CmaCh17G004890.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G004890.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme 1-like
LocationCma_Chr17: 3040257 .. 3042467 (-)
Sequence length1737
RNA-Seq ExpressionCmaCh17G004890.1
SyntenyCmaCh17G004890.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCATTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCGTCACTGGATGAACGGTTCGTAATTTCTACATTTTCATTCCCTCCTTTCCTCCGGTTGTTATTAATGTATTTCCTCCGGTTCATTCCCTCCATAAACATGTCATCTTATGCAGGCCCGAATGGCCCAATGTATTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCAGTTTGGGGGAACATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAACCGGATAACAATCCGATTGTTGACCCGGGTACAGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCATGGCTAAGCCTCAACGGTCACTGGAACACCATCATTGGCAGCAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGAATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTATCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGGTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGAAATTTCTATGCTTCCAAGTCATTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGTTTGTTTTAGATGCAATGTGCTCTGTTTTGTTCTATTTTTAATTGAATTTGCTCTATTTTGTATTTGGGCCGATCTTTTCTTTCTCCTTTTCCTAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAACAGCTCTTACAATGGCCAATTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGCTAAATCAAAAGCTGGTTAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGTTGTTTAATTTATAACGAATCCCCGATAGTGGTTTGTAGCGGGTTAAGAAGATGTGAAAGCACCTCTAAGATTAAGGGAACAAATTGGGCGTCATTTGATCGATTGTTTCTTGTGTTTTATACAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGCTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTGGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGGTAGCTTCAATTCCCTTATCAAAGGACCTTATAATTATTTTTTTTATATTTACAAAACTCTGGTTTCTCTCTTTTGGTGTAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCGAGTAAAAAGCTCTCATTAAGAAGCTTGGTACGTTAAAGTCATAGTCCAATACATTCTTGCACCGTATCGACAAATCTAAACTATGTATTATTGTGCAGATTGATCACTCGGTGATAGAGAGCTTTGGTGGGGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTTGGGTGAACTAA

mRNA sequence

ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCATTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCGTCACTGGATGAACGGCCCGAATGGCCCAATGTATTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCAGTTTGGGGGAACATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAACCGGATAACAATCCGATTGTTGACCCGGGTACAGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCATGGCTAAGCCTCAACGGTCACTGGAACACCATCATTGGCAGCAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGAATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTATCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGGTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGAAATTTCTATGCTTCCAAGTCATTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAACAGCTCTTACAATGGCCAATTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGCTAAATCAAAAGCTGGTTAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGCTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTGGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCGAGTAAAAAGCTCTCATTAAGAAGCTTGATTGATCACTCGGTGATAGAGAGCTTTGGTGGGGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTTGGGTGAACTAA

Coding sequence (CDS)

ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCATTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCGTCACTGGATGAACGGCCCGAATGGCCCAATGTATTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCAGTTTGGGGGAACATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAACCGGATAACAATCCGATTGTTGACCCGGGTACAGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCATGGCTAAGCCTCAACGGTCACTGGAACACCATCATTGGCAGCAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGAATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTATCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGGTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGAAATTTCTATGCTTCCAAGTCATTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAACAGCTCTTACAATGGCCAATTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGCTAAATCAAAAGCTGGTTAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGCTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTGGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCGAGTAAAAAGCTCTCATTAAGAAGCTTGATTGATCACTCGGTGATAGAGAGCTTTGGTGGGGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTTGGGTGAACTAA

Protein sequence

MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN
Homology
BLAST of CmaCh17G004890.1 vs. ExPASy Swiss-Prot
Match: P26792 (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 PE=1 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.2e-229
Identity = 375/554 (67.69%), Postives = 449/554 (81.05%), Query Frame = 0

Query: 27  IQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYN 86
           ++A H+I+  LQS+   N    +KQ+HRTG+HFQP ++W+N PNGPMY+KG+YHLFYQYN
Sbjct: 37  VEAFHEIHYNLQSVGAEN----VKQVHRTGYHFQPKQNWINDPNGPMYYKGVYHLFYQYN 96

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
           P+GAVWGNIVWAHS+S DLINW  L+PA+ PSKPFD  GC SGSAT+LPG KPVILYTGI
Sbjct: 97  PKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFDKYGCRSGSATILPGNKPVILYTGI 156

Query: 147 ---DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGH 206
                +N QVQNYAIPANLSDP+L +W+KPDNNP+V      N++AFRDP+TAWL  +GH
Sbjct: 157 VEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLVVANNGENATAFRDPTTAWLDKSGH 216

Query: 207 WNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDT 266
           W  ++GS+  + G+AYLYRS++F+ WTK + P+HS  NTGMWECPDF+PVS  G  GLDT
Sbjct: 217 WKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQANTGMWECPDFFPVSLKGLNGLDT 276

Query: 267 SVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKS 326
           SV G   KHVLKVSL++TRYEYYTVG Y    D+Y+PDNTS DGW+GLRYDYGNFYASK+
Sbjct: 277 SVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYIPDNTSVDGWAGLRYDYGNFYASKT 336

Query: 327 FYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLR 386
           F+DP K RR+LWGWANESDS   DV+KGWAGIQ IPRT+WLDP+GKQL+QWPI EL  LR
Sbjct: 337 FFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIPRTLWLDPSGKQLMQWPIEELETLR 396

Query: 387 -RKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV--DAQAVCSQ 446
             K+     Q L KG  VE+KGITAAQADVEV FSF SL   E FDP W+  DA+ +CS 
Sbjct: 397 GSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSFKSLAKREPFDPKWLEYDAEKICSL 456

Query: 447 LGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHVVLMCSDARSSSLKDGLYKPT 506
            GST QGGVGPFGLLTLASE LEE+TPVFFR+FKA+  H VLMCSDA  SSLK+GLY+P+
Sbjct: 457 KGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKAQNTHKVLMCSDATRSSLKEGLYRPS 516

Query: 507 FAGFVDIDLSS-KKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHVFNNGT 566
           FAGFVD+DL++ KK+SLRSLID+SV+ESFG  GKTCI+SRVYPT AV+ +AHL+VFNNG+
Sbjct: 517 FAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKTCISSRVYPTLAVYENAHLYVFNNGS 576

Query: 567 ESITVEYLSAWSMR 574
           E+ITVE L AWSM+
Sbjct: 577 ETITVENLDAWSMK 586

BLAST of CmaCh17G004890.1 vs. ExPASy Swiss-Prot
Match: Q39693 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 7.4e-226
Identity = 378/574 (65.85%), Postives = 449/574 (78.22%), Query Frame = 0

Query: 7   LAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWM 66
           L +L  FSF  L I NG   + + H+++PELQS+       ++K +HRTG+HFQP +HW+
Sbjct: 15  LFLLSIFSFIFLNI-NG---VDSTHRVFPELQSI----SAVDVKLVHRTGYHFQPQKHWI 74

Query: 67  NGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGC 126
           N PNGPM++KG YHLFYQYNP+G+VWGNIVWAHS+SKDLINW +L+PA+ PSKPFD  GC
Sbjct: 75  NDPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFDQYGC 134

Query: 127 WSGSATVLPGLKPVILYTGI---DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGT 186
           WSGSAT+LPG KPVILYTGI   D +N QVQNYA+PAN SDP L EWVKPDNNP+V   T
Sbjct: 135 WSGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLVGVHT 194

Query: 187 EVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTG 246
           E N SAFRDP+TAW    GHW  ++GS  +  G+AYLYRS++F  W +   P+H+   TG
Sbjct: 195 E-NPSAFRDPTTAWFD-GGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTKAETG 254

Query: 247 MWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNT 306
           MWECPDFYPVS     GLD S MG   KHVLKVSL  TRYEYYT+G+Y  + D YVPDNT
Sbjct: 255 MWECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYVPDNT 314

Query: 307 SADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVW 366
           S DGW+GLRYDYGNFYASK+FYDP+KKRR+LWGWANESDS  DDV KGWAGIQ IPR +W
Sbjct: 315 SVDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIPRRIW 374

Query: 367 LDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDN 426
           LDP+G+QL+QWPI E+  LR   + M NQKL  G +VE+ GITAAQADV+  FSF SLD 
Sbjct: 375 LDPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFKSLDK 434

Query: 427 AEAFDPSWV--DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV- 486
           AE+FDP W+  DAQ VC  +GST QGG+GPFGLLTLAS++LEE+TPVFFRIFKAE   + 
Sbjct: 435 AESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAEDQKLK 494

Query: 487 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 546
           VLMCSDA+ SSL +GLYKP+F GFVD+DLS KK+SLRSLID+SV+ESFG   K  I+SRV
Sbjct: 495 VLMCSDAKRSSLAEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNLISSRV 554

Query: 547 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRS 575
           YPT A++ +AHL VFNNGTE ITV+ L AWSM S
Sbjct: 555 YPTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of CmaCh17G004890.1 vs. ExPASy Swiss-Prot
Match: Q39692 (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 8.2e-225
Identity = 378/575 (65.74%), Postives = 453/575 (78.78%), Query Frame = 0

Query: 6   LLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHW 65
           L A++F FS      G     ++A H+++PELQS+   N    ++ +HRTG+HFQP +HW
Sbjct: 22  LSAVVFIFSLDVNIRG-----VEASHQVFPELQSVSAVN----VQLVHRTGYHFQPKKHW 81

Query: 66  MNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 125
           +N PNGPMY+KG YHLFYQYNP+GAVWGNIVWAHSISKDLINW +L+PA+ PSKPFD  G
Sbjct: 82  INDPNGPMYYKGFYHLFYQYNPKGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDKYG 141

Query: 126 CWSGSATVLPGLKPVILYTGIDTQ---NRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG 185
           CWSGSATVLPG KPVI+YTGI T    N QVQN+A+PAN SDP+L EW+KPDNNPIV   
Sbjct: 142 CWSGSATVLPGGKPVIMYTGIVTPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPIVRAR 201

Query: 186 TEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNT 245
           +E NSS+FRDP+TAW     HW  ++GSR +  G+AYLYRSRNFL WTK + PLHS   T
Sbjct: 202 SE-NSSSFRDPTTAWFD-GVHWKILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKDRT 261

Query: 246 GMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDN 305
           GMWEC DFYPV+  G  GLDTSV G   KHVLKVSL  TRYEYYTVG+Y    D YVPDN
Sbjct: 262 GMWECLDFYPVAPKGMNGLDTSVTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVPDN 321

Query: 306 TSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTV 365
           TS DGW+GLRYDYGNFYASK+F+DP K+RR+LWGWANESDS QDDV KGWAGIQ IPR +
Sbjct: 322 TSVDGWAGLRYDYGNFYASKTFFDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPRKL 381

Query: 366 WLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLD 425
           WLDPNGKQL+QWPI E+  LR + V M +Q L  G+++E+KG+TAAQADV+  FSF SLD
Sbjct: 382 WLDPNGKQLIQWPIEEIQLLRGQNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKSLD 441

Query: 426 NAEAFDPSW--VDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 485
            AE FDP+W  +DA  VC  +GST +GG+GPFG LTLASE LEE+TPVFFR+FK +    
Sbjct: 442 RAEWFDPNWAKLDALDVCDWMGSTVRGGLGPFGFLTLASEKLEEYTPVFFRVFKTKDKLK 501

Query: 486 VLMCSDA-RSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 545
           VLMCSDA RSS+  +GLYKP FAG+VD+DLS KK+SLRSLID+SV+ESFG  G+TCITSR
Sbjct: 502 VLMCSDAKRSSTTAEGLYKPPFAGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCITSR 561

Query: 546 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRS 575
           VYP  A++ +AH+ VFNNGTE+IT++ L AWSM++
Sbjct: 562 VYPKIAIYNNAHVFVFNNGTEAITIDSLDAWSMKA 585

BLAST of CmaCh17G004890.1 vs. ExPASy Swiss-Prot
Match: Q8W413 (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3702 GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 334/558 (59.86%), Postives = 423/558 (75.81%), Query Frame = 0

Query: 27  IQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYN 86
           I A H+IY ELQS         +  LHR  FHFQP +HW+N PNGP+Y+KG+YHLFYQYN
Sbjct: 27  IDAFHQIYEELQS----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQYN 86

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
            +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG+
Sbjct: 87  TKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTGV 146

Query: 147 DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWNT 206
           +    Q+QNYAIP + SDP+L +W+KPD+NPI  P   +N SAFRDP+TAW S +GHW T
Sbjct: 147 NQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWRT 206

Query: 207 IIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTSV 266
           ++GS+ ++ G+AY+YRSR+F +W K + P+HS  +TGMWECPDF+PVS    R GLD   
Sbjct: 207 VVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLDY 266

Query: 267 MGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFY 326
           +G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F+
Sbjct: 267 VGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTFF 326

Query: 327 DPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRK 386
           D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WP+ E+  LR  
Sbjct: 327 DYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRGN 386

Query: 387 IVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV-DAQAVCSQLGST 446
            V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS 
Sbjct: 387 YVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGSN 446

Query: 447 AQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------KD 506
            +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        KD
Sbjct: 447 VRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAKD 506

Query: 507 GLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHV 566
            +YKP+FAGFVD+D++  ++SLRSLIDHSV+ESFG  GKT ITSRVYP KAV  +AHL+V
Sbjct: 507 RMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLYV 566

Query: 567 FNNGTESITVEYLSAWSM 573
           FNNGT+++T+E L+AW+M
Sbjct: 567 FNNGTQTVTIESLNAWNM 580

BLAST of CmaCh17G004890.1 vs. ExPASy Swiss-Prot
Match: Q1PEF8 (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3702 GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.7e-199
Identity = 333/560 (59.46%), Postives = 416/560 (74.29%), Query Frame = 0

Query: 27  IQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYN 86
           + A HK++ +LQS         +  LHRT +HFQP RHW+N PN PM +KG+YHLFYQYN
Sbjct: 23  VDAFHKVFKKLQS--KSTSLESVSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQYN 82

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
           P+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTGI
Sbjct: 83  PKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGI 142

Query: 147 DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLS-LNGHWN 206
                Q+QNYAIP +LSDP+L  W+KPD+NPIV P    N SAFRDP+TAW +  +G+W 
Sbjct: 143 TENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYWR 202

Query: 207 TIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTS 266
            ++GS+ +  G+AY+Y+SR+F  W K ++P+HS   TGMWECPDF+PVS    + GLD S
Sbjct: 203 MLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDFS 262

Query: 267 VMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 326
             G   KHVLKVSL++TRYEYYT+G Y    D+Y PD  + DGW GLR+DYGN+YASK+F
Sbjct: 263 YDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKTF 322

Query: 327 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRR 386
           +D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQL+ WPI E+  LR 
Sbjct: 323 FDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLRG 382

Query: 387 KIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQ-AVCSQLGS 446
           K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S+      +C+  GS
Sbjct: 383 KNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKGS 442

Query: 447 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------K 506
              GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA+ SSL        K
Sbjct: 443 NVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDTGTDAK 502

Query: 507 DGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLH 566
           + +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSRVYPTKAV   AHL 
Sbjct: 503 ERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHLF 562

Query: 567 VFNNGTESITVEYLSAWSMR 574
           VFNNG++ +TVE L+AW+M+
Sbjct: 563 VFNNGSQPVTVESLNAWNMQ 580

BLAST of CmaCh17G004890.1 vs. ExPASy TrEMBL
Match: A0A6J1KY17 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499236 PE=3 SV=1)

HSP 1 Score: 1212.6 bits (3136), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV
Sbjct: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 578

BLAST of CmaCh17G004890.1 vs. ExPASy TrEMBL
Match: A0A6J1KW51 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499237 PE=3 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 570/578 (98.62%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLP LKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPDLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
            VWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQ VEIKGITAAQADVEVIFSFSS
Sbjct: 361 MVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQIVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV
Sbjct: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 578

BLAST of CmaCh17G004890.1 vs. ExPASy TrEMBL
Match: A0A6J1KWI1 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499336 PE=3 SV=1)

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 570/579 (98.45%), Postives = 575/579 (99.31%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60

Query: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA VN
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARVN 579

BLAST of CmaCh17G004890.1 vs. ExPASy TrEMBL
Match: A0A6J1H3Y1 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460204 PE=3 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 551/581 (94.84%), Postives = 560/581 (96.39%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAIL GFSF SLFI NG VV+QALHKIYPELQSLE GNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILLGFSFYSLFIDNGLVVVQALHKIYPELQSLEKGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSIS+DLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPG KPVILYTGID+QNRQVQNYAIPANL DPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLS NGHWNTIIGSRSEQTGMAYLY S++FLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSFNGHWNTIIGSRSEQTGMAYLYTSKDFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFG+DTSV GNW KHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGVDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWP+MELNRLRRK VMM++QKLVKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 361 TVWLDPNGKQLLQWPVMELNRLRRKRVMMIHQKLVKGQNVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA---EA 480
           LD AEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFK    E 
Sbjct: 421 LDKAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNET 480

Query: 481 NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCIT 540
           NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCIT
Sbjct: 481 NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCIT 540

Query: 541 SRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           SRVYPTKAVF DAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 SRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 581

BLAST of CmaCh17G004890.1 vs. ExPASy TrEMBL
Match: A0A6J1H4M5 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460004 PE=3 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 544/580 (93.79%), Postives = 559/580 (96.38%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAIL GFSF SLF+ NGPVV+QALHK+YP+LQSL+NGN+ TEIKQLHRTGFHFQ
Sbjct: 45  MNFFTLLAILLGFSFYSLFVDNGPVVVQALHKLYPKLQSLKNGNN-TEIKQLHRTGFHFQ 104

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSIS+DLINWKSLKPALSPSKP
Sbjct: 105 PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSKP 164

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPG KPVILYTGID+QNRQVQNYAIPANL DPHLTEWVKPDNNPIVD
Sbjct: 165 FDINGCWSGSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIVD 224

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLS NGHWNTIIGSRSEQTGMAYLY S++FLNWTKVEQPLHSVP
Sbjct: 225 PGTEVNSSAFRDPSTAWLSFNGHWNTIIGSRSEQTGMAYLYTSKDFLNWTKVEQPLHSVP 284

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFG+DTSV GNW KHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 285 NTGMWECPDFYPVSHFGRFGVDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 344

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 345 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 404

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWP+MELNRLRRK VMM++QKLVKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 405 TVWLDPNGKQLLQWPVMELNRLRRKRVMMIHQKLVKGQNVEIKGITAAQADVEVIFSFSS 464

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA---EA 480
           LD AEAFDPSWVDAQAVCSQLGS AQGGVGPFGLLTLASENLEEFTPVFFRIFK    E 
Sbjct: 465 LDKAEAFDPSWVDAQAVCSQLGSRAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNET 524

Query: 481 NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCIT 540
           NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCIT
Sbjct: 525 NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCIT 584

Query: 541 SRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWV 578
           SRVYPTKAVF DAHLHVFNNGTESITVEYL+AWSMRSAWV
Sbjct: 585 SRVYPTKAVFEDAHLHVFNNGTESITVEYLNAWSMRSAWV 623

BLAST of CmaCh17G004890.1 vs. NCBI nr
Match: XP_023006546.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1212.6 bits (3136), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV
Sbjct: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 578

BLAST of CmaCh17G004890.1 vs. NCBI nr
Match: XP_023006547.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 570/578 (98.62%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLP LKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPDLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
            VWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQ VEIKGITAAQADVEVIFSFSS
Sbjct: 361 MVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQIVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV
Sbjct: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 578

BLAST of CmaCh17G004890.1 vs. NCBI nr
Match: XP_023006682.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 570/579 (98.45%), Postives = 575/579 (99.31%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60

Query: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA VN
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARVN 579

BLAST of CmaCh17G004890.1 vs. NCBI nr
Match: XP_023547722.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 563/578 (97.40%), Postives = 568/578 (98.27%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAIL GFSFCSLFI NG VV+QALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPGLKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHW TIIGSRSEQTGMAYLYRS++FLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSV GNW KHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWPIMELNRLRRKIVMM+NQKLVKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAE NHV
Sbjct: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVF DAHLHVFNNGTESITVEYLSAWSMRSAWVN
Sbjct: 541 YPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSAWVN 578

BLAST of CmaCh17G004890.1 vs. NCBI nr
Match: XP_023547716.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 554/578 (95.85%), Postives = 564/578 (97.58%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQ 60
           MNFFTLLAIL  FSFCSLF+ NGPVV+QALHK YP+LQSLENGND TEIKQLHRTGFHFQ
Sbjct: 1   MNFFTLLAILLQFSFCSLFVDNGPVVVQALHKFYPKLQSLENGND-TEIKQLHRTGFHFQ 60

Query: 61  PHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120
           PHRHWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP
Sbjct: 61  PHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKP 120

Query: 121 FDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180
           FDINGCWSGSATVLPGLKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD
Sbjct: 121 FDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVD 180

Query: 181 PGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVP 240
           PGTEVNSSAFRDPSTAWLSLNGHW TIIGSRSEQTGMAYLYRS++FLNWTKVEQPLHSVP
Sbjct: 181 PGTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSVP 240

Query: 241 NTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300
           NTGMWECPDFYPVSHFGRFGLDTSV GNW KHVLKVSLEMTRYEYYTVGKYYPMTDKYVP
Sbjct: 241 NTGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYVP 300

Query: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360
           DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR
Sbjct: 301 DNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSS 420
           TVWLDPNGKQLLQWPIMELNRLRRKIV+M+NQK+VKGQNVEIKGITAAQADVEVIFSFSS
Sbjct: 361 TVWLDPNGKQLLQWPIMELNRLRRKIVLMINQKVVKGQNVEIKGITAAQADVEVIFSFSS 420

Query: 421 LDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 480
           LDNAE FDPSWVDAQAVCSQLGST QGGVGPFGLLTLASENLEEFTPVFFRIFKAE NHV
Sbjct: 421 LDNAETFDPSWVDAQAVCSQLGSTTQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNHV 480

Query: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRV 540
           VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCITSRV
Sbjct: 481 VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSRV 540

Query: 541 YPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
           YPTKAVF DAHLHVFNNGTESITV+YLSAWSMRSAWVN
Sbjct: 541 YPTKAVFEDAHLHVFNNGTESITVDYLSAWSMRSAWVN 577

BLAST of CmaCh17G004890.1 vs. TAIR 10
Match: AT2G36190.1 (cell wall invertase 4 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 334/558 (59.86%), Postives = 423/558 (75.81%), Query Frame = 0

Query: 27  IQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYN 86
           I A H+IY ELQS         +  LHR  FHFQP +HW+N PNGP+Y+KG+YHLFYQYN
Sbjct: 27  IDAFHQIYEELQS----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQYN 86

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
            +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG+
Sbjct: 87  TKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTGV 146

Query: 147 DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLSLNGHWNT 206
           +    Q+QNYAIP + SDP+L +W+KPD+NPI  P   +N SAFRDP+TAW S +GHW T
Sbjct: 147 NQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWRT 206

Query: 207 IIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTSV 266
           ++GS+ ++ G+AY+YRSR+F +W K + P+HS  +TGMWECPDF+PVS    R GLD   
Sbjct: 207 VVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLDY 266

Query: 267 MGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFY 326
           +G   KHVLKVSL++TRYEYYT+GKY    D+Y+PD  + DGW GLR+DYGNFYASK+F+
Sbjct: 267 VGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTFF 326

Query: 327 DPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRRK 386
           D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WP+ E+  LR  
Sbjct: 327 DYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRGN 386

Query: 387 IVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV-DAQAVCSQLGST 446
            V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS 
Sbjct: 387 YVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGSN 446

Query: 447 AQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------KD 506
            +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        KD
Sbjct: 447 VRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAKD 506

Query: 507 GLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHV 566
            +YKP+FAGFVD+D++  ++SLRSLIDHSV+ESFG  GKT ITSRVYP KAV  +AHL+V
Sbjct: 507 RMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLYV 566

Query: 567 FNNGTESITVEYLSAWSM 573
           FNNGT+++T+E L+AW+M
Sbjct: 567 FNNGTQTVTIESLNAWNM 580

BLAST of CmaCh17G004890.1 vs. TAIR 10
Match: AT3G52600.1 (cell wall invertase 2 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.9e-200
Identity = 333/560 (59.46%), Postives = 416/560 (74.29%), Query Frame = 0

Query: 27  IQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFKGIYHLFYQYN 86
           + A HK++ +LQS         +  LHRT +HFQP RHW+N PN PM +KG+YHLFYQYN
Sbjct: 23  VDAFHKVFKKLQS--KSTSLESVSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQYN 82

Query: 87  PEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGI 146
           P+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTGI
Sbjct: 83  PKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGI 142

Query: 147 DTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRDPSTAWLS-LNGHWN 206
                Q+QNYAIP +LSDP+L  W+KPD+NPIV P    N SAFRDP+TAW +  +G+W 
Sbjct: 143 TENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYWR 202

Query: 207 TIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTS 266
            ++GS+ +  G+AY+Y+SR+F  W K ++P+HS   TGMWECPDF+PVS    + GLD S
Sbjct: 203 MLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDFS 262

Query: 267 VMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 326
             G   KHVLKVSL++TRYEYYT+G Y    D+Y PD  + DGW GLR+DYGN+YASK+F
Sbjct: 263 YDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKTF 322

Query: 327 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPIMELNRLRR 386
           +D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQL+ WPI E+  LR 
Sbjct: 323 FDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLRG 382

Query: 387 KIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQ-AVCSQLGS 446
           K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S+      +C+  GS
Sbjct: 383 KNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKGS 442

Query: 447 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------K 506
              GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA+ SSL        K
Sbjct: 443 NVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDTGTDAK 502

Query: 507 DGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLH 566
           + +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSRVYPTKAV   AHL 
Sbjct: 503 ERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHLF 562

Query: 567 VFNNGTESITVEYLSAWSMR 574
           VFNNG++ +TVE L+AW+M+
Sbjct: 563 VFNNGSQPVTVESLNAWNMQ 580

BLAST of CmaCh17G004890.1 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 664.5 bits (1713), Expect = 8.0e-191
Identity = 319/571 (55.87%), Postives = 408/571 (71.45%), Query Frame = 0

Query: 17  SLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFK 76
           +L IGN  V ++A H +Y  L    N   +  + Q +RTGFHFQP ++WMN PNGPM +K
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYK 75

Query: 77  GIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPG 136
           GIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 137 LKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVNSSAFRDPS 196
            KPVILYTGID +N+QVQN A P NLSDP+L EW K   NP++ P     +N+S+FRDP+
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 197 TAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVS 256
           TAWL  +  W  IIGS+  + G+A  Y S++FL W K  +PLH    +GMWECPDF+PV+
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 257 HFGRFGLDTSVMG---NWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGL 316
            FG  G++TS  G      KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 317 RYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQL 376
           RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 377 LQWPIMELNRLRRKIVMMLNQKLVK-GQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPS 436
           +QWP+ E+ RLR K V  L  K++K G  +E+ G+TAAQADVEV+F    L+ A+  +PS
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 437 WVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEAN---HVVLMCSDA 496
           W D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 497 RSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVF 556
             SSLK+   K T+  FVDI+   + LSLR+LIDHSV+ESFGG G+ CITSRVYP  A+ 
Sbjct: 496 SRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 555

Query: 557 GDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
             +HL  FN G +S+ V  L+AWSM SA ++
Sbjct: 556 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of CmaCh17G004890.1 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 654.4 bits (1687), Expect = 8.3e-188
Identity = 317/571 (55.52%), Postives = 406/571 (71.10%), Query Frame = 0

Query: 17  SLFIGNGPVVIQALHKIYPELQSLENGNDTTEIKQLHRTGFHFQPHRHWMNGPNGPMYFK 76
           +L IGN  V ++A H +Y  L    N   +  + Q +RTGFHFQP ++WM   NGPM +K
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPPKNWM---NGPMIYK 75

Query: 77  GIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPG 136
           GIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 137 LKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPG--TEVNSSAFRDPS 196
            KPVILYTGID +N+QVQN A P NLSDP+L EW K   NP++ P     +N+S+FRDP+
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 197 TAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFYPVS 256
           TAWL  +  W  IIGS+  + G+A  Y S++FL W K  +PLH    +GMWECPDF+PV+
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 257 HFGRFGLDTSVMG---NWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSGL 316
            FG  G++TS  G      KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 317 RYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQL 376
           RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 377 LQWPIMELNRLRRKIVMMLNQKLVK-GQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPS 436
           +QWP+ E+ RLR K V  L  K++K G  +E+ G+TAAQADVEV+F    L+ A+  +PS
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 437 WVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEAN---HVVLMCSDA 496
           W D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 497 RSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVF 556
             SSLK+   K T+  FVDI+   + LSLR+LIDHSV+ESFGG G+ CITSRVYP  A+ 
Sbjct: 496 SRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIG 555

Query: 557 GDAHLHVFNNGTESITVEYLSAWSMRSAWVN 579
             +HL  FN G +S+ V  L+AWSM SA ++
Sbjct: 556 KSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of CmaCh17G004890.1 vs. TAIR 10
Match: AT3G52600.2 (cell wall invertase 2 )

HSP 1 Score: 647.9 bits (1670), Expect = 7.7e-186
Identity = 312/514 (60.70%), Postives = 388/514 (75.49%), Query Frame = 0

Query: 73  MYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSAT 132
           M +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT
Sbjct: 1   MLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSAT 60

Query: 133 VLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIVDPGTEVNSSAFRD 192
            +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+KPD+NPIV P    N SAFRD
Sbjct: 61  HVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRD 120

Query: 193 PSTAWLS-LNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSVPNTGMWECPDFY 252
           P+TAW +  +G+W  ++GS+ +  G+AY+Y+SR+F  W K ++P+HS   TGMWECPDF+
Sbjct: 121 PTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFF 180

Query: 253 PVSHFG-RFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYVPDNTSADGWSG 312
           PVS    + GLD S  G   KHVLKVSL++TRYEYYT+G Y    D+Y PD  + DGW G
Sbjct: 181 PVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDG 240

Query: 313 LRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQ 372
           LR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQ
Sbjct: 241 LRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQ 300

Query: 373 LLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPS 432
           L+ WPI E+  LR K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S
Sbjct: 301 LVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDES 360

Query: 433 WVDAQ-AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDA 492
           +      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA
Sbjct: 361 FATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDA 420

Query: 493 RSSSL--------KDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 552
           + SSL        K+ +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSR
Sbjct: 421 KPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSR 480

Query: 553 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMR 574
           VYPTKAV   AHL VFNNG++ +TVE L+AW+M+
Sbjct: 481 VYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P267924.2e-22967.69Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 ... [more]
Q396937.4e-22665.85Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 ... [more]
Q396928.2e-22565.74Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 ... [more]
Q8W4132.5e-20559.86Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3... [more]
Q1PEF82.7e-19959.46Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1KY170.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1KW510.0e+0098.62beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1KWI10.0e+0098.45beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1H3Y10.0e+0094.84beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=366... [more]
A0A6J1H4M50.0e+0093.79beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
XP_023006546.10.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
XP_023006547.10.0e+0098.62beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
XP_023006682.10.0e+0098.45beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
XP_023547722.10.0e+0097.40beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo][more]
XP_023547716.10.0e+0095.85beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G36190.11.8e-20659.86cell wall invertase 4 [more]
AT3G52600.11.9e-20059.46cell wall invertase 2 [more]
AT3G13790.18.0e-19155.87Glycosyl hydrolases family 32 protein [more]
AT3G13790.28.3e-18855.52Glycosyl hydrolases family 32 protein [more]
AT3G52600.27.7e-18660.70cell wall invertase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 58..532
e-value: 4.1E-232
score: 787.1
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 62..388
e-value: 6.1E-115
score: 386.1
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 50..376
NoneNo IPR availableGENE3D2.60.120.560coord: 389..572
e-value: 2.0E-78
score: 264.4
NoneNo IPR availablePANTHERPTHR31953:SF65BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME 1-LIKEcoord: 8..576
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 8..576
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 64..364
e-value: 2.31143E-168
score: 478.034
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 378..570
e-value: 1.1E-25
score: 90.5
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 58..375
e-value: 1.4E-102
score: 343.3
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 375..576

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh17G004890CmaCh17G004890gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh17G004890.1:exon:3089CmaCh17G004890.1:exon:3089exon
CmaCh17G004890.1:exon:3090CmaCh17G004890.1:exon:3090exon
CmaCh17G004890.1:exon:3091CmaCh17G004890.1:exon:3091exon
CmaCh17G004890.1:exon:3092CmaCh17G004890.1:exon:3092exon
CmaCh17G004890.1:exon:3093CmaCh17G004890.1:exon:3093exon
CmaCh17G004890.1:exon:3094CmaCh17G004890.1:exon:3094exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh17G004890.1:cdsCmaCh17G004890.1:cds_6CDS
CmaCh17G004890.1:cdsCmaCh17G004890.1:cds_5CDS
CmaCh17G004890.1:cdsCmaCh17G004890.1:cds_4CDS
CmaCh17G004890.1:cdsCmaCh17G004890.1:cds_3CDS
CmaCh17G004890.1:cdsCmaCh17G004890.1:cds_2CDS
CmaCh17G004890.1:cdsCmaCh17G004890.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh17G004890.1CmaCh17G004890.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds