TKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPL--ASPEKRQDTRVKPAITQKPKDI-L 253

BLAST of CmoCh08G001250.1 vs. TAIR 10
Match: AT2G46400.1 (WRKY DNA-binding protein 46 )

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-28
Identity = 75/169 (44.38%), Postives = 100/169 (59.17%), Query Frame = 0

Query: 54  IMEFVNLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVLAG 113
           +ME   +I EL  G+ LA++L ++L       +SS  SN  L+ D ILR Y+NA+ +L+ 
Sbjct: 2   MMEEKLVINELELGKELANRLMNNL-----KHTSSVDSNKTLISD-ILRIYQNAIFMLSF 61

Query: 114 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKV-PSVSTIGALPDDGFSWRKYGQK 173
                  +    I     ++V KKRKV  K +E VKV  +        DDG  WRKYGQK
Sbjct: 62  NQDKNILKRSLEIDGKDSKNVFKKRKVSEKNTEKVKVFVATEQENGSIDDGHCWRKYGQK 121

Query: 174 DILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCN 222
           +I GSK PR Y+RC+HRF Q C A KQVQ+ D DPS++E+ Y G HTCN
Sbjct: 122 EIHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTCN 164

BLAST of CmoCh08G001250.1 vs. TAIR 10
Match: AT4G11070.1 (WRKY family transcription factor )

HSP 1 Score: 122.9 bits (307), Expect = 7.6e-28
Identity = 102/323 (31.58%), Postives = 155/323 (47.99%), Query Frame = 0

Query: 59  NLIQELNQGRNLAHQLRDHLHPSLSSSSSSPSSN-GILLIDQILRSYENALSVLAGGSSG 118
           +L+ EL  G   A QL+    PSLS+SSS  ++     L+  I+ S++ A+ +L G ++ 
Sbjct: 10  SLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNGSTTQ 69

Query: 119 GDEEI----------------PPTIAA---------VKHRDV---SKKRKVMAKWSELVK 178
            +  I                P +I           V+ ++    SKKRK++ KW+E V+
Sbjct: 70  HNPTIELAPDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTEQVR 129

Query: 179 VPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSM 238
           +     +    DD FSWRKYGQKDILG+KFPR Y+RC+ R  Q C ATKQVQR D DP++
Sbjct: 130 ISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPTI 189

Query: 239 YEITYRGKHTCNKPLHSNTPKQEQTPQLQQHPMLMPPKQEQEEPLQQQDAPLYLTVSSDN 298
           +E+TYRG HTC++ +    P++ +T            K +    +  Q+    LTV +  
Sbjct: 190 FEVTYRGTHTCSQGI--PLPEKRET------------KPKHTVAVNYQNLRASLTVRTGG 249

Query: 299 VDDSLRRPFCPPSPLFRSEVEDDQSPFRGSELEGLEFWCIGDGDLVSGPSSFTNISMGDL 351
           +  S    F   SPL+  E  +    F         +  +G     SGPS FT +   + 
Sbjct: 250 L-GSEAFSFPVTSPLYTYESINGGGTF---------YHHVGS----SGPSDFTGLISTNT 304

BLAST of CmoCh08G001250.1 vs. TAIR 10
Match: AT5G24110.1 (WRKY DNA-binding protein 30 )

HSP 1 Score: 121.7 bits (304), Expect = 1.7e-27
Identity = 73/190 (38.42%), Postives = 106/190 (55.79%), Query Frame = 0

Query: 65  NQGRNLAHQLRDHLHPSLSSSSSSPSSNGILLIDQILRSYENALSVL-----------AG 124
           N+   L  + RD+ H   S+SS     +   L  +IL+SY  +L+++            G
Sbjct: 15  NEINELMIEGRDYAHQFGSASSQETREH---LAKKILQSYHKSLTIMNYSGELDQVSQGG 74

Query: 125 GSSGGDEEIPPTIAAVKHRDVSKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKD 184
           GS   D+     +       +   +K M +WS  V++   + +    DDGFSWRKYGQKD
Sbjct: 75  GSPKSDDSDQEPLV------IKSSKKSMPRWSSKVRIAPGAGVDRTLDDGFSWRKYGQKD 134

Query: 185 ILGSKFPRGYFRCSHRFAQGCSATKQVQRLDNDPSMYEITYRGKHTCNKPLHSNT--PKQ 241
           ILG+KFPRGY+RC++R +QGC ATKQVQR D +  + EI+YRG H+C++  +  T  P Q
Sbjct: 135 ILGAKFPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRGIHSCSQAANVGTTMPIQ 194

BLAST of CmoCh08G001250.1 vs. TAIR 10
Match: AT4G11070.2 (WRKY family transcription factor )

HSP 1 Score: 118.2 bits (295), Expect = 1.9e-26
Identity = 52/88 (59.09%), Postives = 67/88 (76.14%), Query Frame = 0

Query: 135 SKKRKVMAKWSELVKVPSVSTIGALPDDGFSWRKYGQKDILGSKFPRGYFRCSHRFAQGC 194
           SKKRK++ KW+E V++     +    DD FSWRKYGQKDILG+KFPR Y+RC+ R  Q C
Sbjct: 83  SKKRKMLPKWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYC 142

Query: 195 SATKQVQRLDNDPSMYEITYRGKHTCNK 223
            ATKQVQR D DP+++E+TYRG HTC++
Sbjct: 143 WATKQVQRSDGDPTIFEVTYRGTHTCSQ 170

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUP63.5e-3035.69Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 ... [more]
Q9SKD91.6e-2744.38Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 ... [more]
Q8H0Y81.1e-2631.58Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 ... [more]
Q9FL622.4e-2638.42Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 ... [more]
Q0DFT75.8e-2537.50Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 P... [more]
Match NameE-valueIdentityDescription
A0A6J1H6D37.4e-156100.00probable WRKY transcription factor 53 OS=Cucurbita moschata OX=3662 GN=LOC111460... [more]
A0A6J1KTV71.2e-14293.26probable WRKY transcription factor 53 OS=Cucurbita maxima OX=3661 GN=LOC11149816... [more]
A0A5D3C9479.2e-8260.00Putative WRKY transcription factor 53 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CGE21.2e-8160.00probable WRKY transcription factor 53 OS=Cucumis melo OX=3656 GN=LOC103500461 PE... [more]
E7CEY44.7e-7857.97WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY50 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022959976.11.5e-155100.00probable WRKY transcription factor 53 [Cucurbita moschata][more]
KAG7025317.12.1e-14997.47putative WRKY transcription factor 46, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_023513678.11.2e-14796.09probable WRKY transcription factor 53 [Cucurbita pepo subsp. pepo][more]
KAG6592912.17.6e-14795.74putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023005056.12.5e-14293.26probable WRKY transcription factor 53 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G23810.12.5e-3135.69WRKY family transcription factor [more]
AT2G46400.11.2e-2844.38WRKY DNA-binding protein 46 [more]
AT4G11070.17.6e-2831.58WRKY family transcription factor [more]
AT5G24110.11.7e-2738.42WRKY DNA-binding protein 30 [more]
AT4G11070.21.9e-2659.09WRKY family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 160..222
e-value: 1.1E-41
score: 154.4
IPR003657WRKY domainPFAMPF03106WRKYcoord: 161..221
e-value: 1.7E-26
score: 92.2
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 155..223
score: 23.536766
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 148..224
e-value: 1.1E-28
score: 100.9
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 159..223
NoneNo IPR availablePANTHERPTHR32096:SF102WRKY TRANSCRIPTION FACTOR 46-RELATEDcoord: 55..332
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 55..332

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh08G001250CmoCh08G001250gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh08G001250.1:exon:5267CmoCh08G001250.1:exon:5267exon
CmoCh08G001250.1:exon:5266CmoCh08G001250.1:exon:5266exon
CmoCh08G001250.1:exon:5265CmoCh08G001250.1:exon:5265exon
CmoCh08G001250.1:exon:5264CmoCh08G001250.1:exon:5264exon
CmoCh08G001250.1:exon:5263CmoCh08G001250.1:exon:5263exon
CmoCh08G001250.1:exon:5262CmoCh08G001250.1:exon:5262exon
CmoCh08G001250.1:exon:5261CmoCh08G001250.1:exon:5261exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_7CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_6CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_5CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_4CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_3CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cds_2CDS
CmoCh08G001250.1:cdsCmoCh08G001250.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh08G001250.1:five_prime_utrCmoCh08G001250.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh08G001250.1CmoCh08G001250.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0048583 regulation of response to stimulus
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0000976 transcription cis-regulatory region binding