Cmc04g0098971.1 (mRNA) Melon (Charmono) v1.1

Overview


Name	Cmc04g0098971.1
Type	mRNA
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Ribonuclease H
Location	CMiso1.1chr04: 14723658 .. 14724416 (-)
Sequence length	759
RNA-Seq Expression	Cmc04g0098971.1
Synteny	Cmc04g0098971.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

mRNA sequence

ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

Coding sequence (CDS)

ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

Protein sequence

MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVVKKVF

Homology

[Close]

BLAST of Cmc04g0098971.1 vs. NCBI nr
Match: KAA0038143.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 498.4 bits (1282), Expect = 3.5e-137
Identity = 244/252 (96.83%), Postives = 244/252 (96.83%), Query Frame = 0


Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI
Sbjct: 1   MDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMN AIEAANKNIKRIIEKMTITYKDWH
Sbjct: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWH 120

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLL FQKDHR KWTP
Sbjct: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLPFQKDHREKWTP 240

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 241 NYEGPFVVKKAF 252

[Close]

BLAST of Cmc04g0098971.1 vs. NCBI nr
Match: KAA0037621.1 (uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.1e-133
Identity = 236/252 (93.65%), Postives = 241/252 (95.63%), Query Frame = 0


Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            MDVIGPI PK SN HRF LV+IDYFTKWIE AS+CNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1658 MDVIGPIDPKASNGHRFTLVSIDYFTKWIEVASFCNVTRGVVLKFIKKELICRYGLPEGI 1717

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNGA+EAANKNIKRIIEKMTITYKDWH
Sbjct: 1718 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTITYKDWH 1777

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 1778 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 1837

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNFVEEK+LAALNH QLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 1838 EQLNFVEEKRLAALNHVQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTP 1897

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 1898 NYEGPFVVKKAF 1909

[Close]

BLAST of Cmc04g0098971.1 vs. NCBI nr
Match: XP_031738551.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0


Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 2355 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 2414

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 2415 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2474

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2475 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2534

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2535 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2594

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2595 NYEGPFVVKKAF 2606

[Close]

BLAST of Cmc04g0098971.1 vs. NCBI nr
Match: XP_031738857.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116402780 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0


Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 2023 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 2082

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 2083 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2142

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2143 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2202

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2203 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2262

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2263 NYEGPFVVKKAF 2274

[Close]

BLAST of Cmc04g0098971.1 vs. NCBI nr
Match: XP_031742240.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0


Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1931 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 1990

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 1991 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2050

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2051 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2110

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2111 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2170

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2171 NYEGPFVVKKAF 2182

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot
Match: P03360 (Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=pol PE=1 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-15
Identity = 45/137 (32.85%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV+K +  ++I R+GLP  I +DN      K+  
Sbjct: 885  YKYLLVLVDTFSGWVEAYPAKRETSQVVIKHLIHDIIPRFGLPVQIGSDNGPAFVAKVTQ 944

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTI-TYKDWHEMLPFALHGYRTS 134
            +LCE   ++ +    Y P+ +G +E  N+ +K  I K+ I T  DW  +LP AL   R +
Sbjct: 945  QLCEALNVSWKLHCAYRPQSSGQVERMNRTLKETIAKLRIETGGDWVSLLPQALLRARCT 1004

Query: 135  VRTSTGATPFSLVYGME 151
                 G +PF ++YG++
Sbjct: 1005 PGRE-GLSPFEILYGLK 1020

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot
Match: P26809 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=pol PE=3 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-15
Identity = 63/236 (26.69%), Postives = 112/236 (47.46%), Query Frame = 0


Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV ID F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1466 YKYLLVFIDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1525

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1526 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1585

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1586 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1645

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1646 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1683

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot
Match: P26810 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.6e-15
Identity = 62/236 (26.27%), Postives = 112/236 (47.46%), Query Frame = 0


Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1467 YKYLLVFVDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1526

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1527 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1586

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1587 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1646

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1647 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1684

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot
Match: P26808 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate PVC-211) OX=11798 GN=pol PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.6e-15
Identity = 62/236 (26.27%), Postives = 112/236 (47.46%), Query Frame = 0


Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1466 YKYLLVFVDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1525

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1526 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1585

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1586 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1645

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1646 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1683

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot
Match: Q2F7J3 (Gag-Pol polyprotein OS=Xenotropic MuLV-related virus (isolate VP35) OX=356663 GN=gag-pol PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.1e-15
Identity = 61/239 (25.52%), Postives = 114/239 (47.70%), Query Frame = 0


Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +++  R+G+P+ + +DN     +++  
Sbjct: 1464 YKYLLVFVDTFSGWVEAFPTKRETAKVVTKKLLEDIFPRFGMPQVLGSDNGPAFASQVSQ 1523

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   I+ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1524 SVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLASGTRDWVLLLPLALYRARN 1583

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEE---K 194
            +     G TP+ ++YG    L +    P +     +KL  +  ++   + L  V++   K
Sbjct: 1584 TPGPH-GLTPYEILYGAPPPL-VNFHDPEM-----SKLTNSPSLQAHLQALQAVQQEVWK 1643

Query: 195  QLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             LAA    QL         ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1644 PLAAAYQDQL---------DQPVIPHPFRVGDAVWVR-----RHQTKNLEPRWKGPYTV 1681

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL
Match: A0A5A7T570 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003710 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-137
Identity = 244/252 (96.83%), Postives = 244/252 (96.83%), Query Frame = 0


Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI
Sbjct: 1   MDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMN AIEAANKNIKRIIEKMTITYKDWH
Sbjct: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWH 120

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLL FQKDHR KWTP
Sbjct: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLPFQKDHREKWTP 240

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 241 NYEGPFVVKKAF 252

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL
Match: A0A5A7T7R4 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002820 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.2e-134
Identity = 236/252 (93.65%), Postives = 241/252 (95.63%), Query Frame = 0


Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            MDVIGPI PK SN HRF LV+IDYFTKWIE AS+CNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1658 MDVIGPIDPKASNGHRFTLVSIDYFTKWIEVASFCNVTRGVVLKFIKKELICRYGLPEGI 1717

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNGA+EAANKNIKRIIEKMTITYKDWH
Sbjct: 1718 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTITYKDWH 1777

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 1778 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 1837

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNFVEEK+LAALNH QLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 1838 EQLNFVEEKRLAALNHVQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTP 1897

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 1898 NYEGPFVVKKAF 1909

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL
Match: A0A5A7T1D0 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002530 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 6.1e-127
Identity = 228/252 (90.48%), Postives = 231/252 (91.67%), Query Frame = 0


Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PKTSN HRFILV IDYFTKWIEAASY NVTR VVLKFIKKE ICRYGL E I
Sbjct: 89  MDVIGPIDPKTSNGHRFILVVIDYFTKWIEAASYYNVTRGVVLKFIKKEFICRYGLLECI 148

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNG +E ANKNIKRIIEKMTITYKDWH
Sbjct: 149 ITDNAKNLNNKMMDELCEQFKINHRNSTPYLPKMNGTVEEANKNIKRIIEKMTITYKDWH 208

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGA PF LVYGME VLPLEVE PSLRVL+EAKLDEAEWIRGRY
Sbjct: 209 EMLPFALHGYRTSVRTSTGAKPFFLVYGMEVVLPLEVETPSLRVLIEAKLDEAEWIRGRY 268

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQL ALNH QLYQRRLMRAYNKKVH RSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 269 EQLNFVEEKQLTALNHRQLYQRRLMRAYNKKVHTRSFREGDLVLKRILPFQKDHRGKWTP 328

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 329 NYEGPFVVKKAF 340

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL
Match: A0A5D3E1A3 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00260 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.2e-126
Identity = 226/252 (89.68%), Postives = 229/252 (90.87%), Query Frame = 0


Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PKTSN H FILV IDYFTKWIEAASY NV R VVLKFIKKE ICRYGL E I
Sbjct: 89  MDVIGPIDPKTSNGHHFILVVIDYFTKWIEAASYYNVIRGVVLKFIKKEFICRYGLLECI 148

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNG +E ANKNIKRIIEKMTITYKDWH
Sbjct: 149 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGTVEEANKNIKRIIEKMTITYKDWH 208

