CmaCh20G006570.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G006570.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like
Location	Cma_Chr20: 3029826 .. 3032131 (+)
Sequence length	606
RNA-Seq Expression	CmaCh20G006570.1
Synteny	CmaCh20G006570.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGCGTGAGTTAATCAGTGGCGGCTGTGGTGCAGAGGATGAAGCGTGCTGCTTCGCGTTCACAGCCAATTGCCGTAACGAACGACTTGGGTATCGAATCCCAGCGGAGTATTTTGGGAATTGCTTTGCGTTTTGCCTTACGACAGTAGAGAAGAGTGAGCTTTTGGGGGAAAATGGGTTAGTTGTGGCAATGAAAGCGATCGAGAAAAGAGTGGGAGAAGTGAAGAAAGAGACTTTGAAAGACGCAATGGCGGAGACGACCGTGTGGAAGGAATTTGTTGAACGTGGAACTATGCTTTGCGCCGTTGGGTCGCCGAGGTTGGGCGCGTATGACACCAGTTTTGGGTGGGGGAGGCCCATAAAGACCGAGGTGGTTCAAGTTGATCGTGGGAGGTTGTTTTCTATAGGAGAATCCAGGAATGAAAATGGTGGCATTGAAGTTGGGTTGGGTCTGGGTTCGACTCATATGGCTAGCTTTGTTGCCGTGTGGGAACAAAATCTAAAGCTTCTCGGTTGCTCATGACATAATAATAGTATGATATTGTCCACTTTGAATATGAGGCCTCTTAACTTTGCTTTGAGCTTATCATAGGTCTCACAACAGTGAAGAGAGTATTATTTGATTATAAACAGATGATCATTCTCTAAATTAGTTGATGCGGGACTTTAATCATCTAAGTACGCTTCCCCTTAATCAAACCTCGACTCTCTTTTTTTTTTTCCACTCTCATAATTTTATTCAAAACAATATCATACTATATTGGAGAGTCCGGTTCATCTAACAACTTGCATGAAAGTAAACAAAATAACATTTACCTAAGTGGAACTAAAAAAAAAAGACTTTTGGAATAAAAAATAAAATAAATAGTTGATATGACGGCAATGGAATTTAAGGTGAAAAGTAATTTATGTTGAATAAAAATAATAAATGAAGTAATTCGGGGAAGATTTATTGTTGTTAATTACACAAACTCCTTTCTAAATTACTACAATAAAAAAAAATTAATGTAAAAAACAAACCAATTTCTGTTCTTAATTTAACAACTTATTTAATAATACTTTTTTTAACACGTTATTTGGTATTAGTTTTTTTTGTTAGATAATTATTTAATTGTTTTGCTTGGCGAGTGAAGTCCACGCGGCGTAAATGTTAACTGGAATATGAATATTTTCTTTTTTTTTTTTAAGTTAACTGAAGAAATTATGAGCGCGCTGGAGATGGATGTGTAACTATCCGAAACAGGTAATGGCGACGCGCCTTTCTGAATCTTCCCTTCCCTCCACTTTCTCCGTTTATATATATATATAGTATGTTTTTCAGCCCACTGGGGAGAGAGGTGTTTAAGATTTCTTTAATTCTGTTCCGTCAACGCTTTGGTTTTCCGGTGGCTTTTCCGGGTTAGTTCGAACTTTTTTATTTGCTCCGCAGCCTTTTCTCTGATTCTCTTTTCGGAGATTTCTATATTGTTTGTTTTTTTTTGCTTCTTCTGGTATTTTACGGGCATTTCTAGGCGCCGACGGGGAAGACAGAGAATCAGAATAGTAGAATTTTTCTGTTTCGTTGATCTGTTGCTTCTCTGCAATTGTTTAACTCTGGAATTTGAATTTCCACCTGATTTTTTTTTCCTTGGGGGGAGGGGAGAGGGGGGGCATAATGATCTTTACGGATCAGAGATATTCCTTGCGCTTTTTGATGCTTTAACGTCTTGAATATTCTGAATTTTGTCTATTTCGCTTTTCGAATGGGCCATTTTCCCACTTTGAAAGATTCATTGCTGCCGAGCTGAGATTTTTCGTGTAATTTGTATGTAATGTTAATATCTTGTACAATTATGGACATGTAGCATTCGTAATCTATAATGTCGTTAACGTATATGTTGACTTGCAATTTTTGTGCTTAATTGGCAATTCATCACGGGGATTTCTCTGAAAGCGTTACCCTTGTGAATTTTTATTTTATTTTTAGAGTTCTGAAATCGGTTAAGAATCTATACCTAACCTGCTACTCATGATTCATTGCATTTGATATTGAAGATTTGTGGAGTACTGATCAACTGATCACAATGATTGGCATCTATAATCTTTATTTGGGGACTGAATAAGTGATGGGATGAAGTTTCATTTATTTTATTTTCTTATGTTTTTGTTCCCAAATGATTCATGCTACTAGTGTAGAATGGTATTATCTTGAGAAACCTTTGGTATTCACATAACCTACTAATTCCAAAAATTTTGCAATTCATTGATTTATGTGATTTAAGGATATCTATTTTGCTTTGTTGTCTGCATAAATCAGACTCATGA

mRNA sequence

ATGTCGCGTGAGTTAATCAGTGGCGGCTGTGGTGCAGAGGATGAAGCGTGCTGCTTCGCGTTCACAGCCAATTGCCGTAACGAACGACTTGGGTATCGAATCCCAGCGGAGTATTTTGGGAATTGCTTTGCGTTTTGCCTTACGACAGTAGAGAAGAGTGAGCTTTTGGGGGAAAATGGGTTAGTTGTGGCAATGAAAGCGATCGAGAAAAGAGTGGGAGAAGTGAAGAAAGAGACTTTGAAAGACGCAATGGCGGAGACGACCGTGTGGAAGGAATTTGTTGAACGTGGAACTATGCTTTGCGCCGTTGGGTCGCCGAGGTTGGGCGCGTATGACACCAGTTTTGGGTGGGGGAGGCCCATAAAGACCGAGGTGGTTCAAGTTGATCGTGGGAGGTTGTTTTCTATAGGAGAATCCAGGAATGAAAATGGTGGCATTGAAGTTGGGTTGGGTCTGGTTGATATGACGGCAATGGAATTTAAGTTAACTGAAGAAATTATGAGCGCGCTGGAGATGGATGTCCCACTGGGGAGAGAGGTGTTTAAGATTTCTTTAATTCTGTTCCGTCAACGCTTTGGTTTTCCGGTGGCTTTTCCGGACTCATGA

Coding sequence (CDS)

ATGTCGCGTGAGTTAATCAGTGGCGGCTGTGGTGCAGAGGATGAAGCGTGCTGCTTCGCGTTCACAGCCAATTGCCGTAACGAACGACTTGGGTATCGAATCCCAGCGGAGTATTTTGGGAATTGCTTTGCGTTTTGCCTTACGACAGTAGAGAAGAGTGAGCTTTTGGGGGAAAATGGGTTAGTTGTGGCAATGAAAGCGATCGAGAAAAGAGTGGGAGAAGTGAAGAAAGAGACTTTGAAAGACGCAATGGCGGAGACGACCGTGTGGAAGGAATTTGTTGAACGTGGAACTATGCTTTGCGCCGTTGGGTCGCCGAGGTTGGGCGCGTATGACACCAGTTTTGGGTGGGGGAGGCCCATAAAGACCGAGGTGGTTCAAGTTGATCGTGGGAGGTTGTTTTCTATAGGAGAATCCAGGAATGAAAATGGTGGCATTGAAGTTGGGTTGGGTCTGGTTGATATGACGGCAATGGAATTTAAGTTAACTGAAGAAATTATGAGCGCGCTGGAGATGGATGTCCCACTGGGGAGAGAGGTGTTTAAGATTTCTTTAATTCTGTTCCGTCAACGCTTTGGTTTTCCGGTGGCTTTTCCGGACTCATGA

Protein sequence

MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQVDRGRLFSIGESRNENGGIEVGLGLVDMTAMEFKLTEEIMSALEMDVPLGREVFKISLILFRQRFGFPVAFPDS

Homology

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BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot
Match: Q9ZWR8 (Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-17
Identity = 50/143 (34.97%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 13  EDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEKRV 72
           E+E   F+FTA+CR   L    P  YFGNC A C+      EL+G+ GL+VA+ AI + +
Sbjct: 309 ENELEYFSFTADCRG-LLTPPCPPNYFGNCLASCVAKATHKELVGDKGLLVAVAAIGEAI 368

Query: 73  GEVKKETLKDAMAETTVW---KEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQVD 132
            E +    K  +A+   W      +     L   GSP+  +Y   FGWG+P K ++  VD
Sbjct: 369 -EKRLHNEKGVLADAKTWLSESNGIPSKRFLGITGSPKFDSYGVDFGWGKPAKFDITSVD 428

Query: 133 RGRLFSIGESRNENGGIEVGLGL 153
              L  + +SR+   G+E+G+ L
Sbjct: 429 YAELIYVIQSRDFEKGVEIGVSL 449

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BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.0e-17
Identity = 53/147 (36.05%), Postives = 77/147 (52.38%), Query Frame = 0


Query: 12  AEDEACCFAFTANCRNERLGYR--IPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIE 71
           A+DE        +CRN RL Y   IP  YFGNC A  + +V+K +LLGE  ++ A  AI 
Sbjct: 302 AKDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAASDAIT 361

Query: 72  KRVGEVKKETLKDAMAETTVWKEFVERGTM----LCAVGSPRLGAYDTSFGWGRPIKTEV 131
            R   +K     D +     W + V +  M        G+P+LG YD  FG G+P K E+
Sbjct: 362 AR---IKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEI 421

Query: 132 VQVDRGRLFSIGESRNENGGIEVGLGL 153
           V ++ G   +  ESR+ + G+E+G+ L
Sbjct: 422 VHIETGGSIAFSESRDGSNGVEIGIAL 444

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.8e-17
Identity = 52/146 (35.62%), Postives = 76/146 (52.05%), Query Frame = 0


Query: 13  EDEACCFAFTANCRNERLGYR--IPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEK 72
           +DE        +CRN RL Y   IP  YFGNC A  + +V+K +LLGE  ++ A  AI  
Sbjct: 307 KDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAITA 366

Query: 73  RVGEVKKETLKDAMAETTVWKEFVERGTM----LCAVGSPRLGAYDTSFGWGRPIKTEVV 132
           R   +K     D +     W + V +  M        G+P+LG YD  FG G+P K E+V
Sbjct: 367 R---IKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIV 426

Query: 133 QVDRGRLFSIGESRNENGGIEVGLGL 153
            ++ G   +  ESR+ + G+E+G+ L
Sbjct: 427 HIETGGSIAFSESRDGSNGVEIGIAL 448

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 51/148 (34.46%), Postives = 81/148 (54.73%), Query Frame = 0


Query: 11  GAEDEACCFAFTANCRNERLGYRIPAEYFGNCF--AFCLTTVEKSELLGENGLVVAMKAI 70
           G +D +    F  + R +RL  ++P  YFGNC     C    + +E + E G V A + I
Sbjct: 291 GNKDRSVSLLFVGDFR-DRLDPKLPGTYFGNCMIPVGCYNR-KAAEFMEEKGFVTAAEII 350

Query: 71  E---KRVGEVKKETLKDAMAETTVWKEF-VERGTMLCAVGSPRLGAYDTSFGWGRPIKTE 130
               K +   K ET+ D   E   ++ +  + GT+    GS RLG Y+  FGWGRP+K +
Sbjct: 351 SDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTI---AGSTRLGVYEADFGWGRPVKVD 410

Query: 131 VVQVDRGRLFSIGESRNENGGIEVGLGL 153
           +V +D+G   ++ E R+E+GG+E+G+ L
Sbjct: 411 IVSIDQGEAIAMAERRDESGGVEIGMCL 433

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.1e-14
Identity = 48/150 (32.00%), Postives = 72/150 (48.00%), Query Frame = 0


Query: 5   LISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCL-TTVEKSELLGENGLVV 64
           LI    G       + F  +CR+  +   +P+ YFGNC + C   ++     + E G + 
Sbjct: 304 LIKARGGDPSRPVGYGFAVDCRSLMVP-PVPSSYFGNCVSACFKMSLTAETFMSEEGFLA 363

Query: 65  AMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAV-GSPRLGAYDTSFGWGRPIK 124
           A + +   V E   E +   + E       +  GT + +V GS R G Y   FGWGRP K
Sbjct: 364 AARMVSDSV-EALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEK 423

Query: 125 TEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
             VV +D+G   S  ESR+ +GG+E+G  L
Sbjct: 424 VVVVSIDQGEAISFAESRDGSGGVELGFSL 451

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BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL
Match: A0A6J1JDF0 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484014 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.7e-82
Identity = 152/152 (100.00%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 60
           MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG
Sbjct: 124 MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 183

Query: 61  LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 120
           LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP
Sbjct: 184 LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 243

Query: 121 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
           IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL
Sbjct: 244 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 275

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL
Match: A0A6J1CIE1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111011225 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 7.2e-46
Identity = 91/145 (62.76%), Postives = 107/145 (73.79%), Query Frame = 0


Query: 8   GGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKA 67
           GG G   E CCFAF A+CR +RLGY +PA YFGNC AFC   VEK ELLG +G++V MKA
Sbjct: 217 GGNGKGSEVCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKA 276

Query: 68  IEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQ 127
             KRVGE + E LK+     +VWK++ ERG +    GSPRLGAYD  FGWGRPIKT+V+ 
Sbjct: 277 TGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIH 336

Query: 128 VDRGRLFSIGESRNENGGIEVGLGL 153
           +D G LFS+GESRNENGGIEVGL L
Sbjct: 337 IDSGSLFSLGESRNENGGIEVGLAL 360

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL
Match: A0A061E3T5 (HXXXD-type acyl-transferase family protein, putative OS=Theobroma cacao OX=3641 GN=TCM_007906 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.7e-31
Identity = 74/151 (49.01%), Postives = 97/151 (64.24%), Query Frame = 0


Query: 2   SRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGL 61
           ++EL   G   +DE   F F A+CRN R  + IP  Y GNC + C   +++ ELLGENG+
Sbjct: 298 AQELCGRGKHEDDEFHYFCFVADCRN-RPEFAIPETYLGNCLSICFVRMKRRELLGENGV 357

Query: 62  VVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPI 121
           V A KAI KRV E +K  L++A    T WKE  E G ++   GSP+L AY+T FGWG+P 
Sbjct: 358 VAATKAIGKRVWEFEKGALREAEKWLTNWKEISETGHLVTISGSPKLRAYETDFGWGKPK 417

Query: 122 KTEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
           KTEVVQ+D      +G+SR+E GGIE+GL L
Sbjct: 418 KTEVVQIDVSGAIYLGDSRDEEGGIEIGLAL 447

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL
Match: A0A1Q3B9H9 (Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08224 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 2.3e-31
Identity = 70/139 (50.36%), Postives = 93/139 (66.91%), Query Frame = 0


Query: 14  DEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEKRVG 73
           DE C F F A+C N RL + IPA YFGNC A C+ T++ S+++GE+G+V A KAI  +V 
Sbjct: 326 DELCYFCFVADCCN-RLDFSIPATYFGNCLAICVVTLKMSDIMGEDGIVAATKAIGNKVR 385

Query: 74  EVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQVDRGRL 133
           E++   L+ A    + WKE  E G ++   GSPRL  Y+T FGWG+PIK+EVV +D  R 
Sbjct: 386 ELESGALRGADKWLSCWKEISEAGHVVTVAGSPRLRVYETDFGWGKPIKSEVVHIDMSRG 445

Query: 134 FSIGESRNENGGIEVGLGL 153
             I ESR+E GGIE+GL L
Sbjct: 446 IYICESRDEEGGIEIGLAL 463

[Close]

BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL
Match: A0A6J1B0L4 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Herrania umbratica OX=108875 GN=LOC110423025 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 5.0e-31
Identity = 73/151 (48.34%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 2   SRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGL 61
           ++EL       +DE   F F A+CRN R  + IP  Y GNC + C   +++SELLGENG+
Sbjct: 298 AQELCGNKKHEDDECHYFCFVADCRN-RPEFAIPDTYLGNCLSICFVRMKRSELLGENGV 357

Query: 62  VVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPI 121
           V A KAI  RV E +K  L++A    T WKE  E G ++   GSP+L AY+T FGWG+P 
Sbjct: 358 VAATKAIGNRVWEFEKGALREAEKWLTNWKEISETGHLVTVSGSPKLRAYETDFGWGKPK 417

Query: 122 KTEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
           KTEVVQ+D      +G+SR+E GGIE+GL L
Sbjct: 418 KTEVVQIDVSGAIYLGDSRDEEGGIEIGLAL 447

[Close]

BLAST of CmaCh20G006570.1 vs. NCBI nr
Match: XP_022986200.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 314.3 bits (804), Expect = 7.6e-82
Identity = 152/152 (100.00%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 60
           MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG
Sbjct: 124 MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 183

Query: 61  LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 120
           LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP
Sbjct: 184 LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 243

Query: 121 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
           IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL
Sbjct: 244 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 275

[Close]

BLAST of CmaCh20G006570.1 vs. NCBI nr
Match: XP_023512859.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.3 bits (734), Expect = 9.9e-74
Identity = 138/152 (90.79%), Postives = 140/152 (92.11%), Query Frame = 0


Query: 1   MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 60
           MSRELISGG GAEDEACCFAF ANCRNERLGYRIP EYFGNCFAFCLT VEKSELLGENG
Sbjct: 286 MSRELISGGSGAEDEACCFAFAANCRNERLGYRIPVEYFGNCFAFCLTAVEKSELLGENG 345

Query: 61  LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 120
           LVVAMKAIEKRVGEV KETLKD MAE + WKEFVERG  LCA GSPRLGAYD +FGWGRP
Sbjct: 346 LVVAMKAIEKRVGEVNKETLKDVMAEMSAWKEFVERGAALCAAGSPRLGAYDANFGWGRP 405

Query: 121 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
           IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL
Sbjct: 406 IKTEVVQVDRGRLFSIGESRNENGGIEVGLGL 437

[Close]

BLAST of CmaCh20G006570.1 vs. NCBI nr
Match: KAG7010704.1 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.3 bits (599), Expect = 4.5e-58
Identity = 112/124 (90.32%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 1   MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 60
           MSRELISGG GAEDEACCFAF ANCRNERLGYRIPAEYFGNCFAFCLT VEKSELLGENG
Sbjct: 149 MSRELISGGSGAEDEACCFAFAANCRNERLGYRIPAEYFGNCFAFCLTAVEKSELLGENG 208

Query: 61  LVVAMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRP 120
           LVVAMKAIEKRVGEVKK TLKD MAE +VWKE VERG  LCA GSPRLGAYDT+FGWGRP
Sbjct: 209 LVVAMKAIEKRVGEVKKVTLKDVMAEMSVWKELVERGAALCAAGSPRLGAYDTNFGWGRP 268

Query: 121 IKTE 125
           IKTE
Sbjct: 269 IKTE 272

[Close]

BLAST of CmaCh20G006570.1 vs. NCBI nr
Match: XP_022140618.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Momordica charantia])

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-45
Identity = 91/145 (62.76%), Postives = 107/145 (73.79%), Query Frame = 0


Query: 8   GGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKA 67
           GG G   E CCFAF A+CR +RLGY +PA YFGNC AFC   VEK ELLG +G++V MKA
Sbjct: 217 GGNGKGSEVCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKA 276

Query: 68  IEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQ 127
             KRVGE + E LK+     +VWK++ ERG +    GSPRLGAYD  FGWGRPIKT+V+ 
Sbjct: 277 TGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIH 336

Query: 128 VDRGRLFSIGESRNENGGIEVGLGL 153
           +D G LFS+GESRNENGGIEVGL L
Sbjct: 337 IDSGSLFSLGESRNENGGIEVGLAL 360

[Close]

BLAST of CmaCh20G006570.1 vs. NCBI nr
Match: KAG6570854.1 (Diacylglycerol kinase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-34
Identity = 80/89 (89.89%), Postives = 81/89 (91.01%), Query Frame = 0


Query: 1   MSRELISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENG 60
           MSRELISGG GAEDEACC     NCRNERLGYRIPAEYFGNCFAFCLT VEKSELLGENG
Sbjct: 289 MSRELISGGSGAEDEACC-----NCRNERLGYRIPAEYFGNCFAFCLTAVEKSELLGENG 348

Query: 61  LVVAMKAIEKRVGEVKKETLKDAMAETTV 90
           LVVAMKAIEKRVGEVKK TLKDAMAET+V
Sbjct: 349 LVVAMKAIEKRVGEVKKVTLKDAMAETSV 372

[Close]

BLAST of CmaCh20G006570.1 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.2e-18
Identity = 53/147 (36.05%), Postives = 77/147 (52.38%), Query Frame = 0


Query: 12  AEDEACCFAFTANCRNERLGYR--IPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIE 71
           A+DE        +CRN RL Y   IP  YFGNC A  + +V+K +LLGE  ++ A  AI 
Sbjct: 302 AKDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAASDAIT 361

Query: 72  KRVGEVKKETLKDAMAETTVWKEFVERGTM----LCAVGSPRLGAYDTSFGWGRPIKTEV 131
            R   +K     D +     W + V +  M        G+P+LG YD  FG G+P K E+
Sbjct: 362 AR---IKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEI 421

Query: 132 VQVDRGRLFSIGESRNENGGIEVGLGL 153
           V ++ G   +  ESR+ + G+E+G+ L
Sbjct: 422 VHIETGGSIAFSESRDGSNGVEIGIAL 444

[Close]

BLAST of CmaCh20G006570.1 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.8e-18
Identity = 52/146 (35.62%), Postives = 76/146 (52.05%), Query Frame = 0


Query: 13  EDEACCFAFTANCRNERLGYR--IPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEK 72
           +DE        +CRN RL Y   IP  YFGNC A  + +V+K +LLGE  ++ A  AI  
Sbjct: 307 KDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAITA 366

Query: 73  RVGEVKKETLKDAMAETTVWKEFVERGTM----LCAVGSPRLGAYDTSFGWGRPIKTEVV 132
           R   +K     D +     W + V +  M        G+P+LG YD  FG G+P K E+V
Sbjct: 367 R---IKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIV 426

Query: 133 QVDRGRLFSIGESRNENGGIEVGLGL 153
            ++ G   +  ESR+ + G+E+G+ L
Sbjct: 427 HIETGGSIAFSESRDGSNGVEIGIAL 448

[Close]

BLAST of CmaCh20G006570.1 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.2e-18
Identity = 51/148 (34.46%), Postives = 81/148 (54.73%), Query Frame = 0


Query: 11  GAEDEACCFAFTANCRNERLGYRIPAEYFGNCF--AFCLTTVEKSELLGENGLVVAMKAI 70
           G +D +    F  + R +RL  ++P  YFGNC     C    + +E + E G V A + I
Sbjct: 291 GNKDRSVSLLFVGDFR-DRLDPKLPGTYFGNCMIPVGCYNR-KAAEFMEEKGFVTAAEII 350

Query: 71  E---KRVGEVKKETLKDAMAETTVWKEF-VERGTMLCAVGSPRLGAYDTSFGWGRPIKTE 130
               K +   K ET+ D   E   ++ +  + GT+    GS RLG Y+  FGWGRP+K +
Sbjct: 351 SDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTI---AGSTRLGVYEADFGWGRPVKVD 410

Query: 131 VVQVDRGRLFSIGESRNENGGIEVGLGL 153
           +V +D+G   ++ E R+E+GG+E+G+ L
Sbjct: 411 IVSIDQGEAIAMAERRDESGGVEIGMCL 433

[Close]

BLAST of CmaCh20G006570.1 vs. TAIR 10
Match: AT3G29635.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 82.4 bits (202), Expect = 4.5e-16
Identity = 51/147 (34.69%), Postives = 80/147 (54.42%), Query Frame = 0


Query: 11  GAEDEACCFAFTANCRNERLGYRIPAEYFGNC-FAFCLTTVEKSELLGENGLVVAMKAIE 70
           G E+    F + A+ RN RL   +P  YFGNC F       + +  LGE+G V  ++ + 
Sbjct: 296 GDENRPVRFMYAADFRN-RLDPPVPEMYFGNCVFPIGCFGYKANVFLGEDGFVNMVEILS 355

Query: 71  ---KRVGEVKKETLKDAMAETTVWKEFVERGTMLCAV-GSPRLGAYDTSFGWGRPIKTEV 130
              + +G  K ET+ +     T   + V+ GT + ++ GS + G Y + FGWG+P  +E+
Sbjct: 356 DSVRSIGLRKLETICELYINGT---KSVKPGTQIGSIAGSNQFGLYGSDFGWGKPCNSEI 415

Query: 131 VQVDRGRLFSIGESRNENGGIEVGLGL 153
             +DR   FS+ E R+E GG+E+GL L
Sbjct: 416 ASIDRNEAFSMSERRDEPGGVEIGLCL 438

[Close]

BLAST of CmaCh20G006570.1 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.9e-15
Identity = 48/150 (32.00%), Postives = 72/150 (48.00%), Query Frame = 0


Query: 5   LISGGCGAEDEACCFAFTANCRNERLGYRIPAEYFGNCFAFCL-TTVEKSELLGENGLVV 64
           LI    G       + F  +CR+  +   +P+ YFGNC + C   ++     + E G + 
Sbjct: 304 LIKARGGDPSRPVGYGFAVDCRSLMVP-PVPSSYFGNCVSACFKMSLTAETFMSEEGFLA 363

Query: 65  AMKAIEKRVGEVKKETLKDAMAETTVWKEFVERGTMLCAV-GSPRLGAYDTSFGWGRPIK 124
           A + +   V E   E +   + E       +  GT + +V GS R G Y   FGWGRP K
Sbjct: 364 AARMVSDSV-EALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEK 423

Query: 125 TEVVQVDRGRLFSIGESRNENGGIEVGLGL 153
             VV +D+G   S  ESR+ +GG+E+G  L
Sbjct: 424 VVVVSIDQGEAISFAESRDGSGGVELGFSL 451

The following BLAST results are available for this feature:

BLAST of CmaCh20G006570.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZWR8	1.0e-17	34.97	Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1	[more]
Q9LR73	3.0e-17	36.05	Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabid...	[more]
Q9ZWB4	6.8e-17	35.62	Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabid...	[more]
Q9LRQ8	1.2e-16	34.46	Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2...	[more]
Q940Z5	4.1e-14	32.00	Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1...	[more]

BLAST of CmaCh20G006570.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDF0	3.7e-82	100.00	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=C...	[more]
A0A6J1CIE1	7.2e-46	62.76	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=M...	[more]
A0A061E3T5	1.7e-31	49.01	HXXXD-type acyl-transferase family protein, putative OS=Theobroma cacao OX=3641 ...	[more]
A0A1Q3B9H9	2.3e-31	50.36	Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3...	[more]
A0A6J1B0L4	5.0e-31	48.34	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=H...	[more]

BLAST of CmaCh20G006570.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986200.1	7.6e-82	100.00	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Cuc...	[more]
XP_023512859.1	9.9e-74	90.79	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc...	[more]
KAG7010704.1	4.5e-58	90.32	Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2, partial ...	[more]
XP_022140618.1	1.5e-45	62.76	coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Mom...	[more]
KAG6570854.1	1.2e-34	89.89	Diacylglycerol kinase 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh20G006570.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03495.1	2.2e-18	36.05	HXXXD-type acyl-transferase family protein	[more]
AT1G03940.1	4.8e-18	35.62	HXXXD-type acyl-transferase family protein	[more]
AT3G29670.1	8.2e-18	34.46	HXXXD-type acyl-transferase family protein	[more]
AT3G29635.1	4.5e-16	34.69	HXXXD-type acyl-transferase family protein	[more]
AT5G39050.1	2.9e-15	32.00	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 2..169 e-value: 3.1E-28 score: 100.4
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 18..161 e-value: 6.0E-11 score: 41.6
None	No IPR available	PANTHER	PTHR31625:SF52	COUMAROYL-COA:ANTHOCYANIDIN 3-O-GLUCOSIDE-6''-O-COUMAROYLTRANSFERASE 1-RELATED	coord: 12..160
None	No IPR available	PANTHER	PTHR31625	FAMILY NOT NAMED	coord: 12..160

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh20G006570	CmaCh20G006570	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G006570.1:exon:2213	CmaCh20G006570.1:exon:2213	exon
CmaCh20G006570.1:exon:2212	CmaCh20G006570.1:exon:2212	exon
CmaCh20G006570.1:exon:2211	CmaCh20G006570.1:exon:2211	exon
CmaCh20G006570.1:exon:2210	CmaCh20G006570.1:exon:2210	exon
CmaCh20G006570.1:exon:2209	CmaCh20G006570.1:exon:2209	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G006570.1:cds	CmaCh20G006570.1:cds	CDS
CmaCh20G006570.1:cds	CmaCh20G006570.1:cds_2	CDS
CmaCh20G006570.1:cds	CmaCh20G006570.1:cds_3	CDS
CmaCh20G006570.1:cds	CmaCh20G006570.1:cds_4	CDS
CmaCh20G006570.1:cds	CmaCh20G006570.1:cds_5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh20G006570.1	CmaCh20G006570.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups