Cmc04g0098971 (gene) Melon (Charmono) v1.1

Overview
NameCmc04g0098971
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionRibonuclease H
LocationCMiso1.1chr04: 14723658 .. 14724416 (-)
RNA-Seq ExpressionCmc04g0098971
SyntenyCmc04g0098971
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

mRNA sequence

ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

Coding sequence (CDS)

ATGGATGTTATTGGACCCATTGTTCCTAAAACCTCAAATAGCCATCGTTTTATTCTTGTGGCCATTGATTACTTCACTAAATGGATAGAGGCTGCATCTTATTGCAATGTTACAAGGAGAGTAGTGCTCAAGTTTATCAAGAAAGAGCTTATATGTCGTTATGGTCTCCCAGAAGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGCAGTTCAAGATCAATCACCGAAATTCGACTCCATATCACCCAAAGATGAATGGGGCAATTGAGGCAGCTAACAAAAATATTAAAAGAATCATTGAGAAAATGACAATAACATACAAAGATTGGCATGAAATGCTACCGTTTGCGTTGCATGGATATCGCACATCAGTTCGTACTTCAACAGGAGCAACACCATTTTCTTTAGTTTATGGTATGGAAGCTGTTTTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAAGCTGAATGGATACGAGGTCGTTATGAGCAGTTGAATTTCGTTGAAGAAAAACAGTTGGCAGCATTAAATCATGGGCAACTTTACCAAAGAAGACTAATGCGAGCATACAATAAGAAAGTGCATCCTCGAAGCTTTCGAGAGGAAGATTTGGTATTAAAAAGATTACTTTCGTTTCAAAAGGATCATCGAGGAAAATGGACTCCTAATTATGAAGGACCATTTGTAGTGAAAAAAGTTTTTTAG

Protein sequence

MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVVKKVF
Homology
BLAST of Cmc04g0098971 vs. NCBI nr
Match: KAA0038143.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 498.4 bits (1282), Expect = 3.5e-137
Identity = 244/252 (96.83%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI
Sbjct: 1   MDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMN AIEAANKNIKRIIEKMTITYKDWH
Sbjct: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWH 120

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLL FQKDHR KWTP
Sbjct: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLPFQKDHREKWTP 240

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 241 NYEGPFVVKKAF 252

BLAST of Cmc04g0098971 vs. NCBI nr
Match: KAA0037621.1 (uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.1e-133
Identity = 236/252 (93.65%), Postives = 241/252 (95.63%), Query Frame = 0

Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            MDVIGPI PK SN HRF LV+IDYFTKWIE AS+CNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1658 MDVIGPIDPKASNGHRFTLVSIDYFTKWIEVASFCNVTRGVVLKFIKKELICRYGLPEGI 1717

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNGA+EAANKNIKRIIEKMTITYKDWH
Sbjct: 1718 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTITYKDWH 1777

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 1778 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 1837

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNFVEEK+LAALNH QLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 1838 EQLNFVEEKRLAALNHVQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTP 1897

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 1898 NYEGPFVVKKAF 1909

BLAST of Cmc04g0098971 vs. NCBI nr
Match: XP_031738551.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0

Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 2355 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 2414

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 2415 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2474

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2475 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2534

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2535 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2594

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2595 NYEGPFVVKKAF 2606

BLAST of Cmc04g0098971 vs. NCBI nr
Match: XP_031738857.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116402780 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0

Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 2023 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 2082

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 2083 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2142

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2143 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2202

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2203 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2262

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2263 NYEGPFVVKKAF 2274

BLAST of Cmc04g0098971 vs. NCBI nr
Match: XP_031742240.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 232/252 (92.06%), Postives = 241/252 (95.63%), Query Frame = 0

Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            +DVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1931 IDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVVLKFIKKELICRYGLPEGI 1990

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCE+FKINHRNSTPY PKMNGA+EAANKNIKRIIEKMT TYKDWH
Sbjct: 1991 ITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKDWH 2050

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            E+LPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 2051 EILPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 2110

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNF+EEK+L AL+HGQLYQ+RLMRAYNKKVHPRSFRE DLVLKR+L F KDHRGKWTP
Sbjct: 2111 EQLNFIEEKRLTALSHGQLYQKRLMRAYNKKVHPRSFREGDLVLKRILPFLKDHRGKWTP 2170

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 2171 NYEGPFVVKKAF 2182

BLAST of Cmc04g0098971 vs. ExPASy Swiss-Prot
Match: P03360 (Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=pol PE=1 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-15
Identity = 45/137 (32.85%), Postives = 77/137 (56.20%), Query Frame = 0

Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV+K +  ++I R+GLP  I +DN      K+  
Sbjct: 885  YKYLLVLVDTFSGWVEAYPAKRETSQVVIKHLIHDIIPRFGLPVQIGSDNGPAFVAKVTQ 944

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTI-TYKDWHEMLPFALHGYRTS 134
            +LCE   ++ +    Y P+ +G +E  N+ +K  I K+ I T  DW  +LP AL   R +
Sbjct: 945  QLCEALNVSWKLHCAYRPQSSGQVERMNRTLKETIAKLRIETGGDWVSLLPQALLRARCT 1004

Query: 135  VRTSTGATPFSLVYGME 151
                 G +PF ++YG++
Sbjct: 1005 PGRE-GLSPFEILYGLK 1020

BLAST of Cmc04g0098971 vs. ExPASy Swiss-Prot
Match: P26809 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=pol PE=3 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-15
Identity = 63/236 (26.69%), Postives = 112/236 (47.46%), Query Frame = 0

Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV ID F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1466 YKYLLVFIDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1525

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1526 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1585

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1586 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1645

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1646 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1683

BLAST of Cmc04g0098971 vs. ExPASy Swiss-Prot
Match: P26810 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.6e-15
Identity = 62/236 (26.27%), Postives = 112/236 (47.46%), Query Frame = 0

Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1467 YKYLLVFVDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1526

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1527 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1586

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1587 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1646

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1647 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1684

BLAST of Cmc04g0098971 vs. ExPASy Swiss-Prot
Match: P26808 (Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate PVC-211) OX=11798 GN=pol PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 3.6e-15
Identity = 62/236 (26.27%), Postives = 112/236 (47.46%), Query Frame = 0

Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +E+  R+G+P+ + TDN     +K+  
Sbjct: 1466 YKYLLVFVDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVSQ 1525

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   ++ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1526 TVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRARN 1585

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLA 194
            +     G TP+ ++YG     P  V  P   +   AK+     ++   + L  V+ +   
Sbjct: 1586 TPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVWR 1645

Query: 195  ALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             L     YQ +L    ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1646 PL--AAAYQEQL----DRPVVPHPFRVGDTVWVR-----RHQTKNLEPRWKGPYTV 1683

BLAST of Cmc04g0098971 vs. ExPASy Swiss-Prot
Match: Q2F7J3 (Gag-Pol polyprotein OS=Xenotropic MuLV-related virus (isolate VP35) OX=356663 GN=gag-pol PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.1e-15
Identity = 61/239 (25.52%), Postives = 114/239 (47.70%), Query Frame = 0

Query: 15   HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMD 74
            ++++LV +D F+ W+EA      T +VV K + +++  R+G+P+ + +DN     +++  
Sbjct: 1464 YKYLLVFVDTFSGWVEAFPTKRETAKVVTKKLLEDIFPRFGMPQVLGSDNGPAFASQVSQ 1523

Query: 75   ELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTIT--YKDWHEMLPFALHGYRT 134
             + +   I+ +    Y P+ +G +E  N+ IK  + K+T+    +DW  +LP AL+  R 
Sbjct: 1524 SVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLASGTRDWVLLLPLALYRARN 1583

Query: 135  SVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEE---K 194
            +     G TP+ ++YG    L +    P +     +KL  +  ++   + L  V++   K
Sbjct: 1584 TPGPH-GLTPYEILYGAPPPL-VNFHDPEM-----SKLTNSPSLQAHLQALQAVQQEVWK 1643

Query: 195  QLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVV 249
             LAA    QL         ++ V P  FR  D V  R     +       P ++GP+ V
Sbjct: 1644 PLAAAYQDQL---------DQPVIPHPFRVGDAVWVR-----RHQTKNLEPRWKGPYTV 1681

BLAST of Cmc04g0098971 vs. ExPASy TrEMBL
Match: A0A5A7T570 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003710 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.7e-137
Identity = 244/252 (96.83%), Postives = 244/252 (96.83%), Query Frame = 0

Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PK SN HRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI
Sbjct: 1   MDVIGPIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMN AIEAANKNIKRIIEKMTITYKDWH
Sbjct: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAANKNIKRIIEKMTITYKDWH 120

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLL FQKDHR KWTP
Sbjct: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLPFQKDHREKWTP 240

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 241 NYEGPFVVKKAF 252

BLAST of Cmc04g0098971 vs. ExPASy TrEMBL
Match: A0A5A7T7R4 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002820 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.2e-134
Identity = 236/252 (93.65%), Postives = 241/252 (95.63%), Query Frame = 0

Query: 1    MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
            MDVIGPI PK SN HRF LV+IDYFTKWIE AS+CNVTR VVLKFIKKELICRYGLPEGI
Sbjct: 1658 MDVIGPIDPKASNGHRFTLVSIDYFTKWIEVASFCNVTRGVVLKFIKKELICRYGLPEGI 1717

Query: 61   ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
            ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNGA+EAANKNIKRIIEKMTITYKDWH
Sbjct: 1718 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTITYKDWH 1777

Query: 121  EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
            EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY
Sbjct: 1778 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 1837

Query: 181  EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
            EQLNFVEEK+LAALNH QLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 1838 EQLNFVEEKRLAALNHVQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTP 1897

Query: 241  NYEGPFVVKKVF 253
            NYEGPFVVKK F
Sbjct: 1898 NYEGPFVVKKAF 1909

BLAST of Cmc04g0098971 vs. ExPASy TrEMBL
Match: A0A5A7T1D0 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002530 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 6.1e-127
Identity = 228/252 (90.48%), Postives = 231/252 (91.67%), Query Frame = 0

Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PKTSN HRFILV IDYFTKWIEAASY NVTR VVLKFIKKE ICRYGL E I
Sbjct: 89  MDVIGPIDPKTSNGHRFILVVIDYFTKWIEAASYYNVTRGVVLKFIKKEFICRYGLLECI 148

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNG +E ANKNIKRIIEKMTITYKDWH
Sbjct: 149 ITDNAKNLNNKMMDELCEQFKINHRNSTPYLPKMNGTVEEANKNIKRIIEKMTITYKDWH 208

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGA PF LVYGME VLPLEVE PSLRVL+EAKLDEAEWIRGRY
Sbjct: 209 EMLPFALHGYRTSVRTSTGAKPFFLVYGMEVVLPLEVETPSLRVLIEAKLDEAEWIRGRY 268

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQL ALNH QLYQRRLMRAYNKKVH RSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 269 EQLNFVEEKQLTALNHRQLYQRRLMRAYNKKVHTRSFREGDLVLKRILPFQKDHRGKWTP 328

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 329 NYEGPFVVKKAF 340

BLAST of Cmc04g0098971 vs. ExPASy TrEMBL
Match: A0A5D3E1A3 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00260 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.2e-126
Identity = 226/252 (89.68%), Postives = 229/252 (90.87%), Query Frame = 0

Query: 1   MDVIGPIVPKTSNSHRFILVAIDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGI 60
           MDVIGPI PKTSN H FILV IDYFTKWIEAASY NV R VVLKFIKKE ICRYGL E I
Sbjct: 89  MDVIGPIDPKTSNGHHFILVVIDYFTKWIEAASYYNVIRGVVLKFIKKEFICRYGLLECI 148

Query: 61  ITDNAKNLNNKMMDELCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWH 120
           ITDNAKNLNNKMMDELCEQFKINHRNSTPY PKMNG +E ANKNIKRIIEKMTITYKDWH
Sbjct: 149 ITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGTVEEANKNIKRIIEKMTITYKDWH 208

Query: 121 EMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRY 180
           EMLPFALHGYRTSVRTSTGA PF LVYGME VLPLEVE PSLRVL+EAKLDEAEWIRGRY
Sbjct: 209 EMLPFALHGYRTSVRTSTGAKPFFLVYGMEVVLPLEVETPSLRVLIEAKLDEAEWIRGRY 268

Query: 181 EQLNFVEEKQLAALNHGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTP 240
           EQLNFVEEKQL ALNH QLYQRRLMRAYNKKVH RSFRE DLVLKR+L FQKDHRGKWTP
Sbjct: 269 EQLNFVEEKQLTALNHRQLYQRRLMRAYNKKVHTRSFREGDLVLKRILPFQKDHRGKWTP 328

Query: 241 NYEGPFVVKKVF 253
           NYEGPFVVKK F
Sbjct: 329 NYEGPFVVKKAF 340

BLAST of Cmc04g0098971 vs. ExPASy TrEMBL
Match: A0A5A7VCB5 (Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G00520 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.2e-122
Identity = 229/297 (77.10%), Postives = 236/297 (79.46%), Query Frame = 0

Query: 1   MDVIGPIVPKTSNSHRFILVA--------------------------------------- 60
           M+VIGPI PK SN HRFILV                                        
Sbjct: 178 MNVIGPIDPKASNGHRFILVVMVSQRIEAASYCNVTRGVVLKFIKKELICRYGLPESIIT 237

Query: 61  ------IDYFTKWIEAASYCNVTRRVVLKFIKKELICRYGLPEGIITDNAKNLNNKMMDE 120
                 IDYFTKWIE ASYCNVTR VVLKFIKKEL CRYGLPEGIITDN KNLNNKMMDE
Sbjct: 238 DNAKNLIDYFTKWIEVASYCNVTRGVVLKFIKKELKCRYGLPEGIITDNVKNLNNKMMDE 297

Query: 121 LCEQFKINHRNSTPYHPKMNGAIEAANKNIKRIIEKMTITYKDWHEMLPFALHGYRTSVR 180
           LCEQFKINH+NST Y PKMNGA+EAANKNIKRIIEKMTIT+KDWHEMLPFALHGY  SVR
Sbjct: 298 LCEQFKINHQNSTLYRPKMNGAVEAANKNIKRIIEKMTITHKDWHEMLPFALHGYCMSVR 357

Query: 181 TSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKQLAALN 240
           TSTGATPFSL YGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEK+LAALN
Sbjct: 358 TSTGATPFSLFYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGRYEQLNFVEEKRLAALN 417

Query: 241 HGQLYQRRLMRAYNKKVHPRSFREEDLVLKRLLSFQKDHRGKWTPNYEGPFVVKKVF 253
           HGQLYQRRLMRAYNKKVHPRSFRE DLVLKR+L FQKDHRGKWTPNYEGPFVVK +F
Sbjct: 418 HGQLYQRRLMRAYNKKVHPRSFREGDLVLKRILPFQKDHRGKWTPNYEGPFVVKTLF 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0038143.13.5e-13796.83Pol polyprotein [Cucumis melo var. makuwa][more]
KAA0037621.11.1e-13393.65uncharacterized protein E6C27_scaffold277G002820 [Cucumis melo var. makuwa][more]
XP_031738551.12.6e-13292.06LOW QUALITY PROTEIN: uncharacterized protein LOC101203611 [Cucumis sativus][more]
XP_031738857.12.6e-13292.06LOW QUALITY PROTEIN: uncharacterized protein LOC116402780 [Cucumis sativus][more]
XP_031742240.12.6e-13292.06LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P033602.7e-1532.85Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=... [more]
P268092.7e-1526.69Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) OX=11797 GN=p... [more]
P268103.6e-1526.27Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate 57) OX=11796 GN=pol... [more]
P268083.6e-1526.27Gag-Pol polyprotein OS=Friend murine leukemia virus (isolate PVC-211) OX=11798 G... [more]
Q2F7J36.1e-1525.52Gag-Pol polyprotein OS=Xenotropic MuLV-related virus (isolate VP35) OX=356663 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7T5701.7e-13796.83Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G00371... [more]
A0A5A7T7R45.2e-13493.65Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G00282... [more]
A0A5A7T1D06.1e-12790.48RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A5D3E1A35.2e-12689.68RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7VCB51.2e-12277.10Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..187
e-value: 7.8E-49
score: 167.7
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 10..92
e-value: 9.8E-10
score: 38.7
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 1..150
score: 20.122492
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 31..234
NoneNo IPR availablePANTHERPTHR24559:SF342SUBFAMILY NOT NAMEDcoord: 31..234
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 1..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc04g0098971.1Cmc04g0098971.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006310 DNA recombination
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0003676 nucleic acid binding