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGA PF LVYGME VLPLEVE PSLRVL+EAKLDEAEWIRGRY
Sbjct: 209 EMLPFALHGYRTSVRTSTGAKPFFLVYGMEVVLPLEVETPSLRVLIEAKLDEAEWIRGRY 268

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQL ALNH QLYQRRLMRAYNKKVH RSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 269 EQLNFVEEKQLTALNHRQLYQRRLMRAYNKKVHTRSFREGDLVLKRILPFQKDHRGKWTP 328

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 329 NYEGPFVVKKAF 340

[Close]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL
Match: A0A5A7VCB5 (Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G00520 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.2e-122
Identity = 229/297 (77.10%), Postives = 236/297 (79.46%), Query Frame = 0


Query: 1   MDVIGPIVPKTSNSHRFILVA--------------------------------------- 60
           M+VIGPI PK SN HRFILV                                        
Sbjct: 178 MNVIGPIDPKASNGHRFILVVMVSQRIEAASYCNVTRGVVLKFIKKELICRYGLPESIIT 237

Query: 61  ------IDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMDE 120
                 IDYFTKWIE ASYCNVTR VVLKFIKKEL CRYGLPEGIITDN KNLNNKMMDE
Sbjct: 238 DNAKNLIDYFTKWIEVASYCNVTRGVVLKFIKKELKCRYGLPEGIITDNVKNLNNKMMDE 297

Query: 121 LCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVR 180
           LCEQFKINH+NST Y PKMNGA+EAANKNIKRIIEKMTIT+KDWHEMLPFALHGY  SVR
Sbjct: 298 LCEQFKINHQNSTLYRPKMNGAVEAANKNIKRIIEKMTITHKDWHEMLPFALHGYCMSVR 357

Query: 181 TSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLAALN 240
           TSTGATPFSL YGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEK+LAALN
Sbjct: 358 TSTGATPFSLFYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKRLAALN 417

Query: 241 HGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVVKKVF 253
           HGQLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTPNYEGPFVVK +F
Sbjct: 418 HGQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKTLF 474

The following BLAST results are available for this feature:

BLAST of Cmc04g0098971.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038143.1	3.5e-137	96.83	Pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0037621.1	1.1e-133	93.65	uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa]	[more]
XP_031738551.1	2.6e-132	92.06	LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus]	[more]
XP_031738857.1	2.6e-132	92.06	LOW QUALITY PROTEIN: uncharacterized protein LOC116402780 [Cucumis sativus]	[more]
XP_031742240.1	2.6e-132	92.06	LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus]	[more]

BLAST of Cmc04g0098971.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P03360	2.7e-15	32.85	Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=...	[more]
P26809	2.7e-15	26.69	Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=p...	[more]
P26810	3.6e-15	26.27	Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol...	[more]
P26808	3.6e-15	26.27	Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate PVC-211) OX=11798 G...	[more]
Q2F7J3	6.1e-15	25.52	Gag-Pol polyprotein OS=Xenotropic MuLV-related virus (isolate VP35) OX=356663 GN...	[more]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T570	1.7e-137	96.83	Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G00371...	[more]
A0A5A7T7R4	5.2e-134	93.65	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G00282...	[more]
A0A5A7T1D0	6.1e-127	90.48	RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A5D3E1A3	5.2e-126	89.68	RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A5A7VCB5	1.2e-122	77.10	Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=...	[more]

BLAST of Cmc04g0098971.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..187 e-value: 7.8E-49 score: 167.7
IPR001584	Integrase, catalytic core	PFAM	PF00665	rve	coord: 10..92 e-value: 9.8E-10 score: 38.7
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..150 score: 20.122492
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 31..234
None	No IPR available	PANTHER	PTHR24559:SF342	SUBFAMILY NOT NAMED	coord: 31..234
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 1..156

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cmc04g0098971	Cmc04g0098971	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0098971.1-exon	Cmc04g0098971.1-exon-CMiso1.1chr04:14723658..14724416	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc04g0098971.1-cds	Cmc04g0098971.1-cds-CMiso1.1chr04:14723658..14724416	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cmc04g0098971.1	Cmc04g0098971.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity