CmaCh12G000480.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G000480.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	vinorine synthase-like
Location	Cma_Chr12: 183218 .. 184531 (-)
Sequence length	1314
RNA-Seq Expression	CmaCh12G000480.1
Synteny	CmaCh12G000480.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTGAAATTGACATCATCTCAGAGGATTTAATCAAACCTGCTACTCCCACGCCTCCCCACCTCCGCCATTACCCCCTTTCCTTCATCGACCAAATCACCGTTCACATTTATGCTCCCGCCCTCTACTTCTACCGACCCACCCACGCCGGAGATCCCGATTTCGCCATCCACTTCCGGAACCGCCTCCGGAGCACTCTCTCCCTCGTCCTCTCCCACTACCCTCCACTCGCCGGCCGCCCCAACTACGACTCCGCCTTTGTGGACTGCAATGACACGGGGGCCTTATTTCGGGAAGCGCGAGTGAATACAAAACTCGCTGACGTGGTGGAATATGCCCAACCGGACGATCTGAACCGCCTGTTCCCGGTCGAGTTGGACCAGTTCAACGAAGAGCTGATGGCGGTTCAGTTCACGGAGTTCGCGTGCGGTGGGGTGGCGGTGGCGTCGTGCATCTCGCATAAGATTGCGGATGCGATGACGCTGTTTTCGCTGAATAATAACTGGGCGGCGATGGCGCGTGGAGTGAAGGGGGTTTTCAAACCGCACATGGAAGGGGCTAAGTTGTTCCCGGCGAAAACGACGAGGTATGATACGGCGAAGACGATTCTGAGAAACAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAGGCGATTCGCGAGAAATACGGAGAGAATGGCGCAGCGCACCGACCTTCACGGGTCGAGTCATTGACGGCTTTTCTGTACGGACGGTTTCTTGCGGCTCTTAAAGAGGGCTCTTCTTCTGAAGTTCCGAGCGACAGGGCATGTCTAGTGAACTACACAGTGAATCTGCGGCCAAAAATGGAGCCGCCACTTCCGGGCGACGCCTTTGGGAACTACTATTTCAACGCCATGATCTTCCCGTCCCCGGACATTCTGAACGACCCGTACTGCCACGGTCTAGTGAACCGGCTGAGAGAAGAAACCAACAAGCTGAACGCCCAGACAGTGAAGTACATTCTGAATGAAGACCAGAGGCTGATGGAGAGCGTGGAGGAGATTGCTTCTAAGTTCGTGAAGGGAGAGATGATTTCGTGTGCGTTCACTAGCATCTGCCGGTTTCCCCTTTATGACGTGGATTTCGGGTGGGGCCGGCCTGTGTGGGTGACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGACGTTGTTGGATTCCAAAGAGGCCGGCGGCATTGATGCGATTGTTCACCTTGAGGAACCCCACATGAAAAGGCTTGAAGCTGATCAACTCTTCCTTGAGTATGCTACTCCAACTCCTACTGCCCCGGCCACCCCATAA

mRNA sequence

ATGAAGCTTGAAATTGACATCATCTCAGAGGATTTAATCAAACCTGCTACTCCCACGCCTCCCCACCTCCGCCATTACCCCCTTTCCTTCATCGACCAAATCACCGTTCACATTTATGCTCCCGCCCTCTACTTCTACCGACCCACCCACGCCGGAGATCCCGATTTCGCCATCCACTTCCGGAACCGCCTCCGGAGCACTCTCTCCCTCGTCCTCTCCCACTACCCTCCACTCGCCGGCCGCCCCAACTACGACTCCGCCTTTGTGGACTGCAATGACACGGGGGCCTTATTTCGGGAAGCGCGAGTGAATACAAAACTCGCTGACGTGGTGGAATATGCCCAACCGGACGATCTGAACCGCCTGTTCCCGGTCGAGTTGGACCAGTTCAACGAAGAGCTGATGGCGGTTCAGTTCACGGAGTTCGCGTGCGGTGGGGTGGCGGTGGCGTCGTGCATCTCGCATAAGATTGCGGATGCGATGACGCTGTTTTCGCTGAATAATAACTGGGCGGCGATGGCGCGTGGAGTGAAGGGGGTTTTCAAACCGCACATGGAAGGGGCTAAGTTGTTCCCGGCGAAAACGACGAGGTATGATACGGCGAAGACGATTCTGAGAAACAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAGGCGATTCGCGAGAAATACGGAGAGAATGGCGCAGCGCACCGACCTTCACGGGTCGAGTCATTGACGGCTTTTCTGTACGGACGGTTTCTTGCGGCTCTTAAAGAGGGCTCTTCTTCTGAAGTTCCGAGCGACAGGGCATGTCTAGTGAACTACACAGTGAATCTGCGGCCAAAAATGGAGCCGCCACTTCCGGGCGACGCCTTTGGGAACTACTATTTCAACGCCATGATCTTCCCGTCCCCGGACATTCTGAACGACCCGTACTGCCACGGTCTAGTGAACCGGCTGAGAGAAGAAACCAACAAGCTGAACGCCCAGACAGTGAAGTACATTCTGAATGAAGACCAGAGGCTGATGGAGAGCGTGGAGGAGATTGCTTCTAAGTTCGTGAAGGGAGAGATGATTTCGTGTGCGTTCACTAGCATCTGCCGGTTTCCCCTTTATGACGTGGATTTCGGGTGGGGCCGGCCTGTGTGGGTGACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGACGTTGTTGGATTCCAAAGAGGCCGGCGGCATTGATGCGATTGTTCACCTTGAGGAACCCCACATGAAAAGGCTTGAAGCTGATCAACTCTTCCTTGAGTATGCTACTCCAACTCCTACTGCCCCGGCCACCCCATAA

Coding sequence (CDS)

ATGAAGCTTGAAATTGACATCATCTCAGAGGATTTAATCAAACCTGCTACTCCCACGCCTCCCCACCTCCGCCATTACCCCCTTTCCTTCATCGACCAAATCACCGTTCACATTTATGCTCCCGCCCTCTACTTCTACCGACCCACCCACGCCGGAGATCCCGATTTCGCCATCCACTTCCGGAACCGCCTCCGGAGCACTCTCTCCCTCGTCCTCTCCCACTACCCTCCACTCGCCGGCCGCCCCAACTACGACTCCGCCTTTGTGGACTGCAATGACACGGGGGCCTTATTTCGGGAAGCGCGAGTGAATACAAAACTCGCTGACGTGGTGGAATATGCCCAACCGGACGATCTGAACCGCCTGTTCCCGGTCGAGTTGGACCAGTTCAACGAAGAGCTGATGGCGGTTCAGTTCACGGAGTTCGCGTGCGGTGGGGTGGCGGTGGCGTCGTGCATCTCGCATAAGATTGCGGATGCGATGACGCTGTTTTCGCTGAATAATAACTGGGCGGCGATGGCGCGTGGAGTGAAGGGGGTTTTCAAACCGCACATGGAAGGGGCTAAGTTGTTCCCGGCGAAAACGACGAGGTATGATACGGCGAAGACGATTCTGAGAAACAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAGGCGATTCGCGAGAAATACGGAGAGAATGGCGCAGCGCACCGACCTTCACGGGTCGAGTCATTGACGGCTTTTCTGTACGGACGGTTTCTTGCGGCTCTTAAAGAGGGCTCTTCTTCTGAAGTTCCGAGCGACAGGGCATGTCTAGTGAACTACACAGTGAATCTGCGGCCAAAAATGGAGCCGCCACTTCCGGGCGACGCCTTTGGGAACTACTATTTCAACGCCATGATCTTCCCGTCCCCGGACATTCTGAACGACCCGTACTGCCACGGTCTAGTGAACCGGCTGAGAGAAGAAACCAACAAGCTGAACGCCCAGACAGTGAAGTACATTCTGAATGAAGACCAGAGGCTGATGGAGAGCGTGGAGGAGATTGCTTCTAAGTTCGTGAAGGGAGAGATGATTTCGTGTGCGTTCACTAGCATCTGCCGGTTTCCCCTTTATGACGTGGATTTCGGGTGGGGCCGGCCTGTGTGGGTGACTTTTCCGGCGCTGTGGTTCAAGAATCTGGTGACGTTGTTGGATTCCAAAGAGGCCGGCGGCATTGATGCGATTGTTCACCTTGAGGAACCCCACATGAAAAGGCTTGAAGCTGATCAACTCTTCCTTGAGTATGCTACTCCAACTCCTACTGCCCCGGCCACCCCATAA

Protein sequence

MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEADQLFLEYATPTPTAPATP

Homology

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-53
Identity = 140/433 (32.33%), Postives = 227/433 (52.42%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           M  ++ I+SE+LI+P++PTP  L+ + LS +DQ+ +  + P + FY      + D A   
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRS 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
            N L+ +LS VL+ + PLAGR N +S+ VDCND+G  F EARV+++L++ ++    D+LN
Sbjct: 61  EN-LKRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAIDELN 120

Query: 121 RLFPVELDQFNEEL-------MAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 180
           +  P +     EE        +AV+ + F CGG A+  CISHKIADA++L +  N+W A 
Sbjct: 121 QYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTAT 180

Query: 181 ARGVKGVFKPHME-GAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA 240
            +    + +P+ + G+  FP   +          N V +RFVF+  K+EA++ +   +  
Sbjct: 181 CQEETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSAT 240

Query: 241 -AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGN 300
                SRV+ + A ++ +F+    + + ++  +    +    VNLR +M PP P  A GN
Sbjct: 241 EVKNSSRVQIVIAVIWKQFI----DVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGN 300

Query: 301 YYFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFV 360
               A          D     LV  L+    K++ + VK        L + V  +  +  
Sbjct: 301 IATMAYAVAE----EDKDFSDLVGPLKTSLAKIDDEHVK-------ELQKGVTYLDYEAE 360

Query: 361 KGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEE 420
             E+ S  F+S CR   YD+DFGWG+PV V    +  KNLV L+D++   G++A + + E
Sbjct: 361 PQELFS--FSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAE 414

Query: 421 PHMKRLEADQLFL 425
             M  L +D L L
Sbjct: 421 DEMSMLSSDFLSL 414

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 5.5e-48
Identity = 136/435 (31.26%), Postives = 218/435 (50.11%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           M  +++ +SE+LI P++PTP  L+ Y +S +DQ+ +  + P + FY      + D A   
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPA-QT 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLA----DVVEYAQP 120
              L+ +LS VL+H+ PLAGR N +S+ VDCND+G  F EARV  +L+    +VVE  + 
Sbjct: 61  SQHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVELEKL 120

Query: 121 DDL--NRLFPVELDQFNEEL-MAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 180
           D    +  +P    + NE++ +AV+ + F CGG A+   +SHKIAD ++L +  N W A 
Sbjct: 121 DQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTAT 180

Query: 181 ARGVKGVFKPHME-GAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA 240
            RG   +  P+ +  A+ FP              N V +RFVF+  K+ A+R +      
Sbjct: 181 CRGETEIVLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSASE 240

Query: 241 AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNY 300
               SRV+ + A+++   +    + + ++  +    +V   VNLR +M PPLP  A GN 
Sbjct: 241 EKNFSRVQLVVAYIWKHVI----DVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGN- 300

Query: 301 YFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVK 360
               ++F + D   D     L+  LR    K           ED    E ++ +   +  
Sbjct: 301 -IATLLFAAVDAEWDKDFPDLIGPLRTSLEK----------TEDDHNHELLKGMTCLYEL 360

Query: 361 GEMISCAFTSICRFPLYDVDFGWGRPV---WVTFPALWFKNLVTLLDSKEAGGIDAIVHL 420
                 +FTS CR   YD+DFGWG+P+     TFP    +N   L+D++   G++A + +
Sbjct: 361 EPQELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPK---RNAALLMDTRSGDGVEAWLPM 414

Query: 421 EEPHMKRLEADQLFL 425
            E  M  L  + L L
Sbjct: 421 AEDEMAMLPVELLSL 414

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.0e-46
Identity = 132/443 (29.80%), Postives = 217/443 (48.98%), Query Frame = 0


Query: 5   IDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFY-----RPTHAGDPDFAIH 64
           + +  +  IKP++ TP  LR + LSFIDQ   ++Y P  +FY     R   + + D   H
Sbjct: 10  VSVCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 65  FRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDL 124
             + L+++LS  L  Y P AG+   D+A VDCND GA F   R+   +++++++      
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLK-DNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLA 129

Query: 125 NRL-FPVELDQFNE----ELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMA 184
             +  P +L   N      L+ VQ ++F CGG+A++ C SHKI D  +L +  N+W+++ 
Sbjct: 130 ESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVT 189

Query: 185 RG---VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNR---VARRFVFEGSKVEAIREKYG 244
           R       V  P   G  +F  KT    T   IL +    V +R +F   K++A+R K  
Sbjct: 190 RDHTTTTLVPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAKVA 249

Query: 245 ENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDA 304
           E      P+R E ++A L+        + SSS +PS    LV++ +N+R  ++P LP + 
Sbjct: 250 EESGVKNPTRAEVVSALLF----KCATKASSSMLPSK---LVHF-LNIRTMIKPRLPRNT 309

Query: 305 FGNYYFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIAS 364
            GN   +++       + D     LV  LR+E   +     K  + +++ ++E VE +  
Sbjct: 310 IGN--LSSIFSIEATNMQDMELPTLVRNLRKE---VEVAYKKDQVEQNELILEVVESMRE 369

Query: 365 KFVKGEMIS-----CAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGI 424
             +  E +         +++C++P Y VDFGWGRP  V       KN   L D K   G+
Sbjct: 370 GKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGV 429

Query: 425 DAIVHLEEPHMKRLEADQLFLEY 427
           +A V L +  M   E ++  LE+
Sbjct: 430 EARVMLHKQQMSEFERNEELLEF 438

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 8.9e-46
Identity = 135/448 (30.13%), Postives = 233/448 (52.01%), Query Frame = 0


Query: 5   IDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPAL---YFYRPTHAGDPDFAIHFR 64
           ++I+S+  IKP++PTP  LR Y LS IDQ T+     +L   Y Y P+H           
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQ-TIDSEVTSLAFFYTYNPSHESS-----KIG 65

Query: 65  NRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLNR 124
           + L+++LS  L  Y   AGR   ++ ++DCND G  F E R++ ++ D+++  +    + 
Sbjct: 66  DLLKNSLSKTLVSYYQFAGR-LIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDL 125

Query: 125 LFPVELDQFNEE-LMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 184
           + P  +   +E+ L+ VQ + F CGG+A+    SHK++D ++      +WA+ +  +   
Sbjct: 126 VLPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS-STSLSTF 185

Query: 185 FKPH--MEGAKLFPAKTTRYDTAKTIL--RNRVARRFVFEGSKVEAIREKYGENGAAHRP 244
            KP   +    +FP +  +  + K+I+  +  + +RFVF    +E ++ K  E G   +P
Sbjct: 186 HKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYG-IQKP 245

Query: 245 SRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNA 304
           SRVE +TAFL        +  ++ ++P  +   +   VNLRP +   LP ++ GN +  +
Sbjct: 246 SRVEVVTAFL-------CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIF--S 305

Query: 305 MIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMI 364
             F   D   D     L+++LR    KL    ++ I+++++   ES  +  +K +    I
Sbjct: 306 FYFCINDEGMDNQFSALISKLRNGKQKL----LENIISKEKLTYESQMQELTKCLDQLNI 365

Query: 365 SCAFT----SICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEP 424
           S   T    S CRFP+YD+DFGWG+P+ V+      K+L+ L+DS E  GI+A++ +EE 
Sbjct: 366 SSLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITMEEK 425

Query: 425 HMKRLEADQLFLEYA---TPTPTAPATP 438
            M   E ++    +A   +P P A   P
Sbjct: 426 KMAAFEKNEELRSFAYLDSPEPEALIIP 429

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 143/463 (30.89%), Postives = 219/463 (47.30%), Query Frame = 0


Query: 5   IDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF--RN 64
           +++IS++ IKP  PTP  LR+Y +S +DQ +  +Y P + FY      +   + H    +
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 65  RLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLNRL 124
            L+ +LS  L H+ P+AGR   D+  VDCND G  F E R+  ++ D +  +    L+ L
Sbjct: 69  LLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDA-HLSLL 128

Query: 125 FPVELDQFN---EELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAA---MARG 184
            P E+   N   E  + VQ   F CGG A+  CIS+KIADA T+ +   + A    +AR 
Sbjct: 129 LPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIARR 188

Query: 185 VKGV--------FKPHMEGAKLFP-----AKTTRYDTA-KTILRNRVARRFVFEGSKVEA 244
              +          P  +   LFP     A    Y T   T +   V++RFVF+ +K+ +
Sbjct: 189 GSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKITS 248

Query: 245 IREK----YGENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRP 304
            REK      +    HRP+RVE ++A ++   + +   GS S         V + +N R 
Sbjct: 249 AREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPGSIS--------TVTHAMNFRK 308

Query: 305 KMEPPLPGDAFGN--YYFNAMIFPSPDILNDPYCHGLVNRLREETNKLN--AQTVKYILN 364
           KM+PPL   +FGN      A++  +     +P    + +   EE   L+  +  V  +  
Sbjct: 309 KMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVALLRR 368

Query: 365 EDQRLMES---VEEIASKFVKGEMISCA-------------FTSICRFPLYDVDFGWGRP 419
           E  ++      +E+I  KF+ G   S A              TS C+F  Y+ DFGWG P
Sbjct: 369 EIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYEADFGWGTP 428

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.6e-258
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN
Sbjct: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFLEYATPTPTAPATP
Sbjct: 421 QLFLEYATPTPTAPATP 437

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 5.6e-245
Identity = 415/437 (94.97%), Postives = 425/437 (97.25%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLR YPLSFIDQITVHIYAPALYFY+P  AGDPDFAI F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLS+VLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYA+PDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKT  YDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAF+YGRFLAALK+GSSS+VPSDR CLVNYTVNLR KMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFMYGRFLAALKDGSSSQVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDILNDPYC+GLV+RLREETNKLNAQTVKYILNEDQ LMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILNDPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVT LDSKE GGIDAIVHLEEPHMK+LEAD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPHMKKLEAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFL+YATPTPTAP TP
Sbjct: 421 QLFLQYATPTPTAPPTP 437

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 9.4e-192
Identity = 331/441 (75.06%), Postives = 377/441 (85.49%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG----DPDF 60
           MKLE+DIISE+LIKP+TPTPPHLRHY LSFIDQITVHIYAPALYFY+ T  G    D DF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  AIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQP 120
           A+ F+NRLRSTL  VLSHYPPLAGRPNY S+F+DCNDTG  FREA VN++LADV+++AQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGEN----GA 240
           VKGVFKPHMEGAK+FP K   YD+A TI+RNRV+RRFVF+ SKVEAIR KY EN      
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNY 300
            H+PSR+ESLTAF+Y RFLAA K    SE+ +D + LVNYTVNLRPKM PPLP DAFGNY
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKH--DSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNY 300

Query: 301 YFNAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFV 360
           YFN MIFPSP+ LN D  C+GLV +LREETNK++ +  K  LNED+ LM++V+E+ASK V
Sbjct: 301 YFNVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVV 360

Query: 361 KGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEE 420
            GE++SCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+  GIDAIVHLEE
Sbjct: 361 SGEIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEE 420

Query: 421 PHMKRLEADQLFLEYATPTPT 433
            +M +LE D++F++YATP PT
Sbjct: 421 RYMNKLEGDEVFMKYATPIPT 439

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.8e-190
Identity = 331/445 (74.38%), Postives = 378/445 (84.94%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG----DPDF 60
           MKLE+DIISE+LIKP+TPTPPHLRHY LSFIDQITVHIYAPALYFY+ T  G    D DF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  AIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQP 120
           A+ F+NRLRSTL  VLSHYPPLAGRPNY S+F+DCNDTG  FREARVN++LADV+++AQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIADAM LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAA--- 240
           VKGVFKPHMEGAK+FP K   YDTA TI+RNRV+RRFVF+ SK+EAIR KY EN      
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 HRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYY 300
           +RPSR+ESLTAF+Y RFLAA K  S+  + +D + LVNYTVNLRPKM PPLP DAFGNYY
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSA--IRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYY 300

Query: 301 FNAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVK 360
           FN MIFPSP+ LN D  C+ LV +LREETNK++ +  K  LNED+ LME+V+E+ASK   
Sbjct: 301 FNVMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVAN 360

Query: 361 GEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEP 420
           GE+ISCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+  G+DAIVHLEE 
Sbjct: 361 GEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEER 420

Query: 421 HMKRLEADQLFLEYATPTPTAPATP 438
           +M +LE D++F+ YATP PT P++P
Sbjct: 421 YMNKLEGDEVFMNYATPLPT-PSSP 442

[Close]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.5e-184
Identity = 321/426 (75.35%), Postives = 363/426 (85.21%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG----DPDF 60
           MKLE+DIISE+LIKP+TPTPPHLRHY LSFIDQITVHIYAPALYFY+ T  G    D DF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  AIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQP 120
           A+ F+NRLRSTL  VLSHYPPLAGRPNY S+F+DCNDTG  FREARVN++LADV+++AQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIADAM LFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAA--- 240
           VKGVFKPHMEGAK+FP K   YDTA TI+RNRV+RRFVF+ SK+EAIR KY EN      
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 HRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYY 300
           +RPSR+ESLTAF+Y RFLAA K  S+  + +D + LVNYTVNLRPKM PPLP DAFGNYY
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSA--IRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYY 300

Query: 301 FNAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVK 360
           FN MIFPSP+ LN D  C+ LV +LREETNK++ +  K  LNED+ LME+V+E+ASK   
Sbjct: 301 FNVMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVAN 360

Query: 361 GEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEP 419
           GE+ISCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+  G+DAIVHLEE 
Sbjct: 361 GEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEER 420

[Close]

BLAST of CmaCh12G000480.1 vs. NCBI nr
Match: XP_023002585.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 437/437 (100.00%), Postives = 437/437 (100.00%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN
Sbjct: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFLEYATPTPTAPATP
Sbjct: 421 QLFLEYATPTPTAPATP 437

[Close]

BLAST of CmaCh12G000480.1 vs. NCBI nr
Match: XP_022951641.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 856.3 bits (2211), Expect = 1.2e-244
Identity = 415/437 (94.97%), Postives = 425/437 (97.25%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLR YPLSFIDQITVHIYAPALYFY+P  AGDPDFAI F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLS+VLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYA+PDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKT  YDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAF+YGRFLAALK+GSSS+VPSDR CLVNYTVNLR KMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFMYGRFLAALKDGSSSQVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDILNDPYC+GLV+RLREETNKLNAQTVKYILNEDQ LMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILNDPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVT LDSKE GGIDAIVHLEEPHMK+LEAD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPHMKKLEAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFL+YATPTPTAP TP
Sbjct: 421 QLFLQYATPTPTAPPTP 437

[Close]

BLAST of CmaCh12G000480.1 vs. NCBI nr
Match: KAG7020113.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854.4 bits (2206), Expect = 4.4e-244
Identity = 414/437 (94.74%), Postives = 424/437 (97.03%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLR YPLSFIDQITVHIYAPALYFY+P  AGDPDFAI F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLS+VLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYA+PDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKT  YDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAF+YGRFLAALK+GSSSEVPSDR CLVNYTVNLR KMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFMYGRFLAALKDGSSSEVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDILNDPYC+GLV+RLREETNKLNAQTVKYILNEDQ LMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILNDPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVT LDSKE GGIDAIVHLEEPHM +L+AD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPHMNKLQAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFL+YATPTPTAP TP
Sbjct: 421 QLFLQYATPTPTAPPTP 437

[Close]

BLAST of CmaCh12G000480.1 vs. NCBI nr
Match: KAG6585195.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 414/437 (94.74%), Postives = 423/437 (96.80%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           MKLEIDIISEDLIKPATPTPPHLR YPLSFIDQITVHIYAPALYFY+P  AGDPDFAI F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120
           RNRLRSTLS+VLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYA+PDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGV 180
           RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKG 
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVKGG 180

Query: 181 FKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240
           FKPHMEGAKLFPAKT  YDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE
Sbjct: 181 FKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGAAHRPSRVE 240

Query: 241 SLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYFNAMIFP 300
           SLTAF+YGRFLAALK+GSSSEVPSDR CLVNYTVNLR KMEPPLPGDAFGNYYFNAMIFP
Sbjct: 241 SLTAFMYGRFLAALKDGSSSEVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYFNAMIFP 300

Query: 301 SPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKGEMISCAF 360
           SPDIL DPYC+GLV+RLREETNKLNAQTVKYILNEDQ LMESVEEIASKFVKGEMISCAF
Sbjct: 301 SPDILKDPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKGEMISCAF 360

Query: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPHMKRLEAD 420
           TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVT LDSKE GGIDAIVHLEEPHMK+LEAD
Sbjct: 361 TSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPHMKKLEAD 420

Query: 421 QLFLEYATPTPTAPATP 438
           QLFL+YATPTPTAP TP
Sbjct: 421 QLFLQYATPTPTAPPTP 437

[Close]

BLAST of CmaCh12G000480.1 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 679.5 bits (1752), Expect = 1.9e-191
Identity = 331/441 (75.06%), Postives = 377/441 (85.49%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG----DPDF 60
           MKLE+DIISE+LIKP+TPTPPHLRHY LSFIDQITVHIYAPALYFY+ T  G    D DF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  AIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQP 120
           A+ F+NRLRSTL  VLSHYPPLAGRPNY S+F+DCNDTG  FREA VN++LADV+++AQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180
           DDLNRLFPVELD+FNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGEN----GA 240
           VKGVFKPHMEGAK+FP K   YD+A TI+RNRV+RRFVF+ SKVEAIR KY EN      
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNY 300
            H+PSR+ESLTAF+Y RFLAA K    SE+ +D + LVNYTVNLRPKM PPLP DAFGNY
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKH--DSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNY 300

Query: 301 YFNAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFV 360
           YFN MIFPSP+ LN D  C+GLV +LREETNK++ +  K  LNED+ LM++V+E+ASK V
Sbjct: 301 YFNVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVV 360

Query: 361 KGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEE 420
            GE++SCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+  GIDAIVHLEE
Sbjct: 361 SGEIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEE 420

Query: 421 PHMKRLEADQLFLEYATPTPT 433
            +M +LE D++F++YATP PT
Sbjct: 421 RYMNKLEGDEVFMKYATPIPT 439

[Close]

BLAST of CmaCh12G000480.1 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.9e-76
Identity = 163/441 (36.96%), Postives = 262/441 (59.41%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYP----LSFIDQITVHIYAPALYFY-RPTHAGDPD 60
           M LEI + S++L+K   P+P +L H P    LSF+DQ+   I+ P L+FY   T+  D +
Sbjct: 1   MGLEITVTSQELVK---PSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKE 60

Query: 61  FAIHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQ 120
            + H    ++S+LS +L+ Y PLAGR       V CND G  F EA+ +  ++ ++E   
Sbjct: 61  RSDH----IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPN 120

Query: 121 PDDLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMAR 180
           P++LN+L P E  + ++  + VQ T F CGG+A+   +SHK+ DA++     N+WAA AR
Sbjct: 121 PNELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFAR 180

Query: 181 G-VKGVFKPHMEGAKLF-PAKTTRYDTAKTILR-NRVARRFVFEGSKVEAIREKYGENGA 240
           G    +  P  + AK+F P      + A  I + N V RRFVF  S VE++RE++  N  
Sbjct: 181 GQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKK 240

Query: 241 AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNY 300
             R +RVE L+ F++ RF+A+    ++ +  + +   + + VNLR + +P +P + FGN 
Sbjct: 241 I-RATRVEVLSVFIWSRFMAS----TNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNI 300

Query: 301 YFNAMIFPSPDI-LNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFV 360
              ++  P   I  ND     LV+++REE  K++A  VK +  +++  +E + + AS FV
Sbjct: 301 MRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFV 360

Query: 361 KGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEE 420
            GE++S +FTS+C+FP+Y+ DFGWG+P+WV    + +KNLV  +D+KE  GI+A ++L++
Sbjct: 361 NGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQ 420

Query: 421 PHMKRLEADQLFLEYATPTPT 433
             M R EAD+  L Y +  P+
Sbjct: 421 NDMSRFEADEELLRYVSSNPS 429

[Close]

BLAST of CmaCh12G000480.1 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.5e-66
Identity = 144/445 (32.36%), Postives = 256/445 (57.53%), Query Frame = 0


Query: 3   LEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHFRN 62
           + +D++S D+IKP++PTP HL+ + LS ++Q+   I+ P ++FY   ++  P   +    
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQM-- 60

Query: 63  RLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLNRL 122
            L+ +LS  L+H+ PLAGR    +  +DCND+GA F EARVN+ L++++     D L +L
Sbjct: 61  -LKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQL 120

Query: 123 FPVELD--QFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM-ARG-VK 182
            P  +D  +    L+  Q + F CG +++  CISHK+ADA ++     +WAA+ +RG +K
Sbjct: 121 IPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIK 180

Query: 183 GVFKPHMEGAKLFP----AKTTRYDTA--KTILRNRVARRFVFEGSKVEAIREKYGENGA 242
            +  P  +  K+FP    ++T+       + ++   +++RF+F+ S ++A++ K   +  
Sbjct: 181 TIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAK-ASSFE 240

Query: 243 AHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNY 302
            ++P+RVE+++A ++   + A +  S +  PS    ++  +V+LR ++ PP   ++ GN 
Sbjct: 241 VNQPTRVEAVSALIWKSAMKATRTVSGTSKPS----ILANSVSLRSRVSPPFTKNSIGNL 300

Query: 303 --YFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYIL---NEDQRLMESVEEIA 362
             YF A    + + +N      LV+++R+   +     +  ++   N  + +    +E  
Sbjct: 301 VSYFAA---KAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAG 360

Query: 363 SKFVKGEMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIV 422
                G+     F+S CRF LY+ DFGWG+PVWV FP++  KN+VTLLD+KEAGGI+A V
Sbjct: 361 DMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWV 420

Query: 423 HLEEPHMKRLEADQLFLEYATPTPT 433
           +L E  M   E D+  L++A+  P+
Sbjct: 421 NLNEQEMNLFEQDRELLQFASLNPS 433

[Close]

BLAST of CmaCh12G000480.1 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-54
Identity = 146/447 (32.66%), Postives = 230/447 (51.45%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           M+  ++I+S +++KP++PTP   R   LS +D ++  +Y  AL FY    A DP   + F
Sbjct: 1   MEKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFY----AADPQNLLGF 60

Query: 61  RN-----RLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQ 120
                  +L+ +LS  L  + PLAGR     +FV+CND GA+F EARV+  L++ ++   
Sbjct: 61  STEETSLKLKKSLSKTLPIFYPLAGR--IIGSFVECNDEGAVFIEARVDHLLSEFLKCPV 120

Query: 121 PDDLNRLFPVEL---DQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAA 180
           P+ L  L PVE    +     ++ +Q   F+CGG+ +  C+SHKI DA +L      WA 
Sbjct: 121 PESLELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAE 180

Query: 181 MARGVKGVFKPHMEGAKLFPAKTTRYDTAKTILRNR------VARRFVFEGSKVEAIREK 240
            +RG+     P    +++FP       +     +        V +RFVF+ SK++ +R K
Sbjct: 181 SSRGLGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 240

Query: 241 YGENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPG 300
              N     P+RVE++TA L+ R +  +   SS    + R  ++   VNLR K++  L  
Sbjct: 241 ASRN-LVKNPTRVEAVTA-LFWRCVTKVSRLSSL---TPRTSVLQILVNLRGKVD-SLCE 300

Query: 301 DAFGNYYFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEI 360
           +  GN   + MI  + +   +     +V+ +R    ++ +   K +     R+ E +EEI
Sbjct: 301 NTIGN-MLSLMILKNEEAAIE-RIQDVVDEIR-RAKEIFSLNCKEMSKSSSRIFELLEEI 360

Query: 361 ASKFVKG-EMISCAFTSICRFPLYDVDFGWGRPVWVTFPAL-WFKNLVTLLDSKEAGGID 420
              + +G EM      S C+  LYD DFGWG+PVWVT      FKNL+ L+D+K+  GI+
Sbjct: 361 GKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIE 420

Query: 421 AIVHLEEPHMKRLEADQLFLEYATPTP 432
           A + L E  M   E DQ  LE A+  P
Sbjct: 421 AWITLTEEQMSLFECDQELLESASLNP 432

[Close]

BLAST of CmaCh12G000480.1 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-52
Identity = 145/455 (31.87%), Postives = 232/455 (50.99%), Query Frame = 0


Query: 3   LEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHFRN 62
           ++++ I++++IKP++ TP  L+   LS  D I   +Y  A  FY       P+ + H   
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSH--- 63

Query: 63  RLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNT-KLADVVEYAQPDDLNR 122
           +L+++L+  L+ + PLAGR       +DCND GA+F +ARVN   L+D +       L +
Sbjct: 64  KLKTSLAETLTKFYPLAGR--IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQ 123

Query: 123 LFPVEL--DQFNE----ELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMAR 182
           L P+++  D +       L+ V+ T F CGG+A+  CI+HKIADA ++ +   +WAAMAR
Sbjct: 124 LLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 183 GVKG--VFKPHMEGAKLFPAKTTRY-----DTAKTILRNRVARRFVFEGSKVEAIREKYG 242
           G  G  V  P    A  +P     +     + A  I  +++ +RFVF  SK+E +R K  
Sbjct: 184 GEAGDIVADPVFAAANFYPPANELFKFPVDENANKI--SQITKRFVFSASKLEELRTKAA 243

Query: 243 ENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDA 302
                 RP RVES+TA L+  F+AA    SS+    D   L+  + NLRPK+   L    
Sbjct: 244 SEDFVGRPKRVESVTALLWKAFVAA---ASSNTNACDMKVLIQPS-NLRPKIPSLLAESL 303

Query: 303 FGNYYFNAMI--------FPSPDILNDPYCHG--LVNRLREETNKLNAQTVKYILNEDQR 362
            GN  F++++              + D    G  L N ++EE    +A     + N    
Sbjct: 304 IGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLAN---L 363

Query: 363 LMESVEEIASKFVKGEMISCAFTSICRFPLYDVDFGWGRPVWVT-FPALWFKNLVTLLDS 422
           ++ +  +++ +  +   +S    S C+ PLY+  FGWG PVW+    A   +N+  L+DS
Sbjct: 364 MLTNYSKLSYETHEPYTVS----SWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDS 423

Query: 423 KEAGGIDAIVHLEEPHMKRLEADQLFLEYATPTPT 433
           K+  GI+A V L E +M  LE +   L +A+  P+
Sbjct: 424 KDGQGIEAFVTLPEENMLSLEQNTELLAFASVNPS 440

[Close]

BLAST of CmaCh12G000480.1 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-51
Identity = 145/459 (31.59%), Postives = 225/459 (49.02%), Query Frame = 0


Query: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60
           M ++++ IS+++IKP++PTP +L+   LS  D I   +Y  A  FY        +   H 
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQE---HT 63

Query: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNT-KLADVVEYAQPDDL 120
            ++L+++LS  L+ + PLAGR       VDC D GA+F +ARVN   L + ++    D L
Sbjct: 64  SHKLKTSLSETLTKFYPLAGR--ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDAL 123

Query: 121 NRLFPVE------LDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 180
            +L P++      +      L+ V+ T F CGG+A+  CI+HKIADA ++ +   +WAA 
Sbjct: 124 QQLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAAT 183

Query: 181 ARG---VKGVFKPHMEGAKLFPAKTTRYD---TAKTILRNRVARRFVFEGSKVEAIREKY 240
           ARG      +  P   GA  +P     +      +   R+ + +RFVFE SKVE +R K 
Sbjct: 184 ARGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKA 243

Query: 241 GENGAAHRPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGD 300
                  +P+RVES+TA ++  F+A     SS     D   LV    NLR K+   L   
Sbjct: 244 ASEETVDQPTRVESVTALIWKCFVA-----SSKTTTCDHKVLVQ-LANLRSKIPSLLQES 303

Query: 301 AFGNYYFNAMIFPSPDILNDPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIA 360
           + GN  F++++  S     +      V  LR++  +L       ++ ++    +S   I 
Sbjct: 304 SIGNLMFSSVVL-SIGRGGEVKIEEAVRDLRKKKEELGT-----VILDEGGSSDSSSMIG 363

Query: 361 SKFVKGEMISCA-----------FTSICRFPLYDVDFGWGRPVWV---TFPALWFKNLVT 420
           SK     + + +            +S C+ PLY+  FGW  PVWV     P L   NL  
Sbjct: 364 SKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVL--GNLAM 423

Query: 421 LLDSKEAGGIDAIVHLEEPHMKRLEADQLFLEYATPTPT 433
           L+DSK+  GI+A V L E +M   E +   L +AT  P+
Sbjct: 424 LIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPS 443

The following BLAST results are available for this feature:

BLAST of CmaCh12G000480.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2P1GIW7	1.5e-53	32.33	Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1	[more]
Q70PR7	5.5e-48	31.26	Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2	[more]
A0A2G3D6U1	4.0e-46	29.80	Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1	[more]
A0A2K8FQU5	8.9e-46	30.13	Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT...	[more]
I3PLR4	1.2e-45	30.89	(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ...	[more]

BLAST of CmaCh12G000480.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KPC8	7.6e-258	100.00	vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1	[more]
A0A6J1GI32	5.6e-245	94.97	vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1	[more]
A0A0A0LQS8	9.4e-192	75.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1	[more]
A0A5A7UYQ7	1.8e-190	74.38	Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5...	[more]
A0A1S3BD01	1.5e-184	75.35	vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1	[more]

BLAST of CmaCh12G000480.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002585.1	1.6e-257	100.00	vinorine synthase-like [Cucurbita maxima]	[more]
XP_022951641.1	1.2e-244	94.97	vinorine synthase-like [Cucurbita moschata]	[more]
KAG7020113.1	4.4e-244	94.74	Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585195.1	1.7e-243	94.74	Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_004143048.1	1.9e-191	75.06	stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical prot...	[more]

BLAST of CmaCh12G000480.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24430.1	4.9e-76	36.96	HXXXD-type acyl-transferase family protein	[more]
AT3G26040.1	3.5e-66	32.36	HXXXD-type acyl-transferase family protein	[more]
AT1G24420.1	2.4e-54	32.66	HXXXD-type acyl-transferase family protein	[more]
AT3G30280.1	6.5e-52	31.87	HXXXD-type acyl-transferase family protein	[more]
AT4G15390.1	1.4e-51	31.59	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 1..201 e-value: 2.2E-53 score: 182.7
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 206..424 e-value: 2.3E-49 score: 169.4
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 4..421 e-value: 9.0E-71 score: 238.8
None	No IPR available	PANTHER	PTHR31623	F21J9.9	coord: 2..429
None	No IPR available	PANTHER	PTHR31623:SF46	ANTHRANILATE N-BENZOYLTRANSFERASE	coord: 2..429

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh12G000480	CmaCh12G000480	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G000480.1:exon:697	CmaCh12G000480.1:exon:697	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G000480.1:cds	CmaCh12G000480.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh12G000480.1	CmaCh12G000480.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups